Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73395.t1 | RHN47337.1 | 99 | 198 | 2 | 0 | 1 | 198 | 7 | 204 | 2.50E-108 | 401.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73395.t1 | Q9LW31 | 52.1 | 240 | 65 | 5 | 1 | 198 | 82 | 313 | 8.5e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73395.t1 | Q2HUJ5 | 99.0 | 198 | 2 | 0 | 1 | 198 | 117 | 314 | 1.8e-108 | 401.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene73395.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73395.t1 | MTR_7g083700 | 98.990 | 198 | 2 | 0 | 1 | 198 | 117 | 314 | 5.40e-148 | 413 |
| MS.gene73395.t1 | MTR_7g059330 | 52.586 | 116 | 55 | 0 | 5 | 120 | 618 | 733 | 2.56e-34 | 128 |
| MS.gene73395.t1 | MTR_2g011940 | 41.463 | 123 | 63 | 3 | 10 | 127 | 352 | 470 | 7.42e-21 | 90.1 |
| MS.gene73395.t1 | MTR_2g011940 | 41.463 | 123 | 63 | 3 | 10 | 127 | 352 | 470 | 7.48e-21 | 90.1 |
| MS.gene73395.t1 | MTR_2g011940 | 41.463 | 123 | 63 | 3 | 10 | 127 | 343 | 461 | 9.26e-21 | 89.7 |
| MS.gene73395.t1 | MTR_5g075410 | 49.412 | 85 | 42 | 1 | 10 | 94 | 321 | 404 | 3.74e-20 | 87.8 |
| MS.gene73395.t1 | MTR_3g467380 | 47.059 | 85 | 44 | 1 | 10 | 94 | 338 | 421 | 3.88e-20 | 87.8 |
| MS.gene73395.t1 | MTR_4g088605 | 40.179 | 112 | 65 | 2 | 3 | 114 | 98 | 207 | 3.90e-20 | 85.9 |
| MS.gene73395.t1 | MTR_4g028130 | 39.048 | 105 | 63 | 1 | 10 | 114 | 314 | 417 | 4.38e-20 | 87.8 |
| MS.gene73395.t1 | MTR_4g028130 | 39.048 | 105 | 63 | 1 | 10 | 114 | 314 | 417 | 5.76e-20 | 87.4 |
| MS.gene73395.t1 | MTR_4g028130 | 39.048 | 105 | 63 | 1 | 10 | 114 | 314 | 417 | 6.76e-20 | 87.4 |
| MS.gene73395.t1 | MTR_7g073240 | 37.864 | 103 | 63 | 1 | 10 | 112 | 121 | 222 | 9.71e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73395.t1 | AT3G26790 | 54.741 | 232 | 71 | 6 | 1 | 198 | 82 | 313 | 9.52e-79 | 238 |
| MS.gene73395.t1 | AT1G28300 | 50.000 | 126 | 63 | 0 | 3 | 128 | 163 | 288 | 9.06e-36 | 129 |
| MS.gene73395.t1 | AT3G24650 | 57.944 | 107 | 45 | 0 | 5 | 111 | 566 | 672 | 5.14e-35 | 130 |
| MS.gene73395.t1 | AT2G30470 | 39.048 | 105 | 63 | 1 | 10 | 114 | 294 | 397 | 4.90e-21 | 90.5 |
| MS.gene73395.t1 | AT4G32010 | 34.706 | 170 | 95 | 5 | 10 | 176 | 281 | 437 | 1.19e-20 | 89.4 |
| MS.gene73395.t1 | AT4G32010 | 34.706 | 170 | 95 | 5 | 10 | 176 | 285 | 441 | 1.19e-20 | 89.4 |
| MS.gene73395.t1 | AT4G21550 | 34.848 | 132 | 79 | 4 | 10 | 141 | 327 | 451 | 3.83e-18 | 82.0 |
| MS.gene73395.t1 | AT4G21550 | 34.848 | 132 | 79 | 4 | 10 | 141 | 327 | 451 | 4.59e-18 | 82.0 |
| MS.gene73395.t1 | AT4G21550 | 34.848 | 132 | 79 | 4 | 10 | 141 | 327 | 451 | 2.57e-17 | 79.7 |
| MS.gene73395.t1 | AT1G13260 | 35.897 | 117 | 67 | 5 | 5 | 116 | 182 | 295 | 1.28e-11 | 62.8 |
| MS.gene73395.t1 | AT4G01500 | 36.170 | 94 | 56 | 3 | 6 | 98 | 31 | 121 | 5.27e-11 | 60.8 |
Find 32 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTGTTCTTCAACTCCTTT+TGG | 0.172423 | 7.1:-21410678 | None:intergenic |
| TGATCCAATTCTTGAGTAAT+TGG | 0.227933 | 7.1:-21411744 | None:intergenic |
| ATTATCTTGGTAAACCATAA+TGG | 0.319330 | 7.1:-21411386 | None:intergenic |
| ATTGGATCAGTTGAAACATT+TGG | 0.328924 | 7.1:+21411758 | MS.gene73395:CDS |
| TACATTCGACTGTTATTGTT+AGG | 0.354796 | 7.1:-21411218 | None:intergenic |
| TGCATTACTACAGGTAATTC+AGG | 0.355023 | 7.1:+21411523 | MS.gene73395:intron |
| ATGACCAATTACTCAAGAAT+TGG | 0.363460 | 7.1:+21411740 | MS.gene73395:CDS |
| ACGCTTTGGAGACTCCATTA+TGG | 0.373590 | 7.1:+21411372 | MS.gene73395:CDS |
| GAATTCCTTCCTTCGATTCA+AGG | 0.374741 | 7.1:-21410881 | None:intergenic |
| AGCACACATGGTCTACGCTT+TGG | 0.436188 | 7.1:+21411358 | MS.gene73395:CDS |
| TTGTGATCAAGATGAGTATA+TGG | 0.437591 | 7.1:+21411556 | MS.gene73395:CDS |
| CCTTCCTTCGATTCAAGGAC+AGG | 0.443951 | 7.1:-21410876 | None:intergenic |
| GTTTATAGGAAATCGATCAT+AGG | 0.469630 | 7.1:+21410638 | None:intergenic |
| ATATAGATGGTCTTCATGTA+TGG | 0.471743 | 7.1:+21410919 | MS.gene73395:CDS |
| TATCTTCGTCGATGATTATG+AGG | 0.472524 | 7.1:+21411604 | MS.gene73395:CDS |
| TCTCTTGATGACTTCTATTG+AGG | 0.486619 | 7.1:+21411797 | MS.gene73395:CDS |
| ATGTATGGAGTTTCAAGTAC+AGG | 0.489515 | 7.1:+21410934 | MS.gene73395:CDS |
| CTCAGCATGAATGATATAGA+TGG | 0.492894 | 7.1:+21410906 | MS.gene73395:CDS |
| ATACTTGTATCATTCATTGC+AGG | 0.501561 | 7.1:-21411656 | None:intergenic |
| CCTTCCTGTCCTTGAATCGA+AGG | 0.505006 | 7.1:+21410872 | MS.gene73395:CDS |
| TAGGAGAATGGTGTTGCCTA+AGG | 0.511895 | 7.1:+21410714 | MS.gene73395:CDS |
| TAGGCAACACCATTCTCCTA+AGG | 0.515359 | 7.1:-21410711 | None:intergenic |
| TGTTAGCTCCCTTAGGAGAA+TGG | 0.536299 | 7.1:+21410702 | MS.gene73395:CDS |
| GGAGACTTTGTTAGCACACA+TGG | 0.544519 | 7.1:+21411346 | MS.gene73395:intron |
| ACAGTGATGTTAGCTCCCTT+AGG | 0.583324 | 7.1:+21410695 | MS.gene73395:CDS |
| CCTGTCCTTGAATCGAAGGA+AGG | 0.585425 | 7.1:+21410876 | MS.gene73395:CDS |
| TATAGGAAATCGATCATAGG+AGG | 0.597683 | 7.1:+21410641 | None:intergenic |
| AGGCAACACCATTCTCCTAA+GGG | 0.607377 | 7.1:-21410710 | None:intergenic |
| CCTTCGATTCAAGGACAGGA+AGG | 0.620428 | 7.1:-21410872 | None:intergenic |
| ATGTATGTACTTGAAAACAC+TGG | 0.631186 | 7.1:+21411236 | MS.gene73395:CDS |
| AAGTGGTGAGTCATTTGAGA+TGG | 0.633283 | 7.1:-21411699 | None:intergenic |
| TAACTTGCAGAAAGCAGCAG+AGG | 0.635910 | 7.1:+21410845 | MS.gene73395:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAATTTATTATATTATA+AGG | - | chr7.1:21410824-21410843 | None:intergenic | 0.0% |
| !! | AAATTTAATGCAATTACATT+CGG | + | chr7.1:21411015-21411034 | MS.gene73395:intron | 15.0% |
| !! | ATTATATTATAAGGTTATGA+TGG | - | chr7.1:21410815-21410834 | None:intergenic | 15.0% |
| !!! | TTAGTTTGTTAAATATTTTC+AGG | + | chr7.1:21411325-21411344 | MS.gene73395:intron | 15.0% |
| !! | AATTGCATTAAATTTCATTC+TGG | - | chr7.1:21411010-21411029 | None:intergenic | 20.0% |
| !!! | AATTGTACTTTAAAGTCATT+TGG | + | chr7.1:21411119-21411138 | MS.gene73395:intron | 20.0% |
| !!! | AATTGTGATTTTGATTATCT+TGG | - | chr7.1:21411402-21411421 | None:intergenic | 20.0% |
| !!! | ATTGTACTTTAAAGTCATTT+GGG | + | chr7.1:21411120-21411139 | MS.gene73395:intron | 20.0% |
| ! | ATTATCTTGGTAAACCATAA+TGG | - | chr7.1:21411389-21411408 | None:intergenic | 25.0% |
| ! | TGACAGGAAAAAAAAATACA+TGG | - | chr7.1:21411501-21411520 | None:intergenic | 25.0% |
| !! | TATTTCTATTTCAATACTGC+AGG | + | chr7.1:21411189-21411208 | MS.gene73395:intron | 25.0% |
| AGCAAAGTTTAACTCTCAAA+TGG | - | chr7.1:21411295-21411314 | None:intergenic | 30.0% | |
| ATACTTGTATCATTCATTGC+AGG | - | chr7.1:21411659-21411678 | None:intergenic | 30.0% | |
| ATATAGATGGTCTTCATGTA+TGG | + | chr7.1:21410919-21410938 | MS.gene73395:CDS | 30.0% | |
| ATGACCAATTACTCAAGAAT+TGG | + | chr7.1:21411740-21411759 | MS.gene73395:CDS | 30.0% | |
| ATGTATGTACTTGAAAACAC+TGG | + | chr7.1:21411236-21411255 | MS.gene73395:CDS | 30.0% | |
| ATTGGATCAGTTGAAACATT+TGG | + | chr7.1:21411758-21411777 | MS.gene73395:CDS | 30.0% | |
| GAAATGAAGTCTATAAGCAA+AGG | - | chr7.1:21410745-21410764 | None:intergenic | 30.0% | |
| GTCAATCATTCTTTATCTGA+CGG | - | chr7.1:21411089-21411108 | None:intergenic | 30.0% | |
| TACATTCGACTGTTATTGTT+AGG | - | chr7.1:21411221-21411240 | None:intergenic | 30.0% | |
| TGATCCAATTCTTGAGTAAT+TGG | - | chr7.1:21411747-21411766 | None:intergenic | 30.0% | |
| TTGTGATCAAGATGAGTATA+TGG | + | chr7.1:21411556-21411575 | MS.gene73395:CDS | 30.0% | |
| ! | TATTTCAATACTGCAGGTTT+TGG | + | chr7.1:21411195-21411214 | MS.gene73395:intron | 30.0% |
| CTCAGCATGAATGATATAGA+TGG | + | chr7.1:21410906-21410925 | MS.gene73395:CDS | 35.0% | |
| TATCTTCGTCGATGATTATG+AGG | + | chr7.1:21411604-21411623 | MS.gene73395:CDS | 35.0% | |
| TGCATTACTACAGGTAATTC+AGG | + | chr7.1:21411523-21411542 | MS.gene73395:intron | 35.0% | |
| ! | ATGTATGGAGTTTCAAGTAC+AGG | + | chr7.1:21410934-21410953 | MS.gene73395:CDS | 35.0% |
| ! | GTTGAGATTCCTTTTCCAAA+AGG | + | chr7.1:21410663-21410682 | MS.gene73395:CDS | 35.0% |
| !! | TCTTCAACTCCTTTTGGAAA+AGG | - | chr7.1:21410675-21410694 | None:intergenic | 35.0% |
| !!! | GACTCACCACTTGATTTTTT+GGG | + | chr7.1:21411710-21411729 | MS.gene73395:CDS | 35.0% |
| !!! | TGACTCACCACTTGATTTTT+TGG | + | chr7.1:21411709-21411728 | MS.gene73395:CDS | 35.0% |
| AAGTGGTGAGTCATTTGAGA+TGG | - | chr7.1:21411702-21411721 | None:intergenic | 40.0% | |
| ATAAGCAAAGGTCAGACCTT+AGG | - | chr7.1:21410733-21410752 | None:intergenic | 40.0% | |
| CCTGTAGTAATGCATATGAC+AGG | - | chr7.1:21411517-21411536 | None:intergenic | 40.0% | |
| CCTGTCATATGCATTACTAC+AGG | + | chr7.1:21411514-21411533 | MS.gene73395:intron | 40.0% | |
| GAATTCCTTCCTTCGATTCA+AGG | - | chr7.1:21410884-21410903 | None:intergenic | 40.0% | |
| GATCCACCCAAAAAATCAAG+TGG | - | chr7.1:21411719-21411738 | None:intergenic | 40.0% | |
| TGTACAGTCACACACTTACA+CGG | + | chr7.1:21411050-21411069 | MS.gene73395:intron | 40.0% | |
| ! | CACTGTTCTTCAACTCCTTT+TGG | - | chr7.1:21410681-21410700 | None:intergenic | 40.0% |
| !!! | TCACCACTTGATTTTTTGGG+TGG | + | chr7.1:21411713-21411732 | MS.gene73395:CDS | 40.0% |
| ACAGTGATGTTAGCTCCCTT+AGG | + | chr7.1:21410695-21410714 | MS.gene73395:CDS | 45.0% | |
| AGGCAACACCATTCTCCTAA+GGG | - | chr7.1:21410713-21410732 | None:intergenic | 45.0% | |
| CTGCTGTGTGCATACATTGT+TGG | + | chr7.1:21410958-21410977 | MS.gene73395:intron | 45.0% | |
| GGAGACTTTGTTAGCACACA+TGG | + | chr7.1:21411346-21411365 | MS.gene73395:intron | 45.0% | |
| TAGGCAACACCATTCTCCTA+AGG | - | chr7.1:21410714-21410733 | None:intergenic | 45.0% | |
| TGTTAGCTCCCTTAGGAGAA+TGG | + | chr7.1:21410702-21410721 | MS.gene73395:CDS | 45.0% | |
| ! | TAACTTGCAGAAAGCAGCAG+AGG | + | chr7.1:21410845-21410864 | MS.gene73395:intron | 45.0% |
| !! | ACGCTTTGGAGACTCCATTA+TGG | + | chr7.1:21411372-21411391 | MS.gene73395:CDS | 45.0% |
| !! | TAGGAGAATGGTGTTGCCTA+AGG | + | chr7.1:21410714-21410733 | MS.gene73395:CDS | 45.0% |
| AGCACACATGGTCTACGCTT+TGG | + | chr7.1:21411358-21411377 | MS.gene73395:CDS | 50.0% | |
| CCTGTCCTTGAATCGAAGGA+AGG | + | chr7.1:21410876-21410895 | MS.gene73395:CDS | 50.0% | |
| CCTTCCTGTCCTTGAATCGA+AGG | + | chr7.1:21410872-21410891 | MS.gene73395:CDS | 50.0% | |
| CCTTCCTTCGATTCAAGGAC+AGG | - | chr7.1:21410879-21410898 | None:intergenic | 50.0% | |
| CCTTCGATTCAAGGACAGGA+AGG | - | chr7.1:21410875-21410894 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 21410646 | 21411817 | 21410646 | ID=MS.gene73395 |
| chr7.1 | mRNA | 21410646 | 21411817 | 21410646 | ID=MS.gene73395.t1;Parent=MS.gene73395 |
| chr7.1 | exon | 21410646 | 21410735 | 21410646 | ID=MS.gene73395.t1.exon1;Parent=MS.gene73395.t1 |
| chr7.1 | CDS | 21410646 | 21410735 | 21410646 | ID=cds.MS.gene73395.t1;Parent=MS.gene73395.t1 |
| chr7.1 | exon | 21410855 | 21410955 | 21410855 | ID=MS.gene73395.t1.exon2;Parent=MS.gene73395.t1 |
| chr7.1 | CDS | 21410855 | 21410955 | 21410855 | ID=cds.MS.gene73395.t1;Parent=MS.gene73395.t1 |
| chr7.1 | exon | 21411211 | 21411257 | 21411211 | ID=MS.gene73395.t1.exon3;Parent=MS.gene73395.t1 |
| chr7.1 | CDS | 21411211 | 21411257 | 21411211 | ID=cds.MS.gene73395.t1;Parent=MS.gene73395.t1 |
| chr7.1 | exon | 21411347 | 21411423 | 21411347 | ID=MS.gene73395.t1.exon4;Parent=MS.gene73395.t1 |
| chr7.1 | CDS | 21411347 | 21411423 | 21411347 | ID=cds.MS.gene73395.t1;Parent=MS.gene73395.t1 |
| chr7.1 | exon | 21411536 | 21411817 | 21411536 | ID=MS.gene73395.t1.exon5;Parent=MS.gene73395.t1 |
| chr7.1 | CDS | 21411536 | 21411817 | 21411536 | ID=cds.MS.gene73395.t1;Parent=MS.gene73395.t1 |
| Gene Sequence |
| Protein sequence |
