AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71832


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71832.t1 MTR_1g110840 95.671 231 10 0 1 231 1 231 4.43e-167 460
MS.gene71832.t1 MTR_6g453130 93.939 231 14 0 1 231 1 231 1.77e-164 453
MS.gene71832.t1 MTR_6g054080 90.476 231 22 0 1 231 1 231 2.35e-158 438
MS.gene71832.t1 MTR_6g054150 88.745 231 26 0 1 231 1 231 2.96e-156 432
MS.gene71832.t1 MTR_6g018270 55.172 174 57 7 1 168 1 159 6.24e-62 195
MS.gene71832.t1 MTR_7g007010 44.053 227 109 7 1 223 1 213 1.07e-55 180
MS.gene71832.t1 MTR_4g095600 56.303 119 48 2 3 119 1 117 2.10e-46 154
MS.gene71832.t1 MTR_5g015880 44.970 169 82 5 3 165 1 164 7.00e-45 150
MS.gene71832.t1 MTR_1g106420 44.882 127 63 2 3 124 1 125 1.02e-35 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71832.t1 AT3G02550 40.529 227 122 5 1 225 1 216 2.70e-53 172
MS.gene71832.t1 AT1G68510 55.102 147 54 3 1 145 1 137 2.97e-53 171
MS.gene71832.t1 AT1G67100 54.861 144 57 3 1 144 1 136 5.76e-50 163
MS.gene71832.t1 AT3G49940 53.659 123 55 1 3 125 1 121 1.37e-45 152
MS.gene71832.t1 AT5G67420 55.963 109 46 1 3 111 1 107 1.10e-43 147
MS.gene71832.t1 AT5G67420 55.963 109 46 1 3 111 1 107 1.10e-43 147
MS.gene71832.t1 AT4G37540 57.798 109 44 1 3 111 1 107 1.46e-43 147

Find 62 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCATGCCCTTGGGATTC+AGG 0.157225 1.3:-75557827 None:intergenic
ATGGGGCGCTTGGCTTATTT+TGG 0.165756 1.3:+75558303 MS.gene71832:CDS
AAGAATACCAACATGGAAAT+TGG 0.166066 1.3:+75558602 MS.gene71832:CDS
ACCGGTTCGGCCATAAAATT+TGG 0.172606 1.3:-75557861 None:intergenic
ATGGCGTATATCATAGGTTT+TGG 0.254539 1.3:-75558456 None:intergenic
TGCTGAGAATCAAGTGAATT+TGG 0.279066 1.3:+75558667 MS.gene71832:CDS
CCATGTTGGTATTCTTCAAA+AGG 0.367139 1.3:-75558595 None:intergenic
AGAATACCAACATGGAAATT+GGG 0.374565 1.3:+75558603 MS.gene71832:CDS
TGCCACATGGCGTATATCAT+AGG 0.386203 1.3:-75558462 None:intergenic
CTTTGATTGCCAATCAACTA+AGG 0.392207 1.3:+75558703 MS.gene71832:CDS
CATAAAATTTGGCAAGGAAG+AGG 0.404166 1.3:-75557850 None:intergenic
GACTGAACCAACTCGTGGCT+TGG 0.415502 1.3:-75558549 None:intergenic
ACTGAACCAACTCGTGGCTT+GGG 0.424285 1.3:-75558548 None:intergenic
GTGAATTTGGAACTCACTCT+TGG 0.424885 1.3:+75558680 MS.gene71832:CDS
GGAAGAGGGTGGCATGCCCT+TGG 0.443110 1.3:-75557835 None:intergenic
GCAACTGATCCACTCAAGGC+AGG 0.443950 1.3:-75557804 None:intergenic
TGGCATGCCCTTGGGATTCA+GGG 0.444879 1.3:-75557826 None:intergenic
AAAACTCAATTCAAAAGAGC+TGG 0.446487 1.3:+75558512 MS.gene71832:CDS
ATTGATGGGCAGACATCATC+AGG 0.455052 1.3:+75558404 MS.gene71832:CDS
GCAGTTGAAGCTGTGCTGAC+TGG 0.456964 1.3:+75558353 MS.gene71832:CDS
AGATTGGTGAATCCCACATA+TGG 0.458590 1.3:+75558284 MS.gene71832:CDS
TAAGCCAAGCGCCCCATATG+TGG 0.471835 1.3:-75558297 None:intergenic
CATGTTGGTATTCTTCAAAA+GGG 0.477135 1.3:-75558594 None:intergenic
GAAGAGGGTGGCATGCCCTT+GGG 0.479104 1.3:-75557834 None:intergenic
GGAGATTGAGCAAACCGGTT+CGG 0.494518 1.3:-75557874 None:intergenic
AATCATCACTACAACCTTTG+CGG 0.504650 1.3:-75557769 None:intergenic
GATTGGTGAATCCCACATAT+GGG 0.508471 1.3:+75558285 MS.gene71832:CDS
GATATACGCCATGTGGCAAA+AGG 0.516720 1.3:+75558467 MS.gene71832:CDS
AATCCCACATATGGGGCGCT+TGG 0.518707 1.3:+75558293 MS.gene71832:CDS
GGTGAGGAGATTGAGCAAAC+CGG 0.524508 1.3:-75557879 None:intergenic
TCATCAAGCCCTGCCTTGAG+TGG 0.538225 1.3:+75557795 MS.gene71832:CDS
GTACCAATGTGGACATCAAA+GGG 0.546635 1.3:+75558489 MS.gene71832:CDS
TTTCCCTTTGATGTCCACAT+TGG 0.551024 1.3:-75558492 None:intergenic
GGTACCAATGTGGACATCAA+AGG 0.551224 1.3:+75558488 MS.gene71832:CDS
GTACGAGGCAGCTGGTAGAT+TGG 0.560594 1.3:+75558268 MS.gene71832:CDS
GAATACCAACATGGAAATTG+GGG 0.561319 1.3:+75558604 MS.gene71832:CDS
CTGAAGCCCAAGCCACGAGT+TGG 0.576033 1.3:+75558542 MS.gene71832:CDS
AGTCTCCCCAATTTCCATGT+TGG 0.576332 1.3:-75558609 None:intergenic
ATAAAATTTGGCAAGGAAGA+GGG 0.578936 1.3:-75557849 None:intergenic
TTCGGCCATAAAATTTGGCA+AGG 0.579687 1.3:-75557856 None:intergenic
TAATGAAGTTGAGTTGCAAT+GGG 0.583194 1.3:+75557735 None:intergenic
TTTGATTGCCAATCAACTAA+GGG 0.586746 1.3:+75558704 MS.gene71832:CDS
AAGCCAAGCGCCCCATATGT+GGG 0.589682 1.3:-75558296 None:intergenic
AAACTCAATTCAAAAGAGCT+GGG 0.590181 1.3:+75558513 MS.gene71832:CDS
CAACTGATCCACTCAAGGCA+GGG 0.595554 1.3:-75557803 None:intergenic
ATCAGTTGCCCTGAATCCCA+AGG 0.599742 1.3:+75557818 MS.gene71832:CDS
ATAATGAAGTTGAGTTGCAA+TGG 0.608858 1.3:+75557734 None:intergenic
TCATTGTTGTACGAGGCAGC+TGG 0.614180 1.3:+75558260 MS.gene71832:CDS
ACGAGTTGGTTCAGTCGACT+CGG 0.622355 1.3:+75558556 MS.gene71832:CDS
CAGGGCAACTGATCCACTCA+AGG 0.625744 1.3:-75557808 None:intergenic
GGGTGTCGTGTGCTCCGCAA+AGG 0.627575 1.3:+75557755 MS.gene71832:CDS
ATTGGTGAATCCCACATATG+GGG 0.640018 1.3:+75558286 MS.gene71832:CDS
TTTGATTGGTAGCATTGGTG+AGG 0.643249 1.3:-75557895 None:intergenic
AAATTGGGGAGACTTCATCA+AGG 0.644743 1.3:+75558618 MS.gene71832:CDS
AATTGGGGAGACTTCATCAA+GGG 0.644822 1.3:+75558619 MS.gene71832:CDS
AATCAAAATCATAATCCACC+AGG 0.664735 1.3:+75557911 MS.gene71832:CDS
TCAGTTGCCCTGAATCCCAA+GGG 0.669183 1.3:+75557819 MS.gene71832:CDS
GAGTCGACTGAACCAACTCG+TGG 0.679210 1.3:-75558554 None:intergenic
AACCTATGATATACGCCATG+TGG 0.679816 1.3:+75558460 MS.gene71832:CDS
AAATTTGGCAAGGAAGAGGG+TGG 0.680062 1.3:-75557846 None:intergenic
GTTTAAATCATTGTTGTACG+AGG 0.684324 1.3:+75558253 MS.gene71832:CDS
TGTGGCAAAAGGTACCAATG+TGG 0.731996 1.3:+75558478 MS.gene71832:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAATTCATGAATATTGA+TGG + chr1.3:75557965-75557984 MS.gene71832:intron 20.0%
!!! GAATCTATGAAAAGATTTTA+TGG - chr1.3:75558161-75558180 None:intergenic 20.0%
! AATTCATGAATATTGATGGA+TGG + chr1.3:75557969-75557988 MS.gene71832:intron 25.0%
!! ACAGTTTTACAATGATTGAT+GGG + chr1.3:75558390-75558409 MS.gene71832:CDS 25.0%
!! GTTTAATTTATGATCCGTAA+CGG - chr1.3:75558119-75558138 None:intergenic 25.0%
AAAACTCAATTCAAAAGAGC+TGG + chr1.3:75558512-75558531 MS.gene71832:CDS 30.0%
AAACTCAATTCAAAAGAGCT+GGG + chr1.3:75558513-75558532 MS.gene71832:CDS 30.0%
AAGAATACCAACATGGAAAT+TGG + chr1.3:75558602-75558621 MS.gene71832:CDS 30.0%
AATCAAAATCATAATCCACC+AGG + chr1.3:75557911-75557930 MS.gene71832:CDS 30.0%
AGAATACCAACATGGAAATT+GGG + chr1.3:75558603-75558622 MS.gene71832:CDS 30.0%
ATAAAATTTGGCAAGGAAGA+GGG - chr1.3:75557852-75557871 None:intergenic 30.0%
CATGTTGGTATTCTTCAAAA+GGG - chr1.3:75558597-75558616 None:intergenic 30.0%
GTTTAAATCATTGTTGTACG+AGG + chr1.3:75558253-75558272 MS.gene71832:CDS 30.0%
TAAAAAAGTTTGTCGTGTAG+CGG - chr1.3:75558051-75558070 None:intergenic 30.0%
TGGAACATAAATGAGACATT+AGG - chr1.3:75558141-75558160 None:intergenic 30.0%
TTTGATTGCCAATCAACTAA+GGG + chr1.3:75558704-75558723 MS.gene71832:CDS 30.0%
! ACCACAATTTAAAACCATGA+TGG - chr1.3:75558017-75558036 None:intergenic 30.0%
! CAATTTAAAACCATGATGGA+TGG - chr1.3:75558013-75558032 None:intergenic 30.0%
! GACAGTTTTACAATGATTGA+TGG + chr1.3:75558389-75558408 MS.gene71832:CDS 30.0%
!!! ATGATTTTGATTGGTAGCAT+TGG - chr1.3:75557903-75557922 None:intergenic 30.0%
!!! TCCATCATGGTTTTAAATTG+TGG + chr1.3:75558013-75558032 MS.gene71832:intron 30.0%
!!! TGGTGGATTATGATTTTGAT+TGG - chr1.3:75557912-75557931 None:intergenic 30.0%
AATCATCACTACAACCTTTG+CGG - chr1.3:75557772-75557791 None:intergenic 35.0%
ATCAATGATTCCATCCATCA+TGG + chr1.3:75558000-75558019 MS.gene71832:intron 35.0%
CATAAAATTTGGCAAGGAAG+AGG - chr1.3:75557853-75557872 None:intergenic 35.0%
CCATGTTGGTATTCTTCAAA+AGG - chr1.3:75558598-75558617 None:intergenic 35.0%
CTTTGATTGCCAATCAACTA+AGG + chr1.3:75558703-75558722 MS.gene71832:CDS 35.0%
GAATACCAACATGGAAATTG+GGG + chr1.3:75558604-75558623 MS.gene71832:CDS 35.0%
TGCTGAGAATCAAGTGAATT+TGG + chr1.3:75558667-75558686 MS.gene71832:CDS 35.0%
! ATGGCGTATATCATAGGTTT+TGG - chr1.3:75558459-75558478 None:intergenic 35.0%
! CTCTTCCTTGCCAAATTTTA+TGG + chr1.3:75557851-75557870 MS.gene71832:CDS 35.0%
! TGGCGTATATCATAGGTTTT+GGG - chr1.3:75558458-75558477 None:intergenic 35.0%
! TTTTCTTGCCCTTAGTTGAT+TGG - chr1.3:75558715-75558734 None:intergenic 35.0%
!! CCTTTTGAAGAATACCAACA+TGG + chr1.3:75558595-75558614 MS.gene71832:CDS 35.0%
AAATTGGGGAGACTTCATCA+AGG + chr1.3:75558618-75558637 MS.gene71832:CDS 40.0%
AACCTATGATATACGCCATG+TGG + chr1.3:75558460-75558479 MS.gene71832:CDS 40.0%
AATTGGGGAGACTTCATCAA+GGG + chr1.3:75558619-75558638 MS.gene71832:CDS 40.0%
AGATTGGTGAATCCCACATA+TGG + chr1.3:75558284-75558303 MS.gene71832:CDS 40.0%
ATTGGTGAATCCCACATATG+GGG + chr1.3:75558286-75558305 MS.gene71832:CDS 40.0%
GATTGGTGAATCCCACATAT+GGG + chr1.3:75558285-75558304 MS.gene71832:CDS 40.0%
GTACCAATGTGGACATCAAA+GGG + chr1.3:75558489-75558508 MS.gene71832:CDS 40.0%
TATGATCCGTAACGGTATTG+CGG - chr1.3:75558111-75558130 None:intergenic 40.0%
TTCGGCCATAAAATTTGGCA+AGG - chr1.3:75557859-75557878 None:intergenic 40.0%
TTTCCCTTTGATGTCCACAT+TGG - chr1.3:75558495-75558514 None:intergenic 40.0%
! GGCGTATATCATAGGTTTTG+GGG - chr1.3:75558457-75558476 None:intergenic 40.0%
! GTGAATTTGGAACTCACTCT+TGG + chr1.3:75558680-75558699 MS.gene71832:CDS 40.0%
!! GTGACCAACTTAAGTTGATG+CGG + chr1.3:75558085-75558104 MS.gene71832:intron 40.0%
!! TTTGATTGGTAGCATTGGTG+AGG - chr1.3:75557898-75557917 None:intergenic 40.0%
!!! GCTTATTTTGGACAGCGAAT+TGG + chr1.3:75558315-75558334 MS.gene71832:CDS 40.0%
AAATTTGGCAAGGAAGAGGG+TGG - chr1.3:75557849-75557868 None:intergenic 45.0%
AGTCTCCCCAATTTCCATGT+TGG - chr1.3:75558612-75558631 None:intergenic 45.0%
AGTGAACATAGCTAGCTACC+TGG - chr1.3:75557932-75557951 None:intergenic 45.0%
GATATACGCCATGTGGCAAA+AGG + chr1.3:75558467-75558486 MS.gene71832:CDS 45.0%
GGTACCAATGTGGACATCAA+AGG + chr1.3:75558488-75558507 MS.gene71832:CDS 45.0%
TGCCACATGGCGTATATCAT+AGG - chr1.3:75558465-75558484 None:intergenic 45.0%
! ATTGATGGGCAGACATCATC+AGG + chr1.3:75558404-75558423 MS.gene71832:CDS 45.0%
! CATTGGTACCTTTTGCCACA+TGG - chr1.3:75558478-75558497 None:intergenic 45.0%
! GCCAAATTTTATGGCCGAAC+CGG + chr1.3:75557860-75557879 MS.gene71832:CDS 45.0%
!! ACCGGTTCGGCCATAAAATT+TGG - chr1.3:75557864-75557883 None:intergenic 45.0%
!! TGTGGCAAAAGGTACCAATG+TGG + chr1.3:75558478-75558497 MS.gene71832:CDS 45.0%
ACTGAACCAACTCGTGGCTT+GGG - chr1.3:75558551-75558570 None:intergenic 50.0%
ATCAGTTGCCCTGAATCCCA+AGG + chr1.3:75557818-75557837 MS.gene71832:CDS 50.0%
CAACTGATCCACTCAAGGCA+GGG - chr1.3:75557806-75557825 None:intergenic 50.0%
GAACATAGCTAGCTACCTGG+TGG - chr1.3:75557929-75557948 None:intergenic 50.0%
GGAGATTGAGCAAACCGGTT+CGG - chr1.3:75557877-75557896 None:intergenic 50.0%
GGTGAGGAGATTGAGCAAAC+CGG - chr1.3:75557882-75557901 None:intergenic 50.0%
TCAGTTGCCCTGAATCCCAA+GGG + chr1.3:75557819-75557838 MS.gene71832:CDS 50.0%
TCATTGTTGTACGAGGCAGC+TGG + chr1.3:75558260-75558279 MS.gene71832:CDS 50.0%
!! ACGAGTTGGTTCAGTCGACT+CGG + chr1.3:75558556-75558575 MS.gene71832:CDS 50.0%
!! ATGGGGCGCTTGGCTTATTT+TGG + chr1.3:75558303-75558322 MS.gene71832:CDS 50.0%
AAGCCAAGCGCCCCATATGT+GGG - chr1.3:75558299-75558318 None:intergenic 55.0%
ATGCGGCCGCAATACCGTTA+CGG + chr1.3:75558102-75558121 MS.gene71832:intron 55.0%
CAGGGCAACTGATCCACTCA+AGG - chr1.3:75557811-75557830 None:intergenic 55.0%
GACTGAACCAACTCGTGGCT+TGG - chr1.3:75558552-75558571 None:intergenic 55.0%
GAGTCGACTGAACCAACTCG+TGG - chr1.3:75558557-75558576 None:intergenic 55.0%
GCAACTGATCCACTCAAGGC+AGG - chr1.3:75557807-75557826 None:intergenic 55.0%
GCGGCCGCATCAACTTAAGT+TGG - chr1.3:75558092-75558111 None:intergenic 55.0%
GTACGAGGCAGCTGGTAGAT+TGG + chr1.3:75558268-75558287 MS.gene71832:CDS 55.0%
TAAGCCAAGCGCCCCATATG+TGG - chr1.3:75558300-75558319 None:intergenic 55.0%
TCATCAAGCCCTGCCTTGAG+TGG + chr1.3:75557795-75557814 MS.gene71832:CDS 55.0%
TGGCATGCCCTTGGGATTCA+GGG - chr1.3:75557829-75557848 None:intergenic 55.0%
! AATCCCACATATGGGGCGCT+TGG + chr1.3:75558293-75558312 MS.gene71832:CDS 55.0%
! GCAGTTGAAGCTGTGCTGAC+TGG + chr1.3:75558353-75558372 MS.gene71832:CDS 55.0%
CTGAAGCCCAAGCCACGAGT+TGG + chr1.3:75558542-75558561 MS.gene71832:CDS 60.0%
GAAGAGGGTGGCATGCCCTT+GGG - chr1.3:75557837-75557856 None:intergenic 60.0%
GTGGCATGCCCTTGGGATTC+AGG - chr1.3:75557830-75557849 None:intergenic 60.0%
GGAAGAGGGTGGCATGCCCT+TGG - chr1.3:75557838-75557857 None:intergenic 65.0%
! GGGTGTCGTGTGCTCCGCAA+AGG + chr1.3:75557755-75557774 MS.gene71832:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 75557737 75558748 75557737 ID=MS.gene71832
chr1.3 mRNA 75557737 75558748 75557737 ID=MS.gene71832.t1;Parent=MS.gene71832
chr1.3 exon 75557737 75557932 75557737 ID=MS.gene71832.t1.exon1;Parent=MS.gene71832.t1
chr1.3 CDS 75557737 75557932 75557737 ID=cds.MS.gene71832.t1;Parent=MS.gene71832.t1
chr1.3 exon 75558249 75558748 75558249 ID=MS.gene71832.t1.exon2;Parent=MS.gene71832.t1
chr1.3 CDS 75558249 75558748 75558249 ID=cds.MS.gene71832.t1;Parent=MS.gene71832.t1
Gene Sequence

>MS.gene71832

ATGAAGTTGAGTTGCAATGGGTGTCGTGTGCTCCGCAAAGGTTGTAGTGATGATTGTATCATCAAGCCCTGCCTTGAGTGGATCAGTTGCCCTGAATCCCAAGGGCATGCCACCCTCTTCCTTGCCAAATTTTATGGCCGAACCGGTTTGCTCAATCTCCTCACCAATGCTACCAATCAAAATCATAATCCACCAGGTAGCTAGCTATGTTCACTATCATAATCTAACACTTAATTCATGAATATTGATGGATGGAATGATTGATCAATGATTCCATCCATCATGGTTTTAAATTGTGGTTGTTGCAGTTGCCGCTACACGACAAACTTTTTTAATGTAATAATAAATGTGACCAACTTAAGTTGATGCGGCCGCAATACCGTTACGGATCATAAATTAAACCTAATGTCTCATTTATGTTCCATAAAATCTTTTCATAGATTCTACATCAAATTTGATTTTAGTTTTTAATATATGATGATTAATGTCTTTAATTTTGTCACAATTTTCAGCTGTGTTTAAATCATTGTTGTACGAGGCAGCTGGTAGATTGGTGAATCCCACATATGGGGCGCTTGGCTTATTTTGGACAGCGAATTGGAGTCGTTGTGAAGCTGCAGTTGAAGCTGTGCTGACTGGATCGAATATTAATGACAGTTTTACAATGATTGATGGGCAGACATCATCAGGTACACTCAATGCTGAAAATCATGTTCTCCCCAAAACCTATGATATACGCCATGTGGCAAAAGGTACCAATGTGGACATCAAAGGGAAAACTCAATTCAAAAGAGCTGGGCAAGTTCTGAAGCCCAAGCCACGAGTTGGTTCAGTCGACTCGGCTACAATGTTGAAATCCCTTTTGAAGAATACCAACATGGAAATTGGGGAGACTTCATCAAGGGTTCAAACAGAGAAAATTAATGAAGCTGCTGAGAATCAAGTGAATTTGGAACTCACTCTTGGCTTTGATTGCCAATCAACTAAGGGCAAGAAAATATTAGATAAATGA

Protein sequence

>MS.gene71832.t1

MKLSCNGCRVLRKGCSDDCIIKPCLEWISCPESQGHATLFLAKFYGRTGLLNLLTNATNQNHNPPAVFKSLLYEAAGRLVNPTYGALGLFWTANWSRCEAAVEAVLTGSNINDSFTMIDGQTSSGTLNAENHVLPKTYDIRHVAKGTNVDIKGKTQFKRAGQVLKPKPRVGSVDSATMLKSLLKNTNMEIGETSSRVQTEKINEAAENQVNLELTLGFDCQSTKGKKILDK