Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72550.t1 | XP_024636235.1 | 94.7 | 243 | 13 | 0 | 1 | 243 | 1 | 243 | 1.30E-111 | 412.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72550.t1 | Q9M202 | 27.8 | 194 | 133 | 4 | 9 | 196 | 6 | 198 | 1.6e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72550.t1 | A0A396I2K7 | 66.9 | 245 | 75 | 1 | 1 | 239 | 1 | 245 | 7.2e-67 | 263.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene72550.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72550.t1 | MTR_4g036615 | 77.885 | 104 | 23 | 0 | 78 | 181 | 1 | 104 | 9.50e-55 | 171 |
| MS.gene72550.t1 | MTR_3g408380 | 28.302 | 265 | 146 | 7 | 3 | 229 | 2 | 260 | 1.34e-21 | 90.9 |
| MS.gene72550.t1 | MTR_3g408420 | 26.165 | 279 | 138 | 7 | 7 | 232 | 6 | 269 | 4.47e-18 | 81.3 |
| MS.gene72550.t1 | MTR_3g008310 | 26.415 | 265 | 157 | 7 | 3 | 229 | 2 | 266 | 5.00e-16 | 75.9 |
| MS.gene72550.t1 | MTR_4g100860 | 59.322 | 59 | 22 | 1 | 173 | 229 | 210 | 268 | 2.94e-13 | 68.6 |
| MS.gene72550.t1 | MTR_0160s0030 | 40.741 | 81 | 38 | 1 | 170 | 240 | 239 | 319 | 9.03e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72550.t1 | AT3G60580 | 30.400 | 250 | 136 | 7 | 9 | 229 | 6 | 246 | 6.03e-18 | 81.3 |
| MS.gene72550.t1 | AT1G02030 | 27.132 | 258 | 132 | 6 | 9 | 236 | 7 | 238 | 3.94e-13 | 67.4 |
| MS.gene72550.t1 | AT5G61470 | 41.096 | 73 | 31 | 2 | 168 | 229 | 225 | 296 | 3.84e-12 | 65.1 |
Find 66 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGCAATTGGATCTCTTGC+GGG | 0.303465 | 4.4:-66832364 | None:intergenic |
| AATTGGATCTCTTGCGGGTT+GGG | 0.313326 | 4.4:-66832359 | None:intergenic |
| ATCCAGGTGTTCTGATGTTA+TGG | 0.324938 | 4.4:+66832430 | MS.gene72550:CDS |
| TGAGTATTAGTTTCAAGCTT+TGG | 0.337317 | 4.4:-66832165 | None:intergenic |
| CAGCCGCAGCATTAACTTCT+CGG | 0.371873 | 4.4:-66832386 | None:intergenic |
| CAATTGGATCTCTTGCGGGT+TGG | 0.402481 | 4.4:-66832360 | None:intergenic |
| ATTAACTTCTCGGCCGCAAT+TGG | 0.433182 | 4.4:-66832376 | None:intergenic |
| TACTCAAGCTGTAGACAATT+CGG | 0.436960 | 4.4:+66832182 | MS.gene72550:CDS |
| ATTTGTGGGAAGTGGTTCTC+CGG | 0.453707 | 4.4:+66832087 | MS.gene72550:CDS |
| GTTTCAAGCTTTGGAGCAAT+CGG | 0.454553 | 4.4:-66832156 | None:intergenic |
| TTCCGATCTTATCAGGCTCT+TGG | 0.457389 | 4.4:+66832594 | MS.gene72550:CDS |
| TTTACAAAGATGCGCAGAAA+AGG | 0.459521 | 4.4:+66832457 | MS.gene72550:CDS |
| TACAGAATCACAAATCAATA+CGG | 0.462697 | 4.4:-66832780 | None:intergenic |
| TTCTCCGGTGGGAAAGCCCT+TGG | 0.463478 | 4.4:+66832102 | MS.gene72550:CDS |
| GGAAAGCCTGTGGGATTTGT+GGG | 0.467351 | 4.4:+66832073 | MS.gene72550:CDS |
| GGGAAAGCCTGTGGGATTTG+TGG | 0.468727 | 4.4:+66832072 | MS.gene72550:CDS |
| CGATCTTATCAGGCTCTTGG+TGG | 0.468794 | 4.4:+66832597 | MS.gene72550:CDS |
| TTGCAATTTGAGCAGTGAAA+AGG | 0.471865 | 4.4:-66832672 | None:intergenic |
| GCCTCCAAGGGCTTTCCCAC+CGG | 0.472807 | 4.4:-66832106 | None:intergenic |
| CCCCATAACATCAGAACACC+TGG | 0.474579 | 4.4:-66832432 | None:intergenic |
| AGATCTCATGTGGCCTCCAA+GGG | 0.485485 | 4.4:-66832118 | None:intergenic |
| GGCCGCAATTGGATCTCTTG+CGG | 0.489489 | 4.4:-66832365 | None:intergenic |
| TCCAGGTGTTCTGATGTTAT+GGG | 0.494593 | 4.4:+66832431 | MS.gene72550:CDS |
| TCCGGTGGGAAAGCCCTTGG+AGG | 0.494800 | 4.4:+66832105 | MS.gene72550:CDS |
| TTCTCTGTTTGAGCTTGCAG+AGG | 0.496954 | 4.4:-66832310 | None:intergenic |
| TCTGTTTGAGCTTGCAGAGG+AGG | 0.498454 | 4.4:-66832307 | None:intergenic |
| TTACAAAGATGCGCAGAAAA+GGG | 0.499625 | 4.4:+66832458 | MS.gene72550:CDS |
| TTCAAGTCTGCTCAGGCACT+CGG | 0.500253 | 4.4:+66832702 | MS.gene72550:CDS |
| AACGATGGCGAAAGCAGTAC+AGG | 0.507873 | 4.4:+66832510 | MS.gene72550:CDS |
| AGCAAATTGATTTCGACGAT+GGG | 0.509489 | 4.4:-66832214 | None:intergenic |
| GCACCTCTCACAGTTGAACC+TGG | 0.509618 | 4.4:-66832562 | None:intergenic |
| ATTTCGACGATGGGTTGAAT+CGG | 0.510947 | 4.4:-66832205 | None:intergenic |
| GAGATCTCATGTGGCCTCCA+AGG | 0.514030 | 4.4:-66832119 | None:intergenic |
| GAAGATATGTCAAGAGATCC+AGG | 0.516117 | 4.4:+66832414 | MS.gene72550:CDS |
| TGTGGGAAGTGGTTCTCCGG+TGG | 0.517822 | 4.4:+66832090 | MS.gene72550:CDS |
| ACTTTGAAATTCCTTAGCAC+TGG | 0.521740 | 4.4:-66832748 | None:intergenic |
| CAAAGTGTTCAAGTCTGCTC+AGG | 0.524975 | 4.4:+66832695 | MS.gene72550:CDS |
| CACAAGCAGACTCTGAAGCC+AGG | 0.539423 | 4.4:+66832544 | MS.gene72550:CDS |
| AAGCAAATTGATTTCGACGA+TGG | 0.551421 | 4.4:-66832215 | None:intergenic |
| GTGTTTGTTGTATTTCGGGA+AGG | 0.552910 | 4.4:-66832251 | None:intergenic |
| CCTGTGGGATTTGTGGGAAG+TGG | 0.558125 | 4.4:+66832079 | MS.gene72550:CDS |
| GTGGGAAGTGGTTCTCCGGT+GGG | 0.561353 | 4.4:+66832091 | MS.gene72550:CDS |
| GATCTTATCAGGCTCTTGGT+GGG | 0.571526 | 4.4:+66832598 | MS.gene72550:CDS |
| GGTACACTCTTCCAGTGCTA+AGG | 0.575791 | 4.4:+66832737 | MS.gene72550:CDS |
| GCAACTATGGAGAAGAGCGA+TGG | 0.579019 | 4.4:+66832051 | None:intergenic |
| AAAGATGCGCAGAAAAGGGA+AGG | 0.579501 | 4.4:+66832462 | MS.gene72550:CDS |
| CACCAAGAGCCTGATAAGAT+CGG | 0.582662 | 4.4:-66832596 | None:intergenic |
| CCAGGTGTTCTGATGTTATG+GGG | 0.595199 | 4.4:+66832432 | MS.gene72550:CDS |
| AAGAGCGATGGGAAAGCCTG+TGG | 0.598960 | 4.4:+66832063 | MS.gene72550:CDS |
| AGGCACTCGGTGGACACAAG+AGG | 0.606213 | 4.4:+66832715 | MS.gene72550:CDS |
| ACAGAATCACAAATCAATAC+GGG | 0.613334 | 4.4:-66832779 | None:intergenic |
| CAACTATGGAGAAGAGCGAT+GGG | 0.616240 | 4.4:+66832052 | None:intergenic |
| TACTGCAAAAGAGACAATGA+TGG | 0.621835 | 4.4:+66832630 | MS.gene72550:CDS |
| AAGCCAGGTTCAACTGTGAG+AGG | 0.622627 | 4.4:+66832559 | MS.gene72550:CDS |
| ATCACAAATCAATACGGGTG+TGG | 0.631203 | 4.4:-66832774 | None:intergenic |
| GGCACTCGGTGGACACAAGA+GGG | 0.632635 | 4.4:+66832716 | MS.gene72550:CDS |
| CCACTTCCCACAAATCCCAC+AGG | 0.647858 | 4.4:-66832079 | None:intergenic |
| CGGCCGAGAAGTTAATGCTG+CGG | 0.653910 | 4.4:+66832383 | MS.gene72550:CDS |
| AGAGCGATGGGAAAGCCTGT+GGG | 0.654994 | 4.4:+66832064 | MS.gene72550:CDS |
| AGGTTCAACTGTGAGAGGTG+CGG | 0.663022 | 4.4:+66832564 | MS.gene72550:CDS |
| AAGATGCGCAGAAAAGGGAA+GGG | 0.667779 | 4.4:+66832463 | MS.gene72550:CDS |
| TTCGGGAAGGCAAGTCTGTG+AGG | 0.669270 | 4.4:-66832238 | None:intergenic |
| AAGTCTGCTCAGGCACTCGG+TGG | 0.670340 | 4.4:+66832705 | MS.gene72550:CDS |
| AGGCCACATGAGATCTCACA+TGG | 0.682156 | 4.4:+66832125 | MS.gene72550:CDS |
| ACCCGCAAGAGATCCAATTG+CGG | 0.686880 | 4.4:+66832363 | MS.gene72550:CDS |
| GAACCATGTGAGATCTCATG+TGG | 0.730676 | 4.4:-66832128 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGTGTTTGTTGTATTT+CGG | - | chr4.4:66832259-66832278 | None:intergenic | 20.0% |
| ! | GATCAAGAAACAAAAAATGA+GGG | + | chr4.4:66832485-66832504 | MS.gene72550:CDS | 25.0% |
| !!! | TTTTGTGTTTGTTGTATTTC+GGG | - | chr4.4:66832258-66832277 | None:intergenic | 25.0% |
| AAAAATGTTCCGATCTTATC+AGG | + | chr4.4:66832587-66832606 | MS.gene72550:CDS | 30.0% | |
| GGATCAAGAAACAAAAAATG+AGG | + | chr4.4:66832484-66832503 | MS.gene72550:CDS | 30.0% | |
| ! | TGAGTATTAGTTTCAAGCTT+TGG | - | chr4.4:66832168-66832187 | None:intergenic | 30.0% |
| !!! | TGAAGTTTTGTTTCAAGGAT+AGG | - | chr4.4:66832284-66832303 | None:intergenic | 30.0% |
| CAAAAAATGAGGGAAAACGA+TGG | + | chr4.4:66832495-66832514 | MS.gene72550:CDS | 35.0% | |
| TACTCAAGCTGTAGACAATT+CGG | + | chr4.4:66832182-66832201 | MS.gene72550:CDS | 35.0% | |
| TACTGCAAAAGAGACAATGA+TGG | + | chr4.4:66832630-66832649 | MS.gene72550:CDS | 35.0% | |
| TTACAAAGATGCGCAGAAAA+GGG | + | chr4.4:66832458-66832477 | MS.gene72550:CDS | 35.0% | |
| TTGCAATTTGAGCAGTGAAA+AGG | - | chr4.4:66832675-66832694 | None:intergenic | 35.0% | |
| TTTACAAAGATGCGCAGAAA+AGG | + | chr4.4:66832457-66832476 | MS.gene72550:CDS | 35.0% | |
| ! | AAGCAAATTGATTTCGACGA+TGG | - | chr4.4:66832218-66832237 | None:intergenic | 35.0% |
| ! | AGCAAATTGATTTCGACGAT+GGG | - | chr4.4:66832217-66832236 | None:intergenic | 35.0% |
| !! | ACTTTGAAATTCCTTAGCAC+TGG | - | chr4.4:66832751-66832770 | None:intergenic | 35.0% |
| ATCCAGGTGTTCTGATGTTA+TGG | + | chr4.4:66832430-66832449 | MS.gene72550:CDS | 40.0% | |
| GAAGATATGTCAAGAGATCC+AGG | + | chr4.4:66832414-66832433 | MS.gene72550:CDS | 40.0% | |
| TCCAGGTGTTCTGATGTTAT+GGG | + | chr4.4:66832431-66832450 | MS.gene72550:CDS | 40.0% | |
| !! | ATTTCGACGATGGGTTGAAT+CGG | - | chr4.4:66832208-66832227 | None:intergenic | 40.0% |
| !! | GTGTTTGTTGTATTTCGGGA+AGG | - | chr4.4:66832254-66832273 | None:intergenic | 40.0% |
| !! | GTTTCAAGCTTTGGAGCAAT+CGG | - | chr4.4:66832159-66832178 | None:intergenic | 40.0% |
| !!! | GGCAGTGAAGTTTTGTTTCA+AGG | - | chr4.4:66832289-66832308 | None:intergenic | 40.0% |
| AAAGATGCGCAGAAAAGGGA+AGG | + | chr4.4:66832462-66832481 | MS.gene72550:CDS | 45.0% | |
| AAGATGCGCAGAAAAGGGAA+GGG | + | chr4.4:66832463-66832482 | MS.gene72550:CDS | 45.0% | |
| AATTGGATCTCTTGCGGGTT+GGG | - | chr4.4:66832362-66832381 | None:intergenic | 45.0% | |
| ATTAACTTCTCGGCCGCAAT+TGG | - | chr4.4:66832379-66832398 | None:intergenic | 45.0% | |
| ATTTGTGGGAAGTGGTTCTC+CGG | + | chr4.4:66832087-66832106 | MS.gene72550:CDS | 45.0% | |
| CACCAAGAGCCTGATAAGAT+CGG | - | chr4.4:66832599-66832618 | None:intergenic | 45.0% | |
| CCAGGTGTTCTGATGTTATG+GGG | + | chr4.4:66832432-66832451 | MS.gene72550:CDS | 45.0% | |
| GAACCATGTGAGATCTCATG+TGG | - | chr4.4:66832131-66832150 | None:intergenic | 45.0% | |
| GATCTTATCAGGCTCTTGGT+GGG | + | chr4.4:66832598-66832617 | MS.gene72550:CDS | 45.0% | |
| TTCCGATCTTATCAGGCTCT+TGG | + | chr4.4:66832594-66832613 | MS.gene72550:CDS | 45.0% | |
| TTCTCTGTTTGAGCTTGCAG+AGG | - | chr4.4:66832313-66832332 | None:intergenic | 45.0% | |
| ! | CAAAGTGTTCAAGTCTGCTC+AGG | + | chr4.4:66832695-66832714 | MS.gene72550:CDS | 45.0% |
| AACGATGGCGAAAGCAGTAC+AGG | + | chr4.4:66832510-66832529 | MS.gene72550:CDS | 50.0% | |
| AAGCCAGGTTCAACTGTGAG+AGG | + | chr4.4:66832559-66832578 | MS.gene72550:CDS | 50.0% | |
| ACCCGCAAGAGATCCAATTG+CGG | + | chr4.4:66832363-66832382 | MS.gene72550:CDS | 50.0% | |
| AGATCTCATGTGGCCTCCAA+GGG | - | chr4.4:66832121-66832140 | None:intergenic | 50.0% | |
| AGGCCACATGAGATCTCACA+TGG | + | chr4.4:66832125-66832144 | MS.gene72550:CDS | 50.0% | |
| AGGTTCAACTGTGAGAGGTG+CGG | + | chr4.4:66832564-66832583 | MS.gene72550:CDS | 50.0% | |
| CAATTGGATCTCTTGCGGGT+TGG | - | chr4.4:66832363-66832382 | None:intergenic | 50.0% | |
| CAGCCGCAGCATTAACTTCT+CGG | - | chr4.4:66832389-66832408 | None:intergenic | 50.0% | |
| CCCCATAACATCAGAACACC+TGG | - | chr4.4:66832435-66832454 | None:intergenic | 50.0% | |
| CGATCTTATCAGGCTCTTGG+TGG | + | chr4.4:66832597-66832616 | MS.gene72550:CDS | 50.0% | |
| GGAAAGCCTGTGGGATTTGT+GGG | + | chr4.4:66832073-66832092 | MS.gene72550:CDS | 50.0% | |
| GGTACACTCTTCCAGTGCTA+AGG | + | chr4.4:66832737-66832756 | MS.gene72550:CDS | 50.0% | |
| TCTGTTTGAGCTTGCAGAGG+AGG | - | chr4.4:66832310-66832329 | None:intergenic | 50.0% | |
| TTCAAGTCTGCTCAGGCACT+CGG | + | chr4.4:66832702-66832721 | MS.gene72550:CDS | 50.0% | |
| !!! | CTCTTGCGGGTTGGGTTTTT+CGG | - | chr4.4:66832354-66832373 | None:intergenic | 50.0% |
| !!! | TCTTGCGGGTTGGGTTTTTC+GGG | - | chr4.4:66832353-66832372 | None:intergenic | 50.0% |
| AAGAGCGATGGGAAAGCCTG+TGG | + | chr4.4:66832063-66832082 | MS.gene72550:CDS | 55.0% | |
| AGAGCGATGGGAAAGCCTGT+GGG | + | chr4.4:66832064-66832083 | MS.gene72550:CDS | 55.0% | |
| CCACTTCCCACAAATCCCAC+AGG | - | chr4.4:66832082-66832101 | None:intergenic | 55.0% | |
| CCTGTGGGATTTGTGGGAAG+TGG | + | chr4.4:66832079-66832098 | MS.gene72550:CDS | 55.0% | |
| CGGCCGAGAAGTTAATGCTG+CGG | + | chr4.4:66832383-66832402 | MS.gene72550:CDS | 55.0% | |
| GAGATCTCATGTGGCCTCCA+AGG | - | chr4.4:66832122-66832141 | None:intergenic | 55.0% | |
| GCACCTCTCACAGTTGAACC+TGG | - | chr4.4:66832565-66832584 | None:intergenic | 55.0% | |
| GCCGCAATTGGATCTCTTGC+GGG | - | chr4.4:66832367-66832386 | None:intergenic | 55.0% | |
| GGCCGCAATTGGATCTCTTG+CGG | - | chr4.4:66832368-66832387 | None:intergenic | 55.0% | |
| GGGAAAGCCTGTGGGATTTG+TGG | + | chr4.4:66832072-66832091 | MS.gene72550:CDS | 55.0% | |
| TTCGGGAAGGCAAGTCTGTG+AGG | - | chr4.4:66832241-66832260 | None:intergenic | 55.0% | |
| !! | CACAAGCAGACTCTGAAGCC+AGG | + | chr4.4:66832544-66832563 | MS.gene72550:CDS | 55.0% |
| AAGTCTGCTCAGGCACTCGG+TGG | + | chr4.4:66832705-66832724 | MS.gene72550:CDS | 60.0% | |
| GTGGGAAGTGGTTCTCCGGT+GGG | + | chr4.4:66832091-66832110 | MS.gene72550:CDS | 60.0% | |
| TGTGGGAAGTGGTTCTCCGG+TGG | + | chr4.4:66832090-66832109 | MS.gene72550:CDS | 60.0% | |
| TTCTCCGGTGGGAAAGCCCT+TGG | + | chr4.4:66832102-66832121 | MS.gene72550:CDS | 60.0% | |
| ! | AGGCACTCGGTGGACACAAG+AGG | + | chr4.4:66832715-66832734 | MS.gene72550:CDS | 60.0% |
| ! | GGCACTCGGTGGACACAAGA+GGG | + | chr4.4:66832716-66832735 | MS.gene72550:CDS | 60.0% |
| !!! | GCGGGTTGGGTTTTTCGGGT+TGG | - | chr4.4:66832349-66832368 | None:intergenic | 60.0% |
| GCCTCCAAGGGCTTTCCCAC+CGG | - | chr4.4:66832109-66832128 | None:intergenic | 65.0% | |
| TCCGGTGGGAAAGCCCTTGG+AGG | + | chr4.4:66832105-66832124 | MS.gene72550:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 66832057 | 66832788 | 66832057 | ID=MS.gene72550 |
| chr4.4 | mRNA | 66832057 | 66832788 | 66832057 | ID=MS.gene72550.t1;Parent=MS.gene72550 |
| chr4.4 | exon | 66832057 | 66832788 | 66832057 | ID=MS.gene72550.t1.exon1;Parent=MS.gene72550.t1 |
| chr4.4 | CDS | 66832057 | 66832788 | 66832057 | ID=cds.MS.gene72550.t1;Parent=MS.gene72550.t1 |
| Gene Sequence |
| Protein sequence |
