Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70512.t1 | XP_024634747.1 | 97.1 | 518 | 15 | 0 | 5 | 522 | 17 | 534 | 3.20E-272 | 947.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70512.t1 | Q9SFD5 | 47.7 | 537 | 247 | 10 | 5 | 519 | 20 | 544 | 4.7e-123 | 443.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70512.t1 | A0A072V1B9 | 93.7 | 525 | 18 | 2 | 1 | 522 | 1 | 513 | 1.0e-259 | 905.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70512.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70512.t1 | MTR_3g081360 | 94.286 | 525 | 15 | 2 | 1 | 522 | 1 | 513 | 0.0 | 1009 |
| MS.gene70512.t1 | MTR_3g082790 | 80.479 | 543 | 80 | 4 | 5 | 521 | 21 | 563 | 0.0 | 866 |
| MS.gene70512.t1 | MTR_3g082790 | 80.688 | 523 | 76 | 3 | 24 | 521 | 1 | 523 | 0.0 | 840 |
| MS.gene70512.t1 | MTR_2g026310 | 50.471 | 531 | 246 | 6 | 5 | 519 | 19 | 548 | 1.74e-174 | 503 |
| MS.gene70512.t1 | MTR_7g063290 | 46.990 | 515 | 225 | 7 | 4 | 518 | 17 | 483 | 6.19e-147 | 431 |
| MS.gene70512.t1 | MTR_2g026380 | 45.402 | 522 | 233 | 8 | 4 | 519 | 17 | 492 | 9.18e-142 | 417 |
| MS.gene70512.t1 | MTR_3g081380 | 76.471 | 170 | 38 | 1 | 3 | 170 | 14 | 183 | 1.99e-92 | 280 |
| MS.gene70512.t1 | MTR_3g081390 | 53.416 | 161 | 33 | 2 | 5 | 165 | 7 | 125 | 1.86e-41 | 145 |
| MS.gene70512.t1 | MTR_3g081420 | 38.462 | 208 | 119 | 2 | 318 | 519 | 2 | 206 | 4.59e-41 | 147 |
| MS.gene70512.t1 | MTR_7g062030 | 58.442 | 77 | 32 | 0 | 6 | 82 | 33 | 109 | 3.21e-24 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70512.t1 | AT3G07740 | 51.397 | 537 | 227 | 10 | 5 | 519 | 20 | 544 | 6.50e-171 | 494 |
| MS.gene70512.t1 | AT4G16420 | 50.096 | 519 | 203 | 8 | 5 | 521 | 18 | 482 | 1.90e-170 | 490 |
| MS.gene70512.t1 | AT3G07740 | 51.737 | 518 | 217 | 9 | 24 | 519 | 17 | 523 | 2.16e-168 | 487 |
| MS.gene70512.t1 | AT3G07740 | 50.735 | 544 | 227 | 11 | 5 | 519 | 20 | 551 | 2.61e-168 | 488 |
| MS.gene70512.t1 | AT4G16420 | 49.713 | 523 | 203 | 9 | 5 | 521 | 18 | 486 | 3.09e-168 | 485 |
| MS.gene70512.t1 | AT4G16420 | 48.948 | 523 | 206 | 8 | 5 | 521 | 18 | 485 | 5.77e-167 | 482 |
| MS.gene70512.t1 | AT3G07740 | 51.760 | 483 | 203 | 8 | 56 | 519 | 2 | 473 | 2.92e-155 | 451 |
Find 90 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTAACTCCATCATTATTT+TGG | 0.150007 | 3.1:+67155786 | None:intergenic |
| TTTGGCAGAAATGGAATTTA+AGG | 0.163949 | 3.1:-67154381 | MS.gene70512:CDS |
| GTAGGATTTGTCTTATTCTA+TGG | 0.220086 | 3.1:-67157102 | MS.gene70512:intron |
| CAAAAGAATAAAAGACTTAA+AGG | 0.230732 | 3.1:-67153788 | MS.gene70512:intron |
| TAGATATGCATCAGATTTCT+TGG | 0.239365 | 3.1:+67152647 | None:intergenic |
| GAAAAGAAGCCTAAATTATC+TGG | 0.247074 | 3.1:-67154494 | MS.gene70512:CDS |
| TATGATATGCTTGTGGAAAA+AGG | 0.255246 | 3.1:-67152588 | MS.gene70512:CDS |
| GCTATTGACATGCATGGATT+TGG | 0.276427 | 3.1:-67157311 | MS.gene70512:CDS |
| GTTCACATTGGAAGCCTTCT+TGG | 0.280186 | 3.1:+67155808 | None:intergenic |
| TGCATAGAGTGCTTCTTTGT+TGG | 0.282569 | 3.1:-67159393 | MS.gene70512:CDS |
| TCACATATTTGAAGCTCTTC+AGG | 0.296633 | 3.1:+67153895 | None:intergenic |
| ACATGCATGGATTTGGGAAT+TGG | 0.309773 | 3.1:-67157304 | MS.gene70512:CDS |
| CAATGATGCTGAGCAAGTTT+TGG | 0.319202 | 3.1:-67154399 | MS.gene70512:CDS |
| TTTAATACCATAACTCGATA+TGG | 0.332731 | 3.1:+67159339 | None:intergenic |
| TTATATTCCCCAGATAATTT+AGG | 0.358245 | 3.1:+67154485 | None:intergenic |
| CCAAAATAATGATGGAGTTA+AGG | 0.371137 | 3.1:-67155786 | MS.gene70512:CDS |
| CTATTGACATGCATGGATTT+GGG | 0.372854 | 3.1:-67157310 | MS.gene70512:CDS |
| TCTTCACCTGCACTCCAATC+TGG | 0.373150 | 3.1:+67157483 | None:intergenic |
| TGTCTTTGTGATAGCTTGTA+TGG | 0.382689 | 3.1:+67152989 | None:intergenic |
| AGAGTCAGCTTTGCATTTGA+AGG | 0.391333 | 3.1:+67156119 | None:intergenic |
| TTGCAGGAAGCTAGAATTGC+TGG | 0.400705 | 3.1:-67153155 | MS.gene70512:intron |
| TATGGCTGGCGGTGCATCCT+TGG | 0.406764 | 3.1:+67153007 | None:intergenic |
| GGTGAGAGAAATTTCTCAAA+AGG | 0.408187 | 3.1:+67153916 | None:intergenic |
| ATCAATTTATTGAACAAAAG+AGG | 0.414539 | 3.1:-67153109 | MS.gene70512:CDS |
| TATAAAGAAAGTGGTCAGAT+TGG | 0.427378 | 3.1:-67153062 | MS.gene70512:CDS |
| GAAGCCTTCTTGGTTATACT+TGG | 0.427656 | 3.1:+67155818 | None:intergenic |
| TAAATTCTGTCAATCTTGCT+TGG | 0.438413 | 3.1:+67152609 | None:intergenic |
| CACAAAGACACTAGAAGAAT+GGG | 0.442742 | 3.1:-67152976 | MS.gene70512:CDS |
| CCTCTCAACACTTCAATAGA+TGG | 0.448042 | 3.1:+67154461 | None:intergenic |
| TAGGATTTGTCTTATTCTAT+GGG | 0.455897 | 3.1:-67157101 | MS.gene70512:CDS |
| TGAAGAGAAGTTACTTATTG+AGG | 0.462048 | 3.1:-67157466 | MS.gene70512:intron |
| CTTTAGGCTATTGACATGCA+TGG | 0.463532 | 3.1:-67157317 | MS.gene70512:intron |
| GAACTCCTTGCCATGGCAAA+AGG | 0.466099 | 3.1:-67157065 | MS.gene70512:CDS |
| TCACAAAGACACTAGAAGAA+TGG | 0.467289 | 3.1:-67152977 | MS.gene70512:CDS |
| AGAAATTTCTCAAAAGGATC+GGG | 0.467873 | 3.1:+67153922 | None:intergenic |
| TCAGATATACAGCATTATAA+TGG | 0.471582 | 3.1:+67157231 | None:intergenic |
| TTCCGCTTACATCTCCAGAT+TGG | 0.472380 | 3.1:-67157497 | MS.gene70512:CDS |
| AATGATGGAGTTAAGGTTGA+AGG | 0.474514 | 3.1:-67155779 | MS.gene70512:intron |
| TCTCAAGCAGGTTGGAGCAC+TGG | 0.475857 | 3.1:-67154518 | MS.gene70512:CDS |
| CCGCGTAATGTTGAGGAAGA+TGG | 0.476953 | 3.1:+67152704 | None:intergenic |
| AAAAGAAGCCTAAATTATCT+GGG | 0.481337 | 3.1:-67154493 | MS.gene70512:CDS |
| CAATCATCCATATCGAGTTA+TGG | 0.484056 | 3.1:-67159346 | MS.gene70512:intron |
| GGCGGTGCATCCTTGGTACT+AGG | 0.496097 | 3.1:+67153014 | None:intergenic |
| TTTACTTTATTCCATCAGAA+AGG | 0.497295 | 3.1:+67156084 | None:intergenic |
| AAAGAACCTCACCTTTCTGA+TGG | 0.498619 | 3.1:-67156095 | MS.gene70512:CDS |
| GAGAAATTTCTCAAAAGGAT+CGG | 0.500929 | 3.1:+67153921 | None:intergenic |
| GATTTAGAAGCTTCATATGC+AGG | 0.502552 | 3.1:-67159605 | MS.gene70512:intron |
| TTTGTGATAGCTTGTATGGC+TGG | 0.504362 | 3.1:+67152993 | None:intergenic |
| AATGTGAACCAAAATAATGA+TGG | 0.504833 | 3.1:-67155794 | MS.gene70512:CDS |
| CGCGTAATGTTGAGGAAGAT+GGG | 0.509064 | 3.1:+67152705 | None:intergenic |
| ACATCTCCAGATTGGAGTGC+AGG | 0.509957 | 3.1:-67157489 | MS.gene70512:CDS |
| TCAAATAGCCTTACAGGTAG+AGG | 0.513546 | 3.1:+67157194 | None:intergenic |
| TCAAATATGTGAACAGTACA+AGG | 0.524557 | 3.1:-67153884 | MS.gene70512:CDS |
| CATGCAGTTAGAAATTTCAA+AGG | 0.524634 | 3.1:-67152679 | MS.gene70512:CDS |
| CTATAGATTGTACCTGAGGT+TGG | 0.533420 | 3.1:+67155941 | None:intergenic |
| ATGCAGTTAGAAATTTCAAA+GGG | 0.542710 | 3.1:-67152678 | MS.gene70512:CDS |
| CAGCTCTGAAGAATCTAAGA+AGG | 0.544590 | 3.1:-67155993 | MS.gene70512:CDS |
| CAAGGTAACTATAAAGAAAG+TGG | 0.546753 | 3.1:-67153071 | MS.gene70512:CDS |
| AAAGGACATCAACTCAAGAA+AGG | 0.549772 | 3.1:-67157047 | MS.gene70512:intron |
| CAGAATTTATGATATGCTTG+TGG | 0.553524 | 3.1:-67152595 | MS.gene70512:CDS |
| TCTTCCAAGTATAACCAAGA+AGG | 0.569053 | 3.1:-67155822 | MS.gene70512:CDS |
| ATGGTGTTGCTGAAAACGTT+GGG | 0.572743 | 3.1:-67157280 | MS.gene70512:CDS |
| AACATGTCAAGGCCAACCTC+AGG | 0.574063 | 3.1:-67155953 | MS.gene70512:intron |
| TTGTTAGTAGAATCTCAAGC+AGG | 0.574478 | 3.1:-67154530 | MS.gene70512:intron |
| AGTGGTCAGATTGGAACAAG+TGG | 0.587270 | 3.1:-67153053 | MS.gene70512:CDS |
| TAAATCATCAGCATTTAAAG+CGG | 0.588889 | 3.1:+67159622 | None:intergenic |
| CATCTATTGAAGTGTTGAGA+GGG | 0.593154 | 3.1:-67154460 | MS.gene70512:CDS |
| GCATGATCCGCGTAATGTTG+AGG | 0.597253 | 3.1:+67152697 | None:intergenic |
| CATGGATTTGGGAATTGGAA+TGG | 0.599035 | 3.1:-67157299 | MS.gene70512:CDS |
| TAGTAGAATCTCAAGCAGGT+TGG | 0.601506 | 3.1:-67154526 | MS.gene70512:intron |
| CCATCTATTGAAGTGTTGAG+AGG | 0.602727 | 3.1:-67154461 | MS.gene70512:CDS |
| CCACTAATCAAAACATGTCA+AGG | 0.604753 | 3.1:-67155964 | MS.gene70512:CDS |
| CTTACAGGTAGAGGAAAACA+TGG | 0.611599 | 3.1:+67157203 | None:intergenic |
| CCATCTTCCTCAACATTACG+CGG | 0.613654 | 3.1:-67152704 | MS.gene70512:CDS |
| TGAACGTGAAATGAAACTGC+AGG | 0.617265 | 3.1:-67154342 | MS.gene70512:CDS |
| CAAAGAGGAACTCCTTGCCA+TGG | 0.618565 | 3.1:-67157072 | MS.gene70512:CDS |
| AGAATCATTTCCTAGTACCA+AGG | 0.625963 | 3.1:-67153024 | MS.gene70512:CDS |
| TTTATTCCATCAGAAAGGTG+AGG | 0.628847 | 3.1:+67156089 | None:intergenic |
| AGGACAAAAGAAGCTGAACA+AGG | 0.637958 | 3.1:-67153089 | MS.gene70512:CDS |
| TCACTCAAAAGAAGAGCATG+AGG | 0.651691 | 3.1:-67153848 | MS.gene70512:CDS |
| TTCTATGGGAAAGAACAAAG+AGG | 0.651784 | 3.1:-67157087 | MS.gene70512:CDS |
| GTGATAGCTTGTATGGCTGG+CGG | 0.658411 | 3.1:+67152996 | None:intergenic |
| AATGGTGTTGCTGAAAACGT+TGG | 0.659458 | 3.1:-67157281 | MS.gene70512:CDS |
| AACAGTACAAGGTCTTCATG+CGG | 0.668099 | 3.1:-67153873 | MS.gene70512:CDS |
| CTCCAATCTGGAGATGTAAG+CGG | 0.681010 | 3.1:+67157495 | None:intergenic |
| AAAGAAGCCTAAATTATCTG+GGG | 0.686550 | 3.1:-67154492 | MS.gene70512:CDS |
| AGAGGGTACAATTCTAACAG+AGG | 0.689534 | 3.1:-67154443 | MS.gene70512:CDS |
| GTAGGCATGGGAATAACTGA+TGG | 0.696306 | 3.1:-67159507 | MS.gene70512:intron |
| ATGAAACTGCAGGTGCTCCG+TGG | 0.711759 | 3.1:-67154332 | MS.gene70512:CDS |
| GTAATGTTGAGGAAGATGGG+TGG | 0.746783 | 3.1:+67152708 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 67152572 | 67159915 | 67152572 | ID=MS.gene70512 |
| chr3.1 | mRNA | 67152572 | 67159915 | 67152572 | ID=MS.gene70512.t1;Parent=MS.gene70512 |
| chr3.1 | exon | 67159905 | 67159915 | 67159905 | ID=MS.gene70512.t1.exon1;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67159905 | 67159915 | 67159905 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67159606 | 67159661 | 67159606 | ID=MS.gene70512.t1.exon2;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67159606 | 67159661 | 67159606 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67159347 | 67159525 | 67159347 | ID=MS.gene70512.t1.exon3;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67159347 | 67159525 | 67159347 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67157467 | 67157532 | 67157467 | ID=MS.gene70512.t1.exon4;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67157467 | 67157532 | 67157467 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67157208 | 67157333 | 67157208 | ID=MS.gene70512.t1.exon5;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67157208 | 67157333 | 67157208 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67157048 | 67157120 | 67157048 | ID=MS.gene70512.t1.exon6;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67157048 | 67157120 | 67157048 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67156089 | 67156146 | 67156089 | ID=MS.gene70512.t1.exon7;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67156089 | 67156146 | 67156089 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67155954 | 67156012 | 67155954 | ID=MS.gene70512.t1.exon8;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67155954 | 67156012 | 67155954 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67155780 | 67155857 | 67155780 | ID=MS.gene70512.t1.exon9;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67155780 | 67155857 | 67155780 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67154320 | 67154542 | 67154320 | ID=MS.gene70512.t1.exon10;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67154320 | 67154542 | 67154320 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67153789 | 67153999 | 67153789 | ID=MS.gene70512.t1.exon11;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67153789 | 67153999 | 67153789 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67152935 | 67153171 | 67152935 | ID=MS.gene70512.t1.exon12;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67152935 | 67153171 | 67152935 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| chr3.1 | exon | 67152572 | 67152763 | 67152572 | ID=MS.gene70512.t1.exon13;Parent=MS.gene70512.t1 |
| chr3.1 | CDS | 67152572 | 67152763 | 67152572 | ID=cds.MS.gene70512.t1;Parent=MS.gene70512.t1 |
| Gene Sequence |
| Protein sequence |
