Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene70246.t1 | AET04690.2 | 71.4 | 308 | 46 | 6 | 1 | 300 | 1 | 274 | 1.00E-103 | 386.7 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene70246.t1 | A2XE31 | 37.9 | 248 | 112 | 1 | 13 | 218 | 11 | 258 | 2.0e-39 | 164.5 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene70246.t1 | G7LD51 | 71.4 | 308 | 46 | 6 | 1 | 300 | 1 | 274 | 7.5e-104 | 386.7 |
TFs/TRs:
Gene ID | Type | Classification |
---|---|---|
MS.gene70246.t1 | TF | GARP-ARR-B |
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|
PPI:
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene70246.t1 | MTR_8g093040 | 71.429 | 308 | 46 | 6 | 1 | 300 | 1 | 274 | 2.56e-135 | 385 |
MS.gene70246.t1 | MTR_6g045327 | 53.674 | 313 | 117 | 5 | 1 | 303 | 1 | 295 | 6.42e-102 | 305 |
MS.gene70246.t1 | MTR_4g021760 | 48.476 | 328 | 136 | 8 | 1 | 313 | 1 | 310 | 6.73e-92 | 276 |
MS.gene70246.t1 | MTR_4g021855 | 50.996 | 251 | 100 | 5 | 1 | 240 | 1 | 239 | 3.49e-74 | 231 |
MS.gene70246.t1 | MTR_4g021790 | 44.983 | 289 | 127 | 6 | 1 | 278 | 1 | 268 | 1.40e-70 | 221 |
MS.gene70246.t1 | MTR_4g021845 | 50.901 | 222 | 93 | 4 | 1 | 211 | 1 | 217 | 2.99e-69 | 215 |
MS.gene70246.t1 | MTR_1g032570 | 37.467 | 379 | 161 | 9 | 12 | 320 | 9 | 381 | 4.86e-68 | 224 |
MS.gene70246.t1 | MTR_8g105600 | 37.216 | 352 | 163 | 10 | 14 | 324 | 9 | 343 | 5.27e-54 | 186 |
MS.gene70246.t1 | MTR_2g034960 | 42.083 | 240 | 105 | 2 | 13 | 218 | 28 | 267 | 4.24e-51 | 178 |
MS.gene70246.t1 | MTR_5g055260 | 45.494 | 233 | 86 | 7 | 1 | 223 | 1 | 202 | 1.33e-49 | 166 |
MS.gene70246.t1 | MTR_8g079940 | 38.462 | 260 | 121 | 3 | 14 | 237 | 25 | 281 | 5.60e-49 | 171 |
MS.gene70246.t1 | MTR_4g121020 | 40.083 | 242 | 109 | 2 | 13 | 218 | 30 | 271 | 1.94e-48 | 172 |
MS.gene70246.t1 | MTR_4g121020 | 40.083 | 242 | 109 | 2 | 13 | 218 | 30 | 271 | 2.04e-48 | 172 |
MS.gene70246.t1 | MTR_3g086100 | 37.500 | 272 | 130 | 4 | 14 | 247 | 13 | 282 | 6.64e-48 | 169 |
MS.gene70246.t1 | MTR_1g032570 | 34.906 | 318 | 131 | 10 | 73 | 320 | 1 | 312 | 3.36e-44 | 159 |
MS.gene70246.t1 | MTR_4g131600 | 35.968 | 253 | 129 | 2 | 4 | 223 | 10 | 262 | 2.19e-42 | 154 |
MS.gene70246.t1 | MTR_6g016850 | 47.134 | 157 | 70 | 4 | 9 | 153 | 4 | 159 | 1.13e-41 | 143 |
MS.gene70246.t1 | MTR_4g023980 | 50.735 | 136 | 56 | 2 | 61 | 185 | 36 | 171 | 9.59e-41 | 140 |
MS.gene70246.t1 | MTR_4g131580 | 33.840 | 263 | 132 | 3 | 14 | 234 | 17 | 279 | 1.94e-40 | 149 |
MS.gene70246.t1 | MTR_3g102600 | 36.574 | 216 | 113 | 1 | 13 | 228 | 7 | 198 | 5.24e-40 | 140 |
MS.gene70246.t1 | MTR_4g131570 | 35.458 | 251 | 124 | 3 | 14 | 226 | 17 | 267 | 2.03e-39 | 146 |
MS.gene70246.t1 | MTR_3g106220 | 35.081 | 248 | 119 | 3 | 13 | 218 | 7 | 254 | 2.67e-38 | 144 |
MS.gene70246.t1 | MTR_3g102600 | 37.186 | 199 | 101 | 1 | 13 | 211 | 7 | 181 | 3.48e-38 | 134 |
MS.gene70246.t1 | MTR_1g013170 | 34.836 | 244 | 121 | 2 | 13 | 218 | 4 | 247 | 3.39e-37 | 139 |
MS.gene70246.t1 | MTR_2g450070 | 31.559 | 263 | 133 | 3 | 6 | 221 | 10 | 272 | 2.32e-36 | 138 |
MS.gene70246.t1 | MTR_2g450070 | 31.559 | 263 | 133 | 3 | 6 | 221 | 10 | 272 | 2.61e-36 | 138 |
MS.gene70246.t1 | MTR_7g117705 | 33.469 | 245 | 125 | 3 | 13 | 219 | 4 | 248 | 1.40e-34 | 131 |
MS.gene70246.t1 | MTR_3g102590 | 36.139 | 202 | 114 | 3 | 13 | 213 | 7 | 194 | 5.95e-33 | 121 |
MS.gene70246.t1 | MTR_1g013180 | 32.422 | 256 | 135 | 2 | 1 | 218 | 143 | 398 | 6.55e-33 | 129 |
MS.gene70246.t1 | MTR_3g102590 | 36.318 | 201 | 113 | 3 | 13 | 212 | 7 | 193 | 8.11e-33 | 122 |
MS.gene70246.t1 | MTR_3g102600 | 36.416 | 173 | 86 | 1 | 56 | 228 | 1 | 149 | 1.41e-32 | 119 |
MS.gene70246.t1 | MTR_3g086100 | 35.681 | 213 | 97 | 4 | 73 | 247 | 1 | 211 | 1.81e-32 | 126 |
MS.gene70246.t1 | MTR_3g102600 | 37.179 | 156 | 74 | 1 | 56 | 211 | 1 | 132 | 6.64e-31 | 114 |
MS.gene70246.t1 | MTR_7g026400 | 33.500 | 200 | 105 | 3 | 44 | 218 | 17 | 213 | 7.80e-31 | 122 |
MS.gene70246.t1 | MTR_0450s0040 | 30.364 | 247 | 152 | 6 | 17 | 246 | 7 | 250 | 1.94e-29 | 115 |
MS.gene70246.t1 | MTR_4g121020 | 39.506 | 162 | 61 | 3 | 93 | 218 | 7 | 167 | 6.47e-25 | 105 |
MS.gene70246.t1 | MTR_8g032710 | 26.299 | 308 | 157 | 8 | 18 | 268 | 21 | 315 | 5.00e-21 | 93.6 |
MS.gene70246.t1 | MTR_1g067110 | 31.707 | 123 | 79 | 2 | 18 | 136 | 85 | 206 | 3.30e-14 | 73.9 |
MS.gene70246.t1 | MTR_1g067110 | 31.707 | 123 | 79 | 2 | 18 | 136 | 85 | 206 | 3.49e-14 | 73.6 |
MS.gene70246.t1 | MTR_4g061360 | 34.146 | 123 | 76 | 2 | 18 | 136 | 89 | 210 | 1.43e-13 | 72.0 |
MS.gene70246.t1 | MTR_8g024260 | 31.452 | 124 | 82 | 1 | 16 | 136 | 34 | 157 | 1.61e-13 | 71.6 |
MS.gene70246.t1 | MTR_7g118260 | 30.000 | 130 | 88 | 1 | 15 | 141 | 11 | 140 | 2.49e-13 | 70.9 |
MS.gene70246.t1 | MTR_3g092780 | 30.328 | 122 | 82 | 1 | 18 | 136 | 56 | 177 | 3.20e-13 | 70.9 |
MS.gene70246.t1 | MTR_3g092780 | 30.328 | 122 | 82 | 1 | 18 | 136 | 56 | 177 | 3.50e-13 | 70.5 |
MS.gene70246.t1 | MTR_3g092780 | 30.328 | 122 | 82 | 1 | 18 | 136 | 56 | 177 | 3.70e-13 | 70.5 |
MS.gene70246.t1 | MTR_3g092780 | 30.328 | 122 | 82 | 1 | 18 | 136 | 56 | 177 | 3.73e-13 | 70.5 |
MS.gene70246.t1 | MTR_7g118260 | 30.400 | 125 | 84 | 1 | 15 | 136 | 11 | 135 | 3.96e-13 | 70.5 |
MS.gene70246.t1 | MTR_4g064730 | 55.172 | 58 | 26 | 0 | 161 | 218 | 130 | 187 | 9.89e-13 | 68.2 |
MS.gene70246.t1 | MTR_5g014040 | 29.197 | 137 | 90 | 4 | 5 | 139 | 6 | 137 | 1.34e-11 | 65.9 |
MS.gene70246.t1 | MTR_5g041350 | 48.571 | 70 | 34 | 2 | 162 | 230 | 15 | 83 | 1.37e-11 | 65.1 |
MS.gene70246.t1 | MTR_5g014040 | 29.197 | 137 | 90 | 4 | 5 | 139 | 6 | 137 | 1.37e-11 | 65.5 |
MS.gene70246.t1 | MTR_7g089010 | 51.724 | 58 | 28 | 0 | 161 | 218 | 90 | 147 | 1.85e-11 | 64.3 |
MS.gene70246.t1 | MTR_4g098870 | 51.923 | 52 | 25 | 0 | 161 | 212 | 309 | 360 | 8.19e-11 | 63.2 |
MS.gene70246.t1 | MTR_4g098870 | 51.923 | 52 | 25 | 0 | 161 | 212 | 329 | 380 | 8.65e-11 | 63.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene70246.t1 | AT2G01760 | 37.548 | 261 | 118 | 2 | 13 | 228 | 6 | 266 | 5.65e-50 | 171 |
MS.gene70246.t1 | AT2G01760 | 33.910 | 289 | 118 | 3 | 13 | 228 | 6 | 294 | 3.11e-44 | 156 |
MS.gene70246.t1 | AT3G16857 | 34.831 | 267 | 118 | 2 | 8 | 218 | 27 | 293 | 2.88e-42 | 155 |
MS.gene70246.t1 | AT3G16857 | 34.831 | 267 | 118 | 2 | 8 | 218 | 27 | 293 | 2.99e-42 | 155 |
MS.gene70246.t1 | AT1G67710 | 38.095 | 252 | 117 | 5 | 15 | 227 | 8 | 259 | 2.61e-41 | 150 |
MS.gene70246.t1 | AT2G25180 | 36.508 | 252 | 125 | 4 | 1 | 218 | 1 | 251 | 4.04e-40 | 148 |
MS.gene70246.t1 | AT4G31920 | 37.860 | 243 | 122 | 5 | 1 | 218 | 1 | 239 | 3.40e-38 | 142 |
MS.gene70246.t1 | AT2G01760 | 35.323 | 201 | 85 | 2 | 73 | 228 | 1 | 201 | 2.02e-32 | 123 |
MS.gene70246.t1 | AT3G16857 | 33.632 | 223 | 92 | 2 | 52 | 218 | 1 | 223 | 2.73e-32 | 127 |
MS.gene70246.t1 | AT5G58080 | 30.962 | 239 | 131 | 3 | 13 | 218 | 13 | 250 | 2.27e-30 | 121 |
MS.gene70246.t1 | AT1G49190 | 29.688 | 256 | 137 | 5 | 5 | 218 | 21 | 275 | 7.98e-29 | 115 |
MS.gene70246.t1 | AT1G67710 | 37.113 | 194 | 83 | 5 | 73 | 227 | 1 | 194 | 1.40e-27 | 112 |
MS.gene70246.t1 | AT3G62670 | 31.381 | 239 | 125 | 6 | 19 | 224 | 42 | 274 | 9.27e-27 | 109 |
MS.gene70246.t1 | AT5G49240 | 28.125 | 256 | 135 | 3 | 3 | 215 | 27 | 276 | 6.33e-26 | 105 |
MS.gene70246.t1 | AT1G49190 | 35.878 | 131 | 84 | 0 | 6 | 136 | 22 | 152 | 9.08e-21 | 93.6 |
MS.gene70246.t1 | AT1G67710 | 35.632 | 174 | 73 | 5 | 93 | 227 | 12 | 185 | 8.46e-19 | 87.0 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 2.49e-16 | 80.1 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 2.63e-16 | 79.7 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 2.63e-16 | 79.7 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 2.63e-16 | 79.7 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 3.06e-16 | 79.3 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 3.06e-16 | 79.3 |
MS.gene70246.t1 | AT5G60100 | 33.607 | 122 | 78 | 1 | 18 | 136 | 64 | 185 | 3.06e-16 | 79.3 |
MS.gene70246.t1 | AT2G46790 | 32.520 | 123 | 80 | 1 | 18 | 137 | 37 | 159 | 4.31e-15 | 76.3 |
MS.gene70246.t1 | AT2G46790 | 32.520 | 123 | 80 | 1 | 18 | 137 | 37 | 159 | 5.16e-15 | 75.9 |
MS.gene70246.t1 | AT5G24470 | 31.148 | 122 | 81 | 1 | 18 | 136 | 50 | 171 | 2.90e-14 | 73.9 |
MS.gene70246.t1 | AT5G59570 | 54.237 | 59 | 27 | 0 | 160 | 218 | 139 | 197 | 1.61e-13 | 70.5 |
MS.gene70246.t1 | AT5G59570 | 54.237 | 59 | 27 | 0 | 160 | 218 | 139 | 197 | 1.61e-13 | 70.5 |
MS.gene70246.t1 | AT3G46640 | 55.172 | 58 | 26 | 0 | 161 | 218 | 143 | 200 | 4.11e-13 | 69.3 |
MS.gene70246.t1 | AT3G46640 | 55.172 | 58 | 26 | 0 | 161 | 218 | 143 | 200 | 4.11e-13 | 69.3 |
MS.gene70246.t1 | AT3G46640 | 55.172 | 58 | 26 | 0 | 161 | 218 | 143 | 200 | 4.50e-13 | 69.3 |
MS.gene70246.t1 | AT5G02810 | 31.148 | 122 | 81 | 1 | 18 | 136 | 78 | 199 | 5.11e-12 | 67.0 |
MS.gene70246.t1 | AT5G07210 | 39.423 | 104 | 61 | 2 | 132 | 234 | 199 | 301 | 5.32e-12 | 67.0 |
MS.gene70246.t1 | AT5G07210 | 39.423 | 104 | 61 | 2 | 132 | 234 | 191 | 293 | 5.84e-12 | 67.0 |
MS.gene70246.t1 | AT5G44190 | 53.704 | 54 | 25 | 0 | 159 | 212 | 144 | 197 | 7.38e-12 | 66.2 |
MS.gene70246.t1 | AT5G44190 | 53.704 | 54 | 25 | 0 | 159 | 212 | 144 | 197 | 7.76e-12 | 65.9 |
MS.gene70246.t1 | AT2G40970 | 50.000 | 58 | 29 | 0 | 161 | 218 | 104 | 161 | 8.09e-12 | 64.7 |
MS.gene70246.t1 | AT5G05090 | 51.724 | 58 | 28 | 0 | 161 | 218 | 80 | 137 | 9.46e-12 | 64.7 |
MS.gene70246.t1 | AT5G61380 | 29.508 | 122 | 83 | 1 | 18 | 136 | 19 | 140 | 1.31e-11 | 65.9 |
MS.gene70246.t1 | AT3G10760 | 51.724 | 58 | 28 | 0 | 161 | 218 | 104 | 161 | 1.34e-11 | 65.1 |
MS.gene70246.t1 | AT3G04030 | 52.727 | 55 | 25 | 1 | 162 | 215 | 45 | 99 | 7.65e-11 | 63.2 |
MS.gene70246.t1 | AT3G04030 | 52.727 | 55 | 25 | 1 | 162 | 215 | 45 | 99 | 8.15e-11 | 63.2 |
Find 55 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TTGATTTGATTCTCGATTAA+AGG | 0.164354 | 4.1:-8717887 | None:intergenic |
GGAATATGGTATATTCAATT+AGG | 0.202842 | 4.1:+8718690 | MS.gene70246:CDS |
AATTGCATCGACAATTTCTA+AGG | 0.211162 | 4.1:+8718038 | MS.gene70246:CDS |
TGTTTCCATCAAAAGTTTAA+AGG | 0.235719 | 4.1:-8717570 | None:intergenic |
CAAACGGGGCTTGCGAATAT+TGG | 0.275266 | 4.1:+8717858 | MS.gene70246:CDS |
TCCAGTTACAACATGTTCTA+AGG | 0.282036 | 4.1:+8717538 | MS.gene70246:intron |
ATCGCTTTATGAATTGCTTT+TGG | 0.284723 | 4.1:-8718202 | None:intergenic |
TAAAGAATAGAAAGTCAAAA+AGG | 0.285756 | 4.1:+8718407 | MS.gene70246:CDS |
ATTCTTTAGATGTGTCTCTT+TGG | 0.286927 | 4.1:-8718392 | None:intergenic |
ACATTTCCACAACAGCATTA+AGG | 0.328612 | 4.1:-8717367 | None:intergenic |
AGAAATTGTCGATGCAATTC+AGG | 0.341097 | 4.1:-8718034 | None:intergenic |
GCACAACCTCCATCGGAATA+TGG | 0.347834 | 4.1:+8718676 | MS.gene70246:CDS |
CTCTTAAATCCTCTGGGAAT+TGG | 0.352622 | 4.1:-8717316 | None:intergenic |
GCAAGTTGCTAGCCATTTGC+AGG | 0.355161 | 4.1:+8718249 | MS.gene70246:CDS |
CTAAGGGTTGTGAATGAACT+TGG | 0.358456 | 4.1:+8718055 | MS.gene70246:CDS |
CATAAAGCGATGAATGTTCC+TGG | 0.373937 | 4.1:+8718214 | MS.gene70246:CDS |
ATATACGATTCTTCTTCGTT+AGG | 0.374538 | 4.1:-8718003 | None:intergenic |
TATACGATTCTTCTTCGTTA+GGG | 0.386785 | 4.1:-8718002 | None:intergenic |
GAATATGGTATATTCAATTA+GGG | 0.387647 | 4.1:+8718691 | MS.gene70246:CDS |
GAACTCAATTGTGTTTCGTT+AGG | 0.423423 | 4.1:-8718466 | None:intergenic |
ACAAGAACTCTTAAATCCTC+TGG | 0.429240 | 4.1:-8717323 | None:intergenic |
AACAAACCTTGATAATTGCT+TGG | 0.435234 | 4.1:-8717395 | None:intergenic |
AGAGTTGTCGTTGCATTGTA+TGG | 0.450449 | 4.1:-8718539 | None:intergenic |
TGACAGGAATATTGATTTGT+TGG | 0.454324 | 4.1:-8717670 | None:intergenic |
TAAATCCTCTGGGAATTGGC+TGG | 0.455635 | 4.1:-8717312 | None:intergenic |
TGATGAAAGCTGTTGCAAAC+GGG | 0.461960 | 4.1:+8717843 | MS.gene70246:CDS |
ATAAACATAATCAAAGCAAA+AGG | 0.469172 | 4.1:+8717951 | MS.gene70246:CDS |
TCTATTCCAAGCAATTATCA+AGG | 0.472629 | 4.1:+8717389 | MS.gene70246:CDS |
AAATCATATGAATCCATGTC+TGG | 0.476246 | 4.1:-8717632 | None:intergenic |
TAAACATAATCAAAGCAAAA+GGG | 0.478480 | 4.1:+8717952 | MS.gene70246:CDS |
GTGATGAAAGCTGTTGCAAA+CGG | 0.482441 | 4.1:+8717842 | MS.gene70246:CDS |
TATATATTCTTACTGATGAC+AGG | 0.490720 | 4.1:-8717686 | None:intergenic |
GACAGGAATATTGATTTGTT+GGG | 0.492437 | 4.1:-8717669 | None:intergenic |
CGAAGAAGAATCGTATATCA+TGG | 0.494656 | 4.1:+8718008 | MS.gene70246:CDS |
TGATTTGTTGGGTAACATGT+CGG | 0.535639 | 4.1:-8717658 | None:intergenic |
ATAGTGATGATGAATCATCT+TGG | 0.538208 | 4.1:-8718648 | None:intergenic |
AGAGGATTTAAGAGTTCTTG+TGG | 0.539349 | 4.1:+8717325 | MS.gene70246:CDS |
CAAGAACTCTTAAATCCTCT+GGG | 0.542866 | 4.1:-8717322 | None:intergenic |
CATCTTGGATCATGTAGGAC+TGG | 0.543187 | 4.1:-8718633 | None:intergenic |
TGAAGCTCAAATGCCAGACA+TGG | 0.549847 | 4.1:+8717619 | MS.gene70246:CDS |
TGTGCAGTGATGTGTGATGA+TGG | 0.552839 | 4.1:+8717806 | MS.gene70246:intron |
AAATCCTCTGGGAATTGGCT+GGG | 0.555538 | 4.1:-8717311 | None:intergenic |
TGATGTCCTTAATGCTGTTG+TGG | 0.557832 | 4.1:+8717361 | MS.gene70246:CDS |
ATACGAAGCACAACCTCCAT+CGG | 0.580105 | 4.1:+8718669 | MS.gene70246:CDS |
ATACGATTCTTCTTCGTTAG+GGG | 0.588904 | 4.1:-8718001 | None:intergenic |
AATATACCATATTCCGATGG+AGG | 0.592529 | 4.1:-8718682 | None:intergenic |
ATTGCATCGACAATTTCTAA+GGG | 0.597352 | 4.1:+8718039 | MS.gene70246:CDS |
AGAATCAAATCAAGAACATG+TGG | 0.615053 | 4.1:+8717897 | MS.gene70246:CDS |
GATGAAAGCTGTTGCAAACG+GGG | 0.621578 | 4.1:+8717844 | MS.gene70246:CDS |
AAACATAATCAAAGCAAAAG+GGG | 0.630491 | 4.1:+8717953 | MS.gene70246:CDS |
TGAATCATCTTGGATCATGT+AGG | 0.631847 | 4.1:-8718638 | None:intergenic |
TTGAATATACCATATTCCGA+TGG | 0.640230 | 4.1:-8718685 | None:intergenic |
AACAAAGGGCTGTGTTGATG+TGG | 0.643059 | 4.1:+8717589 | MS.gene70246:CDS |
ACATGTGGCAGTGTGTTGCA+AGG | 0.672995 | 4.1:+8717912 | MS.gene70246:CDS |
ACTTCCCAGCCAATTCCCAG+AGG | 0.715638 | 4.1:+8717307 | MS.gene70246:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | AGAGAGAAAATAAAAATTAT+TGG | - | chr4.1:8718103-8718122 | None:intergenic | 15.0% |
!! | AAAGAATAGAAAGTCAAAAA+GGG | + | chr4.1:8718408-8718427 | MS.gene70246:CDS | 20.0% |
!! | ATAAACATAATCAAAGCAAA+AGG | + | chr4.1:8717951-8717970 | MS.gene70246:CDS | 20.0% |
!! | TAAACATAATCAAAGCAAAA+GGG | + | chr4.1:8717952-8717971 | MS.gene70246:CDS | 20.0% |
!! | TAAAGAATAGAAAGTCAAAA+AGG | + | chr4.1:8718407-8718426 | MS.gene70246:CDS | 20.0% |
!!! | TGTTTTTTGTATTAATGTAG+TGG | + | chr4.1:8718274-8718293 | MS.gene70246:intron | 20.0% |
! | AAACATAATCAAAGCAAAAG+GGG | + | chr4.1:8717953-8717972 | MS.gene70246:CDS | 25.0% |
! | AAGAATAGAAAGTCAAAAAG+GGG | + | chr4.1:8718409-8718428 | MS.gene70246:CDS | 25.0% |
! | GAAAAAGAATCTTACTATCA+AGG | - | chr4.1:8718081-8718100 | None:intergenic | 25.0% |
! | TATATATTCTTACTGATGAC+AGG | - | chr4.1:8717689-8717708 | None:intergenic | 25.0% |
! | TGTTTCCATCAAAAGTTTAA+AGG | - | chr4.1:8717573-8717592 | None:intergenic | 25.0% |
!! | AAACTTTTGATGGAAACAAA+GGG | + | chr4.1:8717575-8717594 | MS.gene70246:CDS | 25.0% |
!! | CTGAGTTCATTAAATTTTCT+GGG | - | chr4.1:8718519-8718538 | None:intergenic | 25.0% |
!! | TAAACTTTTGATGGAAACAA+AGG | + | chr4.1:8717574-8717593 | MS.gene70246:CDS | 25.0% |
!! | TTGATTTGATTCTCGATTAA+AGG | - | chr4.1:8717890-8717909 | None:intergenic | 25.0% |
!! | TTTTGCCTTTAAACTTTTGA+TGG | + | chr4.1:8717565-8717584 | MS.gene70246:CDS | 25.0% |
AAATCATATGAATCCATGTC+TGG | - | chr4.1:8717635-8717654 | None:intergenic | 30.0% | |
ACAAAAAACAAACCTGCAAA+TGG | - | chr4.1:8718264-8718283 | None:intergenic | 30.0% | |
AGAATAGAAAGTCAAAAAGG+GGG | + | chr4.1:8718410-8718429 | MS.gene70246:CDS | 30.0% | |
AGAATCAAATCAAGAACATG+TGG | + | chr4.1:8717897-8717916 | MS.gene70246:CDS | 30.0% | |
ATATACGATTCTTCTTCGTT+AGG | - | chr4.1:8718006-8718025 | None:intergenic | 30.0% | |
ATTCTTTAGATGTGTCTCTT+TGG | - | chr4.1:8718395-8718414 | None:intergenic | 30.0% | |
CAAAAGTTCAAGAATTTCAC+AGG | - | chr4.1:8717474-8717493 | None:intergenic | 30.0% | |
TATACGATTCTTCTTCGTTA+GGG | - | chr4.1:8718005-8718024 | None:intergenic | 30.0% | |
TCTATTCCAAGCAATTATCA+AGG | + | chr4.1:8717389-8717408 | MS.gene70246:CDS | 30.0% | |
TTGAATATACCATATTCCGA+TGG | - | chr4.1:8718688-8718707 | None:intergenic | 30.0% | |
! | AACAAACCTTGATAATTGCT+TGG | - | chr4.1:8717398-8717417 | None:intergenic | 30.0% |
! | AATTGCATCGACAATTTCTA+AGG | + | chr4.1:8718038-8718057 | MS.gene70246:CDS | 30.0% |
! | ATAGTGATGATGAATCATCT+TGG | - | chr4.1:8718651-8718670 | None:intergenic | 30.0% |
! | ATCGCTTTATGAATTGCTTT+TGG | - | chr4.1:8718205-8718224 | None:intergenic | 30.0% |
! | ATTGCATCGACAATTTCTAA+GGG | + | chr4.1:8718039-8718058 | MS.gene70246:CDS | 30.0% |
! | CTGTGAAATTCTTGAACTTT+TGG | + | chr4.1:8717472-8717491 | MS.gene70246:intron | 30.0% |
! | GCTGAGTTCATTAAATTTTC+TGG | - | chr4.1:8718520-8718539 | None:intergenic | 30.0% |
!! | GACAGGAATATTGATTTGTT+GGG | - | chr4.1:8717672-8717691 | None:intergenic | 30.0% |
!! | TGACAGGAATATTGATTTGT+TGG | - | chr4.1:8717673-8717692 | None:intergenic | 30.0% |
AATATACCATATTCCGATGG+AGG | - | chr4.1:8718685-8718704 | None:intergenic | 35.0% | |
ACAAGAACTCTTAAATCCTC+TGG | - | chr4.1:8717326-8717345 | None:intergenic | 35.0% | |
ACATTTCCACAACAGCATTA+AGG | - | chr4.1:8717370-8717389 | None:intergenic | 35.0% | |
AGAAATTGTCGATGCAATTC+AGG | - | chr4.1:8718037-8718056 | None:intergenic | 35.0% | |
AGAGGATTTAAGAGTTCTTG+TGG | + | chr4.1:8717325-8717344 | MS.gene70246:CDS | 35.0% | |
ATACGATTCTTCTTCGTTAG+GGG | - | chr4.1:8718004-8718023 | None:intergenic | 35.0% | |
CAAGAACTCTTAAATCCTCT+GGG | - | chr4.1:8717325-8717344 | None:intergenic | 35.0% | |
CGAAGAAGAATCGTATATCA+TGG | + | chr4.1:8718008-8718027 | MS.gene70246:CDS | 35.0% | |
GAACTCAATTGTGTTTCGTT+AGG | - | chr4.1:8718469-8718488 | None:intergenic | 35.0% | |
GGAGAGACAAAAATTTACAC+AGG | - | chr4.1:8718162-8718181 | None:intergenic | 35.0% | |
TAGAACATGTTGTAACTGGA+AGG | - | chr4.1:8717538-8717557 | None:intergenic | 35.0% | |
TCCAGTTACAACATGTTCTA+AGG | + | chr4.1:8717538-8717557 | MS.gene70246:intron | 35.0% | |
TGAATCATCTTGGATCATGT+AGG | - | chr4.1:8718641-8718660 | None:intergenic | 35.0% | |
! | GCTTTGCCTCTGAAAAATAA+GGG | - | chr4.1:8718183-8718202 | None:intergenic | 35.0% |
!! | TGATTTGTTGGGTAACATGT+CGG | - | chr4.1:8717661-8717680 | None:intergenic | 35.0% |
AGAGTTGTCGTTGCATTGTA+TGG | - | chr4.1:8718542-8718561 | None:intergenic | 40.0% | |
ATAGAAAGTCAAAAAGGGGG+CGG | + | chr4.1:8718413-8718432 | MS.gene70246:CDS | 40.0% | |
CATAAAGCGATGAATGTTCC+TGG | + | chr4.1:8718214-8718233 | MS.gene70246:CDS | 40.0% | |
CTAAGGGTTGTGAATGAACT+TGG | + | chr4.1:8718055-8718074 | MS.gene70246:CDS | 40.0% | |
GCCTTAGAACATGTTGTAAC+TGG | - | chr4.1:8717542-8717561 | None:intergenic | 40.0% | |
GTGATGAAAGCTGTTGCAAA+CGG | + | chr4.1:8717842-8717861 | MS.gene70246:CDS | 40.0% | |
TGATGAAAGCTGTTGCAAAC+GGG | + | chr4.1:8717843-8717862 | MS.gene70246:CDS | 40.0% | |
TGATGTCCTTAATGCTGTTG+TGG | + | chr4.1:8717361-8717380 | MS.gene70246:CDS | 40.0% | |
! | ACTTGCTCTTTTGTCAAACC+AGG | - | chr4.1:8718235-8718254 | None:intergenic | 40.0% |
! | CTCTTAAATCCTCTGGGAAT+TGG | - | chr4.1:8717319-8717338 | None:intergenic | 40.0% |
! | GGCTTTGCCTCTGAAAAATA+AGG | - | chr4.1:8718184-8718203 | None:intergenic | 40.0% |
!! | GGACTGGTCAGTAAAAATGT+TGG | - | chr4.1:8718620-8718639 | None:intergenic | 40.0% |
!!! | GTCTCTCCCTTATTTTTCAG+AGG | + | chr4.1:8718174-8718193 | MS.gene70246:intron | 40.0% |
ATACGAAGCACAACCTCCAT+CGG | + | chr4.1:8718669-8718688 | MS.gene70246:CDS | 45.0% | |
CATCTTGGATCATGTAGGAC+TGG | - | chr4.1:8718636-8718655 | None:intergenic | 45.0% | |
GATGAAAGCTGTTGCAAACG+GGG | + | chr4.1:8717844-8717863 | MS.gene70246:CDS | 45.0% | |
TGAAGCTCAAATGCCAGACA+TGG | + | chr4.1:8717619-8717638 | MS.gene70246:CDS | 45.0% | |
TGTGCAGTGATGTGTGATGA+TGG | + | chr4.1:8717806-8717825 | MS.gene70246:intron | 45.0% | |
! | AAATCCTCTGGGAATTGGCT+GGG | - | chr4.1:8717314-8717333 | None:intergenic | 45.0% |
! | AACAAAGGGCTGTGTTGATG+TGG | + | chr4.1:8717589-8717608 | MS.gene70246:CDS | 45.0% |
! | TAAATCCTCTGGGAATTGGC+TGG | - | chr4.1:8717315-8717334 | None:intergenic | 45.0% |
ACATGTGGCAGTGTGTTGCA+AGG | + | chr4.1:8717912-8717931 | MS.gene70246:CDS | 50.0% | |
CAAACGGGGCTTGCGAATAT+TGG | + | chr4.1:8717858-8717877 | MS.gene70246:CDS | 50.0% | |
GCAAGTTGCTAGCCATTTGC+AGG | + | chr4.1:8718249-8718268 | MS.gene70246:CDS | 50.0% | |
GCACAACCTCCATCGGAATA+TGG | + | chr4.1:8718676-8718695 | MS.gene70246:CDS | 50.0% | |
ACTTCCCAGCCAATTCCCAG+AGG | + | chr4.1:8717307-8717326 | MS.gene70246:CDS | 55.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr4.1 | gene | 8717281 | 8718711 | 8717281 | ID=MS.gene70246 |
chr4.1 | mRNA | 8717281 | 8718711 | 8717281 | ID=MS.gene70246.t1;Parent=MS.gene70246 |
chr4.1 | exon | 8717281 | 8717410 | 8717281 | ID=MS.gene70246.t1.exon1;Parent=MS.gene70246.t1 |
chr4.1 | CDS | 8717281 | 8717410 | 8717281 | ID=cds.MS.gene70246.t1;Parent=MS.gene70246.t1 |
chr4.1 | exon | 8717543 | 8717695 | 8717543 | ID=MS.gene70246.t1.exon2;Parent=MS.gene70246.t1 |
chr4.1 | CDS | 8717543 | 8717695 | 8717543 | ID=cds.MS.gene70246.t1;Parent=MS.gene70246.t1 |
chr4.1 | exon | 8717813 | 8718085 | 8717813 | ID=MS.gene70246.t1.exon3;Parent=MS.gene70246.t1 |
chr4.1 | CDS | 8717813 | 8718085 | 8717813 | ID=cds.MS.gene70246.t1;Parent=MS.gene70246.t1 |
chr4.1 | exon | 8718194 | 8718270 | 8718194 | ID=MS.gene70246.t1.exon4;Parent=MS.gene70246.t1 |
chr4.1 | CDS | 8718194 | 8718270 | 8718194 | ID=cds.MS.gene70246.t1;Parent=MS.gene70246.t1 |
chr4.1 | exon | 8718364 | 8718711 | 8718364 | ID=MS.gene70246.t1.exon5;Parent=MS.gene70246.t1 |
chr4.1 | CDS | 8718364 | 8718711 | 8718364 | ID=cds.MS.gene70246.t1;Parent=MS.gene70246.t1 |
Gene Sequence |
Protein sequence |