Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71354.t1 | AES92029.1 | 53.5 | 198 | 13 | 1 | 5 | 123 | 28 | 225 | 7.80E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71354.t1 | G7JQ00 | 53.5 | 198 | 13 | 1 | 5 | 123 | 28 | 225 | 5.6e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene71354.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71354.t1 | MTR_4g123470 | 86.905 | 84 | 11 | 0 | 40 | 123 | 142 | 225 | 1.00e-51 | 163 |
| MS.gene71354.t1 | MTR_4g123470 | 86.842 | 38 | 5 | 0 | 5 | 42 | 28 | 65 | 1.05e-17 | 75.9 |
| MS.gene71354.t1 | MTR_7g113060 | 84.211 | 38 | 6 | 0 | 84 | 121 | 2 | 39 | 3.92e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAACTACCTGATGCTTT+TGG | 0.332739 | 7.2:-87356437 | MS.gene71354:CDS |
| AATCGTGATGCTTAACTCTA+AGG | 0.346259 | 7.2:+87356408 | None:intergenic |
| ACCGGAGAGATGGCACTTGC+TGG | 0.380086 | 7.2:-87356831 | MS.gene71354:CDS |
| TCCGAACATCACATCTGGTA+TGG | 0.430547 | 7.2:+87356322 | None:intergenic |
| AGGAATTTGCCAAAAGCATC+AGG | 0.443467 | 7.2:+87356428 | None:intergenic |
| CCGAACATCACATCTGGTAT+GGG | 0.460591 | 7.2:+87356323 | None:intergenic |
| TTGTGTTGCCAATGGTATTG+AGG | 0.460704 | 7.2:-87356282 | MS.gene71354:CDS |
| CCAGATGTGATGTTCGGAAA+AGG | 0.491103 | 7.2:-87356317 | MS.gene71354:CDS |
| TGTGTTGCCAATGGTATTGA+GGG | 0.494690 | 7.2:-87356281 | MS.gene71354:CDS |
| CCCATACCAGATGTGATGTT+CGG | 0.499659 | 7.2:-87356323 | MS.gene71354:CDS |
| TAACGTCGTGTTCAACAAAT+CGG | 0.502741 | 7.2:-87356789 | MS.gene71354:intron |
| TTGTCTAACTTCTAGGATGT+TGG | 0.504778 | 7.2:-87356896 | MS.gene71354:intron |
| TCAATATTTCTACGAGCTAC+CGG | 0.507354 | 7.2:-87356849 | MS.gene71354:CDS |
| AAATATTGAGCAACGAAGAA+TGG | 0.513098 | 7.2:+87356863 | None:intergenic |
| TCCAGCAAGTGCCATCTCTC+CGG | 0.524122 | 7.2:+87356830 | None:intergenic |
| ACAGGAATGATATTGTCATA+AGG | 0.528831 | 7.2:+87356368 | None:intergenic |
| TCTGTATATTTGAGTCTCAC+AGG | 0.555272 | 7.2:+87356350 | None:intergenic |
| GTCTCCGCCCTCAATACCAT+TGG | 0.566866 | 7.2:+87356274 | None:intergenic |
| TTTCAAGTGCAAGTGCGTCA+TGG | 0.567746 | 7.2:-87356243 | MS.gene71354:CDS |
| CATAAGGTCCGCACAACATG+AGG | 0.581838 | 7.2:+87356384 | None:intergenic |
| ATTTAAATATATATGATGAA+CGG | 0.587288 | 7.2:-87357029 | None:intergenic |
| TGGCACTTGCTGGATGGTGA+AGG | 0.588023 | 7.2:-87356821 | MS.gene71354:CDS |
| TCTACGAGCTACCGGAGAGA+TGG | 0.600729 | 7.2:-87356841 | MS.gene71354:CDS |
| CACGATTACCTCATGTTGTG+CGG | 0.605525 | 7.2:-87356392 | MS.gene71354:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.616871 | 7.2:-87356313 | MS.gene71354:CDS |
| GGCACTTGCTGGATGGTGAA+GGG | 0.623602 | 7.2:-87356820 | MS.gene71354:CDS |
| GAGAGATGGCACTTGCTGGA+TGG | 0.637149 | 7.2:-87356827 | MS.gene71354:CDS |
| CGAACATCACATCTGGTATG+GGG | 0.646248 | 7.2:+87356324 | None:intergenic |
| GTTGCCAATGGTATTGAGGG+CGG | 0.712333 | 7.2:-87356278 | MS.gene71354:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCTGATTTTTTTTTTTTA+AGG | - | chr7.2:87356772-87356791 | MS.gene71354:intron | 10.0% |
| !! | TTAGACAATGTATTAAAAAA+AGG | + | chr7.2:87356320-87356339 | None:intergenic | 15.0% |
| !!! | TTTTTTGTTGTTGTTTAAAT+CGG | - | chr7.2:87356279-87356298 | MS.gene71354:CDS | 15.0% |
| !! | AAAAAACAATAAACAACCTT+CGG | + | chr7.2:87356695-87356714 | None:intergenic | 20.0% |
| !!! | ATGAAAATTAAATCCTCTTT+TGG | - | chr7.2:87356244-87356263 | MS.gene71354:CDS | 20.0% |
| ! | TAATACATTGTCTAACTTCT+AGG | - | chr7.2:87356325-87356344 | MS.gene71354:CDS | 25.0% |
| !!! | TTTTAAAGCTGTTCAATTAG+AGG | - | chr7.2:87356712-87356731 | MS.gene71354:intron | 25.0% |
| AAATATTGAGCAACGAAGAA+TGG | + | chr7.2:87356368-87356387 | None:intergenic | 30.0% | |
| ACAGGAATGATATTGTCATA+AGG | + | chr7.2:87356863-87356882 | None:intergenic | 30.0% | |
| ACATTAACTTCAACCAAAAG+AGG | + | chr7.2:87356260-87356279 | None:intergenic | 30.0% | |
| AGATTATTCTACGAGTTTAG+TGG | - | chr7.2:87356500-87356519 | MS.gene71354:intron | 30.0% | |
| ATTATTGCTTTCCAATACCT+TGG | - | chr7.2:87356530-87356549 | MS.gene71354:intron | 30.0% | |
| TCATGCTCATTAAAAGTCTA+CGG | + | chr7.2:87356228-87356247 | None:intergenic | 30.0% | |
| AATCGTGATGCTTAACTCTA+AGG | + | chr7.2:87356823-87356842 | None:intergenic | 35.0% | |
| GAAATTCCTATCACTGGATA+TGG | - | chr7.2:87356656-87356675 | MS.gene71354:intron | 35.0% | |
| TAACGTCGTGTTCAACAAAT+CGG | - | chr7.2:87356439-87356458 | MS.gene71354:intron | 35.0% | |
| TCCAGTGATAGGAATTTCAA+AGG | + | chr7.2:87356654-87356673 | None:intergenic | 35.0% | |
| TCTGTATATTTGAGTCTCAC+AGG | + | chr7.2:87356881-87356900 | None:intergenic | 35.0% | |
| TGTTCAACAAATCGGTTAGT+CGG | - | chr7.2:87356447-87356466 | MS.gene71354:intron | 35.0% | |
| ! | TCAATATTTCTACGAGCTAC+CGG | - | chr7.2:87356379-87356398 | MS.gene71354:CDS | 35.0% |
| ! | TTTGAAATCTACTTCCACCA+AGG | + | chr7.2:87356550-87356569 | None:intergenic | 35.0% |
| !! | ACCTTTGAAATTCCTATCAC+TGG | - | chr7.2:87356650-87356669 | MS.gene71354:intron | 35.0% |
| !! | TTGTCTAACTTCTAGGATGT+TGG | - | chr7.2:87356332-87356351 | MS.gene71354:CDS | 35.0% |
| ATCGATCCATATCCAGTGAT+AGG | + | chr7.2:87356665-87356684 | None:intergenic | 40.0% | |
| ATCTACTTCCACCAAGGTAT+TGG | + | chr7.2:87356544-87356563 | None:intergenic | 40.0% | |
| ATGTGATGTTCGGAAAAGGT+TGG | - | chr7.2:87356915-87356934 | MS.gene71354:intron | 40.0% | |
| ATTGCTTTCCAATACCTTGG+TGG | - | chr7.2:87356533-87356552 | MS.gene71354:intron | 40.0% | |
| TTGTGTTGCCAATGGTATTG+AGG | - | chr7.2:87356946-87356965 | MS.gene71354:intron | 40.0% | |
| ! | AAGGAACTACCTGATGCTTT+TGG | - | chr7.2:87356791-87356810 | MS.gene71354:CDS | 40.0% |
| ! | ACGAGTTTTTGTGTTGCCAA+TGG | - | chr7.2:87356938-87356957 | MS.gene71354:intron | 40.0% |
| ! | TGTGTTGCCAATGGTATTGA+GGG | - | chr7.2:87356947-87356966 | MS.gene71354:intron | 40.0% |
| !! | AGGAATTTGCCAAAAGCATC+AGG | + | chr7.2:87356803-87356822 | None:intergenic | 40.0% |
| ACCGTATCATTCCATGAGTG+CGG | - | chr7.2:87356610-87356629 | MS.gene71354:intron | 45.0% | |
| CACGATTACCTCATGTTGTG+CGG | - | chr7.2:87356836-87356855 | MS.gene71354:CDS | 45.0% | |
| CACTCATGGAATGATACGGT+GGG | + | chr7.2:87356610-87356629 | None:intergenic | 45.0% | |
| CCAGATGTGATGTTCGGAAA+AGG | - | chr7.2:87356911-87356930 | MS.gene71354:intron | 45.0% | |
| CCCATACCAGATGTGATGTT+CGG | - | chr7.2:87356905-87356924 | MS.gene71354:intron | 45.0% | |
| CCGAACATCACATCTGGTAT+GGG | + | chr7.2:87356908-87356927 | None:intergenic | 45.0% | |
| CGAACATCACATCTGGTATG+GGG | + | chr7.2:87356907-87356926 | None:intergenic | 45.0% | |
| CTCCAACCATCTGTCAACAA+CGG | + | chr7.2:87356473-87356492 | None:intergenic | 45.0% | |
| TCCGAACATCACATCTGGTA+TGG | + | chr7.2:87356909-87356928 | None:intergenic | 45.0% | |
| TGGATCGATGAAATCACCGA+AGG | - | chr7.2:87356676-87356695 | MS.gene71354:intron | 45.0% | |
| ! | CCTTTTCCGAACATCACATC+TGG | + | chr7.2:87356914-87356933 | None:intergenic | 45.0% |
| ! | TTTCAAGTGCAAGTGCGTCA+TGG | - | chr7.2:87356985-87357004 | MS.gene71354:intron | 45.0% |
| CATAAGGTCCGCACAACATG+AGG | + | chr7.2:87356847-87356866 | None:intergenic | 50.0% | |
| GCACTCATGGAATGATACGG+TGG | + | chr7.2:87356611-87356630 | None:intergenic | 50.0% | |
| GCCGCACTCATGGAATGATA+CGG | + | chr7.2:87356614-87356633 | None:intergenic | 50.0% | |
| TGATACGGTGGGTAATCAGC+TGG | + | chr7.2:87356599-87356618 | None:intergenic | 50.0% | |
| ! | GTTGCCAATGGTATTGAGGG+CGG | - | chr7.2:87356950-87356969 | MS.gene71354:intron | 50.0% |
| ACATGATGCTGCCGCACTCA+TGG | + | chr7.2:87356624-87356643 | None:intergenic | 55.0% | |
| AGTCGGCCGTTGTTGACAGA+TGG | - | chr7.2:87356464-87356483 | MS.gene71354:intron | 55.0% | |
| GGCCGTTGTTGACAGATGGT+TGG | - | chr7.2:87356468-87356487 | MS.gene71354:intron | 55.0% | |
| GTCTCCGCCCTCAATACCAT+TGG | + | chr7.2:87356957-87356976 | None:intergenic | 55.0% | |
| TCCAGCAAGTGCCATCTCTC+CGG | + | chr7.2:87356401-87356420 | None:intergenic | 55.0% | |
| TCTACGAGCTACCGGAGAGA+TGG | - | chr7.2:87356387-87356406 | MS.gene71354:CDS | 55.0% | |
| ! | GAGAGATGGCACTTGCTGGA+TGG | - | chr7.2:87356401-87356420 | MS.gene71354:CDS | 55.0% |
| ! | GGCACTTGCTGGATGGTGAA+GGG | - | chr7.2:87356408-87356427 | MS.gene71354:CDS | 55.0% |
| ! | TGGCACTTGCTGGATGGTGA+AGG | - | chr7.2:87356407-87356426 | MS.gene71354:CDS | 55.0% |
| ACCGGAGAGATGGCACTTGC+TGG | - | chr7.2:87356397-87356416 | MS.gene71354:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 87356211 | 87357039 | 87356211 | ID=MS.gene71354 |
| chr7.2 | mRNA | 87356211 | 87357039 | 87356211 | ID=MS.gene71354.t1;Parent=MS.gene71354 |
| chr7.2 | exon | 87357028 | 87357039 | 87357028 | ID=MS.gene71354.t1.exon1;Parent=MS.gene71354.t1 |
| chr7.2 | CDS | 87357028 | 87357039 | 87357028 | ID=cds.MS.gene71354.t1;Parent=MS.gene71354.t1 |
| chr7.2 | exon | 87356790 | 87356903 | 87356790 | ID=MS.gene71354.t1.exon2;Parent=MS.gene71354.t1 |
| chr7.2 | CDS | 87356790 | 87356903 | 87356790 | ID=cds.MS.gene71354.t1;Parent=MS.gene71354.t1 |
| chr7.2 | exon | 87356211 | 87356456 | 87356211 | ID=MS.gene71354.t1.exon3;Parent=MS.gene71354.t1 |
| chr7.2 | CDS | 87356211 | 87356456 | 87356211 | ID=cds.MS.gene71354.t1;Parent=MS.gene71354.t1 |
| Gene Sequence |
| Protein sequence |
