AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71245

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71245.t1 MTR_8g065740 96.795 468 14 1 1 468 1 467 0.0 928
MS.gene71245.t1 MTR_5g048860 57.495 527 148 13 1 460 1 518 2.36e-175 503
MS.gene71245.t1 MTR_1g017350 42.540 315 141 10 115 421 92 374 1.94e-55 190
MS.gene71245.t1 MTR_1g017350 42.540 315 141 10 115 421 92 374 2.14e-55 190
MS.gene71245.t1 MTR_1g017350 41.590 327 135 12 115 416 92 387 7.29e-53 183
MS.gene71245.t1 MTR_1g017350 41.590 327 135 12 115 416 92 387 1.18e-52 183
MS.gene71245.t1 MTR_7g053410 39.459 370 184 13 126 468 128 484 2.23e-52 184
MS.gene71245.t1 MTR_7g053410 39.459 370 184 13 126 468 128 484 2.74e-52 185
MS.gene71245.t1 MTR_3g116770 44.361 266 111 8 135 393 43 278 1.27e-51 179
MS.gene71245.t1 MTR_3g116770 44.361 266 111 8 135 393 43 278 1.42e-51 179
MS.gene71245.t1 MTR_8g012290 39.053 338 159 15 127 431 93 416 1.06e-48 174
MS.gene71245.t1 MTR_8g012290 39.053 338 159 15 127 431 93 416 1.08e-48 174
MS.gene71245.t1 MTR_4g070320 71.963 107 28 1 289 393 124 230 1.05e-45 162
MS.gene71245.t1 MTR_8g062240 50.588 170 64 3 240 395 77 240 1.69e-41 149
MS.gene71245.t1 MTR_3g498825 85.366 82 12 0 297 378 138 219 1.16e-40 149
MS.gene71245.t1 MTR_5g037250 63.115 122 43 2 284 405 91 210 1.71e-40 145
MS.gene71245.t1 MTR_8g099880 82.353 85 14 1 302 385 141 225 3.86e-40 147
MS.gene71245.t1 MTR_7g092510 73.034 89 24 0 303 391 170 258 2.57e-39 145
MS.gene71245.t1 MTR_1g059270 68.085 94 30 0 303 396 157 250 8.11e-39 141
MS.gene71245.t1 MTR_1g059270 72.093 86 24 0 303 388 157 242 1.50e-37 140
MS.gene71245.t1 MTR_6g084120 52.252 111 42 2 277 385 124 225 2.93e-26 107
MS.gene71245.t1 MTR_4g131160 64.935 77 26 1 295 371 185 260 1.14e-21 97.1
MS.gene71245.t1 MTR_4g131160 64.935 77 26 1 295 371 188 263 1.24e-21 97.1
MS.gene71245.t1 MTR_1g080890 68.182 66 20 1 306 371 193 257 4.24e-20 92.0
MS.gene71245.t1 MTR_4g033295 55.385 65 29 0 306 370 118 182 2.23e-15 75.1
MS.gene71245.t1 MTR_4g065870 58.462 65 27 0 306 370 355 419 2.82e-15 78.2
MS.gene71245.t1 MTR_4g033295 53.731 67 30 1 306 371 118 184 3.95e-15 73.9
MS.gene71245.t1 MTR_2g098310 50.704 71 35 0 306 376 332 402 6.28e-15 77.0
MS.gene71245.t1 MTR_4g065870 58.065 62 26 0 306 367 355 416 1.42e-14 75.9
MS.gene71245.t1 MTR_2g098310 56.250 64 28 0 306 369 332 395 2.20e-14 75.1
MS.gene71245.t1 MTR_1g052470 52.174 69 33 0 306 374 298 366 3.04e-14 74.7
MS.gene71245.t1 MTR_7g092540 50.725 69 34 0 306 374 243 311 3.66e-14 73.6
MS.gene71245.t1 MTR_1g084980 33.125 160 82 5 244 402 415 550 4.38e-14 75.1
MS.gene71245.t1 MTR_1g069155 54.688 64 28 1 308 371 319 381 4.93e-14 74.7
MS.gene71245.t1 MTR_1g070870 63.636 66 23 1 306 371 120 184 1.41e-13 71.2
MS.gene71245.t1 MTR_1g070870 63.636 66 23 1 306 371 123 187 1.53e-13 71.2
MS.gene71245.t1 MTR_7g092540 50.769 65 32 0 306 370 243 307 1.88e-13 71.2
MS.gene71245.t1 MTR_3g028520 50.769 65 32 0 306 370 322 386 8.28e-13 70.5
MS.gene71245.t1 MTR_3g027650 50.769 65 32 0 306 370 322 386 8.28e-13 70.5
MS.gene71245.t1 MTR_7g099540 51.562 64 30 1 308 371 254 316 1.69e-12 69.7
MS.gene71245.t1 MTR_1g019240 43.210 81 40 2 292 372 76 150 2.07e-12 67.8
MS.gene71245.t1 MTR_3g027650 51.613 62 30 0 306 367 322 383 4.73e-12 67.8
MS.gene71245.t1 MTR_8g036235 47.692 65 34 0 306 370 316 380 8.11e-12 67.0
MS.gene71245.t1 MTR_1g018090 50.769 65 31 1 306 370 274 337 1.97e-11 65.9
MS.gene71245.t1 MTR_3g449770 50.000 64 31 1 308 371 311 373 4.07e-11 65.5
MS.gene71245.t1 MTR_7g099540 48.438 64 32 1 308 371 254 316 4.14e-11 65.5
MS.gene71245.t1 MTR_5g017040 53.571 56 25 1 317 372 116 170 6.06e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71245.t1 AT1G68920 44.493 454 197 15 17 455 28 441 1.02e-98 305
MS.gene71245.t1 AT1G68920 44.493 454 197 15 17 455 28 441 1.02e-98 305
MS.gene71245.t1 AT1G68920 44.493 454 197 15 17 455 28 441 1.02e-98 305
MS.gene71245.t1 AT1G68920 44.493 454 196 15 17 455 28 440 1.14e-98 305
MS.gene71245.t1 AT1G26260 40.189 423 165 14 25 441 13 353 8.20e-70 227
MS.gene71245.t1 AT1G26260 40.189 423 165 14 25 441 13 353 8.20e-70 227
MS.gene71245.t1 AT1G26260 40.189 423 165 14 25 441 13 353 8.20e-70 227
MS.gene71245.t1 AT1G26260 40.189 423 165 14 25 441 13 353 8.20e-70 227
MS.gene71245.t1 AT1G26260 46.006 313 122 9 135 441 60 331 6.78e-66 216
MS.gene71245.t1 AT1G26260 52.137 234 85 4 208 441 96 302 5.82e-64 210
MS.gene71245.t1 AT1G10120 52.083 192 82 2 214 405 124 305 4.68e-54 185
MS.gene71245.t1 AT1G10120 52.083 192 82 2 214 405 124 305 4.68e-54 185
MS.gene71245.t1 AT3G07340 41.791 268 101 7 127 393 132 345 6.83e-49 173
MS.gene71245.t1 AT3G07340 41.791 268 101 7 127 393 132 345 1.04e-48 173
MS.gene71245.t1 AT5G48560 40.149 269 132 7 127 393 148 389 1.90e-46 167
MS.gene71245.t1 AT5G48560 40.149 269 132 7 127 393 148 389 3.92e-46 167
MS.gene71245.t1 AT4G34530 68.750 112 30 2 303 409 169 280 8.31e-45 160
MS.gene71245.t1 AT3G23690 45.022 231 70 9 263 438 109 337 1.66e-44 160
MS.gene71245.t1 AT5G50915 56.410 156 49 6 261 414 106 244 2.78e-42 151
MS.gene71245.t1 AT5G50915 56.410 156 49 6 261 414 106 244 2.78e-42 151
MS.gene71245.t1 AT2G18300 54.110 146 62 1 240 385 124 264 3.65e-42 153
MS.gene71245.t1 AT2G18300 54.110 146 62 1 240 385 124 264 4.03e-42 152
MS.gene71245.t1 AT5G50915 51.667 180 62 8 261 437 106 263 4.09e-42 151
MS.gene71245.t1 AT5G50915 51.667 180 62 8 261 437 106 263 4.09e-42 151
MS.gene71245.t1 AT2G18300 54.110 146 62 1 240 385 124 264 7.26e-42 152
MS.gene71245.t1 AT1G59640 65.455 110 37 1 286 395 117 225 6.12e-41 147
MS.gene71245.t1 AT1G59640 42.424 198 108 3 197 393 31 223 2.02e-40 148
MS.gene71245.t1 AT4G36540 51.974 152 64 2 269 411 101 252 2.65e-40 147
MS.gene71245.t1 AT4G36540 59.200 125 48 1 269 390 101 225 3.61e-39 144
MS.gene71245.t1 AT2G42300 67.778 90 29 0 303 392 182 271 1.53e-38 141
MS.gene71245.t1 AT3G57800 73.256 86 23 0 303 388 201 286 1.65e-37 141
MS.gene71245.t1 AT2G42300 68.539 89 28 0 303 391 182 270 3.19e-37 139
MS.gene71245.t1 AT5G62610 74.074 81 20 1 297 377 145 224 1.01e-35 134
MS.gene71245.t1 AT5G62610 74.074 81 20 1 297 377 145 224 1.01e-35 134
MS.gene71245.t1 AT1G73830 61.321 106 41 0 280 385 121 226 2.24e-34 129
MS.gene71245.t1 AT1G18400 62.264 106 35 2 304 407 143 245 2.25e-34 129
MS.gene71245.t1 AT1G73830 61.321 106 41 0 280 385 121 226 2.39e-34 129
MS.gene71245.t1 AT1G18400 74.390 82 21 0 304 385 143 224 3.96e-34 129
MS.gene71245.t1 AT1G25330 52.713 129 55 2 270 394 66 192 4.96e-34 127
MS.gene71245.t1 AT3G57800 47.368 133 23 1 303 388 201 333 6.40e-29 118
MS.gene71245.t1 AT2G24260 59.091 88 33 2 284 371 15 99 6.28e-23 97.8
MS.gene71245.t1 AT4G30980 56.122 98 37 2 274 371 103 194 1.83e-22 98.2
MS.gene71245.t1 AT4G30980 56.122 98 37 2 274 371 151 242 3.70e-22 97.8
MS.gene71245.t1 AT2G24260 59.091 88 33 2 284 371 118 202 4.40e-22 97.8
MS.gene71245.t1 AT5G58010 70.149 67 19 1 305 371 98 163 3.85e-21 94.0
MS.gene71245.t1 AT2G42300 81.395 43 8 0 303 345 182 224 4.62e-17 80.5
MS.gene71245.t1 AT2G42300 81.395 43 8 0 303 345 182 224 4.62e-17 80.5
MS.gene71245.t1 AT2G42300 81.395 43 8 0 303 345 182 224 5.52e-17 80.5
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 241 342 4.96e-16 80.9
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 241 342 4.96e-16 80.9
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 241 342 4.96e-16 80.9
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 241 342 4.96e-16 80.9
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 170 271 5.03e-16 80.1
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 170 271 5.03e-16 80.1
MS.gene71245.t1 AT2G20180 40.909 110 57 3 262 371 170 271 5.03e-16 80.1
MS.gene71245.t1 AT2G20180 40.187 107 56 3 262 368 241 339 8.29e-15 75.9
MS.gene71245.t1 AT4G28790 33.562 146 82 4 275 416 236 370 1.06e-14 76.3
MS.gene71245.t1 AT1G51140 56.250 64 28 0 306 369 304 367 1.94e-14 75.1
MS.gene71245.t1 AT4G28800 34.959 123 60 3 268 389 229 332 5.68e-14 74.3
MS.gene71245.t1 AT1G35460 52.308 65 31 0 306 370 181 245 5.77e-14 72.4
MS.gene71245.t1 AT4G09180 51.515 66 32 0 305 370 183 248 7.18e-14 72.0
MS.gene71245.t1 AT2G42280 51.562 64 31 0 306 369 279 342 1.05e-13 72.8
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT1G09530 51.562 64 30 1 308 371 339 401 1.24e-13 73.6
MS.gene71245.t1 AT4G28815 56.897 58 24 1 309 366 71 127 2.18e-13 70.1
MS.gene71245.t1 AT2G42280 53.030 66 29 1 306 369 279 344 2.19e-13 72.0
MS.gene71245.t1 AT4G28800 34.375 128 59 3 268 389 241 349 3.33e-13 72.0
MS.gene71245.t1 AT4G02590 62.121 66 24 1 306 371 83 147 4.30e-13 69.3
MS.gene71245.t1 AT4G02590 62.121 66 24 1 306 371 146 210 6.11e-13 70.1
MS.gene71245.t1 AT4G02590 62.121 66 24 1 306 371 146 210 6.11e-13 70.1
MS.gene71245.t1 AT4G28815 56.897 58 24 1 309 366 147 203 6.57e-13 69.7
MS.gene71245.t1 AT1G03040 62.121 66 24 1 306 371 139 203 7.57e-13 69.7
MS.gene71245.t1 AT1G03040 62.121 66 24 1 306 371 144 208 9.05e-13 69.3
MS.gene71245.t1 AT1G03040 62.121 66 24 1 306 371 144 208 1.67e-12 68.9
MS.gene71245.t1 AT4G28790 34.951 103 62 2 275 373 236 337 3.23e-12 68.2
MS.gene71245.t1 AT4G00050 49.275 69 34 1 302 370 101 168 5.54e-12 67.0
MS.gene71245.t1 AT1G27740 37.398 123 60 4 264 370 109 230 5.77e-12 66.2
MS.gene71245.t1 AT2G46970 34.513 113 68 2 304 411 216 327 6.01e-12 67.8
MS.gene71245.t1 AT2G46970 34.513 113 68 2 304 411 185 296 6.04e-12 67.8
MS.gene71245.t1 AT2G46970 34.513 113 68 2 304 411 194 305 7.07e-12 67.4
MS.gene71245.t1 AT4G00050 49.275 69 34 1 302 370 203 270 1.25e-11 66.6
MS.gene71245.t1 AT4G00050 49.275 69 34 1 302 370 203 270 1.56e-11 66.2
MS.gene71245.t1 AT2G43140 50.000 64 32 0 306 369 231 294 3.38e-11 64.7
MS.gene71245.t1 AT2G43140 50.000 64 32 0 306 369 134 197 3.69e-11 63.2
MS.gene71245.t1 AT4G33880 49.231 65 32 1 306 370 266 329 4.54e-11 64.7
MS.gene71245.t1 AT4G36930 50.820 61 29 1 312 372 197 256 6.92e-11 64.3

Find 125 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATATGGTCCTAATTTCT+TGG 0.241465 8.3:-36929942 MS.gene71245:CDS
TATGAATCCACCATTGAATT+AGG 0.277485 8.3:+36929959 None:intergenic
TGCAATGGATTAACCTGATT+TGG 0.278397 8.3:+36927429 None:intergenic
AGTGGAGAACCTTCTATTAA+AGG 0.279819 8.3:-36929416 MS.gene71245:CDS
GACTAGAACCGTACATCTTT+AGG 0.302334 8.3:+36927726 None:intergenic
GCTTCAACAACCAAGGCTTC+TGG 0.303821 8.3:-36928800 MS.gene71245:CDS
CACTTGCACAATCCAACATT+TGG 0.316306 8.3:+36929435 None:intergenic
CCTTCATCTTGAGACATTAC+AGG 0.318475 8.3:+36929524 None:intergenic
GCTCTCACAGTTGTCCTTTG+TGG 0.319104 8.3:+36928847 None:intergenic
TGCAAATTCATCTGTAGGTT+TGG 0.339897 8.3:-36930020 MS.gene71245:CDS
ACCGGGTCCATCATCTGCTC+TGG 0.343461 8.3:-36927610 MS.gene71245:CDS
GGATTTAGACCTTTAATAGA+AGG 0.344206 8.3:+36929407 None:intergenic
ATGAGGGATACGTACATGTT+AGG 0.347532 8.3:-36928736 MS.gene71245:CDS
GTAAGAAGGTCCAAGAAATT+AGG 0.353442 8.3:+36929932 None:intergenic
GAAAGGAGGGTCCTTTAGAT+TGG 0.355470 8.3:-36930066 MS.gene71245:CDS
TCAACCACAGTTGGAACCTT+TGG 0.357269 8.3:-36927469 MS.gene71245:CDS
TCTGATAAATTTGCCATGTT+AGG 0.358693 8.3:+36927510 None:intergenic
ATGTGGGATTCCTCAATCTA+TGG 0.359558 8.3:-36929630 MS.gene71245:CDS
TTCTGGGAAGAATGCCAAAC+AGG 0.366913 8.3:-36928783 MS.gene71245:CDS
TCAGATTCTTCAACATCAAC+CGG 0.372154 8.3:-36927628 MS.gene71245:intron
TCTTACTAACTCTGAAAACT+TGG 0.374246 8.3:-36929915 MS.gene71245:CDS
TTCTTCATGTCCTAATTCAA+TGG 0.389007 8.3:-36929969 MS.gene71245:CDS
GGCATTCTTCCCAGAAGCCT+TGG 0.393219 8.3:+36928790 None:intergenic
ATCTGTAGGTTTGGTTTCCA+TGG 0.397148 8.3:-36930011 MS.gene71245:CDS
AGTCACTTCTTCGATCAATC+TGG 0.397941 8.3:+36929549 None:intergenic
CTTCAACAACCAAGGCTTCT+GGG 0.404138 8.3:-36928799 MS.gene71245:CDS
TTCCATGGAAAATTCTATGA+TGG 0.411759 8.3:-36929996 MS.gene71245:CDS
GGTTGATATGAATGTGATAA+TGG 0.412278 8.3:+36927555 None:intergenic
GTTGAGGAAATTTCAGTCCT+TGG 0.419505 8.3:+36927401 None:intergenic
CAGTCCTTGGCTGCTTGCAA+TGG 0.420935 8.3:+36927414 None:intergenic
AGCAATGAAGTGAGAAAAGA+TGG 0.430121 8.3:-36929866 MS.gene71245:CDS
CCAAAGAAAAGGAAAAGAAA+TGG 0.434507 8.3:-36929386 MS.gene71245:CDS
CCGGGTCCATCATCTGCTCT+GGG 0.439623 8.3:-36927609 MS.gene71245:CDS
AGAAGCTTGAGAACCCTGTT+TGG 0.448506 8.3:+36928769 None:intergenic
TTATTCTGTTTGTAGGTCAC+TGG 0.450051 8.3:-36928428 MS.gene71245:intron
TCTCGGTCTGTTGAGCATCT+TGG 0.450146 8.3:-36929590 MS.gene71245:CDS
GTTGGAATGGGTTGGGACCT+TGG 0.452583 8.3:-36929806 MS.gene71245:CDS
CTTCTTCGATCAATCTGGAT+TGG 0.454895 8.3:+36929554 None:intergenic
ACTTGCACAATCCAACATTT+GGG 0.456863 8.3:+36929436 None:intergenic
GGAAACATCTCAGCCCCATT+TGG 0.462856 8.3:+36929749 None:intergenic
TTTCTTCAAGATCTTGTACC+TGG 0.462998 8.3:-36928549 MS.gene71245:CDS
AGGGTTTAAAGAGCCAAATC+AGG 0.463980 8.3:-36927442 MS.gene71245:CDS
CAAATTTATCAGATATGCTT+AGG 0.466891 8.3:-36927500 MS.gene71245:CDS
AATTCAATGGTGGATTCATA+TGG 0.475276 8.3:-36929956 MS.gene71245:CDS
GAAGTGAGAAAAGATGGGTT+TGG 0.476292 8.3:-36929860 MS.gene71245:CDS
AAGGTCTAAATCCAAAGAAA+AGG 0.482803 8.3:-36929397 MS.gene71245:CDS
TGAGGGATACGTACATGTTA+GGG 0.486214 8.3:-36928735 MS.gene71245:CDS
CCACAGTTGGAACCTTTGGC+TGG 0.487866 8.3:-36927465 MS.gene71245:CDS
AGTTGGAACCTTTGGCTGGA+GGG 0.488222 8.3:-36927461 MS.gene71245:CDS
AAATGTACCTTGTTGCATCC+AGG 0.496384 8.3:+36928531 None:intergenic
AGCTTCTGATCCGCCTAATG+AGG 0.500508 8.3:-36928753 MS.gene71245:CDS
CTCTACCTCTACATGTGTCT+CGG 0.501570 8.3:-36929607 MS.gene71245:CDS
TAGAACAGTTGGAATGGGTT+GGG 0.502266 8.3:-36929813 MS.gene71245:CDS
ACATGTACGTATCCCTCATT+AGG 0.508463 8.3:+36928740 None:intergenic
AGGAGGGTCCTTTAGATTGG+AGG 0.508576 8.3:-36930063 MS.gene71245:CDS
TTACAAGACCCTCCTAGAGC+TGG 0.512634 8.3:+36929497 None:intergenic
TCCATGGAAAATTCTATGAT+GGG 0.513525 8.3:-36929995 MS.gene71245:CDS
GAAAAGGAAAAGAAATGGAC+AGG 0.513621 8.3:-36929381 MS.gene71245:intron
TTGTCCTTTGTGGTTTCACT+TGG 0.513815 8.3:+36928857 None:intergenic
GGCTATTTGTTGCTTGACCA+CGG 0.515569 8.3:+36928708 None:intergenic
TTACTCTCTCTGCAAGGCTA+TGG 0.516396 8.3:+36928687 None:intergenic
CGATGATGGTTCCCAAATGT+TGG 0.518846 8.3:-36929447 MS.gene71245:CDS
CCTGTAATGTCTCAAGATGA+AGG 0.520207 8.3:-36929524 MS.gene71245:CDS
AGAAACCCCAGAGCAGATGA+TGG 0.522386 8.3:+36927603 None:intergenic
CTCACAAACCTCCAATCTAA+AGG 0.533291 8.3:+36930055 None:intergenic
GCAGACTGTTCAACCACAGT+TGG 0.533598 8.3:-36927478 MS.gene71245:CDS
CTGGATTTGTTGATGCTGCA+AGG 0.542019 8.3:-36929697 MS.gene71245:CDS
TGATGATAGAACAGTTGGAA+TGG 0.544564 8.3:-36929819 MS.gene71245:CDS
ATAGAACAGTTGGAATGGGT+TGG 0.546913 8.3:-36929814 MS.gene71245:CDS
TAATCCATTGCAAGCAGCCA+AGG 0.549245 8.3:-36927418 MS.gene71245:CDS
GTTGAGCATCTTGGTAGTGA+TGG 0.549328 8.3:-36929581 MS.gene71245:CDS
TCTTTAAACCCTCCAGCCAA+AGG 0.552371 8.3:+36927453 None:intergenic
AACCTTGCAAATTCATCTGT+AGG 0.552403 8.3:-36930025 MS.gene71245:CDS
GGTTGTGATGATAGAACAGT+TGG 0.552863 8.3:-36929824 MS.gene71245:CDS
CCCAGAGCAGATGATGGACC+CGG 0.555611 8.3:+36927609 None:intergenic
TTCATGTCCTAATTCAATGG+TGG 0.556761 8.3:-36929966 MS.gene71245:CDS
CAGTTGGAACCTTTGGCTGG+AGG 0.557432 8.3:-36927462 MS.gene71245:CDS
GATGATAGAACAGTTGGAAT+GGG 0.563426 8.3:-36929818 MS.gene71245:CDS
ACTAACTCTGAAAACTTGGG+TGG 0.566336 8.3:-36929911 MS.gene71245:CDS
GATGAATCGAGTGGCGATGA+TGG 0.566803 8.3:-36929461 MS.gene71245:CDS
GTAGTAGTTCTATGATGAAG+AGG 0.567662 8.3:-36929784 MS.gene71245:CDS
AGAGGTAGAGCCATAGATTG+AGG 0.574801 8.3:+36929620 None:intergenic
CTGTAATGTCTCAAGATGAA+GGG 0.576780 8.3:-36929523 MS.gene71245:CDS
CTTACTAACTCTGAAAACTT+GGG 0.579332 8.3:-36929914 MS.gene71245:CDS
AGAGTTGAATGAATCAGTCC+TGG 0.580047 8.3:+36927534 None:intergenic
GGGTCTTGTAATGAAGCTGA+TGG 0.581611 8.3:-36929485 MS.gene71245:CDS
CAAATGTTGGATTGTGCAAG+TGG 0.582909 8.3:-36929434 MS.gene71245:CDS
GCTTCTGATCCGCCTAATGA+GGG 0.596006 8.3:-36928752 MS.gene71245:CDS
GATGAAGGGAAACCAGCTCT+AGG 0.597825 8.3:-36929509 MS.gene71245:CDS
TAGATATGATGAACTCATGT+GGG 0.599148 8.3:-36929646 MS.gene71245:CDS
TAGTAGTTCTATGATGAAGA+GGG 0.601347 8.3:-36929783 MS.gene71245:CDS
CATCATATCTACGAAACTCC+CGG 0.605426 8.3:+36929658 None:intergenic
TTTGAGGTAGATAGAAAGGA+GGG 0.606702 8.3:-36930079 MS.gene71245:CDS
CTGTTTGTAGGTCACTGGCA+AGG 0.607483 8.3:-36928423 MS.gene71245:intron
TCCATATTAAAATCCACATG+TGG 0.609814 8.3:+36927759 None:intergenic
GATAATGGAGGAAAAGCCAT+TGG 0.610652 8.3:+36927570 None:intergenic
TCATTCAACTCTACCTAACA+TGG 0.611728 8.3:-36927523 MS.gene71245:CDS
TGGTGATAGTGATAAAGCCA+CGG 0.611950 8.3:-36928894 MS.gene71245:CDS
AACCTACAGATGAATTTGCA+AGG 0.617947 8.3:+36930023 None:intergenic
CAGATTCTTCAACATCAACC+GGG 0.618225 8.3:-36927627 MS.gene71245:CDS
TGGTAGCTCAAGAGTTCCCG+TGG 0.619141 8.3:+36928877 None:intergenic
GGTGATAGTGATAAAGCCAC+GGG 0.623300 8.3:-36928893 MS.gene71245:CDS
CCAGCCAAAGGTTCCAACTG+TGG 0.623573 8.3:+36927465 None:intergenic
AAGGGAAACCAGCTCTAGGA+GGG 0.626130 8.3:-36929505 MS.gene71245:CDS
GAAGGGAAACCAGCTCTAGG+AGG 0.632084 8.3:-36929506 MS.gene71245:CDS
AACATCTTACTCTCTCTGCA+AGG 0.632535 8.3:+36928681 None:intergenic
TCATCATAGAACTACTACCA+AGG 0.632663 8.3:+36929789 None:intergenic
GTAGATATGATGAACTCATG+TGG 0.633019 8.3:-36929647 MS.gene71245:CDS
AGTTCTATGATGAAGAGGGA+TGG 0.633166 8.3:-36929779 MS.gene71245:CDS
GTCTCTTCAACAACAAGTTG+AGG 0.634721 8.3:-36928366 MS.gene71245:intron
TCTGGGAAGAATGCCAAACA+GGG 0.639672 8.3:-36928782 MS.gene71245:CDS
TGCAACAGTAAATCCACATG+TGG 0.646636 8.3:-36927772 MS.gene71245:CDS
GCAAACAGCTTCAACAACCA+AGG 0.656445 8.3:-36928807 MS.gene71245:CDS
TGATATGAATGTGATAATGG+AGG 0.657822 8.3:+36927558 None:intergenic
CGGGTCCATCATCTGCTCTG+GGG 0.660359 8.3:-36927608 MS.gene71245:CDS
GCAATGAAGTGAGAAAAGAT+GGG 0.662807 8.3:-36929865 MS.gene71245:CDS
GTTTCTCAGAGAAATGCCAA+TGG 0.663274 8.3:-36927586 MS.gene71245:CDS
TCTTGTACCTGGATGCAACA+AGG 0.668293 8.3:-36928538 MS.gene71245:intron
ACAGACCGAGACACATGTAG+AGG 0.676355 8.3:+36929602 None:intergenic
TGTACGTATCCCTCATTAGG+CGG 0.683851 8.3:+36928743 None:intergenic
GAAAACTGAGACAAACTCTG+AGG 0.709780 8.3:+36929728 None:intergenic
TTATCACATTCATATCAACC+AGG 0.710399 8.3:-36927552 MS.gene71245:CDS
GCTACCAAGTGAAACCACAA+AGG 0.714013 8.3:-36928861 MS.gene71245:CDS
GCTGATGGAGATGAATCGAG+TGG 0.745292 8.3:-36929470 MS.gene71245:CDS
GTACATGTTAGGGCTCGCCG+TGG 0.768171 8.3:-36928725 MS.gene71245:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTATTTTCTGTT+AGG - chr8.3:36928592-36928611 MS.gene71245:intron 10.0%
!! TAAAATAAAATAAACTCACA+TGG + chr8.3:36928578-36928597 None:intergenic 15.0%
!!! ATACAATAAATTGTTTTGTT+AGG - chr8.3:36929609-36929628 MS.gene71245:CDS 15.0%
!!! ATTTCTAGAAACATATATAA+GGG + chr8.3:36929651-36929670 None:intergenic 15.0%
!!! GACAATATTCAATTTTATAA+AGG + chr8.3:36929864-36929883 None:intergenic 15.0%
!!! TACAATAAATTGTTTTGTTA+GGG - chr8.3:36929610-36929629 MS.gene71245:CDS 15.0%
!! AAAAAATTGGTATTGTTTAG+CGG + chr8.3:36929364-36929383 None:intergenic 20.0%
!! AAAATTCATCATAGTGAATT+GGG - chr8.3:36928414-36928433 MS.gene71245:CDS 20.0%
!! AAATATGAATTATAAGCTGT+AGG - chr8.3:36928290-36928309 MS.gene71245:intron 20.0%
!! AAATTATTGCAATTTCAAAC+AGG + chr8.3:36929518-36929537 None:intergenic 20.0%
!! ATAGCAGAATTTGAAAAAAT+TGG + chr8.3:36929377-36929396 None:intergenic 20.0%
!! ATTATTTAGATTTCAACTGT+AGG - chr8.3:36928906-36928925 MS.gene71245:intron 20.0%
!! TAAATCCTCATATGAATATA+AGG + chr8.3:36929033-36929052 None:intergenic 20.0%
!!! ACAATAAATTGTTTTGTTAG+GGG - chr8.3:36929611-36929630 MS.gene71245:CDS 20.0%
!!! GATTTCTAGAAACATATATA+AGG + chr8.3:36929652-36929671 None:intergenic 20.0%
!!! TATTTTATTTTCTGTTAGGA+TGG - chr8.3:36928596-36928615 MS.gene71245:intron 20.0%
!!! TTTTAGTATAGTTGATACTA+TGG - chr8.3:36928172-36928191 MS.gene71245:intron 20.0%
! AAAAATATGGGAAAATGAGT+TGG + chr8.3:36929212-36929231 None:intergenic 25.0%
! ATATCCAAAATAGATACACT+AGG - chr8.3:36928453-36928472 MS.gene71245:intron 25.0%
! ATCTTTGTTACAATTGGTAA+AGG - chr8.3:36929573-36929592 MS.gene71245:CDS 25.0%
! CAAAATTCATCATAGTGAAT+TGG - chr8.3:36928413-36928432 MS.gene71245:CDS 25.0%
! CAAATTTATCAGATATGCTT+AGG - chr8.3:36930010-36930029 MS.gene71245:CDS 25.0%
! CGAGAAGAAAAAAAATTGTT+TGG - chr8.3:36928870-36928889 MS.gene71245:CDS 25.0%
! TATCCAAAATAGATACACTA+GGG - chr8.3:36928454-36928473 MS.gene71245:intron 25.0%
! TTGGATGATTTAATAATCAG+TGG + chr8.3:36929193-36929212 None:intergenic 25.0%
!! AACTCATTTTCCCATATTTT+TGG - chr8.3:36929211-36929230 MS.gene71245:intron 25.0%
!! ACTAGAGCTATACTAATTTT+AGG - chr8.3:36928369-36928388 MS.gene71245:CDS 25.0%
!! ACTATCTATTTTCTGAATTG+TGG - chr8.3:36928226-36928245 MS.gene71245:intron 25.0%
!! ATCATTGTTATTCTGTTTGT+AGG - chr8.3:36929075-36929094 MS.gene71245:intron 25.0%
!! TAACTGTTATTTGACAACAT+AGG - chr8.3:36929419-36929438 MS.gene71245:CDS 25.0%
!!! AAATTTTGAGGTAGATAGAA+AGG - chr8.3:36927427-36927446 MS.gene71245:CDS 25.0%
!!! ATGTGGATTTTAATATGGAA+CGG - chr8.3:36929755-36929774 MS.gene71245:CDS 25.0%
!!! ATTGTAAGCTTTAAAATTGC+TGG + chr8.3:36929820-36929839 None:intergenic 25.0%
AAAATAAACTCACATGGACA+AGG + chr8.3:36928572-36928591 None:intergenic 30.0%
AAATAAACTCACATGGACAA+GGG + chr8.3:36928571-36928590 None:intergenic 30.0%
AAGGTCTAAATCCAAAGAAA+AGG - chr8.3:36928113-36928132 MS.gene71245:intron 30.0%
AATTGTAACAAAGATGACAC+CGG + chr8.3:36929568-36929587 None:intergenic 30.0%
ATACTATGGTTATCAATCTG+AGG - chr8.3:36928186-36928205 MS.gene71245:intron 30.0%
ATGTTAATGAGAAACTCTAC+TGG + chr8.3:36929470-36929489 None:intergenic 30.0%
ATTGATAAAAACTGCCAAGA+GGG + chr8.3:36929712-36929731 None:intergenic 30.0%
CACTACCTTATATTCATATG+AGG - chr8.3:36929025-36929044 MS.gene71245:intron 30.0%
CCAAAGAAAAGGAAAAGAAA+TGG - chr8.3:36928124-36928143 MS.gene71245:intron 30.0%
GAAGCAGAATCCAAAAATAT+GGG + chr8.3:36929224-36929243 None:intergenic 30.0%
GGATTTAGACCTTTAATAGA+AGG + chr8.3:36928106-36928125 None:intergenic 30.0%
GTCATTAATCAAACGACAAA+AGG - chr8.3:36928340-36928359 MS.gene71245:intron 30.0%
GTGAATTGGGATTAAAAATG+AGG - chr8.3:36928427-36928446 MS.gene71245:intron 30.0%
TAGATATGATGAACTCATGT+GGG - chr8.3:36927864-36927883 MS.gene71245:intron 30.0%
TATGAATCCACCATTGAATT+AGG + chr8.3:36927554-36927573 None:intergenic 30.0%
TCCATATTAAAATCCACATG+TGG + chr8.3:36929754-36929773 None:intergenic 30.0%
TCCATGGAAAATTCTATGAT+GGG - chr8.3:36927515-36927534 MS.gene71245:CDS 30.0%
TCTGATAAATTTGCCATGTT+AGG + chr8.3:36930003-36930022 None:intergenic 30.0%
TGAAGCAGAATCCAAAAATA+TGG + chr8.3:36929225-36929244 None:intergenic 30.0%
TTATCACATTCATATCAACC+AGG - chr8.3:36929958-36929977 MS.gene71245:CDS 30.0%
TTCATATGGTCCTAATTTCT+TGG - chr8.3:36927568-36927587 MS.gene71245:CDS 30.0%
TTCCATGGAAAATTCTATGA+TGG - chr8.3:36927514-36927533 MS.gene71245:CDS 30.0%
TTCTTCATGTCCTAATTCAA+TGG - chr8.3:36927541-36927560 MS.gene71245:CDS 30.0%
! AATCCCTAGTGTATCTATTT+TGG + chr8.3:36928460-36928479 None:intergenic 30.0%
! AGTTTTCAGAGTTAGTAAGA+AGG + chr8.3:36927595-36927614 None:intergenic 30.0%
! CCATTTCTTTTCCTTTTCTT+TGG + chr8.3:36928127-36928146 None:intergenic 30.0%
! CTTACTAACTCTGAAAACTT+GGG - chr8.3:36927596-36927615 MS.gene71245:CDS 30.0%
! GAGTGAAAGTGAAAATTTTG+AGG - chr8.3:36927415-36927434 MS.gene71245:CDS 30.0%
! GGTTGATATGAATGTGATAA+TGG + chr8.3:36929958-36929977 None:intergenic 30.0%
! GTACATTTAAAACTTGAGAC+TGG - chr8.3:36928994-36929013 MS.gene71245:intron 30.0%
! TAGTAGTTCTATGATGAAGA+GGG - chr8.3:36927727-36927746 MS.gene71245:intron 30.0%
! TCTTACTAACTCTGAAAACT+TGG - chr8.3:36927595-36927614 MS.gene71245:CDS 30.0%
! TTGTCGTTTGATTAATGACT+TGG + chr8.3:36928339-36928358 None:intergenic 30.0%
!! AATTCAATGGTGGATTCATA+TGG - chr8.3:36927554-36927573 MS.gene71245:CDS 30.0%
!! TCCACATGTGGATTTTAATA+TGG - chr8.3:36929750-36929769 MS.gene71245:CDS 30.0%
!! TGATATGAATGTGATAATGG+AGG + chr8.3:36929955-36929974 None:intergenic 30.0%
AACCTACAGATGAATTTGCA+AGG + chr8.3:36927490-36927509 None:intergenic 35.0%
AACCTTGCAAATTCATCTGT+AGG - chr8.3:36927485-36927504 MS.gene71245:CDS 35.0%
AACTGTGAGAGCAGAAAAAA+GGG - chr8.3:36928674-36928693 MS.gene71245:intron 35.0%
AATAAACTCACATGGACAAG+GGG + chr8.3:36928570-36928589 None:intergenic 35.0%
ACTGCTGATGATTTAAATGC+CGG - chr8.3:36929546-36929565 MS.gene71245:CDS 35.0%
ACTTGCACAATCCAACATTT+GGG + chr8.3:36928077-36928096 None:intergenic 35.0%
AGCAATGAAGTGAGAAAAGA+TGG - chr8.3:36927644-36927663 MS.gene71245:intron 35.0%
AGTGGAGAACCTTCTATTAA+AGG - chr8.3:36928094-36928113 MS.gene71245:intron 35.0%
ATATAAGGGACAACTGACAA+TGG + chr8.3:36929637-36929656 None:intergenic 35.0%
CATTGATAAAAACTGCCAAG+AGG + chr8.3:36929713-36929732 None:intergenic 35.0%
CTGTAATGTCTCAAGATGAA+GGG - chr8.3:36927987-36928006 MS.gene71245:intron 35.0%
GAAAAGGAAAAGAAATGGAC+AGG - chr8.3:36928129-36928148 MS.gene71245:intron 35.0%
GATGATAGAACAGTTGGAAT+GGG - chr8.3:36927692-36927711 MS.gene71245:intron 35.0%
GCAATGAAGTGAGAAAAGAT+GGG - chr8.3:36927645-36927664 MS.gene71245:intron 35.0%
GTAAGAAGGTCCAAGAAATT+AGG + chr8.3:36927581-36927600 None:intergenic 35.0%
GTAGATATGATGAACTCATG+TGG - chr8.3:36927863-36927882 MS.gene71245:intron 35.0%
TCAGATTCTTCAACATCAAC+CGG - chr8.3:36929882-36929901 MS.gene71245:CDS 35.0%
TCATCATAGAACTACTACCA+AGG + chr8.3:36927724-36927743 None:intergenic 35.0%
TCATTCAACTCTACCTAACA+TGG - chr8.3:36929987-36930006 MS.gene71245:CDS 35.0%
TGATGATAGAACAGTTGGAA+TGG - chr8.3:36927691-36927710 MS.gene71245:intron 35.0%
TGCAAATTCATCTGTAGGTT+TGG - chr8.3:36927490-36927509 MS.gene71245:CDS 35.0%
TTAGATTTCAACTGTAGGTG+AGG - chr8.3:36928911-36928930 MS.gene71245:intron 35.0%
TTATTCTGTTTGTAGGTCAC+TGG - chr8.3:36929082-36929101 MS.gene71245:intron 35.0%
TTCATGTCCTAATTCAATGG+TGG - chr8.3:36927544-36927563 MS.gene71245:CDS 35.0%
TTTCTTCAAGATCTTGTACC+TGG - chr8.3:36928961-36928980 MS.gene71245:intron 35.0%
! ACCCATCATAGAATTTTCCA+TGG + chr8.3:36927519-36927538 None:intergenic 35.0%
! GGTGGTTTTTGTGATGTTAA+TGG - chr8.3:36927617-36927636 MS.gene71245:CDS 35.0%
! GGTGTCATCTTTGTTACAAT+TGG - chr8.3:36929567-36929586 MS.gene71245:CDS 35.0%
! GTAGTAGTTCTATGATGAAG+AGG - chr8.3:36927726-36927745 MS.gene71245:intron 35.0%
! TTCAACAACAAGTTGAGGTA+TGG - chr8.3:36929149-36929168 MS.gene71245:intron 35.0%
! TTTGAGGTAGATAGAAAGGA+GGG - chr8.3:36927431-36927450 MS.gene71245:CDS 35.0%
!! TCTCAGTTTTCAACTGATTC+TGG - chr8.3:36927794-36927813 MS.gene71245:intron 35.0%
!! TGCAATGGATTAACCTGATT+TGG + chr8.3:36930084-36930103 None:intergenic 35.0%
!!! TTTTGAGGTAGATAGAAAGG+AGG - chr8.3:36927430-36927449 MS.gene71245:CDS 35.0%
AAATGTACCTTGTTGCATCC+AGG + chr8.3:36928982-36929001 None:intergenic 40.0%
AACATCTTACTCTCTCTGCA+AGG + chr8.3:36928832-36928851 None:intergenic 40.0%
ACATGTACGTATCCCTCATT+AGG + chr8.3:36928773-36928792 None:intergenic 40.0%
AGAGTTGAATGAATCAGTCC+TGG + chr8.3:36929979-36929998 None:intergenic 40.0%
AGGGTTTAAAGAGCCAAATC+AGG - chr8.3:36930068-36930087 MS.gene71245:CDS 40.0%
AGTCACTTCTTCGATCAATC+TGG + chr8.3:36927964-36927983 None:intergenic 40.0%
AGTTCTATGATGAAGAGGGA+TGG - chr8.3:36927731-36927750 MS.gene71245:intron 40.0%
ATAAACTCACATGGACAAGG+GGG + chr8.3:36928569-36928588 None:intergenic 40.0%
ATAGAACAGTTGGAATGGGT+TGG - chr8.3:36927696-36927715 MS.gene71245:intron 40.0%
ATGAGGGATACGTACATGTT+AGG - chr8.3:36928774-36928793 MS.gene71245:CDS 40.0%
ATGTGGGATTCCTCAATCTA+TGG - chr8.3:36927880-36927899 MS.gene71245:intron 40.0%
CAAATGTTGGATTGTGCAAG+TGG - chr8.3:36928076-36928095 MS.gene71245:intron 40.0%
CAACTGTGAGAGCAGAAAAA+AGG - chr8.3:36928673-36928692 MS.gene71245:intron 40.0%
CACTTGCACAATCCAACATT+TGG + chr8.3:36928078-36928097 None:intergenic 40.0%
CAGATTCTTCAACATCAACC+GGG - chr8.3:36929883-36929902 MS.gene71245:CDS 40.0%
CATCATATCTACGAAACTCC+CGG + chr8.3:36927855-36927874 None:intergenic 40.0%
CCTGTAATGTCTCAAGATGA+AGG - chr8.3:36927986-36928005 MS.gene71245:intron 40.0%
CCTTCATCTTGAGACATTAC+AGG + chr8.3:36927989-36928008 None:intergenic 40.0%
CTCACAAACCTCCAATCTAA+AGG + chr8.3:36927458-36927477 None:intergenic 40.0%
CTGAGGTTCAAATCGTGAAT+CGG - chr8.3:36928203-36928222 MS.gene71245:intron 40.0%
CTTCTTCGATCAATCTGGAT+TGG + chr8.3:36927959-36927978 None:intergenic 40.0%
GAAAACTGAGACAAACTCTG+AGG + chr8.3:36927785-36927804 None:intergenic 40.0%
GACTAGAACCGTACATCTTT+AGG + chr8.3:36929787-36929806 None:intergenic 40.0%
GGTTGTGATGATAGAACAGT+TGG - chr8.3:36927686-36927705 MS.gene71245:intron 40.0%
GTCTCTTCAACAACAAGTTG+AGG - chr8.3:36929144-36929163 MS.gene71245:intron 40.0%
GTGAACCTCAATATAGTCGT+AGG - chr8.3:36928312-36928331 MS.gene71245:intron 40.0%
GTTTCTCAGAGAAATGCCAA+TGG - chr8.3:36929924-36929943 MS.gene71245:CDS 40.0%
TATAGCACCAATAGCGCAAA+TGG + chr8.3:36929401-36929420 None:intergenic 40.0%
TGAGGGATACGTACATGTTA+GGG - chr8.3:36928775-36928794 MS.gene71245:CDS 40.0%
TGCAACAGTAAATCCACATG+TGG - chr8.3:36929738-36929757 MS.gene71245:CDS 40.0%
TTGGACCTACGACTATATTG+AGG + chr8.3:36928320-36928339 None:intergenic 40.0%
TTGTCCTTTGTGGTTTCACT+TGG + chr8.3:36928656-36928675 None:intergenic 40.0%
TTGTTTAGCGGTACACTTAG+AGG + chr8.3:36929352-36929371 None:intergenic 40.0%
! ACTAACTCTGAAAACTTGGG+TGG - chr8.3:36927599-36927618 MS.gene71245:CDS 40.0%
! AGGGATGGTGTTTTACCAAA+TGG - chr8.3:36927746-36927765 MS.gene71245:CDS 40.0%
! ATCTGTAGGTTTGGTTTCCA+TGG - chr8.3:36927499-36927518 MS.gene71245:CDS 40.0%
! GAAGTGAGAAAAGATGGGTT+TGG - chr8.3:36927650-36927669 MS.gene71245:intron 40.0%
! GGATGGTGTTTTACCAAATG+GGG - chr8.3:36927748-36927767 MS.gene71245:CDS 40.0%
! GGGATGGTGTTTTACCAAAT+GGG - chr8.3:36927747-36927766 MS.gene71245:CDS 40.0%
! TAGAACAGTTGGAATGGGTT+GGG - chr8.3:36927697-36927716 MS.gene71245:intron 40.0%
! TGACACATTTTTCTCCCTCT+TGG - chr8.3:36929695-36929714 MS.gene71245:CDS 40.0%
! TGGTGATAGTGATAAAGCCA+CGG - chr8.3:36928616-36928635 MS.gene71245:intron 40.0%
!! GATAATGGAGGAAAAGCCAT+TGG + chr8.3:36929943-36929962 None:intergenic 40.0%
!!! AGGATGTCATGTTTTAGTGC+CGG - chr8.3:36927833-36927852 MS.gene71245:intron 40.0%
AGAAGCTTGAGAACCCTGTT+TGG + chr8.3:36928744-36928763 None:intergenic 45.0%
AGAGGTAGAGCCATAGATTG+AGG + chr8.3:36927893-36927912 None:intergenic 45.0%
CTCTACCTCTACATGTGTCT+CGG - chr8.3:36927903-36927922 MS.gene71245:intron 45.0%
CTTCAACAACCAAGGCTTCT+GGG - chr8.3:36928711-36928730 MS.gene71245:CDS 45.0%
GAAAGGAGGGTCCTTTAGAT+TGG - chr8.3:36927444-36927463 MS.gene71245:CDS 45.0%
GCAAACAGCTTCAACAACCA+AGG - chr8.3:36928703-36928722 MS.gene71245:CDS 45.0%
GCTACCAAGTGAAACCACAA+AGG - chr8.3:36928649-36928668 MS.gene71245:intron 45.0%
GGCTGCTTCCTAAAGATGTA+CGG - chr8.3:36929776-36929795 MS.gene71245:CDS 45.0%
GGGGGAAGCAGTATAAATCA+TGG + chr8.3:36928551-36928570 None:intergenic 45.0%
TAATCCATTGCAAGCAGCCA+AGG - chr8.3:36930092-36930111 MS.gene71245:CDS 45.0%
TCAACCACAGTTGGAACCTT+TGG - chr8.3:36930041-36930060 MS.gene71245:CDS 45.0%
TCTGGGAAGAATGCCAAACA+GGG - chr8.3:36928728-36928747 MS.gene71245:CDS 45.0%
TCTTGTACCTGGATGCAACA+AGG - chr8.3:36928972-36928991 MS.gene71245:intron 45.0%
TCTTTAAACCCTCCAGCCAA+AGG + chr8.3:36930060-36930079 None:intergenic 45.0%
TGTACGTATCCCTCATTAGG+CGG + chr8.3:36928770-36928789 None:intergenic 45.0%
TTACTCTCTCTGCAAGGCTA+TGG + chr8.3:36928826-36928845 None:intergenic 45.0%
! CGATGATGGTTCCCAAATGT+TGG - chr8.3:36928063-36928082 MS.gene71245:intron 45.0%
! GGCTATTTGTTGCTTGACCA+CGG + chr8.3:36928805-36928824 None:intergenic 45.0%
! GGGTCTTGTAATGAAGCTGA+TGG - chr8.3:36928025-36928044 MS.gene71245:intron 45.0%
! GGTGATAGTGATAAAGCCAC+GGG - chr8.3:36928617-36928636 MS.gene71245:intron 45.0%
! TGCTATGCCATTTGCGCTAT+TGG - chr8.3:36929391-36929410 MS.gene71245:CDS 45.0%
! TTCTGGGAAGAATGCCAAAC+AGG - chr8.3:36928727-36928746 MS.gene71245:CDS 45.0%
!! CTGGATTTGTTGATGCTGCA+AGG - chr8.3:36927813-36927832 MS.gene71245:intron 45.0%
!! GTTGAGCATCTTGGTAGTGA+TGG - chr8.3:36927929-36927948 MS.gene71245:intron 45.0%
!!! GGATGTCATGTTTTAGTGCC+GGG - chr8.3:36927834-36927853 MS.gene71245:intron 45.0%
!!! GGGTTTGGTTTTGCAAGAGT+TGG - chr8.3:36927665-36927684 MS.gene71245:intron 45.0%
ACAAGTGTGCTATAGCGCTG+TGG + chr8.3:36929304-36929323 None:intergenic 50.0%
ACAGACCGAGACACATGTAG+AGG + chr8.3:36927911-36927930 None:intergenic 50.0%
AGAAACCCCAGAGCAGATGA+TGG + chr8.3:36929910-36929929 None:intergenic 50.0%
AGCTTCTGATCCGCCTAATG+AGG - chr8.3:36928757-36928776 MS.gene71245:CDS 50.0%
AGGAGGGTCCTTTAGATTGG+AGG - chr8.3:36927447-36927466 MS.gene71245:CDS 50.0%
AGTTGGAACCTTTGGCTGGA+GGG - chr8.3:36930049-36930068 MS.gene71245:CDS 50.0%
CTGTTTGTAGGTCACTGGCA+AGG - chr8.3:36929087-36929106 MS.gene71245:intron 50.0%
GATGAAGGGAAACCAGCTCT+AGG - chr8.3:36928001-36928020 MS.gene71245:intron 50.0%
GATGAATCGAGTGGCGATGA+TGG - chr8.3:36928049-36928068 MS.gene71245:intron 50.0%
GCAGACTGTTCAACCACAGT+TGG - chr8.3:36930032-36930051 MS.gene71245:CDS 50.0%
GCTATAGCGCTGTGGAATAG+CGG + chr8.3:36929296-36929315 None:intergenic 50.0%
GCTCTCACAGTTGTCCTTTG+TGG + chr8.3:36928666-36928685 None:intergenic 50.0%
GCTGATGGAGATGAATCGAG+TGG - chr8.3:36928040-36928059 MS.gene71245:intron 50.0%
GCTTCAACAACCAAGGCTTC+TGG - chr8.3:36928710-36928729 MS.gene71245:CDS 50.0%
GCTTCTGATCCGCCTAATGA+GGG - chr8.3:36928758-36928777 MS.gene71245:CDS 50.0%
GGAAACATCTCAGCCCCATT+TGG + chr8.3:36927764-36927783 None:intergenic 50.0%
TCTCGGTCTGTTGAGCATCT+TGG - chr8.3:36927920-36927939 MS.gene71245:intron 50.0%
TTACAAGACCCTCCTAGAGC+TGG + chr8.3:36928016-36928035 None:intergenic 50.0%
!! AAGGGAAACCAGCTCTAGGA+GGG - chr8.3:36928005-36928024 MS.gene71245:intron 50.0%
CAGTCCTTGGCTGCTTGCAA+TGG + chr8.3:36930099-36930118 None:intergenic 55.0%
CAGTTGGAACCTTTGGCTGG+AGG - chr8.3:36930048-36930067 MS.gene71245:CDS 55.0%
CCACAGTTGGAACCTTTGGC+TGG - chr8.3:36930045-36930064 MS.gene71245:CDS 55.0%
CCAGCCAAAGGTTCCAACTG+TGG + chr8.3:36930048-36930067 None:intergenic 55.0%
GGCATTCTTCCCAGAAGCCT+TGG + chr8.3:36928723-36928742 None:intergenic 55.0%
TGGTAGCTCAAGAGTTCCCG+TGG + chr8.3:36928636-36928655 None:intergenic 55.0%
!! GAAGGGAAACCAGCTCTAGG+AGG - chr8.3:36928004-36928023 MS.gene71245:intron 55.0%
!! GTTGGAATGGGTTGGGACCT+TGG - chr8.3:36927704-36927723 MS.gene71245:intron 55.0%
ACCGGGTCCATCATCTGCTC+TGG - chr8.3:36929900-36929919 MS.gene71245:CDS 60.0%
CCGGGTCCATCATCTGCTCT+GGG - chr8.3:36929901-36929920 MS.gene71245:CDS 60.0%
CGGGTCCATCATCTGCTCTG+GGG - chr8.3:36929902-36929921 MS.gene71245:CDS 60.0%
GTACATGTTAGGGCTCGCCG+TGG - chr8.3:36928785-36928804 MS.gene71245:CDS 60.0%
!! CCCAGAGCAGATGATGGACC+CGG + chr8.3:36929904-36929923 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 36927413 36930119 36927413 ID=MS.gene71245
chr8.3 mRNA 36927413 36930119 36927413 ID=MS.gene71245.t1;Parent=MS.gene71245
chr8.3 exon 36929382 36930119 36929382 ID=MS.gene71245.t1.exon1;Parent=MS.gene71245.t1
chr8.3 CDS 36929382 36930119 36929382 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
chr8.3 exon 36928691 36928918 36928691 ID=MS.gene71245.t1.exon2;Parent=MS.gene71245.t1
chr8.3 CDS 36928691 36928918 36928691 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
chr8.3 exon 36928539 36928604 36928539 ID=MS.gene71245.t1.exon3;Parent=MS.gene71245.t1
chr8.3 CDS 36928539 36928604 36928539 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
chr8.3 exon 36928367 36928435 36928367 ID=MS.gene71245.t1.exon4;Parent=MS.gene71245.t1
chr8.3 CDS 36928367 36928435 36928367 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
chr8.3 exon 36927740 36927811 36927740 ID=MS.gene71245.t1.exon5;Parent=MS.gene71245.t1
chr8.3 CDS 36927740 36927811 36927740 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
chr8.3 exon 36927413 36927646 36927413 ID=MS.gene71245.t1.exon6;Parent=MS.gene71245.t1
chr8.3 CDS 36927413 36927646 36927413 ID=cds.MS.gene71245.t1;Parent=MS.gene71245.t1
Gene Sequence

>MS.gene71245

ATGAGTGAAAGTGAAAATTTTGAGGTAGATAGAAAGGAGGGTCCTTTAGATTGGAGGTTTGTGAGTTGCAACAACCTTGCAAATTCATCTGTAGGTTTGGTTTCCATGGAAAATTCTATGATGGGTTCTTCTTCATGTCCTAATTCAATGGTGGATTCATATGGTCCTAATTTCTTGGACCTTCTTACTAACTCTGAAAACTTGGGTGGTTTTTGTGATGTTAATGGTCATAGCAATGAAGTGAGAAAAGATGGGTTTGGTTTTGCAAGAGTTGGTTGTGATGATAGAACAGTTGGAATGGGTTGGGACCTTGGTAGTAGTTCTATGATGAAGAGGGATGGTGTTTTACCAAATGGGGCTGAGATGTTTCCTCAGAGTTTGTCTCAGTTTTCAACTGATTCTGGATTTGTTGATGCTGCAAGGATGTCATGTTTTAGTGCCGGGAGTTTCGTAGATATGATGAACTCATGTGGGATTCCTCAATCTATGGCTCTACCTCTACATGTGTCTCGGTCTGTTGAGCATCTTGGTAGTGATGGAAGTCCAATCCAGATTGATCGAAGAAGTGACTGTCCTGTAATGTCTCAAGATGAAGGGAAACCAGCTCTAGGAGGGTCTTGTAATGAAGCTGATGGAGATGAATCGAGTGGCGATGATGGTTCCCAAATGTTGGATTGTGCAAGTGGAGAACCTTCTATTAAAGGTCTAAATCCAAAGAAAAGGAAAAGAAATGGACAGGTATATTTCTTTGAGTTGTATTTTTAGTATAGTTGATACTATGGTTATCAATCTGAGGTTCAAATCGTGAATCGGACTATCTATTTTCTGAATTGTGGATTGAATCTTTTATTATGAATCGTAAGATGAATTACGAATAAAATATGAATTATAAGCTGTAGGTGAACCTCAATATAGTCGTAGGTCCAAGTCATTAATCAAACGACAAAAGGAAAGTGACTAGAGCTATACTAATTTTAGGTTGTGATTCATTAGAAACAATCAAAATTCATCATAGTGAATTGGGATTAAAAATGAGGTTCATATCCAAAATAGATACACTAGGGATTTGTATTGATCGAGTTTAGTTTTGTATCGAATCAAGATACAACTCACATGTATCGTAATATATTGATAACCATGATTTATACTGCTTCCCCCTTGTCCATGTGAGTTTATTTTATTTTATTTTATTTTCTGTTAGGATGGTGATAGTGATAAAGCCACGGGAACTCTTGAGCTACCAAGTGAAACCACAAAGGACAACTGTGAGAGCAGAAAAAAGGGAAAGCAGCAAACAGCTTCAACAACCAAGGCTTCTGGGAAGAATGCCAAACAGGGTTCTCAAGCTTCTGATCCGCCTAATGAGGGATACGTACATGTTAGGGCTCGCCGTGGTCAAGCAACAAATAGCCATAGCCTTGCAGAGAGAGTAAGATGTTCTAACTAAATTTATGTGACGAGAAGAAAAAAAATTGTTTGGATTTAGATTTCTAATTATTTAGATTTCAACTGTAGGTGAGGAGAGAAAAGATCAGTGAAAGAATGAAGTTTCTTCAAGATCTTGTACCTGGATGCAACAAGGTACATTTAAAACTTGAGACTGGAAGATTTTCACTACCTTATATTCATATGAGGATTTAGAGTGTTGTTTTCTTTTGATATATCATTGTTATTCTGTTTGTAGGTCACTGGCAAGGCAGTGATGCTAGATGAAATCATTAATTATGTACAGTCTCTTCAACAACAAGTTGAGGTATGGCTAAAAGCATATTTCTAACCACTGATTATTAAATCATCCAACTCATTTTCCCATATTTTTGGATTCTGCTTCAGTAAGTGTTGTCAATCATGAATCAGAGAAAATAACATTTGTTTAAATTCCGCTATTCCACAGCGCTATAGCACACTTGTATAGCTTCTATTTGATAACACTTGTCCTCTAAGTGTACCGCTAAACAATACCAATTTTTTCAAATTCTGCTATGCCATTTGCGCTATTGGTGCTATAACTGTTATTTGACAACATAGGCTTCTGTACGCTATCAGCATATGTTCCAGTAGAGTTTCTCATTAACATCGTTGACTGTTATAATGTTATTCTACCTGTTTGAAATTGCAATAATTTGAACGATCACTGCTGATGATTTAAATGCCGGTGTCATCTTTGTTACAATTGGTAAAGGATTACGAATAATTATACAATAAATTGTTTTGTTAGGGGCCATTGTCAGTTGTCCCTTATATATGTTTCTAGAAATCTATGCATCTTTTTCAGTTGAAGTTGACACATTTTTCTCCCTCTTGGCAGTTTTTATCAATGAAACTTGCAACAGTAAATCCACATGTGGATTTTAATATGGAACGGCTGCTTCCTAAAGATGTACGGTTCTAGTCAATCTGCATACCAGCAATTTTAAAGCTTACAATTAGCTATATATGCTTGACTGACCTTTATAAAATTGAATATTGTCAGATTCTTCAACATCAACCGGGTCCATCATCTGCTCTGGGGTTTCTCAGAGAAATGCCAATGGCTTTTCCTCCATTATCACATTCATATCAACCAGGACTGATTCATTCAACTCTACCTAACATGGCAAATTTATCAGATATGCTTAGGCAGACTGTTCAACCACAGTTGGAACCTTTGGCTGGAGGGTTTAAAGAGCCAAATCAGGTTAATCCATTGCAAGCAGCCAAGGACTGA
Protein sequence

>MS.gene71245.t1

MSESENFEVDRKEGPLDWRFVSCNNLANSSVGLVSMENSMMGSSSCPNSMVDSYGPNFLDLLTNSENLGGFCDVNGHSNEVRKDGFGFARVGCDDRTVGMGWDLGSSSMMKRDGVLPNGAEMFPQSLSQFSTDSGFVDAARMSCFSAGSFVDMMNSCGIPQSMALPLHVSRSVEHLGSDGSPIQIDRRSDCPVMSQDEGKPALGGSCNEADGDESSGDDGSQMLDCASGEPSIKGLNPKKRKRNGQDGDSDKATGTLELPSETTKDNCESRKKGKQQTASTTKASGKNAKQGSQASDPPNEGYVHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPHVDFNMERLLPKDILQHQPGPSSALGFLREMPMAFPPLSHSYQPGLIHSTLPNMANLSDMLRQTVQPQLEPLAGGFKEPNQVNPLQAAKD