AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71193.t1 MTR_8g066820 99.574 235 1 0 1 235 1 235 1.39e-179 492
MS.gene71193.t1 MTR_5g030130 84.874 238 32 3 1 235 1 237 3.75e-148 412
MS.gene71193.t1 MTR_5g030130 87.952 166 18 1 1 164 1 166 7.18e-109 311
MS.gene71193.t1 MTR_5g007230 69.767 215 56 3 19 233 7 212 2.61e-107 308
MS.gene71193.t1 MTR_4g078170 72.059 204 53 4 31 233 13 213 6.65e-104 300
MS.gene71193.t1 MTR_1g062160 55.340 206 73 5 33 229 7 202 7.59e-73 220
MS.gene71193.t1 MTR_7g102790 58.333 204 71 5 33 229 7 203 2.36e-71 217
MS.gene71193.t1 MTR_2g060540 49.630 135 58 4 34 158 11 145 1.10e-38 132
MS.gene71193.t1 MTR_2g060500 48.148 135 60 3 34 158 11 145 2.13e-38 131
MS.gene71193.t1 MTR_4g116300 48.031 127 65 1 33 159 6 131 7.03e-37 130
MS.gene71193.t1 MTR_2g055210 31.818 264 130 6 9 228 82 339 3.64e-36 130
MS.gene71193.t1 MTR_2g084495 34.052 232 109 6 34 228 5 229 3.75e-36 127
MS.gene71193.t1 MTR_4g094680 33.478 230 118 7 26 228 15 236 4.82e-35 125
MS.gene71193.t1 MTR_2g084525 46.715 137 63 4 34 160 5 141 8.17e-32 117
MS.gene71193.t1 MTR_2g084510 43.066 137 68 3 34 160 4 140 1.21e-30 114
MS.gene71193.t1 MTR_2g036170 31.532 222 119 6 33 228 37 251 9.38e-28 106
MS.gene71193.t1 MTR_8g105930 33.951 162 82 6 19 159 1 158 1.62e-16 76.3
MS.gene71193.t1 MTR_8g097310 29.193 161 99 4 72 228 4 153 2.98e-15 70.9
MS.gene71193.t1 MTR_5g077740 26.866 201 105 8 33 228 23 186 7.40e-15 70.9
MS.gene71193.t1 MTR_1g032880 33.929 112 60 4 79 180 27 134 5.91e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71193.t1 AT4G17900 73.391 233 52 5 1 229 1 227 4.38e-122 346
MS.gene71193.t1 AT4G17900 72.906 203 46 4 30 229 17 213 1.09e-103 299
MS.gene71193.t1 AT1G32700 70.968 217 53 5 24 235 2 213 2.07e-103 298
MS.gene71193.t1 AT1G32700 70.391 179 44 4 61 235 1 174 5.66e-80 238
MS.gene71193.t1 AT5G46710 52.521 238 90 8 1 228 1 225 8.95e-78 234
MS.gene71193.t1 AT1G21000 50.209 239 82 4 26 228 6 243 6.62e-77 232
MS.gene71193.t1 AT1G21000 50.209 239 82 4 26 228 9 246 8.50e-77 232
MS.gene71193.t1 AT1G76590 50.877 228 80 4 33 228 18 245 2.02e-74 226
MS.gene71193.t1 AT5G46710 52.830 212 84 6 26 228 5 209 4.40e-72 219
MS.gene71193.t1 AT5G46710 59.627 161 57 3 1 159 1 155 1.38e-66 203
MS.gene71193.t1 AT1G43000 46.083 217 101 4 26 228 2 216 4.21e-62 194
MS.gene71193.t1 AT1G43000 46.083 217 101 4 26 228 9 223 4.55e-62 194
MS.gene71193.t1 AT2G27930 44.712 208 83 5 33 230 4 189 3.77e-53 170
MS.gene71193.t1 AT2G27930 44.712 208 83 5 33 230 4 189 3.77e-53 170
MS.gene71193.t1 AT2G27930 55.474 137 51 2 33 159 4 140 8.90e-49 158
MS.gene71193.t1 AT2G27930 55.474 137 51 2 33 159 4 140 8.90e-49 158
MS.gene71193.t1 AT2G12646 48.819 127 64 1 33 159 6 131 2.73e-37 131
MS.gene71193.t1 AT2G12646 48.819 127 64 1 33 159 6 131 2.73e-37 131
MS.gene71193.t1 AT2G12646 48.819 127 64 1 33 159 3 128 2.81e-37 131
MS.gene71193.t1 AT3G60670 46.212 132 70 1 28 159 2 132 9.30e-36 127
MS.gene71193.t1 AT1G31040 34.513 226 116 5 33 228 19 242 2.72e-34 123
MS.gene71193.t1 AT2G27930 45.455 154 53 4 86 230 61 192 5.39e-34 121
MS.gene71193.t1 AT2G27930 45.455 154 53 4 86 230 61 192 5.39e-34 121
MS.gene71193.t1 AT2G01818 34.579 214 115 9 35 228 13 221 4.13e-22 90.9
MS.gene71193.t1 AT2G01818 33.987 153 81 7 92 228 9 157 9.98e-11 58.9

Find 49 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTCCCTTTCTTCTTT+TGG 0.157849 8.3:-36236336 None:intergenic
CAAAGATCATCGTGCTATTC+AGG 0.214126 8.3:+36234792 MS.gene71193:CDS
GAGCTGACTTGTTCTTCTTC+TGG 0.262316 8.3:-36236187 None:intergenic
CCTTGCACTGTTGATAATAT+AGG 0.282719 8.3:-36235384 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8.3:+36235318 MS.gene71193:CDS
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8.3:+36235494 MS.gene71193:CDS
AACTATGAATTCTAGATTGT+TGG 0.377091 8.3:+36236149 MS.gene71193:intron
TTCATACCATGATGTGATTA+GGG 0.385029 8.3:+36235319 MS.gene71193:CDS
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8.3:+36235353 MS.gene71193:CDS
ACTGTTGGTGGTGGTGTTGA+AGG 0.411165 8.3:-36236299 None:intergenic
TATTGTTTGGATTGTATGAA+TGG 0.420599 8.3:+36234739 MS.gene71193:CDS
TGAGTGCAATATGTATTGTT+TGG 0.429253 8.3:+36234726 MS.gene71193:CDS
TGGAAGACTGAAGAACATTA+AGG 0.432710 8.3:+36236262 MS.gene71193:CDS
TGTGTTTGATACAGATAAGA+AGG 0.447190 8.3:+36235294 MS.gene71193:intron
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8.3:+36234626 MS.gene71193:CDS
GCAGCACTAGTCCTCCCATC+GGG 0.455937 8.3:-36236367 None:intergenic
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8.3:+36236242 MS.gene71193:CDS
TAATGTTCTTCAGTCTTCCA+TGG 0.466799 8.3:-36236259 None:intergenic
CAGATCAGCCAAAAGAAGAA+AGG 0.467780 8.3:+36236328 MS.gene71193:CDS
GAATAGCACGATGATCTTTG+TGG 0.487973 8.3:-36234789 None:intergenic
GCTGATCTGTAATTGACTGT+TGG 0.490334 8.3:-36236314 None:intergenic
CCTCAGCCAAGGCCCGGAAA+AGG 0.495211 8.3:+36235428 MS.gene71193:CDS
AGATCAGCCAAAAGAAGAAA+GGG 0.506864 8.3:+36236329 MS.gene71193:CDS
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8.3:+36235502 MS.gene71193:CDS
CCTATATTATCAACAGTGCA+AGG 0.522886 8.3:+36235384 MS.gene71193:CDS
GAAAGGCCTCAGCCAAGGCC+CGG 0.523082 8.3:+36235422 MS.gene71193:CDS
GATCTGTAATTGACTGTTGG+TGG 0.533120 8.3:-36236311 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.534739 8.3:-36236308 None:intergenic
GGGTTGTGTTCTTGAATGAA+AGG 0.538393 8.3:+36235405 MS.gene71193:CDS
TGCAGCACTAGTCCTCCCAT+CGG 0.541315 8.3:-36236368 None:intergenic
ATTCCGCATCGTGCCCCGAT+GGG 0.553697 8.3:+36236353 MS.gene71193:CDS
AATTCCGCATCGTGCCCCGA+TGG 0.556752 8.3:+36236352 MS.gene71193:CDS
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8.3:-36235456 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8.3:-36235325 None:intergenic
CCGCATCGTGCCCCGATGGG+AGG 0.573029 8.3:+36236356 MS.gene71193:CDS
TTGAGCAGAGGTTTCAACCA+TGG 0.583253 8.3:-36234655 None:intergenic
AAGAAACGCTCTTTGAGCAG+AGG 0.583635 8.3:-36234667 None:intergenic
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8.3:+36235343 MS.gene71193:CDS
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8.3:+36234638 MS.gene71193:CDS
TGAATGAAAGGCCTCAGCCA+AGG 0.624070 8.3:+36235417 MS.gene71193:CDS
CCTCCCATCGGGGCACGATG+CGG 0.625934 8.3:-36236356 None:intergenic
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8.3:-36235495 None:intergenic
AGCAGAGGTTTCAACCATGG+TGG 0.633067 8.3:-36234652 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8.3:-36235484 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8.3:+36234618 MS.gene71193:CDS
TTCAATGATATCAGACTCAG+AGG 0.648076 8.3:+36236214 MS.gene71193:CDS
CTATATTATCAACAGTGCAA+GGG 0.657853 8.3:+36235385 MS.gene71193:CDS
CTGATGATGAGGAAAACAGG+TGG 0.717367 8.3:+36234629 MS.gene71193:CDS
CAGCACTAGTCCTCCCATCG+GGG 0.730442 8.3:-36236366 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTTATAAAAAAATT+GGG + chr8.3:36234348-36234367 MS.gene71193:intron 0.0%
!!! AAAATTTGATTTTTTTTACA+CGG + chr8.3:36234110-36234129 MS.gene71193:intron 10.0%
!!! ATTATTATTACTACTATTTT+GGG - chr8.3:36235587-36235606 None:intergenic 10.0%
!!! TAATTATTTTTAAGTTAACT+TGG + chr8.3:36234480-36234499 MS.gene71193:intron 10.0%
!!! TTAATTTTTTGTTTTGTTTT+TGG + chr8.3:36234202-36234221 MS.gene71193:intron 10.0%
!!! CTATTTAAAATATTGATCAA+TGG + chr8.3:36235869-36235888 MS.gene71193:intron 15.0%
!!! GATTATTATTACTACTATTT+TGG - chr8.3:36235588-36235607 None:intergenic 15.0%
!!! TATTTGTGTTTTTAAATTCT+GGG + chr8.3:36234245-36234264 MS.gene71193:intron 15.0%
!!! TTATTTGTGTTTTTAAATTC+TGG + chr8.3:36234244-36234263 MS.gene71193:intron 15.0%
!!! TTGATCAATATTTTAAATAG+TGG - chr8.3:36235869-36235888 None:intergenic 15.0%
!!! TTTGATAATTTGTTTTTGAA+GGG + chr8.3:36234595-36234614 MS.gene71193:intron 15.0%
!!! TTTTAGAGAGAAAAAAAATT+AGG + chr8.3:36234517-36234536 MS.gene71193:intron 15.0%
!!! TTTTATAAAAAAATTGACAC+TGG - chr8.3:36234343-36234362 None:intergenic 15.0%
!!! TTTTGATAATTTGTTTTTGA+AGG + chr8.3:36234594-36234613 MS.gene71193:intron 15.0%
!! AAATAATAAACACACAATGA+AGG - chr8.3:36234158-36234177 None:intergenic 20.0%
!! AAATTGAATTGAATGCAAAA+GGG - chr8.3:36236055-36236074 None:intergenic 20.0%
!! AATAATTGAATTGATTGTAG+TGG - chr8.3:36235128-36235147 None:intergenic 20.0%
!! ATAATCATGAATGCTAATAT+AGG + chr8.3:36235963-36235982 MS.gene71193:intron 20.0%
!! TAAATTGAATTGAATGCAAA+AGG - chr8.3:36236056-36236075 None:intergenic 20.0%
!! TATTATAGACTATTCTTATG+AGG - chr8.3:36235774-36235793 None:intergenic 20.0%
!! TTGAAATGACAAAATTTCAA+AGG - chr8.3:36236026-36236045 None:intergenic 20.0%
!!! ATTTGTTTTGATTGCAATTT+TGG + chr8.3:36235692-36235711 MS.gene71193:intron 20.0%
!!! GAAATTTTGTCATTTCAATA+TGG + chr8.3:36236028-36236047 MS.gene71193:intron 20.0%
!!! TATTTGTGATTTTGAATTGT+GGG + chr8.3:36234560-36234579 MS.gene71193:intron 20.0%
!!! TCTTGTATTTTAGAGAATTA+CGG - chr8.3:36235808-36235827 None:intergenic 20.0%
!!! TGTTTGTGTTTTTGAATTTT+GGG + chr8.3:36234079-36234098 MS.gene71193:intron 20.0%
!!! TTATTTGTGATTTTGAATTG+TGG + chr8.3:36234559-36234578 MS.gene71193:intron 20.0%
!!! TTGTTTGTGTTTTTGAATTT+TGG + chr8.3:36234078-36234097 MS.gene71193:intron 20.0%
!!! TTTTGGAATGTAATGTTTTT+GGG + chr8.3:36234219-36234238 MS.gene71193:intron 20.0%
!!! TTTTTGGAATGTAATGTTTT+TGG + chr8.3:36234218-36234237 MS.gene71193:intron 20.0%
! AAAAACAGAGTTCATTAGAA+AGG - chr8.3:36235272-36235291 None:intergenic 25.0%
! AACACAATAAATGTGTCAAA+GGG - chr8.3:36234894-36234913 None:intergenic 25.0%
! AACTATGAATTCTAGATTGT+TGG + chr8.3:36236149-36236168 MS.gene71193:intron 25.0%
! AATGACAAAATTTCAAAGGT+TGG - chr8.3:36236022-36236041 None:intergenic 25.0%
! AATGGGAATTTATGGATAAT+TGG - chr8.3:36234992-36235011 None:intergenic 25.0%
! ATGACAAAATTTCAAAGGTT+GGG - chr8.3:36236021-36236040 None:intergenic 25.0%
! GAACAATAAATGGGAATTTA+TGG - chr8.3:36235000-36235019 None:intergenic 25.0%
! TAGAAATTGAAAACAGCAAA+AGG - chr8.3:36234964-36234983 None:intergenic 25.0%
! TATTGTTTGGATTGTATGAA+TGG + chr8.3:36234739-36234758 MS.gene71193:CDS 25.0%
!!! TAACTTTTGAGTTTATGCAT+TGG + chr8.3:36236112-36236131 MS.gene71193:intron 25.0%
AAATTTCAAAGGTTGGGTAT+AGG - chr8.3:36236015-36236034 None:intergenic 30.0%
ATCTTGTTCAAATGGTTAGA+AGG - chr8.3:36235559-36235578 None:intergenic 30.0%
CACAACTTTGATGAATTCAA+TGG - chr8.3:36234835-36234854 None:intergenic 30.0%
CTATATTATCAACAGTGCAA+GGG + chr8.3:36235385-36235404 MS.gene71193:CDS 30.0%
GAACACAATAAATGTGTCAA+AGG - chr8.3:36234895-36234914 None:intergenic 30.0%
TCTTGTTCAAATGGTTAGAA+GGG - chr8.3:36235558-36235577 None:intergenic 30.0%
TGAGTGCAATATGTATTGTT+TGG + chr8.3:36234726-36234745 MS.gene71193:CDS 30.0%
TGATTTAACGACCAAAAGTT+TGG - chr8.3:36235616-36235635 None:intergenic 30.0%
TGTGTTTGATACAGATAAGA+AGG + chr8.3:36235294-36235313 MS.gene71193:intron 30.0%
TTCATACCATGATGTGATTA+GGG + chr8.3:36235319-36235338 MS.gene71193:CDS 30.0%
! AACTTTTGGTCGTTAAATCA+AGG + chr8.3:36235616-36235635 MS.gene71193:intron 30.0%
! AGCTTTTCAATGCAATAACA+TGG + chr8.3:36235241-36235260 MS.gene71193:intron 30.0%
! ATTGGGAATGCAATGATTTT+AGG + chr8.3:36234365-36234384 MS.gene71193:intron 30.0%
! GTTTTTCTGACAACAAAAAC+AGG - chr8.3:36234917-36234936 None:intergenic 30.0%
!! AAAAACCATCACTTTTCCTT+TGG + chr8.3:36234931-36234950 MS.gene71193:intron 30.0%
!!! TGTTTTTGGGCTTAATTTCA+CGG - chr8.3:36233908-36233927 None:intergenic 30.0%
ACCTCAATGCATTAACATTG+AGG - chr8.3:36235730-36235749 None:intergenic 35.0%
ACCTCAATGTTAATGCATTG+AGG + chr8.3:36235726-36235745 MS.gene71193:intron 35.0%
AGATCAGCCAAAAGAAGAAA+GGG + chr8.3:36236329-36236348 MS.gene71193:CDS 35.0%
CAAATCCAAAGGAAAAGTGA+TGG - chr8.3:36234939-36234958 None:intergenic 35.0%
CCTATATTATCAACAGTGCA+AGG + chr8.3:36235384-36235403 MS.gene71193:CDS 35.0%
CTTCATACCATGATGTGATT+AGG + chr8.3:36235318-36235337 MS.gene71193:CDS 35.0%
CTTGTTCAAATGGTTAGAAG+GGG - chr8.3:36235557-36235576 None:intergenic 35.0%
GGGAGTTAATCTTGTTCAAA+TGG - chr8.3:36235567-36235586 None:intergenic 35.0%
GGTGGTTACGAACAATAAAT+GGG - chr8.3:36235009-36235028 None:intergenic 35.0%
GTGAAATTAAGCCCAAAAAC+AGG + chr8.3:36233907-36233926 MS.gene71193:CDS 35.0%
TAATGTTCTTCAGTCTTCCA+TGG - chr8.3:36236262-36236281 None:intergenic 35.0%
TCAAGCTAGCACTTAAAAGT+GGG + chr8.3:36235928-36235947 MS.gene71193:intron 35.0%
TGGAAGACTGAAGAACATTA+AGG + chr8.3:36236262-36236281 MS.gene71193:CDS 35.0%
TGGTGGTTACGAACAATAAA+TGG - chr8.3:36235010-36235029 None:intergenic 35.0%
TTCAAGCTAGCACTTAAAAG+TGG + chr8.3:36235927-36235946 MS.gene71193:intron 35.0%
TTCAATGATATCAGACTCAG+AGG + chr8.3:36236214-36236233 MS.gene71193:CDS 35.0%
TTGACACCAACATCAAATGT+CGG - chr8.3:36235092-36235111 None:intergenic 35.0%
TTTAACGACCAAAAGTTTGG+TGG - chr8.3:36235613-36235632 None:intergenic 35.0%
! ATAATCCACCACCAAACTTT+TGG + chr8.3:36235602-36235621 MS.gene71193:intron 35.0%
! ATTAGTGTCATTGTTGTGTC+TGG + chr8.3:36235176-36235195 MS.gene71193:intron 35.0%
! GAATGAGATTCAGAAAGTGT+TGG + chr8.3:36235343-36235362 MS.gene71193:CDS 35.0%
! TACCATGATTCTCCTGTTTT+TGG - chr8.3:36233922-36233941 None:intergenic 35.0%
!! ACCATGATTCTCCTGTTTTT+GGG - chr8.3:36233921-36233940 None:intergenic 35.0%
!! CATCACTTTTCCTTTGGATT+TGG + chr8.3:36234937-36234956 MS.gene71193:intron 35.0%
!! CCTTGCACTGTTGATAATAT+AGG - chr8.3:36235387-36235406 None:intergenic 35.0%
!!! AATTTGTTTTTGAAGGGTGC+TGG + chr8.3:36234601-36234620 MS.gene71193:intron 35.0%
AAGGGGAAGACATTATGTGA+AGG - chr8.3:36235540-36235559 None:intergenic 40.0%
AGAATCGAAAGGAATCGAGA+AGG - chr8.3:36235487-36235506 None:intergenic 40.0%
AGCAAAAGGACCAAATCCAA+AGG - chr8.3:36234950-36234969 None:intergenic 40.0%
AGGTTGGGTATAGGAACAAA+TGG - chr8.3:36236006-36236025 None:intergenic 40.0%
CAGATCAGCCAAAAGAAGAA+AGG + chr8.3:36236328-36236347 MS.gene71193:CDS 40.0%
CATTCACCCTAATCACATCA+TGG - chr8.3:36235328-36235347 None:intergenic 40.0%
GAATAGCACGATGATCTTTG+TGG - chr8.3:36234792-36234811 None:intergenic 40.0%
GATCTGTAATTGACTGTTGG+TGG - chr8.3:36236314-36236333 None:intergenic 40.0%
GCTGATCTGTAATTGACTGT+TGG - chr8.3:36236317-36236336 None:intergenic 40.0%
GGGTTGTGTTCTTGAATGAA+AGG + chr8.3:36235405-36235424 MS.gene71193:CDS 40.0%
TCCTTTCGATTCTGTTCTCT+TGG + chr8.3:36235494-36235513 MS.gene71193:CDS 40.0%
TTAGCTCACACTCTCATTGT+TGG + chr8.3:36233988-36234007 MS.gene71193:intron 40.0%
TTGATTGTAGTGGCATACAC+CGG - chr8.3:36235118-36235137 None:intergenic 40.0%
! CAAAGATCATCGTGCTATTC+AGG + chr8.3:36234792-36234811 MS.gene71193:CDS 40.0%
! CGGAATTCCCTTTCTTCTTT+TGG - chr8.3:36236339-36236358 None:intergenic 40.0%
!! CAGAAAGTGTTGGACATAAC+AGG + chr8.3:36235353-36235372 MS.gene71193:CDS 40.0%
!! TTGATGTTGGTGTCAAATGC+CGG + chr8.3:36235096-36235115 MS.gene71193:intron 40.0%
AAGAAACGCTCTTTGAGCAG+AGG - chr8.3:36234670-36234689 None:intergenic 45.0%
ATTCTGTTCTCTTGGCTGCA+AGG + chr8.3:36235502-36235521 MS.gene71193:CDS 45.0%
CAATCTAAACCATCGATGCG+TGG - chr8.3:36235030-36235049 None:intergenic 45.0%
CAGACACCGACATTTGATGT+TGG + chr8.3:36235083-36235102 MS.gene71193:intron 45.0%
GCCAAGAGAACAGAATCGAA+AGG - chr8.3:36235498-36235517 None:intergenic 45.0%
GCCCAAAAACAGGAGAATCA+TGG + chr8.3:36233917-36233936 MS.gene71193:CDS 45.0%
TGTCTGACACCAACATGTGT+CGG - chr8.3:36235069-36235088 None:intergenic 45.0%
TTGAGCAGAGGTTTCAACCA+TGG - chr8.3:36234658-36234677 None:intergenic 45.0%
! AACGACCAAAAGTTTGGTGG+TGG - chr8.3:36235610-36235629 None:intergenic 45.0%
! CACCTGTTTTCCTCATCATC+AGG - chr8.3:36234631-36234650 None:intergenic 45.0%
! CTGATGATGAGGAAAACAGG+TGG + chr8.3:36234629-36234648 MS.gene71193:CDS 45.0%
! CTGTAATTGACTGTTGGTGG+TGG - chr8.3:36236311-36236330 None:intergenic 45.0%
! GTCCTGATGATGAGGAAAAC+AGG + chr8.3:36234626-36234645 MS.gene71193:CDS 45.0%
!! GAGCTGACTTGTTCTTCTTC+TGG - chr8.3:36236190-36236209 None:intergenic 45.0%
!!! AGTGTTGGTGACACCTTTTC+CGG - chr8.3:36235444-36235463 None:intergenic 45.0%
!!! TTGTTTTTGAAGGGTGCTGG+AGG + chr8.3:36234604-36234623 MS.gene71193:intron 45.0%
!!! CAATTTTTTTATAAAAAAAT+TGG + chr8.3:36234347-36234366 MS.gene71193:intron 5.0%
AGCAGAGGTTTCAACCATGG+TGG - chr8.3:36234655-36234674 None:intergenic 50.0%
CTCGCAGACTTCACAAGTGT+TGG - chr8.3:36235459-36235478 None:intergenic 50.0%
GTTCACTGTCCGACACATGT+TGG + chr8.3:36235057-36235076 MS.gene71193:intron 50.0%
TCTAAACCATCGATGCGTGG+TGG - chr8.3:36235027-36235046 None:intergenic 50.0%
TGAATGAAAGGCCTCAGCCA+AGG + chr8.3:36235417-36235436 MS.gene71193:CDS 50.0%
!! ACTGTTGGTGGTGGTGTTGA+AGG - chr8.3:36236302-36236321 None:intergenic 50.0%
!!! GTGTTGGTGACACCTTTTCC+GGG - chr8.3:36235443-36235462 None:intergenic 50.0%
AGGAAAACAGGTGGCCACCA+TGG + chr8.3:36234638-36234657 MS.gene71193:CDS 55.0%
TCGTAACCACCACGCATCGA+TGG + chr8.3:36235018-36235037 MS.gene71193:intron 55.0%
TGCAGCACTAGTCCTCCCAT+CGG - chr8.3:36236371-36236390 None:intergenic 55.0%
! TGCTGGAGGTCCTGATGATG+AGG + chr8.3:36234618-36234637 MS.gene71193:CDS 55.0%
AATTCCGCATCGTGCCCCGA+TGG + chr8.3:36236352-36236371 MS.gene71193:CDS 60.0%
ATTCCGCATCGTGCCCCGAT+GGG + chr8.3:36236353-36236372 MS.gene71193:CDS 60.0%
CACAGCAGCAGTTGCAGCCA+TGG + chr8.3:36236242-36236261 MS.gene71193:CDS 60.0%
CAGCACTAGTCCTCCCATCG+GGG - chr8.3:36236369-36236388 None:intergenic 60.0%
GCAGCACTAGTCCTCCCATC+GGG - chr8.3:36236370-36236389 None:intergenic 60.0%
! GTGACACCTTTTCCGGGCCT+TGG - chr8.3:36235437-36235456 None:intergenic 60.0%
CCTCAGCCAAGGCCCGGAAA+AGG + chr8.3:36235428-36235447 MS.gene71193:CDS 65.0%
GAAAGGCCTCAGCCAAGGCC+CGG + chr8.3:36235422-36235441 MS.gene71193:CDS 65.0%
! CCTTTTCCGGGCCTTGGCTG+AGG - chr8.3:36235431-36235450 None:intergenic 65.0%
CCTCCCATCGGGGCACGATG+CGG - chr8.3:36236359-36236378 None:intergenic 70.0%
CCGCATCGTGCCCCGATGGG+AGG + chr8.3:36236356-36236375 MS.gene71193:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 36233879 36236397 36233879 ID=MS.gene71193
chr8.3 mRNA 36233879 36236397 36233879 ID=MS.gene71193.t1;Parent=MS.gene71193
chr8.3 exon 36233879 36233938 36233879 ID=MS.gene71193.t1.exon1;Parent=MS.gene71193.t1
chr8.3 CDS 36233879 36233938 36233879 ID=cds.MS.gene71193.t1;Parent=MS.gene71193.t1
chr8.3 exon 36234616 36234813 36234616 ID=MS.gene71193.t1.exon2;Parent=MS.gene71193.t1
chr8.3 CDS 36234616 36234813 36234616 ID=cds.MS.gene71193.t1;Parent=MS.gene71193.t1
chr8.3 exon 36235308 36235523 36235308 ID=MS.gene71193.t1.exon3;Parent=MS.gene71193.t1
chr8.3 CDS 36235308 36235523 36235308 ID=cds.MS.gene71193.t1;Parent=MS.gene71193.t1
chr8.3 exon 36236164 36236397 36236164 ID=MS.gene71193.t1.exon4;Parent=MS.gene71193.t1
chr8.3 CDS 36236164 36236397 36236164 ID=cds.MS.gene71193.t1;Parent=MS.gene71193.t1
Gene Sequence

>MS.gene71193

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGGTAAATTAAAACTCTTTCTTCAATTTTCTATTACATGTTTTGTTTTCATTTAGCTCACACTCTCATTGTTGGTTCATTATTTGTGAATCGACTTTGATAAACTAGTGTTAATTTTTTGAAAATGCAATGATTTTGTGTATTGTTTGTGTTTTTGAATTTTGGGTCTGATTCAAAATTTGATTTTTTTTACACGGTTTTATTTTTATTTAGTTCAGTCCTTCATTGTGTGTTTATTATTTGTGATTTGACTTGAAGAATTAGTGTTAATTTTTTGTTTTGTTTTTGGAATGTAATGTTTTTGGGTTTTATTTGTGTTTTTAAATTCTGGGTCTGATTCAAAATTTGATTTTTTCTTTTTTTACATATTTTATTTAGTGTAGATTCTCATTGTGTGTTCATTACCAGTGTCAATTTTTTTATAAAAAAATTGGGAATGCAATGATTTTAGGTATTATTTGTGTTTTTGAATAATGAGTCTGATTCAAATTGTAATTTTTTTACACAGTTTTATTTTTATTTAGCTCAAACTCTCATTGTGTGTTAATTATTTTTAAGTTAACTTGGAAAACTAGTGTTAATTTTAGAGAGAAAAAAAATTAGGAATGTGATGATTTTGTGTATTATTTGTGATTTTGAATTGTGGGTCTGATTCAAATTTTGATAATTTGTTTTTGAAGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTGCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGTGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGGTTTGTTAGTTGTTACAACCATTGAATTCATCAAAGTTGTGTGATTTCAACTGTTGTTTGTAGTTGTTGTTCTTTTACCCTTTGACACATTTATTGTGTTCCTGTTTTTGTTGTCAGAAAAACCATCACTTTTCCTTTGGATTTGGTCCTTTTGCTGTTTTCAATTTCTACACAACCAATTATCCATAAATTCCCATTTATTGTTCGTAACCACCACGCATCGATGGTTTAGATTGAATGTGTGTTCACTGTCCGACACATGTTGGTGTCAGACACCGACATTTGATGTTGGTGTCAAATGCCGGTGTATGCCACTACAATCAATTCAATTATTTTTTTAAAATTATTATTGATGACAATGAATTAGTGTCATTGTTGTGTCTGGTGTCTGTGTCTAGTTCAGTGTCGCAGAAGTGAAAAATGTTTTAGCTTTTCAATGCAATAACATGGTTATTCCTTTCTAATGAACTCTGTTTTTTGTGTGTTTGATACAGATAAGAAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAAAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGGTATTTTTACATTCCTTCACATAATGTCTTCCCCTTCTAACCATTTGAACAAGATTAACTCCCAAAATAGTAGTAATAATAATCCACCACCAAACTTTTGGTCGTTAAATCAAGGATTTTGATGTTTCAATTTGTTAATGATAATATGATTGAAGTTGCATTGATCACATTTGTTTTGATTGCAATTTTGGATATTATCAAGACCTCAATGTTAATGCATTGAGGTCACATCAACTACATTATTAGTCCTCATAAGAATAGTCTATAATAGTTGCATTTGACCGTAATTCTCTAAAATACAAGACTCAACATCATTATTATTATTTTTATTTTTTTATCAATCCACTATTTAAAATATTGATCAATGGTATCTCTTTTTTAAGAAAAAACTATTTGCTTTATTTTCAAGCTAGCACTTAAAAGTGGGTTGACTTTGCTTATAATCATGAATGCTAATATAGGTTTATTTTTAACTTTTTCCATTTGTTCCTATACCCAACCTTTGAAATTTTGTCATTTCAATATGGACCCTTTTGCATTCAATTCAATTTAATTTTATTGACTTGTCAAGATAATTTATTCTTGTTTTAACTTTTGAGTTTATGCATTGGATACTAATCTATGCAACTATGAATTCTAGATTGTTGGAACTTCAATAAATTTCCAGAAGAAGAACAAGTCAGCTCGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTGAAGAACATTAAGGTGCAAAGTTTCACTCCTTCAACACCACCACCAACAGTCAATTACAGATCAGCCAAAAGAAGAAAGGGAATTCCGCATCGTGCCCCGATGGGAGGACTAGTGCTGCAATACTAA
Protein sequence

>MS.gene71193.t1

MAFENQDTVREIKPKNRRIMGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSINFQKKNKSARASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVLQY