AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69699.t1 MTR_3g056110 96.679 542 18 0 1 542 1 542 0.0 1093
MS.gene69699.t1 MTR_5g094450 67.577 549 154 9 1 542 1 532 0.0 732
MS.gene69699.t1 MTR_5g097480 57.713 551 217 6 1 542 1 544 0.0 652
MS.gene69699.t1 MTR_4g133660 48.841 561 248 9 1 541 1 542 1.04e-178 514
MS.gene69699.t1 MTR_2g082090 58.871 372 153 0 170 541 207 578 6.89e-169 491
MS.gene69699.t1 MTR_2g026250 46.753 539 230 7 56 542 63 596 6.87e-166 484
MS.gene69699.t1 MTR_1g029420 50.000 370 185 0 173 542 223 592 3.62e-141 420
MS.gene69699.t1 MTR_3g089055 49.081 381 193 1 162 542 191 570 7.02e-138 411
MS.gene69699.t1 MTR_3g089055 49.081 381 193 1 162 542 186 565 1.34e-137 410
MS.gene69699.t1 MTR_7g027190 33.938 551 296 13 21 542 162 673 2.79e-78 259
MS.gene69699.t1 MTR_3g065980 35.476 389 216 11 172 542 169 540 7.91e-64 218
MS.gene69699.t1 MTR_4g064160 32.000 375 248 4 171 541 312 683 4.08e-62 216
MS.gene69699.t1 MTR_7g062120 30.319 376 255 4 171 542 368 740 6.89e-62 217
MS.gene69699.t1 MTR_4g064150 32.540 378 246 6 171 541 357 732 2.08e-61 215
MS.gene69699.t1 MTR_8g442410 32.552 384 238 7 171 542 151 525 3.09e-61 211
MS.gene69699.t1 MTR_2g097390 29.388 490 309 12 77 541 163 640 6.81e-61 212
MS.gene69699.t1 MTR_6g047750 37.464 347 188 8 210 542 331 662 1.94e-59 209
MS.gene69699.t1 MTR_2g097410 31.496 381 244 8 171 541 367 740 1.96e-59 210
MS.gene69699.t1 MTR_2g097350 29.191 507 310 14 61 541 156 639 2.13e-59 208
MS.gene69699.t1 MTR_7g057230 34.897 341 208 6 208 541 323 656 7.43e-59 207
MS.gene69699.t1 MTR_2g097310 29.613 439 284 7 122 541 205 637 1.02e-58 206
MS.gene69699.t1 MTR_4g064120 31.016 374 253 3 171 539 250 623 1.02e-57 203
MS.gene69699.t1 MTR_4g064180 31.635 373 250 3 171 538 250 622 2.20e-57 202
MS.gene69699.t1 MTR_7g074650 34.247 365 225 6 179 542 442 792 1.43e-56 203
MS.gene69699.t1 MTR_4g104020 29.688 384 248 5 172 542 146 520 1.72e-55 195
MS.gene69699.t1 MTR_4g064200 31.733 375 247 7 171 541 280 649 2.54e-55 197
MS.gene69699.t1 MTR_4g097080 29.825 399 245 11 163 542 121 503 3.01e-54 191
MS.gene69699.t1 MTR_2g097467 29.177 401 277 4 142 541 259 653 2.12e-53 192
MS.gene69699.t1 MTR_3g022830 27.578 417 246 5 176 542 26 436 4.99e-53 186
MS.gene69699.t1 MTR_4g077760 35.311 354 212 10 195 542 213 555 7.69e-51 183
MS.gene69699.t1 MTR_4g102790 31.122 392 236 13 169 541 285 661 5.87e-50 182
MS.gene69699.t1 MTR_2g097463 29.301 372 259 2 171 541 286 654 1.56e-49 181
MS.gene69699.t1 MTR_2g097473 29.759 373 256 5 171 541 285 653 3.49e-49 180
MS.gene69699.t1 MTR_2g097380 26.793 474 298 12 77 541 127 560 9.43e-48 175
MS.gene69699.t1 MTR_4g076020 32.432 370 216 10 179 542 88 429 1.57e-47 171
MS.gene69699.t1 MTR_5g015490 29.412 391 239 12 175 542 114 490 6.07e-47 171
MS.gene69699.t1 MTR_1g096030 28.993 407 229 12 172 542 77 459 1.63e-43 161
MS.gene69699.t1 MTR_1g069725 30.769 377 236 12 171 541 105 462 7.32e-43 159
MS.gene69699.t1 MTR_3g072710 30.376 372 222 10 194 541 141 499 3.50e-40 152
MS.gene69699.t1 MTR_5g058860 31.694 366 223 11 194 541 140 496 4.10e-40 152
MS.gene69699.t1 MTR_2g089100 25.118 422 272 9 154 541 31 442 1.15e-38 147
MS.gene69699.t1 MTR_2g099110 27.551 392 248 10 174 542 73 451 1.19e-35 139
MS.gene69699.t1 MTR_2g034250 27.506 389 254 9 172 542 207 585 7.76e-35 138
MS.gene69699.t1 MTR_5g015950 24.824 427 251 11 172 541 32 445 1.57e-32 130
MS.gene69699.t1 MTR_3g053270 24.824 427 251 11 172 541 32 445 1.57e-32 130
MS.gene69699.t1 MTR_4g095500 24.586 423 270 9 166 541 47 467 2.65e-32 129
MS.gene69699.t1 MTR_0092s0100 27.200 375 250 8 174 542 371 728 5.48e-32 131
MS.gene69699.t1 MTR_0092s0100 27.200 375 250 8 174 542 374 731 5.68e-32 131
MS.gene69699.t1 MTR_2g034280 26.706 337 232 6 172 499 200 530 5.46e-31 127
MS.gene69699.t1 MTR_4g026485 25.521 384 244 12 174 542 268 624 4.12e-30 125
MS.gene69699.t1 MTR_7g109580 24.672 381 267 9 171 542 185 554 8.48e-29 120
MS.gene69699.t1 MTR_2g034260 25.369 339 236 6 172 499 207 539 1.73e-28 120
MS.gene69699.t1 MTR_4g076140 30.045 223 148 3 160 380 32 248 3.86e-28 117
MS.gene69699.t1 MTR_4g076140 37.589 141 87 1 403 542 329 469 2.69e-26 112
MS.gene69699.t1 MTR_5g009080 28.774 212 144 2 172 382 53 258 6.94e-27 114
MS.gene69699.t1 MTR_5g009080 34.752 141 91 1 403 542 338 478 2.97e-23 103
MS.gene69699.t1 MTR_8g093070 28.212 397 246 17 160 541 123 495 1.18e-26 114
MS.gene69699.t1 MTR_1g086970 25.143 350 239 9 206 542 139 478 8.56e-26 110
MS.gene69699.t1 MTR_8g020840 25.310 403 245 15 173 542 169 548 6.77e-24 105
MS.gene69699.t1 MTR_8g077940 23.969 388 255 14 171 542 178 541 4.36e-17 84.7
MS.gene69699.t1 MTR_3g022580 30.894 123 84 1 420 542 62 183 1.18e-14 72.8
MS.gene69699.t1 MTR_3g022005 35.484 93 60 0 450 542 33 125 2.21e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69699.t1 AT1G50600 56.044 546 216 7 1 542 72 597 0.0 592
MS.gene69699.t1 AT1G50600 56.044 546 216 7 1 542 76 601 0.0 592
MS.gene69699.t1 AT1G50600 56.044 546 216 7 1 542 1 526 0.0 590
MS.gene69699.t1 AT5G48150 64.554 426 148 2 118 542 67 490 0.0 581
MS.gene69699.t1 AT5G48150 64.554 426 148 2 118 542 67 490 0.0 581
MS.gene69699.t1 AT5G48150 64.554 426 148 2 118 542 67 490 0.0 581
MS.gene69699.t1 AT5G48150 64.554 426 148 2 118 542 67 490 0.0 581
MS.gene69699.t1 AT2G04890 58.937 414 163 1 129 542 7 413 0.0 518
MS.gene69699.t1 AT4G17230 49.275 552 243 11 1 542 1 525 5.81e-171 494
MS.gene69699.t1 AT4G17230 49.275 552 243 11 1 542 1 525 5.81e-171 494
MS.gene69699.t1 AT1G21450 42.231 502 266 5 63 542 94 593 8.47e-136 407
MS.gene69699.t1 AT1G66350 36.267 375 215 9 172 542 152 506 1.20e-68 230
MS.gene69699.t1 AT5G17490 36.074 377 218 10 172 542 157 516 7.40e-68 228
MS.gene69699.t1 AT5G52510 38.218 348 190 7 210 542 303 640 1.54e-67 230
MS.gene69699.t1 AT1G07530 33.690 374 246 2 170 541 391 764 1.12e-66 230
MS.gene69699.t1 AT1G14920 35.433 381 216 10 172 542 169 529 2.27e-66 224
MS.gene69699.t1 AT3G03450 35.200 375 230 8 172 542 180 545 4.86e-65 221
MS.gene69699.t1 AT2G01570 35.904 376 221 9 172 542 221 581 2.61e-64 220
MS.gene69699.t1 AT2G29060 32.800 375 248 3 171 541 280 654 4.86e-63 218
MS.gene69699.t1 AT2G29060 32.800 375 248 3 171 541 317 691 7.13e-63 219
MS.gene69699.t1 AT5G59450 28.191 376 266 3 171 542 223 598 8.31e-55 195
MS.gene69699.t1 AT3G46600 30.108 372 258 2 172 542 1 371 1.12e-54 189
MS.gene69699.t1 AT3G46600 30.295 373 258 2 171 542 176 547 2.12e-54 192
MS.gene69699.t1 AT3G46600 30.295 373 258 2 171 542 208 579 2.18e-54 193
MS.gene69699.t1 AT5G66770 33.952 377 234 8 172 542 217 584 5.15e-54 192
MS.gene69699.t1 AT2G29065 31.332 383 248 8 171 541 248 627 1.76e-53 192
MS.gene69699.t1 AT3G54220 32.425 367 230 7 179 541 297 649 5.09e-53 191
MS.gene69699.t1 AT2G37650 28.989 376 258 5 171 542 331 701 1.05e-52 191
MS.gene69699.t1 AT2G37650 28.989 376 258 5 171 542 343 713 1.09e-52 191
MS.gene69699.t1 AT3G50650 34.986 363 218 7 189 542 189 542 3.32e-52 186
MS.gene69699.t1 AT1G50420 27.483 433 245 8 172 542 54 479 1.24e-51 184
MS.gene69699.t1 AT1G07520 29.676 401 269 7 150 541 132 528 8.21e-51 182
MS.gene69699.t1 AT1G07520 29.676 401 269 7 150 541 132 528 8.21e-51 182
MS.gene69699.t1 AT1G07520 29.676 401 269 7 150 541 296 692 2.81e-50 184
MS.gene69699.t1 AT1G07520 29.777 403 266 8 150 541 297 693 3.23e-50 184
MS.gene69699.t1 AT5G41920 32.637 383 224 11 172 542 40 400 1.25e-49 176
MS.gene69699.t1 AT4G37650 27.525 396 247 11 175 542 146 529 1.40e-44 165
MS.gene69699.t1 AT1G55580 28.019 414 246 9 172 542 41 445 1.64e-42 158
MS.gene69699.t1 AT1G63100 30.591 389 244 7 171 541 248 628 3.03e-42 160
MS.gene69699.t1 AT1G63100 30.591 389 244 7 171 541 273 653 3.32e-42 160
MS.gene69699.t1 AT3G49950 25.253 396 255 9 172 541 27 407 5.03e-36 139
MS.gene69699.t1 AT4G08250 29.412 306 200 6 244 541 179 476 3.12e-34 135
MS.gene69699.t1 AT3G60630 28.808 302 188 9 254 542 335 622 6.15e-26 112
MS.gene69699.t1 AT2G45160 25.562 356 217 13 207 542 312 639 1.04e-24 108
MS.gene69699.t1 AT4G00150 25.064 391 232 16 172 542 205 554 1.42e-23 105
MS.gene69699.t1 AT3G13840 23.725 451 294 14 125 542 77 510 1.09e-21 99.0
MS.gene69699.t1 AT4G36710 24.557 395 245 14 171 542 121 485 8.32e-20 92.8
MS.gene69699.t1 AT5G67411 29.082 196 122 6 295 479 9 198 1.53e-14 73.2

Find 116 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACCTGTTATTCTAATCTT+TGG 0.235841 3.4:+52885978 None:intergenic
CGCAAAATAGTATCCGTTTC+AGG 0.240168 3.4:-52886305 MS.gene69699:CDS
CTGCAAGAACGGAGCGTTAT+TGG 0.252017 3.4:+52885650 None:intergenic
GTGCACCATCTCTCTCTTCT+AGG 0.256808 3.4:+52885346 None:intergenic
ATCTGCTTCGTTCTTATTAG+AGG 0.270897 3.4:-52886453 MS.gene69699:CDS
GTTTCAAAGATTGCCTCTTC+TGG 0.289813 3.4:-52886239 MS.gene69699:CDS
CAGGCTCTTGCTCGCCGGCC+TGG 0.315935 3.4:-52886008 MS.gene69699:CDS
ATCTTATTGTTTCTTGTGCT+TGG 0.342571 3.4:-52885299 MS.gene69699:CDS
AGCAGATTCTTCTTGAACTT+TGG 0.342587 3.4:+52886469 None:intergenic
CCTCCAAAGATTAGAATAAC+AGG 0.365174 3.4:-52885981 MS.gene69699:CDS
GATGAGAAGGATAGAATGTT+TGG 0.365693 3.4:+52886765 None:intergenic
ATATGTCCGTACTTTAAATT+CGG 0.373248 3.4:-52886137 MS.gene69699:CDS
AGAGATGGTGCACTGTTTCT+TGG 0.374714 3.4:-52885336 MS.gene69699:CDS
CAGGCCGGCGAGCAAGAGCC+TGG 0.383296 3.4:+52886009 None:intergenic
CATTCACTTGAATTTGTCAA+TGG 0.402839 3.4:-52886614 MS.gene69699:CDS
TTCACCGCCACAGCTTCATT+AGG 0.419761 3.4:+52885801 None:intergenic
TAATGTCGAACAGCACTGTT+TGG 0.423158 3.4:-52885544 MS.gene69699:CDS
GTAGAACCTGGACTATTGTT+AGG 0.425781 3.4:+52886650 None:intergenic
CTTGATTGAAGAATTGATGA+AGG 0.426007 3.4:+52885401 None:intergenic
ATCCTCTCTTTGTGATCCCT+TGG 0.427208 3.4:+52885567 None:intergenic
AACATCAATTGATTCATAAA+CGG 0.428207 3.4:+52885596 None:intergenic
CAGAAACATGAAATTTCTTA+CGG 0.431673 3.4:-52886887 MS.gene69699:CDS
CCTGTTATTCTAATCTTTGG+AGG 0.435815 3.4:+52885981 None:intergenic
GCTCACTCATCTTATGCCTT+AGG 0.446845 3.4:+52886543 None:intergenic
TCATCGTATGCCCGCGGTGG+GGG 0.447317 3.4:-52885942 MS.gene69699:CDS
CAATTGATGTTGTTCTTCCA+AGG 0.452748 3.4:-52885584 MS.gene69699:CDS
TTCGGATATATGTCTGCAAA+TGG 0.452855 3.4:-52886119 MS.gene69699:CDS
GGCGAGCAAGAGCCTGGATT+AGG 0.464253 3.4:+52886015 None:intergenic
ATGAAGGAGCTCAATGGATA+AGG 0.465572 3.4:+52885417 None:intergenic
AGGCAGTCCAATCGAACGAT+TGG 0.470118 3.4:-52886285 MS.gene69699:CDS
TTGTCTAGATTCTCAATTGA+GGG 0.471983 3.4:+52886812 None:intergenic
TGCTTGTTGAAGCAGATTCA+AGG 0.473275 3.4:+52886720 None:intergenic
CTAATCTTTGGAGGTCCTCC+AGG 0.475160 3.4:+52885990 None:intergenic
TGAATGGTCACACTTTCATC+TGG 0.476627 3.4:+52885753 None:intergenic
TTCGATTGGACTGCCTGAAA+CGG 0.476643 3.4:+52886292 None:intergenic
TGGTACATGATGTAGCATAA+TGG 0.479324 3.4:+52885773 None:intergenic
AAATTTCTTACGGCTCTGAC+AGG 0.480661 3.4:-52886877 MS.gene69699:CDS
CAATTTGAAAATCAATTATG+TGG 0.486415 3.4:+52886060 None:intergenic
CTAACAGCATGGAACTCAAA+TGG 0.487453 3.4:+52885867 None:intergenic
TGTTCTAGAGTTTGATGAGA+AGG 0.490850 3.4:+52886752 None:intergenic
ATTCATCGTATGCCCGCGGT+GGG 0.494640 3.4:-52885944 MS.gene69699:CDS
GGTGAGAAACCGTGAGTAGA+GGG 0.496435 3.4:+52886671 None:intergenic
AAGTAAGAATCCTCCGCCTC+AGG 0.498080 3.4:+52886587 None:intergenic
ATCTCTAGCATCTCTACGTT+AGG 0.500658 3.4:+52886500 None:intergenic
ATGGTGCACTGTTTCTTGGT+TGG 0.503499 3.4:-52885332 MS.gene69699:CDS
CAATGGTTCACCTGAGGCGG+AGG 0.504414 3.4:-52886597 MS.gene69699:CDS
GTCACGAGCTTCTGAGTAAG+TGG 0.507829 3.4:-52885470 MS.gene69699:CDS
CAAAATTCTCCCTCTACTCA+CGG 0.508055 3.4:-52886680 MS.gene69699:CDS
TACGCTCCCAATCGTTCGAT+TGG 0.508907 3.4:+52886278 None:intergenic
TCCACTAGAGTCACCACCTT+AGG 0.511707 3.4:+52885687 None:intergenic
GGCAGTCCAATCGAACGATT+GGG 0.513943 3.4:-52886284 MS.gene69699:CDS
TCAGTGGGTGAGCCTAATCC+AGG 0.518047 3.4:-52886027 MS.gene69699:CDS
TGCACCATCTCTCTCTTCTA+GGG 0.519166 3.4:+52885347 None:intergenic
TCTTCGAGTCGCACCTCAGA+AGG 0.519582 3.4:+52885837 None:intergenic
TTCATCGTATGCCCGCGGTG+GGG 0.523230 3.4:-52885943 MS.gene69699:CDS
TTCTCACCTAACAATAGTCC+AGG 0.523328 3.4:-52886656 MS.gene69699:CDS
GTAGATTGTACTTCCAGAAG+AGG 0.526914 3.4:+52886226 None:intergenic
TTAAAATGCAGCGAGCCTAC+TGG 0.529023 3.4:-52886197 MS.gene69699:CDS
TCGACAAAACGCTGCAAGAA+CGG 0.530438 3.4:+52885639 None:intergenic
AATTGATGTTGTTCTTCCAA+GGG 0.531808 3.4:-52885583 MS.gene69699:CDS
ACATGGAGATGGTATCTAGA+GGG 0.537613 3.4:-52886412 MS.gene69699:CDS
TCTTATGCCTTAGGTCATCA+TGG 0.538147 3.4:+52886552 None:intergenic
ATTGTCTAGATTCTCAATTG+AGG 0.540884 3.4:+52886811 None:intergenic
TGTCAATGGTTCACCTGAGG+CGG 0.541775 3.4:-52886600 MS.gene69699:CDS
ATAGTCCAGGTTCTACGTTA+AGG 0.542222 3.4:-52886643 MS.gene69699:CDS
AGCCATCGCGGAAGCTATGA+AGG 0.542662 3.4:-52886096 MS.gene69699:CDS
TCGTGACAGGTTGCTGCGAT+TGG 0.544162 3.4:-52885724 MS.gene69699:CDS
GGCTAAGCACATGTCTCCTA+AGG 0.546864 3.4:-52885703 MS.gene69699:CDS
TAATCCAGGCTCTTGCTCGC+CGG 0.552248 3.4:-52886013 MS.gene69699:CDS
CTCCTTCATAGCTTCCGCGA+TGG 0.552554 3.4:+52886094 None:intergenic
CCATTCATAATCATCGTGAC+AGG 0.553760 3.4:-52885737 MS.gene69699:CDS
TGAACTTAGTAGCTTGCGAA+GGG 0.564647 3.4:-52885509 MS.gene69699:CDS
CCTGTCACGATGATTATGAA+TGG 0.565547 3.4:+52885737 None:intergenic
ATCATGACCATGATGACCTA+AGG 0.567502 3.4:-52886559 MS.gene69699:CDS
TTCATAAAGTACATGCATGT+AGG 0.567692 3.4:+52886160 None:intergenic
ACGATTGGGAGCGTACATGT+TGG 0.575556 3.4:-52886270 MS.gene69699:CDS
TCCTAAGGTGGTGACTCTAG+TGG 0.576537 3.4:-52885688 MS.gene69699:CDS
TATCGAGACAATTGAATGGA+TGG 0.577277 3.4:-52886339 MS.gene69699:CDS
AGGTGAGAAACCGTGAGTAG+AGG 0.577735 3.4:+52886670 None:intergenic
AAGGCATAAGATGAGTGAGC+TGG 0.578063 3.4:-52886540 MS.gene69699:CDS
ATGATATCGAGACAATTGAA+TGG 0.578703 3.4:-52886343 MS.gene69699:CDS
GTGAACTTAGTAGCTTGCGA+AGG 0.579196 3.4:-52885510 MS.gene69699:CDS
TCTTATTAGAGGCAGAGAAG+TGG 0.579922 3.4:-52886442 MS.gene69699:CDS
ATTGGGAGCGTACATGTTGG+AGG 0.587024 3.4:-52886267 MS.gene69699:CDS
TTATACTTGTGCAAAAGCTG+TGG 0.587474 3.4:-52886372 MS.gene69699:CDS
GCTCTTGCTCGCCGGCCTGG+AGG 0.588420 3.4:-52886005 MS.gene69699:CDS
TATTCATCGTATGCCCGCGG+TGG 0.589109 3.4:-52885945 MS.gene69699:CDS
TTCCAAGGGATCACAAAGAG+AGG 0.599204 3.4:-52885569 MS.gene69699:CDS
TGCGGCCTTAACGTAGAACC+TGG 0.605445 3.4:+52886638 None:intergenic
TACGACTCGAGATTCTGTAC+AGG 0.608834 3.4:+52886842 None:intergenic
TGTTAGAGTGTATCCTTCTG+AGG 0.611616 3.4:-52885850 MS.gene69699:CDS
AACATGGAGATGGTATCTAG+AGG 0.612916 3.4:-52886413 MS.gene69699:CDS
TCTTTGGAGGTCCTCCAGGC+CGG 0.613618 3.4:+52885994 None:intergenic
CTTCTAGGGTGTAATGTCCA+CGG 0.620198 3.4:+52885361 None:intergenic
AAGGATACACTCTAACAGCA+TGG 0.621994 3.4:+52885856 None:intergenic
GAATTGATGAAGGAGCTCAA+TGG 0.624450 3.4:+52885411 None:intergenic
AATCTTCTCGAGAGCTACCG+TGG 0.627998 3.4:-52885378 MS.gene69699:CDS
TCGTCCTAATGAAGCTGTGG+CGG 0.629870 3.4:-52885805 MS.gene69699:CDS
ATTTGTCAATGGTTCACCTG+AGG 0.630937 3.4:-52886603 MS.gene69699:CDS
ATATATCCGAATTTAAAGTA+CGG 0.633125 3.4:+52886131 None:intergenic
ATGACATGACTGCGCAAGCG+CGG 0.633313 3.4:+52885893 None:intergenic
ACAAATTCAAGTGAATGTTG+CGG 0.633662 3.4:+52886620 None:intergenic
CATGGAGATGGTATCTAGAG+GGG 0.636479 3.4:-52886411 MS.gene69699:CDS
TGTTTGGCAGCTGTTATCAG+AGG 0.643018 3.4:+52886781 None:intergenic
GAAAGCAGTTCATTACCAGT+AGG 0.643103 3.4:+52886182 None:intergenic
AATTGGACAAGGAACTCAGT+GGG 0.647751 3.4:-52886042 MS.gene69699:CDS
TAAGCACATGTCTCCTAAGG+TGG 0.649063 3.4:-52885700 MS.gene69699:CDS
ACTTCGTCCTAATGAAGCTG+TGG 0.652136 3.4:-52885808 MS.gene69699:CDS
TTGCGAAGGGGAAGAAAGAG+TGG 0.653404 3.4:-52885496 MS.gene69699:CDS
GTCTGCAAATGGAGCCATCG+CGG 0.659747 3.4:-52886108 MS.gene69699:CDS
GAGCTGGAATCTGTTATGCG+AGG 0.671920 3.4:-52886524 MS.gene69699:CDS
GAACTTAGTAGCTTGCGAAG+GGG 0.697810 3.4:-52885508 MS.gene69699:CDS
CATCGCGGAAGCTATGAAGG+AGG 0.707945 3.4:-52886093 MS.gene69699:CDS
TCTTATTCATCGTATGCCCG+CGG 0.710163 3.4:-52885948 MS.gene69699:CDS
TACACCCTAGAAGAGAGAGA+TGG 0.711736 3.4:-52885351 MS.gene69699:CDS
AAATTGGACAAGGAACTCAG+TGG 0.723091 3.4:-52886043 MS.gene69699:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTGAAAATCAATTATG+TGG + chr3.4:52886135-52886154 None:intergenic 20.0%
!!! AACATCAATTGATTCATAAA+CGG + chr3.4:52886599-52886618 None:intergenic 20.0%
!!! ATATATCCGAATTTAAAGTA+CGG + chr3.4:52886064-52886083 None:intergenic 20.0%
!!! CACATAATTGATTTTCAAAT+TGG - chr3.4:52886133-52886152 MS.gene69699:CDS 20.0%
! ATACCTGTTATTCTAATCTT+TGG + chr3.4:52886217-52886236 None:intergenic 25.0%
! ATATGTCCGTACTTTAAATT+CGG - chr3.4:52886055-52886074 MS.gene69699:CDS 25.0%
! CAGAAACATGAAATTTCTTA+CGG - chr3.4:52885305-52885324 MS.gene69699:CDS 25.0%
!!! ATTGATTTTCAAATTGGACA+AGG - chr3.4:52886139-52886158 MS.gene69699:CDS 25.0%
AATTGATGTTGTTCTTCCAA+GGG - chr3.4:52886609-52886628 MS.gene69699:CDS 30.0%
ACAAATTCAAGTGAATGTTG+CGG + chr3.4:52885575-52885594 None:intergenic 30.0%
ATGATATCGAGACAATTGAA+TGG - chr3.4:52885849-52885868 MS.gene69699:CDS 30.0%
ATTGTCTAGATTCTCAATTG+AGG + chr3.4:52885384-52885403 None:intergenic 30.0%
CATTCACTTGAATTTGTCAA+TGG - chr3.4:52885578-52885597 MS.gene69699:CDS 30.0%
CTTGATTGAAGAATTGATGA+AGG + chr3.4:52886794-52886813 None:intergenic 30.0%
TTCATAAAGTACATGCATGT+AGG + chr3.4:52886035-52886054 None:intergenic 30.0%
TTGTCTAGATTCTCAATTGA+GGG + chr3.4:52885383-52885402 None:intergenic 30.0%
! ATCTTATTGTTTCTTGTGCT+TGG - chr3.4:52886893-52886912 MS.gene69699:CDS 30.0%
AGAGAAGTGGAAGAAAAACA+TGG - chr3.4:52885763-52885782 MS.gene69699:CDS 35.0%
AGCAGATTCTTCTTGAACTT+TGG + chr3.4:52885726-52885745 None:intergenic 35.0%
ATCTGCTTCGTTCTTATTAG+AGG - chr3.4:52885739-52885758 MS.gene69699:CDS 35.0%
CAATTGATGTTGTTCTTCCA+AGG - chr3.4:52886608-52886627 MS.gene69699:CDS 35.0%
CCTCCAAAGATTAGAATAAC+AGG - chr3.4:52886211-52886230 MS.gene69699:CDS 35.0%
CCTGTTATTCTAATCTTTGG+AGG + chr3.4:52886214-52886233 None:intergenic 35.0%
GATGAGAAGGATAGAATGTT+TGG + chr3.4:52885430-52885449 None:intergenic 35.0%
TATCGAGACAATTGAATGGA+TGG - chr3.4:52885853-52885872 MS.gene69699:CDS 35.0%
TGGTACATGATGTAGCATAA+TGG + chr3.4:52886422-52886441 None:intergenic 35.0%
TTATACTTGTGCAAAAGCTG+TGG - chr3.4:52885820-52885839 MS.gene69699:CDS 35.0%
TTCGGATATATGTCTGCAAA+TGG - chr3.4:52886073-52886092 MS.gene69699:CDS 35.0%
!! GTAGAGGGAGAATTTTGATA+AGG + chr3.4:52885509-52885528 None:intergenic 35.0%
!! TGTTCTAGAGTTTGATGAGA+AGG + chr3.4:52885443-52885462 None:intergenic 35.0%
AAATTGGACAAGGAACTCAG+TGG - chr3.4:52886149-52886168 MS.gene69699:CDS 40.0%
AACATGGAGATGGTATCTAG+AGG - chr3.4:52885779-52885798 MS.gene69699:CDS 40.0%
AAGGATACACTCTAACAGCA+TGG + chr3.4:52886339-52886358 None:intergenic 40.0%
AATTGGACAAGGAACTCAGT+GGG - chr3.4:52886150-52886169 MS.gene69699:CDS 40.0%
ACATGGAGATGGTATCTAGA+GGG - chr3.4:52885780-52885799 MS.gene69699:CDS 40.0%
ATAGTCCAGGTTCTACGTTA+AGG - chr3.4:52885549-52885568 MS.gene69699:CDS 40.0%
ATCATGACCATGATGACCTA+AGG - chr3.4:52885633-52885652 MS.gene69699:CDS 40.0%
ATCTCTAGCATCTCTACGTT+AGG + chr3.4:52885695-52885714 None:intergenic 40.0%
ATGAAGGAGCTCAATGGATA+AGG + chr3.4:52886778-52886797 None:intergenic 40.0%
ATTTGTCAATGGTTCACCTG+AGG - chr3.4:52885589-52885608 MS.gene69699:CDS 40.0%
CAAAATTCTCCCTCTACTCA+CGG - chr3.4:52885512-52885531 MS.gene69699:CDS 40.0%
CCATTCATAATCATCGTGAC+AGG - chr3.4:52886455-52886474 MS.gene69699:CDS 40.0%
CCTGTCACGATGATTATGAA+TGG + chr3.4:52886458-52886477 None:intergenic 40.0%
CGCAAAATAGTATCCGTTTC+AGG - chr3.4:52885887-52885906 MS.gene69699:CDS 40.0%
CTAACAGCATGGAACTCAAA+TGG + chr3.4:52886328-52886347 None:intergenic 40.0%
GAAAGCAGTTCATTACCAGT+AGG + chr3.4:52886013-52886032 None:intergenic 40.0%
GAATTGATGAAGGAGCTCAA+TGG + chr3.4:52886784-52886803 None:intergenic 40.0%
GTAGAACCTGGACTATTGTT+AGG + chr3.4:52885545-52885564 None:intergenic 40.0%
GTAGATTGTACTTCCAGAAG+AGG + chr3.4:52885969-52885988 None:intergenic 40.0%
GTGGAAGAAAAACATGGAGA+TGG - chr3.4:52885769-52885788 MS.gene69699:CDS 40.0%
GTTTCAAAGATTGCCTCTTC+TGG - chr3.4:52885953-52885972 MS.gene69699:CDS 40.0%
TAATGTCGAACAGCACTGTT+TGG - chr3.4:52886648-52886667 MS.gene69699:CDS 40.0%
TCTTATGCCTTAGGTCATCA+TGG + chr3.4:52885643-52885662 None:intergenic 40.0%
TCTTATTAGAGGCAGAGAAG+TGG - chr3.4:52885750-52885769 MS.gene69699:CDS 40.0%
TGAATGGTCACACTTTCATC+TGG + chr3.4:52886442-52886461 None:intergenic 40.0%
TGTTAGAGTGTATCCTTCTG+AGG - chr3.4:52886342-52886361 MS.gene69699:CDS 40.0%
TTCTCACCTAACAATAGTCC+AGG - chr3.4:52885536-52885555 MS.gene69699:CDS 40.0%
! AAATTTCTTACGGCTCTGAC+AGG - chr3.4:52885315-52885334 MS.gene69699:CDS 40.0%
! TGAACTTAGTAGCTTGCGAA+GGG - chr3.4:52886683-52886702 MS.gene69699:CDS 40.0%
! TGCTTGTTGAAGCAGATTCA+AGG + chr3.4:52885475-52885494 None:intergenic 40.0%
AAGGCATAAGATGAGTGAGC+TGG - chr3.4:52885652-52885671 MS.gene69699:CDS 45.0%
ACTTCGTCCTAATGAAGCTG+TGG - chr3.4:52886384-52886403 MS.gene69699:CDS 45.0%
ATCCTCTCTTTGTGATCCCT+TGG + chr3.4:52886628-52886647 None:intergenic 45.0%
CATGGAGATGGTATCTAGAG+GGG - chr3.4:52885781-52885800 MS.gene69699:CDS 45.0%
GCTCACTCATCTTATGCCTT+AGG + chr3.4:52885652-52885671 None:intergenic 45.0%
TAAGCACATGTCTCCTAAGG+TGG - chr3.4:52886492-52886511 MS.gene69699:CDS 45.0%
TACACCCTAGAAGAGAGAGA+TGG - chr3.4:52886841-52886860 MS.gene69699:CDS 45.0%
TACGACTCGAGATTCTGTAC+AGG + chr3.4:52885353-52885372 None:intergenic 45.0%
TCGACAAAACGCTGCAAGAA+CGG + chr3.4:52886556-52886575 None:intergenic 45.0%
TCTTATTCATCGTATGCCCG+CGG - chr3.4:52886244-52886263 MS.gene69699:CDS 45.0%
TGCACCATCTCTCTCTTCTA+GGG + chr3.4:52886848-52886867 None:intergenic 45.0%
TGTTTGGCAGCTGTTATCAG+AGG + chr3.4:52885414-52885433 None:intergenic 45.0%
TTAAAATGCAGCGAGCCTAC+TGG - chr3.4:52885995-52886014 MS.gene69699:CDS 45.0%
TTCCAAGGGATCACAAAGAG+AGG - chr3.4:52886623-52886642 MS.gene69699:CDS 45.0%
TTCGATTGGACTGCCTGAAA+CGG + chr3.4:52885903-52885922 None:intergenic 45.0%
! AGAGATGGTGCACTGTTTCT+TGG - chr3.4:52886856-52886875 MS.gene69699:CDS 45.0%
! ATGGTGCACTGTTTCTTGGT+TGG - chr3.4:52886860-52886879 MS.gene69699:CDS 45.0%
! GAACTTAGTAGCTTGCGAAG+GGG - chr3.4:52886684-52886703 MS.gene69699:CDS 45.0%
! GTGAACTTAGTAGCTTGCGA+AGG - chr3.4:52886682-52886701 MS.gene69699:CDS 45.0%
!! CTTCTAGGGTGTAATGTCCA+CGG + chr3.4:52886834-52886853 None:intergenic 45.0%
!!! AAAAAGCGTTTTGCGATGGC+TGG - chr3.4:52886745-52886764 MS.gene69699:CDS 45.0%
!!! GTGGAAAAAGCGTTTTGCGA+TGG - chr3.4:52886741-52886760 MS.gene69699:CDS 45.0%
AAGTAAGAATCCTCCGCCTC+AGG + chr3.4:52885608-52885627 None:intergenic 50.0%
AATCTTCTCGAGAGCTACCG+TGG - chr3.4:52886814-52886833 MS.gene69699:CDS 50.0%
AGGCAGTCCAATCGAACGAT+TGG - chr3.4:52885907-52885926 MS.gene69699:CDS 50.0%
AGGTGAGAAACCGTGAGTAG+AGG + chr3.4:52885525-52885544 None:intergenic 50.0%
GAGCTGGAATCTGTTATGCG+AGG - chr3.4:52885668-52885687 MS.gene69699:CDS 50.0%
GGCAGTCCAATCGAACGATT+GGG - chr3.4:52885908-52885927 MS.gene69699:CDS 50.0%
GGCTAAGCACATGTCTCCTA+AGG - chr3.4:52886489-52886508 MS.gene69699:CDS 50.0%
GGTGAGAAACCGTGAGTAGA+GGG + chr3.4:52885524-52885543 None:intergenic 50.0%
GTCACGAGCTTCTGAGTAAG+TGG - chr3.4:52886722-52886741 MS.gene69699:CDS 50.0%
GTGCACCATCTCTCTCTTCT+AGG + chr3.4:52886849-52886868 None:intergenic 50.0%
TACGCTCCCAATCGTTCGAT+TGG + chr3.4:52885917-52885936 None:intergenic 50.0%
TCCACTAGAGTCACCACCTT+AGG + chr3.4:52886508-52886527 None:intergenic 50.0%
TCCTAAGGTGGTGACTCTAG+TGG - chr3.4:52886504-52886523 MS.gene69699:CDS 50.0%
TCGTCCTAATGAAGCTGTGG+CGG - chr3.4:52886387-52886406 MS.gene69699:CDS 50.0%
TGTCAATGGTTCACCTGAGG+CGG - chr3.4:52885592-52885611 MS.gene69699:CDS 50.0%
TTCACCGCCACAGCTTCATT+AGG + chr3.4:52886394-52886413 None:intergenic 50.0%
TTGCGAAGGGGAAGAAAGAG+TGG - chr3.4:52886696-52886715 MS.gene69699:CDS 50.0%
! ACGATTGGGAGCGTACATGT+TGG - chr3.4:52885922-52885941 MS.gene69699:CDS 50.0%
! ATTGGGAGCGTACATGTTGG+AGG - chr3.4:52885925-52885944 MS.gene69699:CDS 50.0%
! CTAATCTTTGGAGGTCCTCC+AGG + chr3.4:52886205-52886224 None:intergenic 50.0%
! CTGCAAGAACGGAGCGTTAT+TGG + chr3.4:52886545-52886564 None:intergenic 50.0%
!! GTGGGGGCGTTGATATAGTA+GGG - chr3.4:52886266-52886285 MS.gene69699:CDS 50.0%
!! TGGGGGCGTTGATATAGTAG+GGG - chr3.4:52886267-52886286 MS.gene69699:CDS 50.0%
AGCCATCGCGGAAGCTATGA+AGG - chr3.4:52886096-52886115 MS.gene69699:CDS 55.0%
ATGACATGACTGCGCAAGCG+CGG + chr3.4:52886302-52886321 None:intergenic 55.0%
ATTCATCGTATGCCCGCGGT+GGG - chr3.4:52886248-52886267 MS.gene69699:CDS 55.0%
CATCGCGGAAGCTATGAAGG+AGG - chr3.4:52886099-52886118 MS.gene69699:CDS 55.0%
CTCCTTCATAGCTTCCGCGA+TGG + chr3.4:52886101-52886120 None:intergenic 55.0%
GTCTGCAAATGGAGCCATCG+CGG - chr3.4:52886084-52886103 MS.gene69699:CDS 55.0%
TAATCCAGGCTCTTGCTCGC+CGG - chr3.4:52886179-52886198 MS.gene69699:CDS 55.0%
TATTCATCGTATGCCCGCGG+TGG - chr3.4:52886247-52886266 MS.gene69699:CDS 55.0%
TCAGTGGGTGAGCCTAATCC+AGG - chr3.4:52886165-52886184 MS.gene69699:CDS 55.0%
TCGTGACAGGTTGCTGCGAT+TGG - chr3.4:52886468-52886487 MS.gene69699:CDS 55.0%
TCTTCGAGTCGCACCTCAGA+AGG + chr3.4:52886358-52886377 None:intergenic 55.0%
TGCGGCCTTAACGTAGAACC+TGG + chr3.4:52885557-52885576 None:intergenic 55.0%
!! GGTGGGGGCGTTGATATAGT+AGG - chr3.4:52886265-52886284 MS.gene69699:CDS 55.0%
CAATGGTTCACCTGAGGCGG+AGG - chr3.4:52885595-52885614 MS.gene69699:CDS 60.0%
CTATATCAACGCCCCCACCG+CGG + chr3.4:52886264-52886283 None:intergenic 60.0%
GGCGAGCAAGAGCCTGGATT+AGG + chr3.4:52886180-52886199 None:intergenic 60.0%
TATATCAACGCCCCCACCGC+GGG + chr3.4:52886263-52886282 None:intergenic 60.0%
TTCATCGTATGCCCGCGGTG+GGG - chr3.4:52886249-52886268 MS.gene69699:CDS 60.0%
! TCTTTGGAGGTCCTCCAGGC+CGG + chr3.4:52886201-52886220 None:intergenic 60.0%
TCATCGTATGCCCGCGGTGG+GGG - chr3.4:52886250-52886269 MS.gene69699:CDS 65.0%
CAGGCCGGCGAGCAAGAGCC+TGG + chr3.4:52886186-52886205 None:intergenic 75.0%
CAGGCTCTTGCTCGCCGGCC+TGG - chr3.4:52886184-52886203 MS.gene69699:CDS 75.0%
GCTCTTGCTCGCCGGCCTGG+AGG - chr3.4:52886187-52886206 MS.gene69699:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 52885293 52886921 52885293 ID=MS.gene69699
chr3.4 mRNA 52885293 52886921 52885293 ID=MS.gene69699.t1;Parent=MS.gene69699
chr3.4 exon 52885293 52886921 52885293 ID=MS.gene69699.t1.exon1;Parent=MS.gene69699.t1
chr3.4 CDS 52885293 52886921 52885293 ID=cds.MS.gene69699.t1;Parent=MS.gene69699.t1
Gene Sequence

>MS.gene69699

ATGCAAACATCTCAGAAACATGAAATTTCTTACGGCTCTGACAGGTTCTACGTTGAGCCTGTACAGAATCTCGAGTCGTATTGCTTACCCTCAATTGAGAATCTAGACAATTATTCTTCCTCTGATAACAGCTGCCAAACATTCTATCCTTCTCATCAAACTCTAGAACAATACAACAACCTTGAATCTGCTTCAACAAGCAACAACAGTTTACCTTATCAAAATTCTCCCTCTACTCACGGTTTCTCACCTAACAATAGTCCAGGTTCTACGTTAAGGCCGCAACATTCACTTGAATTTGTCAATGGTTCACCTGAGGCGGAGGATTCTTACTTGACATATCATGACCATGATGACCTAAGGCATAAGATGAGTGAGCTGGAATCTGTTATGCGAGGACCTAACGTAGAGATGCTAGAGATGTATGATACCAAAGTTCAAGAAGAATCTGCTTCGTTCTTATTAGAGGCAGAGAAGTGGAAGAAAAACATGGAGATGGTATCTAGAGGGGATTTGAAAGAGATGCTTTATACTTGTGCAAAAGCTGTGGATGTGAATGATATCGAGACAATTGAATGGATGGTAACTGAGTTACGCAAAATAGTATCCGTTTCAGGCAGTCCAATCGAACGATTGGGAGCGTACATGTTGGAGGCACTTGTTTCAAAGATTGCCTCTTCTGGAAGTACAATCTACAAATCGTTAAAATGCAGCGAGCCTACTGGTAATGAACTGCTTTCCTACATGCATGTACTTTATGAAATATGTCCGTACTTTAAATTCGGATATATGTCTGCAAATGGAGCCATCGCGGAAGCTATGAAGGAGGAAAATGAAGTCCACATAATTGATTTTCAAATTGGACAAGGAACTCAGTGGGTGAGCCTAATCCAGGCTCTTGCTCGCCGGCCTGGAGGACCTCCAAAGATTAGAATAACAGGTATTGATGACTCTTATTCATCGTATGCCCGCGGTGGGGGCGTTGATATAGTAGGGGAGAAGTTATCCGCGCTTGCGCAGTCATGTCATGTACCATTTGAGTTCCATGCTGTTAGAGTGTATCCTTCTGAGGTGCGACTCGAAGACTTTGAACTTCGTCCTAATGAAGCTGTGGCGGTGAACTTTGCCATTATGCTACATCATGTACCAGATGAAAGTGTGACCATTCATAATCATCGTGACAGGTTGCTGCGATTGGCTAAGCACATGTCTCCTAAGGTGGTGACTCTAGTGGAGCAAGAATTCAACACCAATAACGCTCCGTTCTTGCAGCGTTTTGTCGAGACAATGAACTACTACTCAGCCGTTTATGAATCAATTGATGTTGTTCTTCCAAGGGATCACAAAGAGAGGATTAATGTCGAACAGCACTGTTTGGCTCGTGAAGTTGTGAACTTAGTAGCTTGCGAAGGGGAAGAAAGAGTGGAACGTCACGAGCTTCTGAGTAAGTGGAAAAAGCGTTTTGCGATGGCTGGATTTACGCCTTATCCATTGAGCTCCTTCATCAATTCTTCAATCAAGAATCTTCTCGAGAGCTACCGTGGACATTACACCCTAGAAGAGAGAGATGGTGCACTGTTTCTTGGTTGGATGAATCAAGATCTTATTGTTTCTTGTGCTTGGAGATGA
Protein sequence

>MS.gene69699.t1

MQTSQKHEISYGSDRFYVEPVQNLESYCLPSIENLDNYSSSDNSCQTFYPSHQTLEQYNNLESASTSNNSLPYQNSPSTHGFSPNNSPGSTLRPQHSLEFVNGSPEAEDSYLTYHDHDDLRHKMSELESVMRGPNVEMLEMYDTKVQEESASFLLEAEKWKKNMEMVSRGDLKEMLYTCAKAVDVNDIETIEWMVTELRKIVSVSGSPIERLGAYMLEALVSKIASSGSTIYKSLKCSEPTGNELLSYMHVLYEICPYFKFGYMSANGAIAEAMKEENEVHIIDFQIGQGTQWVSLIQALARRPGGPPKIRITGIDDSYSSYARGGGVDIVGEKLSALAQSCHVPFEFHAVRVYPSEVRLEDFELRPNEAVAVNFAIMLHHVPDESVTIHNHRDRLLRLAKHMSPKVVTLVEQEFNTNNAPFLQRFVETMNYYSAVYESIDVVLPRDHKERINVEQHCLAREVVNLVACEGEERVERHELLSKWKKRFAMAGFTPYPLSSFINSSIKNLLESYRGHYTLEERDGALFLGWMNQDLIVSCAWR