AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72309

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72309.t1 MTR_1g060700 94.569 313 13 3 1 312 178 487 0.0 599
MS.gene72309.t1 MTR_2g067000 81.677 322 25 10 1 312 1 298 5.22e-164 458
MS.gene72309.t1 MTR_8g093500 58.491 53 22 0 182 234 125 177 2.82e-13 69.3
MS.gene72309.t1 MTR_3g101810 58.491 53 22 0 182 234 182 234 1.73e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72309.t1 AT1G30670 41.520 171 97 2 135 305 95 262 3.00e-29 113
MS.gene72309.t1 AT2G34820 42.254 213 115 4 97 305 81 289 2.84e-28 110
MS.gene72309.t1 AT3G21330 60.377 53 21 0 182 234 280 332 2.13e-13 70.5
MS.gene72309.t1 AT5G01305 50.000 58 29 0 182 239 48 105 1.36e-12 65.5
MS.gene72309.t1 AT4G00120 52.830 53 25 0 182 234 123 175 6.58e-12 63.9

Find 54 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGAATGCTTCAACTTA+TGG 0.195386 1.4:-49722452 MS.gene72309:CDS
ATCTCTGCTGTGTTCATCTT+TGG 0.238213 1.4:+49722517 None:intergenic
TTGGTGAAATCTTCCTCATT+TGG 0.290806 1.4:+49722838 None:intergenic
ATCAAAACCTACCATTCTTA+AGG 0.300143 1.4:-49721410 MS.gene72309:CDS
TCAAATAGTTAGAGGGAAAA+TGG 0.318116 1.4:+49723013 None:intergenic
AAATAAAACTATTCTTGCTT+TGG 0.325467 1.4:+49721349 None:intergenic
TGGTGAAATCTTCCTCATTT+GGG 0.332786 1.4:+49722839 None:intergenic
GAAGCAAAAGGGTTAGGGTT+AGG 0.345524 1.4:+49722703 None:intergenic
AAGCAAAAGGGTTAGGGTTA+GGG 0.345934 1.4:+49722704 None:intergenic
TCTCCTCGTAGCATAGCTTT+TGG 0.368098 1.4:+49722794 None:intergenic
AGTTGATCTTGACAAGGGTT+AGG 0.369040 1.4:+49722922 None:intergenic
TCAAAGGAAGCAAAAGGGTT+AGG 0.369513 1.4:+49722697 None:intergenic
AGTAGTGATTTCTAAGTTGT+TGG 0.372241 1.4:+49723077 None:intergenic
GTAGTTTGAATAGGTTGATA+AGG 0.378786 1.4:+49723099 None:intergenic
TACAGTTTCTCTTGAACAAA+TGG 0.383908 1.4:+49721499 None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG 0.388228 1.4:-49721391 MS.gene72309:CDS
AATGTGGTGACAAACTCTTT+TGG 0.390869 1.4:+49721454 None:intergenic
GAGCTTGGGAAGTTGGTTCC+TGG 0.392352 1.4:-49722544 MS.gene72309:CDS
GACACAAGAGCTTGGGAAGT+TGG 0.397999 1.4:-49722551 MS.gene72309:CDS
AAGTTGTTGGTAGTTTGAAT+AGG 0.402079 1.4:+49723090 None:intergenic
TTGATTATTTCAGGAAGAAA+AGG 0.406675 1.4:-49721563 MS.gene72309:intron
TAGTTTGAATAGGTTGATAA+GGG 0.429054 1.4:+49723100 None:intergenic
AGTTTCATTCAAATAGTTAG+AGG 0.435549 1.4:+49723005 None:intergenic
GTTGATCTTGACAAGGGTTA+GGG 0.453473 1.4:+49722923 None:intergenic
TCAAAACCTACCATTCTTAA+GGG 0.456430 1.4:-49721409 MS.gene72309:CDS
CAAAGGAAGCAAAAGGGTTA+GGG 0.461232 1.4:+49722698 None:intergenic
AGAGTATCGCGGCGAGAGAA+AGG 0.466876 1.4:-49722594 MS.gene72309:CDS
TCTACCTCAAAGGAAGCAAA+AGG 0.468900 1.4:+49722691 None:intergenic
TTGTGTAGATCTTCACTTGG+AGG 0.473071 1.4:+49721535 None:intergenic
GTTCAATGTGAGAAGAAAGT+TGG 0.488552 1.4:-49722637 MS.gene72309:CDS
TGTAGATCTTCACTTGGAGG+AGG 0.494574 1.4:+49721538 None:intergenic
GTGTTCATCTTTGGTCCACC+AGG 0.499817 1.4:+49722526 None:intergenic
ACTGAGAAGACACAAGAGCT+TGG 0.499993 1.4:-49722559 MS.gene72309:CDS
CTGAGAAGACACAAGAGCTT+GGG 0.500383 1.4:-49722558 MS.gene72309:CDS
CTGTTTCAGACCCTTAAGAA+TGG 0.516519 1.4:+49721399 None:intergenic
AGGGTCTGAAACAGCTTGCT+GGG 0.522554 1.4:-49721390 MS.gene72309:CDS
GTTTCATTCAAATAGTTAGA+GGG 0.549802 1.4:+49723006 None:intergenic
CTTGGGAAGTTGGTTCCTGG+TGG 0.551828 1.4:-49722541 MS.gene72309:CDS
GTGAGAAGAAAGTTGGTGAA+AGG 0.566992 1.4:-49722630 MS.gene72309:CDS
AAGTTTCTTCAAGCTCAAGT+TGG 0.567049 1.4:-49722469 MS.gene72309:CDS
AGCAAAGGGTTCTACCTCAA+AGG 0.572532 1.4:+49722681 None:intergenic
AGTTTGTGTAGATCTTCACT+TGG 0.582386 1.4:+49721532 None:intergenic
TTCAGACCCTTAAGAATGGT+AGG 0.583734 1.4:+49721403 None:intergenic
GAGTTCAAAAGCTCTTGTTG+TGG 0.586358 1.4:+49722760 None:intergenic
TCGCGGCGAGAGAAAGGAGA+AGG 0.587504 1.4:-49722588 MS.gene72309:CDS
ACTTATGGAAACATTTAACA+AGG 0.595324 1.4:-49722437 MS.gene72309:intron
CTACCTCAAAGGAAGCAAAA+GGG 0.620756 1.4:+49722692 None:intergenic
AATAGTTAGAGGGAAAATGG+TGG 0.625963 1.4:+49723016 None:intergenic
ACAAGTAGTTGATCTTGACA+AGG 0.633389 1.4:+49722916 None:intergenic
CAAGTAGTTGATCTTGACAA+GGG 0.656926 1.4:+49722917 None:intergenic
ATCATTATGATTGCTCAATG+TGG 0.666694 1.4:+49721438 None:intergenic
ATGCTACGAGGAGAAAAGAG+AGG 0.681365 1.4:-49722785 MS.gene72309:CDS
TATCTCGTCGCAGAGTATCG+CGG 0.686396 1.4:-49722605 MS.gene72309:CDS
ACGCCAAAAGCTATGCTACG+AGG 0.715032 1.4:-49722797 MS.gene72309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTGTAAATATCTAAA+TGG - chr1.4:49722434-49722453 MS.gene72309:intron 15.0%
!! CATAATAACACTACAATAAA+TGG + chr1.4:49722409-49722428 None:intergenic 20.0%
!! CGTTTGATAAAAAAATATAG+AGG - chr1.4:49722378-49722397 MS.gene72309:intron 20.0%
!! GGAATTGTTATAAAAATTGA+CGG - chr1.4:49722590-49722609 MS.gene72309:CDS 20.0%
!! TTTACTCCATGAAAATATTT+AGG - chr1.4:49722569-49722588 MS.gene72309:CDS 20.0%
!! TTTCATTTGAGTAAAAACAA+GGG + chr1.4:49721548-49721567 None:intergenic 20.0%
!!! ACAATTCCTAAATATTTTCA+TGG + chr1.4:49722578-49722597 None:intergenic 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1.4:49722923-49722942 MS.gene72309:CDS 20.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1.4:49721763-49721782 None:intergenic 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1.4:49722522-49722541 None:intergenic 25.0%
! ACTTATGGAAACATTTAACA+AGG - chr1.4:49722058-49722077 MS.gene72309:intron 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1.4:49722210-49722229 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1.4:49721493-49721512 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1.4:49721492-49721511 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1.4:49721549-49721568 None:intergenic 25.0%
! TAACTGTTTAACAAGGAATT+TGG - chr1.4:49722784-49722803 MS.gene72309:CDS 25.0%
! TGTTAGGTTTAAGAAAATGT+TGG - chr1.4:49722620-49722639 MS.gene72309:CDS 25.0%
! TTCATTTGAGTAAAAACAAG+GGG + chr1.4:49721547-49721566 None:intergenic 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1.4:49722932-49722951 MS.gene72309:CDS 25.0%
! TTGTTATAAAAATTGACGGA+TGG - chr1.4:49722594-49722613 MS.gene72309:CDS 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1.4:49722310-49722329 MS.gene72309:intron 25.0%
!! TATTTTCATGGAGTAAAAGT+TGG + chr1.4:49722566-49722585 None:intergenic 25.0%
!! TTAAACTGTTTTGTGTACTT+AGG - chr1.4:49722170-49722189 MS.gene72309:intron 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1.4:49721609-49721628 MS.gene72309:intron 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1.4:49721610-49721629 None:intergenic 25.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1.4:49721398-49721417 None:intergenic 25.0%
ACTCAAATGATTACAAGCAA+GGG + chr1.4:49722352-49722371 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1.4:49721421-49721440 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1.4:49723085-49723104 MS.gene72309:CDS 30.0%
ATCATTATGATTGCTCAATG+TGG + chr1.4:49723060-49723079 None:intergenic 30.0%
TAATGCTGTTGTTGTAGATT+TGG - chr1.4:49722461-49722480 MS.gene72309:CDS 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1.4:49722999-49723018 None:intergenic 30.0%
TACTCAAATGATTACAAGCA+AGG + chr1.4:49722353-49722372 None:intergenic 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1.4:49723086-49723105 MS.gene72309:CDS 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1.4:49721485-49721504 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1.4:49721644-49721663 MS.gene72309:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1.4:49721679-49721698 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1.4:49721408-49721427 None:intergenic 30.0%
!! CAATAAACGAGAGAGTATTT+AGG - chr1.4:49722728-49722747 MS.gene72309:CDS 30.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1.4:49721399-49721418 None:intergenic 30.0%
!!! AACAATTTTTGAAGCAGCAA+AGG + chr1.4:49721832-49721851 None:intergenic 30.0%
!!! ACAATTTTTGAAGCAGCAAA+GGG + chr1.4:49721831-49721850 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1.4:49721752-49721771 MS.gene72309:intron 30.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1.4:49722026-49722045 MS.gene72309:intron 35.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1.4:49721482-49721501 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1.4:49721582-49721601 None:intergenic 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1.4:49722698-49722717 MS.gene72309:CDS 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1.4:49722043-49722062 MS.gene72309:intron 35.0%
AGTTTGTGTAGATCTTCACT+TGG + chr1.4:49722966-49722985 None:intergenic 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1.4:49722513-49722532 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1.4:49721581-49721600 None:intergenic 35.0%
CAAGTGTATATCAAATGAGC+TGG - chr1.4:49722756-49722775 MS.gene72309:CDS 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1.4:49722704-49722723 MS.gene72309:CDS 35.0%
GCAATGAACAAACAAACTCT+AGG + chr1.4:49722305-49722324 None:intergenic 35.0%
GGCTATGTAACTGTTTAACA+AGG - chr1.4:49722777-49722796 MS.gene72309:CDS 35.0%
GTTCAATGTGAGAAGAAAGT+TGG - chr1.4:49721858-49721877 MS.gene72309:intron 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1.4:49721659-49721678 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1.4:49721660-49721679 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1.4:49723044-49723063 None:intergenic 35.0%
! AATTGACGGATGGAATTGTT+AGG - chr1.4:49722604-49722623 MS.gene72309:CDS 35.0%
AAGCAAAAGGGTTAGGGTTA+GGG + chr1.4:49721794-49721813 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1.4:49721576-49721595 None:intergenic 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1.4:49721981-49722000 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1.4:49721800-49721819 None:intergenic 40.0%
CTACCTCAAAGGAAGCAAAA+GGG + chr1.4:49721806-49721825 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1.4:49723099-49723118 None:intergenic 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1.4:49721738-49721757 None:intergenic 40.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1.4:49721575-49721594 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1.4:49721801-49721820 None:intergenic 40.0%
TCTACCTCAAAGGAAGCAAA+AGG + chr1.4:49721807-49721826 None:intergenic 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1.4:49723095-49723114 None:intergenic 40.0%
TTGTGTAGATCTTCACTTGG+AGG + chr1.4:49722963-49722982 None:intergenic 40.0%
! GAGAGTGTGTGTTAAGGAAA+CGG - chr1.4:49722684-49722703 MS.gene72309:CDS 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1.4:49721800-49721819 MS.gene72309:intron 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1.4:49721696-49721715 None:intergenic 40.0%
!! GTGAGAAGAAAGTTGGTGAA+AGG - chr1.4:49721865-49721884 MS.gene72309:intron 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1.4:49721686-49721705 None:intergenic 40.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1.4:49721936-49721955 MS.gene72309:intron 45.0%
AGCAAAGGGTTCTACCTCAA+AGG + chr1.4:49721817-49721836 None:intergenic 45.0%
ATGCTACGAGGAGAAAAGAG+AGG - chr1.4:49721710-49721729 MS.gene72309:intron 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1.4:49721937-49721956 MS.gene72309:intron 45.0%
GAAGCAAAAGGGTTAGGGTT+AGG + chr1.4:49721795-49721814 None:intergenic 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1.4:49722960-49722979 None:intergenic 45.0%
! GAGAGAGAGAGTGTGTGTTA+AGG - chr1.4:49722678-49722697 MS.gene72309:CDS 45.0%
! TCTCCTCGTAGCATAGCTTT+TGG + chr1.4:49721704-49721723 None:intergenic 45.0%
ACGCCAAAAGCTATGCTACG+AGG - chr1.4:49721698-49721717 MS.gene72309:intron 50.0%
GACACAAGAGCTTGGGAAGT+TGG - chr1.4:49721944-49721963 MS.gene72309:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1.4:49721972-49721991 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1.4:49723104-49723123 MS.gene72309:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1.4:49723105-49723124 MS.gene72309:CDS 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1.4:49721890-49721909 MS.gene72309:intron 50.0%
! CTTGGGAAGTTGGTTCCTGG+TGG - chr1.4:49721954-49721973 MS.gene72309:intron 55.0%
! GAGCTTGGGAAGTTGGTTCC+TGG - chr1.4:49721951-49721970 MS.gene72309:intron 55.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1.4:49721901-49721920 MS.gene72309:intron 55.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1.4:49721907-49721926 MS.gene72309:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 49721357 49723160 49721357 ID=MS.gene72309
chr1.4 mRNA 49721357 49723160 49721357 ID=MS.gene72309.t1;Parent=MS.gene72309
chr1.4 exon 49722438 49723160 49722438 ID=MS.gene72309.t1.exon1;Parent=MS.gene72309.t1
chr1.4 CDS 49722438 49723160 49722438 ID=cds.MS.gene72309.t1;Parent=MS.gene72309.t1
chr1.4 exon 49721357 49721572 49721357 ID=MS.gene72309.t1.exon2;Parent=MS.gene72309.t1
chr1.4 CDS 49721357 49721572 49721357 ID=cds.MS.gene72309.t1;Parent=MS.gene72309.t1
Gene Sequence

>MS.gene72309

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCTTTTAGATGAAAGCTTCATTTTTCAAACCCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGAGGAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTAACCCTTTTGCTTCCTTTGAGGTAGAACCCTTTGCTGCTTCAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGGTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAGTTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAACAAGGTAACTGTTTTTGTTTTTATTGTTGCAATGTTCTTATATTATTAGAGCTCATAATTGAAGTTAATTACTTGTTTTCACTTGAGTTTCAATTTAAACTGTTTTGTGTACTTAGGTCTTATTTAATACTCCAAGTGAATTTGATGTTTTTCTTTTGAATGATGTTTTTTGCTTTTTATATGTTATGCTATGCTACTTGATTTAGTTTTTGAGTTTATAATAATACCTAGAGTTTGTTTGTTCATTGCATATTTGGTTACTTTGTGTGAAGACCCTTGCTTGTAATCATTTGAGTACATGTCGTTTGATAAAAAAATATAGAGGATATGCCATTTATTGTAGTGTTATTATGTCATGTATTGTTGTAAATATCTAAATGGATATTAATGCTGTTGTTGTAGATTTGGTTAGTTTTATCATTTATGTATGTAATCCATGACTACCTATTGTCTCAATTTTGTCTTTTTTATATTGATTTTTTTCATACCAACTTTTACTCCATGAAAATATTTAGGAATTGTTATAAAAATTGACGGATGGAATTGTTAGGTTTAAGAAAATGTTGGTTACAATGTATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGTGTGTTAAGGAAACGGTTAATAAGAAGAGGAAATGGTCAATAAACGAGAGAGTATTTAGGAACAGCAAGTGTATATCAAATGAGCTGGCTATGTAACTGTTTAACAAGGAATTTGGCAATTTTGCAATATTTTTTTAAATTTAAATATTCACTATTTAGTGCAACATTTTTGTAAAAGCTAAATTTCTAGTGTAACTTATATAATAAAAAGAATTCACGTATATATCTTAGATTTTGCTAATTGATTATTTCAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACACAAACTTCTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATGATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG
Protein sequence

>MS.gene72309.t1

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSLLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYEEKREEPQQELLNSTNFFVDEFMTNPNPNPFASFEVEPFAASKIVDVVQCEKKVGERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFNKEEKEPPPSEDLHKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNDVQSKPTILKGLKQLAGTEIEKKPKQE