AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70195

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70195.t1 MTR_8g093920 98.734 79 1 0 1 79 54 132 6.40e-54 166
MS.gene70195.t1 MTR_5g095740 84.810 79 12 0 1 79 37 115 9.51e-48 150
MS.gene70195.t1 MTR_3g058980 83.544 79 13 0 1 79 41 119 1.72e-47 149
MS.gene70195.t1 MTR_2g026710 82.500 80 14 0 1 80 31 110 1.77e-46 146
MS.gene70195.t1 MTR_7g061270 79.747 79 16 0 1 79 40 118 3.76e-46 145
MS.gene70195.t1 MTR_7g100650 79.747 79 16 0 1 79 40 118 4.73e-46 145
MS.gene70195.t1 MTR_8g091720 81.013 79 15 0 1 79 42 120 3.02e-45 144
MS.gene70195.t1 MTR_1g072790 74.684 79 20 0 1 79 43 121 7.26e-44 139
MS.gene70195.t1 MTR_4g133938 77.215 79 18 0 1 79 33 111 2.23e-43 137
MS.gene70195.t1 MTR_1g088860 72.152 79 22 0 1 79 50 128 2.19e-40 131
MS.gene70195.t1 MTR_0392s0020 66.667 78 26 0 1 78 32 109 1.55e-38 125
MS.gene70195.t1 MTR_4g119500 66.667 78 26 0 1 78 36 113 4.42e-38 123
MS.gene70195.t1 MTR_4g133952 62.025 79 30 0 1 79 72 150 4.40e-37 124
MS.gene70195.t1 MTR_1g039040 62.025 79 30 0 1 79 20 98 4.36e-36 120
MS.gene70195.t1 MTR_1g083070 60.256 78 31 0 1 78 18 95 5.39e-32 108
MS.gene70195.t1 MTR_1g029070 51.852 81 39 0 1 81 66 146 1.60e-25 93.2
MS.gene70195.t1 MTR_1g028480 51.562 64 31 0 17 80 19 82 5.04e-19 74.7
MS.gene70195.t1 MTR_5g095900 40.000 75 45 0 1 75 30 104 1.18e-14 65.5
MS.gene70195.t1 MTR_1g029100 38.806 67 41 0 1 67 16 82 1.25e-14 63.2
MS.gene70195.t1 MTR_2g056000 29.333 75 53 0 1 75 55 129 4.91e-12 58.5
MS.gene70195.t1 MTR_4g112380 26.667 75 55 0 1 75 25 99 4.54e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70195.t1 AT4G14540 87.342 79 10 0 1 79 35 113 2.38e-49 153
MS.gene70195.t1 AT2G13570 92.405 79 6 0 1 79 50 128 1.62e-48 152
MS.gene70195.t1 AT5G47640 83.750 80 13 0 1 80 41 120 2.56e-47 149
MS.gene70195.t1 AT2G37060 79.747 79 16 0 1 79 44 122 5.06e-45 142
MS.gene70195.t1 AT2G37060 79.747 79 16 0 1 79 44 122 5.06e-45 142
MS.gene70195.t1 AT2G37060 79.747 79 16 0 1 79 44 122 5.06e-45 142
MS.gene70195.t1 AT3G53340 77.215 79 18 0 1 79 43 121 2.29e-44 141
MS.gene70195.t1 AT3G53340 77.215 79 18 0 1 79 43 121 2.29e-44 141
MS.gene70195.t1 AT2G38880 75.949 79 19 0 1 79 35 113 4.15e-44 139
MS.gene70195.t1 AT2G38880 75.949 79 19 0 1 79 35 113 6.27e-44 139
MS.gene70195.t1 AT2G38880 75.949 79 19 0 1 79 35 113 6.27e-44 139
MS.gene70195.t1 AT2G38880 75.949 79 19 0 1 79 35 113 6.27e-44 139
MS.gene70195.t1 AT3G53340 76.623 77 18 0 1 77 43 119 6.26e-43 135
MS.gene70195.t1 AT3G53340 76.923 78 18 0 1 78 43 120 1.65e-42 135
MS.gene70195.t1 AT3G53340 76.923 78 18 0 1 78 43 120 1.65e-42 135
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 2.38e-42 134
MS.gene70195.t1 AT2G38880 76.316 76 18 0 1 76 35 110 5.32e-42 134
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 6.13e-42 134
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 6.13e-42 134
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 8.82e-42 134
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 8.82e-42 134
MS.gene70195.t1 AT2G38880 75.325 77 19 0 1 77 35 111 8.82e-42 134
MS.gene70195.t1 AT2G47810 70.886 79 23 0 1 79 65 143 3.63e-39 127
MS.gene70195.t1 AT5G47670 60.759 79 31 0 1 79 43 121 4.61e-37 123
MS.gene70195.t1 AT5G47670 60.759 79 31 0 1 79 72 150 7.36e-37 124
MS.gene70195.t1 AT5G47670 60.759 79 31 0 1 79 72 150 7.36e-37 124
MS.gene70195.t1 AT1G21970 61.538 78 30 0 1 78 73 150 1.74e-34 117
MS.gene70195.t1 AT1G09030 61.538 78 30 0 1 78 17 94 2.41e-33 112
MS.gene70195.t1 AT5G08190 32.530 83 50 1 1 83 25 101 3.88e-13 61.2
MS.gene70195.t1 AT5G08190 32.530 83 50 1 1 83 25 101 4.07e-13 60.8
MS.gene70195.t1 AT5G23090 32.530 83 50 1 1 83 25 101 7.74e-13 60.1
MS.gene70195.t1 AT5G23090 32.530 83 50 1 1 83 25 101 7.88e-13 60.1
MS.gene70195.t1 AT5G23090 32.530 83 50 1 1 83 25 101 7.88e-13 60.1
MS.gene70195.t1 AT5G23090 32.530 83 50 1 1 83 25 101 7.88e-13 60.1
MS.gene70195.t1 AT5G23090 32.911 79 47 1 5 83 16 88 1.14e-11 57.0

Find 7 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGGTGATGATATTATT+TGG 0.131471 4.1:+8128664 MS.gene70195:CDS
CAATGGTGATGATATTATTT+GGG 0.366036 4.1:+8128665 MS.gene70195:CDS
GTTAGGCTTTGAAGATTATG+TGG 0.475398 4.1:+8128698 MS.gene70195:CDS
ACATTTATCCGATGCTTCCC+CGG 0.490805 4.1:-8128619 None:intergenic
ATTTGGGCTATTACAACGTT+AGG 0.507634 4.1:+8128681 MS.gene70195:CDS
AGATTGATTCCATAGGGCAT+TGG 0.544180 4.1:+8128988 MS.gene70195:intron
GACAGTTCAAGAATGTGTGT+CGG 0.617021 4.1:+8128578 MS.gene70195:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATATAGAGATATTGA+AGG + chr4.1:8128741-8128760 MS.gene70195:CDS 20.0%
!!! AATTGTTTTTGAATCGAATT+TGG - chr4.1:8128954-8128973 None:intergenic 20.0%
!!! CAATATCTCTATATTTTTGA+AGG - chr4.1:8128740-8128759 None:intergenic 20.0%
!!! TTTTGAAGGTAACATTTTAA+TGG - chr4.1:8128726-8128745 None:intergenic 20.0%
! AAAAAAGTTATTCCTGCAAA+TGG + chr4.1:8128531-8128550 MS.gene70195:CDS 25.0%
! GAAAAAAGAAAGACAATCAA+TGG + chr4.1:8128648-8128667 MS.gene70195:CDS 25.0%
!! CAATGGTGATGATATTATTT+GGG + chr4.1:8128665-8128684 MS.gene70195:CDS 25.0%
!! TCAATGGTGATGATATTATT+TGG + chr4.1:8128664-8128683 MS.gene70195:CDS 25.0%
AACAACAAAGATCTGAACAA+AGG + chr4.1:8128784-8128803 MS.gene70195:intron 30.0%
TGCAAATGGAAAAATCTCAA+AGG + chr4.1:8128545-8128564 MS.gene70195:CDS 30.0%
! AAGATCAGATTGATTCCATA+GGG + chr4.1:8128982-8129001 MS.gene70195:intron 30.0%
!! TTTGAGATTTTTCCATTTGC+AGG - chr4.1:8128546-8128565 None:intergenic 30.0%
!!! TGTTCAGATCTTTGTTGTTT+TGG - chr4.1:8128783-8128802 None:intergenic 30.0%
ATGGAATCAATCTGATCTTG+CGG - chr4.1:8128981-8129000 None:intergenic 35.0%
ATTTGGGCTATTACAACGTT+AGG + chr4.1:8128681-8128700 MS.gene70195:CDS 35.0%
GGTTGATCAGTAGTCATATA+AGG - chr4.1:8128912-8128931 None:intergenic 35.0%
TGAATCGAATTTGGAGAGAA+AGG - chr4.1:8128945-8128964 None:intergenic 35.0%
TGATCAGTAGTCATATAAGG+AGG - chr4.1:8128909-8128928 None:intergenic 35.0%
! CAAGATCAGATTGATTCCAT+AGG + chr4.1:8128981-8129000 MS.gene70195:intron 35.0%
!! AGTTCATAAGTTTTGTCACC+GGG + chr4.1:8128601-8128620 MS.gene70195:CDS 35.0%
!! GAGTTCATAAGTTTTGTCAC+CGG + chr4.1:8128600-8128619 MS.gene70195:CDS 35.0%
!! GTTAGGCTTTGAAGATTATG+TGG + chr4.1:8128698-8128717 MS.gene70195:CDS 35.0%
GAATTTGGAGAGAAAGGTAG+TGG - chr4.1:8128939-8128958 None:intergenic 40.0%
GACAGTTCAAGAATGTGTGT+CGG + chr4.1:8128578-8128597 MS.gene70195:CDS 40.0%
TCATTCATACCAATGCCCTA+TGG - chr4.1:8129000-8129019 None:intergenic 40.0%
! AGATTGATTCCATAGGGCAT+TGG + chr4.1:8128988-8129007 MS.gene70195:intron 40.0%
!! GTTCATAAGTTTTGTCACCG+GGG + chr4.1:8128602-8128621 MS.gene70195:CDS 40.0%
ACATTTATCCGATGCTTCCC+CGG - chr4.1:8128622-8128641 None:intergenic 45.0%
!! GGAGAGAAAGGTAGTGGAAA+TGG - chr4.1:8128933-8128952 None:intergenic 45.0%
!! TTTTGTCACCGGGGAAGCAT+CGG + chr4.1:8128611-8128630 MS.gene70195:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8128528 8129019 8128528 ID=MS.gene70195
chr4.1 mRNA 8128528 8129019 8128528 ID=MS.gene70195.t1;Parent=MS.gene70195
chr4.1 exon 8128528 8128762 8128528 ID=MS.gene70195.t1.exon1;Parent=MS.gene70195.t1
chr4.1 CDS 8128528 8128762 8128528 ID=cds.MS.gene70195.t1;Parent=MS.gene70195.t1
chr4.1 exon 8129003 8129019 8129003 ID=MS.gene70195.t1.exon2;Parent=MS.gene70195.t1
chr4.1 CDS 8129003 8129019 8129003 ID=cds.MS.gene70195.t1;Parent=MS.gene70195.t1
Gene Sequence

>MS.gene70195

ATGAAAAAAGTTATTCCTGCAAATGGAAAAATCTCAAAGGATGCAAAAGAGACAGTTCAAGAATGTGTGTCGGAGTTCATAAGTTTTGTCACCGGGGAAGCATCGGATAAATGTCAAAGAGAAAAAAGAAAGACAATCAATGGTGATGATATTATTTGGGCTATTACAACGTTAGGCTTTGAAGATTATGTGGAACCATTAAAATGTTACCTTCAAAAATATAGAGATATTGAAGGTGAAAAAGTCAATGTTCCAAAACAACAAAGATCTGAACAAAGGCTACATCAACATCAACATCAACATCAACATAATCAAGATGAATTAAATAATCAACATTTCAACAATAGTGTATATACATCTACAAATCTCATGTCTCAACCTCCTTATATGACTACTGATCAACCATTTCCACTACCTTTCTCTCCAAATTCGATTCAAAAACAATTACGACCGCAAGATCAGATTGATTCCATAGGGCATTGGTATGAATGA
Protein sequence

>MS.gene70195.t1

MKKVIPANGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGDDIIWAITTLGFEDYVEPLKCYLQKYRDIEGHWYE