AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72494

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72494.t1 MTR_1g047060 51.064 94 39 2 109 200 1 89 5.97e-23 88.6
MS.gene72494.t1 MTR_7g105170 29.885 174 98 7 1 152 1 172 4.43e-17 77.4
MS.gene72494.t1 MTR_6g477900 32.386 176 89 8 4 165 6 165 2.36e-16 75.1
MS.gene72494.t1 MTR_1g096430 30.769 156 92 5 4 148 7 157 7.82e-16 75.1
MS.gene72494.t1 MTR_5g012080 31.210 157 86 6 4 145 7 156 2.24e-15 73.9
MS.gene72494.t1 MTR_2g062730 30.769 156 87 5 4 145 10 158 4.42e-15 72.8
MS.gene72494.t1 MTR_8g023840 32.903 155 82 8 4 145 11 156 4.52e-15 73.2
MS.gene72494.t1 MTR_2g086880 31.081 148 83 6 6 144 20 157 4.68e-15 73.6
MS.gene72494.t1 MTR_2g086880 31.081 148 83 6 6 144 20 157 5.27e-15 73.2
MS.gene72494.t1 MTR_7g100990 34.161 161 77 9 4 144 11 162 6.87e-15 72.4
MS.gene72494.t1 MTR_1g008740 33.758 157 85 7 2 145 25 175 7.30e-15 72.4
MS.gene72494.t1 MTR_8g023900 33.333 156 80 8 4 144 12 158 7.88e-15 72.8
MS.gene72494.t1 MTR_8g023900 31.333 150 83 7 7 144 196 337 1.44e-11 63.2
MS.gene72494.t1 MTR_1g008740 33.758 157 85 7 2 145 11 161 1.01e-14 72.0
MS.gene72494.t1 MTR_4g101680 32.051 156 84 6 4 145 7 154 1.83e-14 71.2
MS.gene72494.t1 MTR_3g096140 32.704 159 84 7 2 145 5 155 2.89e-14 70.1
MS.gene72494.t1 MTR_8g023860 30.573 157 89 7 1 145 9 157 5.47e-14 70.1
MS.gene72494.t1 MTR_3g096140 32.704 159 84 7 2 145 5 155 6.53e-14 69.7
MS.gene72494.t1 MTR_1g069805 33.766 154 81 9 4 145 10 154 1.44e-13 68.2
MS.gene72494.t1 MTR_7g097090 34.416 154 80 9 4 145 10 154 6.32e-13 66.2
MS.gene72494.t1 MTR_6g011860 29.268 164 87 6 4 145 15 171 6.51e-13 67.0
MS.gene72494.t1 MTR_8g093790 29.240 171 100 6 4 171 10 162 1.12e-12 64.3
MS.gene72494.t1 MTR_8g094580 28.750 160 88 6 4 146 7 157 2.34e-12 64.7
MS.gene72494.t1 MTR_8g023930 31.373 153 89 6 2 144 22 168 2.71e-12 65.1
MS.gene72494.t1 MTR_4g098630 34.559 136 72 8 4 129 6 134 2.90e-12 64.7
MS.gene72494.t1 MTR_5g069030 32.500 160 79 10 4 145 25 173 3.53e-12 65.1
MS.gene72494.t1 MTR_8g059170 30.380 158 85 7 4 145 14 162 7.88e-12 63.5
MS.gene72494.t1 MTR_2g079990 28.177 181 99 8 4 162 16 187 9.38e-12 63.5
MS.gene72494.t1 MTR_8g467490 28.743 167 89 8 2 145 12 171 1.05e-11 63.5
MS.gene72494.t1 MTR_7g085220 28.743 167 87 7 4 145 8 167 1.39e-11 62.8
MS.gene72494.t1 MTR_1g090720 32.099 162 83 7 4 145 42 196 1.68e-11 62.8
MS.gene72494.t1 MTR_3g088110 28.931 159 88 6 4 146 7 156 1.76e-11 62.4
MS.gene72494.t1 MTR_4g075980 29.333 150 89 6 6 145 17 159 1.86e-11 62.4
MS.gene72494.t1 MTR_6g084430 30.968 155 88 7 2 145 3 149 2.37e-11 62.0
MS.gene72494.t1 MTR_7g005280 30.968 155 83 7 4 145 15 158 2.77e-11 61.6
MS.gene72494.t1 MTR_4g108760 31.210 157 85 8 4 145 17 165 2.94e-11 62.0
MS.gene72494.t1 MTR_4g052620 30.061 163 90 7 2 145 3 160 3.66e-11 61.2
MS.gene72494.t1 MTR_2g086690 27.329 161 92 7 5 145 5 160 5.57e-11 60.5
MS.gene72494.t1 MTR_8g093580 30.769 156 87 7 4 145 6 154 7.18e-11 61.2
MS.gene72494.t1 MTR_4g094302 32.283 127 76 5 4 129 11 128 8.98e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72494.t1 AT2G27300 31.724 145 90 4 4 144 14 153 1.05e-17 80.1
MS.gene72494.t1 AT4G36160 33.117 154 85 6 4 145 14 161 3.87e-16 76.3
MS.gene72494.t1 AT4G36160 33.117 154 85 6 4 145 10 157 3.88e-16 76.3
MS.gene72494.t1 AT4G36160 33.117 154 85 6 4 145 10 157 3.88e-16 76.3
MS.gene72494.t1 AT2G24430 34.395 157 83 7 2 145 14 163 4.73e-16 75.5
MS.gene72494.t1 AT2G24430 34.395 157 83 7 2 145 14 163 4.73e-16 75.5
MS.gene72494.t1 AT1G71930 31.410 156 91 5 4 148 9 159 7.00e-16 75.1
MS.gene72494.t1 AT1G71930 31.410 156 91 5 4 148 9 159 7.00e-16 75.1
MS.gene72494.t1 AT3G44290 31.081 148 83 6 6 144 16 153 7.82e-16 73.6
MS.gene72494.t1 AT1G62700 32.692 156 83 7 4 145 7 154 2.68e-15 73.9
MS.gene72494.t1 AT1G62700 32.692 156 83 7 4 145 7 154 2.68e-15 73.9
MS.gene72494.t1 AT3G44290 29.655 145 89 5 6 144 16 153 4.84e-15 72.8
MS.gene72494.t1 AT4G17980 32.278 158 84 7 4 144 6 157 1.10e-14 70.9
MS.gene72494.t1 AT4G17980 32.278 158 84 7 4 144 6 157 1.19e-14 71.2
MS.gene72494.t1 AT1G61110 34.848 132 73 4 4 129 16 140 2.27e-14 70.9
MS.gene72494.t1 AT5G22290 30.822 146 87 6 6 144 23 161 2.76e-14 70.9
MS.gene72494.t1 AT1G65910 32.484 157 83 8 4 145 6 154 3.55e-14 70.9
MS.gene72494.t1 AT1G54330 30.921 152 89 6 4 145 6 151 4.09e-14 70.1
MS.gene72494.t1 AT4G35580 30.247 162 92 7 1 148 6 160 5.03e-14 70.5
MS.gene72494.t1 AT4G35580 29.944 177 101 8 1 161 6 175 5.04e-14 70.5
MS.gene72494.t1 AT1G32510 31.515 165 82 8 3 144 5 161 5.12e-14 69.3
MS.gene72494.t1 AT4G35580 30.247 162 92 7 1 148 6 160 5.17e-14 70.5
MS.gene72494.t1 AT5G46590 32.331 133 77 5 4 129 6 132 5.55e-14 69.3
MS.gene72494.t1 AT1G26870 31.818 154 91 5 2 148 14 160 7.34e-14 69.7
MS.gene72494.t1 AT5G24590 30.573 157 87 5 4 145 13 162 2.12e-13 68.6
MS.gene72494.t1 AT1G69490 31.250 176 96 8 4 162 9 176 2.29e-13 67.4
MS.gene72494.t1 AT4G27410 29.814 161 90 7 4 150 14 165 2.41e-13 67.8
MS.gene72494.t1 AT3G18400 31.847 157 87 8 2 146 3 151 2.55e-13 67.8
MS.gene72494.t1 AT1G76420 30.818 159 84 7 4 146 22 170 6.21e-13 66.6
MS.gene72494.t1 AT5G39610 31.210 157 85 7 4 145 20 168 7.11e-13 66.2
MS.gene72494.t1 AT5G64060 29.680 219 122 10 4 205 6 209 1.08e-12 66.2
MS.gene72494.t1 AT2G43000 32.468 154 82 8 6 145 20 165 1.32e-12 65.5
MS.gene72494.t1 AT5G39820 33.835 133 70 6 6 129 22 145 1.61e-12 65.5
MS.gene72494.t1 AT1G52890 30.769 156 85 7 4 145 14 160 1.74e-12 65.5
MS.gene72494.t1 AT3G15500 30.323 155 87 7 4 145 14 160 2.05e-12 65.1
MS.gene72494.t1 AT5G04410 30.968 155 88 6 4 145 9 157 2.50e-12 65.5
MS.gene72494.t1 AT5G09330 30.968 155 88 6 4 145 6 154 3.25e-12 65.1
MS.gene72494.t1 AT5G09330 30.968 155 88 6 4 145 6 154 3.25e-12 65.1
MS.gene72494.t1 AT5G09330 30.968 155 88 6 4 145 6 154 3.25e-12 65.1
MS.gene72494.t1 AT5G09330 30.968 155 88 6 4 145 6 154 3.25e-12 65.1
MS.gene72494.t1 AT5G09330 30.968 155 88 6 4 145 6 154 3.25e-12 65.1
MS.gene72494.t1 AT3G29035 32.576 132 75 5 4 129 24 147 5.09e-12 63.9
MS.gene72494.t1 AT2G33480 32.680 153 83 7 4 145 15 158 1.14e-11 62.8
MS.gene72494.t1 AT1G34190 29.936 157 91 6 2 145 14 164 1.50e-11 63.2
MS.gene72494.t1 AT4G27410 27.429 175 93 7 4 150 14 182 2.48e-11 62.0
MS.gene72494.t1 AT5G61430 30.247 162 90 7 4 150 16 169 2.61e-11 62.0
MS.gene72494.t1 AT5G64530 28.351 194 104 7 4 175 3 183 4.20e-11 60.1
MS.gene72494.t1 AT3G04060 28.342 187 110 7 4 174 20 198 5.49e-11 61.2
MS.gene72494.t1 AT1G32870 32.061 131 79 5 4 130 10 134 5.58e-11 61.6
MS.gene72494.t1 AT1G32870 32.061 131 79 5 4 130 10 134 5.87e-11 61.6
MS.gene72494.t1 AT1G32870 32.061 131 79 5 4 130 46 170 8.26e-11 61.2
MS.gene72494.t1 AT5G18270 30.380 158 86 7 4 145 21 170 9.20e-11 60.5
MS.gene72494.t1 AT2G17040 29.747 158 78 8 6 145 8 150 9.28e-11 60.1
MS.gene72494.t1 AT5G13180 31.169 154 85 7 4 145 14 158 9.50e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATCTGGACTCTGAATT+TGG 0.253239 4.2:-69246742 MS.gene72494:CDS
CAATGGTTCTGACCTGATTA+AGG 0.258535 4.2:-69247202 MS.gene72494:CDS
CTCAGCAACTTAAGCGGGCT+GGG 0.289500 4.2:-69246651 MS.gene72494:CDS
CAGAGGAGGCATTGTAAGAT+TGG 0.351545 4.2:-69247075 MS.gene72494:CDS
GGCATTGTAAGATTGGGTTT+TGG 0.353732 4.2:-69247068 MS.gene72494:CDS
ATAAGTTCAGCTCCTTAATC+AGG 0.404146 4.2:+69247190 None:intergenic
CAACTTAAGCGGGCTGGGGC+TGG 0.410453 4.2:-69246646 MS.gene72494:CDS
GAAGAGGTTCAGAATTTCAA+TGG 0.424440 4.2:-69247219 MS.gene72494:CDS
CCTCCTCTGCTCATCAACTT+TGG 0.447718 4.2:+69247089 None:intergenic
GGACTAAGAGGCAATTCTAA+TGG 0.448627 4.2:+69247156 None:intergenic
AAAACGCGGCAAACAACAAA+AGG 0.449123 4.2:+69246781 None:intergenic
AGAGGAGGCATTGTAAGATT+GGG 0.450614 4.2:-69247074 MS.gene72494:CDS
TGGAGTTGATAACGTGGTTG+TGG 0.453423 4.2:-69247016 MS.gene72494:CDS
GAATTCCTTCCACCTGGTGT+TGG 0.454396 4.2:-69247297 MS.gene72494:CDS
ACTCAGCAACTTAAGCGGGC+TGG 0.460490 4.2:-69246652 MS.gene72494:CDS
CTGCCCTAGAGGGATGGATC+TGG 0.463977 4.2:-69246755 MS.gene72494:CDS
TTCGATGACAAGGCGAATGA+AGG 0.469350 4.2:-69246676 MS.gene72494:CDS
GCAATTCTAATGGATCATAC+TGG 0.492236 4.2:+69247166 None:intergenic
GAGTCCAGATCCATCCCTCT+AGG 0.508074 4.2:+69246751 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 4.2:+69247114 None:intergenic
ACCTTCATCGACATCAGAAA+AGG 0.512248 4.2:+69246717 None:intergenic
GGAGTTGATAACGTGGTTGT+GGG 0.517524 4.2:-69247015 MS.gene72494:CDS
GGAGTTGTTCGAGACATTTG+TGG 0.527780 4.2:-69247036 MS.gene72494:CDS
GGCTACTCAAGCCACTCCTT+AGG 0.532323 4.2:-69246587 None:intergenic
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 4.2:+69247121 None:intergenic
TAACAAGGAGCTCATCTGAA+GGG 0.545729 4.2:+69247268 None:intergenic
AGAACGCGATCAAAACTAGC+TGG 0.546737 4.2:-69246951 MS.gene72494:CDS
AGGAACTCAGCAACTTAAGC+GGG 0.556951 4.2:-69246656 MS.gene72494:CDS
AGGAGTGGCTTGAGTAGCCA+TGG 0.558328 4.2:+69246591 None:intergenic
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 4.2:+69247115 None:intergenic
GAAGGGGCGAAGCCAACACC+AGG 0.562826 4.2:+69247285 None:intergenic
CGAAGCCAACACCAGGTGGA+AGG 0.569669 4.2:+69247292 None:intergenic
AGGCTATGCATCGCATTCCA+TGG 0.571087 4.2:-69246608 MS.gene72494:CDS
CCAAAGTTGATGAGCAGAGG+AGG 0.574327 4.2:-69247089 MS.gene72494:CDS
TCAGCAACTTAAGCGGGCTG+GGG 0.577406 4.2:-69246650 MS.gene72494:CDS
AGTCCAGATCCATCCCTCTA+GGG 0.585447 4.2:+69246752 None:intergenic
CACTCAGGTTAACAACCCTC+AGG 0.585811 4.2:-69246914 MS.gene72494:intron
CTAACAAGGAGCTCATCTGA+AGG 0.597005 4.2:+69247267 None:intergenic
GCTGGTTCTAGTGCTGAGCA+AGG 0.601917 4.2:-69246628 MS.gene72494:CDS
GCAATGGAATTCCTTCCACC+TGG 0.603855 4.2:-69247303 MS.gene72494:CDS
TGGCAAGGTAGTAGCTAACA+AGG 0.615826 4.2:+69247253 None:intergenic
AACAAGGAGCTCATCTGAAG+GGG 0.618835 4.2:+69247269 None:intergenic
CTGCCAAAGTTGATGAGCAG+AGG 0.624165 4.2:-69247092 MS.gene72494:CDS
AAGGAACTCAGCAACTTAAG+CGG 0.627875 4.2:-69246657 MS.gene72494:CDS
TGATGACGACTTCGATGACA+AGG 0.631139 4.2:-69246686 MS.gene72494:CDS
GGGGCGAAGCCAACACCAGG+TGG 0.632420 4.2:+69247288 None:intergenic
GACTATGTACGAGTACACTC+AGG 0.643748 4.2:-69246929 MS.gene72494:CDS
CATTTGTGGAGTTGATAACG+TGG 0.688917 4.2:-69247022 MS.gene72494:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATATAAATAATTATGC+AGG - chr4.2:69247082-69247101 MS.gene72494:CDS 10.0%
!! TCGATGATTAATTTATGTAT+TGG - chr4.2:69247041-69247060 MS.gene72494:CDS 20.0%
!!! TCTCTTTTTATGTTCTATAT+AGG - chr4.2:69246903-69246922 MS.gene72494:intron 20.0%
! TCGATGAAATAATGAACATA+TGG + chr4.2:69247025-69247044 None:intergenic 25.0%
!! AAGAAAAATGATGGACTAAG+AGG + chr4.2:69246747-69246766 None:intergenic 30.0%
!!! AACCTCTTCATTGTTTTTGT+TGG + chr4.2:69246658-69246677 None:intergenic 30.0%
ATAAGTTCAGCTCCTTAATC+AGG + chr4.2:69246701-69246720 None:intergenic 35.0%
GAAGAGGTTCAGAATTTCAA+TGG - chr4.2:69246669-69246688 MS.gene72494:CDS 35.0%
TGAAGCAGTAAAATTCCCTT+GGG + chr4.2:69246776-69246795 None:intergenic 35.0%
TGCCAACAAAAACAATGAAG+AGG - chr4.2:69246653-69246672 MS.gene72494:CDS 35.0%
! GCAATTCTAATGGATCATAC+TGG + chr4.2:69246725-69246744 None:intergenic 35.0%
!!! CTTCATTGTTTTTGTTGGCA+AGG + chr4.2:69246653-69246672 None:intergenic 35.0%
AAAACGCGGCAAACAACAAA+AGG + chr4.2:69247110-69247129 None:intergenic 40.0%
AAGGAACTCAGCAACTTAAG+CGG - chr4.2:69247231-69247250 MS.gene72494:CDS 40.0%
ACCTTCATCGACATCAGAAA+AGG + chr4.2:69247174-69247193 None:intergenic 40.0%
AGTAAAATTCCCTTGGGTGA+CGG + chr4.2:69246770-69246789 None:intergenic 40.0%
ATGGATCTGGACTCTGAATT+TGG - chr4.2:69247146-69247165 MS.gene72494:CDS 40.0%
GGACTAAGAGGCAATTCTAA+TGG + chr4.2:69246735-69246754 None:intergenic 40.0%
GTGAAGCAGTAAAATTCCCT+TGG + chr4.2:69246777-69246796 None:intergenic 40.0%
TAACAAGGAGCTCATCTGAA+GGG + chr4.2:69246623-69246642 None:intergenic 40.0%
TTCCCAAATGACATACCTGA+GGG + chr4.2:69246992-69247011 None:intergenic 40.0%
TTTCCCAAATGACATACCTG+AGG + chr4.2:69246993-69247012 None:intergenic 40.0%
! AACCCTCAGGTATGTCATTT+GGG - chr4.2:69246987-69247006 MS.gene72494:CDS 40.0%
! ATTTTTCTTCCGTCACCCAA+GGG - chr4.2:69246758-69246777 MS.gene72494:CDS 40.0%
! GGCATTGTAAGATTGGGTTT+TGG - chr4.2:69246820-69246839 MS.gene72494:intron 40.0%
!! AGAGGAGGCATTGTAAGATT+GGG - chr4.2:69246814-69246833 MS.gene72494:intron 40.0%
!! CAATGGTTCTGACCTGATTA+AGG - chr4.2:69246686-69246705 MS.gene72494:CDS 40.0%
!! CATTTGTGGAGTTGATAACG+TGG - chr4.2:69246866-69246885 MS.gene72494:intron 40.0%
!!! ATTGGGTTTTGGATGCGTAA+GGG - chr4.2:69246831-69246850 MS.gene72494:intron 40.0%
!!! TAGTTTTGATCGCGTTCTGA+GGG + chr4.2:69246935-69246954 None:intergenic 40.0%
AACAAGGAGCTCATCTGAAG+GGG + chr4.2:69246622-69246641 None:intergenic 45.0%
AGAACGCGATCAAAACTAGC+TGG - chr4.2:69246937-69246956 MS.gene72494:CDS 45.0%
AGGAACTCAGCAACTTAAGC+GGG - chr4.2:69247232-69247251 MS.gene72494:CDS 45.0%
CAACCCTCAGGTATGTCATT+TGG - chr4.2:69246986-69247005 MS.gene72494:CDS 45.0%
CTAACAAGGAGCTCATCTGA+AGG + chr4.2:69246624-69246643 None:intergenic 45.0%
GACTATGTACGAGTACACTC+AGG - chr4.2:69246959-69246978 MS.gene72494:CDS 45.0%
GGAGTTGTTCGAGACATTTG+TGG - chr4.2:69246852-69246871 MS.gene72494:intron 45.0%
TGGCAAGGTAGTAGCTAACA+AGG + chr4.2:69246638-69246657 None:intergenic 45.0%
TTCGATGACAAGGCGAATGA+AGG - chr4.2:69247212-69247231 MS.gene72494:CDS 45.0%
! CATTTTTCTTCCGTCACCCA+AGG - chr4.2:69246757-69246776 MS.gene72494:CDS 45.0%
! GCCTTTTCTGATGTCGATGA+AGG - chr4.2:69247170-69247189 MS.gene72494:CDS 45.0%
! TGATGACGACTTCGATGACA+AGG - chr4.2:69247202-69247221 MS.gene72494:CDS 45.0%
!! AGTTTTGATCGCGTTCTGAG+GGG + chr4.2:69246934-69246953 None:intergenic 45.0%
!! CAGAGGAGGCATTGTAAGAT+TGG - chr4.2:69246813-69246832 MS.gene72494:intron 45.0%
!! GGAGTTGATAACGTGGTTGT+GGG - chr4.2:69246873-69246892 MS.gene72494:intron 45.0%
!! GGGTGACGGAAGAAAAATGA+TGG + chr4.2:69246756-69246775 None:intergenic 45.0%
!! TGGAGTTGATAACGTGGTTG+TGG - chr4.2:69246872-69246891 MS.gene72494:intron 45.0%
!!! CTAGTTTTGATCGCGTTCTG+AGG + chr4.2:69246936-69246955 None:intergenic 45.0%
!!! GATTGGGTTTTGGATGCGTA+AGG - chr4.2:69246830-69246849 MS.gene72494:intron 45.0%
AGGCTATGCATCGCATTCCA+TGG - chr4.2:69247280-69247299 MS.gene72494:CDS 50.0%
AGTCCAGATCCATCCCTCTA+GGG + chr4.2:69247139-69247158 None:intergenic 50.0%
CACTCAGGTTAACAACCCTC+AGG - chr4.2:69246974-69246993 MS.gene72494:CDS 50.0%
CCTCCTCTGCTCATCAACTT+TGG + chr4.2:69246802-69246821 None:intergenic 50.0%
GAATTCCTTCCACCTGGTGT+TGG - chr4.2:69246591-69246610 MS.gene72494:CDS 50.0%
!! CCAAAGTTGATGAGCAGAGG+AGG - chr4.2:69246799-69246818 MS.gene72494:intron 50.0%
!! CTCTAGGGCAGTCAAAAACG+CGG + chr4.2:69247124-69247143 None:intergenic 50.0%
!! CTGCCAAAGTTGATGAGCAG+AGG - chr4.2:69246796-69246815 MS.gene72494:intron 50.0%
!! GCGTTTTTGACTGCCCTAGA+GGG - chr4.2:69247123-69247142 MS.gene72494:CDS 50.0%
!! TTTTGACTGCCCTAGAGGGA+TGG - chr4.2:69247127-69247146 MS.gene72494:CDS 50.0%
ACTCAGCAACTTAAGCGGGC+TGG - chr4.2:69247236-69247255 MS.gene72494:CDS 55.0%
AGGAGTGGCTTGAGTAGCCA+TGG + chr4.2:69247300-69247319 None:intergenic 55.0%
GAGTCCAGATCCATCCCTCT+AGG + chr4.2:69247140-69247159 None:intergenic 55.0%
! CTCAGCAACTTAAGCGGGCT+GGG - chr4.2:69247237-69247256 MS.gene72494:CDS 55.0%
!! CGCGTTTTTGACTGCCCTAG+AGG - chr4.2:69247122-69247141 MS.gene72494:CDS 55.0%
!! GCTGGTTCTAGTGCTGAGCA+AGG - chr4.2:69247260-69247279 MS.gene72494:CDS 55.0%
!! TCAGCAACTTAAGCGGGCTG+GGG - chr4.2:69247238-69247257 MS.gene72494:CDS 55.0%
CGAAGCCAACACCAGGTGGA+AGG + chr4.2:69246599-69246618 None:intergenic 60.0%
CTGCCCTAGAGGGATGGATC+TGG - chr4.2:69247133-69247152 MS.gene72494:CDS 60.0%
GAAGGGGCGAAGCCAACACC+AGG + chr4.2:69246606-69246625 None:intergenic 65.0%
!! CAACTTAAGCGGGCTGGGGC+TGG - chr4.2:69247242-69247261 MS.gene72494:CDS 65.0%
GGGGCGAAGCCAACACCAGG+TGG + chr4.2:69246603-69246622 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 69246588 69247322 69246588 ID=MS.gene72494
chr4.2 mRNA 69246588 69247322 69246588 ID=MS.gene72494.t1;Parent=MS.gene72494
chr4.2 exon 69246915 69247322 69246915 ID=MS.gene72494.t1.exon1;Parent=MS.gene72494.t1
chr4.2 CDS 69246915 69247322 69246915 ID=cds.MS.gene72494.t1;Parent=MS.gene72494.t1
chr4.2 exon 69246588 69246806 69246588 ID=MS.gene72494.t1.exon2;Parent=MS.gene72494.t1
chr4.2 CDS 69246588 69246806 69246588 ID=cds.MS.gene72494.t1;Parent=MS.gene72494.t1
Gene Sequence

>MS.gene72494

ATGGAATTCCTTCCACCTGGTGTTGGCTTCGCCCCTTCAGATGAGCTCCTTGTTAGCTACTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCAATGGTTCTGACCTGATTAAGGAGCTGAACTTATACCAGTATGATCCATTAGAATTGCCTCTTAGTCCATCATTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGAGGAGGCATTGTAAGATTGGGTTTTGGATGCGTAAGGGAGTTGTTCGAGACATTTGTGGAGTTGATAACGTGGTTGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGCTGGACTATGTACGAGTACACTCAGGTTAACAACCCTCAGGTATGTCATTTGGGAAAACTAAGTTTCCATATGTTCATTATTTCATCGATGATTAATTTATGTATTGGTAACGACAAACTTTTTTTTTTAATATAAATAATTATGCAGGCTCCTTTTGTTGTTTGCCGCGTTTTTGACTGCCCTAGAGGGATGGATCTGGACTCTGAATTTGGAGCCTTTTCTGATGTCGATGAAGGTGATGCTGCTGATGACGACTTCGATGACAAGGCGAATGAAGGAACTCAGCAACTTAAGCGGGCTGGGGCTGGTTCTAGTGCTGAGCAAGGCTATGCATCGCATTCCATGGCTACTCAAGCCACTCCTTAG
Protein sequence

>MS.gene72494.t1

MEFLPPGVGFAPSDELLVSYYLANKNNEEVQNFNGSDLIKELNLYQYDPLELPLSPSFFFRHPREFYCFTAKVDEQRRHCKIGFWMRKGVVRDICGVDNVVVGKKSLFMFYIGNSPQNAIKTSWTMYEYTQVNNPQAPFVVCRVFDCPRGMDLDSEFGAFSDVDEGDAADDDFDDKANEGTQQLKRAGAGSSAEQGYASHSMATQATP