AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70243

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70243.t1 MTR_8g093070 95.858 507 21 0 1 507 1 507 0.0 1005
MS.gene70243.t1 MTR_8g092440 86.364 154 19 1 1 154 1 152 4.78e-87 265
MS.gene70243.t1 MTR_3g072710 38.095 399 194 12 136 496 117 500 7.92e-66 221
MS.gene70243.t1 MTR_5g058860 38.060 402 210 14 125 496 105 497 9.94e-65 218
MS.gene70243.t1 MTR_3g065980 33.075 387 216 14 136 495 169 539 8.95e-40 151
MS.gene70243.t1 MTR_7g027190 30.958 407 223 15 126 495 287 672 7.43e-39 150
MS.gene70243.t1 MTR_4g076020 31.081 370 215 11 144 499 89 432 7.49e-37 141
MS.gene70243.t1 MTR_4g102790 29.177 401 233 16 136 504 289 670 2.62e-36 143
MS.gene70243.t1 MTR_7g074650 27.614 373 229 14 144 497 443 793 4.56e-33 134
MS.gene70243.t1 MTR_4g077760 31.818 374 216 17 144 495 198 554 5.09e-31 126
MS.gene70243.t1 MTR_1g096030 30.633 395 226 18 136 495 77 458 3.92e-27 114
MS.gene70243.t1 MTR_1g069725 26.042 384 237 13 135 496 105 463 7.67e-26 110
MS.gene70243.t1 MTR_2g082090 27.604 384 240 16 136 495 209 578 1.03e-25 111
MS.gene70243.t1 MTR_4g133660 25.189 397 261 14 123 496 160 543 2.27e-25 109
MS.gene70243.t1 MTR_8g442410 26.823 384 246 13 136 495 152 524 3.28e-25 109
MS.gene70243.t1 MTR_0092s0100 26.542 373 253 9 133 495 369 730 4.47e-25 109
MS.gene70243.t1 MTR_0092s0100 26.542 373 253 9 133 495 366 727 4.83e-25 109
MS.gene70243.t1 MTR_5g094450 25.542 415 268 15 111 495 128 531 7.49e-25 108
MS.gene70243.t1 MTR_2g034260 26.250 400 252 14 126 495 197 583 7.95e-25 108
MS.gene70243.t1 MTR_4g064150 25.862 406 248 17 127 498 349 735 2.29e-24 107
MS.gene70243.t1 MTR_2g034250 27.160 405 243 16 126 495 197 584 3.82e-24 106
MS.gene70243.t1 MTR_5g097480 27.518 407 253 18 114 495 154 543 4.62e-24 105
MS.gene70243.t1 MTR_3g056110 27.157 394 254 16 123 495 160 541 4.80e-24 105
MS.gene70243.t1 MTR_5g015490 26.684 386 250 16 138 497 113 491 6.36e-24 105
MS.gene70243.t1 MTR_3g089055 26.649 379 244 12 139 495 198 564 1.52e-23 104
MS.gene70243.t1 MTR_3g089055 26.649 379 244 12 139 495 203 569 1.52e-23 104
MS.gene70243.t1 MTR_1g029420 27.368 380 240 14 139 495 225 591 3.01e-23 103
MS.gene70243.t1 MTR_7g062120 25.485 412 259 17 115 498 351 742 3.34e-23 103
MS.gene70243.t1 MTR_2g097410 24.623 398 261 16 127 498 359 743 5.78e-23 103
MS.gene70243.t1 MTR_2g097473 24.474 380 245 16 144 498 294 656 8.80e-23 102
MS.gene70243.t1 MTR_4g064200 25.648 386 244 15 139 498 284 652 1.13e-22 102
MS.gene70243.t1 MTR_2g034280 26.980 404 242 17 126 495 190 574 1.24e-22 101
MS.gene70243.t1 MTR_2g097467 24.403 377 249 16 144 498 294 656 1.58e-22 101
MS.gene70243.t1 MTR_2g026250 26.257 358 220 15 168 495 252 595 1.89e-22 101
MS.gene70243.t1 MTR_4g097080 26.166 386 246 14 138 497 132 504 4.10e-22 99.8
MS.gene70243.t1 MTR_2g097463 24.359 390 256 17 134 498 282 657 1.29e-21 99.0
MS.gene70243.t1 MTR_4g104020 27.692 390 236 16 136 495 146 519 1.94e-21 97.8
MS.gene70243.t1 MTR_4g064120 24.495 396 248 16 136 498 251 628 5.94e-20 93.6
MS.gene70243.t1 MTR_4g026485 26.702 382 240 16 133 495 263 623 1.82e-18 89.0
MS.gene70243.t1 MTR_4g064180 25.231 325 200 13 136 434 251 558 5.44e-18 87.4
MS.gene70243.t1 MTR_2g097350 23.426 397 273 13 122 498 257 642 8.43e-18 87.0
MS.gene70243.t1 MTR_8g077940 27.005 374 247 13 136 495 179 540 9.45e-18 86.7
MS.gene70243.t1 MTR_4g064160 25.570 395 241 18 136 498 313 686 1.43e-17 86.3
MS.gene70243.t1 MTR_2g097310 23.969 388 262 15 132 498 265 640 2.73e-17 85.5
MS.gene70243.t1 MTR_2g097390 22.727 396 277 14 122 498 258 643 1.19e-16 83.6
MS.gene70243.t1 MTR_7g109580 28.523 298 174 14 219 495 274 553 1.82e-15 79.3
MS.gene70243.t1 MTR_5g015950 22.248 427 252 12 139 498 35 448 4.15e-15 77.8
MS.gene70243.t1 MTR_3g053270 22.248 427 252 12 139 498 35 448 4.15e-15 77.8
MS.gene70243.t1 MTR_2g099110 24.444 360 216 14 177 497 110 452 2.18e-14 75.9
MS.gene70243.t1 MTR_1g086970 25.062 403 251 17 126 495 93 477 2.57e-14 75.5
MS.gene70243.t1 MTR_5g009080 29.834 181 116 7 136 310 53 228 1.80e-13 72.8
MS.gene70243.t1 MTR_4g095500 22.565 421 254 14 139 497 59 469 4.38e-13 71.6
MS.gene70243.t1 MTR_2g097380 22.930 314 198 10 203 498 276 563 2.19e-12 69.7
MS.gene70243.t1 MTR_4g076140 28.155 206 134 7 136 332 45 245 2.68e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70243.t1 AT4G08250 32.905 389 228 14 136 501 104 482 1.07e-47 172
MS.gene70243.t1 AT1G55580 35.238 315 162 12 209 495 144 444 4.03e-42 156
MS.gene70243.t1 AT5G41920 32.275 378 220 14 136 495 40 399 1.44e-40 151
MS.gene70243.t1 AT3G03450 29.973 377 235 13 136 495 180 544 5.11e-37 144
MS.gene70243.t1 AT1G66350 29.178 377 227 14 136 495 152 505 2.02e-35 139
MS.gene70243.t1 AT5G17490 30.311 386 230 14 130 495 149 515 8.39e-35 137
MS.gene70243.t1 AT2G01570 29.351 385 222 16 136 495 221 580 1.06e-32 132
MS.gene70243.t1 AT1G14920 28.424 387 223 16 136 495 169 528 1.14e-31 128
MS.gene70243.t1 AT3G54220 27.394 376 229 14 144 497 298 651 5.82e-31 127
MS.gene70243.t1 AT1G63100 25.000 404 251 14 129 496 242 629 4.24e-30 124
MS.gene70243.t1 AT5G66770 29.738 343 203 12 177 495 255 583 4.46e-30 124
MS.gene70243.t1 AT1G63100 25.000 404 251 14 129 496 267 654 7.22e-30 124
MS.gene70243.t1 AT5G59450 27.475 404 241 18 136 506 224 608 8.41e-30 123
MS.gene70243.t1 AT3G50650 29.661 354 211 14 167 495 201 541 8.52e-29 120
MS.gene70243.t1 AT4G17230 29.073 399 238 16 128 497 144 526 1.20e-28 119
MS.gene70243.t1 AT4G17230 29.073 399 238 16 128 497 144 526 1.20e-28 119
MS.gene70243.t1 AT2G04890 28.465 404 244 18 119 495 27 412 9.88e-28 115
MS.gene70243.t1 AT3G46600 28.278 389 233 15 138 498 3 373 7.92e-27 112
MS.gene70243.t1 AT1G50600 26.166 386 257 13 129 495 149 525 8.22e-27 114
MS.gene70243.t1 AT3G46600 27.423 423 256 16 103 497 181 580 8.46e-27 114
MS.gene70243.t1 AT3G46600 27.423 423 256 16 103 497 149 548 8.65e-27 114
MS.gene70243.t1 AT1G50600 26.166 386 257 13 129 495 224 600 1.72e-26 113
MS.gene70243.t1 AT1G50600 26.166 386 257 13 129 495 220 596 1.89e-26 113
MS.gene70243.t1 AT5G48150 27.833 406 247 16 118 495 102 489 2.08e-26 112
MS.gene70243.t1 AT5G48150 27.833 406 247 16 118 495 102 489 2.08e-26 112
MS.gene70243.t1 AT5G48150 27.833 406 247 16 118 495 102 489 2.08e-26 112
MS.gene70243.t1 AT5G48150 27.833 406 247 16 118 495 102 489 2.08e-26 112
MS.gene70243.t1 AT1G07530 26.698 427 253 18 104 498 369 767 3.02e-25 110
MS.gene70243.t1 AT1G21450 27.356 435 270 18 87 495 178 592 1.47e-24 107
MS.gene70243.t1 AT4G37650 26.531 392 250 14 139 498 146 531 3.72e-24 106
MS.gene70243.t1 AT2G29060 26.617 402 237 20 132 497 277 656 8.91e-23 102
MS.gene70243.t1 AT2G29060 26.617 402 237 20 132 497 314 693 8.92e-23 102
MS.gene70243.t1 AT2G37650 24.490 392 263 12 127 497 323 702 2.18e-21 98.2
MS.gene70243.t1 AT2G37650 24.490 392 263 12 127 497 335 714 2.53e-21 98.2
MS.gene70243.t1 AT4G00150 27.851 377 229 17 135 495 204 553 2.40e-20 94.7
MS.gene70243.t1 AT1G07520 23.907 389 256 14 139 498 318 695 4.29e-18 88.2
MS.gene70243.t1 AT1G07520 23.907 389 256 14 139 498 319 696 4.41e-18 88.2
MS.gene70243.t1 AT1G07520 23.907 389 256 14 139 498 154 531 7.41e-18 87.0
MS.gene70243.t1 AT1G07520 23.907 389 256 14 139 498 154 531 7.41e-18 87.0
MS.gene70243.t1 AT4G36710 27.100 369 249 14 138 495 125 484 1.07e-17 86.3
MS.gene70243.t1 AT5G52510 26.667 315 191 11 212 495 334 639 3.04e-17 85.5
MS.gene70243.t1 AT2G29065 24.768 323 201 12 203 498 323 630 3.70e-17 85.1
MS.gene70243.t1 AT1G50420 23.733 434 248 16 136 495 54 478 4.02e-17 84.3
MS.gene70243.t1 AT2G45160 24.365 394 226 17 141 495 278 638 7.83e-16 80.9
MS.gene70243.t1 AT3G49950 24.103 390 255 13 139 497 30 409 1.81e-15 79.0
MS.gene70243.t1 AT3G60630 24.451 319 184 12 209 495 328 621 1.59e-13 73.6

Find 77 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCTCTTTCTCTATA+AGG 0.158083 4.1:+8690200 None:intergenic
ATTTCATCTAGGATTGTATT+TGG 0.213674 4.1:+8691306 None:intergenic
ATTTCGTCTAGGATTGTATT+TGG 0.226044 4.1:+8691246 None:intergenic
CTAAACAATGCCTCGTCTTT+AGG 0.252519 4.1:-8690940 MS.gene70243:CDS
GGAAGTTCAAGAAGAGTTAA+TGG 0.258644 4.1:-8691052 MS.gene70243:CDS
CTGTGATTGCTGTTACTCTA+AGG 0.272598 4.1:+8690614 None:intergenic
GCTTGTTTGGTAAAGGGTAT+TGG 0.274576 4.1:-8690030 MS.gene70243:CDS
AAGCTATTGGTTAGCTTGTT+TGG 0.278423 4.1:-8690043 MS.gene70243:CDS
AACAAGCCTTGGTGATGATT+TGG 0.290948 4.1:+8690369 None:intergenic
CTTTGACTTCTGTTTCAATC+TGG 0.297827 4.1:-8689961 MS.gene70243:CDS
CTTGTTTGGTAAAGGGTATT+GGG 0.313644 4.1:-8690029 MS.gene70243:CDS
CCAGGGAAGAAGATTCTTAT+TGG 0.318155 4.1:-8691077 MS.gene70243:CDS
GCGAAAATGTGTTTGTTTCC+AGG 0.325069 4.1:-8691095 MS.gene70243:CDS
CAATACCTCTTTCTCTATAA+GGG 0.331359 4.1:+8690201 None:intergenic
TGTGGTGGAGTTACCGAAAA+AGG 0.334912 4.1:-8691120 MS.gene70243:CDS
TATTTCACAGGCTAAGCTAT+TGG 0.357597 4.1:-8690056 MS.gene70243:CDS
CTCATAATCCATCACATTCA+TGG 0.366173 4.1:+8691425 None:intergenic
TGGTTAGCTTGTTTGGTAAA+GGG 0.385802 4.1:-8690036 MS.gene70243:CDS
TAAGGGCTAACTCCATCTTA+TGG 0.393772 4.1:+8690218 None:intergenic
AAGGCGATAGTTTATTGAAC+AGG 0.393824 4.1:-8690915 MS.gene70243:CDS
CCTTATAGAGAAAGAGGTAT+TGG 0.394224 4.1:-8690200 MS.gene70243:CDS
GATCAAACAATATTTCATCT+AGG 0.398157 4.1:+8691295 None:intergenic
AATCCAATGGCCACCGTTGA+TGG 0.402840 4.1:-8690668 MS.gene70243:CDS
TTGGTTAGCTTGTTTGGTAA+AGG 0.407587 4.1:-8690037 MS.gene70243:CDS
ATCAATGAGAAACTCCTGTT+TGG 0.411022 4.1:+8690415 None:intergenic
ACTCAAATCCATGTCCTTTG+TGG 0.411956 4.1:-8690311 MS.gene70243:CDS
TTTGACTTCTGTTTCAATCT+GGG 0.412837 4.1:-8689960 MS.gene70243:CDS
TACATCAGTGGATGCCAAAC+AGG 0.416877 4.1:-8690429 MS.gene70243:CDS
AGCTTCGAAAATCGCTTGAT+TGG 0.418613 4.1:+8690741 None:intergenic
CTAAGCTACATTCATGCAGC+TGG 0.426844 4.1:+8691367 None:intergenic
CTTATAGAGAAAGAGGTATT+GGG 0.435957 4.1:-8690199 MS.gene70243:CDS
GAGATAAGAGATGTTGTGAT+TGG 0.464477 4.1:+8691268 None:intergenic
CAACCATCAACGGTGGCCAT+TGG 0.475147 4.1:+8690665 None:intergenic
CCAATAAGAATCTTCTTCCC+TGG 0.475253 4.1:+8691077 None:intergenic
GAGACATGAGATCTTCTGCT+TGG 0.478088 4.1:+8691328 None:intergenic
CGAAAATGTGTTTGTTTCCA+GGG 0.485498 4.1:-8691094 MS.gene70243:CDS
GTTAGCCCTTATAGAGAAAG+AGG 0.487244 4.1:-8690206 MS.gene70243:CDS
AATGCCTCGTCTTTAGGAAA+AGG 0.493064 4.1:-8690934 MS.gene70243:CDS
TCGACTAAAACAACAAGCCT+TGG 0.505560 4.1:+8690358 None:intergenic
ACATGTTCTGAATGGAACTT+AGG 0.509744 4.1:+8690844 None:intergenic
GCAATATGAGAACTGTAAGT+TGG 0.510443 4.1:-8690005 MS.gene70243:CDS
AAAAGAGGAGAGAATATTGT+GGG 0.510893 4.1:-8690137 MS.gene70243:CDS
GAAGCCTCAGTTCAACAAAC+AGG 0.521298 4.1:-8690577 MS.gene70243:CDS
TTACTCATATTATAATCTGT+TGG 0.522232 4.1:+8689935 None:intergenic
ACTGTAAGTTGGCTTTGTGT+TGG 0.525441 4.1:-8689994 MS.gene70243:CDS
GAATTCAAAGAAATTGAACT+TGG 0.527275 4.1:-8690478 MS.gene70243:CDS
CACGTTCGATACAGTAATGA+TGG 0.531854 4.1:-8690512 MS.gene70243:CDS
GGAAGAAGATTCTTATTGGA+AGG 0.535054 4.1:-8691073 MS.gene70243:CDS
GTGTAGAAACATGTTCTGAA+TGG 0.541579 4.1:+8690836 None:intergenic
CTTGATCGAAGATTTCGTCT+AGG 0.546863 4.1:+8691235 None:intergenic
GCAGCTGGTTCATGCAAGGA+GGG 0.553162 4.1:+8691382 None:intergenic
AGATCTTCTGCTTGGAGTGG+TGG 0.557382 4.1:+8691336 None:intergenic
TTGCTGCAGCAATTCTTGCA+TGG 0.560772 4.1:+8691206 None:intergenic
GATTCATGATATTGAAGTCG+AGG 0.561299 4.1:+8691175 None:intergenic
CATGAATCATGAACAAGCAA+TGG 0.561832 4.1:-8691160 MS.gene70243:CDS
TCTGTGGAGTAGCCATAAGA+TGG 0.563300 4.1:-8690230 MS.gene70243:CDS
TTGAAGGGGTATAGACGTGT+TGG 0.563890 4.1:-8690094 MS.gene70243:CDS
ACTATCTACCATGAATGTGA+TGG 0.583158 4.1:-8691433 None:intergenic
TCTGCCTGTTTGTTGAACTG+AGG 0.584240 4.1:+8690573 None:intergenic
TGTGATTGCTGTTACTCTAA+GGG 0.585072 4.1:+8690615 None:intergenic
GAGTACATGCAATATTTCAC+AGG 0.587078 4.1:-8690068 MS.gene70243:CDS
ATTACTGTATCGAACGTGAA+CGG 0.593883 4.1:+8690517 None:intergenic
CAGTTCAACAAACAGGCAGA+AGG 0.599250 4.1:-8690570 MS.gene70243:CDS
TCATGCAGCTGGTTCATGCA+AGG 0.599943 4.1:+8691378 None:intergenic
TGCAGCTGGTTCATGCAAGG+AGG 0.602202 4.1:+8691381 None:intergenic
ATTGCAAAGTCAACCATCAA+CGG 0.607337 4.1:+8690655 None:intergenic
CAGATTGAAACAGAAGTCAA+AGG 0.620209 4.1:+8689962 None:intergenic
ATGAGATCTTCTGCTTGGAG+TGG 0.651380 4.1:+8691333 None:intergenic
ATATCATGGAGGGAATCCAA+TGG 0.669550 4.1:-8690681 MS.gene70243:CDS
CTAGAGAATGAATTGTGTGG+TGG 0.675354 4.1:-8691135 MS.gene70243:CDS
CAGCTGGTTCATGCAAGGAG+GGG 0.677543 4.1:+8691383 None:intergenic
ATTCACTTGTAAGTACTCTG+TGG 0.679195 4.1:-8690246 MS.gene70243:CDS
TCAATTGTATGATACATCAG+TGG 0.691913 4.1:-8690441 MS.gene70243:CDS
GCAAAGTCAACCATCAACGG+TGG 0.711871 4.1:+8690658 None:intergenic
ACGTAACCAAATCATCACCA+AGG 0.735440 4.1:-8690375 MS.gene70243:CDS
TAGAGTAACAGCAATCACAG+TGG 0.741841 4.1:-8690611 MS.gene70243:CDS
GCTCTAGAGAATGAATTGTG+TGG 0.769887 4.1:-8691138 MS.gene70243:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTTTATTCATTGA+AGG - chr4.1:8691235-8691254 MS.gene70243:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG - chr4.1:8691236-8691255 MS.gene70243:CDS 15.0%
!! TTACTCATATTATAATCTGT+TGG + chr4.1:8691413-8691432 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG + chr4.1:8690580-8690599 None:intergenic 20.0%
!!! AAATGAGCAAATTTTACATA+GGG + chr4.1:8690579-8690598 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG - chr4.1:8691237-8691256 MS.gene70243:CDS 20.0%
! GAATTCAAAGAAATTGAACT+TGG - chr4.1:8690867-8690886 MS.gene70243:CDS 25.0%
! GATCAAACAATATTTCATCT+AGG + chr4.1:8690053-8690072 None:intergenic 25.0%
!! ATTTCATCTAGGATTGTATT+TGG + chr4.1:8690042-8690061 None:intergenic 25.0%
!!! ATTGATTTTGATATCATGGA+GGG - chr4.1:8690654-8690673 MS.gene70243:CDS 25.0%
!!! TGTCATTGATTTTGATATCA+TGG - chr4.1:8690650-8690669 MS.gene70243:CDS 25.0%
AAAAAGAGGAGAGAATATTG+TGG - chr4.1:8691207-8691226 MS.gene70243:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG - chr4.1:8691208-8691227 MS.gene70243:CDS 30.0%
CAATACCTCTTTCTCTATAA+GGG + chr4.1:8691147-8691166 None:intergenic 30.0%
TCAATTGTATGATACATCAG+TGG - chr4.1:8690904-8690923 MS.gene70243:CDS 30.0%
! ATTTCGTCTAGGATTGTATT+TGG + chr4.1:8690102-8690121 None:intergenic 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG - chr4.1:8690327-8690346 MS.gene70243:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG - chr4.1:8691385-8691404 MS.gene70243:CDS 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG - chr4.1:8691146-8691165 MS.gene70243:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG - chr4.1:8690653-8690672 MS.gene70243:CDS 30.0%
AAGGCGATAGTTTATTGAAC+AGG - chr4.1:8690430-8690449 MS.gene70243:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG - chr4.1:8691193-8691212 MS.gene70243:CDS 35.0%
ATCAATGAGAAACTCCTGTT+TGG + chr4.1:8690933-8690952 None:intergenic 35.0%
ATTACTGTATCGAACGTGAA+CGG + chr4.1:8690831-8690850 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG - chr4.1:8691099-8691118 MS.gene70243:CDS 35.0%
ATTGCAAAGTCAACCATCAA+CGG + chr4.1:8690693-8690712 None:intergenic 35.0%
CAGATTGAAACAGAAGTCAA+AGG + chr4.1:8691386-8691405 None:intergenic 35.0%
CATGAATCATGAACAAGCAA+TGG - chr4.1:8690185-8690204 MS.gene70243:CDS 35.0%
CCAATACCTCTTTCTCTATA+AGG + chr4.1:8691148-8691167 None:intergenic 35.0%
CGAAAATGTGTTTGTTTCCA+GGG - chr4.1:8690251-8690270 MS.gene70243:CDS 35.0%
GAGATAAGAGATGTTGTGAT+TGG + chr4.1:8690080-8690099 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG - chr4.1:8691277-8691296 MS.gene70243:CDS 35.0%
GATTCATGATATTGAAGTCG+AGG + chr4.1:8690173-8690192 None:intergenic 35.0%
GCAATATGAGAACTGTAAGT+TGG - chr4.1:8691340-8691359 MS.gene70243:CDS 35.0%
GGAAGAAGATTCTTATTGGA+AGG - chr4.1:8690272-8690291 MS.gene70243:CDS 35.0%
GGAAGTTCAAGAAGAGTTAA+TGG - chr4.1:8690293-8690312 MS.gene70243:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG - chr4.1:8691289-8691308 MS.gene70243:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG + chr4.1:8690504-8690523 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG - chr4.1:8691145-8691164 MS.gene70243:CDS 35.0%
! CTTTGACTTCTGTTTCAATC+TGG - chr4.1:8691384-8691403 MS.gene70243:CDS 35.0%
! GTGTAGAAACATGTTCTGAA+TGG + chr4.1:8690512-8690531 None:intergenic 35.0%
! TGGAAACAAACACATTTTCG+CGG + chr4.1:8690251-8690270 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG + chr4.1:8690733-8690752 None:intergenic 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG - chr4.1:8690453-8690472 MS.gene70243:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG - chr4.1:8691302-8691321 MS.gene70243:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG + chr4.1:8691203-8691222 None:intergenic 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG - chr4.1:8691316-8691335 MS.gene70243:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG - chr4.1:8691309-8691328 MS.gene70243:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG - chr4.1:8691308-8691327 MS.gene70243:CDS 35.0%
ACGTAACCAAATCATCACCA+AGG - chr4.1:8690970-8690989 MS.gene70243:CDS 40.0%
ACTCAAATCCATGTCCTTTG+TGG - chr4.1:8691034-8691053 MS.gene70243:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG + chr4.1:8690607-8690626 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG - chr4.1:8690664-8690683 MS.gene70243:CDS 40.0%
CACGTTCGATACAGTAATGA+TGG - chr4.1:8690833-8690852 MS.gene70243:CDS 40.0%
CCAATAAGAATCTTCTTCCC+TGG + chr4.1:8690271-8690290 None:intergenic 40.0%
CCAGGGAAGAAGATTCTTAT+TGG - chr4.1:8690268-8690287 MS.gene70243:CDS 40.0%
CTAAACAATGCCTCGTCTTT+AGG - chr4.1:8690405-8690424 MS.gene70243:CDS 40.0%
CTAGAGAATGAATTGTGTGG+TGG - chr4.1:8690210-8690229 MS.gene70243:CDS 40.0%
CTCACAGAAAAACTCCACAA+AGG + chr4.1:8691051-8691070 None:intergenic 40.0%
CTTGATCGAAGATTTCGTCT+AGG + chr4.1:8690113-8690132 None:intergenic 40.0%
GAAAAACTCCACAAAGGACA+TGG + chr4.1:8691045-8691064 None:intergenic 40.0%
GCGAAAATGTGTTTGTTTCC+AGG - chr4.1:8690250-8690269 MS.gene70243:CDS 40.0%
GTTAGCCCTTATAGAGAAAG+AGG - chr4.1:8691139-8691158 MS.gene70243:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG + chr4.1:8691130-8691149 None:intergenic 40.0%
TAGAGTAACAGCAATCACAG+TGG - chr4.1:8690734-8690753 MS.gene70243:CDS 40.0%
TCGACTAAAACAACAAGCCT+TGG + chr4.1:8690990-8691009 None:intergenic 40.0%
! AATGCCTCGTCTTTAGGAAA+AGG - chr4.1:8690411-8690430 MS.gene70243:CDS 40.0%
! CTGTGATTGCTGTTACTCTA+AGG + chr4.1:8690734-8690753 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG - chr4.1:8691049-8691068 MS.gene70243:CDS 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG - chr4.1:8691315-8691334 MS.gene70243:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG + chr4.1:8690979-8690998 None:intergenic 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG - chr4.1:8691351-8691370 MS.gene70243:CDS 40.0%
!! GCTCTAGAGAATGAATTGTG+TGG - chr4.1:8690207-8690226 MS.gene70243:CDS 40.0%
ATGAGATCTTCTGCTTGGAG+TGG + chr4.1:8690015-8690034 None:intergenic 45.0%
CAGTTCAACAAACAGGCAGA+AGG - chr4.1:8690775-8690794 MS.gene70243:CDS 45.0%
CTAAGCTACATTCATGCAGC+TGG + chr4.1:8689981-8690000 None:intergenic 45.0%
GAAGCCTCAGTTCAACAAAC+AGG - chr4.1:8690768-8690787 MS.gene70243:CDS 45.0%
GAGACATGAGATCTTCTGCT+TGG + chr4.1:8690020-8690039 None:intergenic 45.0%
TACATCAGTGGATGCCAAAC+AGG - chr4.1:8690916-8690935 MS.gene70243:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG - chr4.1:8691115-8691134 MS.gene70243:CDS 45.0%
TTGCTGCAGCAATTCTTGCA+TGG + chr4.1:8690142-8690161 None:intergenic 45.0%
! ATCGCCTTTTCCTAAAGACG+AGG + chr4.1:8690418-8690437 None:intergenic 45.0%
! CACATTTTCGCGGCCTTTTT+CGG + chr4.1:8690241-8690260 None:intergenic 45.0%
! TCTGCCTGTTTGTTGAACTG+AGG + chr4.1:8690775-8690794 None:intergenic 45.0%
! TGTGGTGGAGTTACCGAAAA+AGG - chr4.1:8690225-8690244 MS.gene70243:CDS 45.0%
! TTGAAGGGGTATAGACGTGT+TGG - chr4.1:8691251-8691270 MS.gene70243:CDS 45.0%
AATCCAATGGCCACCGTTGA+TGG - chr4.1:8690677-8690696 MS.gene70243:CDS 50.0%
AGATCTTCTGCTTGGAGTGG+TGG + chr4.1:8690012-8690031 None:intergenic 50.0%
GCAAAGTCAACCATCAACGG+TGG + chr4.1:8690690-8690709 None:intergenic 50.0%
! GCGATTTTCGAAGCTACAGC+TGG - chr4.1:8690612-8690631 MS.gene70243:CDS 50.0%
! TCATGCAGCTGGTTCATGCA+AGG + chr4.1:8689970-8689989 None:intergenic 50.0%
CAACCATCAACGGTGGCCAT+TGG + chr4.1:8690683-8690702 None:intergenic 55.0%
! CAGCTGGTTCATGCAAGGAG+GGG + chr4.1:8689965-8689984 None:intergenic 55.0%
! GCAGCTGGTTCATGCAAGGA+GGG + chr4.1:8689966-8689985 None:intergenic 55.0%
! TGCAGCTGGTTCATGCAAGG+AGG + chr4.1:8689967-8689986 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8689922 8691445 8689922 ID=MS.gene70243
chr4.1 mRNA 8689922 8691445 8689922 ID=MS.gene70243.t1;Parent=MS.gene70243
chr4.1 exon 8689922 8691445 8689922 ID=MS.gene70243.t1.exon1;Parent=MS.gene70243.t1
chr4.1 CDS 8689922 8691445 8689922 ID=cds.MS.gene70243.t1;Parent=MS.gene70243.t1
Gene Sequence

>MS.gene70243

ATGAATGTGATGGATTATGAGCAATTTGATTACTCATTCACCCCTCCTTGCATGAACCAGCTGCATGAATGTAGCTTAGAAAATTTACCACCACTCCAAGCAGAAGATCTCATGTCTCCAAATACAATCCTAGATGAAATATTGTTTGATCAAGACCAATCACAACATCTCTTATCTCCAAATACAATCCTAGACGAAATCTTCGATCAAGAAGAGTCCATGCAAGAATTGCTGCAGCAACATACAAACCTCGACTTCAATATCATGAATCATGAACAAGCAATGGCTCTAGAGAATGAATTGTGTGGTGGAGTTACCGAAAAAGGCCGCGAAAATGTGTTTGTTTCCAGGGAAGAAGATTCTTATTGGAAGGAAGTTCAAGAAGAGTTAATGGAAGAGACAAGTTTAGTTGATCTTTTGCTGATAGGAGCTGAAGCTGCTGAATCACAAAACATGACTCTTGCTTCTGACATAATCGAAAAACTAAACAATGCCTCGTCTTTAGGAAAAGGCGATAGTTTATTGAACAGGTTGTGTCTTTTCTTTACGCAAGGATTGTATTATAAAACTACAAATGCTCCTAAGTTCCATTCAGAACATGTTTCTACACAGACAAGCACATTCTGTGTGTTTCAGATACTTCAAGAACTCTCTCCCTATGTAAAATTTGCTCATTTTACTGCCAATCAAGCGATTTTCGAAGCTACAGCTGGTGTCGAAGATGTTCATGTCATTGATTTTGATATCATGGAGGGAATCCAATGGCCACCGTTGATGGTTGACTTTGCAATGAGAAAGAAGACTACTTCCCTTAGAGTAACAGCAATCACAGTGGATCAGCAAAGTGAAGCCTCAGTTCAACAAACAGGCAGAAGGCTCAAAGAGTTCGCTGATTCAATCAATTTTCCGTTCACGTTCGATACAGTAATGATGGTGAGTGAAGAAGAATTCAAAGAAATTGAACTTGGTGAAACATTTATTGTCAATTGTATGATACATCAGTGGATGCCAAACAGGAGTTTCTCATTGATCAAAGCTTTTCTCGATCACGTAACCAAATCATCACCAAGGCTTGTTGTTTTAGTCGAAGAAGAACTGTTTAATTTTTCTAGACTCAAATCCATGTCCTTTGTGGAGTTTTTCTGTGAGGCTTTGCATCACTATATTGCAGTTTCTGATTCACTTGTAAGTACTCTGTGGAGTAGCCATAAGATGGAGTTAGCCCTTATAGAGAAAGAGGTATTGGGAAATAGAATCTTAGACAGTGTAAGACAGTTTCCTTGTGAAAAAGAGGAGAGAATATTGTGGGAAGAAAGATTTTTTTATTCATTGAAGGGGTATAGACGTGTTGGTATGAGTACATGCAATATTTCACAGGCTAAGCTATTGGTTAGCTTGTTTGGTAAAGGGTATTGGGTGCAATATGAGAACTGTAAGTTGGCTTTGTGTTGGAAGTCAAGACCTTTGACTTCTGTTTCAATCTGGGTACCAACAGATTATAATATGAGTAACAAAGTCAAATAG
Protein sequence

>MS.gene70243.t1

MNVMDYEQFDYSFTPPCMNQLHECSLENLPPLQAEDLMSPNTILDEILFDQDQSQHLLSPNTILDEIFDQEESMQELLQQHTNLDFNIMNHEQAMALENELCGGVTEKGRENVFVSREEDSYWKEVQEELMEETSLVDLLLIGAEAAESQNMTLASDIIEKLNNASSLGKGDSLLNRLCLFFTQGLYYKTTNAPKFHSEHVSTQTSTFCVFQILQELSPYVKFAHFTANQAIFEATAGVEDVHVIDFDIMEGIQWPPLMVDFAMRKKTTSLRVTAITVDQQSEASVQQTGRRLKEFADSINFPFTFDTVMMVSEEEFKEIELGETFIVNCMIHQWMPNRSFSLIKAFLDHVTKSSPRLVVLVEEELFNFSRLKSMSFVEFFCEALHHYIAVSDSLVSTLWSSHKMELALIEKEVLGNRILDSVRQFPCEKEERILWEERFFYSLKGYRRVGMSTCNISQAKLLVSLFGKGYWVQYENCKLALCWKSRPLTSVSIWVPTDYNMSNKVK