AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72173

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72173.t1 MTR_8g097300 98.104 211 4 0 1 211 1 211 6.53e-155 427
MS.gene72173.t1 MTR_3g070050 60.656 183 64 1 1 175 1 183 3.48e-76 228
MS.gene72173.t1 MTR_8g031490 59.538 173 68 1 1 171 1 173 1.07e-73 222
MS.gene72173.t1 MTR_7g076720 53.302 212 95 2 1 208 1 212 1.07e-72 219
MS.gene72173.t1 MTR_1g017950 55.367 177 67 4 1 167 1 175 2.13e-61 190
MS.gene72173.t1 MTR_4g106800 52.000 175 77 2 2 169 3 177 6.29e-61 188
MS.gene72173.t1 MTR_4g106800 50.704 71 35 0 93 163 2 72 4.23e-18 78.6
MS.gene72173.t1 MTR_3g070050 56.738 141 53 1 43 175 1 141 2.36e-51 163
MS.gene72173.t1 MTR_3g070050 42.424 66 38 0 3 68 61 126 2.88e-15 70.5
MS.gene72173.t1 MTR_3g070033 67.213 61 20 0 115 175 6 66 6.32e-25 93.2
MS.gene72173.t1 MTR_1g106100 51.471 68 33 0 92 159 57 124 2.89e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72173.t1 AT3G61230 54.930 213 87 3 2 210 3 210 2.67e-79 236
MS.gene72173.t1 AT3G61230 53.425 73 34 0 93 165 2 74 1.32e-21 89.0
MS.gene72173.t1 AT2G45800 56.250 208 85 3 1 202 1 208 1.73e-77 232
MS.gene72173.t1 AT2G39900 61.538 182 63 2 1 175 1 182 4.06e-77 230
MS.gene72173.t1 AT3G55770 57.286 199 79 1 1 193 1 199 1.63e-75 226
MS.gene72173.t1 AT3G55770 57.286 199 79 1 1 193 1 199 1.63e-75 226
MS.gene72173.t1 AT3G55770 57.286 199 79 1 1 193 1 199 1.63e-75 226
MS.gene72173.t1 AT3G55770 57.286 199 79 1 1 193 1 199 1.63e-75 226
MS.gene72173.t1 AT1G01780 53.659 205 88 2 1 203 1 200 3.25e-74 223
MS.gene72173.t1 AT3G55770 48.927 233 79 2 1 193 1 233 5.39e-68 208
MS.gene72173.t1 AT1G10200 54.802 177 68 4 1 167 1 175 1.62e-61 190
MS.gene72173.t1 AT2G45800 54.217 166 70 3 43 202 12 177 3.58e-53 169
MS.gene72173.t1 AT2G45800 48.438 64 33 0 3 66 68 131 5.39e-15 70.9
MS.gene72173.t1 AT3G55770 53.906 128 53 1 1 122 1 128 4.17e-42 139
MS.gene72173.t1 AT3G55770 46.392 97 41 1 95 191 3 88 2.96e-23 91.3
MS.gene72173.t1 AT3G55770 54.098 122 50 1 1 116 1 122 4.02e-39 131
MS.gene72173.t1 AT3G55770 46.392 97 41 1 95 191 3 88 2.22e-23 91.3
MS.gene72173.t1 AT1G10200 51.145 131 54 3 46 167 4 133 1.31e-37 128
MS.gene72173.t1 AT1G10200 45.455 66 36 0 3 68 61 126 7.54e-15 69.3

Find 47 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTATGCTGCTCTTGA+TGG 0.220465 4.2:+6831360 MS.gene72173:CDS
ATTACCCGTCTGGGGTTTCT+TGG 0.257758 4.2:-6830603 None:intergenic
AAACTTTCTGCTTTCTTCTC+AGG 0.328680 4.2:+6830892 MS.gene72173:CDS
TTTACCACAAGTCATGCTTT+AGG 0.384207 4.2:+6831307 MS.gene72173:CDS
CACAAGCCTTGCATTTCTGT+TGG 0.387121 4.2:-6829974 None:intergenic
ACAAGCCTTGCATTTCTGTT+GGG 0.412168 4.2:-6829973 None:intergenic
AAAGGATCTTATTACCCGTC+TGG 0.436417 4.2:-6830613 None:intergenic
TCTGTCTCTGTGTCTGATGC+TGG 0.438805 4.2:-6831516 None:intergenic
TCTCAGTTGTTTAAGGCAAA+AGG 0.442224 4.2:+6831408 MS.gene72173:CDS
ACCCGTCTGGGGTTTCTTGG+AGG 0.447284 4.2:-6830600 None:intergenic
AGAAAGCAGAAAGTTTGCTT+GGG 0.452697 4.2:-6830885 None:intergenic
GAAAGTTTGCTTGGGGCCCT+AGG 0.452962 4.2:-6830877 None:intergenic
AGCAGGAAAGCCTAGTGAAC+TGG 0.468952 4.2:+6830744 MS.gene72173:intron
AACTTTCTGCTTTCTTCTCA+GGG 0.473345 4.2:+6830893 MS.gene72173:CDS
TCAATAACCATGTCTTTCAC+TGG 0.477166 4.2:+6829948 None:intergenic
CAAGAAAACTGTATATCCAT+TGG 0.479463 4.2:+6830939 MS.gene72173:CDS
AAGAAAGCAGAAAGTTTGCT+TGG 0.480734 4.2:-6830886 None:intergenic
AAGGATCTTATTACCCGTCT+GGG 0.489662 4.2:-6830612 None:intergenic
AACTGTATATCCATTGGAAA+AGG 0.494668 4.2:+6830945 MS.gene72173:CDS
TCAAGAGCAGCATAGGATGA+AGG 0.519729 4.2:-6831357 None:intergenic
CTGGGGTTTCTTGGAGGCGA+AGG 0.523891 4.2:-6830594 None:intergenic
CTTAAACAACTGAGAAAAGT+GGG 0.527258 4.2:-6831400 None:intergenic
GAATCCATCAAGAGCAGCAT+AGG 0.537698 4.2:-6831364 None:intergenic
GTTGATACGTTATCTGCTGA+TGG 0.547689 4.2:+6830014 MS.gene72173:CDS
AGAATACACACCAGTTCACT+AGG 0.547764 4.2:-6830754 None:intergenic
CCTTAAACAACTGAGAAAAG+TGG 0.548044 4.2:-6831401 None:intergenic
TCATGCTTTAGGTGCACACA+TGG 0.562402 4.2:+6831318 MS.gene72173:CDS
TACACCTCTTGTGTAACAGC+AGG 0.567186 4.2:-6831558 None:intergenic
CAAGAGCAGCATAGGATGAA+GGG 0.580724 4.2:-6831356 None:intergenic
TTGTGATAAAACTGTTCACT+TGG 0.580761 4.2:+6829992 MS.gene72173:CDS
GCCTCCAAGAAACCCCAGAC+GGG 0.596184 4.2:+6830599 MS.gene72173:CDS
TGATCCTGCTGTTACACAAG+AGG 0.603788 4.2:+6831554 MS.gene72173:CDS
AGACACAGAGACAGAGACAA+AGG 0.606794 4.2:+6831524 MS.gene72173:CDS
CGCCTCCAAGAAACCCCAGA+CGG 0.608247 4.2:+6830598 MS.gene72173:CDS
AGTAACTATCAATCAATCGA+AGG 0.610154 4.2:+6830527 MS.gene72173:CDS
TTCAGATGTAATCAATGCAA+TGG 0.611041 4.2:+6830059 MS.gene72173:CDS
CGTATGTATATACCGCGAGA+AGG 0.635292 4.2:-6830082 None:intergenic
TTATGACAGTTGACAGTGGA+AGG 0.635630 4.2:+6831279 MS.gene72173:intron
AGGATCTTATTACCCGTCTG+GGG 0.646532 4.2:-6830611 None:intergenic
GAAAGCAGAAAGTTTGCTTG+GGG 0.651424 4.2:-6830884 None:intergenic
TGGAACCCAACAGAAATGCA+AGG 0.666590 4.2:+6829968 MS.gene72173:CDS
TGCTTTAGGTGCACACATGG+AGG 0.676247 4.2:+6831321 MS.gene72173:CDS
CTGTTTATGACAGTTGACAG+TGG 0.680767 4.2:+6831275 MS.gene72173:intron
TTAAACAACTGAGAAAAGTG+GGG 0.688189 4.2:-6831399 None:intergenic
TTGGGTTCCAGTGAAAGACA+TGG 0.695332 4.2:-6829955 None:intergenic
TCAATGCAATGGCCTTCTCG+CGG 0.702963 4.2:+6830070 MS.gene72173:CDS
TGCACCTAAAGCATGACTTG+TGG 0.763367 4.2:-6831311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCCTATTATTATAATT+TGG - chr4.2:6830664-6830683 None:intergenic 10.0%
!! ATATATATATATATCAAAGA+TGG + chr4.2:6830171-6830190 MS.gene72173:intron 10.0%
!! TATATATATATATCAAAGAT+GGG + chr4.2:6830172-6830191 MS.gene72173:intron 10.0%
!!! TTATCTATGTTTAATTTTTT+TGG + chr4.2:6830310-6830329 MS.gene72173:intron 10.0%
!! AAGTATATAAAAAAAAGGAA+AGG - chr4.2:6830634-6830653 None:intergenic 15.0%
!! ATATATATGATCAGAAAAAA+GGG - chr4.2:6831060-6831079 None:intergenic 15.0%
!! ATCACTAATAATAACATAAA+TGG + chr4.2:6830484-6830503 MS.gene72173:intron 15.0%
!! CTATACCAAATTATAATAAT+AGG + chr4.2:6830656-6830675 MS.gene72173:intron 15.0%
!! TAGACAAGTATATAAAAAAA+AGG - chr4.2:6830639-6830658 None:intergenic 15.0%
!! TATATATATGATCAGAAAAA+AGG - chr4.2:6831061-6831080 None:intergenic 15.0%
!!! ATTTTTACAAGTAAAAGTTT+AGG - chr4.2:6830443-6830462 None:intergenic 15.0%
!! ATATATATAGAACATAAACG+AGG - chr4.2:6830157-6830176 None:intergenic 20.0%
!! TATATATAGAACATAAACGA+GGG - chr4.2:6830156-6830175 None:intergenic 20.0%
!! TTATGAAACACAAAATGATA+GGG + chr4.2:6830225-6830244 MS.gene72173:intron 20.0%
!! TTTATGAAACACAAAATGAT+AGG + chr4.2:6830224-6830243 MS.gene72173:intron 20.0%
!!! ATATAGTTCTATTGTTTTAG+TGG - chr4.2:6831004-6831023 None:intergenic 20.0%
!!! ATTGGAAAAGGTATATATTT+GGG + chr4.2:6830957-6830976 MS.gene72173:intron 20.0%
! ACCATATAGCAAAAACAAAT+TGG - chr4.2:6830133-6830152 None:intergenic 25.0%
! ACTATATAATCAAACAATGC+TGG + chr4.2:6831017-6831036 MS.gene72173:intron 25.0%
!! CAAATATATACCTTTTCCAA+TGG - chr4.2:6830958-6830977 None:intergenic 25.0%
!! CATTGGAAAAGGTATATATT+TGG + chr4.2:6830956-6830975 MS.gene72173:intron 25.0%
!!! ACCAATTTGTTTTTGCTATA+TGG + chr4.2:6830129-6830148 MS.gene72173:intron 25.0%
!!! ACGTATCTTAATTTGTTTTC+TGG + chr4.2:6830102-6830121 MS.gene72173:intron 25.0%
AACTGTATATCCATTGGAAA+AGG + chr4.2:6830945-6830964 MS.gene72173:CDS 30.0%
AGTAACTATCAATCAATCGA+AGG + chr4.2:6830527-6830546 MS.gene72173:CDS 30.0%
CAAGAAAACTGTATATCCAT+TGG + chr4.2:6830939-6830958 MS.gene72173:CDS 30.0%
CTTAAACAACTGAGAAAAGT+GGG - chr4.2:6831403-6831422 None:intergenic 30.0%
GTATAAAATGTCACAATGTC+TGG + chr4.2:6831202-6831221 MS.gene72173:intron 30.0%
TATATATACTCCAACAATGC+TGG + chr4.2:6831073-6831092 MS.gene72173:intron 30.0%
TGCATAATCATAAACGACAT+GGG + chr4.2:6830372-6830391 MS.gene72173:intron 30.0%
TTAAACAACTGAGAAAAGTG+GGG - chr4.2:6831402-6831421 None:intergenic 30.0%
TTCAGATGTAATCAATGCAA+TGG + chr4.2:6830059-6830078 MS.gene72173:CDS 30.0%
TTGCATAATCATAAACGACA+TGG + chr4.2:6830371-6830390 MS.gene72173:intron 30.0%
TTGTGATAAAACTGTTCACT+TGG + chr4.2:6829992-6830011 MS.gene72173:CDS 30.0%
TTTCATATGTTTACAGCCTA+GGG + chr4.2:6830861-6830880 MS.gene72173:intron 30.0%
! GACAGTATAAGTACTTTTCT+AGG + chr4.2:6830400-6830419 MS.gene72173:intron 30.0%
! TTTAGGTGAGTTGAATATGT+TGG - chr4.2:6830426-6830445 None:intergenic 30.0%
! TTTCATGATTTTCAAACAGC+AGG + chr4.2:6830727-6830746 MS.gene72173:intron 30.0%
!!! AGGAATTTATGTGCTGTTTT+TGG + chr4.2:6830676-6830695 MS.gene72173:intron 30.0%
!!! GAATTTATGTGCTGTTTTTG+GGG + chr4.2:6830678-6830697 MS.gene72173:intron 30.0%
!!! GGAATTTATGTGCTGTTTTT+GGG + chr4.2:6830677-6830696 MS.gene72173:intron 30.0%
AAACTTTCTGCTTTCTTCTC+AGG + chr4.2:6830892-6830911 MS.gene72173:CDS 35.0%
AACTTTCTGCTTTCTTCTCA+GGG + chr4.2:6830893-6830912 MS.gene72173:CDS 35.0%
ATGGGTCAAATTGCATCTAA+AGG + chr4.2:6830190-6830209 MS.gene72173:intron 35.0%
CCTTAAACAACTGAGAAAAG+TGG - chr4.2:6831404-6831423 None:intergenic 35.0%
CTTTCATATGTTTACAGCCT+AGG + chr4.2:6830860-6830879 MS.gene72173:intron 35.0%
GGAAACGGGTGTATTATATA+CGG - chr4.2:6831134-6831153 None:intergenic 35.0%
GTATATAATACACCCGTTTC+CGG + chr4.2:6831133-6831152 MS.gene72173:intron 35.0%
TAATCTAAACACACCGGAAA+CGG - chr4.2:6831149-6831168 None:intergenic 35.0%
TACTCTCGATGATCTTAAGA+AGG + chr4.2:6830345-6830364 MS.gene72173:intron 35.0%
TCCTCATAATCTAAACACAC+CGG - chr4.2:6831155-6831174 None:intergenic 35.0%
TTTACCACAAGTCATGCTTT+AGG + chr4.2:6831307-6831326 MS.gene72173:CDS 35.0%
! AAGAAAGCAGAAAGTTTGCT+TGG - chr4.2:6830889-6830908 None:intergenic 35.0%
! AGAAAGCAGAAAGTTTGCTT+GGG - chr4.2:6830888-6830907 None:intergenic 35.0%
! TCTCAGTTGTTTAAGGCAAA+AGG + chr4.2:6831408-6831427 MS.gene72173:CDS 35.0%
!! CCACTTTTCTCAGTTGTTTA+AGG + chr4.2:6831401-6831420 MS.gene72173:CDS 35.0%
AAAGGATCTTATTACCCGTC+TGG - chr4.2:6830616-6830635 None:intergenic 40.0%
AAGGATCTTATTACCCGTCT+GGG - chr4.2:6830615-6830634 None:intergenic 40.0%
AATCTAAACACACCGGAAAC+GGG - chr4.2:6831148-6831167 None:intergenic 40.0%
ACAAGCCTTGCATTTCTGTT+GGG - chr4.2:6829976-6829995 None:intergenic 40.0%
AGAATACACACCAGTTCACT+AGG - chr4.2:6830757-6830776 None:intergenic 40.0%
CTGTTTATGACAGTTGACAG+TGG + chr4.2:6831275-6831294 MS.gene72173:intron 40.0%
GTTGATACGTTATCTGCTGA+TGG + chr4.2:6830014-6830033 MS.gene72173:CDS 40.0%
TACTCCAACAATGCTGGATT+TGG + chr4.2:6831079-6831098 MS.gene72173:intron 40.0%
TCCGGTGTGTTTAGATTATG+AGG + chr4.2:6831151-6831170 MS.gene72173:intron 40.0%
TTATGACAGTTGACAGTGGA+AGG + chr4.2:6831279-6831298 MS.gene72173:intron 40.0%
! GAAAGCAGAAAGTTTGCTTG+GGG - chr4.2:6830887-6830906 None:intergenic 40.0%
AAAAGGGCCCACTGAAATCA+TGG - chr4.2:6831044-6831063 None:intergenic 45.0%
AGACACAGAGACAGAGACAA+AGG + chr4.2:6831524-6831543 MS.gene72173:CDS 45.0%
AGGATCTTATTACCCGTCTG+GGG - chr4.2:6830614-6830633 None:intergenic 45.0%
ATGCTGGACCATGATTTCAG+TGG + chr4.2:6831033-6831052 MS.gene72173:intron 45.0%
CAAGAGCAGCATAGGATGAA+GGG - chr4.2:6831359-6831378 None:intergenic 45.0%
CACAAGCCTTGCATTTCTGT+TGG - chr4.2:6829977-6829996 None:intergenic 45.0%
CGTATGTATATACCGCGAGA+AGG - chr4.2:6830085-6830104 None:intergenic 45.0%
GAATCCATCAAGAGCAGCAT+AGG - chr4.2:6831367-6831386 None:intergenic 45.0%
GTGTCCAAATCCAGCATTGT+TGG - chr4.2:6831086-6831105 None:intergenic 45.0%
TACACCTCTTGTGTAACAGC+AGG - chr4.2:6831561-6831580 None:intergenic 45.0%
TCAAGAGCAGCATAGGATGA+AGG - chr4.2:6831360-6831379 None:intergenic 45.0%
TCATCCTATGCTGCTCTTGA+TGG + chr4.2:6831360-6831379 MS.gene72173:CDS 45.0%
TGATCCTGCTGTTACACAAG+AGG + chr4.2:6831554-6831573 MS.gene72173:CDS 45.0%
TGCACCTAAAGCATGACTTG+TGG - chr4.2:6831314-6831333 None:intergenic 45.0%
TGCTGGACCATGATTTCAGT+GGG + chr4.2:6831034-6831053 MS.gene72173:intron 45.0%
TGGAACCCAACAGAAATGCA+AGG + chr4.2:6829968-6829987 MS.gene72173:CDS 45.0%
! TCATGCTTTAGGTGCACACA+TGG + chr4.2:6831318-6831337 MS.gene72173:CDS 45.0%
AGCAGGAAAGCCTAGTGAAC+TGG + chr4.2:6830744-6830763 MS.gene72173:intron 50.0%
TCAATGCAATGGCCTTCTCG+CGG + chr4.2:6830070-6830089 MS.gene72173:CDS 50.0%
TCTGTCTCTGTGTCTGATGC+TGG - chr4.2:6831519-6831538 None:intergenic 50.0%
! ATTACCCGTCTGGGGTTTCT+TGG - chr4.2:6830606-6830625 None:intergenic 50.0%
! TCACGCTGAGCAGCTTTTCA+AGG + chr4.2:6830568-6830587 MS.gene72173:CDS 50.0%
! TGCTTTAGGTGCACACATGG+AGG + chr4.2:6831321-6831340 MS.gene72173:CDS 50.0%
! GAAAGTTTGCTTGGGGCCCT+AGG - chr4.2:6830880-6830899 None:intergenic 55.0%
CGCCTCCAAGAAACCCCAGA+CGG + chr4.2:6830598-6830617 MS.gene72173:CDS 60.0%
CTGGGGTTTCTTGGAGGCGA+AGG - chr4.2:6830597-6830616 None:intergenic 60.0%
GCCTCCAAGAAACCCCAGAC+GGG + chr4.2:6830599-6830618 MS.gene72173:CDS 60.0%
! ACCCGTCTGGGGTTTCTTGG+AGG - chr4.2:6830603-6830622 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 6829957 6831581 6829957 ID=MS.gene72173
chr4.2 mRNA 6829957 6831581 6829957 ID=MS.gene72173.t1;Parent=MS.gene72173
chr4.2 exon 6829957 6830091 6829957 ID=MS.gene72173.t1.exon1;Parent=MS.gene72173.t1
chr4.2 CDS 6829957 6830091 6829957 ID=cds.MS.gene72173.t1;Parent=MS.gene72173.t1
chr4.2 exon 6830524 6830620 6830524 ID=MS.gene72173.t1.exon2;Parent=MS.gene72173.t1
chr4.2 CDS 6830524 6830620 6830524 ID=cds.MS.gene72173.t1;Parent=MS.gene72173.t1
chr4.2 exon 6830746 6830765 6830746 ID=MS.gene72173.t1.exon3;Parent=MS.gene72173.t1
chr4.2 CDS 6830746 6830765 6830746 ID=cds.MS.gene72173.t1;Parent=MS.gene72173.t1
chr4.2 exon 6830877 6830966 6830877 ID=MS.gene72173.t1.exon4;Parent=MS.gene72173.t1
chr4.2 CDS 6830877 6830966 6830877 ID=cds.MS.gene72173.t1;Parent=MS.gene72173.t1
chr4.2 exon 6831288 6831581 6831288 ID=MS.gene72173.t1.exon5;Parent=MS.gene72173.t1
chr4.2 CDS 6831288 6831581 6831288 ID=cds.MS.gene72173.t1;Parent=MS.gene72173.t1
Gene Sequence

>MS.gene72173

ATGTCTTTCACTGGAACCCAACAGAAATGCAAGGCTTGTGATAAAACTGTTCACTTGGTTGATACGTTATCTGCTGATGGTAATGTTTTTCACAAGAACTGCTTCAGATGTAATCAATGCAATGGCCTTCTCGCGGTATATACATACGTATCTTAATTTGTTTTCTGGTTTTACCAATTTGTTTTTGCTATATGGTCCCTCGTTTATGTTCTATATATATATATATATCAAAGATGGGTCAAATTGCATCTAAAGGCAGTTTTTTTTTTTATGAAACACAAAATGATAGGGACTACACTATGTAAACACAAATATAAGCAAAAAATAATCAATTGCATGTCGATAAAAAATAGTTATCTATGTTTAATTTTTTTGGTATATAAATTTATACTCTCGATGATCTTAAGAAGGAGCTTGCATAATCATAAACGACATGGGATTGTGACAGTATAAGTACTTTTCTAGGCCAACATATTCAACTCACCTAAACTTTTACTTGTAAAAATAAATAAATCGAAATACAAATAATCACTAATAATAACATAAATGGTTTAATTTTCATTGCAGATGAGTAACTATCAATCAATCGAAGGAGTTTTATACTGCAAGACTCACGCTGAGCAGCTTTTCAAGGATTCCTTCGCCTCCAAGAAACCCCAGACGGGTAATAAGATCCTTTCCTTTTTTTTATATACTTGTCTATACCAAATTATAATAATAGGAATTTATGTGCTGTTTTTGGGGCTAAATAATCACTTAATCTTAATTTGTTTCATGATTTTCAAACAGCAGGAAAGCCTAGTGAACTGGTGTGTATTCTATTCACTATTGATACAACACTCATTTACTTTTTTCTAATATTGTTCTTCTAGTATAACTTCAATTTACAATATAATAAAATTTCTTTCATATGTTTACAGCCTAGGGCCCCAAGCAAACTTTCTGCTTTCTTCTCAGGGACTCAAGACAAATGTTCATCATGCAAGAAAACTGTATATCCATTGGAAAAGGTATATATTTGGGTTAATTTCAGATATTATTGTACCACTAAAACAATAGAACTATATAATCAAACAATGCTGGACCATGATTTCAGTGGGCCCTTTTTTCTGATCATATATATACTCCAACAATGCTGGATTTGGACACAAAAATTCGATACATTATACTGATACCGTATATAATACACCCGTTTCCGGTGTGTTTAGATTATGAGGATTTTTTTTTTATATAAAATCGAAAAGCGTATAAAATGTCACAATGTCTGGAGTCAAATTCTGATGTCTAAAAAACATTTCTGTACTTTAATTGTGAAATTCTGTTTATGACAGTTGACAGTGGAAGGTGAGTTTTACCACAAGTCATGCTTTAGGTGCACACATGGAGGATGTTTTCTCAGCCCTTCATCCTATGCTGCTCTTGATGGATTCATCTACTGCAAACCCCACTTTTCTCAGTTGTTTAAGGCAAAAGGTAGCTATAGTTATCTCTCTAAACAAGCTTCAATAAAGAAAAATGAAGAAATGCAACAAGCTGCAGCAGAGAAAAATGAATCAGCACCAGCATCAGACACAGAGACAGAGACAAAGGAAGAACATGATCCTGCTGTTACACAAGAGGTGTAG
Protein sequence

>MS.gene72173.t1

MSFTGTQQKCKACDKTVHLVDTLSADGNVFHKNCFRCNQCNGLLAMSNYQSIEGVLYCKTHAEQLFKDSFASKKPQTAGKPSELPRAPSKLSAFFSGTQDKCSSCKKTVYPLEKLTVEGEFYHKSCFRCTHGGCFLSPSSYAALDGFIYCKPHFSQLFKAKGSYSYLSKQASIKKNEEMQQAAAEKNESAPASDTETETKEEHDPAVTQEV