AlfalfaGEDB | MS.gene71880

M. sativa cultivar XinJiangDaYe / MS.gene71880

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03110.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71880.t1	XP_013464662.1	95.6	68	3	0	5	72	104	171	2.70E-28	134

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71880.t1	Q9S7A9	66.0	47	15	1	5	51	100	145	1.0e-07	57.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71880.t1	A0A072VA58	95.6	68	3	0	5	72	104	171	1.9e-28	134.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene71880.t1	TF	SBP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71880.t1	MTR_2g078770	95.588	68	3	0	5	72	104	171	6.09e-43	136
MS.gene71880.t1	MTR_8g463140	55.714	70	29	1	5	72	111	180	1.35e-17	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71880.t1	AT1G53160	65.957	47	15	1	5	51	100	145	1.19e-12	59.7
MS.gene71880.t1	AT1G53160	65.957	47	15	1	5	51	100	145	1.22e-12	59.7
MS.gene71880.t1	AT1G53160	65.957	47	15	1	5	51	100	145	1.22e-12	59.7
MS.gene71880.t1	AT3G15270	74.359	39	10	0	5	43	109	147	2.79e-12	58.5
MS.gene71880.t1	AT2G33810	86.667	30	4	0	5	34	100	129	5.78e-11	54.3

Find 9 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGAACATAACAAGTAGTTC+TGG	0.412406	2.2:+15833347	MS.gene71880:CDS
AGCAAAATCATGATGGTGAT+TGG	0.426111	2.2:+15833312	MS.gene71880:CDS
TGCACTGAAGGAAGCGGTAC+TGG	0.443322	2.2:+15833287	MS.gene71880:CDS
CGGCGTCGTAAAAGCACTGC+TGG	0.466987	2.2:+15833260	MS.gene71880:CDS
ACTGCTGGAACTTGCACTGA+AGG	0.529046	2.2:+15833275	MS.gene71880:CDS
AACCTTTGATGAGTGTGCGC+AGG	0.591425	2.2:+15833168	None:intergenic
GGAACTTGCACTGAAGGAAG+CGG	0.610870	2.2:+15833281	MS.gene71880:CDS
ACTGGCCAGCAAAATCATGA+TGG	0.639807	2.2:+15833305	MS.gene71880:CDS
GCTTAGCGCGACACAATGAA+CGG	0.657384	2.2:+15833240	MS.gene71880:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGAACATAACAAGTAGTTC+TGG	+	chr2.2:15833347-15833366	MS.gene71880:CDS	30.0%
!	AATCACCATCATGATTTTGC+TGG	-	chr2.2:15833313-15833332	None:intergenic	35.0%
!	AGCAAAATCATGATGGTGAT+TGG	+	chr2.2:15833312-15833331	MS.gene71880:CDS	35.0%
	ACTGGCCAGCAAAATCATGA+TGG	+	chr2.2:15833305-15833324	MS.gene71880:CDS	45.0%
	ACTGCTGGAACTTGCACTGA+AGG	+	chr2.2:15833275-15833294	MS.gene71880:CDS	50.0%
	GCTTAGCGCGACACAATGAA+CGG	+	chr2.2:15833240-15833259	MS.gene71880:CDS	50.0%
	GGAACTTGCACTGAAGGAAG+CGG	+	chr2.2:15833281-15833300	MS.gene71880:CDS	50.0%
	TGCACTGAAGGAAGCGGTAC+TGG	+	chr2.2:15833287-15833306	MS.gene71880:CDS	55.0%
!!	CGGCGTCGTAAAAGCACTGC+TGG	+	chr2.2:15833260-15833279	MS.gene71880:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	15833176	15833394	15833176	ID=MS.gene71880
chr2.2	mRNA	15833176	15833394	15833176	ID=MS.gene71880.t1;Parent=MS.gene71880
chr2.2	exon	15833176	15833394	15833176	ID=MS.gene71880.t1.exon1;Parent=MS.gene71880.t1
chr2.2	CDS	15833176	15833394	15833176	ID=cds.MS.gene71880.t1;Parent=MS.gene71880.t1

Gene Sequence	>MS.gene71880 ATGAGTGTGCGCAGGTTTCATGAGCTTGCAGAGTTTGATGAATCGAAAAGAAGCTGTCGAAGACGCTTAGCGCGACACAATGAACGGCGTCGTAAAAGCACTGCTGGAACTTGCACTGAAGGAAGCGGTACTGGCCAGCAAAATCATGATGGTGATTGGAGACAAATTCATATGAACATAACAAGTAGTTCTGGTCACGATTTTCTGAATTTCAGATGA
Protein sequence	>MS.gene71880.t1 MSVRRFHELAEFDESKRSCRRRLARHNERRRKSTAGTCTEGSGTGQQNHDGDWRQIHMNITSSSGHDFLNFR

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