AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72850.t1 MTR_5g027030 89.728 331 26 2 1 330 1 324 0.0 577
MS.gene72850.t1 MTR_0001s0490 47.706 218 64 9 16 212 25 213 4.95e-49 167
MS.gene72850.t1 MTR_2g041430 60.177 113 29 2 16 112 7 119 2.04e-40 145
MS.gene72850.t1 MTR_2g041430 60.177 113 29 2 16 112 7 119 2.06e-40 145
MS.gene72850.t1 MTR_2g041430 60.177 113 29 2 16 112 7 119 2.31e-40 145
MS.gene72850.t1 MTR_4g125490 34.643 280 132 8 16 256 9 276 2.78e-40 146
MS.gene72850.t1 MTR_8g020560 58.333 108 33 2 17 112 12 119 3.98e-39 141
MS.gene72850.t1 MTR_1g017490 56.897 116 34 2 16 115 11 126 7.92e-37 139
MS.gene72850.t1 MTR_1g017490 56.897 116 34 2 16 115 144 259 1.24e-36 139
MS.gene72850.t1 MTR_7g104050 62.037 108 29 4 17 112 79 186 1.44e-35 133
MS.gene72850.t1 MTR_7g104050 59.649 114 34 4 17 118 79 192 1.61e-35 132
MS.gene72850.t1 MTR_3g092330 53.000 100 39 2 14 112 111 203 7.23e-27 110
MS.gene72850.t1 MTR_8g035910 45.794 107 42 3 15 114 48 145 1.25e-19 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72850.t1 AT3G13960 57.273 110 35 2 15 112 15 124 1.29e-38 141
MS.gene72850.t1 AT4G37740 48.649 148 62 3 16 153 164 307 1.07e-37 141
MS.gene72850.t1 AT3G52910 56.250 112 33 3 17 112 83 194 2.49e-33 127
MS.gene72850.t1 AT2G36400 54.783 115 37 3 17 116 77 191 4.02e-33 126
MS.gene72850.t1 AT2G22840 54.128 109 36 2 16 112 133 239 7.03e-31 122
MS.gene72850.t1 AT2G06200 50.400 125 39 3 9 110 38 162 1.67e-30 117
MS.gene72850.t1 AT2G06200 52.066 121 35 3 13 110 3 123 2.72e-30 115
MS.gene72850.t1 AT4G24150 46.610 118 47 2 17 118 112 229 3.79e-24 102
MS.gene72850.t1 AT4G24150 46.610 118 47 2 17 118 150 267 8.47e-24 101
MS.gene72850.t1 AT5G53660 49.485 97 44 1 16 112 59 150 1.08e-22 97.4
MS.gene72850.t1 AT4G24150 37.762 143 48 1 17 118 150 292 1.98e-21 95.1
MS.gene72850.t1 AT2G45480 39.815 108 51 2 15 109 23 129 6.09e-17 80.5
MS.gene72850.t1 AT2G06200 72.917 48 11 1 65 110 4 51 6.26e-17 77.8
MS.gene72850.t1 AT2G45480 39.815 108 51 2 15 109 21 127 1.84e-16 80.1
MS.gene72850.t1 AT2G45480 36.458 96 48 3 21 116 269 351 1.11e-12 68.9
MS.gene72850.t1 AT2G45480 39.815 108 51 2 15 109 23 129 1.89e-16 80.1
MS.gene72850.t1 AT2G45480 36.458 96 48 3 21 116 271 353 1.16e-12 68.9

Find 77 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATGCTTCTTCCTCTTC+AGG 0.174652 5.1:-17674687 MS.gene72850:CDS
CTTCTACACCTACCTGGTTC+TGG 0.179105 5.1:+17675034 None:intergenic
GAAGGTTTAAGTTTCAATTT+TGG 0.205836 5.1:-17674731 MS.gene72850:CDS
TTTGTTTCAGTGGGTTATTA+TGG 0.331715 5.1:-17675070 MS.gene72850:intron
TTCTACACCTACCTGGTTCT+GGG 0.343240 5.1:+17675035 None:intergenic
TTCCTAGATCCTTCTGTAAG+AGG 0.359958 5.1:+17672808 None:intergenic
CCTATGCTTCTTCCTCTTCA+GGG 0.369011 5.1:-17674686 MS.gene72850:CDS
GATGTTCTAGCTCTTGCCAT+TGG 0.387285 5.1:+17675271 None:intergenic
TTTCAATCAACATTGACATC+TGG 0.399287 5.1:+17672465 None:intergenic
CCTGTTTCAAGCTACCTAAC+TGG 0.407084 5.1:+17672589 None:intergenic
CAGGTTCTCTTGTTCGCTGT+TGG 0.415451 5.1:+17672661 None:intergenic
ATGTTCTAGCTCTTGCCATT+GGG 0.417081 5.1:+17675272 None:intergenic
CTCTCTTCAGTGACTGGCAG+TGG 0.424103 5.1:-17672548 MS.gene72850:CDS
ATCGTCTTTAACGGCCAGTT+AGG 0.430343 5.1:-17672603 MS.gene72850:CDS
CCAGTTAGGTAGCTTGAAAC+AGG 0.432238 5.1:-17672589 MS.gene72850:CDS
AGGGTTCTAAGAAACCTTAT+AGG 0.432493 5.1:-17674667 MS.gene72850:intron
CTCAGTCTCTCTTCAGTGAC+TGG 0.441726 5.1:-17672554 MS.gene72850:CDS
ACACTTCCTTTGCATACAAA+TGG 0.444626 5.1:-17674761 MS.gene72850:CDS
TCCAAGGAAATCGTCTTTAA+CGG 0.448844 5.1:-17672612 MS.gene72850:CDS
CTTGGTGCTGGTGGTTCTAG+TGG 0.451681 5.1:-17674830 MS.gene72850:CDS
CGGTCACAGTACTTAGAATC+AGG 0.455848 5.1:+17674971 None:intergenic
GCCGTTAAAGACGATTTCCT+TGG 0.472384 5.1:+17672611 None:intergenic
CAAACACTGTTTCAGAATCA+AGG 0.474671 5.1:-17672716 MS.gene72850:CDS
ATCTTCAAGTATCTGAAAGC+TGG 0.478992 5.1:-17675240 MS.gene72850:CDS
CCTCCTCTGCAATGGAGTAC+AGG 0.482812 5.1:-17672512 MS.gene72850:CDS
CGAATAGGTAAAAGGAGGTC+AGG 0.485852 5.1:+17675204 None:intergenic
CATAGGGTCCATGTGGGGTA+TGG 0.486854 5.1:+17674703 None:intergenic
TTGGTGTTAAAATCCTTGCT+TGG 0.489894 5.1:+17672489 None:intergenic
ATCAACATTGACATCTGGAT+TGG 0.498766 5.1:+17672470 None:intergenic
AACCTCTTACAGAAGGATCT+AGG 0.500686 5.1:-17672810 MS.gene72850:CDS
CTAGGAACTGTGAAGTATCA+AGG 0.513671 5.1:-17672792 MS.gene72850:CDS
ATTCCACAGGCTTTCTTGAA+CGG 0.519412 5.1:+17674927 None:intergenic
CCCTGAAGAGGAAGAAGCAT+AGG 0.524973 5.1:+17674686 None:intergenic
GCGTCGAGGTTAAACAGCTC+AGG 0.529759 5.1:+17672642 None:intergenic
ACTTCACAGCAACTTGGTGC+TGG 0.533655 5.1:-17674842 MS.gene72850:CDS
CATACAAATGGTACTCGTGA+AGG 0.534280 5.1:-17674749 MS.gene72850:CDS
GGTTATGACTTCACTTCAGA+TGG 0.536609 5.1:-17672771 MS.gene72850:CDS
CCTGTACTCCATTGCAGAGG+AGG 0.538667 5.1:+17672512 None:intergenic
TCTGTTCTTCTACACCTACC+TGG 0.541473 5.1:+17675028 None:intergenic
CGAGTACCATTTGTATGCAA+AGG 0.542113 5.1:+17674755 None:intergenic
TCACAGCAACTTGGTGCTGG+TGG 0.545124 5.1:-17674839 MS.gene72850:CDS
TAATAGGTTTGGACTGAACT+CGG 0.548879 5.1:-17672847 MS.gene72850:intron
AGTACTACTTCACAGCAACT+TGG 0.553505 5.1:-17674848 MS.gene72850:CDS
AGATTTAGCCTCCTCTGCAA+TGG 0.559132 5.1:-17672520 MS.gene72850:CDS
TTCAACAACCGCCGTTGATG+TGG 0.565403 5.1:-17675323 MS.gene72850:CDS
TGGGAACACCATACCCCACA+TGG 0.566300 5.1:-17674711 MS.gene72850:CDS
TGCCATTGGGAGACTGTGAA+CGG 0.571539 5.1:+17675285 None:intergenic
TGTTTAACCTCGACGCTGCA+AGG 0.571608 5.1:-17672635 MS.gene72850:CDS
CCTCGACGCTGCAAGGTCCA+AGG 0.583292 5.1:-17672628 MS.gene72850:CDS
ACTGTTTCAGAATCAAGGAA+TGG 0.590210 5.1:-17672711 MS.gene72850:CDS
GAAGAAGAAGAAGATTCCAC+AGG 0.592735 5.1:+17674914 None:intergenic
CCTTGGACCTTGCAGCGTCG+AGG 0.593781 5.1:+17672628 None:intergenic
TGGACTGAACTCGGAAGCAG+AGG 0.606816 5.1:-17672838 MS.gene72850:CDS
AGTCACTGAAGAGAGACTGA+GGG 0.611044 5.1:+17672556 None:intergenic
CACCGTTCACAGTCTCCCAA+TGG 0.611493 5.1:-17675287 MS.gene72850:CDS
GGAGAACAACCTCTTACAGA+AGG 0.617644 5.1:-17672817 MS.gene72850:CDS
ACTGTGAACGGTGGATGAGT+TGG 0.621139 5.1:+17675297 None:intergenic
AATGGAGTACAGGCCAAGCA+AGG 0.625052 5.1:-17672502 MS.gene72850:CDS
GGTAGGTGTAGAAGAACAGA+TGG 0.627953 5.1:-17675025 MS.gene72850:CDS
CAGTCACTGAAGAGAGACTG+AGG 0.628605 5.1:+17672555 None:intergenic
GGTTTCTTAGAACCCTGAAG+AGG 0.629209 5.1:+17674674 None:intergenic
CCTGAAGAGGAAGAAGCATA+GGG 0.630158 5.1:+17674687 None:intergenic
AAGAAGCATAGGGTCCATGT+GGG 0.631241 5.1:+17674697 None:intergenic
GAAGAAGCATAGGGTCCATG+TGG 0.633928 5.1:+17674696 None:intergenic
TGAGTTGGTGACCACATCAA+CGG 0.634344 5.1:+17675312 None:intergenic
TGGCCTGTACTCCATTGCAG+AGG 0.635477 5.1:+17672509 None:intergenic
ATCAAGGAATGGTTCTACCA+TGG 0.655574 5.1:-17672700 MS.gene72850:CDS
ATACCGTTCAAGAAAGCCTG+TGG 0.663730 5.1:-17674930 MS.gene72850:CDS
ACTTCACTTCAGATGGCATG+TGG 0.666198 5.1:-17672764 MS.gene72850:CDS
AAATTGACCCAGAACCAGGT+AGG 0.671324 5.1:-17675042 MS.gene72850:CDS
GAAGTTGTAGTTGTCAACCA+TGG 0.681166 5.1:+17672683 None:intergenic
GTCACTGAAGAGAGACTGAG+GGG 0.689928 5.1:+17672557 None:intergenic
ATCGACGTCGAATCATATGA+CGG 0.690045 5.1:+17674951 None:intergenic
ATAGGTAAAAGGAGGTCAGG+AGG 0.695933 5.1:+17675207 None:intergenic
AGAAGCATAGGGTCCATGTG+GGG 0.729236 5.1:+17674698 None:intergenic
CATTGGGAGACTGTGAACGG+TGG 0.744499 5.1:+17675288 None:intergenic
GTTGGTGACCACATCAACGG+CGG 0.749963 5.1:+17675315 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTCTTTTTATATCTATT+TGG - chr5.1:17674349-17674368 MS.gene72850:intron 10.0%
!!! TTTTATATCTTTATGTTAAA+TGG - chr5.1:17673548-17673567 MS.gene72850:intron 10.0%
!!! TTTTTTTTTTGTTTTCAATA+TGG - chr5.1:17673181-17673200 MS.gene72850:intron 10.0%
!!! AAGTGATTTTACTTTTTATA+AGG - chr5.1:17674721-17674740 MS.gene72850:CDS 15.0%
!!! ATACTTTATTTTATTCAACT+TGG - chr5.1:17673720-17673739 MS.gene72850:intron 15.0%
!!! GGATATTAATATTTTTATGA+AGG - chr5.1:17674769-17674788 MS.gene72850:CDS 15.0%
!!! TATTAATATTTTTATGAAGG+TGG - chr5.1:17674772-17674791 MS.gene72850:CDS 15.0%
!! AAAAAAGAAAAGAAGAGAAA+TGG - chr5.1:17674656-17674675 MS.gene72850:intron 20.0%
!! AAAAAAGAAACATACCTATA+AGG + chr5.1:17673147-17673166 None:intergenic 20.0%
!! AATCAATTGTGTAAACAAAA+AGG + chr5.1:17673530-17673549 None:intergenic 20.0%
!! ATCAAAATAGAGACAAAATA+AGG + chr5.1:17672676-17672695 None:intergenic 20.0%
!! TTTATGTTAAATGGAGAATA+TGG - chr5.1:17673557-17673576 MS.gene72850:intron 20.0%
!!! AAGGTTTAAGTTTCAATTTT+GGG - chr5.1:17673067-17673086 MS.gene72850:intron 20.0%
!!! ACTATATTTTGTTTTGACAA+AGG - chr5.1:17673694-17673713 MS.gene72850:intron 20.0%
!!! CGATATGATTTTTTAATTCT+AGG - chr5.1:17674372-17674391 MS.gene72850:intron 20.0%
! ATCCTGAATTGAATAATTCT+TGG + chr5.1:17673296-17673315 None:intergenic 25.0%
! ATTTCAAATGACCATCAAAA+AGG + chr5.1:17673894-17673913 None:intergenic 25.0%
! GTGATAATATTGGAAATCAA+GGG - chr5.1:17674023-17674042 MS.gene72850:intron 25.0%
! TAAGTATTATCAGAAGTTGT+TGG - chr5.1:17674863-17674882 MS.gene72850:CDS 25.0%
! TACTTAACACACTCAATAAT+AGG - chr5.1:17674934-17674953 MS.gene72850:CDS 25.0%
! TATTTGAACAAGTAATAACC+TGG + chr5.1:17673931-17673950 None:intergenic 25.0%
! TGTGATAATATTGGAAATCA+AGG - chr5.1:17674022-17674041 MS.gene72850:intron 25.0%
! TTAAGACCAAAAACAAATGT+CGG + chr5.1:17674152-17674171 None:intergenic 25.0%
! TTTGTGCATTGGATTTAATT+AGG - chr5.1:17673810-17673829 MS.gene72850:intron 25.0%
!! ACTTTTTATAAGGAACTTGT+AGG - chr5.1:17674731-17674750 MS.gene72850:CDS 25.0%
!! TTATAGCAAGAGTTGTATTT+TGG - chr5.1:17674687-17674706 MS.gene72850:CDS 25.0%
!!! AATATTTTGAGTGAACTAAG+AGG - chr5.1:17673994-17674013 MS.gene72850:intron 25.0%
!!! ATTTTTATGAAGGTGGATTA+AGG - chr5.1:17674779-17674798 MS.gene72850:CDS 25.0%
!!! GAAGGTTTAAGTTTCAATTT+TGG - chr5.1:17673066-17673085 MS.gene72850:intron 25.0%
!!! GTTTATTCTCTACTTTTAGA+CGG - chr5.1:17674474-17674493 MS.gene72850:intron 25.0%
!!! GTTTGTGTATTTGTTTTTTG+AGG - chr5.1:17673434-17673453 MS.gene72850:intron 25.0%
!!! TTTGTGATTTTTGTTTCAGT+GGG - chr5.1:17672718-17672737 MS.gene72850:CDS 25.0%
!!! TTTTGTGATTTTTGTTTCAG+TGG - chr5.1:17672717-17672736 MS.gene72850:CDS 25.0%
AACACACTCAATAATAGGTT+TGG - chr5.1:17674939-17674958 MS.gene72850:CDS 30.0%
AAGTTACATAACATGTGATG+TGG - chr5.1:17673593-17673612 MS.gene72850:intron 30.0%
ATAAGGAAGATTAACGTTGT+TGG + chr5.1:17672659-17672678 None:intergenic 30.0%
ATATCACGACATCTAATTGA+TGG + chr5.1:17674398-17674417 None:intergenic 30.0%
GAGTTAAAGCAATTAAGTAG+AGG - chr5.1:17674104-17674123 MS.gene72850:intron 30.0%
TAAGGAAGATTAACGTTGTT+GGG + chr5.1:17672658-17672677 None:intergenic 30.0%
! AAACTGAATTGCCTTTTTGA+TGG - chr5.1:17673880-17673899 MS.gene72850:intron 30.0%
! AATTGAAGACTTTTCCGAAT+AGG + chr5.1:17672611-17672630 None:intergenic 30.0%
! ACTTGATGATTATCCAGAAA+GGG - chr5.1:17674261-17674280 MS.gene72850:intron 30.0%
! AGTTAGATGTTGGTTAGTTA+TGG + chr5.1:17674220-17674239 None:intergenic 30.0%
! GGTCATTTGAAATCATGATT+CGG - chr5.1:17673901-17673920 MS.gene72850:intron 30.0%
! TACTTGATGATTATCCAGAA+AGG - chr5.1:17674260-17674279 MS.gene72850:intron 30.0%
! TGGTAAAAGATTTTGTGCAT+TGG - chr5.1:17673799-17673818 MS.gene72850:intron 30.0%
!! TTTGTTTCAGTGGGTTATTA+TGG - chr5.1:17672727-17672746 MS.gene72850:CDS 30.0%
AAAAAAATTGACCCAGAACC+AGG - chr5.1:17672751-17672770 MS.gene72850:CDS 35.0%
AATTGCAAAAGACTACACGT+TGG + chr5.1:17674836-17674855 None:intergenic 35.0%
ACAATGTACATTGCTGAACA+AGG + chr5.1:17674566-17674585 None:intergenic 35.0%
ACACTTCCTTTGCATACAAA+TGG - chr5.1:17673036-17673055 MS.gene72850:intron 35.0%
ACCAATCACTTAATTGGGTT+TGG - chr5.1:17673769-17673788 MS.gene72850:intron 35.0%
ACTGTTTCAGAATCAAGGAA+TGG - chr5.1:17675086-17675105 MS.gene72850:intron 35.0%
AGTCAAACAAGAATGCAAGT+AGG + chr5.1:17674454-17674473 None:intergenic 35.0%
ATCAACATTGACATCTGGAT+TGG + chr5.1:17675330-17675349 None:intergenic 35.0%
ATCTTCAAGTATCTGAAAGC+TGG - chr5.1:17672557-17672576 MS.gene72850:CDS 35.0%
ATTGGAAATCAAGGGAATTG+TGG - chr5.1:17674031-17674050 MS.gene72850:intron 35.0%
CAAAAAATGGAGATGTGCTA+GGG - chr5.1:17672795-17672814 MS.gene72850:CDS 35.0%
CAAACACTGTTTCAGAATCA+AGG - chr5.1:17675081-17675100 MS.gene72850:intron 35.0%
GAAGAACAGATGGCAAAAAA+TGG - chr5.1:17672782-17672801 MS.gene72850:CDS 35.0%
GAGGCATGATGTGATAATAT+TGG - chr5.1:17674013-17674032 MS.gene72850:intron 35.0%
GATGACATGTTGAAGGAATT+TGG - chr5.1:17674318-17674337 MS.gene72850:intron 35.0%
GCAGTACCAATCACTTAATT+GGG - chr5.1:17673764-17673783 MS.gene72850:intron 35.0%
GGCCAAGAATTATTCAATTC+AGG - chr5.1:17673291-17673310 MS.gene72850:intron 35.0%
GTTAGATGCTAGTTAGATGT+TGG + chr5.1:17674230-17674249 None:intergenic 35.0%
GTTTGGAGATAAGTAAAGTC+TGG - chr5.1:17673270-17673289 MS.gene72850:intron 35.0%
TAACACTTCGCAAGAAATCA+TGG + chr5.1:17673494-17673513 None:intergenic 35.0%
TAATAGGTTTGGACTGAACT+CGG - chr5.1:17674950-17674969 MS.gene72850:CDS 35.0%
TACATTGCTGAACAAGGAAA+AGG + chr5.1:17674560-17674579 None:intergenic 35.0%
TCCAAGGAAATCGTCTTTAA+CGG - chr5.1:17675185-17675204 MS.gene72850:CDS 35.0%
TGCAGTACCAATCACTTAAT+TGG - chr5.1:17673763-17673782 MS.gene72850:intron 35.0%
TGTCATTGATACCATGTAGA+TGG - chr5.1:17674583-17674602 MS.gene72850:intron 35.0%
TTGTGTATTCGTGTGTGAAT+GGG - chr5.1:17673362-17673381 MS.gene72850:intron 35.0%
! AAGTCATCTAGCCATAGTTT+TGG - chr5.1:17673660-17673679 MS.gene72850:intron 35.0%
! GACTTTTCCGAATAGGTAAA+AGG + chr5.1:17672604-17672623 None:intergenic 35.0%
! TTTTCCGAATAGGTAAAAGG+AGG + chr5.1:17672601-17672620 None:intergenic 35.0%
!! AAATCATGATTCGGTATTCC+AGG - chr5.1:17673910-17673929 MS.gene72850:intron 35.0%
!! AACCAACCGACATTTGTTTT+TGG - chr5.1:17674143-17674162 MS.gene72850:intron 35.0%
!! AGGGTTCTAAGAAACCTTAT+AGG - chr5.1:17673130-17673149 MS.gene72850:intron 35.0%
!! TTGGTGTTAAAATCCTTGCT+TGG + chr5.1:17675311-17675330 None:intergenic 35.0%
!!! TGTTTTTGGTCTTAATCCTC+TGG - chr5.1:17674157-17674176 MS.gene72850:intron 35.0%
AAACCGAACTCTGGATTATC+AGG + chr5.1:17673254-17673273 None:intergenic 40.0%
AACATGTCATCCCATAACAC+CGG + chr5.1:17674309-17674328 None:intergenic 40.0%
AACCGAACTCTGGATTATCA+GGG + chr5.1:17673253-17673272 None:intergenic 40.0%
AACCTCTTACAGAAGGATCT+AGG - chr5.1:17674987-17675006 MS.gene72850:CDS 40.0%
ACAAGCCAACTCCAAAACTA+TGG + chr5.1:17673674-17673693 None:intergenic 40.0%
AGTACTACTTCACAGCAACT+TGG - chr5.1:17672949-17672968 MS.gene72850:intron 40.0%
ATCCTTATCATGTGTGTGTG+GGG - chr5.1:17673616-17673635 MS.gene72850:intron 40.0%
ATCGACGTCGAATCATATGA+CGG + chr5.1:17672849-17672868 None:intergenic 40.0%
ATTCCACAGGCTTTCTTGAA+CGG + chr5.1:17672873-17672892 None:intergenic 40.0%
CAAACAAGAATGCAAGTAGG+AGG + chr5.1:17674451-17674470 None:intergenic 40.0%
CATACAAATGGTACTCGTGA+AGG - chr5.1:17673048-17673067 MS.gene72850:intron 40.0%
CGAGTACCATTTGTATGCAA+AGG + chr5.1:17673045-17673064 None:intergenic 40.0%
CGTTGGATCATTAAGGCTTA+AGG + chr5.1:17674819-17674838 None:intergenic 40.0%
CTGGATAATCATCAAGTAGC+AGG + chr5.1:17674258-17674277 None:intergenic 40.0%
GAAGAAGAAGAAGATTCCAC+AGG + chr5.1:17672886-17672905 None:intergenic 40.0%
GAAGTTGTAGTTGTCAACCA+TGG + chr5.1:17675117-17675136 None:intergenic 40.0%
GACCAAAAACAAATGTCGGT+TGG + chr5.1:17674148-17674167 None:intergenic 40.0%
GACTACACGTTGGATCATTA+AGG + chr5.1:17674826-17674845 None:intergenic 40.0%
GATCCTTATCATGTGTGTGT+GGG - chr5.1:17673615-17673634 MS.gene72850:intron 40.0%
GCAAAAAATGGAGATGTGCT+AGG - chr5.1:17672794-17672813 MS.gene72850:CDS 40.0%
GCCAAACCCAATTAAGTGAT+TGG + chr5.1:17673773-17673792 None:intergenic 40.0%
GGTTATGACTTCACTTCAGA+TGG - chr5.1:17675026-17675045 MS.gene72850:CDS 40.0%
GTGTTAAGTAAACATCGCGA+TGG + chr5.1:17674924-17674943 None:intergenic 40.0%
GTTATGGGATGACATGTTGA+AGG - chr5.1:17674311-17674330 MS.gene72850:intron 40.0%
GTTGTATAACCCTCTAGTTG+CGG - chr5.1:17673218-17673237 MS.gene72850:intron 40.0%
GTTGTGTATTCGTGTGTGAA+TGG - chr5.1:17673361-17673380 MS.gene72850:intron 40.0%
TAATTGGGTTTGGCATGCTT+TGG - chr5.1:17673779-17673798 MS.gene72850:intron 40.0%
TGATAATCCAGAGTTCGGTT+TGG - chr5.1:17673253-17673272 MS.gene72850:intron 40.0%
TGATGATTATCCAGAAAGGG+CGG - chr5.1:17674264-17674283 MS.gene72850:intron 40.0%
TGTAGGATCTGCGTATGAAA+TGG - chr5.1:17674748-17674767 MS.gene72850:CDS 40.0%
TGTGTATTCGTGTGTGAATG+GGG - chr5.1:17673363-17673382 MS.gene72850:intron 40.0%
TTCCTAGATCCTTCTGTAAG+AGG + chr5.1:17674992-17675011 None:intergenic 40.0%
TTGTATAACCCTCTAGTTGC+GGG - chr5.1:17673219-17673238 MS.gene72850:intron 40.0%
! ATAGATTTGAGCCGGTGTTA+TGG - chr5.1:17674295-17674314 MS.gene72850:intron 40.0%
! ATCAAGGAATGGTTCTACCA+TGG - chr5.1:17675097-17675116 MS.gene72850:intron 40.0%
! CTAGGAACTGTGAAGTATCA+AGG - chr5.1:17675005-17675024 MS.gene72850:CDS 40.0%
! CTGACCTCCTTTTACCTATT+CGG - chr5.1:17672594-17672613 MS.gene72850:CDS 40.0%
!! ATGTTCTAGCTCTTGCCATT+GGG + chr5.1:17672528-17672547 None:intergenic 40.0%
!! TAGATTTGAGCCGGTGTTAT+GGG - chr5.1:17674296-17674315 MS.gene72850:intron 40.0%
!! TTTGAGGTGTTTTGCCTTGT+GGG - chr5.1:17673450-17673469 MS.gene72850:intron 40.0%
!!! TCTAGCCATAGTTTTGGAGT+TGG - chr5.1:17673666-17673685 MS.gene72850:intron 40.0%
!!! TTTTGAGGTGTTTTGCCTTG+TGG - chr5.1:17673449-17673468 MS.gene72850:intron 40.0%
AAATTGACCCAGAACCAGGT+AGG - chr5.1:17672755-17672774 MS.gene72850:CDS 45.0%
ACTTCACTTCAGATGGCATG+TGG - chr5.1:17675033-17675052 MS.gene72850:CDS 45.0%
AGATTTAGCCTCCTCTGCAA+TGG - chr5.1:17675277-17675296 MS.gene72850:CDS 45.0%
AGCGGTTCATAGATTTGAGC+CGG - chr5.1:17674287-17674306 MS.gene72850:intron 45.0%
AGTCACTGAAGAGAGACTGA+GGG + chr5.1:17675244-17675263 None:intergenic 45.0%
ATACCGTTCAAGAAAGCCTG+TGG - chr5.1:17672867-17672886 MS.gene72850:intron 45.0%
ATAGGTAAAAGGAGGTCAGG+AGG + chr5.1:17672593-17672612 None:intergenic 45.0%
ATCGTCTTTAACGGCCAGTT+AGG - chr5.1:17675194-17675213 MS.gene72850:CDS 45.0%
CATTTCCTCAAAACCCCACA+AGG + chr5.1:17673467-17673486 None:intergenic 45.0%
CCAGTTAGGTAGCTTGAAAC+AGG - chr5.1:17675208-17675227 MS.gene72850:CDS 45.0%
CCTATGCTTCTTCCTCTTCA+GGG - chr5.1:17673111-17673130 MS.gene72850:intron 45.0%
CCTGTTTCAAGCTACCTAAC+TGG + chr5.1:17675211-17675230 None:intergenic 45.0%
CGAATAGGTAAAAGGAGGTC+AGG + chr5.1:17672596-17672615 None:intergenic 45.0%
CGGTCACAGTACTTAGAATC+AGG + chr5.1:17672829-17672848 None:intergenic 45.0%
CTTATCTCCAAACCGAACTC+TGG + chr5.1:17673263-17673282 None:intergenic 45.0%
GACCCTGATAATCCAGAGTT+CGG - chr5.1:17673248-17673267 MS.gene72850:intron 45.0%
GACGGAACACATTGTAGTCT+TGG - chr5.1:17674492-17674511 MS.gene72850:intron 45.0%
GCCGTTAAAGACGATTTCCT+TGG + chr5.1:17675189-17675208 None:intergenic 45.0%
GGAGAACAACCTCTTACAGA+AGG - chr5.1:17674980-17674999 MS.gene72850:CDS 45.0%
GGATCCTTATCATGTGTGTG+TGG - chr5.1:17673614-17673633 MS.gene72850:intron 45.0%
GGTAGGTGTAGAAGAACAGA+TGG - chr5.1:17672772-17672791 MS.gene72850:CDS 45.0%
GGTTTCTTAGAACCCTGAAG+AGG + chr5.1:17673126-17673145 None:intergenic 45.0%
GTGAAACGAACATGCTGTAG+TGG - chr5.1:17674608-17674627 MS.gene72850:intron 45.0%
GTTGTTGGGTACTTACGAGA+TGG + chr5.1:17672644-17672663 None:intergenic 45.0%
TCGTTTCACAGCCATCTACA+TGG + chr5.1:17674597-17674616 None:intergenic 45.0%
TCTGTTCTTCTACACCTACC+TGG + chr5.1:17672772-17672791 None:intergenic 45.0%
TGGGGTGAGATTTGAGAGTA+AGG - chr5.1:17673634-17673653 MS.gene72850:intron 45.0%
TGTATAACCCTCTAGTTGCG+GGG - chr5.1:17673220-17673239 MS.gene72850:intron 45.0%
! AAGAAGCATAGGGTCCATGT+GGG + chr5.1:17673103-17673122 None:intergenic 45.0%
! CCTGAAGAGGAAGAAGCATA+GGG + chr5.1:17673113-17673132 None:intergenic 45.0%
! TTCTACACCTACCTGGTTCT+GGG + chr5.1:17672765-17672784 None:intergenic 45.0%
! TTTTGCCTTGTGGGGTTTTG+AGG - chr5.1:17673459-17673478 MS.gene72850:intron 45.0%
!! GATGTTCTAGCTCTTGCCAT+TGG + chr5.1:17672529-17672548 None:intergenic 45.0%
!! TGAGTTGGTGACCACATCAA+CGG + chr5.1:17672488-17672507 None:intergenic 45.0%
!! TTGAGGTGTTTTGCCTTGTG+GGG - chr5.1:17673451-17673470 MS.gene72850:intron 45.0%
AATGGAGTACAGGCCAAGCA+AGG - chr5.1:17675295-17675314 MS.gene72850:CDS 50.0%
ACTACCAGAGCCTCTTCTCT+TGG + chr5.1:17674186-17674205 None:intergenic 50.0%
ACTGTGAACGGTGGATGAGT+TGG + chr5.1:17672503-17672522 None:intergenic 50.0%
ATTATCCAGAAAGGGCGGAG+CGG - chr5.1:17674269-17674288 MS.gene72850:intron 50.0%
CACCCCACACACACATGATA+AGG + chr5.1:17673621-17673640 None:intergenic 50.0%
CAGGTTCTCTTGTTCGCTGT+TGG + chr5.1:17675139-17675158 None:intergenic 50.0%
CAGTCACTGAAGAGAGACTG+AGG + chr5.1:17675245-17675264 None:intergenic 50.0%
CCCTATGCTTCTTCCTCTTC+AGG - chr5.1:17673110-17673129 MS.gene72850:intron 50.0%
CTACCAGAGCCTCTTCTCTT+GGG + chr5.1:17674185-17674204 None:intergenic 50.0%
CTCAGTCTCTCTTCAGTGAC+TGG - chr5.1:17675243-17675262 MS.gene72850:CDS 50.0%
CTTCTACACCTACCTGGTTC+TGG + chr5.1:17672766-17672785 None:intergenic 50.0%
GTATAACCCTCTAGTTGCGG+GGG - chr5.1:17673221-17673240 MS.gene72850:intron 50.0%
GTCACTGAAGAGAGACTGAG+GGG + chr5.1:17675243-17675262 None:intergenic 50.0%
TATAACCCTCTAGTTGCGGG+GGG - chr5.1:17673222-17673241 MS.gene72850:intron 50.0%
TGCCATTGGGAGACTGTGAA+CGG + chr5.1:17672515-17672534 None:intergenic 50.0%
TGTTTAACCTCGACGCTGCA+AGG - chr5.1:17675162-17675181 MS.gene72850:intron 50.0%
TTCAACAACCGCCGTTGATG+TGG - chr5.1:17672474-17672493 MS.gene72850:CDS 50.0%
! AGAAGCATAGGGTCCATGTG+GGG + chr5.1:17673102-17673121 None:intergenic 50.0%
! CCCTGAAGAGGAAGAAGCAT+AGG + chr5.1:17673114-17673133 None:intergenic 50.0%
! GAAGAAGCATAGGGTCCATG+TGG + chr5.1:17673104-17673123 None:intergenic 50.0%
! GGTTAGTTATGGCCGAACTC+CGG + chr5.1:17674209-17674228 None:intergenic 50.0%
!! ACTTCACAGCAACTTGGTGC+TGG - chr5.1:17672955-17672974 MS.gene72850:intron 50.0%
CACCGTTCACAGTCTCCCAA+TGG - chr5.1:17672510-17672529 MS.gene72850:CDS 55.0%
CATAGGGTCCATGTGGGGTA+TGG + chr5.1:17673097-17673116 None:intergenic 55.0%
CATTGGGAGACTGTGAACGG+TGG + chr5.1:17672512-17672531 None:intergenic 55.0%
CCTCCTCTGCAATGGAGTAC+AGG - chr5.1:17675285-17675304 MS.gene72850:CDS 55.0%
CCTCTTCTCTTGGGAACCAG+AGG + chr5.1:17674176-17674195 None:intergenic 55.0%
CCTGTACTCCATTGCAGAGG+AGG + chr5.1:17675288-17675307 None:intergenic 55.0%
CTCTCTTCAGTGACTGGCAG+TGG - chr5.1:17675249-17675268 MS.gene72850:CDS 55.0%
GCGTCGAGGTTAAACAGCTC+AGG + chr5.1:17675158-17675177 None:intergenic 55.0%
GTTCCCAAGAGAAGAGGCTC+TGG - chr5.1:17674179-17674198 MS.gene72850:intron 55.0%
TGGACTGAACTCGGAAGCAG+AGG - chr5.1:17674959-17674978 MS.gene72850:CDS 55.0%
TGGCCTGTACTCCATTGCAG+AGG + chr5.1:17675291-17675310 None:intergenic 55.0%
TGGGAACACCATACCCCACA+TGG - chr5.1:17673086-17673105 MS.gene72850:intron 55.0%
! CCTCTGGTTCCCAAGAGAAG+AGG - chr5.1:17674173-17674192 MS.gene72850:intron 55.0%
! GAGAAGAGGCTCTGGTAGTC+CGG - chr5.1:17674187-17674206 MS.gene72850:intron 55.0%
!! CTTGGTGCTGGTGGTTCTAG+TGG - chr5.1:17672967-17672986 MS.gene72850:intron 55.0%
!! GTTGGTGACCACATCAACGG+CGG + chr5.1:17672485-17672504 None:intergenic 55.0%
!! TCACAGCAACTTGGTGCTGG+TGG - chr5.1:17672958-17672977 MS.gene72850:intron 55.0%
ATGAACCGCTCCGCCCTTTC+TGG + chr5.1:17674277-17674296 None:intergenic 60.0%
! GGCTCTGGTAGTCCGGAGTT+CGG - chr5.1:17674194-17674213 MS.gene72850:intron 60.0%
ACCCTCTAGTTGCGGGGGGA+CGG - chr5.1:17673226-17673245 MS.gene72850:intron 65.0%
CCTCGACGCTGCAAGGTCCA+AGG - chr5.1:17675169-17675188 MS.gene72850:intron 65.0%
CCTTGGACCTTGCAGCGTCG+AGG + chr5.1:17675172-17675191 None:intergenic 65.0%
TCCGTCCCCCCGCAACTAGA+GGG + chr5.1:17673230-17673249 None:intergenic 65.0%
GTCCGTCCCCCCGCAACTAG+AGG + chr5.1:17673231-17673250 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 17672467 17675352 17672467 ID=MS.gene72850
chr5.1 mRNA 17672467 17675352 17672467 ID=MS.gene72850.t1;Parent=MS.gene72850
chr5.1 exon 17675172 17675352 17675172 ID=MS.gene72850.t1.exon1;Parent=MS.gene72850.t1
chr5.1 CDS 17675172 17675352 17675172 ID=cds.MS.gene72850.t1;Parent=MS.gene72850.t1
chr5.1 exon 17674668 17675082 17674668 ID=MS.gene72850.t1.exon2;Parent=MS.gene72850.t1
chr5.1 CDS 17674668 17675082 17674668 ID=cds.MS.gene72850.t1;Parent=MS.gene72850.t1
chr5.1 exon 17672467 17672863 17672467 ID=MS.gene72850.t1.exon3;Parent=MS.gene72850.t1
chr5.1 CDS 17672467 17672863 17672467 ID=cds.MS.gene72850.t1;Parent=MS.gene72850.t1
Gene Sequence

>MS.gene72850

ATGAGCATTCAACAACCGCCGTTGATGTGGTCACCAACTCATCCACCGTTCACAGTCTCCCAATGGCAAGAGCTAGAACATCAAGCTCTCATCTTCAAGTATCTGAAAGCTGGTTTATCTGTTCCTCCTGACCTCCTTTTACCTATTCGGAAAAGTCTTCAATTCATGTCTCATCCATCTCGTAAGTACCCAACAACGTTAATCTTCCTTATTTTGTCTCTATTTTGATTTACAACAATAAAGTTTTGATTTTTGTGATTTTTGTTTCAGTGGGTTATTATGGAAAAAAAATTGACCCAGAACCAGGTAGGTGTAGAAGAACAGATGGCAAAAAATGGAGATGTGCTAGGGATGCACATCCTGATTCTAAGTACTGTGACCGTCATATGATTCGACGTCGATACCGTTCAAGAAAGCCTGTGGAATCTTCTTCTTCTTCTTCTTGTTCACAATCTTCATCTTCAGTTTCAGTTTCTACTACTAGTACTACTTCACAGCAACTTGGTGCTGGTGGTTCTAGTGGAAGTACTAGTACTGTTTCTGCTTCTGTTACTGCTACAACTTTTCAAACACTTCCTTTGCATACAAATGGTACTCGTGAAGGTTTAAGTTTCAATTTTGGGAACACCATACCCCACATGGACCCTATGCTTCTTCCTCTTCAGGGTTCTAAGAAACCTTATAGGTATGTTTCTTTTTTTGATTCATTTTGTTTTTTTTTTTTGTTTTCAATATGGCAACACTTTCTTATGTTGTATAACCCTCTAGTTGCGGGGGGACGGACCCTGATAATCCAGAGTTCGGTTTGGAGATAAGTAAAGTCTGGCCAAGAATTATTCAATTCAGGATTTGCTATTTTTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTGTATTCGTGTGTGAATGGGGTATTTTCACTTTTTGATGTTTGATTTTTTTATTTGTTGTGTGTTCAATGTTTGTGTATTTGTTTTTTGAGGTGTTTTGCCTTGTGGGGTTTTGAGGAAATGATTGCCATGATTTCTTGCGAAGTGTTATATTGTTGTTCTTCCTTTTTGTTTACACAATTGATTTTATATCTTTATGTTAAATGGAGAATATGGTAGTTGTGATGTTAAGTTACATAACATGTGATGTGGATCCTTATCATGTGTGTGTGGGGTGAGATTTGAGAGTAAGGTGCAAGTCATCTAGCCATAGTTTTGGAGTTGGCTTGTACTATATTTTGTTTTGACAAAGGCTTATACTTTATTTTATTCAACTTGGTTTGTGTGAATATGAAAGAATGCAGTACCAATCACTTAATTGGGTTTGGCATGCTTTGGTAAAAGATTTTGTGCATTGGATTTAATTAGGTAATGCAGTGTATCATCTAAAATTTGCTTAACTCTTGACTTCATTTTAAACTGAATTGCCTTTTTGATGGTCATTTGAAATCATGATTCGGTATTCCAGGTTATTACTTGTTCAAATATTTTGTTGAAATTCTTCACACATTTTATTTGATATCTTGTTCAAATATTTTGAGTGAACTAAGAGGCATGATGTGATAATATTGGAAATCAAGGGAATTGTGGATGATTGTATAACTGAAACAATAACAAGAGAACTGATTTTTTGTGTGTGAGAGTTAAAGCAATTAAGTAGAGGTAATTTCACATTAGTCAACCAACCGACATTTGTTTTTGGTCTTAATCCTCTGGTTCCCAAGAGAAGAGGCTCTGGTAGTCCGGAGTTCGGCCATAACTAACCAACATCTAACTAGCATCTAACTGCTGCCTGCTACTTGATGATTATCCAGAAAGGGCGGAGCGGTTCATAGATTTGAGCCGGTGTTATGGGATGACATGTTGAAGGAATTTGGAAATGTGCTATTTCTTTTTATATCTATTTGGCGATATGATTTTTTAATTCTAGGCCATCAATTAGATGTCGTGATATCTTTCATTATTATTTGATGTCTTTCTTCTTCCTCCTACTTGCATTCTTGTTTGACTGTTTATTCTCTACTTTTAGACGGAACACATTGTAGTCTTGGCTACAGCTACTTAAGTGTTCAATGCGATCTGCTGTTTTTTTTCCTTTTCCTTGTTCAGCAATGTACATTGTCATTGATACCATGTAGATGGCTGTGAAACGAACATGCTGTAGTGGAATTTTTGTTTAGTTTTTTAGATTTAAAAAAGAAAAGAAGAGAAATGGAGCGAACATTATAGCAAGAGTTGTATTTTGGTAATTTGAAGCAAGTGATTTTACTTTTTATAAGGAACTTGTAGGATCTGCGTATGAAATGGATATTAATATTTTTATGAAGGTGGATTAAGGAAGCATCTAAACTGCCTTAAGCCTTAATGATCCAACGTGTAGTCTTTTGCAATTTTTTTCTTAAGTATTATCAGAAGTTGTTGGTAATTTTCTATCATGCACACAAGTGAAAAAACGTACCATCGCGATGTTTACTTAACACACTCAATAATAGGTTTGGACTGAACTCGGAAGCAGAGGAGAACAACCTCTTACAGAAGGATCTAGGAACTGTGAAGTATCAAGGTTATGACTTCACTTCAGATGGCATGTGGTATAATAACATGTCTCAGATTTCATCAAACACTGTTTCAGAATCAAGGAATGGTTCTACCATGGTTGACAACTACAACTTCCAACAGCGAACAAGAGAACCTGAGCTGTTTAACCTCGACGCTGCAAGGTCCAAGGAAATCGTCTTTAACGGCCAGTTAGGTAGCTTGAAACAGGAATATCAATCCCCTCAGTCTCTCTTCAGTGACTGGCAGTGGAAGAAAGATTTAGCCTCCTCTGCAATGGAGTACAGGCCAAGCAAGGATTTTAACACCAATCCAGATGTCAATGTTGATTGA
Protein sequence

>MS.gene72850.t1

MSIQQPPLMWSPTHPPFTVSQWQELEHQALIFKYLKAGLSVPPDLLLPIRKSLQFMSHPSLGYYGKKIDPEPGRCRRTDGKKWRCARDAHPDSKYCDRHMIRRRYRSRKPVESSSSSSCSQSSSSVSVSTTSTTSQQLGAGGSSGSTSTVSASVTATTFQTLPLHTNGTREGLSFNFGNTIPHMDPMLLPLQGSKKPYRFGLNSEAEENNLLQKDLGTVKYQGYDFTSDGMWYNNMSQISSNTVSESRNGSTMVDNYNFQQRTREPELFNLDAARSKEIVFNGQLGSLKQEYQSPQSLFSDWQWKKDLASSAMEYRPSKDFNTNPDVNVD