Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70510.t1 | XP_013461146.1 | 78.9 | 76 | 5 | 1 | 16 | 80 | 114 | 189 | 1.70E-23 | 118.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70510.t1 | Q75LL6 | 45.7 | 70 | 27 | 1 | 10 | 68 | 112 | 181 | 4.1e-10 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70510.t1 | A0A072VAF9 | 78.9 | 76 | 5 | 1 | 16 | 80 | 114 | 189 | 1.2e-23 | 118.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70510.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70510.t1 | MTR_3g081380 | 78.947 | 76 | 5 | 1 | 16 | 80 | 114 | 189 | 2.83e-36 | 120 |
| MS.gene70510.t1 | MTR_3g081360 | 64.789 | 71 | 14 | 1 | 16 | 75 | 101 | 171 | 1.52e-22 | 89.7 |
| MS.gene70510.t1 | MTR_3g081390 | 61.667 | 60 | 12 | 1 | 22 | 70 | 67 | 126 | 2.72e-17 | 70.9 |
| MS.gene70510.t1 | MTR_3g082790 | 56.250 | 64 | 17 | 1 | 22 | 74 | 125 | 188 | 1.27e-16 | 72.8 |
| MS.gene70510.t1 | MTR_3g082790 | 56.250 | 64 | 17 | 1 | 22 | 74 | 85 | 148 | 1.69e-16 | 72.4 |
| MS.gene70510.t1 | MTR_2g026380 | 42.857 | 70 | 29 | 1 | 10 | 68 | 109 | 178 | 9.73e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70510.t1 | AT3G07740 | 42.667 | 75 | 32 | 1 | 10 | 73 | 41 | 115 | 2.13e-12 | 60.8 |
| MS.gene70510.t1 | AT3G07740 | 42.667 | 75 | 32 | 1 | 10 | 73 | 112 | 186 | 2.22e-12 | 60.8 |
| MS.gene70510.t1 | AT3G07740 | 42.667 | 75 | 32 | 1 | 10 | 73 | 119 | 193 | 2.31e-12 | 60.8 |
| MS.gene70510.t1 | AT3G07740 | 42.667 | 75 | 32 | 1 | 10 | 73 | 91 | 165 | 2.63e-12 | 60.5 |
| MS.gene70510.t1 | AT4G16420 | 45.312 | 64 | 24 | 1 | 16 | 68 | 112 | 175 | 3.99e-12 | 60.1 |
| MS.gene70510.t1 | AT4G16420 | 45.312 | 64 | 24 | 1 | 16 | 68 | 112 | 175 | 4.07e-12 | 60.1 |
| MS.gene70510.t1 | AT4G16420 | 45.312 | 64 | 24 | 1 | 16 | 68 | 112 | 175 | 4.15e-12 | 60.1 |
Find 20 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAATGCATTAACAAATA+AGG | 0.295574 | 3.1:-67127343 | MS.gene70510:CDS |
| TTGCTCTTTGTTCTTTCCCT+TGG | 0.303043 | 3.1:+67126848 | None:intergenic |
| ACATGTATGGATTTGAGAAT+TGG | 0.349491 | 3.1:-67127063 | MS.gene70510:CDS |
| TAATGCAGGGGATAAAGCTT+TGG | 0.419580 | 3.1:-67126368 | MS.gene70510:intron |
| GTAGGATTTGTCTTATTCCA+AGG | 0.468148 | 3.1:-67126865 | MS.gene70510:intron |
| TCTCAAATCCATACATGTCA+AGG | 0.519033 | 3.1:+67127068 | None:intergenic |
| GAGTTGATCTCGCTTTCCCA+TGG | 0.527997 | 3.1:+67126818 | None:intergenic |
| ATGGTGTTGCTGAACATGTT+GGG | 0.532190 | 3.1:-67127039 | MS.gene70510:CDS |
| CAAAGAGCAACTCCGTGCCA+TGG | 0.534697 | 3.1:-67126835 | MS.gene70510:CDS |
| TAGGATTTGTCTTATTCCAA+GGG | 0.536827 | 3.1:-67126864 | MS.gene70510:CDS |
| AAAGAGCAACTCCGTGCCAT+GGG | 0.560848 | 3.1:-67126834 | MS.gene70510:CDS |
| AAGCGAGATCAACTCAAGAA+AGG | 0.572505 | 3.1:-67126810 | MS.gene70510:intron |
| TCAGATATACCACATTATAG+TGG | 0.585833 | 3.1:+67126990 | None:intergenic |
| TATGGATTTGAGAATTGGAA+TGG | 0.599113 | 3.1:-67127058 | MS.gene70510:CDS |
| CTCAAATCCATACATGTCAA+GGG | 0.602788 | 3.1:+67127069 | None:intergenic |
| AATGGTGTTGCTGAACATGT+TGG | 0.605755 | 3.1:-67127040 | MS.gene70510:CDS |
| GATCTCGCTTTCCCATGGCA+CGG | 0.612939 | 3.1:+67126823 | None:intergenic |
| TAGAGCAGAATTGATACATG+AGG | 0.674226 | 3.1:-67127319 | MS.gene70510:intron |
| TTATAGTGGTCAATACACTC+AGG | 0.688875 | 3.1:+67127004 | None:intergenic |
| GTGTATTGACCACTATAATG+TGG | 0.742988 | 3.1:-67126999 | MS.gene70510:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTATGATAATATGAAATA+AGG | + | chr3.1:67126610-67126629 | None:intergenic | 15.0% |
| !! | AGAATGAAAATTTCTTTGAT+AGG | - | chr3.1:67127205-67127224 | MS.gene70510:intron | 20.0% |
| !! | ATTTGTAGAAATAGACAAAT+AGG | - | chr3.1:67127055-67127074 | MS.gene70510:CDS | 20.0% |
| !! | TGAAAATGCATTAACAAATA+AGG | - | chr3.1:67126374-67126393 | MS.gene70510:intron | 20.0% |
| !!! | AAACCAAACTTTTTTTTTAG+AGG | - | chr3.1:67127016-67127035 | MS.gene70510:CDS | 20.0% |
| !!! | GGGATTTTATTTTTTTGTAA+TGG | - | chr3.1:67127287-67127306 | MS.gene70510:intron | 20.0% |
| ! | TTCATTCTTTACCTAATTTC+AGG | + | chr3.1:67127193-67127212 | None:intergenic | 25.0% |
| !! | AAAAAAAGTTTGGTTTCGTT+TGG | + | chr3.1:67127012-67127031 | None:intergenic | 25.0% |
| !! | TTATGACATTGATTAATGCA+GGG | - | chr3.1:67127336-67127355 | MS.gene70510:CDS | 25.0% |
| !! | TTTATGACATTGATTAATGC+AGG | - | chr3.1:67127335-67127354 | MS.gene70510:CDS | 25.0% |
| AAACTTTATCCCACGAAATA+GGG | - | chr3.1:67126973-67126992 | MS.gene70510:intron | 30.0% | |
| AAGCCAATCAGATAAAATGA+AGG | + | chr3.1:67127317-67127336 | None:intergenic | 30.0% | |
| ACATGTATGGATTTGAGAAT+TGG | - | chr3.1:67126654-67126673 | MS.gene70510:intron | 30.0% | |
| ACGTTGTCAAATAGACTTAA+AGG | + | chr3.1:67126773-67126792 | None:intergenic | 30.0% | |
| ATGATCTAAATCACAATGTG+TGG | - | chr3.1:67126941-67126960 | MS.gene70510:intron | 30.0% | |
| ATGTGCACAATCAATTTAAC+TGG | - | chr3.1:67127166-67127185 | MS.gene70510:intron | 30.0% | |
| ATTTAACTGGACCTGAAATT+AGG | - | chr3.1:67127179-67127198 | MS.gene70510:intron | 30.0% | |
| GTGTAATTGTTTGTACTTGT+AGG | - | chr3.1:67126834-67126853 | MS.gene70510:CDS | 30.0% | |
| TATGGATTTGAGAATTGGAA+TGG | - | chr3.1:67126659-67126678 | MS.gene70510:intron | 30.0% | |
| TCAAATAGACTTAAAGGAAG+AGG | + | chr3.1:67126767-67126786 | None:intergenic | 30.0% | |
| TCAGATATACCACATTATAG+TGG | + | chr3.1:67126730-67126749 | None:intergenic | 30.0% | |
| ! | CCACCTCTAAAAAAAAAGTT+TGG | + | chr3.1:67127022-67127041 | None:intergenic | 30.0% |
| ! | TAGGATTTGTCTTATTCCAA+GGG | - | chr3.1:67126853-67126872 | MS.gene70510:CDS | 30.0% |
| ! | TATGACATTGATTAATGCAG+GGG | - | chr3.1:67127337-67127356 | MS.gene70510:CDS | 30.0% |
| !! | CTTTATTAGCTTTGAGACAA+TGG | - | chr3.1:67127263-67127282 | MS.gene70510:intron | 30.0% |
| !! | TAACAAGCTTGTTTCGTTTT+AGG | - | chr3.1:67126625-67126644 | MS.gene70510:intron | 30.0% |
| !!! | CCAAACTTTTTTTTTAGAGG+TGG | - | chr3.1:67127019-67127038 | MS.gene70510:CDS | 30.0% |
| CAAACTTTATCCCACGAAAT+AGG | - | chr3.1:67126972-67126991 | MS.gene70510:intron | 35.0% | |
| CTCAAATCCATACATGTCAA+GGG | + | chr3.1:67126651-67126670 | None:intergenic | 35.0% | |
| TAGAGCAGAATTGATACATG+AGG | - | chr3.1:67126398-67126417 | MS.gene70510:intron | 35.0% | |
| TCTCAAATCCATACATGTCA+AGG | + | chr3.1:67126652-67126671 | None:intergenic | 35.0% | |
| TTATAGTGGTCAATACACTC+AGG | + | chr3.1:67126716-67126735 | None:intergenic | 35.0% | |
| TTCTGCTTACATGTCCATAT+TGG | - | chr3.1:67126475-67126494 | MS.gene70510:intron | 35.0% | |
| ! | CTGCCTTCATTTTATCTGAT+TGG | - | chr3.1:67127311-67127330 | MS.gene70510:intron | 35.0% |
| ! | GTAGGATTTGTCTTATTCCA+AGG | - | chr3.1:67126852-67126871 | MS.gene70510:CDS | 35.0% |
| ! | GTGTATTGACCACTATAATG+TGG | - | chr3.1:67126718-67126737 | MS.gene70510:intron | 35.0% |
| !! | ATTAGCTTTGAGACAATGGT+GGG | - | chr3.1:67127267-67127286 | MS.gene70510:intron | 35.0% |
| !! | TATTAGCTTTGAGACAATGG+TGG | - | chr3.1:67127266-67127285 | MS.gene70510:intron | 35.0% |
| AAGCGAGATCAACTCAAGAA+AGG | - | chr3.1:67126907-67126926 | MS.gene70510:intron | 40.0% | |
| AGGTTTGTGGTTTGAGTATG+GGG | - | chr3.1:67126526-67126545 | MS.gene70510:intron | 40.0% | |
| ATACTCAAACCACAAACCTC+AGG | + | chr3.1:67126525-67126544 | None:intergenic | 40.0% | |
| ATATAGCCAACCCTATTTCG+TGG | + | chr3.1:67126986-67127005 | None:intergenic | 40.0% | |
| GAGGTTTGTGGTTTGAGTAT+GGG | - | chr3.1:67126525-67126544 | MS.gene70510:intron | 40.0% | |
| TAATGCAGGGGATAAAGCTT+TGG | - | chr3.1:67127349-67127368 | MS.gene70510:CDS | 40.0% | |
| TACACCAAGCATTCAAACAG+AGG | + | chr3.1:67126819-67126838 | None:intergenic | 40.0% | |
| TATAGCCAACCCTATTTCGT+GGG | + | chr3.1:67126985-67127004 | None:intergenic | 40.0% | |
| TCTTCATGCACACTCCAATA+TGG | + | chr3.1:67126492-67126511 | None:intergenic | 40.0% | |
| TGAGGTTTGTGGTTTGAGTA+TGG | - | chr3.1:67126524-67126543 | MS.gene70510:intron | 40.0% | |
| TTGCTCTTTGTTCTTTCCCT+TGG | + | chr3.1:67126872-67126891 | None:intergenic | 40.0% | |
| TTTATCCCACGAAATAGGGT+TGG | - | chr3.1:67126977-67126996 | MS.gene70510:intron | 40.0% | |
| ! | TGAAGAGTTGTTACTTCCTG+AGG | - | chr3.1:67126506-67126525 | MS.gene70510:intron | 40.0% |
| ! | TTGTTACTTCCTGAGGTTTG+TGG | - | chr3.1:67126513-67126532 | MS.gene70510:intron | 40.0% |
| ! | TTTTAGGCCCTTGACATGTA+TGG | - | chr3.1:67126641-67126660 | MS.gene70510:intron | 40.0% |
| !! | AATGGTGTTGCTGAACATGT+TGG | - | chr3.1:67126677-67126696 | MS.gene70510:intron | 40.0% |
| !! | ACGAAATAGGGTTGGCTATA+TGG | - | chr3.1:67126985-67127004 | MS.gene70510:intron | 40.0% |
| !! | ATGGTGTTGCTGAACATGTT+GGG | - | chr3.1:67126678-67126697 | MS.gene70510:intron | 40.0% |
| !! | TCGACAACGAAAGCATTTGA+AGG | - | chr3.1:67127118-67127137 | MS.gene70510:intron | 40.0% |
| GCAACCTCTGTTTGAATGCT+TGG | - | chr3.1:67126812-67126831 | MS.gene70510:CDS | 45.0% | |
| ! | CTTAAAGGAAGAGGAGCACA+TGG | + | chr3.1:67126758-67126777 | None:intergenic | 45.0% |
| !!! | ACAAATAAATATATTTTTAT+AGG | - | chr3.1:67126441-67126460 | MS.gene70510:intron | 5.0% |
| AAAGAGCAACTCCGTGCCAT+GGG | - | chr3.1:67126883-67126902 | MS.gene70510:intron | 50.0% | |
| ! | GAGTTGATCTCGCTTTCCCA+TGG | + | chr3.1:67126902-67126921 | None:intergenic | 50.0% |
| CAAAGAGCAACTCCGTGCCA+TGG | - | chr3.1:67126882-67126901 | MS.gene70510:intron | 55.0% | |
| GATCTCGCTTTCCCATGGCA+CGG | + | chr3.1:67126897-67126916 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 67126363 | 67127376 | 67126363 | ID=MS.gene70510 |
| chr3.1 | mRNA | 67126363 | 67127376 | 67126363 | ID=MS.gene70510.t1;Parent=MS.gene70510 |
| chr3.1 | exon | 67127320 | 67127376 | 67127320 | ID=MS.gene70510.t1.exon1;Parent=MS.gene70510.t1 |
| chr3.1 | CDS | 67127320 | 67127376 | 67127320 | ID=cds.MS.gene70510.t1;Parent=MS.gene70510.t1 |
| chr3.1 | exon | 67127000 | 67127092 | 67127000 | ID=MS.gene70510.t1.exon2;Parent=MS.gene70510.t1 |
| chr3.1 | CDS | 67127000 | 67127092 | 67127000 | ID=cds.MS.gene70510.t1;Parent=MS.gene70510.t1 |
| chr3.1 | exon | 67126811 | 67126883 | 67126811 | ID=MS.gene70510.t1.exon3;Parent=MS.gene70510.t1 |
| chr3.1 | CDS | 67126811 | 67126883 | 67126811 | ID=cds.MS.gene70510.t1;Parent=MS.gene70510.t1 |
| chr3.1 | exon | 67126363 | 67126382 | 67126363 | ID=MS.gene70510.t1.exon4;Parent=MS.gene70510.t1 |
| chr3.1 | CDS | 67126363 | 67126382 | 67126363 | ID=cds.MS.gene70510.t1;Parent=MS.gene70510.t1 |
| Gene Sequence |
| Protein sequence |
