AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71585.t1 MTR_1g105960 93.578 109 7 0 1 109 1 109 1.48e-70 206
MS.gene71585.t1 MTR_1g105955 53.846 117 35 1 2 99 17 133 2.66e-34 117
MS.gene71585.t1 MTR_1g105955 65.217 69 24 0 2 70 96 164 1.69e-24 92.0
MS.gene71585.t1 MTR_8g062700 74.667 75 19 0 2 76 43 117 1.98e-32 119
MS.gene71585.t1 MTR_8g062700 74.667 75 19 0 2 76 43 117 2.04e-32 119
MS.gene71585.t1 MTR_5g036670 66.234 77 26 0 2 78 44 120 6.64e-30 112
MS.gene71585.t1 MTR_6g008970 68.000 75 24 0 2 76 64 138 1.04e-28 108
MS.gene71585.t1 MTR_1g104940 58.974 78 32 0 2 79 15 92 5.01e-26 94.0
MS.gene71585.t1 MTR_6g008995 62.667 75 26 1 2 76 38 110 1.16e-24 96.7
MS.gene71585.t1 MTR_1g104960 52.703 74 35 0 2 75 100 173 5.60e-22 85.1
MS.gene71585.t1 MTR_1g104960 38.017 121 49 1 2 96 14 134 5.50e-18 75.1
MS.gene71585.t1 MTR_3g006065 65.152 66 22 1 2 66 91 156 3.22e-21 83.6
MS.gene71585.t1 MTR_3g006065 41.463 123 43 3 2 98 9 128 3.22e-18 75.9
MS.gene71585.t1 MTR_3g006150 65.152 66 22 1 2 66 91 156 3.56e-21 82.8
MS.gene71585.t1 MTR_3g006150 42.276 123 42 2 2 98 9 128 1.63e-20 80.9
MS.gene71585.t1 MTR_1g109550 42.857 112 46 3 4 98 19 129 4.33e-20 79.3
MS.gene71585.t1 MTR_8g062350 50.667 75 37 0 2 76 15 89 8.07e-20 80.5
MS.gene71585.t1 MTR_1g104937 50.000 76 38 0 2 77 13 88 4.10e-19 76.3
MS.gene71585.t1 MTR_4g133300 42.609 115 43 2 2 96 13 124 6.69e-19 79.0
MS.gene71585.t1 MTR_4g133300 52.857 70 33 0 4 73 175 244 5.21e-18 76.6
MS.gene71585.t1 MTR_4g133300 38.655 119 50 1 2 97 90 208 4.08e-17 73.9
MS.gene71585.t1 MTR_3g006125 56.338 71 30 1 2 71 181 251 7.53e-19 79.0
MS.gene71585.t1 MTR_3g006125 39.370 127 45 2 2 96 90 216 9.71e-17 73.2
MS.gene71585.t1 MTR_1g104980 47.436 78 41 0 4 81 73 150 1.81e-18 76.3
MS.gene71585.t1 MTR_1g104980 39.048 105 53 1 3 96 1 105 9.07e-17 71.6
MS.gene71585.t1 MTR_7g089490 51.429 70 34 0 2 71 17 86 1.91e-18 74.7
MS.gene71585.t1 MTR_4g133470 42.609 115 43 2 2 96 13 124 1.95e-18 77.8
MS.gene71585.t1 MTR_4g133470 52.857 70 33 0 4 73 175 244 3.89e-18 76.6
MS.gene71585.t1 MTR_4g133470 36.975 119 52 1 2 97 90 208 1.04e-14 67.8
MS.gene71585.t1 MTR_7g013410 55.385 65 29 0 5 69 2 66 2.09e-18 75.1
MS.gene71585.t1 MTR_1g105010 43.590 78 44 0 2 79 17 94 4.62e-18 73.6
MS.gene71585.t1 MTR_2g097830 46.067 89 43 2 2 88 71 156 9.66e-18 73.9
MS.gene71585.t1 MTR_6g008995 64.815 54 17 1 23 76 2 53 9.48e-17 74.3
MS.gene71585.t1 MTR_3g006185 39.683 126 45 2 2 96 9 134 1.09e-16 71.6
MS.gene71585.t1 MTR_3g006185 51.429 70 33 1 2 70 99 168 2.09e-15 68.2
MS.gene71585.t1 MTR_1g093310 51.429 70 28 1 3 72 11 74 1.64e-16 68.6
MS.gene71585.t1 MTR_1g023820 74.359 39 10 0 31 69 17 55 2.14e-16 69.7
MS.gene71585.t1 MTR_3g006105 37.795 127 47 2 2 96 9 135 2.06e-15 68.6
MS.gene71585.t1 MTR_3g006105 50.000 70 34 1 2 70 100 169 2.59e-13 62.8
MS.gene71585.t1 MTR_1g091047 50.000 64 31 1 3 65 11 74 1.16e-14 63.9
MS.gene71585.t1 MTR_5g055670 60.000 50 19 1 20 68 74 123 2.45e-13 62.0
MS.gene71585.t1 MTR_3g006000 55.224 67 27 3 2 66 8 73 2.27e-12 58.2
MS.gene71585.t1 MTR_3g006010 51.471 68 32 1 2 68 9 76 4.20e-12 61.2
MS.gene71585.t1 MTR_7g013380 37.662 77 46 1 2 76 186 262 1.24e-11 59.7
MS.gene71585.t1 MTR_7g013380 29.231 130 57 3 15 109 115 244 4.11e-11 58.2
MS.gene71585.t1 MTR_7g083950 50.769 65 29 2 4 65 16 80 1.47e-11 59.7
MS.gene71585.t1 MTR_5g073430 46.154 78 37 4 2 74 12 89 1.81e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71585.t1 AT1G70060 69.333 75 23 0 2 76 15 89 8.76e-30 112
MS.gene71585.t1 AT1G70060 69.333 75 23 0 2 76 15 89 8.76e-30 112
MS.gene71585.t1 AT1G24190 69.863 73 22 0 2 74 15 87 1.96e-29 110
MS.gene71585.t1 AT1G24190 69.863 73 22 0 2 74 15 87 2.23e-29 110
MS.gene71585.t1 AT1G24190 69.863 73 22 0 2 74 15 87 2.31e-29 110
MS.gene71585.t1 AT3G01320 68.919 74 23 0 2 75 58 131 2.84e-28 107
MS.gene71585.t1 AT3G01320 68.919 74 23 0 2 75 58 131 3.01e-28 107
MS.gene71585.t1 AT5G15020 70.588 68 20 0 2 69 53 120 4.43e-27 104
MS.gene71585.t1 AT5G15020 70.588 68 20 0 2 69 53 120 4.65e-27 103
MS.gene71585.t1 AT1G59890 62.667 75 28 0 2 76 46 120 3.45e-26 101
MS.gene71585.t1 AT1G59890 62.667 75 28 0 2 76 46 120 3.55e-26 101
MS.gene71585.t1 AT1G59890 62.667 75 28 0 2 76 46 120 3.65e-26 101
MS.gene71585.t1 AT1G59890 63.514 74 27 0 2 75 46 119 4.10e-26 101
MS.gene71585.t1 AT1G10450 66.176 68 23 0 2 69 34 101 5.92e-25 98.2
MS.gene71585.t1 AT1G10450 66.176 68 23 0 2 69 34 101 5.92e-25 98.2
MS.gene71585.t1 AT1G10450 66.176 68 23 0 2 69 85 152 5.94e-25 98.2
MS.gene71585.t1 AT1G27250 53.521 71 33 0 2 72 22 92 3.95e-22 84.7
MS.gene71585.t1 AT1G70030 48.780 82 40 1 2 83 23 102 7.37e-22 83.6
MS.gene71585.t1 AT1G24200 57.353 68 29 0 2 69 15 82 1.19e-21 85.1
MS.gene71585.t1 AT1G70030 48.780 82 40 1 2 83 23 102 1.80e-21 83.2
MS.gene71585.t1 AT5G15025 55.128 78 32 1 2 79 48 122 2.62e-21 82.0
MS.gene71585.t1 AT1G27220 52.941 68 32 0 2 69 15 82 4.05e-21 83.2
MS.gene71585.t1 AT1G70030 48.780 82 40 1 2 83 12 91 5.21e-21 82.4
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.13e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.20e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.20e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.22e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.28e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.31e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.31e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 242 314 1.31e-20 85.9
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.38e-20 85.5
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.61e-20 85.5
MS.gene71585.t1 AT4G12020 55.405 74 32 1 2 75 309 381 1.61e-20 85.5
MS.gene71585.t1 AT5G15040 48.052 77 38 1 4 80 6 80 5.88e-19 75.1
MS.gene71585.t1 AT5G15040 48.052 77 38 1 4 80 6 80 5.88e-19 75.1
MS.gene71585.t1 AT5G15040 48.052 77 38 1 4 80 6 80 5.88e-19 75.1
MS.gene71585.t1 AT1G24250 42.857 91 48 1 2 88 121 211 1.54e-17 75.5
MS.gene71585.t1 AT5G35610 52.113 71 34 0 5 75 14 84 5.11e-17 72.0
MS.gene71585.t1 AT1G23810 40.659 91 50 1 2 88 110 200 8.41e-17 73.2
MS.gene71585.t1 AT1G24230 36.697 109 53 3 2 96 15 121 3.96e-15 68.9
MS.gene71585.t1 AT1G24230 34.211 76 50 0 4 79 89 164 1.31e-12 62.0
MS.gene71585.t1 AT1G27260 32.800 125 54 2 2 96 4 128 1.24e-13 64.3
MS.gene71585.t1 AT1G27260 32.967 91 57 1 2 88 94 184 2.21e-11 58.5
MS.gene71585.t1 AT1G24210 42.857 63 36 0 2 64 15 77 7.22e-12 58.5
MS.gene71585.t1 AT1G27280 42.857 63 36 0 2 64 82 144 9.62e-12 59.3

Find 15 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACTGATTTCATATCCTTT+TGG 0.331804 1.4:+81785887 None:intergenic
AGAGTGAAGGAGCTGTTTAA+AGG 0.388525 1.4:-81785947 MS.gene71585:CDS
AAATGTTATGAAAGAATTCA+AGG 0.402015 1.4:-81786002 MS.gene71585:CDS
GAAAGTTATGAGGGATTTCA+AGG 0.411631 1.4:-81785822 MS.gene71585:CDS
ACAGCTCCTTCACTCTTTCG+AGG 0.437658 1.4:+81785954 None:intergenic
GAGTGAAGGAGCTGTTTAAA+GGG 0.499135 1.4:-81785946 MS.gene71585:CDS
AAACTTTGATGATGATGAAA+GGG 0.500749 1.4:-81785864 MS.gene71585:CDS
TCTCGGAATTGACAATGAAA+TGG 0.509287 1.4:-81785798 MS.gene71585:CDS
TTCAACACCTTCCTGCCAAA+AGG 0.512524 1.4:-81785902 MS.gene71585:CDS
ATGAGGGATTTCAAGGCTCT+CGG 0.525498 1.4:-81785815 MS.gene71585:CDS
CAAACTTTGATGATGATGAA+AGG 0.533006 1.4:-81785865 MS.gene71585:CDS
AGTCGAGTTTCAAGATGAGA+TGG 0.542797 1.4:-81786044 MS.gene71585:CDS
TTATGAAAGAATTCAAGGCT+AGG 0.608401 1.4:-81785997 MS.gene71585:CDS
TGGTGTCCTCGAAAGAGTGA+AGG 0.637601 1.4:-81785960 MS.gene71585:CDS
GCTAGGAGAATTGATACAAG+TGG 0.644745 1.4:-81785980 MS.gene71585:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTTATGAAAGAATTCA+AGG - chr1.4:81785837-81785856 MS.gene71585:CDS 20.0%
!!! ATGAATTTTTGAAAGTTATG+AGG - chr1.4:81786007-81786026 MS.gene71585:CDS 20.0%
!!! TGAATTTTTGAAAGTTATGA+GGG - chr1.4:81786008-81786027 MS.gene71585:CDS 20.0%
! AGGGCATAAAAATTTAATCT+TGG - chr1.4:81785912-81785931 MS.gene71585:CDS 25.0%
! GGGCATAAAAATTTAATCTT+GGG - chr1.4:81785913-81785932 MS.gene71585:CDS 25.0%
!!! AAACTTTGATGATGATGAAA+GGG - chr1.4:81785975-81785994 MS.gene71585:CDS 25.0%
TTATGAAAGAATTCAAGGCT+AGG - chr1.4:81785842-81785861 MS.gene71585:CDS 30.0%
!! CAAACTTTGATGATGATGAA+AGG - chr1.4:81785974-81785993 MS.gene71585:CDS 30.0%
!!! GTACTGATTTCATATCCTTT+TGG + chr1.4:81785955-81785974 None:intergenic 30.0%
GAAAGTTATGAGGGATTTCA+AGG - chr1.4:81786017-81786036 MS.gene71585:CDS 35.0%
TCTCGGAATTGACAATGAAA+TGG - chr1.4:81786041-81786060 MS.gene71585:CDS 35.0%
! TGATTTCATATCCTTTTGGC+AGG + chr1.4:81785951-81785970 None:intergenic 35.0%
AGAGTGAAGGAGCTGTTTAA+AGG - chr1.4:81785892-81785911 MS.gene71585:CDS 40.0%
AGTCGAGTTTCAAGATGAGA+TGG - chr1.4:81785795-81785814 MS.gene71585:CDS 40.0%
GAGTGAAGGAGCTGTTTAAA+GGG - chr1.4:81785893-81785912 MS.gene71585:CDS 40.0%
GCTAGGAGAATTGATACAAG+TGG - chr1.4:81785859-81785878 MS.gene71585:CDS 40.0%
! TTCATATCCTTTTGGCAGGA+AGG + chr1.4:81785947-81785966 None:intergenic 40.0%
ATGAGGGATTTCAAGGCTCT+CGG - chr1.4:81786024-81786043 MS.gene71585:CDS 45.0%
TTCAACACCTTCCTGCCAAA+AGG - chr1.4:81785937-81785956 MS.gene71585:CDS 45.0%
ACAGCTCCTTCACTCTTTCG+AGG + chr1.4:81785888-81785907 None:intergenic 50.0%
TGGTGTCCTCGAAAGAGTGA+AGG - chr1.4:81785879-81785898 MS.gene71585:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 81785766 81786095 81785766 ID=MS.gene71585
chr1.4 mRNA 81785766 81786095 81785766 ID=MS.gene71585.t1;Parent=MS.gene71585
chr1.4 exon 81785766 81786095 81785766 ID=MS.gene71585.t1.exon1;Parent=MS.gene71585.t1
chr1.4 CDS 81785766 81786095 81785766 ID=cds.MS.gene71585.t1;Parent=MS.gene71585.t1
Gene Sequence

>MS.gene71585

ATGGCACTAGCATATCTGAAAGCAGTAAGAGTCGAGTTTCAAGATGAGATGGAAAAGTATGATGAATTTTTAAATGTTATGAAAGAATTCAAGGCTAGGAGAATTGATACAAGTGGTGTCCTCGAAAGAGTGAAGGAGCTGTTTAAAGGGCATAAAAATTTAATCTTGGGATTCAACACCTTCCTGCCAAAAGGATATGAAATCAGTACAAACTTTGATGATGATGAAAGGGAAAATTATGATGAATTTTTGAAAGTTATGAGGGATTTCAAGGCTCTCGGAATTGACAATGAAATGGAGCAGATTGTTCAACTTTTGTCACGTCTATGA
Protein sequence

>MS.gene71585.t1

MALAYLKAVRVEFQDEMEKYDEFLNVMKEFKARRIDTSGVLERVKELFKGHKNLILGFNTFLPKGYEISTNFDDDERENYDEFLKVMRDFKALGIDNEMEQIVQLLSRL