Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72551.t1 | XP_024636235.1 | 94.6 | 241 | 13 | 0 | 1 | 241 | 1 | 241 | 5.50E-110 | 407.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72551.t1 | Q9M202 | 27.8 | 194 | 133 | 4 | 9 | 196 | 6 | 198 | 1.6e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72551.t1 | A0A396I2K7 | 66.9 | 245 | 75 | 1 | 1 | 239 | 1 | 245 | 7.1e-67 | 263.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene72551.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72551.t1 | MTR_4g036615 | 77.885 | 104 | 23 | 0 | 78 | 181 | 1 | 104 | 7.80e-55 | 172 |
| MS.gene72551.t1 | MTR_3g408380 | 28.302 | 265 | 146 | 7 | 3 | 229 | 2 | 260 | 1.30e-21 | 90.9 |
| MS.gene72551.t1 | MTR_3g408420 | 26.165 | 279 | 138 | 7 | 7 | 232 | 6 | 269 | 4.04e-18 | 81.3 |
| MS.gene72551.t1 | MTR_3g008310 | 26.415 | 265 | 157 | 7 | 3 | 229 | 2 | 266 | 4.67e-16 | 75.9 |
| MS.gene72551.t1 | MTR_4g100860 | 59.322 | 59 | 22 | 1 | 173 | 229 | 210 | 268 | 3.10e-13 | 68.2 |
| MS.gene72551.t1 | MTR_0160s0030 | 40.741 | 81 | 38 | 1 | 170 | 240 | 239 | 319 | 8.32e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72551.t1 | AT3G60580 | 30.400 | 250 | 136 | 7 | 9 | 229 | 6 | 246 | 5.55e-18 | 81.3 |
| MS.gene72551.t1 | AT1G02030 | 26.744 | 258 | 133 | 6 | 9 | 236 | 7 | 238 | 8.28e-13 | 66.6 |
| MS.gene72551.t1 | AT5G61470 | 41.096 | 73 | 31 | 2 | 168 | 229 | 225 | 296 | 3.41e-12 | 65.1 |
Find 64 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGCAATTGGATCTCTTGC+GGG | 0.303465 | 4.4:-66769901 | None:intergenic |
| AATTGGATCTCTTGCGGGTT+GGG | 0.313326 | 4.4:-66769896 | None:intergenic |
| ATCCAGGTGTTCTGATGTTA+TGG | 0.324938 | 4.4:+66769967 | MS.gene72551:CDS |
| TGAGTATTAGTTTCAAGCTT+TGG | 0.337317 | 4.4:-66769702 | None:intergenic |
| CAGCCGCAGCATTAACTTCT+CGG | 0.371873 | 4.4:-66769923 | None:intergenic |
| CAATTGGATCTCTTGCGGGT+TGG | 0.402481 | 4.4:-66769897 | None:intergenic |
| ATTAACTTCTCGGCCGCAAT+TGG | 0.433182 | 4.4:-66769913 | None:intergenic |
| TACTCAAGCTGTAGACAATT+CGG | 0.436960 | 4.4:+66769719 | MS.gene72551:CDS |
| ATTTGTGGGAAGTGGTTCTC+CGG | 0.453707 | 4.4:+66769624 | MS.gene72551:CDS |
| GTTTCAAGCTTTGGAGCAAT+CGG | 0.454553 | 4.4:-66769693 | None:intergenic |
| TTCCGATCTTATCAGGCTCT+TGG | 0.457389 | 4.4:+66770131 | MS.gene72551:CDS |
| TTTACAAAGATGCGCAGAAA+AGG | 0.459521 | 4.4:+66769994 | MS.gene72551:CDS |
| TTCTCCGGTGGGAAAGCCCT+TGG | 0.463478 | 4.4:+66769639 | MS.gene72551:CDS |
| GGAAAGCCTGTGGGATTTGT+GGG | 0.467351 | 4.4:+66769610 | MS.gene72551:CDS |
| GGGAAAGCCTGTGGGATTTG+TGG | 0.468727 | 4.4:+66769609 | MS.gene72551:CDS |
| CGATCTTATCAGGCTCTTGG+TGG | 0.468794 | 4.4:+66770134 | MS.gene72551:CDS |
| TTGCAATTTGAGCAGTGAAA+AGG | 0.471865 | 4.4:-66770209 | None:intergenic |
| GCCTCCAAGGGCTTTCCCAC+CGG | 0.472807 | 4.4:-66769643 | None:intergenic |
| CCCCATAACATCAGAACACC+TGG | 0.474579 | 4.4:-66769969 | None:intergenic |
| AGATCTCATGTGGCCTCCAA+GGG | 0.485485 | 4.4:-66769655 | None:intergenic |
| GGCCGCAATTGGATCTCTTG+CGG | 0.489489 | 4.4:-66769902 | None:intergenic |
| TCCAGGTGTTCTGATGTTAT+GGG | 0.494593 | 4.4:+66769968 | MS.gene72551:CDS |
| TCCGGTGGGAAAGCCCTTGG+AGG | 0.494800 | 4.4:+66769642 | MS.gene72551:CDS |
| TTCTCTGTTTGAGCTTGCAG+AGG | 0.496954 | 4.4:-66769847 | None:intergenic |
| TCTGTTTGAGCTTGCAGAGG+AGG | 0.498454 | 4.4:-66769844 | None:intergenic |
| TTACAAAGATGCGCAGAAAA+GGG | 0.499625 | 4.4:+66769995 | MS.gene72551:CDS |
| TTCAAGTCTGCTCAGGCACT+CGG | 0.500253 | 4.4:+66770239 | MS.gene72551:CDS |
| AACGATGGCGAAAGCAGTAC+AGG | 0.507873 | 4.4:+66770047 | MS.gene72551:CDS |
| AGCAAATTGATTTCGACGAT+GGG | 0.509489 | 4.4:-66769751 | None:intergenic |
| GCACCTCTCACAGTTGAACC+TGG | 0.509618 | 4.4:-66770099 | None:intergenic |
| ATTTCGACGATGGGTTGAAT+CGG | 0.510947 | 4.4:-66769742 | None:intergenic |
| GAGATCTCATGTGGCCTCCA+AGG | 0.514030 | 4.4:-66769656 | None:intergenic |
| GAAGATATGTCAAGAGATCC+AGG | 0.516117 | 4.4:+66769951 | MS.gene72551:CDS |
| TGTGGGAAGTGGTTCTCCGG+TGG | 0.517822 | 4.4:+66769627 | MS.gene72551:CDS |
| ACTTTGAAATTCCTTAGCAC+TGG | 0.521740 | 4.4:-66770285 | None:intergenic |
| CAAAGTGTTCAAGTCTGCTC+AGG | 0.524975 | 4.4:+66770232 | MS.gene72551:CDS |
| CACAAGCAGACTCTGAAGCC+AGG | 0.539423 | 4.4:+66770081 | MS.gene72551:CDS |
| AAGCAAATTGATTTCGACGA+TGG | 0.551421 | 4.4:-66769752 | None:intergenic |
| GTGTTTGTTGTATTTCGGGA+AGG | 0.552910 | 4.4:-66769788 | None:intergenic |
| CCTGTGGGATTTGTGGGAAG+TGG | 0.558125 | 4.4:+66769616 | MS.gene72551:CDS |
| GTGGGAAGTGGTTCTCCGGT+GGG | 0.561353 | 4.4:+66769628 | MS.gene72551:CDS |
| GATCTTATCAGGCTCTTGGT+GGG | 0.571526 | 4.4:+66770135 | MS.gene72551:CDS |
| GGTACACTCTTCCAGTGCTA+AGG | 0.575791 | 4.4:+66770274 | MS.gene72551:CDS |
| GCAACTATGGAGAAGAGCGA+TGG | 0.579019 | 4.4:+66769588 | None:intergenic |
| AAAGATGCGCAGAAAAGGGA+AGG | 0.579501 | 4.4:+66769999 | MS.gene72551:CDS |
| CACCAAGAGCCTGATAAGAT+CGG | 0.582662 | 4.4:-66770133 | None:intergenic |
| CCAGGTGTTCTGATGTTATG+GGG | 0.595199 | 4.4:+66769969 | MS.gene72551:CDS |
| AAGAGCGATGGGAAAGCCTG+TGG | 0.598960 | 4.4:+66769600 | MS.gene72551:CDS |
| AGGCACTCGGTGGACACAAG+AGG | 0.606213 | 4.4:+66770252 | MS.gene72551:CDS |
| CAACTATGGAGAAGAGCGAT+GGG | 0.616240 | 4.4:+66769589 | None:intergenic |
| TACTGCAAAAGAGACAATGA+TGG | 0.621835 | 4.4:+66770167 | MS.gene72551:CDS |
| AAGCCAGGTTCAACTGTGAG+AGG | 0.622627 | 4.4:+66770096 | MS.gene72551:CDS |
| ATCACAAATCAATACGGGTG+TGG | 0.631203 | 4.4:-66770311 | None:intergenic |
| GGCACTCGGTGGACACAAGA+GGG | 0.632635 | 4.4:+66770253 | MS.gene72551:CDS |
| CCACTTCCCACAAATCCCAC+AGG | 0.647858 | 4.4:-66769616 | None:intergenic |
| CGGCCGAGAAGTTAATGCTG+CGG | 0.653910 | 4.4:+66769920 | MS.gene72551:CDS |
| AGAGCGATGGGAAAGCCTGT+GGG | 0.654994 | 4.4:+66769601 | MS.gene72551:CDS |
| AGGTTCAACTGTGAGAGGTG+CGG | 0.663022 | 4.4:+66770101 | MS.gene72551:CDS |
| AAGATGCGCAGAAAAGGGAA+GGG | 0.667779 | 4.4:+66770000 | MS.gene72551:CDS |
| TTCGGGAAGGCAAGTCTGTG+AGG | 0.669270 | 4.4:-66769775 | None:intergenic |
| AAGTCTGCTCAGGCACTCGG+TGG | 0.670340 | 4.4:+66770242 | MS.gene72551:CDS |
| AGGCCACATGAGATCTCACA+TGG | 0.682156 | 4.4:+66769662 | MS.gene72551:CDS |
| ACCCGCAAGAGATCCAATTG+CGG | 0.686880 | 4.4:+66769900 | MS.gene72551:CDS |
| GAACCATGTGAGATCTCATG+TGG | 0.730676 | 4.4:-66769665 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGTGTTTGTTGTATTT+CGG | - | chr4.4:66769796-66769815 | None:intergenic | 20.0% |
| ! | GATCAAGAAACAAAAAATGA+GGG | + | chr4.4:66770022-66770041 | MS.gene72551:CDS | 25.0% |
| !!! | TTTTGTGTTTGTTGTATTTC+GGG | - | chr4.4:66769795-66769814 | None:intergenic | 25.0% |
| AAAAATGTTCCGATCTTATC+AGG | + | chr4.4:66770124-66770143 | MS.gene72551:CDS | 30.0% | |
| GGATCAAGAAACAAAAAATG+AGG | + | chr4.4:66770021-66770040 | MS.gene72551:CDS | 30.0% | |
| ! | TGAGTATTAGTTTCAAGCTT+TGG | - | chr4.4:66769705-66769724 | None:intergenic | 30.0% |
| !!! | TGAAGTTTTGTTTCAAGGAT+AGG | - | chr4.4:66769821-66769840 | None:intergenic | 30.0% |
| CAAAAAATGAGGGAAAACGA+TGG | + | chr4.4:66770032-66770051 | MS.gene72551:CDS | 35.0% | |
| TACTCAAGCTGTAGACAATT+CGG | + | chr4.4:66769719-66769738 | MS.gene72551:CDS | 35.0% | |
| TACTGCAAAAGAGACAATGA+TGG | + | chr4.4:66770167-66770186 | MS.gene72551:CDS | 35.0% | |
| TTACAAAGATGCGCAGAAAA+GGG | + | chr4.4:66769995-66770014 | MS.gene72551:CDS | 35.0% | |
| TTGCAATTTGAGCAGTGAAA+AGG | - | chr4.4:66770212-66770231 | None:intergenic | 35.0% | |
| TTTACAAAGATGCGCAGAAA+AGG | + | chr4.4:66769994-66770013 | MS.gene72551:CDS | 35.0% | |
| ! | AAGCAAATTGATTTCGACGA+TGG | - | chr4.4:66769755-66769774 | None:intergenic | 35.0% |
| ! | AGCAAATTGATTTCGACGAT+GGG | - | chr4.4:66769754-66769773 | None:intergenic | 35.0% |
| !! | ACTTTGAAATTCCTTAGCAC+TGG | - | chr4.4:66770288-66770307 | None:intergenic | 35.0% |
| ATCCAGGTGTTCTGATGTTA+TGG | + | chr4.4:66769967-66769986 | MS.gene72551:CDS | 40.0% | |
| GAAGATATGTCAAGAGATCC+AGG | + | chr4.4:66769951-66769970 | MS.gene72551:CDS | 40.0% | |
| TCCAGGTGTTCTGATGTTAT+GGG | + | chr4.4:66769968-66769987 | MS.gene72551:CDS | 40.0% | |
| !! | ATTTCGACGATGGGTTGAAT+CGG | - | chr4.4:66769745-66769764 | None:intergenic | 40.0% |
| !! | GTGTTTGTTGTATTTCGGGA+AGG | - | chr4.4:66769791-66769810 | None:intergenic | 40.0% |
| !! | GTTTCAAGCTTTGGAGCAAT+CGG | - | chr4.4:66769696-66769715 | None:intergenic | 40.0% |
| !!! | GGCAGTGAAGTTTTGTTTCA+AGG | - | chr4.4:66769826-66769845 | None:intergenic | 40.0% |
| AAAGATGCGCAGAAAAGGGA+AGG | + | chr4.4:66769999-66770018 | MS.gene72551:CDS | 45.0% | |
| AAGATGCGCAGAAAAGGGAA+GGG | + | chr4.4:66770000-66770019 | MS.gene72551:CDS | 45.0% | |
| AATTGGATCTCTTGCGGGTT+GGG | - | chr4.4:66769899-66769918 | None:intergenic | 45.0% | |
| ATTAACTTCTCGGCCGCAAT+TGG | - | chr4.4:66769916-66769935 | None:intergenic | 45.0% | |
| ATTTGTGGGAAGTGGTTCTC+CGG | + | chr4.4:66769624-66769643 | MS.gene72551:CDS | 45.0% | |
| CACCAAGAGCCTGATAAGAT+CGG | - | chr4.4:66770136-66770155 | None:intergenic | 45.0% | |
| CCAGGTGTTCTGATGTTATG+GGG | + | chr4.4:66769969-66769988 | MS.gene72551:CDS | 45.0% | |
| GAACCATGTGAGATCTCATG+TGG | - | chr4.4:66769668-66769687 | None:intergenic | 45.0% | |
| GATCTTATCAGGCTCTTGGT+GGG | + | chr4.4:66770135-66770154 | MS.gene72551:CDS | 45.0% | |
| TTCCGATCTTATCAGGCTCT+TGG | + | chr4.4:66770131-66770150 | MS.gene72551:CDS | 45.0% | |
| TTCTCTGTTTGAGCTTGCAG+AGG | - | chr4.4:66769850-66769869 | None:intergenic | 45.0% | |
| ! | CAAAGTGTTCAAGTCTGCTC+AGG | + | chr4.4:66770232-66770251 | MS.gene72551:CDS | 45.0% |
| AACGATGGCGAAAGCAGTAC+AGG | + | chr4.4:66770047-66770066 | MS.gene72551:CDS | 50.0% | |
| AAGCCAGGTTCAACTGTGAG+AGG | + | chr4.4:66770096-66770115 | MS.gene72551:CDS | 50.0% | |
| ACCCGCAAGAGATCCAATTG+CGG | + | chr4.4:66769900-66769919 | MS.gene72551:CDS | 50.0% | |
| AGATCTCATGTGGCCTCCAA+GGG | - | chr4.4:66769658-66769677 | None:intergenic | 50.0% | |
| AGGCCACATGAGATCTCACA+TGG | + | chr4.4:66769662-66769681 | MS.gene72551:CDS | 50.0% | |
| AGGTTCAACTGTGAGAGGTG+CGG | + | chr4.4:66770101-66770120 | MS.gene72551:CDS | 50.0% | |
| CAATTGGATCTCTTGCGGGT+TGG | - | chr4.4:66769900-66769919 | None:intergenic | 50.0% | |
| CAGCCGCAGCATTAACTTCT+CGG | - | chr4.4:66769926-66769945 | None:intergenic | 50.0% | |
| CCCCATAACATCAGAACACC+TGG | - | chr4.4:66769972-66769991 | None:intergenic | 50.0% | |
| CGATCTTATCAGGCTCTTGG+TGG | + | chr4.4:66770134-66770153 | MS.gene72551:CDS | 50.0% | |
| GGAAAGCCTGTGGGATTTGT+GGG | + | chr4.4:66769610-66769629 | MS.gene72551:CDS | 50.0% | |
| GGTACACTCTTCCAGTGCTA+AGG | + | chr4.4:66770274-66770293 | MS.gene72551:CDS | 50.0% | |
| TCTGTTTGAGCTTGCAGAGG+AGG | - | chr4.4:66769847-66769866 | None:intergenic | 50.0% | |
| TTCAAGTCTGCTCAGGCACT+CGG | + | chr4.4:66770239-66770258 | MS.gene72551:CDS | 50.0% | |
| TTCCCATCGCTCTTCTCCAT+GGG | - | chr4.4:66769594-66769613 | None:intergenic | 50.0% | |
| TTTCCCATCGCTCTTCTCCA+TGG | - | chr4.4:66769595-66769614 | None:intergenic | 50.0% | |
| !!! | CTCTTGCGGGTTGGGTTTTT+CGG | - | chr4.4:66769891-66769910 | None:intergenic | 50.0% |
| !!! | TCTTGCGGGTTGGGTTTTTC+GGG | - | chr4.4:66769890-66769909 | None:intergenic | 50.0% |
| AAGAGCGATGGGAAAGCCTG+TGG | + | chr4.4:66769600-66769619 | MS.gene72551:CDS | 55.0% | |
| AGAGCGATGGGAAAGCCTGT+GGG | + | chr4.4:66769601-66769620 | MS.gene72551:CDS | 55.0% | |
| CCACTTCCCACAAATCCCAC+AGG | - | chr4.4:66769619-66769638 | None:intergenic | 55.0% | |
| CCTGTGGGATTTGTGGGAAG+TGG | + | chr4.4:66769616-66769635 | MS.gene72551:CDS | 55.0% | |
| CGGCCGAGAAGTTAATGCTG+CGG | + | chr4.4:66769920-66769939 | MS.gene72551:CDS | 55.0% | |
| GAGATCTCATGTGGCCTCCA+AGG | - | chr4.4:66769659-66769678 | None:intergenic | 55.0% | |
| GCACCTCTCACAGTTGAACC+TGG | - | chr4.4:66770102-66770121 | None:intergenic | 55.0% | |
| GCCGCAATTGGATCTCTTGC+GGG | - | chr4.4:66769904-66769923 | None:intergenic | 55.0% | |
| GGCCGCAATTGGATCTCTTG+CGG | - | chr4.4:66769905-66769924 | None:intergenic | 55.0% | |
| GGGAAAGCCTGTGGGATTTG+TGG | + | chr4.4:66769609-66769628 | MS.gene72551:CDS | 55.0% | |
| TTCGGGAAGGCAAGTCTGTG+AGG | - | chr4.4:66769778-66769797 | None:intergenic | 55.0% | |
| !! | CACAAGCAGACTCTGAAGCC+AGG | + | chr4.4:66770081-66770100 | MS.gene72551:CDS | 55.0% |
| AAGTCTGCTCAGGCACTCGG+TGG | + | chr4.4:66770242-66770261 | MS.gene72551:CDS | 60.0% | |
| GTGGGAAGTGGTTCTCCGGT+GGG | + | chr4.4:66769628-66769647 | MS.gene72551:CDS | 60.0% | |
| TGTGGGAAGTGGTTCTCCGG+TGG | + | chr4.4:66769627-66769646 | MS.gene72551:CDS | 60.0% | |
| TTCTCCGGTGGGAAAGCCCT+TGG | + | chr4.4:66769639-66769658 | MS.gene72551:CDS | 60.0% | |
| ! | AGGCACTCGGTGGACACAAG+AGG | + | chr4.4:66770252-66770271 | MS.gene72551:CDS | 60.0% |
| ! | GGCACTCGGTGGACACAAGA+GGG | + | chr4.4:66770253-66770272 | MS.gene72551:CDS | 60.0% |
| !!! | GCGGGTTGGGTTTTTCGGGT+TGG | - | chr4.4:66769886-66769905 | None:intergenic | 60.0% |
| GCCTCCAAGGGCTTTCCCAC+CGG | - | chr4.4:66769646-66769665 | None:intergenic | 65.0% | |
| TCCGGTGGGAAAGCCCTTGG+AGG | + | chr4.4:66769642-66769661 | MS.gene72551:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 66769594 | 66770318 | 66769594 | ID=MS.gene72551 |
| chr4.4 | mRNA | 66769594 | 66770318 | 66769594 | ID=MS.gene72551.t1;Parent=MS.gene72551 |
| chr4.4 | exon | 66769594 | 66770318 | 66769594 | ID=MS.gene72551.t1.exon1;Parent=MS.gene72551.t1 |
| chr4.4 | CDS | 66769594 | 66770318 | 66769594 | ID=cds.MS.gene72551.t1;Parent=MS.gene72551.t1 |
| Gene Sequence |
| Protein sequence |
