Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71445.t1 | XP_003590803.1 | 86.4 | 191 | 22 | 2 | 1 | 187 | 1 | 191 | 9.40E-65 | 256.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71445.t1 | Q9SR34 | 64.4 | 45 | 15 | 1 | 4 | 48 | 20 | 63 | 3.1e-08 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71445.t1 | G7I4R8 | 86.4 | 191 | 22 | 2 | 1 | 187 | 1 | 191 | 6.8e-65 | 256.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene71445.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71445.t1 | MTR_1g075430 | 90.576 | 191 | 14 | 2 | 1 | 187 | 1 | 191 | 6.03e-125 | 350 |
| MS.gene71445.t1 | MTR_1g044225 | 40.314 | 191 | 72 | 10 | 1 | 183 | 5 | 161 | 1.38e-23 | 92.0 |
| MS.gene71445.t1 | MTR_7g063220 | 75.610 | 41 | 10 | 0 | 12 | 52 | 18 | 58 | 7.32e-17 | 73.6 |
| MS.gene71445.t1 | MTR_4g064873 | 58.730 | 63 | 19 | 2 | 11 | 69 | 10 | 69 | 3.82e-16 | 72.8 |
| MS.gene71445.t1 | MTR_1g070250 | 36.207 | 174 | 85 | 7 | 14 | 180 | 34 | 188 | 3.82e-13 | 65.1 |
| MS.gene71445.t1 | MTR_4g021270 | 69.444 | 36 | 11 | 0 | 18 | 53 | 59 | 94 | 3.80e-12 | 63.9 |
| MS.gene71445.t1 | MTR_5g014400 | 57.143 | 49 | 20 | 1 | 18 | 65 | 80 | 128 | 1.40e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71445.t1 | AT5G43540 | 68.293 | 41 | 13 | 0 | 20 | 60 | 35 | 75 | 8.54e-15 | 68.2 |
| MS.gene71445.t1 | AT3G09290 | 83.871 | 31 | 5 | 0 | 18 | 48 | 33 | 63 | 1.84e-13 | 65.5 |
| MS.gene71445.t1 | AT3G53820 | 83.871 | 31 | 5 | 0 | 18 | 48 | 19 | 49 | 1.48e-12 | 62.4 |
| MS.gene71445.t1 | AT3G23140 | 55.102 | 49 | 21 | 1 | 1 | 48 | 1 | 49 | 8.27e-12 | 61.2 |
| MS.gene71445.t1 | AT5G06070 | 41.667 | 84 | 32 | 2 | 4 | 70 | 38 | 121 | 2.09e-11 | 60.8 |
| MS.gene71445.t1 | AT2G37740 | 33.654 | 104 | 51 | 2 | 7 | 110 | 26 | 111 | 3.48e-11 | 61.2 |
| MS.gene71445.t1 | AT3G23130 | 68.571 | 35 | 11 | 0 | 18 | 52 | 45 | 79 | 5.27e-11 | 59.7 |
Find 37 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATCATTGGTGTAGAATT+TGG | 0.194932 | 1.3:-53925646 | None:intergenic |
| TAATTTCCAACATGGTAATT+TGG | 0.204844 | 1.3:-53925783 | None:intergenic |
| TCATTTCCTCTCCTAATTCT+TGG | 0.246256 | 1.3:-53925939 | None:intergenic |
| AATTCTTCTTCATCACAAAT+TGG | 0.251144 | 1.3:+53925621 | MS.gene71445:CDS |
| TCAGAACAAAATTCCTTTGT+TGG | 0.304478 | 1.3:+53925690 | MS.gene71445:CDS |
| ATTTGTGATGAAGAAGAATT+TGG | 0.304515 | 1.3:-53925618 | None:intergenic |
| ATACACATCATCCAAGAATT+AGG | 0.310498 | 1.3:+53925928 | MS.gene71445:CDS |
| TTCACAAATGCACAAGCTTT+AGG | 0.350501 | 1.3:+53925546 | MS.gene71445:CDS |
| ATGGCAATGATCAAGGAAAA+AGG | 0.379483 | 1.3:+53925490 | MS.gene71445:CDS |
| CCTGACTTGAGTCTTCAAAT+AGG | 0.388896 | 1.3:+53925885 | MS.gene71445:CDS |
| GTTGGATATTCATATGATCT+TGG | 0.400343 | 1.3:-53925756 | None:intergenic |
| CTACACCAATGATGAAACTA+TGG | 0.411572 | 1.3:+53925653 | MS.gene71445:CDS |
| CCTTAGCTCTATCTTTCCTA+TGG | 0.421858 | 1.3:-53925584 | None:intergenic |
| CTCTTGGTTCAAATGCCAAT+TGG | 0.423506 | 1.3:-53925853 | None:intergenic |
| AAAGACCATAGTTTCATCAT+TGG | 0.428141 | 1.3:-53925658 | None:intergenic |
| AGTCAGGATGTTGAAGCTCT+TGG | 0.457420 | 1.3:-53925869 | None:intergenic |
| AATCATGCATATTTGAAGGT+TGG | 0.470730 | 1.3:-53925719 | None:intergenic |
| ATGAATTATTCAAGTGAAGA+AGG | 0.471381 | 1.3:+53925462 | MS.gene71445:CDS |
| GGAGGAAAATGAAGTGGACT+TGG | 0.473209 | 1.3:+53925965 | MS.gene71445:CDS |
| AATTAGGAGAGGAAATGATC+AGG | 0.477244 | 1.3:+53925944 | MS.gene71445:CDS |
| ACATGGTAATTTGGTGCTGT+TGG | 0.496509 | 1.3:-53925774 | None:intergenic |
| GATATCAAGGACCATCCAAT+TGG | 0.511436 | 1.3:+53925838 | MS.gene71445:CDS |
| TCAAGTGAAGAAGGTGATGA+TGG | 0.515818 | 1.3:+53925471 | MS.gene71445:CDS |
| CCTATTTGAAGACTCAAGTC+AGG | 0.518186 | 1.3:-53925885 | None:intergenic |
| GGTGATGATGGCAATGATCA+AGG | 0.525173 | 1.3:+53925483 | MS.gene71445:CDS |
| TATCAACCTTCAAATGTGCA+TGG | 0.548381 | 1.3:+53925804 | MS.gene71445:CDS |
| TGGTTCAAATGCCAATTGGA+TGG | 0.560601 | 1.3:-53925849 | None:intergenic |
| TGATCAGGAGGAAAATGAAG+TGG | 0.569482 | 1.3:+53925959 | MS.gene71445:CDS |
| GTGGACTTGGAGCTAAGACT+TGG | 0.578657 | 1.3:+53925978 | MS.gene71445:CDS |
| TAGGAGAGGAAATGATCAGG+AGG | 0.592497 | 1.3:+53925947 | MS.gene71445:CDS |
| ACAGCACCAAATTACCATGT+TGG | 0.599662 | 1.3:+53925777 | MS.gene71445:CDS |
| AGGTTGGTAATTTCCAACAA+AGG | 0.601009 | 1.3:-53925703 | None:intergenic |
| GTGGTCACATGAATATCCAT+AGG | 0.605103 | 1.3:+53925568 | MS.gene71445:CDS |
| AAGGTTGATAATTTCCAACA+TGG | 0.615290 | 1.3:-53925791 | None:intergenic |
| CCATAGGAAAGATAGAGCTA+AGG | 0.615552 | 1.3:+53925584 | MS.gene71445:CDS |
| ACAAATGCACAAGCTTTAGG+TGG | 0.646984 | 1.3:+53925549 | MS.gene71445:CDS |
| CATCATCCAAGAATTAGGAG+AGG | 0.685725 | 1.3:+53925933 | MS.gene71445:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTCTTCTTCATCACAAAT+TGG | + | chr1.3:53925621-53925640 | MS.gene71445:CDS | 25.0% |
| ! | ATGAATTATTCAAGTGAAGA+AGG | + | chr1.3:53925462-53925481 | MS.gene71445:CDS | 25.0% |
| ! | ATTTGTGATGAAGAAGAATT+TGG | - | chr1.3:53925621-53925640 | None:intergenic | 25.0% |
| ! | GAAAAATCATGCATATTTGA+AGG | - | chr1.3:53925726-53925745 | None:intergenic | 25.0% |
| ! | TAATTTCCAACATGGTAATT+TGG | - | chr1.3:53925786-53925805 | None:intergenic | 25.0% |
| AAAGACCATAGTTTCATCAT+TGG | - | chr1.3:53925661-53925680 | None:intergenic | 30.0% | |
| AATCATGCATATTTGAAGGT+TGG | - | chr1.3:53925722-53925741 | None:intergenic | 30.0% | |
| ATACACATCATCCAAGAATT+AGG | + | chr1.3:53925928-53925947 | MS.gene71445:CDS | 30.0% | |
| GTTGGATATTCATATGATCT+TGG | - | chr1.3:53925759-53925778 | None:intergenic | 30.0% | |
| TCAGAACAAAATTCCTTTGT+TGG | + | chr1.3:53925690-53925709 | MS.gene71445:CDS | 30.0% | |
| TTCATCATTGGTGTAGAATT+TGG | - | chr1.3:53925649-53925668 | None:intergenic | 30.0% | |
| ! | AATGTGCATGGTTTTTCTTA+TGG | + | chr1.3:53925816-53925835 | MS.gene71445:CDS | 30.0% |
| ! | GGTTTTTCTTATGGATATCA+AGG | + | chr1.3:53925825-53925844 | MS.gene71445:CDS | 30.0% |
| !! | AAGGTTGATAATTTCCAACA+TGG | - | chr1.3:53925794-53925813 | None:intergenic | 30.0% |
| !!! | GAATTTTGTTCTGAAGCAAA+AGG | - | chr1.3:53925684-53925703 | None:intergenic | 30.0% |
| AATTAGGAGAGGAAATGATC+AGG | + | chr1.3:53925944-53925963 | MS.gene71445:CDS | 35.0% | |
| ATGGCAATGATCAAGGAAAA+AGG | + | chr1.3:53925490-53925509 | MS.gene71445:CDS | 35.0% | |
| CTACACCAATGATGAAACTA+TGG | + | chr1.3:53925653-53925672 | MS.gene71445:CDS | 35.0% | |
| GAAAAACCATGCACATTTGA+AGG | - | chr1.3:53925813-53925832 | None:intergenic | 35.0% | |
| TATCAACCTTCAAATGTGCA+TGG | + | chr1.3:53925804-53925823 | MS.gene71445:CDS | 35.0% | |
| TCATTTCCTCTCCTAATTCT+TGG | - | chr1.3:53925942-53925961 | None:intergenic | 35.0% | |
| TTCACAAATGCACAAGCTTT+AGG | + | chr1.3:53925546-53925565 | MS.gene71445:CDS | 35.0% | |
| ! | GAATGCACATTTTGCAAAAG+AGG | + | chr1.3:53925522-53925541 | MS.gene71445:CDS | 35.0% |
| !! | AGGTTGGTAATTTCCAACAA+AGG | - | chr1.3:53925706-53925725 | None:intergenic | 35.0% |
| ACAGCACCAAATTACCATGT+TGG | + | chr1.3:53925777-53925796 | MS.gene71445:CDS | 40.0% | |
| CATCATCCAAGAATTAGGAG+AGG | + | chr1.3:53925933-53925952 | MS.gene71445:CDS | 40.0% | |
| CCATAGGAAAGATAGAGCTA+AGG | + | chr1.3:53925584-53925603 | MS.gene71445:CDS | 40.0% | |
| CCTATTTGAAGACTCAAGTC+AGG | - | chr1.3:53925888-53925907 | None:intergenic | 40.0% | |
| CTCTTGGTTCAAATGCCAAT+TGG | - | chr1.3:53925856-53925875 | None:intergenic | 40.0% | |
| GATATCAAGGACCATCCAAT+TGG | + | chr1.3:53925838-53925857 | MS.gene71445:CDS | 40.0% | |
| GTGGTCACATGAATATCCAT+AGG | + | chr1.3:53925568-53925587 | MS.gene71445:CDS | 40.0% | |
| TCAAGTGAAGAAGGTGATGA+TGG | + | chr1.3:53925471-53925490 | MS.gene71445:CDS | 40.0% | |
| TGATCAGGAGGAAAATGAAG+TGG | + | chr1.3:53925959-53925978 | MS.gene71445:CDS | 40.0% | |
| TGGTTCAAATGCCAATTGGA+TGG | - | chr1.3:53925852-53925871 | None:intergenic | 40.0% | |
| ! | CCTGACTTGAGTCTTCAAAT+AGG | + | chr1.3:53925885-53925904 | MS.gene71445:CDS | 40.0% |
| !! | ACAAATGCACAAGCTTTAGG+TGG | + | chr1.3:53925549-53925568 | MS.gene71445:CDS | 40.0% |
| !! | ACATGGTAATTTGGTGCTGT+TGG | - | chr1.3:53925777-53925796 | None:intergenic | 40.0% |
| !! | CCTTAGCTCTATCTTTCCTA+TGG | - | chr1.3:53925587-53925606 | None:intergenic | 40.0% |
| AGTCAGGATGTTGAAGCTCT+TGG | - | chr1.3:53925872-53925891 | None:intergenic | 45.0% | |
| TAGGAGAGGAAATGATCAGG+AGG | + | chr1.3:53925947-53925966 | MS.gene71445:CDS | 45.0% | |
| ! | GGAGGAAAATGAAGTGGACT+TGG | + | chr1.3:53925965-53925984 | MS.gene71445:CDS | 45.0% |
| !! | GGTGATGATGGCAATGATCA+AGG | + | chr1.3:53925483-53925502 | MS.gene71445:CDS | 45.0% |
| GTGGACTTGGAGCTAAGACT+TGG | + | chr1.3:53925978-53925997 | MS.gene71445:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 53925462 | 53926025 | 53925462 | ID=MS.gene71445 |
| chr1.3 | mRNA | 53925462 | 53926025 | 53925462 | ID=MS.gene71445.t1;Parent=MS.gene71445 |
| chr1.3 | exon | 53925462 | 53926025 | 53925462 | ID=MS.gene71445.t1.exon1;Parent=MS.gene71445.t1 |
| chr1.3 | CDS | 53925462 | 53926025 | 53925462 | ID=cds.MS.gene71445.t1;Parent=MS.gene71445.t1 |
| Gene Sequence |
| Protein sequence |
