AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71270.t1 MTR_1g070355 44.178 292 118 3 77 327 22 309 6.49e-68 234
MS.gene71270.t1 MTR_6g022070 33.648 318 173 6 387 698 1 286 2.07e-48 174
MS.gene71270.t1 MTR_4g050510 44.865 185 62 3 84 268 55 199 9.95e-40 145
MS.gene71270.t1 MTR_5g076070 35.586 222 102 1 139 319 59 280 1.29e-37 142
MS.gene71270.t1 MTR_6g022080 34.862 218 136 2 64 280 20 232 1.94e-33 129
MS.gene71270.t1 MTR_0060s0090 55.294 85 38 0 136 220 59 143 2.67e-29 114
MS.gene71270.t1 MTR_6g463250 40.741 135 64 2 86 220 42 160 3.56e-26 106
MS.gene71270.t1 MTR_2g101460 44.882 127 70 0 113 239 35 161 1.05e-25 105
MS.gene71270.t1 MTR_2g101460 44.882 127 70 0 113 239 25 151 2.74e-25 104
MS.gene71270.t1 MTR_7g446520 26.708 322 166 6 54 327 8 307 2.64e-24 105
MS.gene71270.t1 MTR_3g448650 44.898 98 54 0 113 210 62 159 9.03e-24 99.4
MS.gene71270.t1 MTR_6g051425 28.358 268 141 5 141 360 25 289 1.77e-23 102
MS.gene71270.t1 MTR_4g035820 26.639 244 130 5 139 335 81 322 9.21e-21 97.8
MS.gene71270.t1 MTR_6g053140 33.721 172 93 3 56 226 38 189 5.66e-20 89.4
MS.gene71270.t1 MTR_8g066350 32.927 164 88 2 77 240 27 168 3.19e-19 85.9
MS.gene71270.t1 MTR_7g028855 27.530 247 164 5 28 274 30 261 4.50e-19 91.3
MS.gene71270.t1 MTR_3g450850 27.778 234 114 7 139 319 53 284 5.69e-19 89.7
MS.gene71270.t1 MTR_1g052260 37.288 118 68 1 97 208 34 151 5.17e-18 82.0
MS.gene71270.t1 MTR_7g446480 28.571 175 82 1 211 342 141 315 1.58e-15 78.6
MS.gene71270.t1 MTR_7g446510 28.571 175 82 1 211 342 156 330 7.11e-15 77.0
MS.gene71270.t1 MTR_0604s0020 44.595 74 41 0 144 217 3 76 8.73e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71270.t1 AT4G38180 29.103 457 274 5 129 536 69 524 1.60e-65 232
MS.gene71270.t1 AT3G06250 24.710 603 332 17 132 646 189 757 4.73e-53 196
MS.gene71270.t1 AT3G06250 24.710 603 332 17 132 646 189 757 4.73e-53 196
MS.gene71270.t1 AT3G06250 24.710 603 332 17 132 646 189 757 4.73e-53 196
MS.gene71270.t1 AT5G18960 24.918 610 325 18 132 646 210 781 6.75e-53 196
MS.gene71270.t1 AT5G18960 24.918 610 325 18 132 646 210 781 6.75e-53 196
MS.gene71270.t1 AT3G22170 27.483 433 276 11 129 524 66 497 1.05e-49 187
MS.gene71270.t1 AT3G22170 27.483 433 276 11 129 524 66 497 1.05e-49 187
MS.gene71270.t1 AT1G10240 25.956 497 283 10 139 572 54 528 1.75e-48 182
MS.gene71270.t1 AT1G10240 25.956 497 283 10 139 572 54 528 1.75e-48 182
MS.gene71270.t1 AT4G15090 26.350 463 279 7 128 536 46 500 1.63e-47 181
MS.gene71270.t1 AT4G15090 26.350 463 279 7 128 536 46 500 1.63e-47 181
MS.gene71270.t1 AT4G15090 26.350 463 279 7 128 536 46 500 1.63e-47 181
MS.gene71270.t1 AT4G15090 26.350 463 279 7 128 536 46 500 1.63e-47 181
MS.gene71270.t1 AT4G15090 26.350 463 279 7 128 536 46 500 1.63e-47 181
MS.gene71270.t1 AT1G52520 27.602 442 269 11 137 536 89 521 1.56e-45 174
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 1 405 5.97e-45 172
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 6.12e-45 173
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 6.12e-45 173
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 6.25e-45 173
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 6.25e-45 173
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 6.25e-45 173
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 1 405 7.16e-45 172
MS.gene71270.t1 AT2G32250 26.316 418 279 6 137 538 42 446 7.50e-45 172
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 18 425 1.89e-42 165
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 18 425 1.89e-42 165
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 18 425 1.98e-42 165
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 32 439 2.12e-42 165
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 18 425 2.90e-42 165
MS.gene71270.t1 AT4G19990 27.073 410 273 10 128 513 18 425 2.90e-42 165
MS.gene71270.t1 AT1G80010 28.218 404 219 11 137 481 99 490 1.23e-41 162
MS.gene71270.t1 AT1G76320 24.045 445 285 8 137 536 1 437 2.55e-41 161
MS.gene71270.t1 AT1G76320 24.045 445 285 8 137 536 1 437 2.70e-41 161
MS.gene71270.t1 AT1G76320 24.045 445 285 8 137 536 1 437 2.79e-41 161
MS.gene71270.t1 AT1G76320 24.045 445 285 8 137 536 1 437 2.79e-41 161
MS.gene71270.t1 AT2G27110 24.379 443 276 9 132 536 48 469 4.67e-41 161
MS.gene71270.t1 AT2G27110 24.379 443 276 9 132 536 48 469 4.67e-41 161
MS.gene71270.t1 AT4G19990 26.776 366 245 8 171 513 3 368 2.14e-39 155
MS.gene71270.t1 AT2G27110 28.137 263 182 3 279 536 64 324 7.41e-39 154
MS.gene71270.t1 AT4G38170 28.163 245 170 2 298 536 45 289 7.79e-36 143
MS.gene71270.t1 AT5G28530 24.883 426 255 10 139 502 61 483 5.80e-35 142
MS.gene71270.t1 AT5G28530 24.883 426 255 10 139 502 61 483 5.80e-35 142
MS.gene71270.t1 AT5G28530 24.883 426 255 10 139 502 61 483 6.04e-35 142
MS.gene71270.t1 AT5G28530 25.066 379 250 8 139 502 61 420 1.93e-28 121
MS.gene71270.t1 AT5G28530 25.066 379 250 8 139 502 61 420 2.12e-28 121

Find 151 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGCTGCAACACTTATTT+TGG 0.198254 4.2:+64468778 MS.gene71270:CDS
CACAACCAGACAATTATATT+TGG 0.249816 4.2:+64468918 MS.gene71270:CDS
GGTTGCTAGGCATGCGAATT+TGG 0.260542 4.2:+64469892 MS.gene71270:CDS
ACGAAGATGGTTCACATTAC+TGG 0.272975 4.2:+64469858 MS.gene71270:CDS
GCTGCTGCTGTGCTTGTTTA+CGG 0.328972 4.2:+64467353 MS.gene71270:CDS
AATTTGCAAAGGAGTCAATA+AGG 0.330201 4.2:+64469828 MS.gene71270:CDS
ACATTTGTTTGCAATCTAGA+AGG 0.335384 4.2:+64468303 MS.gene71270:CDS
ATTCGAGGAAATTTCTTTGC+AGG 0.336104 4.2:+64469308 MS.gene71270:CDS
TTTGCAATCTAGAAGGGTTC+AGG 0.337269 4.2:+64468310 MS.gene71270:CDS
CCAGAGAATTTACATGTTCC+AGG 0.348297 4.2:+64470037 MS.gene71270:CDS
ATTCTCTGGTCCACCTTCAT+TGG 0.348700 4.2:-64470023 None:intergenic
ATGTTGCAAAGTTGCAATTC+GGG 0.356526 4.2:+64468172 MS.gene71270:CDS
ATCTAACAAATGAAATTAAC+AGG 0.356593 4.2:+64469984 MS.gene71270:CDS
GTGGTTGTTGGAGCAGTTAT+TGG 0.361804 4.2:+64468980 MS.gene71270:CDS
ATGGTGGTGGTAACATAGTT+GGG 0.368143 4.2:+64467791 MS.gene71270:CDS
ACATTACTGGGATTCGCATT+TGG 0.373559 4.2:+64469871 MS.gene71270:CDS
ATTAAGTACTTTGGTAATGC+TGG 0.376669 4.2:+64468549 MS.gene71270:CDS
CATAACCTATGATAACTCTT+TGG 0.380593 4.2:-64469083 None:intergenic
GAAGAGTCGTTTGTAATAGA+TGG 0.385585 4.2:+64468102 MS.gene71270:CDS
TGTCTTAGAATGGAATCTAT+AGG 0.385989 4.2:+64469719 MS.gene71270:intron
CATGAAACCCGTTGCGGTTG+TGG 0.386866 4.2:+64468378 MS.gene71270:CDS
GATGCGTGATTACATCGATA+TGG 0.393225 4.2:-64468416 None:intergenic
TGAAAATGTTGATGACGATT+TGG 0.394802 4.2:+64468029 MS.gene71270:CDS
GCAGCAGCCAGGGGCCTAGC+AGG 0.399566 4.2:-64467338 None:intergenic
CGAAGATGGTTCACATTACT+GGG 0.401396 4.2:+64469859 MS.gene71270:CDS
GATGTTGCAAAGTTGCAATT+CGG 0.402456 4.2:+64468171 MS.gene71270:CDS
TTGTTTCTGCCACTTCCCTT+TGG 0.403113 4.2:+64467303 MS.gene71270:CDS
TCGTAAAGACTCTGTTTGTA+AGG 0.414079 4.2:-64470239 None:intergenic
CAACACTACAGTCCCGTATT+GGG 0.415193 4.2:-64469477 None:intergenic
AAGATTCAAATTAAGTACTT+TGG 0.415197 4.2:+64468540 MS.gene71270:CDS
AAACAAGCACAGCAGCAGCC+AGG 0.417466 4.2:-64467349 None:intergenic
ATGAAAGTGCTTAACAAAAT+CGG 0.425197 4.2:-64469405 None:intergenic
AATGAAATGGTTGAAAGGTT+TGG 0.426607 4.2:+64469221 MS.gene71270:CDS
TCAACACTACAGTCCCGTAT+TGG 0.436480 4.2:-64469478 None:intergenic
ATATTTATTCTTCTTATATG+TGG 0.438535 4.2:-64468859 None:intergenic
ATTTGCAAAGGAGTCAATAA+GGG 0.442855 4.2:+64469829 MS.gene71270:CDS
CCGCAACGGGTTTCATGTAC+TGG 0.444224 4.2:-64468372 None:intergenic
AATGGTGGTGGTAACATAGT+TGG 0.444260 4.2:+64467790 MS.gene71270:CDS
GGGATTCGCATTTGGTTGCT+AGG 0.444717 4.2:+64469879 MS.gene71270:CDS
CATCAACATCCTTGTAAGCT+AGG 0.445568 4.2:-64469940 None:intergenic
TAGTGTTGAAGACCAATCTT+AGG 0.452101 4.2:+64469492 MS.gene71270:CDS
TGTCCTACACTTGATGCAAC+TGG 0.452661 4.2:+64470196 MS.gene71270:CDS
TGCTTGGTGATTATGAAGTC+TGG 0.453304 4.2:+64469180 MS.gene71270:CDS
CATATAAGAAGAATAAATAT+AGG 0.454794 4.2:+64468862 MS.gene71270:CDS
TTTACATGTTCCAGGGGAGT+TGG 0.463269 4.2:+64470045 MS.gene71270:CDS
CCTGGAACATGTAAATTCTC+TGG 0.467772 4.2:-64470037 None:intergenic
GTGGGTTGTTGGCTGCTCAT+AGG 0.473653 4.2:+64468499 MS.gene71270:CDS
CGGTACCAAATATAATTGTC+TGG 0.473826 4.2:-64468923 None:intergenic
AGTTTCTGACCTAGCTTACA+AGG 0.475090 4.2:+64469931 MS.gene71270:CDS
GCCGATCTTAATTCCCAATA+CGG 0.476889 4.2:+64469464 MS.gene71270:CDS
CCTCTCAAGCGACCTAAGAT+TGG 0.477460 4.2:-64469504 None:intergenic
GGTATATGTACGGATTCAAA+TGG 0.478941 4.2:+64467599 MS.gene71270:intron
TTAATGAATTGTATGAGAAA+AGG 0.479026 4.2:+64469264 MS.gene71270:CDS
TGTATGAGAAAAGGAAAATG+TGG 0.479199 4.2:+64469273 MS.gene71270:CDS
AAAGGGTGTGGGCATAGCAC+GGG 0.480342 4.2:+64470100 MS.gene71270:CDS
GATTCAAATGGAAATGATGC+TGG 0.480634 4.2:+64467611 MS.gene71270:CDS
GTTGTTTCAAATGAGGTTGA+AGG 0.481152 4.2:+64468948 MS.gene71270:CDS
GGTAATGCTGGAATCAAAGT+TGG 0.481875 4.2:+64468561 MS.gene71270:CDS
ATTGATGTTTGTTTCACATA+CGG 0.484648 4.2:+64468743 MS.gene71270:CDS
TGGTAACATAGTTGGGGAAG+GGG 0.487877 4.2:+64467798 MS.gene71270:CDS
ACACTAGAAAACAAGACAAA+AGG 0.488324 4.2:-64468888 None:intergenic
AAAGTTGCAATTCGGGAGCC+TGG 0.494683 4.2:+64468179 MS.gene71270:CDS
TTCGAGGAAATTTCTTTGCA+GGG 0.495938 4.2:+64469309 MS.gene71270:CDS
CGAGCATATCATAGCGGTGC+TGG 0.502824 4.2:+64469751 MS.gene71270:CDS
TTTGGAATGAAATGGTTGAA+AGG 0.505319 4.2:+64469216 MS.gene71270:CDS
TGACAAACATTGTGGGTTGT+TGG 0.505785 4.2:+64468488 MS.gene71270:CDS
GATAATGTTGGTGACGGTGA+AGG 0.507211 4.2:+64467820 MS.gene71270:CDS
TGTAGTATATGCGGTGCAGC+AGG 0.512367 4.2:+64470157 MS.gene71270:CDS
ATCAAAGTTGGTCAAATGAT+TGG 0.515889 4.2:+64468573 MS.gene71270:CDS
AGGGACGTACGTGTGGTTGT+TGG 0.515944 4.2:+64468968 MS.gene71270:CDS
AGCTATGAGGAATGCGATCA+AGG 0.519552 4.2:+64469052 MS.gene71270:CDS
GTATGTGAAACAAACATCAA+TGG 0.523303 4.2:-64468741 None:intergenic
ACGATTAAGCAATGACAAGT+AGG 0.526120 4.2:+64470258 MS.gene71270:CDS
GAAATCAAGATTACCAATGT+TGG 0.526439 4.2:-64469135 None:intergenic
AACAAGCACAGCAGCAGCCA+GGG 0.527847 4.2:-64467348 None:intergenic
AATGTTGGTGACGGTGAAGG+AGG 0.528546 4.2:+64467823 MS.gene71270:CDS
CCCGTATTGGGAATTAAGAT+CGG 0.532779 4.2:-64469465 None:intergenic
GTGGTAACATAGTTGGGGAA+GGG 0.533421 4.2:+64467797 MS.gene71270:CDS
CCTTCATGTTCAAGAAGCAA+AGG 0.534160 4.2:+64470082 MS.gene71270:CDS
CAGTTATTGGTTGCAATGAA+AGG 0.534450 4.2:+64468993 MS.gene71270:CDS
ATGATGCTGGAGATAGCGTT+GGG 0.534897 4.2:+64467624 MS.gene71270:CDS
ATAAGGGGAAATTACGAAGA+TGG 0.537087 4.2:+64469845 MS.gene71270:CDS
CCATCTGTTATTACAGCTAC+CGG 0.537754 4.2:-64469023 None:intergenic
CCTTTGCTTCTTGAACATGA+AGG 0.540439 4.2:-64470082 None:intergenic
CAAATATAATTGTCTGGTTG+TGG 0.540768 4.2:-64468917 None:intergenic
CATTTGTTTGCAATCTAGAA+GGG 0.541599 4.2:+64468304 MS.gene71270:CDS
AAATCAAAGTACTCCAATGA+AGG 0.541860 4.2:+64470010 MS.gene71270:CDS
AAAATACAGCCAAAGGGAAG+TGG 0.547215 4.2:-64467312 None:intergenic
TTGTTTCAAATGAGGTTGAA+GGG 0.548979 4.2:+64468949 MS.gene71270:CDS
CCGGTAGCTGTAATAACAGA+TGG 0.555219 4.2:+64469023 MS.gene71270:CDS
TGATGGTAAAGGTATATGTA+CGG 0.558527 4.2:+64467589 MS.gene71270:intron
CAAAGGGTGTGGGCATAGCA+CGG 0.558534 4.2:+64470099 MS.gene71270:CDS
ACAAACGACTCTTCTAGACT+TGG 0.559046 4.2:-64468093 None:intergenic
GTTGGGGAAGGGGATAATGT+TGG 0.562201 4.2:+64467808 MS.gene71270:CDS
CGGTAGCTGTAATAACAGAT+GGG 0.564385 4.2:+64469024 MS.gene71270:CDS
TGTTGGTTTGCAAAGATGAA+CGG 0.565674 4.2:+64468222 MS.gene71270:CDS
AGTCTCTAGTGTTGAATCGT+TGG 0.565920 4.2:+64469801 MS.gene71270:CDS
GGTGCGTTTGCAAATGCTGC+CGG 0.566331 4.2:+64468594 MS.gene71270:CDS
GGTGGTAACATAGTTGGGGA+AGG 0.567379 4.2:+64467796 MS.gene71270:CDS
CTTCATGTTCAAGAAGCAAA+GGG 0.568108 4.2:+64470083 MS.gene71270:CDS
TACCGCTGTTGTTTCAAATG+AGG 0.568284 4.2:+64468941 MS.gene71270:CDS
TGTTCAAGAAGCAAAGGGTG+TGG 0.571034 4.2:+64470088 MS.gene71270:CDS
ACGATTCAACACTAGAGACT+CGG 0.571760 4.2:-64469798 None:intergenic
CGATTCAACACTAGAGACTC+GGG 0.572407 4.2:-64469797 None:intergenic
ACCGCTATGATATGCTCGTA+AGG 0.574356 4.2:-64469746 None:intergenic
CCGATCTTAATTCCCAATAC+GGG 0.578980 4.2:+64469465 MS.gene71270:CDS
GGGTGTGGGCATAGCACGGG+TGG 0.580265 4.2:+64470103 MS.gene71270:CDS
CAGAGAATTTACATGTTCCA+GGG 0.581872 4.2:+64470038 MS.gene71270:CDS
AGTATATTATCCAACTCCCC+TGG 0.581886 4.2:-64470055 None:intergenic
CGAAATGCAATGTCCAACAT+TGG 0.582417 4.2:+64469122 MS.gene71270:CDS
GTTCAAGAAGCAAAGGGTGT+GGG 0.583267 4.2:+64470089 MS.gene71270:CDS
AATGTTGATGACGATTTGGA+AGG 0.584634 4.2:+64468033 MS.gene71270:CDS
GTATGAGAAAAGGAAAATGT+GGG 0.585286 4.2:+64469274 MS.gene71270:CDS
AATGATGCTGGAGATAGCGT+TGG 0.586809 4.2:+64467623 MS.gene71270:CDS
GCATAGCACGGGTGGTACAA+TGG 0.586809 4.2:+64470111 MS.gene71270:CDS
GATGAGTTAACACACGAGAA+TGG 0.588219 4.2:+64467772 MS.gene71270:CDS
ATACGGTTGATGCGGAAGGT+AGG 0.588882 4.2:+64468760 MS.gene71270:CDS
TCGATGTAATCACGCATCGT+TGG 0.589562 4.2:+64468421 MS.gene71270:CDS
TCACATACGGTTGATGCGGA+AGG 0.596764 4.2:+64468756 MS.gene71270:CDS
TGGGCAACAAGTCATATTCG+AGG 0.597756 4.2:+64469293 MS.gene71270:CDS
CAGATGGGGATCTAGCTATG+AGG 0.599944 4.2:+64469039 MS.gene71270:CDS
TGGTGGTGGTAACATAGTTG+GGG 0.601559 4.2:+64467792 MS.gene71270:CDS
CCAATCTTAGGTCGCTTGAG+AGG 0.601635 4.2:+64469504 MS.gene71270:CDS
TCAAAGTACTCCAATGAAGG+TGG 0.603482 4.2:+64470013 MS.gene71270:CDS
GAATTTGGTTTATCTTAGCA+AGG 0.606337 4.2:+64469907 MS.gene71270:CDS
GTTATCATAGGTTATGTGCA+TGG 0.608973 4.2:+64469090 MS.gene71270:CDS
TCGTTGGTCACAATTTGCAA+AGG 0.617164 4.2:+64469817 MS.gene71270:CDS
GAAGGGGATAATGTTGGTGA+CGG 0.619105 4.2:+64467814 MS.gene71270:CDS
TGTTGAGTGACAAACATTGT+GGG 0.620764 4.2:+64468481 MS.gene71270:CDS
TCTCCAGTTGCATCAAGTGT+AGG 0.620920 4.2:-64470199 None:intergenic
TGTTTCACATACGGTTGATG+CGG 0.624870 4.2:+64468752 MS.gene71270:CDS
TTAACACACGAGAATGGTGG+TGG 0.625361 4.2:+64467778 MS.gene71270:CDS
TTTGCAAAGGAGTCAATAAG+GGG 0.629931 4.2:+64469830 MS.gene71270:CDS
AGACAGAGGTCTAACAGCAG+AGG 0.634039 4.2:+64468335 MS.gene71270:CDS
CAGGGATAAGAACAACATCA+AGG 0.636558 4.2:+64469327 MS.gene71270:CDS
ATGTTGAGTGACAAACATTG+TGG 0.650930 4.2:+64468480 MS.gene71270:CDS
ACAGCAGAGGAGCGTAAGCG+TGG 0.663483 4.2:+64468348 MS.gene71270:CDS
AGAGAATTTACATGTTCCAG+GGG 0.665809 4.2:+64470039 MS.gene71270:CDS
TGCAATGAAAGGTAAGACAC+CGG 0.669695 4.2:+64469004 MS.gene71270:CDS
AAGAACAACATCAAGGTGTG+AGG 0.673503 4.2:+64469334 MS.gene71270:CDS
ACAAGCACAGCAGCAGCCAG+GGG 0.673883 4.2:-64467347 None:intergenic
GAAGGGTTCAGGAAAGACAG+AGG 0.686044 4.2:+64468321 MS.gene71270:CDS
ACACAGACTTGTAGTATATG+CGG 0.687479 4.2:+64470148 MS.gene71270:CDS
GGTAGCTGTAATAACAGATG+GGG 0.691783 4.2:+64469025 MS.gene71270:CDS
GAGTTAACACACGAGAATGG+TGG 0.694624 4.2:+64467775 MS.gene71270:CDS
AGGTTGAAGGGACGTACGTG+TGG 0.703584 4.2:+64468961 MS.gene71270:CDS
GTACAATGGAAAGACGTCGA+CGG 0.704653 4.2:+64470125 MS.gene71270:CDS
AACCTCATTTGAAACAACAG+CGG 0.707858 4.2:-64468943 None:intergenic
CCAGTACATGAAACCCGTTG+CGG 0.717976 4.2:+64468372 MS.gene71270:CDS
TTCTGTAGAATGAATCACAG+AGG 0.744987 4.2:+64467126 None:intergenic
TCCTTACGAGCATATCATAG+CGG 0.756507 4.2:+64469745 MS.gene71270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATAATAAAAAAAAAT+AGG - chr4.2:64467715-64467734 None:intergenic 0.0%
!! TGATGATTATAAAAATTATT+TGG + chr4.2:64469958-64469977 MS.gene71270:CDS 10.0%
!! ATATTTATTCTTCTTATATG+TGG - chr4.2:64468862-64468881 None:intergenic 15.0%
!! CAAGTTATTAAAAATAAGAA+CGG + chr4.2:64468264-64468283 MS.gene71270:CDS 15.0%
!! CATATAAGAAGAATAAATAT+AGG + chr4.2:64468862-64468881 MS.gene71270:CDS 15.0%
!! TAGAAATTGTATAAATTTGA+TGG + chr4.2:64467572-64467591 MS.gene71270:intron 15.0%
!!! GAAGTGAATTTTTTTTTAAA+CGG - chr4.2:64467522-64467541 None:intergenic 15.0%
!! AAACCAACAATAAAAATTGT+AGG - chr4.2:64468211-64468230 None:intergenic 20.0%
!! AAGATTCAAATTAAGTACTT+TGG + chr4.2:64468540-64468559 MS.gene71270:CDS 20.0%
!! ACAAAGATATCTATAATTTG+TGG + chr4.2:64467683-64467702 MS.gene71270:intron 20.0%
!! ATCTAACAAATGAAATTAAC+AGG + chr4.2:64469984-64470003 MS.gene71270:CDS 20.0%
!! TTAATGAATTGTATGAGAAA+AGG + chr4.2:64469264-64469283 MS.gene71270:CDS 20.0%
!!! CAAAAGAATTTTGAAGTTTT+TGG + chr4.2:64468816-64468835 MS.gene71270:CDS 20.0%
!!! CATGTATAGCTTATTTTTTT+TGG + chr4.2:64467270-64467289 MS.gene71270:intron 20.0%
!!! TTGTATAAATTTGATGGTAA+AGG + chr4.2:64467578-64467597 MS.gene71270:intron 20.0%
! ATTGATGTTTGTTTCACATA+CGG + chr4.2:64468743-64468762 MS.gene71270:CDS 25.0%
! TACCATCTCAAAAATTTCTT+TGG - chr4.2:64469549-64469568 None:intergenic 25.0%
! TCTGGAAATTTGAAAAACTT+TGG + chr4.2:64469198-64469217 MS.gene71270:CDS 25.0%
!! ATTTTGTTAAGCACTTTCAT+AGG + chr4.2:64469408-64469427 MS.gene71270:CDS 25.0%
!! TAGCATTTATTCACTGTTTT+TGG + chr4.2:64467165-64467184 MS.gene71270:intron 25.0%
!! TGTAGAACAAGACATATTTT+GGG - chr4.2:64469615-64469634 None:intergenic 25.0%
!! TTGCCTACAATTTTTATTGT+TGG + chr4.2:64468205-64468224 MS.gene71270:CDS 25.0%
!! TTGTAGAACAAGACATATTT+TGG - chr4.2:64469616-64469635 None:intergenic 25.0%
!! TTTTTCCAAAGAGTTATCAT+AGG + chr4.2:64469078-64469097 MS.gene71270:CDS 25.0%
!!! AGCATACATCTTTTGAAAAA+AGG - chr4.2:64469164-64469183 None:intergenic 25.0%
!!! ATGAAAGTGCTTAACAAAAT+CGG - chr4.2:64469408-64469427 None:intergenic 25.0%
!!! ATGTTTTTTTTAGCAAGCAA+TGG + chr4.2:64467750-64467769 MS.gene71270:intron 25.0%
!!! GCATACATCTTTTGAAAAAA+GGG - chr4.2:64469163-64469182 None:intergenic 25.0%
!!! GCTTATTTTTTTTGGTGTTT+AGG + chr4.2:64467278-64467297 MS.gene71270:intron 25.0%
!!! TACCAAAGAAATTTTTGAGA+TGG + chr4.2:64469544-64469563 MS.gene71270:intron 25.0%
!!! TGAAAAACTTTGGAATGAAA+TGG + chr4.2:64469208-64469227 MS.gene71270:CDS 25.0%
!!! TTTTTCAAAAGATGTATGCT+TGG + chr4.2:64469164-64469183 MS.gene71270:CDS 25.0%
AATGAAATGGTTGAAAGGTT+TGG + chr4.2:64469221-64469240 MS.gene71270:CDS 30.0%
AATGGAAATTCTGATTCAGA+GGG + chr4.2:64467997-64468016 MS.gene71270:intron 30.0%
AATTTGCAAAGGAGTCAATA+AGG + chr4.2:64469828-64469847 MS.gene71270:CDS 30.0%
ACACTAGAAAACAAGACAAA+AGG - chr4.2:64468891-64468910 None:intergenic 30.0%
ACATTTGTTTGCAATCTAGA+AGG + chr4.2:64468303-64468322 MS.gene71270:CDS 30.0%
AGGAAAAAAATACAGCCAAA+GGG - chr4.2:64467321-64467340 None:intergenic 30.0%
ATTTGCAAAGGAGTCAATAA+GGG + chr4.2:64469829-64469848 MS.gene71270:CDS 30.0%
CAAATATAATTGTCTGGTTG+TGG - chr4.2:64468920-64468939 None:intergenic 30.0%
CACAACCAGACAATTATATT+TGG + chr4.2:64468918-64468937 MS.gene71270:CDS 30.0%
CATAACCTATGATAACTCTT+TGG - chr4.2:64469086-64469105 None:intergenic 30.0%
CATTTGTTTGCAATCTAGAA+GGG + chr4.2:64468304-64468323 MS.gene71270:CDS 30.0%
GAAATCAAGATTACCAATGT+TGG - chr4.2:64469138-64469157 None:intergenic 30.0%
GATGTAAATGAAGATGATTC+TGG + chr4.2:64467943-64467962 MS.gene71270:intron 30.0%
GTATGAGAAAAGGAAAATGT+GGG + chr4.2:64469274-64469293 MS.gene71270:CDS 30.0%
GTATGTGAAACAAACATCAA+TGG - chr4.2:64468744-64468763 None:intergenic 30.0%
TAAACAAAAACCTGCTACAT+CGG - chr4.2:64467227-64467246 None:intergenic 30.0%
TGTATGAGAAAAGGAAAATG+TGG + chr4.2:64469273-64469292 MS.gene71270:CDS 30.0%
TGTCTTAGAATGGAATCTAT+AGG + chr4.2:64469719-64469738 MS.gene71270:intron 30.0%
TTGTTTCAAATGAGGTTGAA+GGG + chr4.2:64468949-64468968 MS.gene71270:CDS 30.0%
! AAATCAAAGTACTCCAATGA+AGG + chr4.2:64470010-64470029 MS.gene71270:CDS 30.0%
! ATCAAAGTTGGTCAAATGAT+TGG + chr4.2:64468573-64468592 MS.gene71270:CDS 30.0%
! GAATTTGGTTTATCTTAGCA+AGG + chr4.2:64469907-64469926 MS.gene71270:CDS 30.0%
! GATGTGAATGATGATGATTA+TGG + chr4.2:64467919-64467938 MS.gene71270:intron 30.0%
! TGATGGTAAAGGTATATGTA+CGG + chr4.2:64467589-64467608 MS.gene71270:intron 30.0%
! TTCTTTGGTAAACTGTTTTG+AGG - chr4.2:64469534-64469553 None:intergenic 30.0%
! TTTGGAATGAAATGGTTGAA+AGG + chr4.2:64469216-64469235 MS.gene71270:CDS 30.0%
!! ATTAAGTACTTTGGTAATGC+TGG + chr4.2:64468549-64468568 MS.gene71270:CDS 30.0%
!! CAGGTTTTTGTTTATTGGTT+TGG + chr4.2:64467233-64467252 MS.gene71270:intron 30.0%
!! GTTTATTGGTTTGGCTTTAA+GGG + chr4.2:64467242-64467261 MS.gene71270:intron 30.0%
!! TGAAAATGTTGATGACGATT+TGG + chr4.2:64468029-64468048 MS.gene71270:CDS 30.0%
!! TGTAGCAGGTTTTTGTTTAT+TGG + chr4.2:64467228-64467247 MS.gene71270:intron 30.0%
!! TGTTTATTGGTTTGGCTTTA+AGG + chr4.2:64467241-64467260 MS.gene71270:intron 30.0%
!!! ACTGTTTTTGGATCTACTTT+GGG + chr4.2:64467177-64467196 MS.gene71270:intron 30.0%
!!! TGCATATTTTCGCTTTAAAG+AGG + chr4.2:64469436-64469455 MS.gene71270:CDS 30.0%
!!! TTGGTTTTGAAGAAAACAGA+TGG + chr4.2:64469240-64469259 MS.gene71270:CDS 30.0%
AAAAATTGTAGGCAACTTCC+AGG - chr4.2:64468200-64468219 None:intergenic 35.0%
ACACAGACTTGTAGTATATG+CGG + chr4.2:64470148-64470167 MS.gene71270:CDS 35.0%
AGAATTTCCATTCACATCAC+CGG - chr4.2:64467989-64468008 None:intergenic 35.0%
AGAGAATTTACATGTTCCAG+GGG + chr4.2:64470039-64470058 MS.gene71270:CDS 35.0%
ATAAGGGGAAATTACGAAGA+TGG + chr4.2:64469845-64469864 MS.gene71270:CDS 35.0%
ATCATCATCATTCACATCAC+CGG - chr4.2:64467917-64467936 None:intergenic 35.0%
ATGTTGAGTGACAAACATTG+TGG + chr4.2:64468480-64468499 MS.gene71270:CDS 35.0%
ATGTTGCAAAGTTGCAATTC+GGG + chr4.2:64468172-64468191 MS.gene71270:CDS 35.0%
ATTCGAGGAAATTTCTTTGC+AGG + chr4.2:64469308-64469327 MS.gene71270:CDS 35.0%
CAGAGAATTTACATGTTCCA+GGG + chr4.2:64470038-64470057 MS.gene71270:CDS 35.0%
CAGGAAAAAAATACAGCCAA+AGG - chr4.2:64467322-64467341 None:intergenic 35.0%
CATGCAAATTGATTGTCCAA+TGG - chr4.2:64469695-64469714 None:intergenic 35.0%
CGGTACCAAATATAATTGTC+TGG - chr4.2:64468926-64468945 None:intergenic 35.0%
CTTCATGTTCAAGAAGCAAA+GGG + chr4.2:64470083-64470102 MS.gene71270:CDS 35.0%
GAAGAGTCGTTTGTAATAGA+TGG + chr4.2:64468102-64468121 MS.gene71270:CDS 35.0%
GAATGGAAATTCTGATTCAG+AGG + chr4.2:64467996-64468015 MS.gene71270:intron 35.0%
GATGTTGCAAAGTTGCAATT+CGG + chr4.2:64468171-64468190 MS.gene71270:CDS 35.0%
GATTCAAATGGAAATGATGC+TGG + chr4.2:64467611-64467630 MS.gene71270:CDS 35.0%
GGTATATGTACGGATTCAAA+TGG + chr4.2:64467599-64467618 MS.gene71270:intron 35.0%
GGTGTCTAACAAAGATACTA+GGG - chr4.2:64469594-64469613 None:intergenic 35.0%
GTTATCATAGGTTATGTGCA+TGG + chr4.2:64469090-64469109 MS.gene71270:CDS 35.0%
TACGAAGATGAAGATGATTC+CGG + chr4.2:64467895-64467914 MS.gene71270:intron 35.0%
TCGTAAAGACTCTGTTTGTA+AGG - chr4.2:64470242-64470261 None:intergenic 35.0%
TGCATGTTCTTGTCTTAGAA+TGG + chr4.2:64469709-64469728 MS.gene71270:intron 35.0%
TGTTGAGTGACAAACATTGT+GGG + chr4.2:64468481-64468500 MS.gene71270:CDS 35.0%
TTCGAGGAAATTTCTTTGCA+GGG + chr4.2:64469309-64469328 MS.gene71270:CDS 35.0%
TTTGCAAAGGAGTCAATAAG+GGG + chr4.2:64469830-64469849 MS.gene71270:CDS 35.0%
! AACCTCATTTGAAACAACAG+CGG - chr4.2:64468946-64468965 None:intergenic 35.0%
! AAGATACTAGGGATACCTTT+TGG - chr4.2:64469583-64469602 None:intergenic 35.0%
! CAGTTATTGGTTGCAATGAA+AGG + chr4.2:64468993-64469012 MS.gene71270:CDS 35.0%
! GATGTGAATGATGATGATTC+CGG + chr4.2:64467967-64467986 MS.gene71270:intron 35.0%
! GTTGTTTCAAATGAGGTTGA+AGG + chr4.2:64468948-64468967 MS.gene71270:CDS 35.0%
! TAAGGTAGGATTTCTGAAGA+AGG + chr4.2:64468626-64468645 MS.gene71270:intron 35.0%
! TAGTGTTGAAGACCAATCTT+AGG + chr4.2:64469492-64469511 MS.gene71270:CDS 35.0%
!! AATGTTGATGACGATTTGGA+AGG + chr4.2:64468033-64468052 MS.gene71270:CDS 35.0%
!! TGTTGGTTTGCAAAGATGAA+CGG + chr4.2:64468222-64468241 MS.gene71270:CDS 35.0%
!! TTGACAAAGTATCGAGATGA+AGG + chr4.2:64469644-64469663 MS.gene71270:intron 35.0%
!!! CACTGTTTTTGGATCTACTT+TGG + chr4.2:64467176-64467195 MS.gene71270:intron 35.0%
!!! CTGTTTTTGGATCTACTTTG+GGG + chr4.2:64467178-64467197 MS.gene71270:intron 35.0%
!!! TGTTTTTGGATCTACTTTGG+GGG + chr4.2:64467179-64467198 MS.gene71270:intron 35.0%
AAAATACAGCCAAAGGGAAG+TGG - chr4.2:64467315-64467334 None:intergenic 40.0%
AAGAACAACATCAAGGTGTG+AGG + chr4.2:64469334-64469353 MS.gene71270:CDS 40.0%
ACAAACGACTCTTCTAGACT+TGG - chr4.2:64468096-64468115 None:intergenic 40.0%
ACATTACTGGGATTCGCATT+TGG + chr4.2:64469871-64469890 MS.gene71270:CDS 40.0%
ACGAAGATGGTTCACATTAC+TGG + chr4.2:64469858-64469877 MS.gene71270:CDS 40.0%
ACGATTCAACACTAGAGACT+CGG - chr4.2:64469801-64469820 None:intergenic 40.0%
AGTATATTATCCAACTCCCC+TGG - chr4.2:64470058-64470077 None:intergenic 40.0%
AGTTTCTGACCTAGCTTACA+AGG + chr4.2:64469931-64469950 MS.gene71270:CDS 40.0%
ATCGAGATGAAGGTTATGTC+TGG + chr4.2:64469654-64469673 MS.gene71270:intron 40.0%
CAGGGATAAGAACAACATCA+AGG + chr4.2:64469327-64469346 MS.gene71270:CDS 40.0%
CATCAACATCCTTGTAAGCT+AGG - chr4.2:64469943-64469962 None:intergenic 40.0%
CCAGAGAATTTACATGTTCC+AGG + chr4.2:64470037-64470056 MS.gene71270:CDS 40.0%
CCATCTGTTATTACAGCTAC+CGG - chr4.2:64469026-64469045 None:intergenic 40.0%
CCCGTATTGGGAATTAAGAT+CGG - chr4.2:64469468-64469487 None:intergenic 40.0%
CCGATCTTAATTCCCAATAC+GGG + chr4.2:64469465-64469484 MS.gene71270:CDS 40.0%
CCTGGAACATGTAAATTCTC+TGG - chr4.2:64470040-64470059 None:intergenic 40.0%
CCTTCATGTTCAAGAAGCAA+AGG + chr4.2:64470082-64470101 MS.gene71270:CDS 40.0%
CCTTTGCTTCTTGAACATGA+AGG - chr4.2:64470085-64470104 None:intergenic 40.0%
CGAAATGCAATGTCCAACAT+TGG + chr4.2:64469122-64469141 MS.gene71270:CDS 40.0%
CGAAGATGGTTCACATTACT+GGG + chr4.2:64469859-64469878 MS.gene71270:CDS 40.0%
CGGTAGCTGTAATAACAGAT+GGG + chr4.2:64469024-64469043 MS.gene71270:CDS 40.0%
GATGAGTTAACACACGAGAA+TGG + chr4.2:64467772-64467791 MS.gene71270:CDS 40.0%
GATGCGTGATTACATCGATA+TGG - chr4.2:64468419-64468438 None:intergenic 40.0%
GCCGATCTTAATTCCCAATA+CGG + chr4.2:64469464-64469483 MS.gene71270:CDS 40.0%
GGGTGTCTAACAAAGATACT+AGG - chr4.2:64469595-64469614 None:intergenic 40.0%
GGTAATGCTGGAATCAAAGT+TGG + chr4.2:64468561-64468580 MS.gene71270:CDS 40.0%
GGTAGCTGTAATAACAGATG+GGG + chr4.2:64469025-64469044 MS.gene71270:CDS 40.0%
TCCTTACGAGCATATCATAG+CGG + chr4.2:64469745-64469764 MS.gene71270:CDS 40.0%
TCGATGTCATCTGATGCTAA+AGG + chr4.2:64468681-64468700 MS.gene71270:intron 40.0%
TCGTTGGTCACAATTTGCAA+AGG + chr4.2:64469817-64469836 MS.gene71270:CDS 40.0%
TGCAATGAAAGGTAAGACAC+CGG + chr4.2:64469004-64469023 MS.gene71270:CDS 40.0%
TTTGCAATCTAGAAGGGTTC+AGG + chr4.2:64468310-64468329 MS.gene71270:CDS 40.0%
! AGTCTCTAGTGTTGAATCGT+TGG + chr4.2:64469801-64469820 MS.gene71270:CDS 40.0%
! ATTTTTCTCCACAACCGCAA+CGG - chr4.2:64468389-64468408 None:intergenic 40.0%
! GCTTTTTGTGTTGTATCAGC+TGG + chr4.2:64467385-64467404 MS.gene71270:intron 40.0%
! GTAGGCTGCAACACTTATTT+TGG + chr4.2:64468778-64468797 MS.gene71270:CDS 40.0%
! TCAAAGTACTCCAATGAAGG+TGG + chr4.2:64470013-64470032 MS.gene71270:CDS 40.0%
! TGACAAACATTGTGGGTTGT+TGG + chr4.2:64468488-64468507 MS.gene71270:CDS 40.0%
! TGCTTGGTGATTATGAAGTC+TGG + chr4.2:64469180-64469199 MS.gene71270:CDS 40.0%
! TGTTTCACATACGGTTGATG+CGG + chr4.2:64468752-64468771 MS.gene71270:CDS 40.0%
!! AACGGTTTTGCTGTCAGAAA+AGG + chr4.2:64468240-64468259 MS.gene71270:CDS 40.0%
!! AATGGTGGTGGTAACATAGT+TGG + chr4.2:64467790-64467809 MS.gene71270:CDS 40.0%
!! ATGGTGGTGGTAACATAGTT+GGG + chr4.2:64467791-64467810 MS.gene71270:CDS 40.0%
!! ATTGGTTTGGCTTTAAGGGA+GGG + chr4.2:64467246-64467265 MS.gene71270:intron 40.0%
!! TACCGCTGTTGTTTCAAATG+AGG + chr4.2:64468941-64468960 MS.gene71270:CDS 40.0%
!! TATTGGTTTGGCTTTAAGGG+AGG + chr4.2:64467245-64467264 MS.gene71270:intron 40.0%
!!! CAACACTTATTTTGGAGCGA+CGG + chr4.2:64468786-64468805 MS.gene71270:CDS 40.0%
!!! GGCTGTATTTTTTTCCTGCT+AGG + chr4.2:64467324-64467343 MS.gene71270:CDS 40.0%
AATGATGCTGGAGATAGCGT+TGG + chr4.2:64467623-64467642 MS.gene71270:CDS 45.0%
ACCGCTATGATATGCTCGTA+AGG - chr4.2:64469749-64469768 None:intergenic 45.0%
AGCTATGAGGAATGCGATCA+AGG + chr4.2:64469052-64469071 MS.gene71270:CDS 45.0%
ATGATGCTGGAGATAGCGTT+GGG + chr4.2:64467624-64467643 MS.gene71270:CDS 45.0%
ATTCTCTGGTCCACCTTCAT+TGG - chr4.2:64470026-64470045 None:intergenic 45.0%
CAACACTACAGTCCCGTATT+GGG - chr4.2:64469480-64469499 None:intergenic 45.0%
CCGGTAGCTGTAATAACAGA+TGG + chr4.2:64469023-64469042 MS.gene71270:CDS 45.0%
CGATTCAACACTAGAGACTC+GGG - chr4.2:64469800-64469819 None:intergenic 45.0%
GAGTTAACACACGAGAATGG+TGG + chr4.2:64467775-64467794 MS.gene71270:CDS 45.0%
GATGATTCCGGTGATGTGAA+TGG + chr4.2:64467907-64467926 MS.gene71270:intron 45.0%
GGTACGATCTGTCATCCAAA+AGG + chr4.2:64469565-64469584 MS.gene71270:intron 45.0%
GTACAATGGAAAGACGTCGA+CGG + chr4.2:64470125-64470144 MS.gene71270:CDS 45.0%
GTCTGTTTCGCATAGTCCAT+TGG + chr4.2:64469676-64469695 MS.gene71270:intron 45.0%
GTGCTTGTTTACGGTTAGCA+GGG + chr4.2:64467362-64467381 MS.gene71270:intron 45.0%
GTGGTAACATAGTTGGGGAA+GGG + chr4.2:64467797-64467816 MS.gene71270:CDS 45.0%
GTGGTTGTTGGAGCAGTTAT+TGG + chr4.2:64468980-64468999 MS.gene71270:CDS 45.0%
TCAACACTACAGTCCCGTAT+TGG - chr4.2:64469481-64469500 None:intergenic 45.0%
TCCTACCTTATCATACCCAC+CGG - chr4.2:64468616-64468635 None:intergenic 45.0%
TCGATGTAATCACGCATCGT+TGG + chr4.2:64468421-64468440 MS.gene71270:CDS 45.0%
TCTCCAGTTGCATCAAGTGT+AGG - chr4.2:64470202-64470221 None:intergenic 45.0%
TGCTTGTTTACGGTTAGCAG+GGG + chr4.2:64467363-64467382 MS.gene71270:intron 45.0%
TGGGCAACAAGTCATATTCG+AGG + chr4.2:64469293-64469312 MS.gene71270:CDS 45.0%
TGGTAACATAGTTGGGGAAG+GGG + chr4.2:64467798-64467817 MS.gene71270:CDS 45.0%
TGTCCTACACTTGATGCAAC+TGG + chr4.2:64470196-64470215 MS.gene71270:CDS 45.0%
TGTGCTTGTTTACGGTTAGC+AGG + chr4.2:64467361-64467380 MS.gene71270:intron 45.0%
TGTTCAAGAAGCAAAGGGTG+TGG + chr4.2:64470088-64470107 MS.gene71270:CDS 45.0%
TTAACACACGAGAATGGTGG+TGG + chr4.2:64467778-64467797 MS.gene71270:CDS 45.0%
TTTACATGTTCCAGGGGAGT+TGG + chr4.2:64470045-64470064 MS.gene71270:CDS 45.0%
! CAAGCACCAGAAGTGCAAAA+AGG - chr4.2:64467411-64467430 None:intergenic 45.0%
! CTTTTTGCACTTCTGGTGCT+TGG + chr4.2:64467409-64467428 MS.gene71270:intron 45.0%
! GAAGGGGATAATGTTGGTGA+CGG + chr4.2:64467814-64467833 MS.gene71270:CDS 45.0%
! GTTCAAGAAGCAAAGGGTGT+GGG + chr4.2:64470089-64470108 MS.gene71270:CDS 45.0%
! TTTTTCTCCACAACCGCAAC+GGG - chr4.2:64468388-64468407 None:intergenic 45.0%
!! GATAATGTTGGTGACGGTGA+AGG + chr4.2:64467820-64467839 MS.gene71270:CDS 45.0%
!! TGGTGGTGGTAACATAGTTG+GGG + chr4.2:64467792-64467811 MS.gene71270:CDS 45.0%
!! TTGTTTCTGCCACTTCCCTT+TGG + chr4.2:64467303-64467322 MS.gene71270:CDS 45.0%
!! TATATATATATATATATAAG+AGG - chr4.2:64467435-64467454 None:intergenic 5.0%
AAAGTTGCAATTCGGGAGCC+TGG + chr4.2:64468179-64468198 MS.gene71270:CDS 50.0%
AGACAGAGGTCTAACAGCAG+AGG + chr4.2:64468335-64468354 MS.gene71270:CDS 50.0%
CAGATGGGGATCTAGCTATG+AGG + chr4.2:64469039-64469058 MS.gene71270:CDS 50.0%
CCAATCTTAGGTCGCTTGAG+AGG + chr4.2:64469504-64469523 MS.gene71270:CDS 50.0%
CCAGTACATGAAACCCGTTG+CGG + chr4.2:64468372-64468391 MS.gene71270:CDS 50.0%
CCTCTCAAGCGACCTAAGAT+TGG - chr4.2:64469507-64469526 None:intergenic 50.0%
GAAGGGTTCAGGAAAGACAG+AGG + chr4.2:64468321-64468340 MS.gene71270:CDS 50.0%
GCTGCTGCTGTGCTTGTTTA+CGG + chr4.2:64467353-64467372 MS.gene71270:CDS 50.0%
GGGATTCGCATTTGGTTGCT+AGG + chr4.2:64469879-64469898 MS.gene71270:CDS 50.0%
GGTGGTAACATAGTTGGGGA+AGG + chr4.2:64467796-64467815 MS.gene71270:CDS 50.0%
GGTTGCTAGGCATGCGAATT+TGG + chr4.2:64469892-64469911 MS.gene71270:CDS 50.0%
GTTGGGGAAGGGGATAATGT+TGG + chr4.2:64467808-64467827 MS.gene71270:CDS 50.0%
TGTAGTATATGCGGTGCAGC+AGG + chr4.2:64470157-64470176 MS.gene71270:CDS 50.0%
! ATACGGTTGATGCGGAAGGT+AGG + chr4.2:64468760-64468779 MS.gene71270:CDS 50.0%
! ATCTACTTTGGGGGTCTGTG+AGG + chr4.2:64467188-64467207 MS.gene71270:intron 50.0%
! TCACATACGGTTGATGCGGA+AGG + chr4.2:64468756-64468775 MS.gene71270:CDS 50.0%
!! AATGTTGGTGACGGTGAAGG+AGG + chr4.2:64467823-64467842 MS.gene71270:CDS 50.0%
!!! AGCTGGCCTTTTTGCACTTC+TGG + chr4.2:64467402-64467421 MS.gene71270:intron 50.0%
!!! TTTTTTTCCTGCTAGGCCCC+TGG + chr4.2:64467331-64467350 MS.gene71270:CDS 50.0%
AAACAAGCACAGCAGCAGCC+AGG - chr4.2:64467352-64467371 None:intergenic 55.0%
AACAAGCACAGCAGCAGCCA+GGG - chr4.2:64467351-64467370 None:intergenic 55.0%
AGGGACGTACGTGTGGTTGT+TGG + chr4.2:64468968-64468987 MS.gene71270:CDS 55.0%
AGGTTGAAGGGACGTACGTG+TGG + chr4.2:64468961-64468980 MS.gene71270:CDS 55.0%
CATGAAACCCGTTGCGGTTG+TGG + chr4.2:64468378-64468397 MS.gene71270:CDS 55.0%
CGAGCATATCATAGCGGTGC+TGG + chr4.2:64469751-64469770 MS.gene71270:CDS 55.0%
CGTTTGCAAATGCTGCCGGT+GGG + chr4.2:64468598-64468617 MS.gene71270:intron 55.0%
GCATAGCACGGGTGGTACAA+TGG + chr4.2:64470111-64470130 MS.gene71270:CDS 55.0%
GCCGGTGGGTATGATAAGGT+AGG + chr4.2:64468612-64468631 MS.gene71270:intron 55.0%
GGTGCGTTTGCAAATGCTGC+CGG + chr4.2:64468594-64468613 MS.gene71270:CDS 55.0%
TGCTGCCGGTGGGTATGATA+AGG + chr4.2:64468608-64468627 MS.gene71270:intron 55.0%
! AAAGGGTGTGGGCATAGCAC+GGG + chr4.2:64470100-64470119 MS.gene71270:CDS 55.0%
! CAAAGGGTGTGGGCATAGCA+CGG + chr4.2:64470099-64470118 MS.gene71270:CDS 55.0%
! CCGCAACGGGTTTCATGTAC+TGG - chr4.2:64468375-64468394 None:intergenic 55.0%
! CTTTGGGGGTCTGTGAGGTT+CGG + chr4.2:64467193-64467212 MS.gene71270:intron 55.0%
! GTGGGTTGTTGGCTGCTCAT+AGG + chr4.2:64468499-64468518 MS.gene71270:CDS 55.0%
ACAAGCACAGCAGCAGCCAG+GGG - chr4.2:64467350-64467369 None:intergenic 60.0%
GCGTTTGCAAATGCTGCCGG+TGG + chr4.2:64468597-64468616 MS.gene71270:intron 60.0%
GGCACTTCAGCCGATGTAGC+AGG + chr4.2:64467214-64467233 MS.gene71270:intron 60.0%
! ACAGCAGAGGAGCGTAAGCG+TGG + chr4.2:64468348-64468367 MS.gene71270:CDS 60.0%
GGGTGTGGGCATAGCACGGG+TGG + chr4.2:64470103-64470122 MS.gene71270:CDS 70.0%
GCAGCAGCCAGGGGCCTAGC+AGG - chr4.2:64467341-64467360 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 64467135 64470279 64467135 ID=MS.gene71270
chr4.2 mRNA 64467135 64470279 64467135 ID=MS.gene71270.t1;Parent=MS.gene71270
chr4.2 exon 64467135 64467147 64467135 ID=MS.gene71270.t1.exon1;Parent=MS.gene71270.t1
chr4.2 CDS 64467135 64467147 64467135 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64467300 64467374 64467300 ID=MS.gene71270.t1.exon2;Parent=MS.gene71270.t1
chr4.2 CDS 64467300 64467374 64467300 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64467600 64467670 64467600 ID=MS.gene71270.t1.exon3;Parent=MS.gene71270.t1
chr4.2 CDS 64467600 64467670 64467600 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64467763 64467844 64467763 ID=MS.gene71270.t1.exon4;Parent=MS.gene71270.t1
chr4.2 CDS 64467763 64467844 64467763 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64468016 64468615 64468016 ID=MS.gene71270.t1.exon5;Parent=MS.gene71270.t1
chr4.2 CDS 64468016 64468615 64468016 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64468739 64469525 64468739 ID=MS.gene71270.t1.exon6;Parent=MS.gene71270.t1
chr4.2 CDS 64468739 64469525 64468739 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
chr4.2 exon 64469727 64470279 64469727 ID=MS.gene71270.t1.exon7;Parent=MS.gene71270.t1
chr4.2 CDS 64469727 64470279 64469727 ID=cds.MS.gene71270.t1;Parent=MS.gene71270.t1
Gene Sequence

>MS.gene71270

ATGAATCACAGAGGTAAGTTCTTAGTTATTTAGCATTTATTCACTGTTTTTGGATCTACTTTGGGGGTCTGTGAGGTTCGGCACTTCAGCCGATGTAGCAGGTTTTTGTTTATTGGTTTGGCTTTAAGGGAGGGTCATGTATAGCTTATTTTTTTTGGTGTTTAGGCTTTGTTTCTGCCACTTCCCTTTGGCTGTATTTTTTTCCTGCTAGGCCCCTGGCTGCTGCTGTGCTTGTTTACGGTTAGCAGGGGCTTTTTGTGTTGTATCAGCTGGCCTTTTTGCACTTCTGGTGCTTGGCCTCTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGCCGTTTAAAAAAAAATTCACTTCTTAATTATTTAAGTCTGAAGTAAAATTTAATAGAAATTGTATAAATTTGATGGTAAAGGTATATGTACGGATTCAAATGGAAATGATGCTGGAGATAGCGTTGGGCATGAAGTAATGCAAGCTTATTATGTAAGTGAAAATACAAAGATATCTATAATTTGTGGTTGTTACCTATTTTTTTTTATTATTATTTGTCTCAGTTATGTAAATGTTTTTTTTAGCAAGCAATGGATGAGTTAACACACGAGAATGGTGGTGGTAACATAGTTGGGGAAGGGGATAATGTTGGTGACGGTGAAGGAGGTTTCTCTGTTGAAGATAACGATGAAGATGAAGAATTTTTAGATAGTGAATACGAAGATGAAGATGATTCCGGTGATGTGAATGATGATGATTATGGTGATGTAAATGAAGATGATTCTGGTGATGTGAATGATGATGATTCCGGTGATGTGAATGGAAATTCTGATTCAGAGGGATACACTGATGAAAATGTTGATGACGATTTGGAAGGAAATTATGATGATGTCAATGAATCAACATCTTCACATCCAAGTCTAGAAGAGTCGTTTGTAATAGATGGTGTTGAAGATATTGTAAAGATGAACATGTTTAACTTGCAAATTGAAGATGTTGCAAAGTTGCAATTCGGGAGCCTGGAAGTTGCCTACAATTTTTATTGTTGGTTTGCAAAGATGAACGGTTTTGCTGTCAGAAAAGGTCAAGTTATTAAAAATAAGAACGGTGATGTTCTTCAACAAACATTTGTTTGCAATCTAGAAGGGTTCAGGAAAGACAGAGGTCTAACAGCAGAGGAGCGTAAGCGTGGTCCAGTACATGAAACCCGTTGCGGTTGTGGAGAAAAATTTCGAGTCCATATCGATGTAATCACGCATCGTTGGTACATTACAGTTTTCATCTTTGATCACAATCATGAAATGTTGAGTGACAAACATTGTGGGTTGTTGGCTGCTCATAGGAAGCTCAGTGAGTCAGACAAGATTCAAATTAAGTACTTTGGTAATGCTGGAATCAAAGTTGGTCAAATGATTGGTGCGTTTGCAAATGCTGCCGGTGGGTATGATAAGGTAGGATTTCTGAAGAAGGATTTACATAATCAAATTCTTAGACAGAGAAAATCGATGTCATCTGATGCTAAAGGTGCTGTTAGATATCTTAAAGATTTGCGTTCTAAAGATCCATTGATGTTTGTTTCACATACGGTTGATGCGGAAGGTAGGCTGCAACACTTATTTTGGAGCGACGGTGAAAGTCAAAAGAATTTTGAAGTTTTTGGCGATGTACTTGCTTTTGATGCCACATATAAGAAGAATAAATATAGGTGTCCTTTTGTCTTGTTTTCTAGTGTTAATCACCACAACCAGACAATTATATTTGGTACCGCTGTTGTTTCAAATGAGGTTGAAGGGACGTACGTGTGGTTGTTGGAGCAGTTATTGGTTGCAATGAAAGGTAAGACACCGGTAGCTGTAATAACAGATGGGGATCTAGCTATGAGGAATGCGATCAAGGAAGTTTTTCCAAAGAGTTATCATAGGTTATGTGCATGGCATCTACTTCGAAATGCAATGTCCAACATTGGTAATCTTGATTTCATCCCTTTTTTCAAAAGATGTATGCTTGGTGATTATGAAGTCTGGAAATTTGAAAAACTTTGGAATGAAATGGTTGAAAGGTTTGGTTTTGAAGAAAACAGATGGATTAATGAATTGTATGAGAAAAGGAAAATGTGGGCAACAAGTCATATTCGAGGAAATTTCTTTGCAGGGATAAGAACAACATCAAGGTGTGAGGCATTTCACAGTCATATGAAGCAGTTCATACACTCAAAAATGAACATGACCGATTTTGTTAAGCACTTTCATAGGTGTGTTGCATATTTTCGCTTTAAAGAGGTTCAAGCCGATCTTAATTCCCAATACGGGACTGTAGTGTTGAAGACCAATCTTAGGTCGCTTGAGAGGTCTGCCTCAAAACAGTTTACCAAAGAAATTTTTGAGATGGTACGATCTGTCATCCAAAAGGTATCCCTAGTATCTTTGTTAGACACCCAAAATATGTCTTGTTCTACAATTTATTCATTGACAAAGTATCGAGATGAAGGTTATGTCTGGTCTGTTTCGCATAGTCCATTGGACAATCAATTTGCATGTTCTTGTCTTAGAATGGAATCTATAGGAATTCCTTACGAGCATATCATAGCGGTGCTGGTATATCTTGATTTTGTTGATTTTCCCGAGTCTCTAGTGTTGAATCGTTGGTCACAATTTGCAAAGGAGTCAATAAGGGGAAATTACGAAGATGGTTCACATTACTGGGATTCGCATTTGGTTGCTAGGCATGCGAATTTGGTTTATCTTAGCAAGGAAGTTTCTGACCTAGCTTACAAGGATGTTGATGATTATAAAAATTATTTGGAATATCTAACAAATGAAATTAACAGGCTTAAATCAAAGTACTCCAATGAAGGTGGACCAGAGAATTTACATGTTCCAGGGGAGTTGGATAATATACTTAATCCTTCATGTTCAAGAAGCAAAGGGTGTGGGCATAGCACGGGTGGTACAATGGAAAGACGTCGACGGACACAGACTTGTAGTATATGCGGTGCAGCAGGACACAACAGACGATCTTGTCCTACACTTGATGCAACTGGAGAAGTAGTTTCTGACATGTCCTTACAAACAGAGTCTTTACGATTAAGCAATGACAAGTAG
Protein sequence

>MS.gene71270.t1

MNHRGFVSATSLWLYFFPARPLAAAVLVYGICTDSNGNDAGDSVGHEVMQAYYQAMDELTHENGGGNIVGEGDNVGDGEGEGYTDENVDDDLEGNYDDVNESTSSHPSLEESFVIDGVEDIVKMNMFNLQIEDVAKLQFGSLEVAYNFYCWFAKMNGFAVRKGQVIKNKNGDVLQQTFVCNLEGFRKDRGLTAEERKRGPVHETRCGCGEKFRVHIDVITHRWYITVFIFDHNHEMLSDKHCGLLAAHRKLSESDKIQIKYFGNAGIKVGQMIGAFANAADPLMFVSHTVDAEGRLQHLFWSDGESQKNFEVFGDVLAFDATYKKNKYRCPFVLFSSVNHHNQTIIFGTAVVSNEVEGTYVWLLEQLLVAMKGKTPVAVITDGDLAMRNAIKEVFPKSYHRLCAWHLLRNAMSNIGNLDFIPFFKRCMLGDYEVWKFEKLWNEMVERFGFEENRWINELYEKRKMWATSHIRGNFFAGIRTTSRCEAFHSHMKQFIHSKMNMTDFVKHFHRCVAYFRFKEVQADLNSQYGTVVLKTNLRSLERMESIGIPYEHIIAVLVYLDFVDFPESLVLNRWSQFAKESIRGNYEDGSHYWDSHLVARHANLVYLSKEVSDLAYKDVDDYKNYLEYLTNEINRLKSKYSNEGGPENLHVPGELDNILNPSCSRSKGCGHSTGGTMERRRRTQTCSICGAAGHNRRSCPTLDATGEVVSDMSLQTESLRLSNDK