Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69761.t1 | XP_024634747.1 | 91.6 | 500 | 40 | 2 | 1 | 499 | 36 | 534 | 2.60E-255 | 891 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69761.t1 | Q9SFD5 | 50.4 | 508 | 224 | 7 | 7 | 496 | 47 | 544 | 2.7e-131 | 470.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69761.t1 | A0A072V1B9 | 88.1 | 503 | 43 | 4 | 1 | 499 | 24 | 513 | 1.0e-240 | 842.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene69761.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69761.t1 | MTR_3g081360 | 92.247 | 503 | 22 | 4 | 1 | 499 | 24 | 513 | 0.0 | 937 |
| MS.gene69761.t1 | MTR_3g082790 | 79.198 | 524 | 82 | 5 | 1 | 498 | 41 | 563 | 0.0 | 817 |
| MS.gene69761.t1 | MTR_3g082790 | 79.198 | 524 | 82 | 5 | 1 | 498 | 1 | 523 | 0.0 | 815 |
| MS.gene69761.t1 | MTR_2g026310 | 49.123 | 513 | 240 | 8 | 2 | 496 | 39 | 548 | 1.92e-163 | 474 |
| MS.gene69761.t1 | MTR_7g063290 | 46.830 | 489 | 212 | 7 | 7 | 495 | 43 | 483 | 1.24e-142 | 419 |
| MS.gene69761.t1 | MTR_2g026380 | 45.253 | 495 | 220 | 8 | 7 | 496 | 44 | 492 | 5.81e-138 | 407 |
| MS.gene69761.t1 | MTR_3g081380 | 77.852 | 149 | 31 | 1 | 1 | 147 | 35 | 183 | 1.63e-82 | 254 |
| MS.gene69761.t1 | MTR_3g081420 | 37.500 | 208 | 121 | 2 | 295 | 496 | 2 | 206 | 8.13e-41 | 146 |
| MS.gene69761.t1 | MTR_3g081390 | 78.889 | 90 | 19 | 0 | 53 | 142 | 36 | 125 | 2.66e-40 | 142 |
| MS.gene69761.t1 | MTR_7g062030 | 69.811 | 53 | 16 | 0 | 7 | 59 | 57 | 109 | 1.64e-22 | 93.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69761.t1 | AT3G07740 | 50.870 | 517 | 223 | 8 | 1 | 496 | 17 | 523 | 1.15e-166 | 481 |
| MS.gene69761.t1 | AT3G07740 | 50.969 | 516 | 222 | 8 | 2 | 496 | 39 | 544 | 3.82e-166 | 481 |
| MS.gene69761.t1 | AT3G07740 | 50.287 | 523 | 222 | 9 | 2 | 496 | 39 | 551 | 1.60e-163 | 474 |
| MS.gene69761.t1 | AT4G16420 | 50.612 | 490 | 188 | 8 | 11 | 498 | 45 | 482 | 6.69e-163 | 470 |
| MS.gene69761.t1 | AT4G16420 | 49.796 | 490 | 196 | 7 | 11 | 498 | 45 | 486 | 5.32e-162 | 468 |
| MS.gene69761.t1 | AT4G16420 | 48.577 | 492 | 198 | 6 | 11 | 498 | 45 | 485 | 7.82e-161 | 465 |
| MS.gene69761.t1 | AT3G07740 | 50.830 | 482 | 209 | 7 | 33 | 496 | 2 | 473 | 2.50e-153 | 446 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAAGATTGACAGAATTTA+TGG | 0.116257 | 3.4:+77334917 | MS.gene69761:CDS |
| TTTGGCAGAAATGGAATTTA+AGG | 0.163949 | 3.4:+77333129 | MS.gene69761:CDS |
| GTAGGATTTGTCTTATTCTA+TGG | 0.220086 | 3.4:+77330400 | MS.gene69761:intron |
| CTGACTCATGGAAGCCTTCT+TGG | 0.224645 | 3.4:-77331688 | None:intergenic |
| CAAAAGAATAAAAGACTTAA+AGG | 0.230732 | 3.4:+77333735 | MS.gene69761:CDS |
| GAAAAGAAACCTAAATTATC+TGG | 0.246092 | 3.4:+77333016 | MS.gene69761:CDS |
| TATGGTTTGCTTGTGGAAAA+AGG | 0.250512 | 3.4:+77334935 | MS.gene69761:CDS |
| GCCATTGACATGCATGGATT+TGG | 0.256378 | 3.4:+77330191 | MS.gene69761:CDS |
| TTATATTCCCCAGATAATTT+AGG | 0.264509 | 3.4:-77333025 | None:intergenic |
| TGCATAGAGTGCTTCTTTGT+TGG | 0.282569 | 3.4:+77328110 | MS.gene69761:CDS |
| TGGATATGCATCAGATTTCT+TGG | 0.286020 | 3.4:-77334876 | None:intergenic |
| TCACATATTTGAAGCTCTTC+AGG | 0.296633 | 3.4:-77333628 | None:intergenic |
| ACATGCATGGATTTGGGAAT+TGG | 0.309773 | 3.4:+77330198 | MS.gene69761:CDS |
| CTCTGCCTTCTTAGATTCTT+CGG | 0.316463 | 3.4:-77331510 | None:intergenic |
| CAATGAGGCCGAGCAAGTTT+TGG | 0.325682 | 3.4:+77333111 | MS.gene69761:CDS |
| TTTAATACCATAACTCGATA+TGG | 0.332731 | 3.4:-77328164 | None:intergenic |
| CCATTGACATGCATGGATTT+GGG | 0.362070 | 3.4:+77330192 | MS.gene69761:CDS |
| TCTTCACCTGCACTCCAATC+TGG | 0.373150 | 3.4:-77330017 | None:intergenic |
| TGTCTTTGTGATAGCTTGTA+TGG | 0.382689 | 3.4:-77334535 | None:intergenic |
| AGAGTCAGCTCTGCATTTGA+AGG | 0.397453 | 3.4:-77331379 | None:intergenic |
| TTGCAGGAAGCTAGAATTGC+TGG | 0.400705 | 3.4:+77334369 | MS.gene69761:intron |
| TATGGCTGGCGGTGCATCCT+TGG | 0.406764 | 3.4:-77334517 | None:intergenic |
| GGTGAGAGAAATTTCTCAAA+AGG | 0.408187 | 3.4:-77333607 | None:intergenic |
| ATCAATTTATTGAACAAAAG+AGG | 0.414539 | 3.4:+77334415 | MS.gene69761:CDS |
| CCTCTCAACACTTCCATAGA+TGG | 0.419056 | 3.4:-77333049 | None:intergenic |
| TCAGAATAATGATGGAGTTA+AGG | 0.422803 | 3.4:+77331707 | MS.gene69761:CDS |
| CATTTCTGCCAAAACTTGCT+CGG | 0.427096 | 3.4:-77333119 | None:intergenic |
| TATAAAGAAAGTGGTCAGAT+TGG | 0.427378 | 3.4:+77334462 | MS.gene69761:CDS |
| GAAGCCTTCTTGGTTATACT+TGG | 0.427656 | 3.4:-77331678 | None:intergenic |
| TGGGGAATATAAGCCATCTA+TGG | 0.429785 | 3.4:+77333036 | MS.gene69761:CDS |
| AGAAATTTCTCAAAAGGATC+AGG | 0.437392 | 3.4:-77333601 | None:intergenic |
| TAAATTCTGTCAATCTTGCT+TGG | 0.438413 | 3.4:-77334914 | None:intergenic |
| CACAAAGACACTAGAAGAAT+GGG | 0.442742 | 3.4:+77334548 | MS.gene69761:CDS |
| CTGCAGGTGCTCCGTGGCTA+CGG | 0.453584 | 3.4:+77333184 | MS.gene69761:CDS |
| TAGGATTTGTCTTATTCTAT+GGG | 0.455897 | 3.4:+77330401 | MS.gene69761:intron |
| TTTCAAGCAGGTTGGAGCAC+TGG | 0.458498 | 3.4:+77332992 | MS.gene69761:CDS |
| TGAAGAGAAGTTACTTATTG+AGG | 0.462048 | 3.4:+77330034 | MS.gene69761:CDS |
| GAACTCCTTGCCATGGCAAA+AGG | 0.466099 | 3.4:+77330437 | MS.gene69761:CDS |
| TCACAAAGACACTAGAAGAA+TGG | 0.467289 | 3.4:+77334547 | MS.gene69761:CDS |
| TCAGATATACAGCATTATAA+TGG | 0.471582 | 3.4:-77330271 | None:intergenic |
| TTCCGCTTACATCTCCAGAT+TGG | 0.472380 | 3.4:+77330003 | MS.gene69761:CDS |
| CCGCGTAATGTTGAGGAAGA+TGG | 0.476953 | 3.4:-77334819 | None:intergenic |
| TCCATCATTATTCTGACTCA+TGG | 0.477097 | 3.4:-77331700 | None:intergenic |
| AAAAGAAACCTAAATTATCT+GGG | 0.477686 | 3.4:+77333017 | MS.gene69761:CDS |
| CAATCATCCATATCGAGTTA+TGG | 0.484056 | 3.4:+77328157 | MS.gene69761:CDS |
| CCCAAATCCATGCATGTCAA+TGG | 0.493456 | 3.4:-77330192 | None:intergenic |
| GGCGGTGCATCCTTGGTACT+AGG | 0.496097 | 3.4:-77334510 | None:intergenic |
| TTTACTTTATTCCATCAGAA+AGG | 0.497295 | 3.4:-77331414 | None:intergenic |
| AAAGAACCTCACCTTTCTGA+TGG | 0.498619 | 3.4:+77331403 | MS.gene69761:CDS |
| TTTGTGATAGCTTGTATGGC+TGG | 0.504362 | 3.4:-77334531 | None:intergenic |
| CCACTAATCAAAACATGTCT+AGG | 0.504970 | 3.4:+77331534 | MS.gene69761:CDS |
| CGCGTAATGTTGAGGAAGAT+GGG | 0.509064 | 3.4:-77334818 | None:intergenic |
| ACATCTCCAGATTGGAGTGC+AGG | 0.509957 | 3.4:+77330011 | MS.gene69761:CDS |
| TAGGAGAATTTCAAGCAGGT+TGG | 0.512854 | 3.4:+77332984 | MS.gene69761:intron |
| TCATATAGCCTTACAGGTAG+AGG | 0.518541 | 3.4:-77330308 | None:intergenic |
| TCAAATATGTGAACAGTACA+AGG | 0.524557 | 3.4:+77333639 | MS.gene69761:CDS |
| CATGCAGTTAGAAATTTCAA+AGG | 0.524634 | 3.4:+77334844 | MS.gene69761:CDS |
| TCCATGAGTCAGAATAATGA+TGG | 0.527396 | 3.4:+77331699 | MS.gene69761:CDS |
| CTATAGATTGTACCTGAGGT+TGG | 0.533420 | 3.4:-77331557 | None:intergenic |
| CTTGGAGAGAACTTGAACAG+TGG | 0.542544 | 3.4:-77334896 | None:intergenic |
| ATGCAGTTAGAAATTTCAAA+GGG | 0.542710 | 3.4:+77334845 | MS.gene69761:CDS |
| GAATAATGATGGAGTTAAGG+TGG | 0.546311 | 3.4:+77331710 | MS.gene69761:CDS |
| CAAGGTAACTATAAAGAAAG+TGG | 0.546753 | 3.4:+77334453 | MS.gene69761:CDS |
| AAAGGACATCAACTCAAGAA+AGG | 0.549772 | 3.4:+77330455 | MS.gene69761:CDS |
| AATGATGGAGTTAAGGTGGA+AGG | 0.553652 | 3.4:+77331714 | MS.gene69761:CDS |
| CAGAATTTATGGTTTGCTTG+TGG | 0.554923 | 3.4:+77334928 | MS.gene69761:CDS |
| TTGTTAGGAGAATTTCAAGC+AGG | 0.557337 | 3.4:+77332980 | MS.gene69761:intron |
| AACATGTCTAGGCCAACCTC+AGG | 0.568912 | 3.4:+77331545 | MS.gene69761:CDS |
| TCTTCCAAGTATAACCAAGA+AGG | 0.569053 | 3.4:+77331674 | MS.gene69761:CDS |
| ATGGTGTTGCTGAAAACGTT+GGG | 0.572743 | 3.4:+77330222 | MS.gene69761:CDS |
| CAGCTCCGAAGAATCTAAGA+AGG | 0.582538 | 3.4:+77331505 | MS.gene69761:intron |
| TGAACGTGAAACGAAACTGC+AGG | 0.594040 | 3.4:+77333168 | MS.gene69761:CDS |
| GCATGATCCGCGTAATGTTG+AGG | 0.597253 | 3.4:-77334826 | None:intergenic |
| CATGGATTTGGGAATTGGAA+TGG | 0.599035 | 3.4:+77330203 | MS.gene69761:CDS |
| CATCTATGGAAGTGTTGAGA+GGG | 0.606635 | 3.4:+77333050 | MS.gene69761:CDS |
| CCATCTATGGAAGTGTTGAG+AGG | 0.611376 | 3.4:+77333049 | MS.gene69761:CDS |
| CTTACAGGTAGAGGAAAACA+TGG | 0.611599 | 3.4:-77330299 | None:intergenic |
| CCATCTTCCTCAACATTACG+CGG | 0.613654 | 3.4:+77334819 | MS.gene69761:CDS |
| TGAGATTGAATATGACAATG+AGG | 0.615038 | 3.4:+77333096 | MS.gene69761:CDS |
| CAAAGAGGAACTCCTTGCCA+TGG | 0.618565 | 3.4:+77330430 | MS.gene69761:CDS |
| AGTGGTCAGATTGGAACAAG+CGG | 0.620881 | 3.4:+77334471 | MS.gene69761:CDS |
| AGAATCATTTCCTAGTACCA+AGG | 0.625963 | 3.4:+77334500 | MS.gene69761:CDS |
| TTTATTCCATCAGAAAGGTG+AGG | 0.628847 | 3.4:-77331409 | None:intergenic |
| AGGACAAAAGAAGCTGAACA+AGG | 0.637958 | 3.4:+77334435 | MS.gene69761:CDS |
| TCACTCAAAAGAAGAGCATG+AGG | 0.651691 | 3.4:+77333675 | MS.gene69761:CDS |
| TTCTATGGGAAAGAACAAAG+AGG | 0.651784 | 3.4:+77330415 | MS.gene69761:CDS |
| GTGATAGCTTGTATGGCTGG+CGG | 0.658411 | 3.4:-77334528 | None:intergenic |
| AATGGTGTTGCTGAAAACGT+TGG | 0.659458 | 3.4:+77330221 | MS.gene69761:CDS |
| AAAGAAACCTAAATTATCTG+GGG | 0.667221 | 3.4:+77333018 | MS.gene69761:CDS |
| AACAGTACAAGGTCTTCATG+CGG | 0.668099 | 3.4:+77333650 | MS.gene69761:CDS |
| ACGAAACTGCAGGTGCTCCG+TGG | 0.679102 | 3.4:+77333178 | MS.gene69761:CDS |
| CTCCAATCTGGAGATGTAAG+CGG | 0.681010 | 3.4:-77330005 | None:intergenic |
| AGAGGGTACAATTCTAACAG+AGG | 0.689534 | 3.4:+77333067 | MS.gene69761:CDS |
| GTAGGCATGGGAATAACTGA+TGG | 0.696306 | 3.4:+77327996 | None:intergenic |
| GTAATGTTGAGGAAGATGGG+TGG | 0.746783 | 3.4:-77334815 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 77328002 | 77334973 | 77328002 | ID=MS.gene69761 |
| chr3.4 | mRNA | 77328002 | 77334973 | 77328002 | ID=MS.gene69761.t1;Parent=MS.gene69761 |
| chr3.4 | exon | 77328002 | 77328178 | 77328002 | ID=MS.gene69761.t1.exon1;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77328002 | 77328178 | 77328002 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77329990 | 77330055 | 77329990 | ID=MS.gene69761.t1.exon2;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77329990 | 77330055 | 77329990 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77330191 | 77330316 | 77330191 | ID=MS.gene69761.t1.exon3;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77330191 | 77330316 | 77330191 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77330404 | 77330476 | 77330404 | ID=MS.gene69761.t1.exon4;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77330404 | 77330476 | 77330404 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77331374 | 77331431 | 77331374 | ID=MS.gene69761.t1.exon5;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77331374 | 77331431 | 77331374 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77331508 | 77331566 | 77331508 | ID=MS.gene69761.t1.exon6;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77331508 | 77331566 | 77331508 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77331661 | 77331735 | 77331661 | ID=MS.gene69761.t1.exon7;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77331661 | 77331735 | 77331661 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77332987 | 77333212 | 77332987 | ID=MS.gene69761.t1.exon8;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77332987 | 77333212 | 77332987 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77333546 | 77333756 | 77333546 | ID=MS.gene69761.t1.exon9;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77333546 | 77333756 | 77333546 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77334375 | 77334611 | 77334375 | ID=MS.gene69761.t1.exon10;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77334375 | 77334611 | 77334375 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| chr3.4 | exon | 77334782 | 77334973 | 77334782 | ID=MS.gene69761.t1.exon11;Parent=MS.gene69761.t1 |
| chr3.4 | CDS | 77334782 | 77334973 | 77334782 | ID=cds.MS.gene69761.t1;Parent=MS.gene69761.t1 |
| Gene Sequence |
| Protein sequence |
