AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70003

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70003.t1 MTR_8g072590 82.504 663 80 7 1 632 1 658 0.0 1080
MS.gene70003.t1 MTR_8g073190 51.639 732 208 22 17 622 21 732 0.0 651
MS.gene70003.t1 MTR_5g024510 37.244 733 304 24 38 622 65 789 3.76e-126 397
MS.gene70003.t1 MTR_5g024510 36.286 700 290 24 38 589 65 756 1.78e-113 360
MS.gene70003.t1 MTR_5g024510 36.228 668 274 22 102 622 5 667 5.53e-106 340
MS.gene70003.t1 MTR_5g026960 27.304 293 155 13 354 603 8 285 6.62e-18 87.4
MS.gene70003.t1 MTR_8g012450 26.816 358 198 14 348 658 2 342 2.00e-16 82.4
MS.gene70003.t1 MTR_8g012450 27.759 299 155 12 348 601 2 284 6.28e-16 81.3
MS.gene70003.t1 MTR_4g086660 29.617 287 151 16 350 603 133 401 1.27e-15 80.9
MS.gene70003.t1 MTR_3g092230 28.621 290 170 14 343 608 131 407 4.83e-14 75.9
MS.gene70003.t1 MTR_2g020380 27.675 271 166 8 356 610 10 266 5.41e-14 76.6
MS.gene70003.t1 MTR_8g024590 28.214 280 169 12 343 610 20 279 5.67e-14 75.1
MS.gene70003.t1 MTR_8g024600 27.857 280 170 12 343 610 20 279 1.50e-13 73.9
MS.gene70003.t1 MTR_7g089480 26.361 349 205 17 285 603 79 405 1.94e-13 73.9
MS.gene70003.t1 MTR_7g118020 27.070 314 178 17 325 606 117 411 2.85e-13 73.6
MS.gene70003.t1 MTR_8g024100 27.431 288 173 12 344 610 10 282 3.25e-13 73.6
MS.gene70003.t1 MTR_1g105290 26.882 279 166 10 343 601 58 318 3.33e-13 73.6
MS.gene70003.t1 MTR_1g105290 26.882 279 166 10 343 601 58 318 3.98e-13 72.8
MS.gene70003.t1 MTR_5g017830 25.872 344 192 16 294 603 84 398 4.08e-13 73.2
MS.gene70003.t1 MTR_1g105290 26.882 279 166 10 343 601 58 318 4.42e-13 73.2
MS.gene70003.t1 MTR_8g079790 27.469 324 173 16 334 613 96 401 1.92e-12 70.9
MS.gene70003.t1 MTR_1g105595 25.414 362 206 16 204 533 207 536 2.00e-12 70.9
MS.gene70003.t1 MTR_5g022030 26.117 291 178 14 343 615 102 373 2.35e-12 70.5
MS.gene70003.t1 MTR_8g093730 26.383 235 154 6 386 615 349 569 2.90e-12 70.5
MS.gene70003.t1 MTR_2g016340 25.172 290 168 12 347 608 5 273 4.03e-12 69.3
MS.gene70003.t1 MTR_5g022030 26.117 291 178 14 343 615 102 373 4.15e-12 69.7
MS.gene70003.t1 MTR_4g114670 25.461 271 176 8 343 604 14 267 5.11e-12 68.6
MS.gene70003.t1 MTR_7g113490 28.395 243 143 8 371 600 106 330 6.32e-12 69.3
MS.gene70003.t1 MTR_4g114670 25.461 271 176 8 343 604 14 267 6.56e-12 68.6
MS.gene70003.t1 MTR_5g092810 25.352 284 169 12 344 602 86 351 8.16e-12 68.9
MS.gene70003.t1 MTR_8g095440 24.468 282 172 10 344 601 24 288 1.37e-11 67.4
MS.gene70003.t1 MTR_3g096230 26.582 237 154 7 386 616 294 516 1.70e-11 67.8
MS.gene70003.t1 MTR_5g075100 27.237 257 161 9 366 610 45 287 1.93e-11 67.4
MS.gene70003.t1 MTR_3g096230 26.582 237 154 7 386 616 294 516 2.13e-11 67.8
MS.gene70003.t1 MTR_3g098070 27.622 286 158 15 344 601 23 287 2.93e-11 66.2
MS.gene70003.t1 MTR_8g095440 24.468 282 172 10 344 601 24 288 4.27e-11 66.2
MS.gene70003.t1 MTR_8g100085 23.770 244 155 10 363 590 150 378 5.17e-11 65.9
MS.gene70003.t1 MTR_4g067160 29.224 219 113 10 405 600 526 725 5.46e-11 66.6
MS.gene70003.t1 MTR_1g096490 26.087 276 163 10 350 601 30 288 7.52e-11 65.5
MS.gene70003.t1 MTR_3g098070 27.083 288 157 14 344 601 23 287 9.27e-11 65.5
MS.gene70003.t1 MTR_1g026190 23.701 308 187 12 349 631 77 361 9.49e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70003.t1 AT2G17520 50.920 326 118 8 336 620 399 723 3.92e-93 308
MS.gene70003.t1 AT5G24360 50.625 320 118 7 338 620 447 763 3.15e-91 304
MS.gene70003.t1 AT5G24360 50.625 320 118 7 338 620 433 749 4.25e-91 303
MS.gene70003.t1 AT5G24360 50.625 320 118 7 338 620 453 769 5.34e-91 303
MS.gene70003.t1 AT3G11870 39.623 318 149 15 343 620 113 427 2.11e-47 176
MS.gene70003.t1 AT4G19110 26.441 295 155 12 354 603 8 285 2.47e-18 89.0
MS.gene70003.t1 AT4G19110 26.441 295 155 12 354 603 8 285 2.80e-18 89.0
MS.gene70003.t1 AT5G66210 28.467 274 155 14 364 615 82 336 4.51e-15 78.6
MS.gene70003.t1 AT5G66210 28.467 274 155 14 364 615 82 336 4.51e-15 78.6
MS.gene70003.t1 AT5G18700 30.097 206 113 7 356 547 10 198 6.81e-15 79.3
MS.gene70003.t1 AT5G66210 28.467 274 155 14 364 615 82 336 8.22e-15 78.2
MS.gene70003.t1 AT5G66210 28.467 274 155 14 364 615 82 336 8.40e-15 78.2
MS.gene70003.t1 AT5G66850 26.568 271 165 10 386 642 396 646 9.48e-15 78.6
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 9.64e-15 78.2
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 9.64e-15 78.2
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 9.90e-15 78.2
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 1.02e-14 78.2
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 1.03e-14 78.2
MS.gene70003.t1 AT3G06030 27.778 252 144 8 371 606 106 335 1.06e-14 78.2
MS.gene70003.t1 AT1G53570 28.448 232 147 6 386 612 264 481 1.06e-14 78.2
MS.gene70003.t1 AT5G66850 26.568 271 165 10 386 642 396 646 1.31e-14 78.2
MS.gene70003.t1 AT5G66210 28.000 275 155 14 364 615 82 336 1.40e-14 77.4
MS.gene70003.t1 AT5G66210 28.000 275 155 14 364 615 82 336 1.40e-14 77.4
MS.gene70003.t1 AT3G61960 29.070 258 154 10 364 606 30 273 4.11e-14 76.3
MS.gene70003.t1 AT3G61960 29.070 258 154 10 364 606 30 273 4.38e-14 76.3
MS.gene70003.t1 AT5G12180 28.333 240 138 9 395 620 131 350 1.16e-13 74.7
MS.gene70003.t1 AT3G53930 27.491 291 166 12 340 605 11 281 1.18e-13 75.1
MS.gene70003.t1 AT2G41140 26.688 311 180 15 325 606 99 390 1.19e-13 74.7
MS.gene70003.t1 AT3G53930 27.491 291 166 12 340 605 11 281 1.26e-13 75.1
MS.gene70003.t1 AT2G37840 27.778 288 163 13 343 605 6 273 1.68e-13 74.7
MS.gene70003.t1 AT5G19360 27.917 240 139 9 395 620 126 345 1.88e-13 73.9
MS.gene70003.t1 AT3G48750 26.531 294 160 13 354 603 8 289 2.19e-13 72.0
MS.gene70003.t1 AT3G49370 28.358 268 152 12 368 615 169 416 2.63e-13 73.6
MS.gene70003.t1 AT3G56760 26.129 310 183 13 325 606 100 391 3.20e-13 73.6
MS.gene70003.t1 AT5G24430 26.537 309 175 14 336 615 132 417 4.06e-13 73.2
MS.gene70003.t1 AT1G09000 26.255 259 141 8 371 607 107 337 6.03e-13 72.8
MS.gene70003.t1 AT3G54180 29.064 203 108 7 439 606 105 306 7.19e-13 70.5
MS.gene70003.t1 AT1G09000 26.357 258 140 8 371 606 107 336 8.44e-13 72.4
MS.gene70003.t1 AT5G57630 31.628 215 124 9 343 551 15 212 1.28e-12 70.9
MS.gene70003.t1 AT5G57630 31.628 215 124 9 343 551 15 212 1.37e-12 70.9
MS.gene70003.t1 AT3G45240 26.299 308 168 10 335 609 93 374 1.41e-12 70.5
MS.gene70003.t1 AT4G12020 31.390 223 122 11 387 600 1676 1876 1.43e-12 72.0
MS.gene70003.t1 AT4G12020 31.390 223 122 11 387 600 1601 1801 1.50e-12 72.0
MS.gene70003.t1 AT4G12020 31.390 223 122 11 387 600 1668 1868 1.61e-12 72.0
MS.gene70003.t1 AT4G04710 27.451 255 164 10 355 603 41 280 1.65e-12 70.9
MS.gene70003.t1 AT2G38620 28.139 231 129 8 411 606 18 246 2.09e-12 68.2
MS.gene70003.t1 AT4G04710 27.848 237 154 8 373 603 69 294 3.20e-12 70.1
MS.gene70003.t1 AT1G50700 25.180 278 167 10 349 602 72 332 3.79e-12 69.7
MS.gene70003.t1 AT1G50700 25.180 278 167 10 349 602 72 332 4.28e-12 69.7
MS.gene70003.t1 AT4G04720 24.528 265 167 9 354 605 98 342 4.90e-12 69.7
MS.gene70003.t1 AT4G04720 24.528 265 167 9 354 605 98 342 4.90e-12 69.7
MS.gene70003.t1 AT3G45240 25.879 313 166 11 335 609 93 377 5.41e-12 68.9
MS.gene70003.t1 AT3G45240 25.879 313 166 11 335 609 93 377 5.41e-12 68.9
MS.gene70003.t1 AT1G76040 26.165 279 159 14 354 605 116 374 5.61e-12 69.3
MS.gene70003.t1 AT1G50700 25.180 278 167 10 349 602 72 332 5.63e-12 69.3
MS.gene70003.t1 AT1G76040 25.784 287 166 14 346 605 108 374 6.90e-12 68.9
MS.gene70003.t1 AT4G04695 26.071 280 178 11 346 605 24 294 1.20e-11 68.2
MS.gene70003.t1 AT2G46700 23.871 310 192 11 325 606 117 410 1.85e-11 67.8
MS.gene70003.t1 AT1G14000 26.667 270 163 10 350 597 162 418 2.73e-11 66.6
MS.gene70003.t1 AT5G21326 27.987 318 174 16 351 636 10 304 3.89e-11 66.2
MS.gene70003.t1 AT1G49580 28.159 277 154 14 354 602 154 413 4.10e-11 66.6
MS.gene70003.t1 AT4G29810 27.074 229 140 9 386 601 116 330 5.24e-11 65.5
MS.gene70003.t1 AT5G39440 28.102 274 165 13 367 626 42 297 5.40e-11 66.2
MS.gene70003.t1 AT4G26890 29.057 265 157 11 356 606 11 258 5.57e-11 65.9
MS.gene70003.t1 AT4G29810 27.074 229 140 9 386 601 125 339 6.13e-11 65.5
MS.gene70003.t1 AT2G30360 27.341 267 154 10 364 610 41 287 9.66e-11 65.1

Find 148 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACGTCGGGATCAGGATTT+TGG 0.142257 8.1:-33012559 MS.gene70003:CDS
AGCATACAAAACTCATTCTA+AGG 0.255054 8.1:-33009573 MS.gene70003:CDS
CAACTTAGGGGTATTGTTGC+AGG 0.255348 8.1:-33011560 MS.gene70003:intron
TTGTTGAAAAGGAAAATATT+AGG 0.257697 8.1:-33009763 MS.gene70003:CDS
TCAATTCCTTGATGCTGCTC+TGG 0.258221 8.1:+33011020 None:intergenic
CAATGATCATGATGGTAATA+TGG 0.262900 8.1:-33012279 MS.gene70003:CDS
AAACTCTCTTCTGGGAATTT+CGG 0.275762 8.1:+33009855 None:intergenic
TTTGTCAAGTATGGTGTAAA+AGG 0.281976 8.1:+33009620 None:intergenic
TATAATGGAGAAGCTGTAAT+TGG 0.296066 8.1:-33012733 MS.gene70003:CDS
TTCTGCATATCATGAGTTAA+AGG 0.297925 8.1:+33009659 None:intergenic
TACTTGCAACTTAAATGATT+TGG 0.298869 8.1:-33011765 MS.gene70003:CDS
TGATGTTCCTTGACCACAAT+TGG 0.301365 8.1:+33009788 None:intergenic
CGCTGCTGATCAATATTTAT+AGG 0.306460 8.1:-33012610 MS.gene70003:CDS
CAATTCCTTGATGCTGCTCT+GGG 0.308125 8.1:+33011021 None:intergenic
CATTTGGAGATGATCATCTT+TGG 0.309328 8.1:-33010929 MS.gene70003:CDS
CAATGATTTACCTTAGATTC+TGG 0.314537 8.1:+33010811 None:intergenic
CCTATCATAATTTCATATAA+TGG 0.329824 8.1:-33012748 MS.gene70003:CDS
GCTACAGACATATTCAGTTT+AGG 0.344088 8.1:-33010990 MS.gene70003:CDS
CCAACCTGTACAGTAGGATT+TGG 0.344486 8.1:+33011049 None:intergenic
CCATTATATGAAATTATGAT+AGG 0.350325 8.1:+33012748 None:intergenic
TGTTGAAAAGGAAAATATTA+GGG 0.360331 8.1:-33009762 MS.gene70003:CDS
AGGATACTACAGAATATCTT+TGG 0.362857 8.1:-33011724 MS.gene70003:CDS
AACTTTAATGAAGGTGTTGA+TGG 0.366845 8.1:-33012013 MS.gene70003:CDS
TCGAAAAGATCTTTCCTTAG+TGG 0.367434 8.1:-33010883 MS.gene70003:CDS
GTAGATGGCCACAAAATAAA+TGG 0.373510 8.1:+33013192 None:intergenic
ACTTCTATATTATTATCCTT+TGG 0.374652 8.1:+33012043 None:intergenic
TAAACCCTGACCAGAATCTA+AGG 0.378020 8.1:-33010821 MS.gene70003:intron
AGAAGCCGCTTGACTGCAAC+TGG 0.379342 8.1:+33011914 None:intergenic
TACTGCTATCTCGTGGTAAA+GGG 0.383720 8.1:-33012085 MS.gene70003:CDS
TTCCTTAGTGGAGTTCATTC+CGG 0.386781 8.1:-33010871 MS.gene70003:CDS
CATGGGACCATTCTCGTAGA+TGG 0.392921 8.1:+33013177 None:intergenic
TTGCGTGTCCACAACATCAT+TGG 0.395607 8.1:+33009730 None:intergenic
GATTTGGTTCAAGTGGAGTC+AGG 0.397235 8.1:-33011749 MS.gene70003:CDS
CTAATAAGATCCTCAGCTTC+CGG 0.400149 8.1:+33010852 None:intergenic
GGAACATCAGATTGTTGAAA+AGG 0.408020 8.1:-33009774 MS.gene70003:CDS
GGCATTAACAAACATGTTCC+TGG 0.414027 8.1:-33011434 MS.gene70003:intron
ACCTTCCATTCGTCCTTCCC+TGG 0.420513 8.1:+33011948 None:intergenic
CTACTGCTATCTCGTGGTAA+AGG 0.421446 8.1:-33012086 MS.gene70003:CDS
ACTAAGGGAAGCCATCCATT+TGG 0.421454 8.1:-33010945 MS.gene70003:CDS
GATGGTCCCACGTCGGGATC+AGG 0.425123 8.1:-33012566 MS.gene70003:CDS
CGTTGAGGTTTCACCTGATC+AGG 0.426933 8.1:-33009705 MS.gene70003:CDS
CCATGCCATTTGACAATGTT+CGG 0.427560 8.1:+33011833 None:intergenic
ATTTACCTTAGATTCTGGTC+AGG 0.428433 8.1:+33010816 None:intergenic
GTCAGAAAGCTTTACACATA+AGG 0.445083 8.1:+33011460 None:intergenic
AGTTCACATGGTGCTTATCA+TGG 0.451030 8.1:-33012377 MS.gene70003:CDS
GAGGAAGAGGAACAGAAACC+AGG 0.453094 8.1:+33013471 None:intergenic
AAACTGTTTGCCGCACCTGA+TGG 0.455756 8.1:-33013217 MS.gene70003:CDS
CTTAAATGATTTGGTTCAAG+TGG 0.456881 8.1:-33011756 MS.gene70003:CDS
AACTGTTTGCCGCACCTGAT+GGG 0.457845 8.1:-33013216 MS.gene70003:CDS
ACCTGCTCTTGTACCTGATC+AGG 0.458556 8.1:+33009692 None:intergenic
AACTTAGGGGTATTGTTGCA+GGG 0.461822 8.1:-33011559 MS.gene70003:intron
TTCGCCAAATCCTACTGTAC+AGG 0.462671 8.1:-33011053 MS.gene70003:CDS
GCTTGATACGAGCTGTAGAT+TGG 0.462728 8.1:+33013124 None:intergenic
TAGTATGTTCGTTATTCTGT+TGG 0.467334 8.1:-33012122 MS.gene70003:CDS
AAAGGAAAATATTAGGGTTG+AGG 0.475152 8.1:-33009756 MS.gene70003:CDS
GGTGTTGATGGAGAAATAAT+TGG 0.476448 8.1:-33012001 MS.gene70003:CDS
AAGTATGATGGTCCCACGTC+GGG 0.481203 8.1:-33012572 MS.gene70003:CDS
TTTGTGGCCATCTACGAGAA+TGG 0.482936 8.1:-33013184 MS.gene70003:CDS
GGATAAGTACTACGGCAAAC+TGG 0.485253 8.1:-33013047 MS.gene70003:intron
ACAAAATAAATGGTCCCATC+AGG 0.485253 8.1:+33013202 None:intergenic
CATCTCCAACGCTGAAATGA+AGG 0.486389 8.1:-33009544 None:intergenic
GATGTTCCTTGACCACAATT+GGG 0.486905 8.1:+33009789 None:intergenic
AATTAGTTCCGTCTTGTCAC+TGG 0.487641 8.1:+33009817 None:intergenic
AAAAGATGAAAGTTCTCCCC+TGG 0.489626 8.1:-33013489 None:intergenic
ACCTGATCAGGTACAAGAGC+AGG 0.499859 8.1:-33009693 MS.gene70003:CDS
ATTTGACAATGTTCGGATGA+TGG 0.500705 8.1:+33011840 None:intergenic
CTTCATTTCAGCGTTGGAGA+TGG 0.502144 8.1:+33009545 None:intergenic
TTTGATGCATTTAGATTCCC+AGG 0.505866 8.1:+33012635 None:intergenic
TGCTTCTTTAGACATGGGTA+AGG 0.508181 8.1:+33012452 None:intergenic
AGAACGGACAGAGGAGGAGG+AGG 0.510525 8.1:+33013412 None:intergenic
TGCAACACATCAATTGCGTT+CGG 0.511163 8.1:+33009890 None:intergenic
GTTAGAACGGACAGAGGAGG+AGG 0.512450 8.1:+33013409 None:intergenic
ATGGTCCCATGAGGATATTA+TGG 0.514333 8.1:-33013165 MS.gene70003:CDS
ATCATCCGAACATTGTCAAA+TGG 0.518090 8.1:-33011838 MS.gene70003:CDS
CAAATCCTACTGTACAGGTT+GGG 0.523128 8.1:-33011048 MS.gene70003:CDS
TACAGCTCGTATCAAGCTCC+CGG 0.524482 8.1:-33013118 MS.gene70003:CDS
GGGAACCTTCATTTCAGCGT+TGG 0.528357 8.1:+33009539 None:intergenic
CCAAATCCTACTGTACAGGT+TGG 0.530465 8.1:-33011049 MS.gene70003:CDS
AGGACGAATGGAAGGTATGA+TGG 0.531409 8.1:-33011941 MS.gene70003:CDS
GCTGATCAATATTTATAGGA+AGG 0.534128 8.1:-33012606 MS.gene70003:CDS
GTTGTTCATTTGCATAAACT+AGG 0.535949 8.1:-33011536 MS.gene70003:CDS
ATGTGTAAAGCTTTCTGACA+TGG 0.538576 8.1:-33011456 MS.gene70003:CDS
GAATGACCATAATATCCTCA+TGG 0.539079 8.1:+33013159 None:intergenic
CCGAACATTGTCAAATGGCA+TGG 0.540381 8.1:-33011833 MS.gene70003:CDS
TGATGACCCAATTGTGGTCA+AGG 0.540711 8.1:-33009795 MS.gene70003:CDS
AGTTTAAGTAGTAGAGGTGA+TGG 0.542980 8.1:+33011686 None:intergenic
ATTGGCCAGGGAAGGACGAA+TGG 0.544414 8.1:-33011953 MS.gene70003:CDS
TTTACCTTAGATTCTGGTCA+GGG 0.545376 8.1:+33010817 None:intergenic
AACAGAATAACGAACATACT+AGG 0.547666 8.1:+33012124 None:intergenic
TACAACAAAATAATTACAAG+AGG 0.548877 8.1:-33012235 MS.gene70003:CDS
TGTTGTAGAAAAGGCGTAGG+AGG 0.551767 8.1:+33012251 None:intergenic
TGAATCATCATATCTGAGGC+AGG 0.554368 8.1:+33012329 None:intergenic
TGTGTAAAGCTTTCTGACAT+GGG 0.554737 8.1:-33011455 MS.gene70003:CDS
ATTTGGTTCAAGTGGAGTCA+GGG 0.557887 8.1:-33011748 MS.gene70003:CDS
AGGAAGAGGAACAGAAACCA+GGG 0.557972 8.1:+33013472 None:intergenic
TGGGACCATCATACTTCAGA+TGG 0.558649 8.1:+33012579 None:intergenic
TTCCGGAATGAACTCCACTA+AGG 0.560655 8.1:+33010869 None:intergenic
CAAAGATGATCATCTCCAAA+TGG 0.562429 8.1:+33010930 None:intergenic
CTCATCAGTTTAAGTAGTAG+AGG 0.562816 8.1:+33011680 None:intergenic
CTCTACTACTTAAACTGATG+AGG 0.562912 8.1:-33011679 MS.gene70003:intron
ACTTAGGGGTATTGTTGCAG+GGG 0.563653 8.1:-33011558 MS.gene70003:intron
CATGAAATGTAGTATGATGA+TGG 0.565289 8.1:+33012498 None:intergenic
GATTTCCATCTGAAGTATGA+TGG 0.568462 8.1:-33012584 MS.gene70003:CDS
GGATGATTGGTCATTGTACA+AGG 0.571953 8.1:-33013071 MS.gene70003:CDS
GATGATCATCTCCAAATGGA+TGG 0.581367 8.1:+33010934 None:intergenic
GGTTCAAGTGGAGTCAGGGA+AGG 0.585224 8.1:-33011744 MS.gene70003:CDS
GCCAGGGAAGGACGAATGGA+AGG 0.586542 8.1:-33011949 MS.gene70003:CDS
ATATTATGGTCATTCTCCAC+AGG 0.591103 8.1:-33013151 MS.gene70003:CDS
CGAAATCAGAACACTTATGT+CGG 0.592650 8.1:-33011864 MS.gene70003:CDS
GTCAACCAGTTGCAGTCAAG+CGG 0.593335 8.1:-33011919 MS.gene70003:CDS
GCATACAAAACTCATTCTAA+GGG 0.594060 8.1:-33009572 MS.gene70003:CDS
GTATCGATGAACTTTAATGA+AGG 0.598118 8.1:-33012022 MS.gene70003:CDS
AAGTACAACGCTCCAGTGCA+AGG 0.598602 8.1:+33011783 None:intergenic
TGAGGAATCCAATGATGTTG+TGG 0.601179 8.1:-33009738 MS.gene70003:CDS
TAAGTAGTAGAGGTGATGGA+TGG 0.602877 8.1:+33011690 None:intergenic
TACAAGGAGGATAAGTACTA+CGG 0.605030 8.1:-33013055 MS.gene70003:CDS
ATAACGAACATACTAGGTGA+TGG 0.607629 8.1:+33012130 None:intergenic
GGCTTCCCAACCTGTACAGT+AGG 0.608756 8.1:+33011043 None:intergenic
ACTAATTGATGACCCAATTG+TGG 0.609257 8.1:-33009801 MS.gene70003:CDS
GCTGTAGATTGGCGATCCTG+TGG 0.610761 8.1:+33013135 None:intergenic
TCATACTACATTTCATGTAG+AGG 0.611755 8.1:-33012492 MS.gene70003:CDS
TGTGGACACGCAACACGTTG+AGG 0.612887 8.1:-33009720 MS.gene70003:CDS
AATGACCATAATATCCTCAT+GGG 0.614134 8.1:+33013160 None:intergenic
ACTTGACACCAGTGACAAGA+CGG 0.614987 8.1:-33009825 MS.gene70003:CDS
TAATAATACAAAATCAAACT+CGG 0.615612 8.1:+33012523 None:intergenic
CATTGTCAAATGGCATGGAG+TGG 0.616032 8.1:-33011828 MS.gene70003:CDS
AACATACTAGGTGATGGAGT+TGG 0.616054 8.1:+33012136 None:intergenic
TTTGAACAAGAAGAGTGGGA+TGG 0.617852 8.1:-33012159 MS.gene70003:intron
CCAAAATCCTGATCCCGACG+TGG 0.618016 8.1:+33012559 None:intergenic
TAAGTATAAGTATATACCAA+AGG 0.620241 8.1:-33012059 MS.gene70003:CDS
GAAGCCCCAGAGCAGCATCA+AGG 0.621107 8.1:-33011026 MS.gene70003:CDS
GGAGTTCATTCCGGAAGCTG+AGG 0.621667 8.1:-33010862 MS.gene70003:CDS
CAACTGAATCATCATATCTG+AGG 0.628170 8.1:+33012325 None:intergenic
CTATAAATATTGATCAGCAG+CGG 0.630252 8.1:+33012611 None:intergenic
GTTGTTAGAACGGACAGAGG+AGG 0.630566 8.1:+33013406 None:intergenic
ACTGCTATCTCGTGGTAAAG+GGG 0.632547 8.1:-33012084 MS.gene70003:CDS
AATTCCTTGATGCTGCTCTG+GGG 0.641432 8.1:+33011022 None:intergenic
AGTAAATCACTCTCAGTGAG+AGG 0.642560 8.1:+33012194 None:intergenic
GAAGTATGATGGTCCCACGT+CGG 0.643813 8.1:-33012573 MS.gene70003:CDS
GGAAGAGGAACAGAAACCAG+GGG 0.651682 8.1:+33013473 None:intergenic
ATAAATGGTCCCATCAGGTG+CGG 0.657978 8.1:+33013207 None:intergenic
TTAATCAGTGAAAGTTCACA+TGG 0.660628 8.1:-33012389 MS.gene70003:CDS
TTCACTCTACTGCTATCTCG+TGG 0.664124 8.1:-33012092 MS.gene70003:CDS
TCATGATATGCAGAACAATG+AGG 0.681901 8.1:-33009651 MS.gene70003:CDS
CAAAATCCTGATCCCGACGT+GGG 0.684562 8.1:+33012560 None:intergenic
CATAAACTAGGAATAATACA+CGG 0.689054 8.1:-33011524 MS.gene70003:CDS
TGATTGGTCATTGTACAAGG+AGG 0.729224 8.1:-33013068 MS.gene70003:CDS
TCTACGAGAATGGTCCCATG+AGG 0.737091 8.1:-33013174 MS.gene70003:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTCATAACATTAAT+AGG + chr8.1:33012365-33012384 None:intergenic 10.0%
!! TTGAAAATAAAACAAAAATT+AGG + chr8.1:33011787-33011806 None:intergenic 10.0%
!!! AAATTAGTTTTTATATTACA+TGG - chr8.1:33012835-33012854 MS.gene70003:intron 10.0%
!!! GTAATATAAAAACTAATTTT+TGG + chr8.1:33012834-33012853 None:intergenic 10.0%
!!! TTTATAAAAAGTTTAAAGTT+CGG - chr8.1:33012677-33012696 MS.gene70003:intron 10.0%
!! AGATTAATGTTATCAAAATA+GGG + chr8.1:33012408-33012427 None:intergenic 15.0%
!! TAAACAATATAAAGAGAAAA+AGG + chr8.1:33011942-33011961 None:intergenic 15.0%
!! TAATAATACAAAATCAAACT+CGG + chr8.1:33010511-33010530 None:intergenic 15.0%
!! TAGATTAATGTTATCAAAAT+AGG + chr8.1:33012409-33012428 None:intergenic 15.0%
!! TTGAAAAGAAAACAAAAATT+AGG + chr8.1:33011846-33011865 None:intergenic 15.0%
!!! ATTTTTTTTTTGAGGAAATT+TGG - chr8.1:33010185-33010204 MS.gene70003:intron 15.0%
!!! GTAGTTTTATTTGTTTTTAT+TGG - chr8.1:33012431-33012450 MS.gene70003:CDS 15.0%
!!! TAATTATTTTGTTGTAGAAA+AGG + chr8.1:33010792-33010811 None:intergenic 15.0%
!!! TATAATAATAAGAATTTTGC+TGG - chr8.1:33010372-33010391 MS.gene70003:intron 15.0%
!!! TTTTTTTTTTGAGGAAATTT+GGG - chr8.1:33010186-33010205 MS.gene70003:intron 15.0%
!! ACTTCTATATTATTATCCTT+TGG + chr8.1:33010991-33011010 None:intergenic 20.0%
!! AGACTAAGATTTATTAGAAT+AGG - chr8.1:33012487-33012506 MS.gene70003:CDS 20.0%
!! ATTCTAATAAATCTTAGTCT+TGG + chr8.1:33012487-33012506 None:intergenic 20.0%
!! CCATTATATGAAATTATGAT+AGG + chr8.1:33010286-33010305 None:intergenic 20.0%
!! CCTATCATAATTTCATATAA+TGG - chr8.1:33010283-33010302 MS.gene70003:intron 20.0%
!! CTCTTTATATTGTTTAAACA+GGG - chr8.1:33011946-33011965 MS.gene70003:CDS 20.0%
!! CTTTATCAATTATATATGCT+AGG - chr8.1:33009664-33009683 MS.gene70003:CDS 20.0%
!! TAAGTATAAGTATATACCAA+AGG - chr8.1:33010972-33010991 MS.gene70003:CDS 20.0%
!! TACAACAAAATAATTACAAG+AGG - chr8.1:33010796-33010815 MS.gene70003:intron 20.0%
!! TAGAACATTGTAATTGTAAT+TGG - chr8.1:33010014-33010033 MS.gene70003:intron 20.0%
!! TATGTTGTATCAAATGATTA+AGG - chr8.1:33012318-33012337 MS.gene70003:CDS 20.0%
!! TCTCTTTATATTGTTTAAAC+AGG - chr8.1:33011945-33011964 MS.gene70003:CDS 20.0%
!! TGCTTATGTAAATTACAATA+TGG - chr8.1:33011700-33011719 MS.gene70003:CDS 20.0%
!! TGTTGAAAAGGAAAATATTA+GGG - chr8.1:33013269-33013288 MS.gene70003:intron 20.0%
!! TTGAAAAAACTATCAAAATC+AGG + chr8.1:33010735-33010754 None:intergenic 20.0%
!! TTGTTGAAAAGGAAAATATT+AGG - chr8.1:33013268-33013287 MS.gene70003:intron 20.0%
!!! AAATTATTTCCTTTATTTCC+AGG - chr8.1:33013118-33013137 MS.gene70003:CDS 20.0%
!!! CTTGATTTTCCTATTTATTT+AGG - chr8.1:33012930-33012949 MS.gene70003:intron 20.0%
!!! GTTATATCTTATCTTTTGAA+GGG - chr8.1:33012650-33012669 MS.gene70003:CDS 20.0%
!!! TCAAAATGTTCTAAAAATCA+AGG - chr8.1:33011542-33011561 MS.gene70003:CDS 20.0%
!!! TGTTATATCTTATCTTTTGA+AGG - chr8.1:33012649-33012668 MS.gene70003:CDS 20.0%
!!! TTGATTTTTAGAACATTTTG+AGG + chr8.1:33011543-33011562 None:intergenic 20.0%
!!! TTTTTTTTCTGTTTGACTAA+GGG - chr8.1:33012071-33012090 MS.gene70003:CDS 20.0%
!!! TTTTTTTTTCTGTTTGACTA+AGG - chr8.1:33012070-33012089 MS.gene70003:CDS 20.0%
! AAGAAAAAGAGTTGTTAGAA+CGG + chr8.1:33009638-33009657 None:intergenic 25.0%
! AAGATAAGATATAACAACCA+AGG + chr8.1:33012645-33012664 None:intergenic 25.0%
! ACACGATTATAAACTAATCT+TGG + chr8.1:33012797-33012816 None:intergenic 25.0%
! CACGATTATAAACTAATCTT+GGG + chr8.1:33012796-33012815 None:intergenic 25.0%
! CATAAACTAGGAATAATACA+CGG - chr8.1:33011507-33011526 MS.gene70003:CDS 25.0%
! CATAACATTAATAGGTATGT+TGG + chr8.1:33012357-33012376 None:intergenic 25.0%
! TAAAAATGAAACTCTCTTCT+GGG + chr8.1:33013187-33013206 None:intergenic 25.0%
! TAAATCATTGTCATTCATTG+TGG - chr8.1:33012233-33012252 MS.gene70003:CDS 25.0%
! TACTTGCAACTTAAATGATT+TGG - chr8.1:33011266-33011285 MS.gene70003:intron 25.0%
! TGATTTACTTTCTACCAATT+TGG + chr8.1:33013013-33013032 None:intergenic 25.0%
! TTCGTTTCAAAAAAAGAGAT+TGG - chr8.1:33011060-33011079 MS.gene70003:CDS 25.0%
! TTTATTTGAACAAGAAGAGT+GGG - chr8.1:33010868-33010887 MS.gene70003:CDS 25.0%
! TTTCCTATTTATTTAGGAGT+AGG - chr8.1:33012936-33012955 MS.gene70003:intron 25.0%
!! TTCTATATCAATTAGCTCTT+AGG - chr8.1:33011812-33011831 MS.gene70003:CDS 25.0%
!!! AGTTTTTTCAATGATCATGA+TGG - chr8.1:33010744-33010763 MS.gene70003:intron 25.0%
!!! ATTTTTGTTTTGCAACTTAG+GGG - chr8.1:33011459-33011478 MS.gene70003:CDS 25.0%
!!! CATTTTTGTTTTGCAACTTA+GGG - chr8.1:33011458-33011477 MS.gene70003:CDS 25.0%
!!! TTTAGTCTTTTGTACCAAAT+TGG - chr8.1:33012996-33013015 MS.gene70003:intron 25.0%
!!! TTTTGTTTCTTTGTCAAGTA+TGG + chr8.1:33013423-33013442 None:intergenic 25.0%
AAAGGAAAATATTAGGGTTG+AGG - chr8.1:33013275-33013294 MS.gene70003:intron 30.0%
AACAGAATAACGAACATACT+AGG + chr8.1:33010910-33010929 None:intergenic 30.0%
AACATTAGATTCGATCGATA+TGG + chr8.1:33011877-33011896 None:intergenic 30.0%
AATGACCATAATATCCTCAT+GGG + chr8.1:33009874-33009893 None:intergenic 30.0%
ACATTCACAAGTTAAATGCT+AGG + chr8.1:33011657-33011676 None:intergenic 30.0%
AGCATACAAAACTCATTCTA+AGG - chr8.1:33013458-33013477 MS.gene70003:CDS 30.0%
AGGATACTACAGAATATCTT+TGG - chr8.1:33011307-33011326 MS.gene70003:intron 30.0%
CAATGATCATGATGGTAATA+TGG - chr8.1:33010752-33010771 MS.gene70003:intron 30.0%
CAATGATTTACCTTAGATTC+TGG + chr8.1:33012223-33012242 None:intergenic 30.0%
CATGAAATGTAGTATGATGA+TGG + chr8.1:33010536-33010555 None:intergenic 30.0%
CCTAAGTTGCAAAACAAAAA+TGG + chr8.1:33011460-33011479 None:intergenic 30.0%
CGATCGAATCTAATGTTATT+CGG - chr8.1:33011880-33011899 MS.gene70003:CDS 30.0%
CTATAAATATTGATCAGCAG+CGG + chr8.1:33010423-33010442 None:intergenic 30.0%
GAGAAAAAGGTCAATGAATT+TGG + chr8.1:33011929-33011948 None:intergenic 30.0%
GCATACAAAACTCATTCTAA+GGG - chr8.1:33013459-33013478 MS.gene70003:CDS 30.0%
GCTGATCAATATTTATAGGA+AGG - chr8.1:33010425-33010444 MS.gene70003:intron 30.0%
GTAAAAATGAAACTCTCTTC+TGG + chr8.1:33013188-33013207 None:intergenic 30.0%
GTTGTTCATTTGCATAAACT+AGG - chr8.1:33011495-33011514 MS.gene70003:CDS 30.0%
GTTTATTTGAACAAGAAGAG+TGG - chr8.1:33010867-33010886 MS.gene70003:CDS 30.0%
TAAAAATCTTAGTGCTTACC+AGG + chr8.1:33011618-33011637 None:intergenic 30.0%
TAGTATGTTCGTTATTCTGT+TGG - chr8.1:33010909-33010928 MS.gene70003:CDS 30.0%
TCATACTACATTTCATGTAG+AGG - chr8.1:33010539-33010558 MS.gene70003:intron 30.0%
TTAATCAGTGAAAGTTCACA+TGG - chr8.1:33010642-33010661 MS.gene70003:intron 30.0%
TTCTGCATATCATGAGTTAA+AGG + chr8.1:33013375-33013394 None:intergenic 30.0%
TTTAACTCTCTAGCTAATGT+AGG + chr8.1:33010074-33010093 None:intergenic 30.0%
TTTGTCAAGTATGGTGTAAA+AGG + chr8.1:33013414-33013433 None:intergenic 30.0%
! ACTTTTTGCTTCTTTAGACA+TGG + chr8.1:33010588-33010607 None:intergenic 30.0%
! CTTTTTGCTTCTTTAGACAT+GGG + chr8.1:33010587-33010606 None:intergenic 30.0%
! GTATCGATGAACTTTAATGA+AGG - chr8.1:33011009-33011028 MS.gene70003:CDS 30.0%
! TATAATGGAGAAGCTGTAAT+TGG - chr8.1:33010298-33010317 MS.gene70003:intron 30.0%
!! AACTTTAATGAAGGTGTTGA+TGG - chr8.1:33011018-33011037 MS.gene70003:CDS 30.0%
!! AGATTTTATTTACCTTGCAC+TGG - chr8.1:33011236-33011255 MS.gene70003:intron 30.0%
!! AGCTTTAGAGTTTAGATTGA+AGG - chr8.1:33012702-33012721 MS.gene70003:intron 30.0%
!! CTTAAATGATTTGGTTCAAG+TGG - chr8.1:33011275-33011294 MS.gene70003:intron 30.0%
!!! CCATTTTTGTTTTGCAACTT+AGG - chr8.1:33011457-33011476 MS.gene70003:CDS 30.0%
AATCCCATGCTAGACATAAT+TGG + chr8.1:33011680-33011699 None:intergenic 35.0%
ACAAAATAAATGGTCCCATC+AGG + chr8.1:33009832-33009851 None:intergenic 35.0%
ACTTACAATGTGCACAAGTA+AGG + chr8.1:33011400-33011419 None:intergenic 35.0%
AGTTTAAGTAGTAGAGGTGA+TGG + chr8.1:33011348-33011367 None:intergenic 35.0%
ATAACGAACATACTAGGTGA+TGG + chr8.1:33010904-33010923 None:intergenic 35.0%
ATATTATGGTCATTCTCCAC+AGG - chr8.1:33009880-33009899 MS.gene70003:CDS 35.0%
ATCATCCGAACATTGTCAAA+TGG - chr8.1:33011193-33011212 MS.gene70003:intron 35.0%
ATGTCCAATTATGTCTAGCA+TGG - chr8.1:33011673-33011692 MS.gene70003:intron 35.0%
ATGTGTAAAGCTTTCTGACA+TGG - chr8.1:33011575-33011594 MS.gene70003:intron 35.0%
ATTTACCTTAGATTCTGGTC+AGG + chr8.1:33012218-33012237 None:intergenic 35.0%
ATTTATTTAGGAGTAGGAGC+AGG - chr8.1:33012942-33012961 MS.gene70003:intron 35.0%
ATTTGACAATGTTCGGATGA+TGG + chr8.1:33011194-33011213 None:intergenic 35.0%
CAAAGATGATCATCTCCAAA+TGG + chr8.1:33012104-33012123 None:intergenic 35.0%
CAACTGAATCATCATATCTG+AGG + chr8.1:33010709-33010728 None:intergenic 35.0%
CATTTGGAGATGATCATCTT+TGG - chr8.1:33012102-33012121 MS.gene70003:CDS 35.0%
CGAAATCAGAACACTTATGT+CGG - chr8.1:33011167-33011186 MS.gene70003:intron 35.0%
CGCTGCTGATCAATATTTAT+AGG - chr8.1:33010421-33010440 MS.gene70003:intron 35.0%
CTAATCTTGGGAGATAAACT+AGG + chr8.1:33012784-33012803 None:intergenic 35.0%
CTCATCAGTTTAAGTAGTAG+AGG + chr8.1:33011354-33011373 None:intergenic 35.0%
CTCTACTACTTAAACTGATG+AGG - chr8.1:33011352-33011371 MS.gene70003:intron 35.0%
CTTACAATGTGCACAAGTAA+GGG + chr8.1:33011399-33011418 None:intergenic 35.0%
GAATGACCATAATATCCTCA+TGG + chr8.1:33009875-33009894 None:intergenic 35.0%
GCTACAGACATATTCAGTTT+AGG - chr8.1:33012041-33012060 MS.gene70003:CDS 35.0%
GCTCCTACTCCTAAATAAAT+AGG + chr8.1:33012942-33012961 None:intergenic 35.0%
GGAACATCAGATTGTTGAAA+AGG - chr8.1:33013257-33013276 MS.gene70003:intron 35.0%
GTAGATGGCCACAAAATAAA+TGG + chr8.1:33009842-33009861 None:intergenic 35.0%
GTCAGAAAGCTTTACACATA+AGG + chr8.1:33011574-33011593 None:intergenic 35.0%
TACAAGGAGGATAAGTACTA+CGG - chr8.1:33009976-33009995 MS.gene70003:intron 35.0%
TCATGATATGCAGAACAATG+AGG - chr8.1:33013380-33013399 MS.gene70003:intron 35.0%
TCGAAAAGATCTTTCCTTAG+TGG - chr8.1:33012148-33012167 MS.gene70003:intron 35.0%
TGCTATTGCGTATTTCTTTC+TGG - chr8.1:33012555-33012574 MS.gene70003:CDS 35.0%
TGTCCAATTATGTCTAGCAT+GGG - chr8.1:33011674-33011693 MS.gene70003:intron 35.0%
TGTGTAAAGCTTTCTGACAT+GGG - chr8.1:33011576-33011595 MS.gene70003:intron 35.0%
TTTACCTTAGATTCTGGTCA+GGG + chr8.1:33012217-33012236 None:intergenic 35.0%
TTTGATGCATTTAGATTCCC+AGG + chr8.1:33010399-33010418 None:intergenic 35.0%
! AAACTCTCTTCTGGGAATTT+CGG + chr8.1:33013179-33013198 None:intergenic 35.0%
! AAAGATCTTTTCGATCGTTC+AGG + chr8.1:33012142-33012161 None:intergenic 35.0%
! AATAAGAATTTTGCTGGTCC+TGG - chr8.1:33010378-33010397 MS.gene70003:intron 35.0%
! ACTAATTGATGACCCAATTG+TGG - chr8.1:33013230-33013249 MS.gene70003:CDS 35.0%
! ATAAGAATTTTGCTGGTCCT+GGG - chr8.1:33010379-33010398 MS.gene70003:intron 35.0%
! ATTTGGGATTGATGTTCTGT+TGG - chr8.1:33010202-33010221 MS.gene70003:intron 35.0%
! GATTTCCATCTGAAGTATGA+TGG - chr8.1:33010447-33010466 MS.gene70003:intron 35.0%
! TCAAAAAAAGAGATTGGCCA+GGG - chr8.1:33011066-33011085 MS.gene70003:CDS 35.0%
! TTCAAAAAAAGAGATTGGCC+AGG - chr8.1:33011065-33011084 MS.gene70003:CDS 35.0%
! TTCTGGGAATTTCGGAAAAA+AGG + chr8.1:33013171-33013190 None:intergenic 35.0%
! TTTTCGATCGTTCAGGATAT+TGG + chr8.1:33012135-33012154 None:intergenic 35.0%
!! GGTGTTGATGGAGAAATAAT+TGG - chr8.1:33011030-33011049 MS.gene70003:CDS 35.0%
!! TTCAAGTTGAAGCCAAAACT+GGG - chr8.1:33010341-33010360 MS.gene70003:intron 35.0%
!! TTTATTGGTCTGCTGATTTC+AGG - chr8.1:33012446-33012465 MS.gene70003:CDS 35.0%
!! TTTCAAGTTGAAGCCAAAAC+TGG - chr8.1:33010340-33010359 MS.gene70003:intron 35.0%
!! TTTTGTTGTAGAAAAGGCGT+AGG + chr8.1:33010786-33010805 None:intergenic 35.0%
!!! GTCATTCATTGTGGTTTTAG+AGG - chr8.1:33012242-33012261 MS.gene70003:CDS 35.0%
!!! TGATGGGACCATTTATTTTG+TGG - chr8.1:33009831-33009850 MS.gene70003:CDS 35.0%
AATTAGTTCCGTCTTGTCAC+TGG + chr8.1:33013217-33013236 None:intergenic 40.0%
AGTAAATCACTCTCAGTGAG+AGG + chr8.1:33010840-33010859 None:intergenic 40.0%
AGTCTTGAAAAAGCGAGAAG+AGG + chr8.1:33009582-33009601 None:intergenic 40.0%
AGTTCACATGGTGCTTATCA+TGG - chr8.1:33010654-33010673 MS.gene70003:intron 40.0%
ATGGTCCCATGAGGATATTA+TGG - chr8.1:33009866-33009885 MS.gene70003:CDS 40.0%
CAAATCCTACTGTACAGGTT+GGG - chr8.1:33011983-33012002 MS.gene70003:CDS 40.0%
CTAATAAGATCCTCAGCTTC+CGG + chr8.1:33012182-33012201 None:intergenic 40.0%
CTCACTGAGAGTGATTTACT+AGG - chr8.1:33010840-33010859 MS.gene70003:CDS 40.0%
GATGATCATCTCCAAATGGA+TGG + chr8.1:33012100-33012119 None:intergenic 40.0%
GATGTTCCTTGACCACAATT+GGG + chr8.1:33013245-33013264 None:intergenic 40.0%
GGCATTAACAAACATGTTCC+TGG - chr8.1:33011597-33011616 MS.gene70003:intron 40.0%
GGGAGATAAACTAGGATACT+AGG + chr8.1:33012776-33012795 None:intergenic 40.0%
GTATTGCATCTGCTATGACT+TGG - chr8.1:33012287-33012306 MS.gene70003:CDS 40.0%
TAAACCCTGACCAGAATCTA+AGG - chr8.1:33012210-33012229 MS.gene70003:CDS 40.0%
TACTGCTATCTCGTGGTAAA+GGG - chr8.1:33010946-33010965 MS.gene70003:CDS 40.0%
TGAGGAATCCAATGATGTTG+TGG - chr8.1:33013293-33013312 MS.gene70003:intron 40.0%
TGATGTTCCTTGACCACAAT+TGG + chr8.1:33013246-33013265 None:intergenic 40.0%
TGCAACACATCAATTGCGTT+CGG + chr8.1:33013144-33013163 None:intergenic 40.0%
TGCTTCTTTAGACATGGGTA+AGG + chr8.1:33010582-33010601 None:intergenic 40.0%
TTCCTTAGTGGAGTTCATTC+CGG - chr8.1:33012160-33012179 MS.gene70003:intron 40.0%
TTTACCCTACTACGTGAGAT+CGG + chr8.1:33012868-33012887 None:intergenic 40.0%
TTTGAACAAGAAGAGTGGGA+TGG - chr8.1:33010872-33010891 MS.gene70003:CDS 40.0%
! ATTTGGTTCAAGTGGAGTCA+GGG - chr8.1:33011283-33011302 MS.gene70003:intron 40.0%
! CCATGCCATTTGACAATGTT+CGG + chr8.1:33011201-33011220 None:intergenic 40.0%
! GGATGATTGGTCATTGTACA+AGG - chr8.1:33009960-33009979 MS.gene70003:intron 40.0%
! TGAATCATCATATCTGAGGC+AGG + chr8.1:33010705-33010724 None:intergenic 40.0%
! TGATTGGTCATTGTACAAGG+AGG - chr8.1:33009963-33009982 MS.gene70003:intron 40.0%
! TTTTGGACTGTGAGGATGAT+TGG - chr8.1:33009947-33009966 MS.gene70003:intron 40.0%
!! AACATACTAGGTGATGGAGT+TGG + chr8.1:33010898-33010917 None:intergenic 40.0%
!! AACTTAGGGGTATTGTTGCA+GGG - chr8.1:33011472-33011491 MS.gene70003:CDS 40.0%
!! TAAGTAGTAGAGGTGATGGA+TGG + chr8.1:33011344-33011363 None:intergenic 40.0%
!! TAGCGAATTTTTGGACTGTG+AGG - chr8.1:33009939-33009958 MS.gene70003:intron 40.0%
AATTCCTTGATGCTGCTCTG+GGG + chr8.1:33012012-33012031 None:intergenic 45.0%
ACTAAGGGAAGCCATCCATT+TGG - chr8.1:33012086-33012105 MS.gene70003:CDS 45.0%
ACTGCTATCTCGTGGTAAAG+GGG - chr8.1:33010947-33010966 MS.gene70003:CDS 45.0%
ACTTGACACCAGTGACAAGA+CGG - chr8.1:33013206-33013225 MS.gene70003:CDS 45.0%
AGAGTTGTTAGAACGGACAG+AGG + chr8.1:33009631-33009650 None:intergenic 45.0%
AGGAAGAGGAACAGAAACCA+GGG + chr8.1:33009562-33009581 None:intergenic 45.0%
AGTTGAAGCCAAAACTGGGT+CGG - chr8.1:33010345-33010364 MS.gene70003:intron 45.0%
ATAAATGGTCCCATCAGGTG+CGG + chr8.1:33009827-33009846 None:intergenic 45.0%
ATTTGCCGATCTCACGTAGT+AGG - chr8.1:33012860-33012879 MS.gene70003:intron 45.0%
CAATTCCTTGATGCTGCTCT+GGG + chr8.1:33012013-33012032 None:intergenic 45.0%
CATTGTCAAATGGCATGGAG+TGG - chr8.1:33011203-33011222 MS.gene70003:intron 45.0%
CCAAATCCTACTGTACAGGT+TGG - chr8.1:33011982-33012001 MS.gene70003:CDS 45.0%
CCAACCTGTACAGTAGGATT+TGG + chr8.1:33011985-33012004 None:intergenic 45.0%
CCGAACATTGTCAAATGGCA+TGG - chr8.1:33011198-33011217 MS.gene70003:intron 45.0%
CTACTGCTATCTCGTGGTAA+AGG - chr8.1:33010945-33010964 MS.gene70003:CDS 45.0%
GAAAAAGCGAGAAGAGGAAG+AGG + chr8.1:33009576-33009595 None:intergenic 45.0%
GATCTCACGTAGTAGGGTAA+AGG - chr8.1:33012867-33012886 MS.gene70003:intron 45.0%
GCTTGATACGAGCTGTAGAT+TGG + chr8.1:33009910-33009929 None:intergenic 45.0%
GGATAAGTACTACGGCAAAC+TGG - chr8.1:33009984-33010003 MS.gene70003:intron 45.0%
GTCCAAAAATTCGCTAGCTC+CGG + chr8.1:33009935-33009954 None:intergenic 45.0%
TCAATTCCTTGATGCTGCTC+TGG + chr8.1:33012014-33012033 None:intergenic 45.0%
TCCAAAAATTCGCTAGCTCC+GGG + chr8.1:33009934-33009953 None:intergenic 45.0%
TGATGACCCAATTGTGGTCA+AGG - chr8.1:33013236-33013255 MS.gene70003:CDS 45.0%
TGGGACCATCATACTTCAGA+TGG + chr8.1:33010455-33010474 None:intergenic 45.0%
TTCACTCTACTGCTATCTCG+TGG - chr8.1:33010939-33010958 MS.gene70003:CDS 45.0%
TTCCGGAATGAACTCCACTA+AGG + chr8.1:33012165-33012184 None:intergenic 45.0%
TTCGCCAAATCCTACTGTAC+AGG - chr8.1:33011978-33011997 MS.gene70003:CDS 45.0%
TTGCGTGTCCACAACATCAT+TGG + chr8.1:33013304-33013323 None:intergenic 45.0%
TTTGCCGATCTCACGTAGTA+GGG - chr8.1:33012861-33012880 MS.gene70003:intron 45.0%
TTTGTGGCCATCTACGAGAA+TGG - chr8.1:33009847-33009866 MS.gene70003:CDS 45.0%
! AAAAAGAGATTGGCCAGGGA+AGG - chr8.1:33011070-33011089 MS.gene70003:intron 45.0%
! AGGACGAATGGAAGGTATGA+TGG - chr8.1:33011090-33011109 MS.gene70003:intron 45.0%
! GATTTGGTTCAAGTGGAGTC+AGG - chr8.1:33011282-33011301 MS.gene70003:intron 45.0%
! TATACTGACCGACCCAGTTT+TGG + chr8.1:33010356-33010375 None:intergenic 45.0%
! TGTTGTAGAAAAGGCGTAGG+AGG + chr8.1:33010783-33010802 None:intergenic 45.0%
!! ACTTAGGGGTATTGTTGCAG+GGG - chr8.1:33011473-33011492 MS.gene70003:CDS 45.0%
!! ATTGGTCTGCTGATTTCAGG+AGG - chr8.1:33012449-33012468 MS.gene70003:CDS 45.0%
!! CAACTTAGGGGTATTGTTGC+AGG - chr8.1:33011471-33011490 MS.gene70003:CDS 45.0%
!! TTTGCTGTTTGAGTCTCGTC+TGG - chr8.1:33012616-33012635 MS.gene70003:CDS 45.0%
!!! TAATATAAAAACTAATTTTT+GGG + chr8.1:33012833-33012852 None:intergenic 5.0%
!!! TTTATTTTATTTTTTTTTTG+AGG - chr8.1:33010177-33010196 MS.gene70003:intron 5.0%
AAACTGTTTGCCGCACCTGA+TGG - chr8.1:33009814-33009833 MS.gene70003:CDS 50.0%
AACTGTTTGCCGCACCTGAT+GGG - chr8.1:33009815-33009834 MS.gene70003:CDS 50.0%
AAGTACAACGCTCCAGTGCA+AGG + chr8.1:33011251-33011270 None:intergenic 50.0%
AAGTATGATGGTCCCACGTC+GGG - chr8.1:33010459-33010478 MS.gene70003:intron 50.0%
ACCTGATCAGGTACAAGAGC+AGG - chr8.1:33013338-33013357 MS.gene70003:intron 50.0%
ACCTGCTCTTGTACCTGATC+AGG + chr8.1:33013342-33013361 None:intergenic 50.0%
CAAAATCCTGATCCCGACGT+GGG + chr8.1:33010474-33010493 None:intergenic 50.0%
CATGGGACCATTCTCGTAGA+TGG + chr8.1:33009857-33009876 None:intergenic 50.0%
CGTTGAGGTTTCACCTGATC+AGG - chr8.1:33013326-33013345 MS.gene70003:intron 50.0%
GAAGTATGATGGTCCCACGT+CGG - chr8.1:33010458-33010477 MS.gene70003:intron 50.0%
GAGGAAGAGGAACAGAAACC+AGG + chr8.1:33009563-33009582 None:intergenic 50.0%
GGAAGAGGAACAGAAACCAG+GGG + chr8.1:33009561-33009580 None:intergenic 50.0%
GTCAACCAGTTGCAGTCAAG+CGG - chr8.1:33011112-33011131 MS.gene70003:intron 50.0%
GTTGTTAGAACGGACAGAGG+AGG + chr8.1:33009628-33009647 None:intergenic 50.0%
TACAGCTCGTATCAAGCTCC+CGG - chr8.1:33009913-33009932 MS.gene70003:intron 50.0%
TCTACGAGAATGGTCCCATG+AGG - chr8.1:33009857-33009876 MS.gene70003:CDS 50.0%
TGAGTCTCGTCTGGTTACCT+TGG - chr8.1:33012625-33012644 MS.gene70003:CDS 50.0%
TGCGTTCGGCCTGGAAATAA+AGG + chr8.1:33013130-33013149 None:intergenic 50.0%
! TCCCGGAGCTAGCGAATTTT+TGG - chr8.1:33009930-33009949 MS.gene70003:intron 50.0%
!! GGTGCGGCAAACAGTTTTGT+TGG + chr8.1:33009811-33009830 None:intergenic 50.0%
ACCTTCCATTCGTCCTTCCC+TGG + chr8.1:33011086-33011105 None:intergenic 55.0%
AGAAGCCGCTTGACTGCAAC+TGG + chr8.1:33011120-33011139 None:intergenic 55.0%
ATTGGCCAGGGAAGGACGAA+TGG - chr8.1:33011078-33011097 MS.gene70003:intron 55.0%
CACATCAATTGCGTTCGGCC+TGG + chr8.1:33013139-33013158 None:intergenic 55.0%
CCAAAATCCTGATCCCGACG+TGG + chr8.1:33010475-33010494 None:intergenic 55.0%
GCTGTAGATTGGCGATCCTG+TGG + chr8.1:33009899-33009918 None:intergenic 55.0%
GGAGTTCATTCCGGAAGCTG+AGG - chr8.1:33012169-33012188 MS.gene70003:intron 55.0%
GGCTTCCCAACCTGTACAGT+AGG + chr8.1:33011991-33012010 None:intergenic 55.0%
GGTTCAAGTGGAGTCAGGGA+AGG - chr8.1:33011287-33011306 MS.gene70003:intron 55.0%
GTTAGAACGGACAGAGGAGG+AGG + chr8.1:33009625-33009644 None:intergenic 55.0%
TGTGGACACGCAACACGTTG+AGG - chr8.1:33013311-33013330 MS.gene70003:intron 55.0%
! CCACGTCGGGATCAGGATTT+TGG - chr8.1:33010472-33010491 MS.gene70003:intron 55.0%
ACAGAGGAGGAGGAGGAGCA+TGG + chr8.1:33009615-33009634 None:intergenic 60.0%
AGAACGGACAGAGGAGGAGG+AGG + chr8.1:33009622-33009641 None:intergenic 60.0%
GAAGCCCCAGAGCAGCATCA+AGG - chr8.1:33012005-33012024 MS.gene70003:CDS 60.0%
GCCAGGGAAGGACGAATGGA+AGG - chr8.1:33011082-33011101 MS.gene70003:intron 60.0%
GATGGTCCCACGTCGGGATC+AGG - chr8.1:33010465-33010484 MS.gene70003:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 33009547 33013506 33009547 ID=MS.gene70003
chr8.1 mRNA 33009547 33013506 33009547 ID=MS.gene70003.t1;Parent=MS.gene70003
chr8.1 exon 33013421 33013506 33013421 ID=MS.gene70003.t1.exon1;Parent=MS.gene70003.t1
chr8.1 CDS 33013421 33013506 33013421 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33013048 33013267 33013048 ID=MS.gene70003.t1.exon2;Parent=MS.gene70003.t1
chr8.1 CDS 33013048 33013267 33013048 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33012708 33012809 33012708 ID=MS.gene70003.t1.exon3;Parent=MS.gene70003.t1
chr8.1 CDS 33012708 33012809 33012708 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33012204 33012669 33012204 ID=MS.gene70003.t1.exon4;Parent=MS.gene70003.t1
chr8.1 CDS 33012204 33012669 33012204 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33011680 33012166 33011680 ID=MS.gene70003.t1.exon5;Parent=MS.gene70003.t1
chr8.1 CDS 33011680 33012166 33011680 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33011435 33011574 33011435 ID=MS.gene70003.t1.exon6;Parent=MS.gene70003.t1
chr8.1 CDS 33011435 33011574 33011435 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33010822 33011086 33010822 ID=MS.gene70003.t1.exon7;Parent=MS.gene70003.t1
chr8.1 CDS 33010822 33011086 33010822 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
chr8.1 exon 33009547 33009913 33009547 ID=MS.gene70003.t1.exon8;Parent=MS.gene70003.t1
chr8.1 CDS 33009547 33009913 33009547 ID=cds.MS.gene70003.t1;Parent=MS.gene70003.t1
Gene Sequence

>MS.gene70003

ATGAAAGTTCTCCCCTGGTTTCTGTTCCTCTTCCTCTTCTCGCTTTTTCAAGACTGTTGCTGCGACCATGCTCCTCCTCCTCCTCTGTCCGTTCTAACAACTCTTTTTCTTTCTTTTCTTTATCAATTATATATGCTAGGCTTGTTTGATTTGTACATGATATCTTATTATACAATTATTTTTTACTATTTTGATGTTGATATTCTCGTACTTATTTGTGCAAATGTTTTCATTGACAGTAATGAAGCAACAAAAATAATTCCAACAAAACTGTTTGCCGCACCTGATGGGACCATTTATTTTGTGGCCATCTACGAGAATGGTCCCATGAGGATATTATGGTCATTCTCCACAGGATCGCCAATCTACAGCTCGTATCAAGCTCCCGGAGCTAGCGAATTTTTGGACTGTGAGGATGATTGGTCATTGTACAAGGAGGATAAGTACTACGGCAAACTGGTATATGTTAGAACATTGTAATTGTAATTGGTATTTGAAATATGCGTTTTGCAGCATCAGCTTAACCTACATTAGCTAGAGAGTTAAACTTCACTACTGTGTCTGTAACTAACTAAATACAAAGAATATTTGAAGTAGAAAACTAATCTATTCTTACACTGTTTATTCAATTTTATTTTATTTTTTTTTTGAGGAAATTTGGGATTGATGTTCTGTTGGTTGTTTATCATGTCTGCAGATAATAGTAGAATCTATTAGAGAACTTGCACATCGTACGCCTATCATAATTTCATATAATGGAGAAGCTGTAATTGGATCTGAAAAAAGCACTTCGTTTCAAGTTGAAGCCAAAACTGGGTCGGTCAGTATAATAATAAGAATTTTGCTGGTCCTGGGAATCTAAATGCATCAAAGCCGCTGCTGATCAATATTTATAGGAAGGATTTCCATCTGAAGTATGATGGTCCCACGTCGGGATCAGGATTTTGGAATTTGACAGTAGCCGAGTTTGATTTTGTATTATTATGCCATCATCATACTACATTTCATGTAGAGGATCTCAATTTTACAATGCCTTACCCATGTCTAAAGAAGCAAAAAGTGTTCAAACTGACTAAGAACTTTCTGTTAGAATCTTTAATCAGTGAAAGTTCACATGGTGCTTATCATGGAAATGATACACTCTCAAATTCAATGCCTGCCTCAGATATGATGATTCAGTTGCAGCCTGATTTTGATAGTTTTTTCAATGATCATGATGGTAATATGGCAATGCCTCCTACGCCTTTTCTACAACAAAATAATTACAAGAGGAAAGATAAACTTCGTCAGCCTCTCACTGAGAGTGATTTACTAGGACATGTTTATTTGAACAAGAAGAGTGGGATGGCCAACTCCATCACCTAGTATGTTCGTTATTCTGTTGGTCGTCGTTTCACTCTACTGCTATCTCGTGGTAAAGGGGATTAAGTATAAGTATATACCAAAGGATAATAATATAGAAGTATCGATGAACTTTAATGAAGGTGTTGATGGAGAAATAATTGGCGAATTATTCGTTTCAAAAAAAGAGATTGGCCAGGGAAGGACGAATGGAAGGTATGATGGTCAACCAGTTGCAGTCAAGCGGCTTCTCAAATCTCATCATAGTCTAGCTCTAAACGAAATCAGAACACTTATGTCGGACCATCATCCGAACATTGTCAAATGGCATGGAGTGGAGTACGATGAAGATTTTATTTACCTTGCACTGGAGCGTTGTACTTGCAACTTAAATGATTTGGTTCAAGTGGAGTCAGGGAAGGATACTACAGAATATCTTTGGAAAAAAAATGACCATCCATCACCTCTACTACTTAAACTGATGAGGTTTGCATTATTTCTTCTTGAACCCTTACTTGTGCACATTGTAAGTGTAGTTTATTTTACACATTTTTAGTATAAACTAACTTCCATTTTTGTTTTGCAACTTAGGGGTATTGTTGCAGGGGTTGTTCATTTGCATAAACTAGGAATAATACACGGAAATTTGAAACCTCAAAATGTTCTAAAAATCAAGGATAGATCCTTATGTGTAAAGCTTTCTGACATGGGCATTAACAAACATGTTCCTGGTAAGCACTAAGATTTTTAAAAATTGTTTACATTTCCTAGCATTTAACTTGTGAATGTCCAATTATGTCTAGCATGGGATTTGCTTATGTAAATTACAATATGGTGATTTGTTAGATATCTCTTAATATCTTCAAAGTACTTTAATTGTCTCATAAGAGTTGTTTCCTAATTTTTGTTTTATTTTCAAAAAAATTCTATATCAATTAGCTCTTAGGAATTGTTTCCTAATTTTTGTTTTCTTTTCAAATTTGTTTCCATATCGATCGAATCTAATGTTATTCGGATTTGTTTCTCTTCTTTCTCTTTCCAAATTCATTGACCTTTTTCTCTTTATATTGTTTAAACAGGGAAGTCTGTTTTCGCCAAATCCTACTGTACAGGTTGGGAAGCCCCAGAGCAGCATCAAGGAATTGAAACACAAGCTACAGACATATTCAGTTTAGGTTGTATTTTTTTTTTCTGTTTGACTAAGGGAAGCCATCCATTTGGAGATGATCATCTTTGGCGTGAGTCCAATATCCTGAACGATCGAAAAGATCTTTCCTTAGTGGAGTTCATTCCGGAAGCTGAGGATCTTATTAGCTGTTTATTAAACCCTGACCAGAATCTAAGGTAAATCATTGTCATTCATTGTGGTTTTAGAGGTATATCTTCTCATACTTTCACAGTATTGCATCTGCTATGACTTGGTTAAATGTTATGTTGTATCAAATGATTAAGGCTCAAAGTTAATACCAACATACCTATTAATGTTATGAATAATAATTTAAGATGCAAGAGTGTTACCCTATTTTGATAACATTAATCTATTGTAGTTTTATTTGTTTTTATTGGTCTGCTGATTTCAGGAGGTCTTATTTTAAGCCAAGACTAAGATTTATTAGAATAGGTTAAATTTAAATTATGCTATTTCAATATAATTTCAGTCTCTAGCTTGCTATTGCGTATTTCTTTCTGGTTTGTTATTTTGCTTCTTCAGTTAATTATGTGTTGTTTTTTGCTGTTTGAGTCTCGTCTGGTTACCTTGGTTGTTATATCTTATCTTTTGAAGGGAATATTTATAAAAAGTTTAAAGTTCGGTAAGCTTTAGAGTTTAGATTGAAGGATAATGTTAGAATATAAAAAAAATGTTATAATATCTTCGATATTATGTCCTAGTATCCTAGTTTATCTCCCAAGATTAGTTTATAATCGTGTAGCGTATTTTAGACCCAAAAATTAGTTTTTATATTACATGGTTATTTGCCGATCTCACGTAGTAGGGTAAAGGTTTGATTGTTGTATTACACAGTTATTATCATGATTTGTTTCTTGATTTTCCTATTTATTTAGGAGTAGGAGCAGGAGCTTTGTATTTATATAAATAAGAGTTTGTGTTTAGTCTTTTGTACCAAATTGGTAGAAAGTAAATCAATCATATTACATTATTATCGTATTCTAAGTCTTTTATTATTTCTCTTCTTTTTTCTAAATCTCAATAACTTCAACAATCATAAAGAAATTATTTCCTTTATTTCCAGGCCGAACGCAATTGATGTGTTGCAGCATCCTTTTTTCCGAAATTCCCAGAAGAGAGTTTCATTTTTACTTGACACCAGTGACAAGACGGAACTAATTGATGACCCAATTGTGGTCAAGGAACATCAGATTGTTGAAAAGGAAAATATTAGGGTTGAGGAATCCAATGATGTTGTGGACACGCAACACGTTGAGGTTTCACCTGATCAGGTACAAGAGCAGGTTCTAAACTCTCCTTTAACTCATGATATGCAGAACAATGAGGCAGAAGTGCCTTTTACACCATACTTGACAAAGAAACAAAAGAAACAACTCAAAAGAGCATACAAAACTCATTCTAAGGGTGAACCATCTCCAACGCTGAAATGA
Protein sequence

>MS.gene70003.t1

MKVLPWFLFLFLFSLFQDCCCDHAPPPPLNEATKIIPTKLFAAPDGTIYFVAIYENGPMRILWSFSTGSPIYSSYQAPGASEFLDCEDDWSLYKEDKYYGKLIIVESIRELAHRTPIIISYNGEAVIGSEKSTSFQNFAGPGNLNASKPLLINIYRKDFHLKYDGPTSGSGFWNLTVAEFDFVLLCHHHTTFHVEDLNFTMPYPCLKKQKVFKLTKNFLLESLISESSHGAYHGNDTLSNSMPASDMMIQLQPDFDSFFNDHDGNMAMPPTPFLQQNNYKRKDKLRQPLTEMGWPTPSPSMFVILLVVVSLYCYLVVKGIKYKYIPKDNNIEVSMNFNEGVDGEIIGELFVSKKEIGQGRTNGRYDGQPVAVKRLLKSHHSLALNEIRTLMSDHHPNIVKWHGVEYDEDFIYLALERCTCNLNDLVQVESGKDTTEYLWKKNDHPSPLLLKLMRGIVAGVVHLHKLGIIHGNLKPQNVLKIKDRSLCVKLSDMGINKHVPGKSVFAKSYCTGWEAPEQHQGIETQATDIFSLGCIFFFCLTKGSHPFGDDHLWRESNILNDRKDLSLVEFIPEAEDLISCLLNPDQNLRPNAIDVLQHPFFRNSQKRVSFLLDTSDKTELIDDPIVVKEHQIVEKENIRVEESNDVVDTQHVEVSPDQVQEQVLNSPLTHDMQNNEAEVPFTPYLTKKQKKQLKRAYKTHSKGEPSPTLK