AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69916.t1 MTR_1g081580 90.323 372 34 1 1 372 1 370 0.0 614
MS.gene69916.t1 MTR_7g114600 42.079 404 149 13 49 377 45 438 3.23e-85 266
MS.gene69916.t1 MTR_1g081560 37.415 294 134 10 88 374 123 373 2.04e-45 160
MS.gene69916.t1 MTR_1g081570 49.645 141 27 3 50 189 2 99 5.06e-32 117
MS.gene69916.t1 MTR_3g070120 37.037 135 75 3 97 231 153 277 9.05e-16 78.6
MS.gene69916.t1 MTR_2g010790 34.615 104 58 3 92 195 199 292 8.24e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69916.t1 AT5G28040 41.755 376 171 14 43 378 60 427 6.10e-70 226
MS.gene69916.t1 AT5G28040 41.755 376 171 14 43 378 60 427 6.10e-70 226
MS.gene69916.t1 AT3G04930 40.749 427 176 18 20 373 29 451 1.32e-59 200
MS.gene69916.t1 AT3G04930 39.589 341 145 15 20 303 29 365 1.04e-41 151
MS.gene69916.t1 AT4G00390 33.854 192 100 5 50 240 115 280 1.81e-22 98.2
MS.gene69916.t1 AT1G61730 36.646 161 92 3 35 194 97 248 5.84e-22 96.7
MS.gene69916.t1 AT4G25210 42.991 107 56 2 92 194 136 241 1.86e-20 92.4
MS.gene69916.t1 AT1G11510 44.211 95 50 1 97 191 138 229 5.29e-19 87.8
MS.gene69916.t1 AT1G11510 44.211 95 50 1 97 191 109 200 6.18e-19 87.4
MS.gene69916.t1 AT4G01260 38.365 159 71 7 61 194 51 207 3.82e-18 85.1
MS.gene69916.t1 AT4G00610 32.394 142 87 2 56 197 108 240 6.49e-18 84.3
MS.gene69916.t1 AT1G44810 38.889 108 60 2 92 199 117 218 2.63e-16 79.3
MS.gene69916.t1 AT5G14280 36.957 92 58 0 99 190 63 154 3.86e-14 72.8
MS.gene69916.t1 AT4G00238 35.135 111 64 2 98 206 141 245 6.39e-14 72.8
MS.gene69916.t1 AT4G00250 36.111 108 63 1 98 205 116 217 7.42e-14 72.4
MS.gene69916.t1 AT2G25650 34.375 96 63 0 96 191 64 159 9.86e-14 72.4
MS.gene69916.t1 AT2G01370 32.743 113 71 3 90 200 72 181 2.78e-13 69.7
MS.gene69916.t1 AT4G00270 33.333 96 64 0 96 191 63 158 9.44e-13 68.9
MS.gene69916.t1 AT1G66420 30.952 168 92 5 97 247 87 247 8.45e-12 65.9
MS.gene69916.t1 AT2G36340 29.032 93 66 0 101 193 63 155 7.35e-11 63.9

Find 120 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGTCAAATGAGAAGAATT+AGG 0.169998 1.4:-66034528 None:intergenic
CCTTACCAGAATCAAATTTC+TGG 0.175110 1.4:-66034368 None:intergenic
TAGGGTTAGGGTTGGGGTTT+GGG 0.179915 1.4:-66034509 None:intergenic
TTGAAATTTCACACAAAATC+TGG 0.187804 1.4:+66034427 MS.gene69916:CDS
TTAGGGTTAGGGTTGGGGTT+TGG 0.224438 1.4:-66034510 None:intergenic
CGCAATCTCGCAAGAGATTT+CGG 0.230365 1.4:+66034562 MS.gene69916:CDS
TCTTCCATTGGCTCCCGTTT+TGG 0.265008 1.4:-66034120 None:intergenic
TTCATCCACATTGCTGCTAT+TGG 0.293962 1.4:-66034652 None:intergenic
ACCATGAGTGGGAGTCCTTT+TGG 0.299111 1.4:+66034780 MS.gene69916:CDS
AAGAATTAGGGTTAGGGTTG+GGG 0.305318 1.4:-66034515 None:intergenic
CTTATCAACAAGCTGGTTCT+TGG 0.314691 1.4:-66034310 None:intergenic
GCAATCTCGCAAGAGATTTC+GGG 0.321881 1.4:+66034563 MS.gene69916:CDS
CAATGAATACTGTGCCATTT+GGG 0.343845 1.4:+66034838 MS.gene69916:CDS
CAGTCAAATGAGAAGAATTA+GGG 0.345673 1.4:-66034527 None:intergenic
GCTCTCCAGAAATTTGATTC+TGG 0.345969 1.4:+66034363 MS.gene69916:CDS
GAAGAATTAGGGTTAGGGTT+GGG 0.347770 1.4:-66034516 None:intergenic
TCAATGAATACTGTGCCATT+TGG 0.350621 1.4:+66034837 MS.gene69916:CDS
AAATGAGAAGAATTAGGGTT+AGG 0.361264 1.4:-66034522 None:intergenic
ATTGCTGCTATTGGTATTAC+CGG 0.365150 1.4:-66034643 None:intergenic
TCCTCCTATCATAACGATAC+TGG 0.368215 1.4:+66034243 MS.gene69916:CDS
GGAGGTGGGGACAGCGGCGG+TGG 0.386670 1.4:-66034037 None:intergenic
TGATAGTGGTGGTCGGTGTT+TGG 0.389979 1.4:-66033988 None:intergenic
GTGGCGGAAGCAGCAGATCT+TGG 0.391074 1.4:+66034902 MS.gene69916:CDS
ATATGATATTATCTATTGAT+TGG 0.394784 1.4:-66035048 None:intergenic
GCTGGAGCCGGAGCCGGAGC+TGG 0.400813 1.4:+66035008 MS.gene69916:CDS
TGAAATTTCACACAAAATCT+GGG 0.411047 1.4:+66034428 MS.gene69916:CDS
CGGCTCCCCTGAACCAAAAC+GGG 0.425461 1.4:+66034107 MS.gene69916:CDS
ACCAGCTTGTTGATAAGCTT+CGG 0.428179 1.4:+66034316 MS.gene69916:CDS
ATTTCCTCCTCTTCATCAAC+AGG 0.428859 1.4:-66034483 None:intergenic
TGTAATTGAGAAACCCTTGC+AGG 0.430967 1.4:-66034206 None:intergenic
GGAGCCGGAGCTGGAGGTAG+TGG 0.440600 1.4:+66035017 MS.gene69916:CDS
AATGAGAAGAATTAGGGTTA+GGG 0.441702 1.4:-66034521 None:intergenic
GGGGACAGCGGCGGTGGCGA+AGG 0.444218 1.4:-66034031 None:intergenic
GCTGGAGCTGGAGCCGGAGC+CGG 0.444290 1.4:+66035002 MS.gene69916:CDS
CGATCCAGTATCGTTATGAT+AGG 0.445812 1.4:-66034247 None:intergenic
CCAGAAATTTGATTCTGGTA+AGG 0.445820 1.4:+66034368 MS.gene69916:CDS
TTTGTGTGAAATTTCAAAAG+TGG 0.448452 1.4:-66034421 None:intergenic
GAAGGTTGACGGAGATTGTT+CGG 0.453008 1.4:-66034013 None:intergenic
CTGTTTGACACCGGTGTTGA+AGG 0.458464 1.4:+66034743 MS.gene69916:CDS
CTGATGCGGAGGCGTGTGGT+AGG 0.462324 1.4:-66034071 None:intergenic
AAGAGAAGACCAGCGATTCT+CGG 0.466132 1.4:+66034139 MS.gene69916:CDS
AACGGTAATTCAACGCCAAT+TGG 0.467910 1.4:+66034456 MS.gene69916:CDS
TCGCTGGTCTTCTCTTCCAT+TGG 0.470929 1.4:-66034132 None:intergenic
TCATCAACAGGTACACCAAT+TGG 0.471428 1.4:-66034471 None:intergenic
GCGGCGGTGGCGAAGGTTGA+CGG 0.475689 1.4:-66034024 None:intergenic
TGGTTCAGGGGAGCCGTCGA+TGG 0.476050 1.4:-66034100 None:intergenic
CGATGTCGGCGTAATCATCA+TGG 0.477676 1.4:-66033915 None:intergenic
GAGATTGTTCGGTTTGATAG+TGG 0.479733 1.4:-66034002 None:intergenic
ATTTCCACTACCTCCAGCTC+CGG 0.484287 1.4:-66035021 None:intergenic
TAGGGCTAAGATGACGGAGG+TGG 0.485162 1.4:-66034052 None:intergenic
ACTACCTCCAGCTCCGGCTC+CGG 0.492756 1.4:-66035015 None:intergenic
CGGTGTCAAACAGCTCTTCA+CGG 0.494859 1.4:-66034733 None:intergenic
GAAGCAGCAGATCTTGGAGC+TGG 0.497446 1.4:+66034908 MS.gene69916:CDS
CACAAAATCTGGGGCAGCAA+CGG 0.505174 1.4:+66034438 MS.gene69916:CDS
ACGGCTCCCCTGAACCAAAA+CGG 0.512535 1.4:+66034106 MS.gene69916:CDS
TTGAGATCAAATGCTGGAGC+TGG 0.514156 1.4:+66034990 MS.gene69916:CDS
GAAGCGGTTGGAGCTGGTGC+AGG 0.515736 1.4:+66034941 MS.gene69916:CDS
ACGGTCTCTTCGATTAATCC+AGG 0.519165 1.4:-66034714 None:intergenic
CTCATGGCATTCTCTCGAAA+CGG 0.520566 1.4:+66033877 None:intergenic
TCAAATGCTGGAGCTGGAGC+CGG 0.521072 1.4:+66034996 MS.gene69916:CDS
AGGGCTAAGATGACGGAGGT+GGG 0.524316 1.4:-66034051 None:intergenic
GTATTCGAAGCGGTTGGAGC+TGG 0.525755 1.4:+66034935 MS.gene69916:CDS
AGTGGTGGTCGGTGTTTGGT+TGG 0.541859 1.4:-66033984 None:intergenic
GGAGCCGGAGCCGGAGCTGG+AGG 0.542893 1.4:+66035011 MS.gene69916:CDS
GAGATTTCGGGCAGCTGAGG+AGG 0.545575 1.4:+66034575 MS.gene69916:CDS
ATCGTTCTATGATCACATGA+AGG 0.546638 1.4:+66034266 MS.gene69916:CDS
TTCGGTTTGATAGTGGTGGT+CGG 0.548380 1.4:-66033995 None:intergenic
TGAGATCAAGGTTGCGATCG+AGG 0.551018 1.4:+66034965 MS.gene69916:CDS
TGAACCAAAACGGGAGCCAA+TGG 0.551623 1.4:+66034116 MS.gene69916:CDS
CAAGAGATTTCGGGCAGCTG+AGG 0.556173 1.4:+66034572 MS.gene69916:CDS
CATGAAGGGGAAGATTCAGC+TGG 0.556910 1.4:+66034281 MS.gene69916:CDS
TCCGAAGCTTATCAACAAGC+TGG 0.557121 1.4:-66034317 None:intergenic
CGACGACGATTTAGAAGAAG+AGG 0.558405 1.4:+66033948 MS.gene69916:CDS
GATGACGGAGGTGGGGACAG+CGG 0.558674 1.4:-66034043 None:intergenic
GACGAAATCACGATCCTGCA+AGG 0.561286 1.4:+66034192 MS.gene69916:CDS
TCCAGTATCGTTATGATAGG+AGG 0.562775 1.4:-66034244 None:intergenic
AGAAGAATTAGGGTTAGGGT+TGG 0.562837 1.4:-66034517 None:intergenic
CAAATTTCTGGAGAGCAGTA+CGG 0.564956 1.4:-66034356 None:intergenic
GTCGTCTTCTCCCTCGATGT+CGG 0.573199 1.4:-66033929 None:intergenic
AATCATGCACATAATGTACC+TGG 0.574034 1.4:+66034696 MS.gene69916:CDS
GATGATTACGCCGACATCGA+GGG 0.574634 1.4:+66033919 MS.gene69916:CDS
ATTGTTCGGTTTGATAGTGG+TGG 0.575227 1.4:-66033999 None:intergenic
TCGGCGTAATCATCATGGGT+GGG 0.575332 1.4:-66033910 None:intergenic
TCGTTCTATGATCACATGAA+GGG 0.576436 1.4:+66034267 MS.gene69916:CDS
TTCGATAACTCCTTCAACAC+CGG 0.578505 1.4:-66034753 None:intergenic
GAGGAGTTGAGATCAAATGC+TGG 0.581161 1.4:+66034984 MS.gene69916:CDS
GTGTGGTAGGGCTAAGATGA+CGG 0.582706 1.4:-66034058 None:intergenic
GGAGGTGTATTCGAAGCGGT+TGG 0.582984 1.4:+66034929 MS.gene69916:CDS
GAAAATCGTGATAGAAGTAA+CGG 0.584019 1.4:+66034672 MS.gene69916:CDS
TGATGCGGAGGCGTGTGGTA+GGG 0.584219 1.4:-66034070 None:intergenic
AAAAGGCACTGGATGAAAAG+TGG 0.591957 1.4:+66034883 MS.gene69916:CDS
GTCGATGGTTGTTGCTGATG+CGG 0.592509 1.4:-66034085 None:intergenic
CGGCGTAATCATCATGGGTG+GGG 0.593339 1.4:-66033909 None:intergenic
GCTGGTGCAGGATGAGATCA+AGG 0.595852 1.4:+66034953 MS.gene69916:CDS
GATAATCATCAGAATAACTC+CGG 0.596997 1.4:+66034624 MS.gene69916:CDS
ACCAAAAGGACTCCCACTCA+TGG 0.598783 1.4:-66034781 None:intergenic
TTATCGAATGCTACCATGAG+TGG 0.599446 1.4:+66034768 MS.gene69916:CDS
GTCGGCGTAATCATCATGGG+TGG 0.603570 1.4:-66033911 None:intergenic
TTTCGTCTTCTTCAGTCCAG+AGG 0.609633 1.4:-66034176 None:intergenic
GGGCTAAGATGACGGAGGTG+GGG 0.612836 1.4:-66034050 None:intergenic
GAAATTTCACACAAAATCTG+GGG 0.613296 1.4:+66034429 MS.gene69916:CDS
GATGTCGGCGTAATCATCAT+GGG 0.614670 1.4:-66033914 None:intergenic
CGTTCTATGATCACATGAAG+GGG 0.621138 1.4:+66034268 MS.gene69916:CDS
GACGGAGGTGGGGACAGCGG+CGG 0.622679 1.4:-66034040 None:intergenic
AGCTGGAGGTGTATTCGAAG+CGG 0.623559 1.4:+66034925 MS.gene69916:CDS
TGGTGTACCTGTTGATGAAG+AGG 0.630690 1.4:+66034476 MS.gene69916:CDS
GTTGCTGATGCGGAGGCGTG+TGG 0.634510 1.4:-66034075 None:intergenic
CGTGAAGAGCTGTTTGACAC+CGG 0.636688 1.4:+66034734 MS.gene69916:CDS
ACGAAATCACGATCCTGCAA+GGG 0.639447 1.4:+66034193 MS.gene69916:CDS
TGATGATTACGCCGACATCG+AGG 0.642948 1.4:+66033918 MS.gene69916:CDS
AGCTTGTTGATAAGCTTCGG+AGG 0.643387 1.4:+66034319 MS.gene69916:CDS
TATCGAATGCTACCATGAGT+GGG 0.643483 1.4:+66034769 MS.gene69916:CDS
GGCATTCTCTCGAAACGGCG+TGG 0.645669 1.4:+66033882 MS.gene69916:CDS
GCAGCAGATCTTGGAGCTGG+AGG 0.646814 1.4:+66034911 MS.gene69916:CDS
GCATCAGCAACAACCATCGA+CGG 0.685479 1.4:+66034087 MS.gene69916:CDS
AGGCACTGGATGAAAAGTGG+CGG 0.689339 1.4:+66034886 MS.gene69916:CDS
TAATACCAATAGCAGCAATG+TGG 0.690177 1.4:+66034647 MS.gene69916:CDS
GATGGTTGTTGCTGATGCGG+AGG 0.694249 1.4:-66034082 None:intergenic
TGTACCTGTTGATGAAGAGG+AGG 0.712098 1.4:+66034479 MS.gene69916:CDS
TGGTAGGGCTAAGATGACGG+AGG 0.739720 1.4:-66034055 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGAAATTTCACACAAAATCT+GGG + chr1.4:66034428-66034447 MS.gene69916:CDS 25.0%
! TTGAAATTTCACACAAAATC+TGG + chr1.4:66034427-66034446 MS.gene69916:CDS 25.0%
! TTTGTGTGAAATTTCAAAAG+TGG - chr1.4:66034424-66034443 None:intergenic 25.0%
AAATGAGAAGAATTAGGGTT+AGG - chr1.4:66034525-66034544 None:intergenic 30.0%
AATGAGAAGAATTAGGGTTA+GGG - chr1.4:66034524-66034543 None:intergenic 30.0%
CAGTCAAATGAGAAGAATTA+GGG - chr1.4:66034530-66034549 None:intergenic 30.0%
GAAAATCGTGATAGAAGTAA+CGG + chr1.4:66034672-66034691 MS.gene69916:CDS 30.0%
GAAATTTCACACAAAATCTG+GGG + chr1.4:66034429-66034448 MS.gene69916:CDS 30.0%
GATAATCATCAGAATAACTC+CGG + chr1.4:66034624-66034643 MS.gene69916:CDS 30.0%
TCAGTCAAATGAGAAGAATT+AGG - chr1.4:66034531-66034550 None:intergenic 30.0%
!! ATTTGGGTTTTTGAATTTGG+GGG + chr1.4:66034854-66034873 MS.gene69916:CDS 30.0%
!! CATTTGGGTTTTTGAATTTG+GGG + chr1.4:66034853-66034872 MS.gene69916:CDS 30.0%
!! CCATTTGGGTTTTTGAATTT+GGG + chr1.4:66034852-66034871 MS.gene69916:CDS 30.0%
AATCATGCACATAATGTACC+TGG + chr1.4:66034696-66034715 MS.gene69916:CDS 35.0%
ATCGTTCTATGATCACATGA+AGG + chr1.4:66034266-66034285 MS.gene69916:CDS 35.0%
ATTGCTGCTATTGGTATTAC+CGG - chr1.4:66034646-66034665 None:intergenic 35.0%
CCAGAAATTTGATTCTGGTA+AGG + chr1.4:66034368-66034387 MS.gene69916:CDS 35.0%
CCCAAATTCAAAAACCCAAA+TGG - chr1.4:66034855-66034874 None:intergenic 35.0%
CCTTACCAGAATCAAATTTC+TGG - chr1.4:66034371-66034390 None:intergenic 35.0%
TAATACCAATAGCAGCAATG+TGG + chr1.4:66034647-66034666 MS.gene69916:CDS 35.0%
TCGTTCTATGATCACATGAA+GGG + chr1.4:66034267-66034286 MS.gene69916:CDS 35.0%
! TCAATGAATACTGTGCCATT+TGG + chr1.4:66034837-66034856 MS.gene69916:CDS 35.0%
!! CAATGAATACTGTGCCATTT+GGG + chr1.4:66034838-66034857 MS.gene69916:CDS 35.0%
!! GCCATTTGGGTTTTTGAATT+TGG + chr1.4:66034851-66034870 MS.gene69916:CDS 35.0%
!! TTTGGGTTTTTGAATTTGGG+GGG + chr1.4:66034855-66034874 MS.gene69916:CDS 35.0%
AACGGTAATTCAACGCCAAT+TGG + chr1.4:66034456-66034475 MS.gene69916:CDS 40.0%
AAGAATTAGGGTTAGGGTTG+GGG - chr1.4:66034518-66034537 None:intergenic 40.0%
AGAAGAATTAGGGTTAGGGT+TGG - chr1.4:66034520-66034539 None:intergenic 40.0%
ATTTCCTCCTCTTCATCAAC+AGG - chr1.4:66034486-66034505 None:intergenic 40.0%
CGATCCAGTATCGTTATGAT+AGG - chr1.4:66034250-66034269 None:intergenic 40.0%
CGTTCTATGATCACATGAAG+GGG + chr1.4:66034268-66034287 MS.gene69916:CDS 40.0%
GAAGAATTAGGGTTAGGGTT+GGG - chr1.4:66034519-66034538 None:intergenic 40.0%
GCTCTCCAGAAATTTGATTC+TGG + chr1.4:66034363-66034382 MS.gene69916:CDS 40.0%
TATCGAATGCTACCATGAGT+GGG + chr1.4:66034769-66034788 MS.gene69916:CDS 40.0%
TCATCAACAGGTACACCAAT+TGG - chr1.4:66034474-66034493 None:intergenic 40.0%
TCCAGTATCGTTATGATAGG+AGG - chr1.4:66034247-66034266 None:intergenic 40.0%
TCCTCCTATCATAACGATAC+TGG + chr1.4:66034243-66034262 MS.gene69916:CDS 40.0%
TGTAATTGAGAAACCCTTGC+AGG - chr1.4:66034209-66034228 None:intergenic 40.0%
TTATCGAATGCTACCATGAG+TGG + chr1.4:66034768-66034787 MS.gene69916:CDS 40.0%
TTCATCCACATTGCTGCTAT+TGG - chr1.4:66034655-66034674 None:intergenic 40.0%
TTCGATAACTCCTTCAACAC+CGG - chr1.4:66034756-66034775 None:intergenic 40.0%
! CAAATTTCTGGAGAGCAGTA+CGG - chr1.4:66034359-66034378 None:intergenic 40.0%
! CTTATCAACAAGCTGGTTCT+TGG - chr1.4:66034313-66034332 None:intergenic 40.0%
! GAGATTGTTCGGTTTGATAG+TGG - chr1.4:66034005-66034024 None:intergenic 40.0%
!! AAAAGGCACTGGATGAAAAG+TGG + chr1.4:66034883-66034902 MS.gene69916:CDS 40.0%
!! ACCAGCTTGTTGATAAGCTT+CGG + chr1.4:66034316-66034335 MS.gene69916:CDS 40.0%
!! ATTGTTCGGTTTGATAGTGG+TGG - chr1.4:66034002-66034021 None:intergenic 40.0%
AAAAAGCTTCCGAGAATCGC+TGG - chr1.4:66034151-66034170 None:intergenic 45.0%
AAGAGAAGACCAGCGATTCT+CGG + chr1.4:66034139-66034158 MS.gene69916:CDS 45.0%
ACGAAATCACGATCCTGCAA+GGG + chr1.4:66034193-66034212 MS.gene69916:CDS 45.0%
ACGGTCTCTTCGATTAATCC+AGG - chr1.4:66034717-66034736 None:intergenic 45.0%
CGACGACGATTTAGAAGAAG+AGG + chr1.4:66033948-66033967 MS.gene69916:CDS 45.0%
CGCAATCTCGCAAGAGATTT+CGG + chr1.4:66034562-66034581 MS.gene69916:CDS 45.0%
GAAGGTTGACGGAGATTGTT+CGG - chr1.4:66034016-66034035 None:intergenic 45.0%
GAGGAGTTGAGATCAAATGC+TGG + chr1.4:66034984-66035003 MS.gene69916:CDS 45.0%
GATGTCGGCGTAATCATCAT+GGG - chr1.4:66033917-66033936 None:intergenic 45.0%
GCAATCTCGCAAGAGATTTC+GGG + chr1.4:66034563-66034582 MS.gene69916:CDS 45.0%
TCCGAAGCTTATCAACAAGC+TGG - chr1.4:66034320-66034339 None:intergenic 45.0%
TTGAGATCAAATGCTGGAGC+TGG + chr1.4:66034990-66035009 MS.gene69916:CDS 45.0%
TTTCGTCTTCTTCAGTCCAG+AGG - chr1.4:66034179-66034198 None:intergenic 45.0%
! TGGTGTACCTGTTGATGAAG+AGG + chr1.4:66034476-66034495 MS.gene69916:CDS 45.0%
! TGTACCTGTTGATGAAGAGG+AGG + chr1.4:66034479-66034498 MS.gene69916:CDS 45.0%
!! AGCTTGTTGATAAGCTTCGG+AGG + chr1.4:66034319-66034338 MS.gene69916:CDS 45.0%
!! TTCGGTTTGATAGTGGTGGT+CGG - chr1.4:66033998-66034017 None:intergenic 45.0%
ACCAAAAGGACTCCCACTCA+TGG - chr1.4:66034784-66034803 None:intergenic 50.0%
ATTTCCACTACCTCCAGCTC+CGG - chr1.4:66035024-66035043 None:intergenic 50.0%
CATGAAGGGGAAGATTCAGC+TGG + chr1.4:66034281-66034300 MS.gene69916:CDS 50.0%
CGATGTCGGCGTAATCATCA+TGG - chr1.4:66033918-66033937 None:intergenic 50.0%
CGTGAAGAGCTGTTTGACAC+CGG + chr1.4:66034734-66034753 MS.gene69916:CDS 50.0%
GACGAAATCACGATCCTGCA+AGG + chr1.4:66034192-66034211 MS.gene69916:CDS 50.0%
GATGATTACGCCGACATCGA+GGG + chr1.4:66033919-66033938 MS.gene69916:CDS 50.0%
GCATCAGCAACAACCATCGA+CGG + chr1.4:66034087-66034106 MS.gene69916:CDS 50.0%
GTCGATGGTTGTTGCTGATG+CGG - chr1.4:66034088-66034107 None:intergenic 50.0%
GTGTGGTAGGGCTAAGATGA+CGG - chr1.4:66034061-66034080 None:intergenic 50.0%
TAGGGTTAGGGTTGGGGTTT+GGG - chr1.4:66034512-66034531 None:intergenic 50.0%
TCGGCGTAATCATCATGGGT+GGG - chr1.4:66033913-66033932 None:intergenic 50.0%
TGAACCAAAACGGGAGCCAA+TGG + chr1.4:66034116-66034135 MS.gene69916:CDS 50.0%
TGAGATCAAGGTTGCGATCG+AGG + chr1.4:66034965-66034984 MS.gene69916:CDS 50.0%
TGATGATTACGCCGACATCG+AGG + chr1.4:66033918-66033937 MS.gene69916:CDS 50.0%
TTAGGGTTAGGGTTGGGGTT+TGG - chr1.4:66034513-66034532 None:intergenic 50.0%
! ACCATGAGTGGGAGTCCTTT+TGG + chr1.4:66034780-66034799 MS.gene69916:CDS 50.0%
! AGCTGGAGGTGTATTCGAAG+CGG + chr1.4:66034925-66034944 MS.gene69916:CDS 50.0%
! AGTGGGAGTCCTTTTGGTCT+TGG + chr1.4:66034786-66034805 MS.gene69916:CDS 50.0%
! CACAAAATCTGGGGCAGCAA+CGG + chr1.4:66034438-66034457 MS.gene69916:CDS 50.0%
! CGGTGTCAAACAGCTCTTCA+CGG - chr1.4:66034736-66034755 None:intergenic 50.0%
! CTGTTTGACACCGGTGTTGA+AGG + chr1.4:66034743-66034762 MS.gene69916:CDS 50.0%
! GGGTTTTTGAATTTGGGGGG+CGG + chr1.4:66034858-66034877 MS.gene69916:CDS 50.0%
!! AGGCACTGGATGAAAAGTGG+CGG + chr1.4:66034886-66034905 MS.gene69916:CDS 50.0%
!! GTGGGAGTCCTTTTGGTCTT+GGG + chr1.4:66034787-66034806 MS.gene69916:CDS 50.0%
!! TCGCTGGTCTTCTCTTCCAT+TGG - chr1.4:66034135-66034154 None:intergenic 50.0%
!! TGATAGTGGTGGTCGGTGTT+TGG - chr1.4:66033991-66034010 None:intergenic 50.0%
!!! ATTGGCTCCCGTTTTGGTTC+AGG - chr1.4:66034117-66034136 None:intergenic 50.0%
!!! CTTTTGGTCTTGGGGGAAGA+GGG + chr1.4:66034796-66034815 MS.gene69916:CDS 50.0%
!!! TCTTCCATTGGCTCCCGTTT+TGG - chr1.4:66034123-66034142 None:intergenic 50.0%
!!! TGGGAGTCCTTTTGGTCTTG+GGG + chr1.4:66034788-66034807 MS.gene69916:CDS 50.0%
!!! TTGGCTCCCGTTTTGGTTCA+GGG - chr1.4:66034116-66034135 None:intergenic 50.0%
ACGGCTCCCCTGAACCAAAA+CGG + chr1.4:66034106-66034125 MS.gene69916:CDS 55.0%
CAAGAGATTTCGGGCAGCTG+AGG + chr1.4:66034572-66034591 MS.gene69916:CDS 55.0%
CCTCTTCCCCCAAGACCAAA+AGG - chr1.4:66034798-66034817 None:intergenic 55.0%
CGGCGTAATCATCATGGGTG+GGG - chr1.4:66033912-66033931 None:intergenic 55.0%
GAAGCAGCAGATCTTGGAGC+TGG + chr1.4:66034908-66034927 MS.gene69916:CDS 55.0%
GAATTTGGGGGGCGGAGAAA+AGG + chr1.4:66034866-66034885 MS.gene69916:CDS 55.0%
GATGGTTGTTGCTGATGCGG+AGG - chr1.4:66034085-66034104 None:intergenic 55.0%
GCTGGTGCAGGATGAGATCA+AGG + chr1.4:66034953-66034972 MS.gene69916:CDS 55.0%
GTATTCGAAGCGGTTGGAGC+TGG + chr1.4:66034935-66034954 MS.gene69916:CDS 55.0%
GTCGGCGTAATCATCATGGG+TGG - chr1.4:66033914-66033933 None:intergenic 55.0%
GTCGTCTTCTCCCTCGATGT+CGG - chr1.4:66033932-66033951 None:intergenic 55.0%
TCAAATGCTGGAGCTGGAGC+CGG + chr1.4:66034996-66035015 MS.gene69916:CDS 55.0%
! AGTGGTGGTCGGTGTTTGGT+TGG - chr1.4:66033987-66034006 None:intergenic 55.0%
! GGAGGTGTATTCGAAGCGGT+TGG + chr1.4:66034929-66034948 MS.gene69916:CDS 55.0%
! TGGTAGGGCTAAGATGACGG+AGG - chr1.4:66034058-66034077 None:intergenic 55.0%
!! AGGGCTAAGATGACGGAGGT+GGG - chr1.4:66034054-66034073 None:intergenic 55.0%
!! TAGGGCTAAGATGACGGAGG+TGG - chr1.4:66034055-66034074 None:intergenic 55.0%
!!! CCTTTTGGTCTTGGGGGAAG+AGG + chr1.4:66034795-66034814 MS.gene69916:CDS 55.0%
!!! GGAAGCTTTTTCAGCGCCTC+TGG + chr1.4:66034160-66034179 MS.gene69916:CDS 55.0%
!!! GGGAGTCCTTTTGGTCTTGG+GGG + chr1.4:66034789-66034808 MS.gene69916:CDS 55.0%
!!! TGGCTCCCGTTTTGGTTCAG+GGG - chr1.4:66034115-66034134 None:intergenic 55.0%
CGGCTCCCCTGAACCAAAAC+GGG + chr1.4:66034107-66034126 MS.gene69916:CDS 60.0%
GAGATTTCGGGCAGCTGAGG+AGG + chr1.4:66034575-66034594 MS.gene69916:CDS 60.0%
GCAGCAGATCTTGGAGCTGG+AGG + chr1.4:66034911-66034930 MS.gene69916:CDS 60.0%
GGCATTCTCTCGAAACGGCG+TGG + chr1.4:66033882-66033901 MS.gene69916:CDS 60.0%
GGTCTTGGGGGAAGAGGGTT+TGG + chr1.4:66034801-66034820 MS.gene69916:CDS 60.0%
! GTGGCGGAAGCAGCAGATCT+TGG + chr1.4:66034902-66034921 MS.gene69916:CDS 60.0%
! TGATGCGGAGGCGTGTGGTA+GGG - chr1.4:66034073-66034092 None:intergenic 60.0%
!! GGGCTAAGATGACGGAGGTG+GGG - chr1.4:66034053-66034072 None:intergenic 60.0%
ACTACCTCCAGCTCCGGCTC+CGG - chr1.4:66035018-66035037 None:intergenic 65.0%
GAAGCGGTTGGAGCTGGTGC+AGG + chr1.4:66034941-66034960 MS.gene69916:CDS 65.0%
TGGTTCAGGGGAGCCGTCGA+TGG - chr1.4:66034103-66034122 None:intergenic 65.0%
! CTGATGCGGAGGCGTGTGGT+AGG - chr1.4:66034074-66034093 None:intergenic 65.0%
! GGGGGAAGAGGGTTTGGAGC+TGG + chr1.4:66034807-66034826 MS.gene69916:CDS 65.0%
! GTTGCTGATGCGGAGGCGTG+TGG - chr1.4:66034078-66034097 None:intergenic 65.0%
!! GATGACGGAGGTGGGGACAG+CGG - chr1.4:66034046-66034065 None:intergenic 65.0%
GCGGCGGTGGCGAAGGTTGA+CGG - chr1.4:66034027-66034046 None:intergenic 70.0%
GGAGCCGGAGCTGGAGGTAG+TGG + chr1.4:66035017-66035036 MS.gene69916:CDS 70.0%
! GGGGGGCGGAGAAAAGGCAC+TGG + chr1.4:66034872-66034891 MS.gene69916:CDS 70.0%
GACGGAGGTGGGGACAGCGG+CGG - chr1.4:66034043-66034062 None:intergenic 75.0%
GCTGGAGCTGGAGCCGGAGC+CGG + chr1.4:66035002-66035021 MS.gene69916:CDS 75.0%
! GCTGGAGCCGGAGCCGGAGC+TGG + chr1.4:66035008-66035027 MS.gene69916:CDS 80.0%
! GGAGCCGGAGCCGGAGCTGG+AGG + chr1.4:66035011-66035030 MS.gene69916:CDS 80.0%
! GGAGGTGGGGACAGCGGCGG+TGG - chr1.4:66034040-66034059 None:intergenic 80.0%
! GGGGACAGCGGCGGTGGCGA+AGG - chr1.4:66034034-66034053 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 66033880 66035058 66033880 ID=MS.gene69916
chr1.4 mRNA 66033880 66035058 66033880 ID=MS.gene69916.t1;Parent=MS.gene69916
chr1.4 exon 66033880 66035058 66033880 ID=MS.gene69916.t1.exon1;Parent=MS.gene69916.t1
chr1.4 CDS 66033880 66035058 66033880 ID=cds.MS.gene69916.t1;Parent=MS.gene69916.t1
Gene Sequence

>MS.gene69916

ATGGCATTCTCTCGAAACGGCGTGGTTATCCCCACCCATGATGATTACGCCGACATCGAGGGAGAAGACGACGACGATTTAGAAGAAGAGGACAACTACTTTGTCCAACCAAACACCGACCACCACTATCAAACCGAACAATCTCCGTCAACCTTCGCCACCGCCGCTGTCCCCACCTCCGTCATCTTAGCCCTACCACACGCCTCCGCATCAGCAACAACCATCGACGGCTCCCCTGAACCAAAACGGGAGCCAATGGAAGAGAAGACCAGCGATTCTCGGAAGCTTTTTCAGCGCCTCTGGACTGAAGAAGACGAAATCACGATCCTGCAAGGGTTTCTCAATTACAACGCTAATCGTGAATCCTCCTATCATAACGATACTGGATCGTTCTATGATCACATGAAGGGGAAGATTCAGCTGGATTTCACCAAGAACCAGCTTGTTGATAAGCTTCGGAGGCTGAAGAAGAAGTACCGTACTGCTCTCCAGAAATTTGATTCTGGTAAGGATTTTACTTTCAAAAGTGCTCATGATCAAGCCACTTTTGAAATTTCACACAAAATCTGGGGCAGCAACGGTAATTCAACGCCAATTGGTGTACCTGTTGATGAAGAGGAGGAAATGAGCCCAAACCCCAACCCTAACCCTAATTCTTCTCATTTGACTGAGAAGAAAGCTGCGCAATCTCGCAAGAGATTTCGGGCAGCTGAGGAGGAAAAGCGTGAGTTGACTGATTCTAGAGATAATCATCAGAATAACTCCGGTAATACCAATAGCAGCAATGTGGATGAAAATCGTGATAGAAGTAACGGTAATCATGCACATAATGTACCTGGATTAATCGAAGAGACCGTGAAGAGCTGTTTGACACCGGTGTTGAAGGAGTTATCGAATGCTACCATGAGTGGGAGTCCTTTTGGTCTTGGGGGAAGAGGGTTTGGAGCTGGAGCTTTTTCAATGAATACTGTGCCATTTGGGTTTTTGAATTTGGGGGGCGGAGAAAAGGCACTGGATGAAAAGTGGCGGAAGCAGCAGATCTTGGAGCTGGAGGTGTATTCGAAGCGGTTGGAGCTGGTGCAGGATGAGATCAAGGTTGCGATCGAGGAGTTGAGATCAAATGCTGGAGCTGGAGCCGGAGCCGGAGCTGGAGGTAGTGGAAATAATACCAATCAATAG
Protein sequence

>MS.gene69916.t1

MAFSRNGVVIPTHDDYADIEGEDDDDLEEEDNYFVQPNTDHHYQTEQSPSTFATAAVPTSVILALPHASASATTIDGSPEPKREPMEEKTSDSRKLFQRLWTEEDEITILQGFLNYNANRESSYHNDTGSFYDHMKGKIQLDFTKNQLVDKLRRLKKKYRTALQKFDSGKDFTFKSAHDQATFEISHKIWGSNGNSTPIGVPVDEEEEMSPNPNPNPNSSHLTEKKAAQSRKRFRAAEEEKRELTDSRDNHQNNSGNTNSSNVDENRDRSNGNHAHNVPGLIEETVKSCLTPVLKELSNATMSGSPFGLGGRGFGAGAFSMNTVPFGFLNLGGGEKALDEKWRKQQILELEVYSKRLELVQDEIKVAIEELRSNAGAGAGAGAGGSGNNTNQ