Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69714.t1 | RHN76102.1 | 89 | 91 | 10 | 0 | 5 | 95 | 264 | 354 | 1.90E-35 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69714.t1 | Q9XE58 | 53.4 | 88 | 39 | 1 | 8 | 93 | 362 | 449 | 1.4e-18 | 94.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69714.t1 | G7IRV1 | 89.0 | 91 | 10 | 0 | 5 | 95 | 336 | 426 | 1.4e-35 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene69714.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69714.t1 | MTR_2g097410 | 89.011 | 91 | 10 | 0 | 5 | 95 | 336 | 426 | 2.81e-48 | 166 |
| MS.gene69714.t1 | MTR_4g064150 | 60.494 | 81 | 32 | 0 | 19 | 99 | 340 | 420 | 1.12e-28 | 111 |
| MS.gene69714.t1 | MTR_3g087300 | 89.091 | 55 | 6 | 0 | 5 | 59 | 154 | 208 | 1.25e-26 | 100 |
| MS.gene69714.t1 | MTR_4g064160 | 58.824 | 85 | 35 | 0 | 9 | 93 | 285 | 369 | 5.10e-26 | 103 |
| MS.gene69714.t1 | MTR_4g064120 | 59.494 | 79 | 32 | 0 | 21 | 99 | 235 | 313 | 5.94e-26 | 103 |
| MS.gene69714.t1 | MTR_7g062120 | 47.573 | 103 | 44 | 1 | 22 | 124 | 354 | 446 | 4.37e-24 | 98.6 |
| MS.gene69714.t1 | MTR_4g064180 | 61.429 | 70 | 27 | 0 | 21 | 90 | 235 | 304 | 8.86e-24 | 97.4 |
| MS.gene69714.t1 | MTR_2g097390 | 57.143 | 70 | 30 | 0 | 25 | 94 | 260 | 329 | 1.06e-22 | 94.4 |
| MS.gene69714.t1 | MTR_2g097350 | 56.522 | 69 | 30 | 0 | 26 | 94 | 260 | 328 | 3.93e-22 | 92.8 |
| MS.gene69714.t1 | MTR_2g097310 | 58.824 | 68 | 25 | 1 | 30 | 94 | 259 | 326 | 5.31e-21 | 89.7 |
| MS.gene69714.t1 | MTR_2g097463 | 65.517 | 58 | 20 | 0 | 34 | 91 | 284 | 341 | 1.69e-18 | 82.4 |
| MS.gene69714.t1 | MTR_4g064200 | 63.636 | 55 | 20 | 0 | 35 | 89 | 279 | 333 | 4.62e-17 | 78.2 |
| MS.gene69714.t1 | MTR_2g097467 | 51.282 | 78 | 36 | 1 | 16 | 91 | 263 | 340 | 8.63e-17 | 77.4 |
| MS.gene69714.t1 | MTR_2g097473 | 61.404 | 57 | 22 | 0 | 35 | 91 | 284 | 340 | 2.37e-16 | 76.3 |
| MS.gene69714.t1 | MTR_2g097380 | 55.556 | 45 | 20 | 0 | 45 | 89 | 229 | 273 | 2.63e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69714.t1 | AT1G07530 | 53.409 | 88 | 39 | 1 | 8 | 93 | 362 | 449 | 3.07e-24 | 99.0 |
| MS.gene69714.t1 | AT2G37650 | 42.017 | 119 | 57 | 2 | 28 | 144 | 323 | 431 | 1.97e-20 | 87.8 |
| MS.gene69714.t1 | AT2G37650 | 42.017 | 119 | 57 | 2 | 28 | 144 | 335 | 443 | 1.99e-20 | 87.8 |
| MS.gene69714.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 281 | 371 | 2.67e-20 | 87.4 |
| MS.gene69714.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 280 | 370 | 2.86e-20 | 87.4 |
| MS.gene69714.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 116 | 206 | 3.66e-20 | 87.0 |
| MS.gene69714.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 116 | 206 | 3.66e-20 | 87.0 |
| MS.gene69714.t1 | AT2G29060 | 52.703 | 74 | 35 | 0 | 20 | 93 | 264 | 337 | 1.69e-19 | 85.1 |
| MS.gene69714.t1 | AT2G29060 | 52.703 | 74 | 35 | 0 | 20 | 93 | 301 | 374 | 1.71e-19 | 85.1 |
| MS.gene69714.t1 | AT4G39630 | 49.398 | 83 | 38 | 2 | 94 | 172 | 147 | 229 | 2.55e-17 | 76.6 |
| MS.gene69714.t1 | AT4G39630 | 49.383 | 81 | 37 | 2 | 96 | 172 | 136 | 216 | 4.17e-17 | 75.9 |
| MS.gene69714.t1 | AT2G29065 | 61.017 | 59 | 23 | 0 | 34 | 92 | 246 | 304 | 4.50e-16 | 75.5 |
| MS.gene69714.t1 | AT3G46600 | 40.708 | 113 | 56 | 3 | 6 | 111 | 149 | 257 | 3.37e-15 | 72.8 |
| MS.gene69714.t1 | AT3G46600 | 41.346 | 104 | 51 | 2 | 6 | 102 | 181 | 281 | 3.87e-15 | 72.8 |
| MS.gene69714.t1 | AT5G59450 | 49.275 | 69 | 35 | 0 | 23 | 91 | 210 | 278 | 6.57e-15 | 72.0 |
| MS.gene69714.t1 | AT3G46600 | 46.575 | 73 | 32 | 1 | 37 | 102 | 1 | 73 | 1.17e-12 | 65.5 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTATTTAAATTTCTACA+TGG | + | 56880:9970-9989 | MS.gene69714:intron | 15.0% |
| !! | TTAATTAATCATACCATTTA+TGG | - | 56880:10743-10762 | MS.gene69714:intergenic | 15.0% |
| !! | AGATTATCCATGAATTTAAA+AGG | + | 56880:10371-10390 | MS.gene69714:intron | 20.0% |
| !! | TGATTTAATGTCTATTGTAT+AGG | + | 56880:10414-10433 | MS.gene69714:intron | 20.0% |
| !!! | AACATTTCCTTTTAAATTCA+TGG | - | 56880:10381-10400 | MS.gene69714:intergenic | 20.0% |
| !!! | TTACTTTTTGTGACAAAAAT+AGG | + | 56880:10283-10302 | MS.gene69714:intron | 20.0% |
| ! | AAGCATATTTATATACCTCA+TGG | + | 56880:10151-10170 | MS.gene69714:intron | 25.0% |
| ! | AGTTCATTTAAATGAAATGC+AGG | - | 56880:10517-10536 | MS.gene69714:intergenic | 25.0% |
| ! | GTACAGAAATTTGAATTCAT+TGG | + | 56880:9407-9426 | MS.gene69714:intron | 25.0% |
| ! | GTCTTATAAGCTAACTTTAT+TGG | + | 56880:9908-9927 | MS.gene69714:intron | 25.0% |
| ! | TATGTAATATTTAAGCCATG+AGG | - | 56880:10169-10188 | MS.gene69714:intergenic | 25.0% |
| !! | TTTTCTTTTTGTAAGTTGCA+TGG | - | 56880:9433-9452 | MS.gene69714:intergenic | 25.0% |
| !!! | GATATGTTTTTGAGTGTAAA+AGG | + | 56880:10539-10558 | MS.gene69714:intron | 25.0% |
| !!! | TTTGTGATTTTGATTGCAAA+AGG | + | 56880:9813-9832 | MS.gene69714:intron | 25.0% |
| AAAATAGGTTGTGAAACATG+AGG | + | 56880:10298-10317 | MS.gene69714:intron | 30.0% | |
| AAATAGGTTGTGAAACATGA+GGG | + | 56880:10299-10318 | MS.gene69714:intron | 30.0% | |
| AATAACATGGAAGCCATAAA+TGG | + | 56880:10727-10746 | MS.gene69714:CDS | 30.0% | |
| AATGTCTATTGTATAGGCTT+TGG | + | 56880:10420-10439 | MS.gene69714:intron | 30.0% | |
| ATGAATTGTTGAAGCAGATT+AGG | + | 56880:9635-9654 | MS.gene69714:CDS | 30.0% | |
| CATTTAAATGAAATGCAGGA+AGG | - | 56880:10513-10532 | MS.gene69714:intergenic | 30.0% | |
| TGTTTACAAGGACAATAACA+TGG | + | 56880:10714-10733 | MS.gene69714:CDS | 30.0% | |
| ! | AATCAGCACTAAACATCTTT+TGG | - | 56880:9774-9793 | MS.gene69714:intergenic | 30.0% |
| !! | CAGTTACTTTCATTTTGACA+GGG | + | 56880:10015-10034 | MS.gene69714:intron | 30.0% |
| !! | TCAGTTACTTTCATTTTGAC+AGG | + | 56880:10014-10033 | MS.gene69714:intron | 30.0% |
| !! | TGTTGATAATCTGTGTTTCA+AGG | + | 56880:10055-10074 | MS.gene69714:CDS | 30.0% |
| ACCTTTCATCATCATAGAGA+TGG | - | 56880:9878-9897 | MS.gene69714:intergenic | 35.0% | |
| ATTGTCCTTGTAAACACCTA+GGG | - | 56880:10710-10729 | MS.gene69714:intergenic | 35.0% | |
| GATCATGTTACAGAAACGAA+CGG | - | 56880:10350-10369 | MS.gene69714:intergenic | 35.0% | |
| GCTAACATCTAAGTTTCAGA+TGG | - | 56880:10451-10470 | MS.gene69714:intergenic | 35.0% | |
| GTGTAAAAGGTTGTCACAAA+TGG | + | 56880:10552-10571 | MS.gene69714:intron | 35.0% | |
| TATTGTCCTTGTAAACACCT+AGG | - | 56880:10711-10730 | MS.gene69714:intergenic | 35.0% | |
| ! | TCCATCTCTATGATGATGAA+AGG | + | 56880:9874-9893 | MS.gene69714:intron | 35.0% |
| !! | TAGTTAGCGTTGATAATCGT+AGG | + | 56880:10255-10274 | MS.gene69714:intron | 35.0% |
| !!! | AGTGCTGATTTTGATTGTGT+AGG | + | 56880:9784-9803 | MS.gene69714:intron | 35.0% |
| ATACCTTTGGATCACTTGCA+AGG | + | 56880:10119-10138 | MS.gene69714:intron | 40.0% | |
| CTGTGTCTGCTAATGACAAT+AGG | + | 56880:9605-9624 | MS.gene69714:CDS | 40.0% | |
| GCTGTTAACTCTTCAGTTGA+AGG | - | 56880:10578-10597 | MS.gene69714:intergenic | 40.0% | |
| TAGATGTTAGCGATGCAAGA+AGG | + | 56880:10460-10479 | MS.gene69714:intron | 40.0% | |
| TATCCTTGCAAGTGATCCAA+AGG | - | 56880:10125-10144 | MS.gene69714:intergenic | 40.0% | |
| TGAACAAAAGGATGGATCAG+TGG | + | 56880:9468-9487 | MS.gene69714:intron | 40.0% | |
| TTGTCCTTGTAAACACCTAG+GGG | - | 56880:10709-10728 | MS.gene69714:intergenic | 40.0% | |
| TTGTGCACACAGAACCAAAA+GGG | - | 56880:9584-9603 | MS.gene69714:intergenic | 40.0% | |
| TTGTGTGCTGAACAAAAGGA+TGG | + | 56880:9460-9479 | MS.gene69714:intron | 40.0% | |
| ! | AGTAGTCAGAGGTTTTCTGT+TGG | + | 56880:10619-10638 | MS.gene69714:CDS | 40.0% |
| ! | GTAGTCAGAGGTTTTCTGTT+GGG | + | 56880:10620-10639 | MS.gene69714:CDS | 40.0% |
| ! | GTCTATTGTATAGGCTTTGG+AGG | + | 56880:10423-10442 | MS.gene69714:intron | 40.0% |
| ! | TCCTTTTGTTCAGCACACAA+CGG | - | 56880:9460-9479 | MS.gene69714:intergenic | 40.0% |
| !! | ACACAGGTTGGTAAACTTGA+TGG | + | 56880:9502-9521 | MS.gene69714:CDS | 40.0% |
| !! | AGGTTGGTAAACTTGATGGA+GGG | + | 56880:9506-9525 | MS.gene69714:CDS | 40.0% |
| AAGGCGTGTTCGAAGAAACA+AGG | + | 56880:9529-9548 | MS.gene69714:CDS | 45.0% | |
| ACAAAAGGATGGATCAGTGG+TGG | + | 56880:9471-9490 | MS.gene69714:intron | 45.0% | |
| AGAGGGATGTTGTGGATTTG+AGG | + | 56880:9557-9576 | MS.gene69714:CDS | 45.0% | |
| AGGCGTGTTCGAAGAAACAA+GGG | + | 56880:9530-9549 | MS.gene69714:CDS | 45.0% | |
| AGGTGATGCATCACAGAGAA+CGG | + | 56880:9675-9694 | MS.gene69714:CDS | 45.0% | |
| ATCACCCCTAGGTGTTTACA+AGG | + | 56880:10702-10721 | MS.gene69714:CDS | 45.0% | |
| CTGCTACACTCACATACCTT+TGG | + | 56880:10106-10125 | MS.gene69714:intron | 45.0% | |
| CTTGTGCACACAGAACCAAA+AGG | - | 56880:9585-9604 | MS.gene69714:intergenic | 45.0% | |
| GAAGAAACAAGGGAGGAAGA+GGG | + | 56880:9540-9559 | MS.gene69714:CDS | 45.0% | |
| GAAGGAGAGATACTTACACC+AGG | - | 56880:10495-10514 | MS.gene69714:intergenic | 45.0% | |
| GAGATGCTTCTTTCTGTCCA+TGG | + | 56880:10679-10698 | MS.gene69714:CDS | 45.0% | |
| GATGACACCCAAAACACTTC+GGG | + | 56880:10642-10661 | MS.gene69714:CDS | 45.0% | |
| GGATGACACCCAAAACACTT+CGG | + | 56880:10641-10660 | MS.gene69714:CDS | 45.0% | |
| TGGTAAACTTGATGGAGGGA+AGG | + | 56880:9510-9529 | MS.gene69714:CDS | 45.0% | |
| ! | ACAGGGAGAACAAGCTTTGT+TGG | + | 56880:10032-10051 | MS.gene69714:intron | 45.0% |
| ! | ACTTTATTGGCTGCAAGCTC+AGG | + | 56880:9921-9940 | MS.gene69714:intron | 45.0% |
| ! | AGTTACTTTCTTGCCTCAGG+GGG | + | 56880:9837-9856 | MS.gene69714:intron | 45.0% |
| ! | CAAAGGTATGTGAGTGTAGC+AGG | - | 56880:10108-10127 | MS.gene69714:intergenic | 45.0% |
| ! | CAGCTGAATATGCGTTTTGC+AGG | + | 56880:10594-10613 | MS.gene69714:intron | 45.0% |
| ! | CAGTTACTTTCTTGCCTCAG+GGG | + | 56880:9836-9855 | MS.gene69714:intron | 45.0% |
| ! | CTTTATTGGCTGCAAGCTCA+GGG | + | 56880:9922-9941 | MS.gene69714:intron | 45.0% |
| ! | GCAGTTACTTTCTTGCCTCA+GGG | + | 56880:9835-9854 | MS.gene69714:intron | 45.0% |
| ! | TGTGGATTTGAGGACCCTTT+TGG | + | 56880:9567-9586 | MS.gene69714:CDS | 45.0% |
| ! | TTTTGCAGGTGAGTAGTCAG+AGG | + | 56880:10608-10627 | MS.gene69714:intron | 45.0% |
| !! | ACAGAAAGAAGCATCTCTCC+AGG | - | 56880:10676-10695 | MS.gene69714:intergenic | 45.0% |
| !! | AGGAAGCATTCTTGTCCTTC+AGG | + | 56880:9655-9674 | MS.gene69714:CDS | 45.0% |
| !! | CAGGTTGGTAAACTTGATGG+AGG | + | 56880:9505-9524 | MS.gene69714:CDS | 45.0% |
| !! | TTGGGAACCCGAAGTGTTTT+GGG | - | 56880:10652-10671 | MS.gene69714:intergenic | 45.0% |
| AAACACCTAGGGGTGATCCA+TGG | - | 56880:10699-10718 | MS.gene69714:intergenic | 50.0% | |
| CGAAGAAACAAGGGAGGAAG+AGG | + | 56880:9539-9558 | MS.gene69714:CDS | 50.0% | |
| CGTGTTCGAAGAAACAAGGG+AGG | + | 56880:9533-9552 | MS.gene69714:CDS | 50.0% | |
| CTCTGTGATGCATCACCTGA+AGG | - | 56880:9673-9692 | MS.gene69714:intergenic | 50.0% | |
| GCCGTTGTGTGCTGAACAAA+AGG | + | 56880:9456-9475 | MS.gene69714:intron | 50.0% | |
| GTGGAGAGTAGAACACAGGT+TGG | + | 56880:9490-9509 | MS.gene69714:CDS | 50.0% | |
| TAATGCGATTGAAGCGCGCA+TGG | + | 56880:9711-9730 | MS.gene69714:CDS | 50.0% | |
| ! | AGTGGTGGAGAGTAGAACAC+AGG | + | 56880:9486-9505 | MS.gene69714:CDS | 50.0% |
| ! | GGCAGTTACTTTCTTGCCTC+AGG | + | 56880:9834-9853 | MS.gene69714:intron | 50.0% |
| !! | AAGAAGCATCTCTCCAGGCT+TGG | - | 56880:10671-10690 | MS.gene69714:intergenic | 50.0% |
| !! | AGAAGCATCTCTCCAGGCTT+GGG | - | 56880:10670-10689 | MS.gene69714:intergenic | 50.0% |
| !! | CTTGGGAACCCGAAGTGTTT+TGG | - | 56880:10653-10672 | MS.gene69714:intergenic | 50.0% |
| AGGGAGGAAGAGGGATGTTG+TGG | + | 56880:9549-9568 | MS.gene69714:CDS | 55.0% | |
| CATACAATCTCGTCCCCCTG+AGG | - | 56880:9853-9872 | MS.gene69714:intergenic | 55.0% | |
| GCAAGAAGGTCTCTGACTCC+TGG | + | 56880:10474-10493 | MS.gene69714:intron | 55.0% | |
| TCTGTCCATGGATCACCCCT+AGG | + | 56880:10691-10710 | MS.gene69714:CDS | 55.0% | |
| !! | CGCATGGTTGGTGCTGGTAA+CGG | + | 56880:9727-9746 | MS.gene69714:CDS | 55.0% |
| GCGATTGAAGCGCGCATGGT+TGG | + | 56880:9715-9734 | MS.gene69714:CDS | 60.0% | |
| ! | ACACTTCGGGTTCCCAAGCC+TGG | + | 56880:10655-10674 | MS.gene69714:CDS | 60.0% |
| ! | GAAGCGCGCATGGTTGGTGC+TGG | + | 56880:9721-9740 | MS.gene69714:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56880 | gene | 9391 | 10762 | 9391 | ID=MS.gene69714 |
| 56880 | mRNA | 9391 | 10762 | 9391 | ID=MS.gene69714.t1;Parent=MS.gene69714 |
| 56880 | exon | 9391 | 9402 | 9391 | ID=MS.gene69714.t1.exon1;Parent=MS.gene69714.t1 |
| 56880 | CDS | 9391 | 9402 | 9391 | ID=cds.MS.gene69714.t1;Parent=MS.gene69714.t1 |
| 56880 | exon | 9478 | 9748 | 9478 | ID=MS.gene69714.t1.exon2;Parent=MS.gene69714.t1 |
| 56880 | CDS | 9478 | 9748 | 9478 | ID=cds.MS.gene69714.t1;Parent=MS.gene69714.t1 |
| 56880 | exon | 10036 | 10082 | 10036 | ID=MS.gene69714.t1.exon3;Parent=MS.gene69714.t1 |
| 56880 | CDS | 10036 | 10082 | 10036 | ID=cds.MS.gene69714.t1;Parent=MS.gene69714.t1 |
| 56880 | exon | 10305 | 10358 | 10305 | ID=MS.gene69714.t1.exon4;Parent=MS.gene69714.t1 |
| 56880 | CDS | 10305 | 10358 | 10305 | ID=cds.MS.gene69714.t1;Parent=MS.gene69714.t1 |
| 56880 | exon | 10616 | 10762 | 10616 | ID=MS.gene69714.t1.exon5;Parent=MS.gene69714.t1 |
| 56880 | CDS | 10616 | 10762 | 10616 | ID=cds.MS.gene69714.t1;Parent=MS.gene69714.t1 |
| Gene Sequence |
| Protein sequence |
