AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70737

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70737.t1 MTR_3g080100 97.688 173 4 0 1 173 1 173 8.87e-120 338
MS.gene70737.t1 MTR_3g080100 97.761 134 3 0 40 173 1 134 3.46e-90 262
MS.gene70737.t1 MTR_8g089895 52.258 155 69 2 1 155 1 150 2.40e-50 164
MS.gene70737.t1 MTR_3g086790 54.140 157 69 3 1 155 1 156 1.00e-47 157
MS.gene70737.t1 MTR_8g468210 57.724 123 51 1 46 168 64 185 3.67e-38 132
MS.gene70737.t1 MTR_5g038280 66.327 98 33 0 59 156 87 184 5.38e-38 132
MS.gene70737.t1 MTR_5g039000 57.600 125 52 1 44 168 62 185 7.38e-38 131
MS.gene70737.t1 MTR_6g011610 61.261 111 40 1 42 152 63 170 1.24e-37 130
MS.gene70737.t1 MTR_6g011610 61.261 111 40 1 42 152 82 189 1.30e-37 130
MS.gene70737.t1 MTR_8g469430 61.538 104 40 0 59 162 89 192 5.24e-36 126
MS.gene70737.t1 MTR_4g107650 63.810 105 38 0 58 162 94 198 6.34e-36 127
MS.gene70737.t1 MTR_6g007647 61.682 107 41 0 58 164 32 138 4.39e-35 123
MS.gene70737.t1 MTR_6g007647 61.682 107 41 0 58 164 66 172 1.12e-34 123
MS.gene70737.t1 MTR_7g010020 57.407 108 43 1 47 154 71 175 2.95e-33 119
MS.gene70737.t1 MTR_7g103340 51.376 109 53 0 52 160 56 164 2.80e-29 108
MS.gene70737.t1 MTR_1g061660 48.649 111 57 0 58 168 58 168 2.05e-25 97.8
MS.gene70737.t1 MTR_3g092150 49.451 91 46 0 61 151 40 130 9.28e-24 94.0
MS.gene70737.t1 MTR_8g026960 48.837 86 44 0 66 151 41 126 2.02e-23 92.8
MS.gene70737.t1 MTR_4g100550 46.667 105 49 1 49 153 110 207 4.30e-20 85.1
MS.gene70737.t1 MTR_5g019680 43.220 118 55 1 51 168 45 150 1.46e-19 82.0
MS.gene70737.t1 MTR_4g097600 45.946 111 51 2 43 153 113 214 1.77e-19 83.2
MS.gene70737.t1 MTR_4g126900 46.739 92 42 1 62 153 91 175 9.29e-19 80.1
MS.gene70737.t1 MTR_5g019650 42.636 129 62 4 51 174 45 166 3.69e-18 78.6
MS.gene70737.t1 MTR_1g054285 46.226 106 48 2 48 153 145 241 4.83e-17 77.0
MS.gene70737.t1 MTR_5g013010 48.421 95 42 1 59 153 136 223 4.93e-17 77.0
MS.gene70737.t1 MTR_7g093430 45.283 106 49 2 48 153 151 247 5.24e-17 76.6
MS.gene70737.t1 MTR_2g061030 47.368 95 43 1 59 153 200 287 9.55e-17 76.6
MS.gene70737.t1 MTR_5g014890 47.368 95 43 1 59 153 123 210 6.87e-16 73.6
MS.gene70737.t1 MTR_1g017090 46.316 95 44 1 59 153 130 217 1.20e-15 73.2
MS.gene70737.t1 MTR_8g006705 47.872 94 45 1 59 152 146 235 2.53e-15 72.4
MS.gene70737.t1 MTR_4g084750 44.211 95 46 1 59 153 65 152 2.94e-14 67.8
MS.gene70737.t1 MTR_3g103590 47.297 74 39 0 57 130 143 216 1.62e-13 67.4
MS.gene70737.t1 MTR_2g038625 40.566 106 55 2 48 153 72 169 9.55e-13 63.9
MS.gene70737.t1 MTR_2g038580 40.566 106 55 2 48 153 72 169 9.55e-13 63.9
MS.gene70737.t1 MTR_5g013010 57.692 52 22 0 59 110 136 187 2.18e-11 60.5
MS.gene70737.t1 MTR_5g029320 35.106 94 61 0 45 138 78 171 5.83e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70737.t1 AT5G65310 53.571 168 65 4 1 155 1 168 1.54e-50 165
MS.gene70737.t1 AT5G65310 59.441 143 47 2 24 155 8 150 1.59e-49 162
MS.gene70737.t1 AT4G40060 47.531 162 71 3 1 155 1 155 4.29e-44 147
MS.gene70737.t1 AT2G22430 49.686 159 74 2 1 155 2 158 1.55e-43 147
MS.gene70737.t1 AT1G69780 60.163 123 48 1 46 168 72 193 9.75e-40 136
MS.gene70737.t1 AT5G15150 58.140 129 50 3 42 167 95 222 6.06e-38 132
MS.gene70737.t1 AT1G26960 51.034 145 65 2 28 172 45 183 1.69e-36 127
MS.gene70737.t1 AT3G01220 56.637 113 47 1 44 154 70 182 2.07e-34 122
MS.gene70737.t1 AT3G01470 60.000 100 40 0 56 155 65 164 4.93e-33 119
MS.gene70737.t1 AT2G46680 50.538 93 46 0 60 152 33 125 1.44e-26 101
MS.gene70737.t1 AT1G27045 53.535 99 46 0 57 155 15 113 2.48e-26 99.0
MS.gene70737.t1 AT1G27045 53.535 99 46 0 57 155 23 121 2.69e-26 99.0
MS.gene70737.t1 AT1G27045 53.535 99 46 0 57 155 23 121 2.69e-26 99.0
MS.gene70737.t1 AT1G27045 53.535 99 46 0 57 155 66 164 4.35e-26 99.8
MS.gene70737.t1 AT2G46680 48.958 96 48 1 60 154 33 128 3.15e-25 98.2
MS.gene70737.t1 AT3G61890 44.444 108 57 1 62 169 33 137 4.24e-25 97.4
MS.gene70737.t1 AT1G27045 52.632 95 45 0 57 151 66 160 1.03e-24 96.7
MS.gene70737.t1 AT5G03790 39.370 127 70 1 50 169 68 194 2.26e-21 87.4
MS.gene70737.t1 AT2G18550 46.491 114 56 1 59 172 61 169 2.90e-21 87.0
MS.gene70737.t1 AT4G36740 46.903 113 56 1 52 160 48 160 3.27e-21 86.7
MS.gene70737.t1 AT4G36740 46.903 113 56 1 52 160 49 161 3.41e-21 86.7
MS.gene70737.t1 AT5G03790 47.253 91 48 0 50 140 68 158 9.71e-21 84.7
MS.gene70737.t1 AT2G36610 46.000 100 46 1 59 150 71 170 5.75e-20 82.8
MS.gene70737.t1 AT5G66700 43.636 110 60 1 46 155 60 167 6.84e-20 83.6
MS.gene70737.t1 AT5G53980 41.237 97 57 0 59 155 11 107 1.85e-18 78.2
MS.gene70737.t1 AT2G01430 43.119 109 55 1 45 153 125 226 2.77e-18 80.1
MS.gene70737.t1 AT5G06710 40.146 137 75 3 17 152 149 279 4.23e-17 77.4
MS.gene70737.t1 AT4G17460 41.270 126 63 3 32 153 104 222 7.84e-17 76.3
MS.gene70737.t1 AT5G47370 41.538 130 63 2 23 152 103 219 1.45e-16 75.5
MS.gene70737.t1 AT2G44910 46.316 95 44 1 59 153 163 250 6.59e-16 73.9
MS.gene70737.t1 AT4G37790 42.241 116 59 2 38 153 106 213 1.14e-15 73.2
MS.gene70737.t1 AT4G16780 44.660 103 49 2 51 153 122 216 2.19e-15 72.4
MS.gene70737.t1 AT3G60390 42.056 107 55 1 47 153 150 249 2.46e-15 72.4
MS.gene70737.t1 AT2G22800 41.176 119 68 2 51 169 106 222 1.05e-14 70.5
MS.gene70737.t1 AT1G70920 37.600 125 76 1 20 144 2 124 4.85e-13 64.3
MS.gene70737.t1 AT2G01430 43.529 85 44 1 45 125 125 209 5.48e-13 64.7
MS.gene70737.t1 AT1G70920 36.719 128 79 1 17 144 29 154 7.25e-13 64.3
MS.gene70737.t1 AT5G06710 38.298 94 57 1 17 110 149 241 1.28e-11 61.6

Find 22 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTTCGTGCTCGACGATTT+TGG 0.160811 3.1:-68197348 None:intergenic
CTTTCTATACCTCTTGAAAT+TGG 0.202435 3.1:-68197648 None:intergenic
GTGAAGTTAGCTGAAGATTT+AGG 0.236761 3.1:+68197295 MS.gene70737:CDS
AACCTTAAGAACATCAAAAT+TGG 0.244715 3.1:-68197410 None:intergenic
AACCTAGACAAGTTTCAATT+TGG 0.262579 3.1:+68197323 MS.gene70737:CDS
ATAATCTCTTTCCAATTGTT+TGG 0.328373 3.1:-68197374 None:intergenic
TTTAGAAAAGAGCTTTGAAT+TGG 0.355867 3.1:+68197246 MS.gene70737:CDS
GTTCAATAACTCGGAAACTA+TGG 0.361291 3.1:+68196958 MS.gene70737:CDS
TTCTATATATACCTTTGGAT+TGG 0.453479 3.1:-68197000 None:intergenic
TATACCTTTGGATTGGTACA+TGG 0.467749 3.1:-68196993 None:intergenic
ACGAACGAAAACCAAACAAT+TGG 0.484787 3.1:+68197363 MS.gene70737:CDS
GAATGAGACTCTTACTAGAA+AGG 0.510168 3.1:+68197456 MS.gene70737:CDS
CAAGAAGATAGCTATGAAGA+TGG 0.532967 3.1:+68197172 MS.gene70737:CDS
TGAAGATGGTAGTTCAATGT+TGG 0.535009 3.1:+68197186 MS.gene70737:CDS
TGCAAATGCAGAGGTGGATG+TGG 0.541032 3.1:+68197087 MS.gene70737:intron
AACCAAATTGAAACTTGTCT+AGG 0.545141 3.1:-68197325 None:intergenic
GATCAGAGGAATGAGACAAA+AGG 0.552396 3.1:+68197109 MS.gene70737:CDS
TTGGAGAAGAAAAGAAGACT+TGG 0.569876 3.1:+68197205 MS.gene70737:CDS
CTTTCCATGTACCAATCCAA+AGG 0.573223 3.1:+68196989 MS.gene70737:CDS
GTAGAATGTCCAATTTCAAG+AGG 0.608484 3.1:+68197639 MS.gene70737:CDS
AATGAAGAGGTTCAATAACT+CGG 0.609369 3.1:+68196949 None:intergenic
CAGAGGTGGATGTGGATCAG+AGG 0.615698 3.1:+68197095 MS.gene70737:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACACAAAATAAAATTAAAA+AGG - chr3.1:68197036-68197055 None:intergenic 15.0%
!! ATTACTTCTATATATACCTT+TGG - chr3.1:68197008-68197027 None:intergenic 20.0%
!!! TTATTTTGTGTGTAATTTTG+TGG + chr3.1:68197044-68197063 MS.gene70737:intron 20.0%
! AAAGAACCATTGAAACATAT+AGG - chr3.1:68197732-68197751 None:intergenic 25.0%
! AACCTTAAGAACATCAAAAT+TGG - chr3.1:68197413-68197432 None:intergenic 25.0%
! ATAATCTCTTTCCAATTGTT+TGG - chr3.1:68197377-68197396 None:intergenic 25.0%
! TAATTCAAGTGGTGTAATTA+AGG + chr3.1:68197686-68197705 MS.gene70737:intron 25.0%
! TTCTATATATACCTTTGGAT+TGG - chr3.1:68197003-68197022 None:intergenic 25.0%
!!! TTTAGAAAAGAGCTTTGAAT+TGG + chr3.1:68197246-68197265 MS.gene70737:CDS 25.0%
AACCAAATTGAAACTTGTCT+AGG - chr3.1:68197328-68197347 None:intergenic 30.0%
AACCTAGACAAGTTTCAATT+TGG + chr3.1:68197323-68197342 MS.gene70737:CDS 30.0%
CTTTCTATACCTCTTGAAAT+TGG - chr3.1:68197651-68197670 None:intergenic 30.0%
GATTCTCCTATATGTTTCAA+TGG + chr3.1:68197723-68197742 MS.gene70737:intron 30.0%
! ATTTCAAGAGGTATAGAAAG+AGG + chr3.1:68197651-68197670 MS.gene70737:intron 30.0%
!! GTCCAATTTTGATGTTCTTA+AGG + chr3.1:68197408-68197427 MS.gene70737:CDS 30.0%
ACGAACGAAAACCAAACAAT+TGG + chr3.1:68197363-68197382 MS.gene70737:CDS 35.0%
CAAGAAGATAGCTATGAAGA+TGG + chr3.1:68197172-68197191 MS.gene70737:CDS 35.0%
GAATGAGACTCTTACTAGAA+AGG + chr3.1:68197456-68197475 MS.gene70737:CDS 35.0%
GGTTCAGAAAGTAATTCAAG+TGG + chr3.1:68197675-68197694 MS.gene70737:intron 35.0%
GTAGAATGTCCAATTTCAAG+AGG + chr3.1:68197639-68197658 MS.gene70737:CDS 35.0%
GTGAAGTTAGCTGAAGATTT+AGG + chr3.1:68197295-68197314 MS.gene70737:CDS 35.0%
GTTCAATAACTCGGAAACTA+TGG + chr3.1:68196958-68196977 MS.gene70737:CDS 35.0%
TGAAGATGGTAGTTCAATGT+TGG + chr3.1:68197186-68197205 MS.gene70737:CDS 35.0%
TTCATTCTTTGCAAATGCAG+AGG + chr3.1:68197078-68197097 MS.gene70737:intron 35.0%
TTGGAGAAGAAAAGAAGACT+TGG + chr3.1:68197205-68197224 MS.gene70737:CDS 35.0%
!! TATACCTTTGGATTGGTACA+TGG - chr3.1:68196996-68197015 None:intergenic 35.0%
ATTCTTTGCAAATGCAGAGG+TGG + chr3.1:68197081-68197100 MS.gene70737:intron 40.0%
CTTTCCATGTACCAATCCAA+AGG + chr3.1:68196989-68197008 MS.gene70737:CDS 40.0%
GATCAGAGGAATGAGACAAA+AGG + chr3.1:68197109-68197128 MS.gene70737:CDS 40.0%
! TCAAGAGGTATAGAAAGAGG+AGG + chr3.1:68197654-68197673 MS.gene70737:intron 40.0%
!! GCTAACTTCACTTTTCTCTC+TGG - chr3.1:68197286-68197305 None:intergenic 40.0%
TGCAAATGCAGAGGTGGATG+TGG + chr3.1:68197087-68197106 MS.gene70737:intron 50.0%
! TCGTTCGTGCTCGACGATTT+TGG - chr3.1:68197351-68197370 None:intergenic 50.0%
CAGAGGTGGATGTGGATCAG+AGG + chr3.1:68197095-68197114 MS.gene70737:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 68196950 68197765 68196950 ID=MS.gene70737
chr3.1 mRNA 68196950 68197765 68196950 ID=MS.gene70737.t1;Parent=MS.gene70737
chr3.1 exon 68196950 68197010 68196950 ID=MS.gene70737.t1.exon1;Parent=MS.gene70737.t1
chr3.1 CDS 68196950 68197010 68196950 ID=cds.MS.gene70737.t1;Parent=MS.gene70737.t1
chr3.1 exon 68197098 68197477 68197098 ID=MS.gene70737.t1.exon2;Parent=MS.gene70737.t1
chr3.1 CDS 68197098 68197477 68197098 ID=cds.MS.gene70737.t1;Parent=MS.gene70737.t1
chr3.1 exon 68197582 68197660 68197582 ID=MS.gene70737.t1.exon3;Parent=MS.gene70737.t1
chr3.1 CDS 68197582 68197660 68197582 ID=cds.MS.gene70737.t1;Parent=MS.gene70737.t1
chr3.1 exon 68197758 68197765 68197758 ID=MS.gene70737.t1.exon4;Parent=MS.gene70737.t1
chr3.1 CDS 68197758 68197765 68197758 ID=cds.MS.gene70737.t1;Parent=MS.gene70737.t1
Gene Sequence

>MS.gene70737

ATGAAGAGGTTCAATAACTCGGAAACTATGGCTTCTTTACTTTCCATGTACCAATCCAAAGGTATATATAGAAGTAATTCAAACCTTTTTAATTTTATTTTGTGTGTAATTTTGTGGTCTAATTATGTTTCATTCTTTGCAAATGCAGAGGTGGATGTGGATCAGAGGAATGAGACAAAAGGTTACAGTGAAGAATTTCAAGCAATGTTAGATAGATTAGAGCAAGAAGATAGCTATGAAGATGGTAGTTCAATGTTGGAGAAGAAAAGAAGACTTGGTTATGATCAAGTAAAAGCTTTAGAAAAGAGCTTTGAATTGGACAACAAGCTAGAGCCAGAGAGAAAAGTGAAGTTAGCTGAAGATTTAGGCTTGCAACCTAGACAAGTTTCAATTTGGTTCCAAAATCGTCGAGCACGAACGAAAACCAAACAATTGGAAAGAGATTATAGTGTTCTTAAGTCCAATTTTGATGTTCTTAAGGTTGAGTATAGTAATCTTCAACAAGAGAATGAGACTCTTACTAGAAAGGTAATATATTACATATAGTATGTATTATTCATGATTTTTTTGTTTATGTGAATGATGTTAATGTGTAATCTTTGTGTCTTTTTGTGTGTGTGTGTGAACTGCAGTTAAGAGAGTTGAAAGCGAAATTGTTAAGAGTTGAAAGCGATGAGTTAGAGAAAGAAGTAGAATGTCCAATTTCAAGAGGTATAGAAAGAGGAGGTTCAGAAAGTAATTCAAGTGGTGTAATTAAGGAAGAAAGTAATGTTGATTCTCCTATATGTTTCAATGGTTCTTTGCCAAGTTCAATGA
Protein sequence

>MS.gene70737.t1

MKRFNNSETMASLLSMYQSKEVDVDQRNETKGYSEEFQAMLDRLEQEDSYEDGSSMLEKKRRLGYDQVKALEKSFELDNKLEPERKVKLAEDLGLQPRQVSIWFQNRRARTKTKQLERDYSVLKSNFDVLKVEYSNLQQENETLTRKLRELKAKLLRVESDELEKEVECPISRVQ