AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71389

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71389.t1 MTR_5g017980 95.370 324 15 0 2 325 3 326 0.0 570
MS.gene71389.t1 MTR_5g017980 95.062 324 15 1 2 325 3 325 0.0 563
MS.gene71389.t1 MTR_5g017980 95.062 324 14 2 2 325 3 324 0.0 559
MS.gene71389.t1 MTR_4g086835 40.887 406 148 13 5 324 8 407 2.50e-66 214
MS.gene71389.t1 MTR_4g086835 40.640 406 148 14 5 324 8 406 6.19e-64 208
MS.gene71389.t1 MTR_4g113140 35.831 307 138 8 9 263 6 305 2.93e-40 146
MS.gene71389.t1 MTR_1g093080 33.434 332 140 13 34 293 2 324 4.45e-39 142
MS.gene71389.t1 MTR_5g054300 34.981 263 103 8 39 253 17 259 5.16e-36 134
MS.gene71389.t1 MTR_1g093080 34.875 281 114 11 34 254 2 273 3.96e-32 121
MS.gene71389.t1 MTR_1g093080 30.165 242 100 11 34 215 2 234 6.56e-16 76.6
MS.gene71389.t1 MTR_7g088070 50.588 85 32 2 162 246 30 104 1.38e-14 73.2
MS.gene71389.t1 MTR_7g088070 50.588 85 32 2 162 246 30 104 1.46e-14 73.2
MS.gene71389.t1 MTR_3g106220 39.200 125 64 5 191 306 197 318 2.18e-14 74.3
MS.gene71389.t1 MTR_2g027860 55.714 70 30 1 192 261 49 117 3.27e-14 72.8
MS.gene71389.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 4.90e-14 73.2
MS.gene71389.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 4.95e-14 73.2
MS.gene71389.t1 MTR_4g121020 57.377 61 25 1 191 251 110 169 5.38e-14 73.2
MS.gene71389.t1 MTR_5g041350 60.000 55 22 0 192 246 15 69 5.88e-14 72.4
MS.gene71389.t1 MTR_1g090670 54.688 64 29 0 183 246 35 98 1.95e-13 70.1
MS.gene71389.t1 MTR_1g032570 48.649 74 37 1 189 262 128 200 2.79e-13 70.9
MS.gene71389.t1 MTR_2g034960 50.000 72 35 1 189 260 208 278 3.13e-13 70.9
MS.gene71389.t1 MTR_1g032570 48.649 74 37 1 189 262 197 269 3.16e-13 70.9
MS.gene71389.t1 MTR_4g081710 42.697 89 50 1 192 279 17 105 3.43e-13 69.7
MS.gene71389.t1 MTR_1g013170 50.769 65 31 1 187 251 186 249 3.65e-13 70.5
MS.gene71389.t1 MTR_5g014040 33.088 136 63 4 117 251 261 369 4.14e-13 70.5
MS.gene71389.t1 MTR_1g053830 50.877 57 28 0 190 246 16 72 4.55e-13 65.1
MS.gene71389.t1 MTR_7g089010 49.206 63 31 1 188 250 87 148 5.11e-13 68.9
MS.gene71389.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 6.53e-13 68.6
MS.gene71389.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 7.42e-13 68.2
MS.gene71389.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 8.23e-13 68.9
MS.gene71389.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 8.25e-13 68.6
MS.gene71389.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 1.73e-12 67.4
MS.gene71389.t1 MTR_7g098250 52.239 67 31 1 189 255 259 324 1.77e-12 68.2
MS.gene71389.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 1.98e-12 67.8
MS.gene71389.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 2.29e-12 67.4
MS.gene71389.t1 MTR_3g064840 46.988 83 40 1 163 245 249 327 2.76e-12 67.8
MS.gene71389.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.79e-12 68.2
MS.gene71389.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.87e-12 67.8
MS.gene71389.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 2.96e-12 67.0
MS.gene71389.t1 MTR_5g027440 38.000 100 49 2 164 261 8 96 3.12e-12 66.2
MS.gene71389.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 3.23e-12 67.0
MS.gene71389.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 4.79e-12 67.0
MS.gene71389.t1 MTR_4g064730 45.312 64 34 1 188 251 127 189 6.05e-12 65.9
MS.gene71389.t1 MTR_1g053835 54.386 57 26 0 190 246 256 312 8.93e-12 66.2
MS.gene71389.t1 MTR_2g086450 38.679 106 50 2 141 246 9 99 9.39e-12 65.9
MS.gene71389.t1 MTR_6g071625 37.815 119 57 3 158 270 175 282 1.27e-11 65.5
MS.gene71389.t1 MTR_1g112370 59.184 49 20 0 197 245 27 75 1.39e-11 65.1
MS.gene71389.t1 MTR_6g032990 52.727 55 26 0 192 246 42 96 1.84e-11 65.1
MS.gene71389.t1 MTR_5g029470 31.061 132 78 2 153 284 184 302 2.20e-11 64.7
MS.gene71389.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 2.38e-11 63.9
MS.gene71389.t1 MTR_4g131580 50.000 60 29 1 191 250 199 257 3.02e-11 64.7
MS.gene71389.t1 MTR_7g117705 45.714 70 37 1 191 260 190 258 3.91e-11 64.3
MS.gene71389.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 4.91e-11 63.9
MS.gene71389.t1 MTR_8g066310 50.820 61 30 0 185 245 166 226 5.03e-11 63.5
MS.gene71389.t1 MTR_4g098870 43.939 66 36 1 186 251 343 407 5.08e-11 63.9
MS.gene71389.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 5.19e-11 63.5
MS.gene71389.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 5.27e-11 63.9
MS.gene71389.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 6.29e-11 63.9
MS.gene71389.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 6.46e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71389.t1 AT4G37180 40.055 362 156 11 6 329 12 350 2.51e-60 197
MS.gene71389.t1 AT4G37180 38.859 368 159 11 6 329 12 357 1.83e-57 190
MS.gene71389.t1 AT4G37180 43.985 266 103 8 6 242 12 260 1.76e-51 172
MS.gene71389.t1 AT2G03500 38.811 286 131 9 9 250 6 291 3.65e-44 157
MS.gene71389.t1 AT1G68670 35.816 282 117 8 31 256 6 279 1.77e-40 145
MS.gene71389.t1 AT1G25550 36.727 275 113 9 31 256 10 272 5.08e-39 141
MS.gene71389.t1 AT1G49560 56.154 130 37 4 180 290 171 299 9.94e-35 129
MS.gene71389.t1 AT1G25550 35.249 261 109 8 31 242 10 259 7.22e-33 123
MS.gene71389.t1 AT1G13300 85.000 60 9 0 191 250 180 239 4.40e-29 114
MS.gene71389.t1 AT1G49560 70.833 72 12 1 180 242 171 242 5.88e-26 104
MS.gene71389.t1 AT3G25790 81.818 55 10 0 196 250 200 254 1.97e-25 105
MS.gene71389.t1 AT3G25790 80.851 47 9 0 196 242 200 246 2.65e-20 89.0
MS.gene71389.t1 AT2G01060 54.930 71 31 1 185 255 8 77 2.90e-14 72.4
MS.gene71389.t1 AT2G20400 44.828 87 47 1 192 278 231 316 3.61e-14 73.2
MS.gene71389.t1 AT2G20400 46.512 86 43 2 192 277 231 313 1.39e-13 71.6
MS.gene71389.t1 AT2G40970 43.478 92 44 3 159 250 79 162 1.51e-13 69.7
MS.gene71389.t1 AT2G06020 50.000 64 32 0 183 246 77 140 1.70e-13 70.5
MS.gene71389.t1 AT5G05090 51.667 60 28 1 191 250 80 138 1.87e-13 69.7
MS.gene71389.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.88e-13 70.1
MS.gene71389.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.88e-13 70.1
MS.gene71389.t1 AT3G10760 51.667 60 28 1 191 250 104 162 2.85e-13 70.1
MS.gene71389.t1 AT5G59570 46.875 64 33 1 188 251 137 199 3.44e-13 69.7
MS.gene71389.t1 AT5G59570 46.875 64 33 1 188 251 137 199 3.44e-13 69.7
MS.gene71389.t1 AT3G24120 60.000 55 22 0 192 246 41 95 3.89e-13 69.3
MS.gene71389.t1 AT3G24120 60.000 55 22 0 192 246 41 95 4.19e-13 69.3
MS.gene71389.t1 AT3G16857 54.098 61 27 1 191 251 166 225 8.09e-13 69.7
MS.gene71389.t1 AT3G12730 58.182 55 23 0 192 246 23 77 1.01e-12 67.4
MS.gene71389.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.02e-12 69.3
MS.gene71389.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.03e-12 69.3
MS.gene71389.t1 AT5G58080 49.206 63 31 1 189 251 191 252 1.31e-12 68.9
MS.gene71389.t1 AT3G04030 56.364 55 24 0 192 246 45 99 2.72e-12 67.4
MS.gene71389.t1 AT5G06800 54.545 55 25 0 192 246 192 246 2.92e-12 66.6
MS.gene71389.t1 AT1G67710 50.746 67 33 0 185 251 121 187 2.99e-12 67.8
MS.gene71389.t1 AT1G67710 50.746 67 33 0 185 251 112 178 3.05e-12 67.8
MS.gene71389.t1 AT3G04030 56.364 55 24 0 192 246 45 99 3.16e-12 67.4
MS.gene71389.t1 AT1G67710 50.746 67 33 0 185 251 186 252 3.70e-12 67.4
MS.gene71389.t1 AT1G79430 60.000 55 22 0 192 246 34 88 3.85e-12 67.0
MS.gene71389.t1 AT3G46640 47.541 61 31 1 191 251 143 202 4.57e-12 66.6
MS.gene71389.t1 AT3G46640 47.541 61 31 1 191 251 143 202 4.57e-12 66.6
MS.gene71389.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.29e-12 67.0
MS.gene71389.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.29e-12 67.0
MS.gene71389.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.29e-12 67.0
MS.gene71389.t1 AT3G04450 50.820 61 30 0 185 245 231 291 5.77e-12 66.2
MS.gene71389.t1 AT4G17695 41.237 97 47 2 177 272 148 235 5.83e-12 66.2
MS.gene71389.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.14e-12 66.6
MS.gene71389.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.14e-12 66.6
MS.gene71389.t1 AT3G46640 47.541 61 31 1 191 251 143 202 6.61e-12 65.9
MS.gene71389.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.69e-12 66.2
MS.gene71389.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.91e-12 66.2
MS.gene71389.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.91e-12 66.2
MS.gene71389.t1 AT5G45580 56.364 55 24 0 192 246 7 61 6.97e-12 65.5
MS.gene71389.t1 AT5G45580 56.364 55 24 0 192 246 23 77 7.48e-12 65.5
MS.gene71389.t1 AT5G06800 54.545 55 25 0 192 246 192 246 7.94e-12 66.2
MS.gene71389.t1 AT5G06800 54.545 55 25 0 192 246 192 246 8.24e-12 65.9
MS.gene71389.t1 AT1G32240 38.776 98 51 1 182 279 203 291 9.55e-12 65.9
MS.gene71389.t1 AT3G04450 50.820 61 30 0 185 245 231 291 1.14e-11 65.9
MS.gene71389.t1 AT1G32240 38.776 98 51 1 182 279 203 291 1.15e-11 65.5
MS.gene71389.t1 AT2G25180 46.377 69 36 1 191 259 194 261 1.32e-11 65.9
MS.gene71389.t1 AT2G01760 50.000 62 30 1 190 251 133 193 1.32e-11 65.1
MS.gene71389.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.36e-11 65.5
MS.gene71389.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.36e-11 65.5
MS.gene71389.t1 AT2G01760 50.000 62 30 1 190 251 226 286 1.51e-11 65.5
MS.gene71389.t1 AT2G01760 50.000 62 30 1 190 251 198 258 1.66e-11 65.1
MS.gene71389.t1 AT1G69580 50.909 55 27 0 192 246 34 88 1.92e-11 64.7
MS.gene71389.t1 AT5G29000 52.632 57 27 0 189 245 228 284 2.01e-11 64.3
MS.gene71389.t1 AT1G69580 50.909 55 27 0 192 246 34 88 2.04e-11 64.7
MS.gene71389.t1 AT4G31920 40.789 76 44 1 189 264 180 254 2.15e-11 65.1
MS.gene71389.t1 AT5G16560 51.562 64 31 0 182 245 209 272 2.35e-11 64.7
MS.gene71389.t1 AT4G28610 55.556 54 24 0 192 245 225 278 3.20e-11 64.3
MS.gene71389.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.65e-11 63.5
MS.gene71389.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.65e-11 63.5
MS.gene71389.t1 AT5G29000 52.632 57 27 0 189 245 228 284 4.78e-11 63.9
MS.gene71389.t1 AT5G42630 53.333 60 28 0 186 245 99 158 5.77e-11 62.0
MS.gene71389.t1 AT4G04605 53.571 56 26 0 190 245 12 67 8.68e-11 61.6
MS.gene71389.t1 AT2G02060 47.059 68 35 1 178 245 16 82 9.28e-11 62.0
MS.gene71389.t1 AT2G02060 47.059 68 35 1 178 245 16 82 9.28e-11 62.0

Find 76 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGGTGGAGGGGCATTTA+TGG 0.148750 5.1:-10253619 MS.gene71389:CDS
CAGCCACTCCTAAGCAAATT+AGG 0.199099 5.1:-10252829 MS.gene71389:CDS
CCAGCACCTCCTTTGTTTCC+TGG 0.269810 5.1:+10253428 None:intergenic
AAAATTAATTACCATCATTA+AGG 0.282228 5.1:+10253997 None:intergenic
ATTCCCAGTTTCGTCAATTC+AGG 0.284606 5.1:-10252598 MS.gene71389:CDS
AGCAATCAGATTGCAGGAAT+TGG 0.302511 5.1:-10252404 MS.gene71389:CDS
AACCTTTGCAGCAGAGTTCC+AGG 0.309685 5.1:-10253446 MS.gene71389:CDS
AAAAGTTGGGTTGATGAAAA+TGG 0.310662 5.1:-10253868 MS.gene71389:CDS
CTCCTTGGTGGATCTGTTCA+AGG 0.312173 5.1:-10252468 MS.gene71389:CDS
GCCTCCTGAATTGACGAAAC+TGG 0.313674 5.1:+10252594 None:intergenic
AAGAGTGGTGGTGGTAGTTC+TGG 0.356168 5.1:-10253516 MS.gene71389:CDS
AAATTTCTGAGACATGTCTT+TGG 0.371563 5.1:+10254109 None:intergenic
AATGGTGGAGGGGCATTTAT+GGG 0.391222 5.1:-10253618 MS.gene71389:CDS
AGCCACTCCTAAGCAAATTA+GGG 0.392450 5.1:-10252828 MS.gene71389:CDS
CTCAGAGTCTGTTTCTCCAC+AGG 0.393005 5.1:-10252508 MS.gene71389:CDS
ACCATCATTAAGGAGGAGTA+TGG 0.396799 5.1:+10254007 None:intergenic
ACTGTGATAACAAGAAGAAT+TGG 0.397769 5.1:-10253830 MS.gene71389:CDS
CATCCATACTGTTTCTTCCA+TGG 0.409764 5.1:+10252436 None:intergenic
TTGTGATTATGCAGAAGTAT+AGG 0.413901 5.1:-10252635 MS.gene71389:intron
CCTCTTACTCCTCTTCTCCT+TGG 0.417068 5.1:-10252483 MS.gene71389:CDS
AAGATGAGCAATCAGATTGC+AGG 0.425444 5.1:-10252410 MS.gene71389:CDS
TAATAAGTTAGCCTTGTATA+TGG 0.434530 5.1:-10252571 MS.gene71389:CDS
AGAACTACCACCACCACTCT+TGG 0.439523 5.1:+10253518 None:intergenic
AAGCCATGGAAGAAACAGTA+TGG 0.440126 5.1:-10252439 MS.gene71389:CDS
CTTACTCCTCTTCTCCTTGG+TGG 0.445651 5.1:-10252480 MS.gene71389:CDS
ACAATGAAGGTGATGATGAT+AGG 0.445952 5.1:-10253659 MS.gene71389:CDS
TAAGGAGGAGTATGGAAAGT+GGG 0.450917 5.1:+10254015 None:intergenic
TGATGACTTGGTTCATAGCT+TGG 0.452822 5.1:-10254078 MS.gene71389:CDS
TATTGTCACTCACTTGCAAA+TGG 0.459590 5.1:+10252762 None:intergenic
TCACAGAAGGGTAATGGTGG+AGG 0.470703 5.1:-10253630 MS.gene71389:CDS
CCAAGGAGAAGAGGAGTAAG+AGG 0.473664 5.1:+10252483 None:intergenic
TCCATACTCCTCCTTAATGA+TGG 0.476669 5.1:-10254008 MS.gene71389:intron
TTCCCTAATTTGCTTAGGAG+TGG 0.487933 5.1:+10252826 None:intergenic
ACAAGCTAAGAGCTTCAGTC+TGG 0.491107 5.1:-10253565 MS.gene71389:CDS
TGCAAAGGTTGAGGCGGCTG+AGG 0.496741 5.1:+10253459 None:intergenic
TTAAGGAGGAGTATGGAAAG+TGG 0.497298 5.1:+10254014 None:intergenic
CAGGAATTGGAAAAGTGATC+AGG 0.500799 5.1:-10252391 MS.gene71389:CDS
ATTTAATGAAAATCTGTTGA+AGG 0.503957 5.1:-10253595 MS.gene71389:CDS
TTCCTGGAACTCTGCTGCAA+AGG 0.504800 5.1:+10253444 None:intergenic
GTTGAAGTGCATCAACAAAT+CGG 0.508453 5.1:+10253388 None:intergenic
GAACTCTGCTGCAAAGGTTG+AGG 0.519079 5.1:+10253450 None:intergenic
CCTCCTGAATTGACGAAACT+GGG 0.520520 5.1:+10252595 None:intergenic
CCAGGAAACAAAGGAGGTGC+TGG 0.526510 5.1:-10253428 MS.gene71389:CDS
ATGGACTGATGAAACAAAAC+CGG 0.528150 5.1:-10253790 MS.gene71389:intron
GTTCAAGGTCTTTCAAGCCA+TGG 0.531430 5.1:-10252453 MS.gene71389:CDS
AGGGAAAAGATGCAAGTGGA+TGG 0.538779 5.1:-10252809 MS.gene71389:CDS
TCAGAGTCTGTTTCTCCACA+GGG 0.539092 5.1:-10252507 MS.gene71389:CDS
GGATGAGTTCAGCTCAGCTA+TGG 0.541177 5.1:-10253809 MS.gene71389:CDS
GTTGTTGAGGAAGTGAGAAA+CGG 0.546665 5.1:+10254142 None:intergenic
TAATGAAAATCTGTTGAAGG+AGG 0.547757 5.1:-10253592 MS.gene71389:CDS
TGTGAAGAAGATACATCAAA+GGG 0.552624 5.1:-10252537 MS.gene71389:CDS
ATTAATTACCATCATTAAGG+AGG 0.553744 5.1:+10254000 None:intergenic
GGAAATTCCAAGAGTGGTGG+TGG 0.558490 5.1:-10253525 MS.gene71389:CDS
CCCAGTTTCGTCAATTCAGG+AGG 0.558843 5.1:-10252595 MS.gene71389:CDS
ATTGTGTCACAGAAGGGTAA+TGG 0.560958 5.1:-10253636 MS.gene71389:CDS
CACAGAAGGGTAATGGTGGA+GGG 0.561226 5.1:-10253629 MS.gene71389:CDS
CAACAGCTTGGTGGAGCACA+TGG 0.565013 5.1:-10253369 MS.gene71389:intron
GTTGATGCACTTCAACAGCT+TGG 0.565906 5.1:-10253381 MS.gene71389:CDS
ACAGATCCACCAAGGAGAAG+AGG 0.569987 5.1:+10252474 None:intergenic
TGATAGGATTGTGTCACAGA+AGG 0.576368 5.1:-10253643 MS.gene71389:CDS
ATGTGAAGAAGATACATCAA+AGG 0.582691 5.1:-10252538 MS.gene71389:CDS
TGGTTTGAACAACGTTGTTG+AGG 0.582932 5.1:+10254129 None:intergenic
GATGCACTTCAACAGCTTGG+TGG 0.583631 5.1:-10253378 MS.gene71389:CDS
CTCTGCTGCAAAGGTTGAGG+CGG 0.585862 5.1:+10253453 None:intergenic
AGCAGAGTTCCAGGAAACAA+AGG 0.588997 5.1:-10253437 MS.gene71389:CDS
GATAGGATTGTGTCACAGAA+GGG 0.591671 5.1:-10253642 MS.gene71389:CDS
AATTAGGGAAAAGATGCAAG+TGG 0.615575 5.1:-10252813 MS.gene71389:CDS
TTGATCTTGAGCCATATACA+AGG 0.616538 5.1:+10252560 None:intergenic
ACAGAAGGGTAATGGTGGAG+GGG 0.617241 5.1:-10253628 MS.gene71389:CDS
CTGGTGTCTGAAGTGAGTCA+TGG 0.635526 5.1:-10253546 MS.gene71389:CDS
AGTCATGGAAATTCCAAGAG+TGG 0.640027 5.1:-10253531 MS.gene71389:CDS
CATGGAAATTCCAAGAGTGG+TGG 0.649869 5.1:-10253528 MS.gene71389:CDS
AGAGGAGTAAGAGGACCCTG+TGG 0.650364 5.1:+10252492 None:intergenic
GTGTCACAGAAGGGTAATGG+TGG 0.658081 5.1:-10253633 MS.gene71389:CDS
AGAGTTCCAGGAAACAAAGG+AGG 0.672803 5.1:-10253434 MS.gene71389:CDS
GACCTTGAACAGATCCACCA+AGG 0.687479 5.1:+10252466 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCACAATTAAAAAAAA+AGG + chr5.1:10252831-10252850 None:intergenic 10.0%
!! CTAAAAAATATTAAAATTAC+CGG + chr5.1:10252794-10252813 None:intergenic 10.0%
!!! AAAAAAAAATGTTCTTTTTT+TGG - chr5.1:10252876-10252895 MS.gene71389:intron 10.0%
!! AAAATTAACAACAAAGAAAA+AGG + chr5.1:10252634-10252653 None:intergenic 15.0%
!! AAAATTAATTACCATCATTA+AGG + chr5.1:10252568-10252587 None:intergenic 15.0%
!! AATGATGAAAAAGAAAAAGT+TGG - chr5.1:10252680-10252699 MS.gene71389:intron 20.0%
!! ATGATGAAAAAGAAAAAGTT+GGG - chr5.1:10252681-10252700 MS.gene71389:intron 20.0%
!! ATTTAATGAAAATCTGTTGA+AGG - chr5.1:10252967-10252986 MS.gene71389:intron 20.0%
!!! AGTTCTAAGTTATAACAAAT+AGG + chr5.1:10253608-10253627 None:intergenic 20.0%
!!! TTGTAATGATTACTGTTTTT+TGG - chr5.1:10252602-10252621 MS.gene71389:CDS 20.0%
! AATCAATCTTAGTCGTTATA+TGG - chr5.1:10253428-10253447 MS.gene71389:CDS 25.0%
! AGATTACATGACATGTTATT+AGG - chr5.1:10253568-10253587 MS.gene71389:CDS 25.0%
! ATTAATTACCATCATTAAGG+AGG + chr5.1:10252565-10252584 None:intergenic 25.0%
! GATTACATGACATGTTATTA+GGG - chr5.1:10253569-10253588 MS.gene71389:CDS 25.0%
!! ATGTTATTAGGGATTAGTAT+AGG - chr5.1:10253580-10253599 MS.gene71389:CDS 25.0%
!! CAATTTGGAATTGAAATTGT+GGG - chr5.1:10253338-10253357 MS.gene71389:intron 25.0%
!!! TAATAAGTTAGCCTTGTATA+TGG - chr5.1:10253991-10254010 MS.gene71389:intron 25.0%
!!! TCTCTATTTTGTTTTGGATT+TGG - chr5.1:10253298-10253317 MS.gene71389:intron 25.0%
!!! TCTTAGTCTCTATTTTGTTT+TGG - chr5.1:10253292-10253311 MS.gene71389:intron 25.0%
!!! TTTTTTTGGTAGAACAATGA+AGG - chr5.1:10252890-10252909 MS.gene71389:intron 25.0%
ACTAATAATCGTGTTAACCA+TGG + chr5.1:10253849-10253868 None:intergenic 30.0%
ACTGTGATAACAAGAAGAAT+TGG - chr5.1:10252732-10252751 MS.gene71389:intron 30.0%
ATGTGAAGAAGATACATCAA+AGG - chr5.1:10254024-10254043 MS.gene71389:CDS 30.0%
CCACAATTTCAATTCCAAAT+TGG + chr5.1:10253340-10253359 None:intergenic 30.0%
GCTCTGATTTATTATGGTAT+AGG - chr5.1:10253264-10253283 MS.gene71389:intron 30.0%
TAATGAAAATCTGTTGAAGG+AGG - chr5.1:10252970-10252989 MS.gene71389:intron 30.0%
TACAAAAAAGAGAACCAGTT+AGG + chr5.1:10253712-10253731 None:intergenic 30.0%
TAGAACTGTTCGATTAAGAT+TGG - chr5.1:10253621-10253640 MS.gene71389:CDS 30.0%
TGAAATTGTGGGTTTGTATT+GGG - chr5.1:10253349-10253368 MS.gene71389:intron 30.0%
TGTGAAGAAGATACATCAAA+GGG - chr5.1:10254025-10254044 MS.gene71389:CDS 30.0%
TTGAAATTGTGGGTTTGTAT+TGG - chr5.1:10253348-10253367 MS.gene71389:intron 30.0%
! AAATTTTGCAATCAGCAAGA+TGG + chr5.1:10253653-10253672 None:intergenic 30.0%
! AGTGTTGCTCTGATTTATTA+TGG - chr5.1:10253258-10253277 MS.gene71389:intron 30.0%
! ATAGAGTTGATTCTCCAATT+TGG - chr5.1:10253323-10253342 MS.gene71389:intron 30.0%
! CCAATTTGGAATTGAAATTG+TGG - chr5.1:10253337-10253356 MS.gene71389:intron 30.0%
! TTGTGATTATGCAGAAGTAT+AGG - chr5.1:10253927-10253946 MS.gene71389:intron 30.0%
!! AAAAGTTGGGTTGATGAAAA+TGG - chr5.1:10252694-10252713 MS.gene71389:intron 30.0%
!! AAATTTCTGAGACATGTCTT+TGG + chr5.1:10252456-10252475 None:intergenic 30.0%
!! ATTTTCAATGCTTGATGACT+TGG - chr5.1:10252472-10252491 MS.gene71389:CDS 30.0%
!!! AAAATTTTTTGACCGTACAC+AGG - chr5.1:10253666-10253685 MS.gene71389:intron 30.0%
!!! ATCTTTTCCCTAATTTGCTT+AGG + chr5.1:10253744-10253763 None:intergenic 30.0%
AACTGTTCGATTAAGATTGG+AGG - chr5.1:10253624-10253643 MS.gene71389:CDS 35.0%
AATCTAGATTACCTGCAGTT+AGG - chr5.1:10253382-10253401 MS.gene71389:CDS 35.0%
AATTAGGGAAAAGATGCAAG+TGG - chr5.1:10253749-10253768 MS.gene71389:intron 35.0%
ATAATCGTGTTAACCATGGA+TGG + chr5.1:10253845-10253864 None:intergenic 35.0%
GTTGAAGTGCATCAACAAAT+CGG + chr5.1:10253177-10253196 None:intergenic 35.0%
TATTGTCACTCACTTGCAAA+TGG + chr5.1:10253803-10253822 None:intergenic 35.0%
TTGATCTTGAGCCATATACA+AGG + chr5.1:10254005-10254024 None:intergenic 35.0%
! ACAATGAAGGTGATGATGAT+AGG - chr5.1:10252903-10252922 MS.gene71389:intron 35.0%
! ATGGACTGATGAAACAAAAC+CGG - chr5.1:10252772-10252791 MS.gene71389:intron 35.0%
AAGATGAGCAATCAGATTGC+AGG - chr5.1:10254152-10254171 MS.gene71389:CDS 40.0%
AAGCCATGGAAGAAACAGTA+TGG - chr5.1:10254123-10254142 MS.gene71389:CDS 40.0%
AATTGTGGGTTTGTATTGGG+TGG - chr5.1:10253352-10253371 MS.gene71389:intron 40.0%
ACCATCATTAAGGAGGAGTA+TGG + chr5.1:10252558-10252577 None:intergenic 40.0%
ACCTGCAGTTAGGAAATATG+TGG - chr5.1:10253392-10253411 MS.gene71389:CDS 40.0%
AGCAATCAGATTGCAGGAAT+TGG - chr5.1:10254158-10254177 MS.gene71389:CDS 40.0%
AGCCACTCCTAAGCAAATTA+GGG - chr5.1:10253734-10253753 MS.gene71389:intron 40.0%
AGTCATGGAAATTCCAAGAG+TGG - chr5.1:10253031-10253050 MS.gene71389:intron 40.0%
ATTCCCAGTTTCGTCAATTC+AGG - chr5.1:10253964-10253983 MS.gene71389:intron 40.0%
ATTGTGGGTTTGTATTGGGT+GGG - chr5.1:10253353-10253372 MS.gene71389:intron 40.0%
ATTGTGTCACAGAAGGGTAA+TGG - chr5.1:10252926-10252945 MS.gene71389:intron 40.0%
CAGGAATTGGAAAAGTGATC+AGG - chr5.1:10254171-10254190 MS.gene71389:CDS 40.0%
GAATCCCACTCTTGTGTATA+GGG - chr5.1:10253541-10253560 MS.gene71389:CDS 40.0%
GATAGGATTGTGTCACAGAA+GGG - chr5.1:10252920-10252939 MS.gene71389:intron 40.0%
GTTGTAAGATGCAGATCAGA+TGG + chr5.1:10253504-10253523 None:intergenic 40.0%
GTTGTTGAGGAAGTGAGAAA+CGG + chr5.1:10252423-10252442 None:intergenic 40.0%
TCCACATATTTCCTAACTGC+AGG + chr5.1:10253396-10253415 None:intergenic 40.0%
TCCATACTCCTCCTTAATGA+TGG - chr5.1:10252554-10252573 MS.gene71389:CDS 40.0%
TGATAGGATTGTGTCACAGA+AGG - chr5.1:10252919-10252938 MS.gene71389:intron 40.0%
TGATCCCTATACACAAGAGT+GGG + chr5.1:10253548-10253567 None:intergenic 40.0%
TGGTTTGAACAACGTTGTTG+AGG + chr5.1:10252436-10252455 None:intergenic 40.0%
TTGATCCCTATACACAAGAG+TGG + chr5.1:10253549-10253568 None:intergenic 40.0%
! AGGAAGTGAGAAACGGTTTT+TGG + chr5.1:10252416-10252435 None:intergenic 40.0%
! CATCCATACTGTTTCTTCCA+TGG + chr5.1:10254129-10254148 None:intergenic 40.0%
! TAAGGAGGAGTATGGAAAGT+GGG + chr5.1:10252550-10252569 None:intergenic 40.0%
! TTAAGGAGGAGTATGGAAAG+TGG + chr5.1:10252551-10252570 None:intergenic 40.0%
!! TGATGACTTGGTTCATAGCT+TGG - chr5.1:10252484-10252503 MS.gene71389:CDS 40.0%
!! TTCCCTAATTTGCTTAGGAG+TGG + chr5.1:10253739-10253758 None:intergenic 40.0%
!!! AGAAACGGTTTTTGGAACAG+AGG + chr5.1:10252408-10252427 None:intergenic 40.0%
AAGATGCAGATCAGATGGCT+TGG + chr5.1:10253499-10253518 None:intergenic 45.0%
ACAACTGAGACAACCACTAC+AGG - chr5.1:10253519-10253538 MS.gene71389:CDS 45.0%
ACAAGCTAAGAGCTTCAGTC+TGG - chr5.1:10252997-10253016 MS.gene71389:intron 45.0%
AGAGTTCCAGGAAACAAAGG+AGG - chr5.1:10253128-10253147 MS.gene71389:intron 45.0%
AGCAGAGTTCCAGGAAACAA+AGG - chr5.1:10253125-10253144 MS.gene71389:intron 45.0%
AGGGAAAAGATGCAAGTGGA+TGG - chr5.1:10253753-10253772 MS.gene71389:intron 45.0%
CAGCCACTCCTAAGCAAATT+AGG - chr5.1:10253733-10253752 MS.gene71389:intron 45.0%
CATGGAAATTCCAAGAGTGG+TGG - chr5.1:10253034-10253053 MS.gene71389:intron 45.0%
CCTCCTGAATTGACGAAACT+GGG + chr5.1:10253970-10253989 None:intergenic 45.0%
CGTGTTAACCATGGATGGTT+TGG + chr5.1:10253840-10253859 None:intergenic 45.0%
CTCAAACTCCAAACCATCCA+TGG - chr5.1:10253829-10253848 MS.gene71389:CDS 45.0%
GGAATCCCACTCTTGTGTAT+AGG - chr5.1:10253540-10253559 MS.gene71389:CDS 45.0%
GTTCAAGGTCTTTCAAGCCA+TGG - chr5.1:10254109-10254128 MS.gene71389:CDS 45.0%
GTTGATGCACTTCAACAGCT+TGG - chr5.1:10253181-10253200 MS.gene71389:intron 45.0%
TTGTGGGTTTGTATTGGGTG+GGG - chr5.1:10253354-10253373 MS.gene71389:intron 45.0%
! TCAGAGTCTGTTTCTCCACA+GGG - chr5.1:10254055-10254074 MS.gene71389:CDS 45.0%
!! AATGGTGGAGGGGCATTTAT+GGG - chr5.1:10252944-10252963 MS.gene71389:intron 45.0%
!! TAATGGTGGAGGGGCATTTA+TGG - chr5.1:10252943-10252962 MS.gene71389:intron 45.0%
AACCTTTGCAGCAGAGTTCC+AGG - chr5.1:10253116-10253135 MS.gene71389:intron 50.0%
ACAGATCCACCAAGGAGAAG+AGG + chr5.1:10254091-10254110 None:intergenic 50.0%
AGAACTACCACCACCACTCT+TGG + chr5.1:10253047-10253066 None:intergenic 50.0%
AGGGTGTGTATGAGCCTAAC+TGG - chr5.1:10253695-10253714 MS.gene71389:intron 50.0%
CAAGAGTGGGATTCCTGTAG+TGG + chr5.1:10253535-10253554 None:intergenic 50.0%
CACCCTACATAGCCTGTGTA+CGG + chr5.1:10253681-10253700 None:intergenic 50.0%
CCAAGGAGAAGAGGAGTAAG+AGG + chr5.1:10254082-10254101 None:intergenic 50.0%
CCCAGTTTCGTCAATTCAGG+AGG - chr5.1:10253967-10253986 MS.gene71389:intron 50.0%
CCTCTTACTCCTCTTCTCCT+TGG - chr5.1:10254079-10254098 MS.gene71389:CDS 50.0%
CTTACTCCTCTTCTCCTTGG+TGG - chr5.1:10254082-10254101 MS.gene71389:CDS 50.0%
GAACTCTGCTGCAAAGGTTG+AGG + chr5.1:10253115-10253134 None:intergenic 50.0%
GACCGTACACAGGCTATGTA+GGG - chr5.1:10253676-10253695 MS.gene71389:intron 50.0%
GACCTTGAACAGATCCACCA+AGG + chr5.1:10254099-10254118 None:intergenic 50.0%
GATGCACTTCAACAGCTTGG+TGG - chr5.1:10253184-10253203 MS.gene71389:intron 50.0%
GCCTCCTGAATTGACGAAAC+TGG + chr5.1:10253971-10253990 None:intergenic 50.0%
GGAAATTCCAAGAGTGGTGG+TGG - chr5.1:10253037-10253056 MS.gene71389:intron 50.0%
GGATGAGTTCAGCTCAGCTA+TGG - chr5.1:10252753-10252772 MS.gene71389:intron 50.0%
TGACCGTACACAGGCTATGT+AGG - chr5.1:10253675-10253694 MS.gene71389:intron 50.0%
TGTGGGTTTGTATTGGGTGG+GGG - chr5.1:10253355-10253374 MS.gene71389:intron 50.0%
TTCCTGGAACTCTGCTGCAA+AGG + chr5.1:10253121-10253140 None:intergenic 50.0%
! ACAGAAGGGTAATGGTGGAG+GGG - chr5.1:10252934-10252953 MS.gene71389:intron 50.0%
! CACAGAAGGGTAATGGTGGA+GGG - chr5.1:10252933-10252952 MS.gene71389:intron 50.0%
! CTCAGAGTCTGTTTCTCCAC+AGG - chr5.1:10254054-10254073 MS.gene71389:CDS 50.0%
! CTCCTTGGTGGATCTGTTCA+AGG - chr5.1:10254094-10254113 MS.gene71389:CDS 50.0%
! CTGGTGTCTGAAGTGAGTCA+TGG - chr5.1:10253016-10253035 MS.gene71389:intron 50.0%
! GTGTCACAGAAGGGTAATGG+TGG - chr5.1:10252929-10252948 MS.gene71389:intron 50.0%
! TCACAGAAGGGTAATGGTGG+AGG - chr5.1:10252932-10252951 MS.gene71389:intron 50.0%
!! AAGAGTGGTGGTGGTAGTTC+TGG - chr5.1:10253046-10253065 MS.gene71389:intron 50.0%
AGAGGAGTAAGAGGACCCTG+TGG + chr5.1:10254073-10254092 None:intergenic 55.0%
CTCTGCTGCAAAGGTTGAGG+CGG + chr5.1:10253112-10253131 None:intergenic 55.0%
GTGGGTTTGTATTGGGTGGG+GGG - chr5.1:10253356-10253375 MS.gene71389:intron 55.0%
! CAACAGCTTGGTGGAGCACA+TGG - chr5.1:10253193-10253212 MS.gene71389:intron 55.0%
! CCAGCACCTCCTTTGTTTCC+TGG + chr5.1:10253137-10253156 None:intergenic 55.0%
! CCAGGAAACAAAGGAGGTGC+TGG - chr5.1:10253134-10253153 MS.gene71389:intron 55.0%
TGCAAAGGTTGAGGCGGCTG+AGG + chr5.1:10253106-10253125 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 10252368 10254216 10252368 ID=MS.gene71389
chr5.1 mRNA 10252368 10254216 10252368 ID=MS.gene71389.t1;Parent=MS.gene71389
chr5.1 exon 10254009 10254216 10254009 ID=MS.gene71389.t1.exon1;Parent=MS.gene71389.t1
chr5.1 CDS 10254009 10254216 10254009 ID=cds.MS.gene71389.t1;Parent=MS.gene71389.t1
chr5.1 exon 10253791 10253918 10253791 ID=MS.gene71389.t1.exon2;Parent=MS.gene71389.t1
chr5.1 CDS 10253791 10253918 10253791 ID=cds.MS.gene71389.t1;Parent=MS.gene71389.t1
chr5.1 exon 10253370 10253682 10253370 ID=MS.gene71389.t1.exon3;Parent=MS.gene71389.t1
chr5.1 CDS 10253370 10253682 10253370 ID=cds.MS.gene71389.t1;Parent=MS.gene71389.t1
chr5.1 exon 10252775 10252851 10252775 ID=MS.gene71389.t1.exon4;Parent=MS.gene71389.t1
chr5.1 CDS 10252775 10252851 10252775 ID=cds.MS.gene71389.t1;Parent=MS.gene71389.t1
chr5.1 exon 10252368 10252643 10252368 ID=MS.gene71389.t1.exon5;Parent=MS.gene71389.t1
chr5.1 CDS 10252368 10252643 10252368 ID=cds.MS.gene71389.t1;Parent=MS.gene71389.t1
Gene Sequence

>MS.gene71389

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCACTTCCTCAACAACGTTGTTCAAACCAAAGACATGTCTCAGAAATTTTCAATGCTTGATGACTTGGTTCATAGCTTGGAAGAAGAAATGAAGAAAGTTCTTGCTTTCAAACGTGAACTCCCACTTTCCATACTCCTCCTTAATGATGGTAATTAATTTTGTTAATTAATTTTTTTGTAATGATTACTGTTTTTTGGATTTAACCTTTTTCTTTGTTGTTAATTTTTAATTTTTGTAGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGATGATTTGAAAGAAAACTGTGATAACAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTGATGAAACAAAACCGGTAATTTTAATATTTTTTAGAAATTAGTAAATTACCTTTTTTTTTAATTGTGATTTTTGTTTATTGAAGTGAGATTTGAGATAAAAAAAAATGTTCTTTTTTTGGTAGAACAATGAAGGTGATGATGATAGGATTGTGTCACAGAAGGGTAATGGTGGAGGGGCATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTCAGTCTGGTGTCTGAAGTGAGTCATGGAAATTCCAAGAGTGGTGGTGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCTCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCTGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGGTAAATTGTTTTTGTTCAGATTGATTGTTAATTAGTTGTGTTAAGTGTTGCTCTGATTTATTATGGTATAGGAATTTTCTTAGTCTCTATTTTGTTTTGGATTTGGTAATAGAGTTGATTCTCCAATTTGGAATTGAAATTGTGGGTTTGTATTGGGTGGGGGGAGCAATCTAGATTACCTGCAGTTAGGAAATATGTGGATTTAGTGTATTTAATCAATCTTAGTCGTTATATGGATATCATACGTTTTTATAGTTGTGTAAAATAGTTCAGCTTGAAATCCAAGCCATCTGATCTGCATCTTACAACTGAGACAACCACTACAGGAATCCCACTCTTGTGTATAGGGATCAAGATTACATGACATGTTATTAGGGATTAGTATAGGCACCTATTTGTTATAACTTAGAACTGTTCGATTAAGATTGGAGGTTTCCATCTTGCTGATTGCAAAATTTTTTGACCGTACACAGGCTATGTAGGGTGTGTATGAGCCTAACTGGTTCTCTTTTTTGTAGCAGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAGTGAGTGACAATATTTCATCTCAAACTCCAAACCATCCATGGTTAACACGATTATTAGTTTCTTCGTATTAATTTGATTCTTTTGTTATGTCATGTCAAGCAATTGATGTAGCACATTTGTGATTATGCAGAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA
Protein sequence

>MS.gene71389.t1

MEQQQLNLDLTLASVPKTVSHFLNNVVQTKDMSQKFSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMDDLKENCDNKKNWMSSAQLWTDETKPNNEGDDDRIVSQKGNGGGAFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGGGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHAATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE