AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69608

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69608.t1 MTR_7g446520 56.040 298 106 3 58 350 30 307 2.77e-104 327
MS.gene69608.t1 MTR_7g446480 75.000 176 44 0 190 365 140 315 3.38e-87 281
MS.gene69608.t1 MTR_7g446510 74.432 176 45 0 190 365 155 330 2.87e-85 276
MS.gene69608.t1 MTR_7g446510 28.395 243 86 6 451 693 1 155 3.44e-17 84.3
MS.gene69608.t1 MTR_1g050570 76.744 172 40 0 717 888 1 172 5.25e-72 235
MS.gene69608.t1 MTR_7g028855 54.460 213 84 2 57 256 51 263 1.93e-69 238
MS.gene69608.t1 MTR_8g467420 78.462 130 28 0 37 166 2 131 7.72e-51 176
MS.gene69608.t1 MTR_1g070355 37.247 247 153 1 104 350 65 309 1.29e-48 182
MS.gene69608.t1 MTR_6g051425 33.462 260 169 3 123 379 27 285 1.47e-45 167
MS.gene69608.t1 MTR_5g076070 33.193 238 157 1 105 342 45 280 7.28e-41 152
MS.gene69608.t1 MTR_6g022070 36.207 174 111 0 410 583 1 174 8.17e-29 119
MS.gene69608.t1 MTR_0645s0010 39.370 127 74 1 488 614 1 124 3.83e-27 107
MS.gene69608.t1 MTR_4g035820 30.800 250 159 7 119 360 81 324 2.89e-24 109
MS.gene69608.t1 MTR_0402s0040 33.333 213 121 7 80 289 6 200 1.34e-22 102
MS.gene69608.t1 MTR_6g022080 28.488 172 123 0 104 275 76 247 3.99e-21 94.4
MS.gene69608.t1 MTR_8g468460 45.918 98 52 1 532 629 9 105 3.58e-20 87.0
MS.gene69608.t1 MTR_0558s0020 26.437 261 165 7 117 355 40 295 4.18e-20 92.4
MS.gene69608.t1 MTR_3g450850 27.234 235 157 6 119 342 53 284 1.86e-15 79.3
MS.gene69608.t1 MTR_1g008290 30.667 150 97 4 166 314 4 147 2.30e-14 71.6
MS.gene69608.t1 MTR_5g015480 31.694 183 116 5 53 234 2 176 1.33e-12 69.3
MS.gene69608.t1 MTR_8g446020 26.437 174 122 4 185 355 85 255 5.87e-12 67.4
MS.gene69608.t1 MTR_6g090505 24.373 279 162 9 158 418 21 268 6.24e-12 68.6
MS.gene69608.t1 MTR_6g053140 25.668 187 122 3 38 224 35 204 6.60e-12 66.2
MS.gene69608.t1 MTR_0060s0090 37.975 79 49 0 116 194 59 137 1.13e-11 64.3
MS.gene69608.t1 MTR_7g113010 27.411 197 111 6 64 260 422 586 2.46e-11 67.8
MS.gene69608.t1 MTR_7g074790 30.723 166 94 3 115 280 12 156 3.74e-11 64.3
MS.gene69608.t1 MTR_2g098880 34.579 107 68 2 114 219 62 167 4.03e-11 64.7
MS.gene69608.t1 MTR_2g098880 34.579 107 68 2 114 219 74 179 4.54e-11 64.7
MS.gene69608.t1 MTR_2g101460 30.400 125 87 0 104 228 46 170 7.94e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69608.t1 AT4G38180 31.194 670 428 11 56 708 21 674 1.62e-103 340
MS.gene69608.t1 AT4G15090 27.258 598 417 10 117 706 55 642 1.51e-80 278
MS.gene69608.t1 AT4G15090 27.258 598 417 10 117 706 55 642 1.51e-80 278
MS.gene69608.t1 AT4G15090 27.258 598 417 10 117 706 55 642 1.51e-80 278
MS.gene69608.t1 AT4G15090 27.258 598 417 10 117 706 55 642 1.51e-80 278
MS.gene69608.t1 AT4G15090 27.258 598 417 10 117 706 55 642 1.51e-80 278
MS.gene69608.t1 AT3G06250 28.317 618 386 14 110 719 187 755 9.36e-77 266
MS.gene69608.t1 AT3G06250 28.317 618 386 14 110 719 187 755 9.36e-77 266
MS.gene69608.t1 AT3G06250 28.317 618 386 14 110 719 187 755 9.36e-77 266
MS.gene69608.t1 AT5G18960 29.402 619 367 20 117 719 215 779 4.81e-72 254
MS.gene69608.t1 AT5G18960 29.402 619 367 20 117 719 215 779 4.81e-72 254
MS.gene69608.t1 AT3G22170 27.287 656 424 14 72 707 29 651 1.30e-71 253
MS.gene69608.t1 AT3G22170 27.287 656 424 14 72 707 29 651 1.30e-71 253
MS.gene69608.t1 AT1G76320 26.643 563 387 10 117 671 1 545 3.24e-65 233
MS.gene69608.t1 AT1G76320 26.643 563 387 10 117 671 1 545 3.24e-65 233
MS.gene69608.t1 AT1G76320 26.643 563 387 10 117 671 1 545 3.44e-65 232
MS.gene69608.t1 AT1G76320 26.643 563 387 10 117 671 1 545 4.37e-65 233
MS.gene69608.t1 AT1G80010 28.019 621 400 16 89 683 75 674 4.36e-64 230
MS.gene69608.t1 AT1G10240 26.875 640 406 16 85 689 14 626 3.53e-62 224
MS.gene69608.t1 AT1G10240 26.875 640 406 16 85 689 14 626 3.53e-62 224
MS.gene69608.t1 AT2G27110 26.290 620 408 14 58 670 2 579 1.86e-61 224
MS.gene69608.t1 AT2G27110 26.290 620 408 14 58 670 2 579 1.86e-61 224
MS.gene69608.t1 AT1G52520 27.711 581 389 10 117 683 89 652 4.89e-59 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 1 550 1.19e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 1 550 1.31e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 1.34e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 1.69e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 1.69e-58 215
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 26 571 1.82e-58 214
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 26 571 1.82e-58 214
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 26 571 1.90e-58 214
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 26 571 2.02e-58 214
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 26 571 2.02e-58 214
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 2.15e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 2.15e-58 215
MS.gene69608.t1 AT2G32250 24.795 609 393 11 117 719 42 591 2.15e-58 215
MS.gene69608.t1 AT4G19990 26.678 596 363 13 118 689 40 585 2.16e-58 214
MS.gene69608.t1 AT2G27110 29.716 387 264 5 289 670 51 434 1.82e-55 205
MS.gene69608.t1 AT4G19990 26.236 526 326 10 176 689 39 514 7.47e-53 197
MS.gene69608.t1 AT4G38170 27.387 398 276 6 280 670 8 399 8.67e-53 194
MS.gene69608.t1 AT5G28530 25.297 589 404 13 119 685 61 635 2.38e-52 195
MS.gene69608.t1 AT5G28530 24.958 589 402 13 119 685 61 631 2.48e-51 192
MS.gene69608.t1 AT5G28530 24.958 589 402 13 119 685 61 631 2.48e-51 192
MS.gene69608.t1 AT5G28530 22.396 576 374 12 119 685 61 572 6.75e-35 142
MS.gene69608.t1 AT5G28530 22.049 576 372 12 119 685 61 568 7.85e-34 139
MS.gene69608.t1 AT3G07500 33.333 126 80 3 94 218 14 136 5.03e-11 63.9

Find 153 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCATTCTTCTTCTTCTT+TGG 0.173135 3.4:+53128871 None:intergenic
GAAATTCTTAAACTTGAATT+TGG 0.176852 3.4:-53130800 MS.gene69608:CDS
TGTAAAGAAAACACCATTTC+TGG 0.203603 3.4:-53129826 MS.gene69608:CDS
ACCGCCATGAAGTCCATAAT+TGG 0.212290 3.4:+53128322 None:intergenic
TTGGCACAAGTCATGAATTT+TGG 0.213622 3.4:-53128272 MS.gene69608:CDS
CCATCTGTTACAACTGCTTT+TGG 0.217535 3.4:+53129942 None:intergenic
CATTCACAGACCATTATATT+TGG 0.220424 3.4:-53130047 MS.gene69608:CDS
TATTTACGTGATAAGTTCTT+TGG 0.228072 3.4:-53129660 MS.gene69608:CDS
GAATCATCAACTTCCTGTTC+TGG 0.232408 3.4:-53128299 MS.gene69608:CDS
TATATTTGGCGTTGCGTTGC+TGG 0.233448 3.4:-53130033 MS.gene69608:CDS
GAAGAATGAAAAGTCTGTTT+TGG 0.246187 3.4:-53130187 MS.gene69608:CDS
CAGCCAATTTACCCCAATTA+TGG 0.247084 3.4:-53128335 MS.gene69608:CDS
CGGAGCAATTTGAGGAGTTT+TGG 0.252202 3.4:-53129767 MS.gene69608:CDS
ACACCATTTCTGGAGGGTTT+TGG 0.254714 3.4:-53129816 MS.gene69608:CDS
TGTACGTTACAAAGCCAAAA+AGG 0.274297 3.4:-53130558 MS.gene69608:CDS
AGTCCATAATTGGGGTAAAT+TGG 0.292438 3.4:+53128332 None:intergenic
AATCAGAACCTGTCATATAA+TGG 0.299188 3.4:-53130399 MS.gene69608:CDS
CTGTCATATAATGGGATATA+TGG 0.309391 3.4:-53130390 MS.gene69608:CDS
TTCTGAACAACGATTCTAAT+GGG 0.314235 3.4:-53129119 MS.gene69608:CDS
AGCACAATTTACAGAGATTT+CGG 0.314466 3.4:-53128438 MS.gene69608:CDS
GCCTTGCTTCACAATTTGTT+CGG 0.318117 3.4:+53130585 None:intergenic
AACTTCCTGTTCTGGGCTCT+TGG 0.321078 3.4:-53128291 MS.gene69608:CDS
TACCCCAATTATGGACTTCA+TGG 0.333754 3.4:-53128326 MS.gene69608:CDS
CGGAGTGAAGATGGCGCTGC+TGG 0.341163 3.4:+53131903 None:intergenic
CCATTAGGAAAGGTGATGTT+AGG 0.342221 3.4:-53130721 MS.gene69608:CDS
CCTTGCTTCACAATTTGTTC+GGG 0.360091 3.4:+53130586 None:intergenic
TTTCTGAACAACGATTCTAA+TGG 0.360926 3.4:-53129120 MS.gene69608:CDS
TGATCACATACCAAAATGTT+TGG 0.362382 3.4:-53129178 MS.gene69608:CDS
ATGATTGAAGTTGCTAGATT+TGG 0.363762 3.4:-53129081 MS.gene69608:CDS
TTCGGAGACAGCTGATTTGC+CGG 0.364644 3.4:-53128420 MS.gene69608:CDS
GGAACACCACAAAGATTGTT+TGG 0.365076 3.4:-53131020 MS.gene69608:CDS
ATGAACTTGAAGAAAATGAT+TGG 0.365813 3.4:-53129725 MS.gene69608:CDS
ATTTAGGTGGGGTTAATTCA+TGG 0.368536 3.4:+53130498 None:intergenic
CTTGTGCTAACATTACCATT+AGG 0.373645 3.4:+53128485 None:intergenic
CGGGTCATAACCAAACATTT+TGG 0.385088 3.4:+53129168 None:intergenic
CAACGATTCTAATGGGGAAA+TGG 0.387707 3.4:-53129112 MS.gene69608:CDS
CCGCCATGAAGTCCATAATT+GGG 0.393287 3.4:+53128323 None:intergenic
CCTTTACTCTTCACAGGGAT+TGG 0.395830 3.4:+53128898 None:intergenic
ATCAGAACCTGTCATATAAT+GGG 0.403538 3.4:-53130398 MS.gene69608:CDS
GGTTGAACTGCAGTTGTTAC+CGG 0.408018 3.4:+53128401 None:intergenic
GAGACATGAAGCACTTATCT+AGG 0.412910 3.4:-53130640 MS.gene69608:CDS
AATCATCAACTTCCTGTTCT+GGG 0.413052 3.4:-53128298 MS.gene69608:CDS
TTTCCAAAACCCTCCAGAAA+TGG 0.414871 3.4:+53129813 None:intergenic
TGGTGTATTTAGGGACATAA+TGG 0.425462 3.4:-53129007 MS.gene69608:CDS
CAACGCAACGCCAAATATAA+TGG 0.427960 3.4:+53130037 None:intergenic
GGGTGCAAGTGTGTAAATTT+AGG 0.429032 3.4:+53130482 None:intergenic
CATCTCATCGCTTATGTGCC+TGG 0.431687 3.4:-53129875 MS.gene69608:CDS
TGAGAAATTAACAGATGATC+AGG 0.435117 3.4:-53128929 MS.gene69608:CDS
CGCATTCTTATGCAGATGCC+AGG 0.435360 3.4:+53129857 None:intergenic
AGAATTGGTATCTAACTCTA+TGG 0.436678 3.4:-53128628 MS.gene69608:CDS
GATCTCTGCTGTGTAAATCT+TGG 0.442124 3.4:+53129431 None:intergenic
CCTAACATCACCTTTCCTAA+TGG 0.442699 3.4:+53130721 None:intergenic
TTTGGGCTGATGGGAGTAGT+AGG 0.444063 3.4:-53130169 MS.gene69608:CDS
GAATACGATGTCAACATTGA+TGG 0.449029 3.4:-53130209 MS.gene69608:CDS
CATAAGCGATGAGATGCATC+AGG 0.456050 3.4:+53129882 None:intergenic
ACATGAACATGATGCAGAAT+TGG 0.463393 3.4:-53128643 MS.gene69608:CDS
TGAACTTGAAGAAAATGATT+GGG 0.463828 3.4:-53129724 MS.gene69608:CDS
AATGAGGGAAGCTATTAAAC+AGG 0.464184 3.4:-53129910 MS.gene69608:CDS
AGGCTTGGAGAGCGAGGTGG+TGG 0.470187 3.4:+53131879 None:intergenic
AAAGATGAAATTATTGAGGC+TGG 0.470276 3.4:-53129396 MS.gene69608:CDS
GGAGCACCTTTACTCTTCAC+AGG 0.470733 3.4:+53128892 None:intergenic
TAAATTGGCTGCATTTGCAT+TGG 0.473815 3.4:+53128347 None:intergenic
GTATTCTACTTCACTGGAAT+CGG 0.474179 3.4:+53130994 None:intergenic
AAGAATTTCATCAGAGGCAA+TGG 0.477228 3.4:+53130814 None:intergenic
ATCTGTGTATTCTACTTCAC+TGG 0.479751 3.4:+53130988 None:intergenic
GGGATATATGGTTGCTCAGA+AGG 0.480775 3.4:-53130378 MS.gene69608:CDS
TCAATCAACAATGCATTAGT+AGG 0.482447 3.4:-53130860 MS.gene69608:CDS
TTGTGCCAAGAGCCCAGAAC+AGG 0.484522 3.4:+53128286 None:intergenic
TGGCGCTGCTGGGTCGCACG+TGG 0.486275 3.4:+53131914 None:intergenic
GCAAGTGTGTAAATTTAGGT+GGG 0.497265 3.4:+53130486 None:intergenic
TCATACTGCAAGGCATTGTT+CGG 0.499503 3.4:-53128809 MS.gene69608:intron
GGTGGTGGACGGAGTGAAGA+TGG 0.501161 3.4:+53131894 None:intergenic
TTGCGAAAATATCGTGAGAA+AGG 0.510344 3.4:-53129321 MS.gene69608:CDS
CACCTCGCTCTCCAAGCCTC+AGG 0.510859 3.4:-53131875 MS.gene69608:intron
ATTTGCATTGGATTAATAGC+AGG 0.513071 3.4:+53128359 None:intergenic
CAAAACTCCTCAAATTGCTC+CGG 0.514523 3.4:+53129768 None:intergenic
GATGCTGAAGATGATGTTGA+TGG 0.520081 3.4:-53130929 MS.gene69608:CDS
GAAAGAAGAGGCAACGGAGT+GGG 0.522053 3.4:+53131954 None:intergenic
CGGCGTCCAAACAATCTTTG+TGG 0.524396 3.4:+53131014 None:intergenic
CATATATCCCATTATATGAC+AGG 0.525157 3.4:+53130391 None:intergenic
CAAGCAAAAGAAATATAAAG+AGG 0.527664 3.4:-53128462 MS.gene69608:CDS
CCAAAAGCAGTTGTAACAGA+TGG 0.529837 3.4:-53129942 MS.gene69608:CDS
AGTTAAAGATGAAATTATTG+AGG 0.530146 3.4:-53129400 MS.gene69608:CDS
CTTGATGCTCTTCAATCACA+TGG 0.535182 3.4:-53130422 MS.gene69608:CDS
TGCAAGTGTGTAAATTTAGG+TGG 0.535511 3.4:+53130485 None:intergenic
GTGATTGAAGAGCATCAAGT+TGG 0.536251 3.4:+53130426 None:intergenic
TGATTGAAGAGCATCAAGTT+GGG 0.536427 3.4:+53130427 None:intergenic
TATCGTTATGGAAGATGCAA+AGG 0.541216 3.4:-53130749 MS.gene69608:CDS
TGCATTAGTAGGTGAAAGGT+TGG 0.543116 3.4:-53130849 MS.gene69608:CDS
GAGAGAGAAAGAAGAGGCAA+CGG 0.543213 3.4:+53131948 None:intergenic
GCATTGGATTAATAGCAGGC+TGG 0.543520 3.4:+53128363 None:intergenic
GGAGTGAAGATGGCGCTGCT+GGG 0.543895 3.4:+53131904 None:intergenic
CCAATCCCTGTGAAGAGTAA+AGG 0.544969 3.4:-53128898 MS.gene69608:CDS
AGAAAGAAGAGGCAACGGAG+TGG 0.548447 3.4:+53131953 None:intergenic
AAGGGTGGATACGCTGATGT+AGG 0.558445 3.4:-53130359 MS.gene69608:CDS
TTGGAGAGCGAGGTGGTGGA+CGG 0.559403 3.4:+53131883 None:intergenic
TAGAAAACGAACCTGAGGCT+TGG 0.561009 3.4:+53131864 None:intergenic
AATGTTTGGTTATGACCCGT+TGG 0.566267 3.4:-53129164 MS.gene69608:CDS
AAACAATCGAGTCATATAAG+TGG 0.567873 3.4:-53130004 MS.gene69608:CDS
GGCGCTGCTGGGTCGCACGT+GGG 0.568721 3.4:+53131915 None:intergenic
AGATGGAGATGGATCAATGA+GGG 0.572748 3.4:-53129925 MS.gene69608:CDS
GAGTTTGTGTGCAACAAGCA+AGG 0.575972 3.4:-53130668 MS.gene69608:CDS
GCAGTTGTAACAGATGGAGA+TGG 0.579255 3.4:-53129936 MS.gene69608:CDS
CTCAAGAGCAAAGAATGTGT+GGG 0.580226 3.4:-53129363 MS.gene69608:CDS
GTAGTGTCTAAATTTGAAGA+GGG 0.581617 3.4:-53130527 MS.gene69608:CDS
AGCATATAACATTGGATCGG+TGG 0.585153 3.4:+53130232 None:intergenic
CAAATATAATGGTCTGTGAA+TGG 0.585642 3.4:+53130048 None:intergenic
CCCGAACAAATTGTGAAGCA+AGG 0.587968 3.4:-53130586 MS.gene69608:CDS
AAGACATATGTCAGAGCGAA+AGG 0.588375 3.4:-53129591 MS.gene69608:CDS
GGATATATGGTTGCTCAGAA+GGG 0.589834 3.4:-53130377 MS.gene69608:CDS
AAGAAAACACCATTTCTGGA+GGG 0.593121 3.4:-53129822 MS.gene69608:CDS
AACAATCGAGTCATATAAGT+GGG 0.594755 3.4:-53130003 MS.gene69608:CDS
GATAGCTTCACATTGAGATG+TGG 0.595390 3.4:+53129626 None:intergenic
TGTAGTGTCTAAATTTGAAG+AGG 0.597171 3.4:-53130528 MS.gene69608:CDS
TGAGAAAGGCATTGAAAGAG+AGG 0.597237 3.4:-53129307 MS.gene69608:CDS
ACAATGCATTAGTAGGTGAA+AGG 0.599354 3.4:-53130853 MS.gene69608:CDS
CGCCATGAAGTCCATAATTG+GGG 0.600485 3.4:+53128324 None:intergenic
ATACGCGAACAAGTCATTGT+GGG 0.602038 3.4:-53129691 MS.gene69608:CDS
TGATGAATCAGTTCAACAAG+AGG 0.603374 3.4:-53130885 MS.gene69608:CDS
AAAGAAAACACCATTTCTGG+AGG 0.604034 3.4:-53129823 MS.gene69608:CDS
GATGTTAGGAGCAATAGTAG+TGG 0.605946 3.4:-53130707 MS.gene69608:CDS
CAAGTGTGTAAATTTAGGTG+GGG 0.607267 3.4:+53130487 None:intergenic
TCTCAAGAGCAAAGAATGTG+TGG 0.610833 3.4:-53129364 MS.gene69608:CDS
TATATGGTTGCTCAGAAGGG+TGG 0.613989 3.4:-53130374 MS.gene69608:CDS
CGCAGGTGAGTCGTCAACAC+AGG 0.614146 3.4:+53129474 None:intergenic
TTCAGCATATAACATTGGAT+CGG 0.614583 3.4:+53130229 None:intergenic
GAGCACCTTTACTCTTCACA+GGG 0.616222 3.4:+53128893 None:intergenic
AACCTGAGGCTTGGAGAGCG+AGG 0.625854 3.4:+53131873 None:intergenic
AAGTTTAAGAATTTCATCAG+AGG 0.626746 3.4:+53130808 None:intergenic
GCACACGAGAGAGAAAGAAG+AGG 0.627682 3.4:+53131942 None:intergenic
AAATTAACAGATGATCAGGT+AGG 0.630827 3.4:-53128925 MS.gene69608:CDS
CATACGCGAACAAGTCATTG+TGG 0.631204 3.4:-53129692 MS.gene69608:CDS
TCGTATTCAGCATATAACAT+TGG 0.632685 3.4:+53130224 None:intergenic
TGTAGACTATTTGAGACACG+TGG 0.633840 3.4:-53129246 MS.gene69608:CDS
GGCTGGAACACATGAACAGT+AGG 0.634224 3.4:+53128380 None:intergenic
TCTGAACAACGATTCTAATG+GGG 0.634233 3.4:-53129118 MS.gene69608:CDS
TCAAGAGCAAAGAATGTGTG+GGG 0.636452 3.4:-53129362 MS.gene69608:CDS
ATGCGTGAAGTCATTAAAGA+CGG 0.636681 3.4:-53130296 MS.gene69608:CDS
GAAGTACAAGTCATACTGCA+AGG 0.642891 3.4:-53128819 MS.gene69608:CDS
ATTTGGCGTTGCGTTGCTGG+AGG 0.644650 3.4:-53130030 MS.gene69608:CDS
CCCAATTATGGACTTCATGG+CGG 0.651938 3.4:-53128323 MS.gene69608:CDS
GAACTTATCACGTAAATATG+CGG 0.655600 3.4:+53129665 None:intergenic
GATGAATCAGTTCAACAAGA+GGG 0.669122 3.4:-53130884 MS.gene69608:CDS
GTGATACTGAATACTCTACT+CGG 0.673362 3.4:-53128954 MS.gene69608:CDS
TAGTGTCTAAATTTGAAGAG+GGG 0.673727 3.4:-53130526 MS.gene69608:CDS
CATCACTCTCAATCGAACGC+AGG 0.676080 3.4:+53129457 None:intergenic
CTGAGGCTTGGAGAGCGAGG+TGG 0.685584 3.4:+53131876 None:intergenic
CACCCAGAAAATATACACAA+CGG 0.691486 3.4:+53130077 None:intergenic
ATATAATGGTCTGTGAATGG+TGG 0.703942 3.4:+53130051 None:intergenic
CAAGAGCAAAGAATGTGTGG+GGG 0.709093 3.4:-53129361 MS.gene69608:CDS
CAGATGGAGATGGATCAATG+AGG 0.711130 3.4:-53129926 MS.gene69608:CDS
ATAAGCGATGAGATGCATCA+GGG 0.712304 3.4:+53129883 None:intergenic
GTAGACTATTTGAGACACGT+GGG 0.732024 3.4:-53129245 MS.gene69608:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATTTTCGAAATTTTA+GGG - chr3.4:53129142-53129161 MS.gene69608:CDS 10.0%
!!! GTTTAAATTTTCGAAATTTT+AGG - chr3.4:53129141-53129160 MS.gene69608:CDS 15.0%
!!! TATGAATTTTATTATCGTTA+TGG - chr3.4:53129431-53129450 MS.gene69608:CDS 15.0%
!! AAAAAAGAATGGTGTATTTA+GGG - chr3.4:53131176-53131195 MS.gene69608:intron 20.0%
!! AAAAATAAAAGGAAACCTAA+TGG - chr3.4:53131692-53131711 MS.gene69608:intron 20.0%
!! AACCCTAATTAAAAAGAAAA+AGG + chr3.4:53128387-53128406 None:intergenic 20.0%
!! ACTAGGTTATAACAAAAAAA+AGG - chr3.4:53128776-53128795 MS.gene69608:intron 20.0%
!! AGTTAAAGATGAAATTATTG+AGG - chr3.4:53130792-53130811 MS.gene69608:CDS 20.0%
!! GAAATTCTTAAACTTGAATT+TGG - chr3.4:53129392-53129411 MS.gene69608:CDS 20.0%
!! GTTATATACTTGTTTAGTAT+TGG - chr3.4:53128810-53128829 MS.gene69608:CDS 20.0%
!!! AATTAGGGTTTTTTTGATTT+TGG - chr3.4:53128397-53128416 MS.gene69608:CDS 20.0%
!!! AATTGGAGATTATTTTTTTG+GGG - chr3.4:53128554-53128573 MS.gene69608:intron 20.0%
!!! ATTTATGCATAATTTTGAAC+AGG - chr3.4:53130636-53130655 MS.gene69608:CDS 20.0%
!!! CAATTGGAGATTATTTTTTT+GGG - chr3.4:53128553-53128572 MS.gene69608:intron 20.0%
!!! CTCCTTTTTCTTTTTAATTA+GGG - chr3.4:53128382-53128401 MS.gene69608:CDS 20.0%
!!! TCTCCTTTTTCTTTTTAATT+AGG - chr3.4:53128381-53128400 MS.gene69608:CDS 20.0%
!!! TTTCCATACATTCTAAAAAA+CGG + chr3.4:53130222-53130241 None:intergenic 20.0%
!!! TTTTGCTTGCAATAATATAT+TGG - chr3.4:53128988-53129007 MS.gene69608:CDS 20.0%
! AAGTTTAAGAATTTCATCAG+AGG + chr3.4:53129387-53129406 None:intergenic 25.0%
! ACAAAAAAAACATGACAACA+TGG + chr3.4:53130976-53130995 None:intergenic 25.0%
! ATGAACTTGAAGAAAATGAT+TGG - chr3.4:53130467-53130486 MS.gene69608:CDS 25.0%
! ATTATGAAATTGGCATTATG+AGG - chr3.4:53128461-53128480 MS.gene69608:CDS 25.0%
! CAAAAAAGAATGGTGTATTT+AGG - chr3.4:53131175-53131194 MS.gene69608:intron 25.0%
! CAAGCAAAAGAAATATAAAG+AGG - chr3.4:53131730-53131749 MS.gene69608:intron 25.0%
! GTCATTCACAGAAAAATAAA+AGG - chr3.4:53131681-53131700 MS.gene69608:intron 25.0%
! TATTTACGTGATAAGTTCTT+TGG - chr3.4:53130532-53130551 MS.gene69608:CDS 25.0%
! TGAACTTGAAGAAAATGATT+GGG - chr3.4:53130468-53130487 MS.gene69608:CDS 25.0%
! TGAATGTAACAGTGTTTAAT+TGG - chr3.4:53128431-53128450 MS.gene69608:CDS 25.0%
! TGGTTGATTGATTATGAAAT+TGG - chr3.4:53128451-53128470 MS.gene69608:CDS 25.0%
! TTCTTTAAGCCAAAAAGAAA+AGG - chr3.4:53131591-53131610 MS.gene69608:intron 25.0%
!! AAGAATGAAAAGTCTGTTTT+GGG - chr3.4:53130006-53130025 MS.gene69608:CDS 25.0%
!! AATCACAAAATCGCAAATTT+TGG + chr3.4:53128530-53128549 None:intergenic 25.0%
!! AATGTACTCAAATTTTACAC+CGG - chr3.4:53130405-53130424 MS.gene69608:CDS 25.0%
!! ACAAACATACCTTTTCTTTT+TGG + chr3.4:53131603-53131622 None:intergenic 25.0%
!! ATAAGCAAGTTAATTTTTGC+AGG - chr3.4:53131510-53131529 MS.gene69608:intron 25.0%
!! ATATACTTGTTTAGTATTGG+AGG - chr3.4:53128813-53128832 MS.gene69608:CDS 25.0%
!! TGCCATTTATATGTACATTT+GGG - chr3.4:53128893-53128912 MS.gene69608:CDS 25.0%
!!! AAATACACCATTCTTTTTTG+AGG + chr3.4:53131175-53131194 None:intergenic 25.0%
!!! AGGTCTGATTATTTTTGTTT+TGG - chr3.4:53130043-53130062 MS.gene69608:CDS 25.0%
!!! GCAATTGGAGATTATTTTTT+TGG - chr3.4:53128552-53128571 MS.gene69608:intron 25.0%
!!! TCATGTTTTTTTTGTGATGA+AGG - chr3.4:53130981-53131000 MS.gene69608:CDS 25.0%
AAACAATCGAGTCATATAAG+TGG - chr3.4:53130188-53130207 MS.gene69608:CDS 30.0%
AAAGAAGCCTCAAAAAAGAA+TGG - chr3.4:53131165-53131184 MS.gene69608:intron 30.0%
AAAGATGAAATTATTGAGGC+TGG - chr3.4:53130796-53130815 MS.gene69608:CDS 30.0%
AAATTAACAGATGATCAGGT+AGG - chr3.4:53131267-53131286 MS.gene69608:intron 30.0%
AACAATCGAGTCATATAAGT+GGG - chr3.4:53130189-53130208 MS.gene69608:CDS 30.0%
AAGACGTAGATTTCATAGTT+TGG + chr3.4:53129056-53129075 None:intergenic 30.0%
AATCAGAACCTGTCATATAA+TGG - chr3.4:53129793-53129812 MS.gene69608:CDS 30.0%
AGAATTGGTATCTAACTCTA+TGG - chr3.4:53131564-53131583 MS.gene69608:intron 30.0%
AGCACAATTTACAGAGATTT+CGG - chr3.4:53131754-53131773 MS.gene69608:intron 30.0%
ATCAGAACCTGTCATATAAT+GGG - chr3.4:53129794-53129813 MS.gene69608:CDS 30.0%
ATGATTGAAGTTGCTAGATT+TGG - chr3.4:53131111-53131130 MS.gene69608:intron 30.0%
ATTTGCATTGGATTAATAGC+AGG + chr3.4:53131836-53131855 None:intergenic 30.0%
CAAATATAATGGTCTGTGAA+TGG + chr3.4:53130147-53130166 None:intergenic 30.0%
CACCAAATTAGTACCAATTT+CGG + chr3.4:53128581-53128600 None:intergenic 30.0%
CACCCAAATGTACATATAAA+TGG + chr3.4:53128898-53128917 None:intergenic 30.0%
CATATATCCCATTATATGAC+AGG + chr3.4:53129804-53129823 None:intergenic 30.0%
CATTCACAGACCATTATATT+TGG - chr3.4:53130145-53130164 MS.gene69608:CDS 30.0%
CATTTATATGTACATTTGGG+TGG - chr3.4:53128896-53128915 MS.gene69608:CDS 30.0%
GAACTTATCACGTAAATATG+CGG + chr3.4:53130530-53130549 None:intergenic 30.0%
GTAGTGTCTAAATTTGAAGA+GGG - chr3.4:53129665-53129684 MS.gene69608:CDS 30.0%
TAGTGTCTAAATTTGAAGAG+GGG - chr3.4:53129666-53129685 MS.gene69608:CDS 30.0%
TAGTTATTAAAGCAGACACT+AGG - chr3.4:53128759-53128778 MS.gene69608:intron 30.0%
TCAATCAACAATGCATTAGT+AGG - chr3.4:53129332-53129351 MS.gene69608:CDS 30.0%
TCGTATTCAGCATATAACAT+TGG + chr3.4:53129971-53129990 None:intergenic 30.0%
TGAGAAATTAACAGATGATC+AGG - chr3.4:53131263-53131282 MS.gene69608:intron 30.0%
TGATCACATACCAAAATGTT+TGG - chr3.4:53131014-53131033 MS.gene69608:CDS 30.0%
TGTAAAGAAAACACCATTTC+TGG - chr3.4:53130366-53130385 MS.gene69608:CDS 30.0%
TGTAGTGTCTAAATTTGAAG+AGG - chr3.4:53129664-53129683 MS.gene69608:CDS 30.0%
TGTTGATATGAATCTGTTGA+GGG - chr3.4:53128608-53128627 MS.gene69608:CDS 30.0%
TTCAGCATATAACATTGGAT+CGG + chr3.4:53129966-53129985 None:intergenic 30.0%
TTCTGAACAACGATTCTAAT+GGG - chr3.4:53131073-53131092 MS.gene69608:intron 30.0%
TTGGTTCATTGTGTAATTCA+AGG - chr3.4:53128674-53128693 MS.gene69608:intron 30.0%
TTGTTGATATGAATCTGTTG+AGG - chr3.4:53128607-53128626 MS.gene69608:CDS 30.0%
TTTCTGAACAACGATTCTAA+TGG - chr3.4:53131072-53131091 MS.gene69608:intron 30.0%
! ATCCGTTGTGTATATTTTCT+GGG - chr3.4:53130113-53130132 MS.gene69608:CDS 30.0%
! CGATTTTGTGATTATGCAAT+TGG - chr3.4:53128537-53128556 MS.gene69608:intron 30.0%
! CTGCCATTTATATGTACATT+TGG - chr3.4:53128892-53128911 MS.gene69608:CDS 30.0%
! CTGTCATATAATGGGATATA+TGG - chr3.4:53129802-53129821 MS.gene69608:CDS 30.0%
! GAAGAATGAAAAGTCTGTTT+TGG - chr3.4:53130005-53130024 MS.gene69608:CDS 30.0%
! TATCCGTTGTGTATATTTTC+TGG - chr3.4:53130112-53130131 MS.gene69608:CDS 30.0%
!!! GAACCGTTTTTTAGAATGTA+TGG - chr3.4:53130216-53130235 MS.gene69608:CDS 30.0%
AACACAATTGAGCTACTTCA+AGG - chr3.4:53128728-53128747 MS.gene69608:intron 35.0%
AAGAATTTCATCAGAGGCAA+TGG + chr3.4:53129381-53129400 None:intergenic 35.0%
AATCATCAACTTCCTGTTCT+GGG - chr3.4:53131894-53131913 MS.gene69608:CDS 35.0%
AATGAGGGAAGCTATTAAAC+AGG - chr3.4:53130282-53130301 MS.gene69608:CDS 35.0%
ACAATGCATTAGTAGGTGAA+AGG - chr3.4:53129339-53129358 MS.gene69608:CDS 35.0%
ACATGAACATGATGCAGAAT+TGG - chr3.4:53131549-53131568 MS.gene69608:intron 35.0%
AGTCCATAATTGGGGTAAAT+TGG + chr3.4:53131863-53131882 None:intergenic 35.0%
AGTGTTGTGAAACCATTGTT+AGG - chr3.4:53128492-53128511 MS.gene69608:CDS 35.0%
ATATAATGGTCTGTGAATGG+TGG + chr3.4:53130144-53130163 None:intergenic 35.0%
ATCTGTGTATTCTACTTCAC+TGG + chr3.4:53129207-53129226 None:intergenic 35.0%
ATGCGTGAAGTCATTAAAGA+CGG - chr3.4:53129896-53129915 MS.gene69608:CDS 35.0%
ATTTAGGTGGGGTTAATTCA+TGG + chr3.4:53129697-53129716 None:intergenic 35.0%
CAAGTGTGTAAATTTAGGTG+GGG + chr3.4:53129708-53129727 None:intergenic 35.0%
CACAATGAACCAAAACAATC+TGG + chr3.4:53128667-53128686 None:intergenic 35.0%
CACCCAGAAAATATACACAA+CGG + chr3.4:53130118-53130137 None:intergenic 35.0%
CTTGTGCTAACATTACCATT+AGG + chr3.4:53131710-53131729 None:intergenic 35.0%
GAATACGATGTCAACATTGA+TGG - chr3.4:53129983-53130002 MS.gene69608:CDS 35.0%
GATGAATCAGTTCAACAAGA+GGG - chr3.4:53129308-53129327 MS.gene69608:CDS 35.0%
GCAAGTGTGTAAATTTAGGT+GGG + chr3.4:53129709-53129728 None:intergenic 35.0%
GTACGTTACAAAGCCAAAAA+GGG - chr3.4:53129635-53129654 MS.gene69608:CDS 35.0%
GTAGATTTCATAGTTTGGCT+AGG + chr3.4:53129051-53129070 None:intergenic 35.0%
GTAGCATTCTTCTTCTTCTT+TGG + chr3.4:53131324-53131343 None:intergenic 35.0%
GTATTCTACTTCACTGGAAT+CGG + chr3.4:53129201-53129220 None:intergenic 35.0%
GTCCGAAATTGGTACTAATT+TGG - chr3.4:53128576-53128595 MS.gene69608:intron 35.0%
GTGATACTGAATACTCTACT+CGG - chr3.4:53131238-53131257 MS.gene69608:intron 35.0%
GTGTTGTGAAACCATTGTTA+GGG - chr3.4:53128493-53128512 MS.gene69608:CDS 35.0%
TAAATTGGCTGCATTTGCAT+TGG + chr3.4:53131848-53131867 None:intergenic 35.0%
TACGTTACAAAGCCAAAAAG+GGG - chr3.4:53129636-53129655 MS.gene69608:CDS 35.0%
TATAAATGGCAGCACACAAA+TGG + chr3.4:53128884-53128903 None:intergenic 35.0%
TATCGTTATGGAAGATGCAA+AGG - chr3.4:53129443-53129462 MS.gene69608:CDS 35.0%
TCTGAACAACGATTCTAATG+GGG - chr3.4:53131074-53131093 MS.gene69608:intron 35.0%
TCTTCCTAAGACAAGAAAGA+AGG + chr3.4:53128965-53128984 None:intergenic 35.0%
TCTTTCTTGTCTTAGGAAGA+TGG - chr3.4:53128965-53128984 MS.gene69608:CDS 35.0%
TGATGAATCAGTTCAACAAG+AGG - chr3.4:53129307-53129326 MS.gene69608:CDS 35.0%
TGCAAGTGTGTAAATTTAGG+TGG + chr3.4:53129710-53129729 None:intergenic 35.0%
TGGTGTATTTAGGGACATAA+TGG - chr3.4:53131185-53131204 MS.gene69608:intron 35.0%
TGTACGTTACAAAGCCAAAA+AGG - chr3.4:53129634-53129653 MS.gene69608:CDS 35.0%
TTGCGAAAATATCGTGAGAA+AGG - chr3.4:53130871-53130890 MS.gene69608:CDS 35.0%
TTTCATAGTTTGGCTAGGTA+TGG + chr3.4:53129046-53129065 None:intergenic 35.0%
! AAAGAAAACACCATTTCTGG+AGG - chr3.4:53130369-53130388 MS.gene69608:CDS 35.0%
! AAAGGTTTTGCCATTAGGAA+AGG - chr3.4:53129461-53129480 MS.gene69608:CDS 35.0%
! AAGAAAACACCATTTCTGGA+GGG - chr3.4:53130370-53130389 MS.gene69608:CDS 35.0%
! CAGAGATTTTACGATACAGT+GGG + chr3.4:53129733-53129752 None:intergenic 35.0%
! TCAGAGATTTTACGATACAG+TGG + chr3.4:53129734-53129753 None:intergenic 35.0%
! TTGGCACAAGTCATGAATTT+TGG - chr3.4:53131920-53131939 MS.gene69608:CDS 35.0%
! TTTTCGCAATGTGTAAACCA+TGG + chr3.4:53130860-53130879 None:intergenic 35.0%
! TTTTTGCAGGATACACAGAA+CGG - chr3.4:53131523-53131542 MS.gene69608:intron 35.0%
!! CAAAACAATCTGGCTTGTTT+GGG + chr3.4:53128657-53128676 None:intergenic 35.0%
!! CATCTGTTACAACTGCTTTT+GGG + chr3.4:53130252-53130271 None:intergenic 35.0%
!! TGATTGAAGAGCATCAAGTT+GGG + chr3.4:53129768-53129787 None:intergenic 35.0%
!!! ATTTTTTTGGGGTCCGAAAT+TGG - chr3.4:53128565-53128584 MS.gene69608:intron 35.0%
!!! TCGAAATTTTAGGGTTGTCA+TGG - chr3.4:53129151-53129170 MS.gene69608:CDS 35.0%
!!! TTTTTGTTTTGGCGACGTTT+TGG - chr3.4:53130054-53130073 MS.gene69608:CDS 35.0%
AACCCCAGCGTATATGTTAT+AGG + chr3.4:53128923-53128942 None:intergenic 40.0%
AAGACATATGTCAGAGCGAA+AGG - chr3.4:53130601-53130620 MS.gene69608:CDS 40.0%
AATGTTTGGTTATGACCCGT+TGG - chr3.4:53131028-53131047 MS.gene69608:CDS 40.0%
ACCCCAGCGTATATGTTATA+GGG + chr3.4:53128922-53128941 None:intergenic 40.0%
AGATGGAGATGGATCAATGA+GGG - chr3.4:53130267-53130286 MS.gene69608:CDS 40.0%
AGCACCTTCTTTCTTGTCTT+AGG - chr3.4:53128958-53128977 MS.gene69608:CDS 40.0%
AGCATATAACATTGGATCGG+TGG + chr3.4:53129963-53129982 None:intergenic 40.0%
AGTGTTAGAAAACGAACCTG+AGG + chr3.4:53128336-53128355 None:intergenic 40.0%
ATAAGCGATGAGATGCATCA+GGG + chr3.4:53130312-53130331 None:intergenic 40.0%
ATACGCGAACAAGTCATTGT+GGG - chr3.4:53130501-53130520 MS.gene69608:CDS 40.0%
ATTGGAGGCAACAATTACGT+TGG - chr3.4:53128828-53128847 MS.gene69608:CDS 40.0%
CAAAACTCCTCAAATTGCTC+CGG + chr3.4:53130427-53130446 None:intergenic 40.0%
CAACGATTCTAATGGGGAAA+TGG - chr3.4:53131080-53131099 MS.gene69608:intron 40.0%
CAACGCAACGCCAAATATAA+TGG + chr3.4:53130158-53130177 None:intergenic 40.0%
CAGCCAATTTACCCCAATTA+TGG - chr3.4:53131857-53131876 MS.gene69608:intron 40.0%
CCAAAAGCAGTTGTAACAGA+TGG - chr3.4:53130250-53130269 MS.gene69608:CDS 40.0%
CCACATCAAAACCCTAACAA+TGG + chr3.4:53128507-53128526 None:intergenic 40.0%
CCATTAGGAAAGGTGATGTT+AGG - chr3.4:53129471-53129490 MS.gene69608:CDS 40.0%
CCTAACATCACCTTTCCTAA+TGG + chr3.4:53129474-53129493 None:intergenic 40.0%
CTCAAGAGCAAAGAATGTGT+GGG - chr3.4:53130829-53130848 MS.gene69608:CDS 40.0%
CTTGATGCTCTTCAATCACA+TGG - chr3.4:53129770-53129789 MS.gene69608:CDS 40.0%
GAAGTACAAGTCATACTGCA+AGG - chr3.4:53131373-53131392 MS.gene69608:intron 40.0%
GAATCATCAACTTCCTGTTC+TGG - chr3.4:53131893-53131912 MS.gene69608:CDS 40.0%
GATAGCTTCACATTGAGATG+TGG + chr3.4:53130569-53130588 None:intergenic 40.0%
GATATGAATCTGTTGAGGGT+TGG - chr3.4:53128612-53128631 MS.gene69608:CDS 40.0%
GATCTCTGCTGTGTAAATCT+TGG + chr3.4:53130764-53130783 None:intergenic 40.0%
GATGCTGAAGATGATGTTGA+TGG - chr3.4:53129263-53129282 MS.gene69608:CDS 40.0%
GATGTTAGGAGCAATAGTAG+TGG - chr3.4:53129485-53129504 MS.gene69608:CDS 40.0%
GGAACACCACAAAGATTGTT+TGG - chr3.4:53129172-53129191 MS.gene69608:CDS 40.0%
GGATATATGGTTGCTCAGAA+GGG - chr3.4:53129815-53129834 MS.gene69608:CDS 40.0%
GGGTGCAAGTGTGTAAATTT+AGG + chr3.4:53129713-53129732 None:intergenic 40.0%
TACCCCAATTATGGACTTCA+TGG - chr3.4:53131866-53131885 MS.gene69608:intron 40.0%
TCAAGAGCAAAGAATGTGTG+GGG - chr3.4:53130830-53130849 MS.gene69608:CDS 40.0%
TCTCAAGAGCAAAGAATGTG+TGG - chr3.4:53130828-53130847 MS.gene69608:CDS 40.0%
TGAAATCTACGTCTTGTGCT+AGG - chr3.4:53129061-53129080 MS.gene69608:CDS 40.0%
TGCATTAGTAGGTGAAAGGT+TGG - chr3.4:53129343-53129362 MS.gene69608:CDS 40.0%
TTTCCAAAACCCTCCAGAAA+TGG + chr3.4:53130382-53130401 None:intergenic 40.0%
! CACTACATATCTCCCCTTTT+TGG + chr3.4:53129651-53129670 None:intergenic 40.0%
! CCAAACAAGCCAGATTGTTT+TGG - chr3.4:53128655-53128674 MS.gene69608:CDS 40.0%
! CCATCTGTTACAACTGCTTT+TGG + chr3.4:53130253-53130272 None:intergenic 40.0%
! CCTTGCTTCACAATTTGTTC+GGG + chr3.4:53129609-53129628 None:intergenic 40.0%
! CGGGTCATAACCAAACATTT+TGG + chr3.4:53131027-53131046 None:intergenic 40.0%
! CTTTTGCGTTCTTTGTCCAA+CGG + chr3.4:53131047-53131066 None:intergenic 40.0%
! GAGACATGAAGCACTTATCT+AGG - chr3.4:53129552-53129571 MS.gene69608:CDS 40.0%
! GATGCAAAGGTTTTGCCATT+AGG - chr3.4:53129456-53129475 MS.gene69608:CDS 40.0%
! GCACAAGTCATGAATTTTGG+TGG - chr3.4:53131923-53131942 MS.gene69608:CDS 40.0%
! GCCTTGCTTCACAATTTGTT+CGG + chr3.4:53129610-53129629 None:intergenic 40.0%
! GTAGACTATTTGAGACACGT+GGG - chr3.4:53130947-53130966 MS.gene69608:CDS 40.0%
! TGTAGACTATTTGAGACACG+TGG - chr3.4:53130946-53130965 MS.gene69608:CDS 40.0%
! TTTTACACCGGAGCAATTTG+AGG - chr3.4:53130417-53130436 MS.gene69608:CDS 40.0%
! TTTTGCGTTCTTTGTCCAAC+GGG + chr3.4:53131046-53131065 None:intergenic 40.0%
!! AAAAGTCTGTTTTGGGCTGA+TGG - chr3.4:53130013-53130032 MS.gene69608:CDS 40.0%
!! AAAGTCTGTTTTGGGCTGAT+GGG - chr3.4:53130014-53130033 MS.gene69608:CDS 40.0%
!! CCAAAACAATCTGGCTTGTT+TGG + chr3.4:53128658-53128677 None:intergenic 40.0%
!! CCATTGTTAGGGTTTTGATG+TGG - chr3.4:53128504-53128523 MS.gene69608:intron 40.0%
!! GTCATGAATTTTGGTGGTCA+TGG - chr3.4:53131929-53131948 MS.gene69608:CDS 40.0%
!! GTGATTGAAGAGCATCAAGT+TGG + chr3.4:53129769-53129788 None:intergenic 40.0%
!! TCATACTGCAAGGCATTGTT+CGG - chr3.4:53131383-53131402 MS.gene69608:intron 40.0%
!! TGAGAAAGGCATTGAAAGAG+AGG - chr3.4:53130885-53130904 MS.gene69608:CDS 40.0%
ACCGCCATGAAGTCCATAAT+TGG + chr3.4:53131873-53131892 None:intergenic 45.0%
CAAGAGCAAAGAATGTGTGG+GGG - chr3.4:53130831-53130850 MS.gene69608:CDS 45.0%
CAGATGGAGATGGATCAATG+AGG - chr3.4:53130266-53130285 MS.gene69608:CDS 45.0%
CATAAGCGATGAGATGCATC+AGG + chr3.4:53130313-53130332 None:intergenic 45.0%
CATACGCGAACAAGTCATTG+TGG - chr3.4:53130500-53130519 MS.gene69608:CDS 45.0%
CCAATCCCTGTGAAGAGTAA+AGG - chr3.4:53131294-53131313 MS.gene69608:intron 45.0%
CCCAATTATGGACTTCATGG+CGG - chr3.4:53131869-53131888 MS.gene69608:intron 45.0%
CCCGAACAAATTGTGAAGCA+AGG - chr3.4:53129606-53129625 MS.gene69608:CDS 45.0%
CCGCCATGAAGTCCATAATT+GGG + chr3.4:53131872-53131891 None:intergenic 45.0%
CCTTTACTCTTCACAGGGAT+TGG + chr3.4:53131297-53131316 None:intergenic 45.0%
CGCCATGAAGTCCATAATTG+GGG + chr3.4:53131871-53131890 None:intergenic 45.0%
GAGAGAGAAAGAAGAGGCAA+CGG + chr3.4:53128247-53128266 None:intergenic 45.0%
GAGCACCTTTACTCTTCACA+GGG + chr3.4:53131302-53131321 None:intergenic 45.0%
GAGTTTGTGTGCAACAAGCA+AGG - chr3.4:53129524-53129543 MS.gene69608:CDS 45.0%
GCAGTTGTAACAGATGGAGA+TGG - chr3.4:53130256-53130275 MS.gene69608:CDS 45.0%
GCATTGGATTAATAGCAGGC+TGG + chr3.4:53131832-53131851 None:intergenic 45.0%
GCCCTATAACATATACGCTG+GGG - chr3.4:53128918-53128937 MS.gene69608:CDS 45.0%
GGCCCTATAACATATACGCT+GGG - chr3.4:53128917-53128936 MS.gene69608:CDS 45.0%
GGGATATATGGTTGCTCAGA+AGG - chr3.4:53129814-53129833 MS.gene69608:CDS 45.0%
GGTTGAACTGCAGTTGTTAC+CGG + chr3.4:53131794-53131813 None:intergenic 45.0%
TAGAAAACGAACCTGAGGCT+TGG + chr3.4:53128331-53128350 None:intergenic 45.0%
TATATGGTTGCTCAGAAGGG+TGG - chr3.4:53129818-53129837 MS.gene69608:CDS 45.0%
TATATTTGGCGTTGCGTTGC+TGG - chr3.4:53130159-53130178 MS.gene69608:CDS 45.0%
TGGCCCTATAACATATACGC+TGG - chr3.4:53128916-53128935 MS.gene69608:CDS 45.0%
! CGGAGCAATTTGAGGAGTTT+TGG - chr3.4:53130425-53130444 MS.gene69608:CDS 45.0%
!! ACACCATTTCTGGAGGGTTT+TGG - chr3.4:53130376-53130395 MS.gene69608:CDS 45.0%
AAGGGTGGATACGCTGATGT+AGG - chr3.4:53129833-53129852 MS.gene69608:CDS 50.0%
AGAAAGAAGAGGCAACGGAG+TGG + chr3.4:53128242-53128261 None:intergenic 50.0%
AGAATGTGTGGGGGATACCA+TGG - chr3.4:53130840-53130859 MS.gene69608:CDS 50.0%
CATCACTCTCAATCGAACGC+AGG + chr3.4:53130738-53130757 None:intergenic 50.0%
CATCTCATCGCTTATGTGCC+TGG - chr3.4:53130317-53130336 MS.gene69608:CDS 50.0%
CGCATTCTTATGCAGATGCC+AGG + chr3.4:53130338-53130357 None:intergenic 50.0%
CGGCGTCCAAACAATCTTTG+TGG + chr3.4:53129181-53129200 None:intergenic 50.0%
GAAAGAAGAGGCAACGGAGT+GGG + chr3.4:53128241-53128260 None:intergenic 50.0%
GCACACGAGAGAGAAAGAAG+AGG + chr3.4:53128253-53128272 None:intergenic 50.0%
GGCTGGAACACATGAACAGT+AGG + chr3.4:53131815-53131834 None:intergenic 50.0%
TTTGGGCTGATGGGAGTAGT+AGG - chr3.4:53130023-53130042 MS.gene69608:CDS 50.0%
! AACTTCCTGTTCTGGGCTCT+TGG - chr3.4:53131901-53131920 MS.gene69608:CDS 50.0%
! GGAGCACCTTTACTCTTCAC+AGG + chr3.4:53131303-53131322 None:intergenic 50.0%
!! TTCGGAGACAGCTGATTTGC+CGG - chr3.4:53131772-53131791 MS.gene69608:intron 50.0%
ATTTGGCGTTGCGTTGCTGG+AGG - chr3.4:53130162-53130181 MS.gene69608:CDS 55.0%
TTGTGCCAAGAGCCCAGAAC+AGG + chr3.4:53131909-53131928 None:intergenic 55.0%
AACCTGAGGCTTGGAGAGCG+AGG + chr3.4:53128322-53128341 None:intergenic 60.0%
CGCAGGTGAGTCGTCAACAC+AGG + chr3.4:53130721-53130740 None:intergenic 60.0%
GGAGTGAAGATGGCGCTGCT+GGG + chr3.4:53128291-53128310 None:intergenic 60.0%
!! GGTGGTGGACGGAGTGAAGA+TGG + chr3.4:53128301-53128320 None:intergenic 60.0%
!! TTGGAGAGCGAGGTGGTGGA+CGG + chr3.4:53128312-53128331 None:intergenic 60.0%
AGGCTTGGAGAGCGAGGTGG+TGG + chr3.4:53128316-53128335 None:intergenic 65.0%
CACCTCGCTCTCCAAGCCTC+AGG - chr3.4:53128317-53128336 MS.gene69608:CDS 65.0%
CGGAGTGAAGATGGCGCTGC+TGG + chr3.4:53128292-53128311 None:intergenic 65.0%
CTGAGGCTTGGAGAGCGAGG+TGG + chr3.4:53128319-53128338 None:intergenic 65.0%
GGCGCTGCTGGGTCGCACGT+GGG + chr3.4:53128280-53128299 None:intergenic 75.0%
TGGCGCTGCTGGGTCGCACG+TGG + chr3.4:53128281-53128300 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 53128232 53131982 53128232 ID=MS.gene69608
chr3.4 mRNA 53128232 53131982 53128232 ID=MS.gene69608.t1;Parent=MS.gene69608
chr3.4 exon 53131876 53131982 53131876 ID=MS.gene69608.t1.exon1;Parent=MS.gene69608.t1
chr3.4 CDS 53131876 53131982 53131876 ID=cds.MS.gene69608.t1;Parent=MS.gene69608.t1
chr3.4 exon 53128810 53131051 53128810 ID=MS.gene69608.t1.exon2;Parent=MS.gene69608.t1
chr3.4 CDS 53128810 53131051 53128810 ID=cds.MS.gene69608.t1;Parent=MS.gene69608.t1
chr3.4 exon 53128602 53128682 53128602 ID=MS.gene69608.t1.exon3;Parent=MS.gene69608.t1
chr3.4 CDS 53128602 53128682 53128602 ID=cds.MS.gene69608.t1;Parent=MS.gene69608.t1
chr3.4 exon 53128232 53128522 53128232 ID=MS.gene69608.t1.exon4;Parent=MS.gene69608.t1
chr3.4 CDS 53128232 53128522 53128232 ID=cds.MS.gene69608.t1;Parent=MS.gene69608.t1
Gene Sequence

>MS.gene69608

ATGCGACCCACTCCGTTGCCTCTTCTTTCTCTCTCGTGTGCGCGACCCACGTGCGACCCAGCAGCGCCATCTTCACTCCGTCCACCACCTCGCTCTCCAAGCCTCAGGTTCGTTTTCTAACACTCGACAGAACAATCTGAATGAATGAATCTCCTTTTTCTTTTTAATTAGGGTTTTTTTGATTTTGGATTCTTTTATATGAATGTAACAGTGTTTAATTGGTTGATTGATTATGAAATTGGCATTATGAGGAATATATTAGTGTTGTGAAACCATTGTTAGGGTTTTGATGTGGCCAAAATTTGCGATTTTGTGATTATGCAATTGGAGATTATTTTTTTGGGGTCCGAAATTGGTACTAATTTGGTGTTATTGTTGTTGATATGAATCTGTTGAGGGTTGGTGTTTTTGCTCTTGATATGCCCAAACAAGCCAGATTGTTTTGGTTCATTGTGTAATTCAAGGACTGTTATTTCACTTTTCATATCAATCTTCTAACACAATTGAGCTACTTCAAGGATGTAGTGTAGTTATTAAAGCAGACACTAGGTTATAACAAAAAAAAGGTTTTCAACAATGTTATATACTTGTTTAGTATTGGAGGCAACAATTACGTTGGCATTCACTACATCTTATATTGTCTTTGTTTCCATTTGTGTGCTGCCATTTATATGTACATTTGGGTGGCCCTATAACATATACGCTGGGGTTTTTGTTTGTTTTCTTAGCACCTTCTTTCTTGTCTTAGGAAGATGGTTTTGCTTGCAATAATATATTGGCTATTTAAAAAAAAATATACATGAAATAGTTTCCATACCTAGCCAAACTATGAAATCTACGTCTTGTGCTAGGCTACATCTTTTGAAATAATATTATGCTGTTTTAAAAAAAAAAAAAAACTCATGCTTTGTTTAAATTTTCGAAATTTTAGGGTTGTCATGGAACACCACAAAGATTGTTTGGACGCCGATTCCAGTGAAGTAGAATACACAGATGATTTATATGATGATGAAGAACATCATAGTTCGTCTGATGCTGAAGATGATGTTGATGGTAATGCTGCTGATGATGTTCATGATGAATCAGTTCAACAAGAGGGTTCAATCAACAATGCATTAGTAGGTGAAAGGTTGGTGAATGTAAATTCCATTGCCTCTGATGAAATTCTTAAACTTGAATTTGGCACTGTTGATGAAGCATATGAATTTTATTATCGTTATGGAAGATGCAAAGGTTTTGCCATTAGGAAAGGTGATGTTAGGAGCAATAGTAGTGGAATAATTACAATGAGAGAGTTTGTGTGCAACAAGCAAGGCTTAAGAGACATGAAGCACTTATCTAGGAATGATAGAAAAAGAGATCATAGACGTATGACCCGAACAAATTGTGAAGCAAGGCTTCGTGTACGTTACAAAGCCAAAAAGGGGAGATATGTAGTGTCTAAATTTGAAGAGGGGCATAACCATGAATTAACCCCACCTAAATTTACACACTTGCACCCACTGTATCGTAAAATCTCTGAAGCAGATAGTGCCCAACTTGATGCTCTTCAATCACATGGAATCAGAACCTGTCATATAATGGGATATATGGTTGCTCAGAAGGGTGGATACGCTGATGTAGGATTTACTAAGAAAGATCTGTATAACTATTTTGATAAAAAGATGCGTGAAGTCATTAAAGACGGTGATGTTGCTGCTGCTCTAAAGCATCTTAATGTGAAGTCTTCCACCGATCCAATGTTATATGCTGAATACGATGTCAACATTGATGGAAGAATGAAAAGTCTGTTTTGGGCTGATGGGAGTAGTAGGTCTGATTATTTTTGTTTTGGCGACGTTTTGGCGTTTGACGCAACGTACAAGAAGAACAAATACAACTATCCGTTGTGTATATTTTCTGGGTGTAACCACCATTCACAGACCATTATATTTGGCGTTGCGTTGCTGGAGGATGAAACAATCGAGTCATATAAGTGGGTCTTGAACCGTTTTTTAGAATGTATGGAAAATAAATTCCCAAAAGCAGTTGTAACAGATGGAGATGGATCAATGAGGGAAGCTATTAAACAGGTATTCCCTGATGCATCTCATCGCTTATGTGCCTGGCATCTGCATAAGAATGCGCAAGAAAATGTAAAGAAAACACCATTTCTGGAGGGTTTTGGAAAAGCAATGTACTCAAATTTTACACCGGAGCAATTTGAGGAGTTTTGGTCAGAGTTAATTCAGAATAATGAACTTGAAGAAAATGATTGGGTTAAGAAGACATACGCGAACAAGTCATTGTGGGCAACCGCATATTTACGTGATAAGTTCTTTGGACGTATTAGAACCACATCTCAATGTGAAGCTATCAATGCAATTGTCAAGACATATGTCAGAGCGAAAGGCAACATTTTTGAATTTATGCATAATTTTGAACAGGTTTTGAGAGATTACAGAAATAGTGAGCTTGCTGCTGAGTTTAAATCAAAATTCACAGAACCTGTGTTGACGACTCACCTGCGTTCGATTGAGAGTGATGCTGCCAAGATTTACACAGCAGAGATCTTTAAAGAAGTTAAAGATGAAATTATTGAGGCTGGTGCAGTAAGTCTCAAGAGCAAAGAATGTGTGGGGGATACCATGGTTTACACATTGCGAAAATATCGTGAGAAAGGCATTGAAAGAGAGGTTGTATACGATGCTGCAAGTTTAGAATTTCAGTGTTCGTGTAGACTATTTGAGACACGTGGGATTCCATGTTGTCATGTTTTTTTTGTGATGAAGGAAGAAGACATTGATCACATACCAAAATGTTTGGTTATGACCCGTTGGACAAAGAACGCAAAAGATGCATTTCTGAACAACGATTCTAATGGGGAAATGGATTCTGATATGATTGAAGTTGCTAGATTTGGTGCTTACTGTTCTGCTTTTACTGCATTCTGTAAAGAAGCCTCAAAAAAGAATGGTGTATTTAGGGACATAATGGATGAAATTTTAAATCTTCAACAAAAGTATTGTGATACTGAATACTCTACTCGGTCTGAGAAATTAACAGATGATCAGGTAGGTGATCCAATCCCTGTGAAGAGTAAAGGTGCTCCAAAGAAGAAGAAGAATGCTACAAAAAGTGTCAGACACTGTTCAAAATGCAGAAGTACAAGTCATACTGCAAGGCATTGTTCGGTAATTTGTCTATATATGATCTGATGAATAATATTTTCACGAAATATGATTTATAATGTGCATGATAAGATATATATATATATATATATATATATATATATATATATAAGCAAGTTAATTTTTGCAGGATACACAGAACGGTGAACATGAACATGATGCAGAATTGGTATCTAACTCTATGGATGATTCTTTAAGCCAAAAAGAAAAGGTATGTTTGTTTGCTATGTTTTTACAATGCATCAGAATTACATCTTAAGAAAATGTGTATTAATTTATGTCATTCACAGAAAAATAAAAGGAAACCTAATGGTAATGTTAGCACAAGCAAGCAAAAGAAATATAAAGAGGCAGCACAATTTACAGAGATTTCGGAGACAGCTGATTTGCCGGTAACAACTGCAGTTCAACCTACTGTTCATGTGTTCCAGCCTGCTATTAATCCAATGCAAATGCAGCCAATTTACCCCAATTATGGACTTCATGGCGGTGAATCATCAACTTCCTGTTCTGGGCTCTTGGCACAAGTCATGAATTTTGGTGGTCATGGATTACATTTGAATAGTAATTTAGATAAATAA
Protein sequence

>MS.gene69608.t1

MRPTPLPLLSLSCARPTCDPAAPSSLRPPPRSPSLRVVMEHHKDCLDADSSEVEYTDDLYDDEEHHSSSDAEDDVDGNAADDVHDESVQQEGSINNALVGERLVNVNSIASDEILKLEFGTVDEAYEFYYRYGRCKGFAIRKGDVRSNSSGIITMREFVCNKQGLRDMKHLSRNDRKRDHRRMTRTNCEARLRVRYKAKKGRYVVSKFEEGHNHELTPPKFTHLHPLYRKISEADSAQLDALQSHGIRTCHIMGYMVAQKGGYADVGFTKKDLYNYFDKKMREVIKDGDVAAALKHLNVKSSTDPMLYAEYDVNIDGRMKSLFWADGSSRSDYFCFGDVLAFDATYKKNKYNYPLCIFSGCNHHSQTIIFGVALLEDETIESYKWVLNRFLECMENKFPKAVVTDGDGSMREAIKQVFPDASHRLCAWHLHKNAQENVKKTPFLEGFGKAMYSNFTPEQFEEFWSELIQNNELEENDWVKKTYANKSLWATAYLRDKFFGRIRTTSQCEAINAIVKTYVRAKGNIFEFMHNFEQVLRDYRNSELAAEFKSKFTEPVLTTHLRSIESDAAKIYTAEIFKEVKDEIIEAGAVSLKSKECVGDTMVYTLRKYREKGIEREVVYDAASLEFQCSCRLFETRGIPCCHVFFVMKEEDIDHIPKCLVMTRWTKNAKDAFLNNDSNGEMDSDMIEVARFGAYCSAFTAFCKEASKKNGVFRDIMDEILNLQQKYCDTEYSTRSEKLTDDQVGDPIPVKSKGAPKKKKNATKSVRHCSKCRSTSHTARHCSDTQNGEHEHDAELVSNSMDDSLSQKEKKNKRKPNGNVSTSKQKKYKEAAQFTEISETADLPVTTAVQPTVHVFQPAINPMQMQPIYPNYGLHGGESSTSCSGLLAQVMNFGGHGLHLNSNLDK