AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73436

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73436.t1 MTR_1g109320 99.720 357 1 0 1 357 1 357 0.0 742
MS.gene73436.t1 MTR_5g090040 67.619 105 17 2 241 345 63 150 1.91e-43 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73436.t1 AT1G68200 49.492 295 124 7 62 352 29 302 2.49e-85 261
MS.gene73436.t1 AT1G68200 49.492 295 124 7 62 352 29 302 2.49e-85 261
MS.gene73436.t1 AT1G68200 49.153 295 124 8 62 352 29 301 1.04e-82 254
MS.gene73436.t1 AT1G68200 49.153 295 124 8 62 352 29 301 1.04e-82 254
MS.gene73436.t1 AT1G66810 62.329 146 46 1 200 345 168 304 5.66e-56 185
MS.gene73436.t1 AT1G66810 62.329 146 46 1 200 345 168 304 5.66e-56 185
MS.gene73436.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.54e-22 92.8
MS.gene73436.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.54e-22 92.8

Find 106 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCAGGATGGTCCTTGC+TGG 0.159266 1.4:-83713158 MS.gene73436:CDS
CTGCAGAGCTTCAACTTCTT+TGG 0.179049 1.4:+83715727 None:intergenic
TCTTTCCAGCTTCATTGATC+TGG 0.216499 1.4:+83713055 None:intergenic
GCCGCCGTTGCTGCTGTTTC+CGG 0.266911 1.4:+83715868 None:intergenic
GCCGATGCCGTAACCGCTTT+TGG 0.267264 1.4:+83715979 None:intergenic
CAACTTCTTTGGAACTTTCC+AGG 0.279329 1.4:+83715738 None:intergenic
TAGCGTGGGTGGCGGATCAC+TGG 0.295264 1.4:+83713191 None:intergenic
GATCTAACAGAAATGCTCTT+TGG 0.302932 1.4:+83715398 None:intergenic
GTCAATTCGCTCATGGCATT+GGG 0.349776 1.4:-83713223 MS.gene73436:CDS
GGTTTCCGTGGCTTGAATCT+TGG 0.355570 1.4:-83715563 MS.gene73436:CDS
CTTTCCAGCTTCATTGATCT+GGG 0.356760 1.4:+83713056 None:intergenic
CATGACGGAAATGGCATCTA+TGG 0.367595 1.4:+83713117 None:intergenic
CTTTATCCTTACTCTGACCT+TGG 0.377357 1.4:-83715836 MS.gene73436:CDS
GCGGGAGCGAGTAGCGCCTT+TGG 0.377546 1.4:+83715313 None:intergenic
GAAACTGGCACATGCCCTTA+TGG 0.383739 1.4:-83713254 MS.gene73436:CDS
TAATAATAACAAGGAGTTGC+AGG 0.387088 1.4:-83715495 MS.gene73436:CDS
TGACATAGCTTTCTCTTGTT+CGG 0.389780 1.4:+83713088 None:intergenic
CCGTCATCCAAAAGCGGTTA+CGG 0.390482 1.4:-83715986 MS.gene73436:CDS
GATACAGAAGCATCAGGATA+TGG 0.396830 1.4:-83715792 MS.gene73436:CDS
CTGTTTCCGGCGAGCGATGA+TGG 0.396990 1.4:+83715881 None:intergenic
TGTAACAAATGGCAGGAAAC+TGG 0.399409 1.4:-83713269 MS.gene73436:CDS
TCTGCAGGATGGTCCTTGCT+GGG 0.402584 1.4:-83713157 MS.gene73436:CDS
CGGTAAGTGCATGACGGAAA+TGG 0.404017 1.4:+83713108 None:intergenic
CTTCTGCAACTCTTTGTTCA+CGG 0.410248 1.4:+83715682 None:intergenic
CCTTCTCGTTCAAGCTTCGC+TGG 0.421155 1.4:-83715651 MS.gene73436:CDS
TATGTTTCTCAGTCGTCTCC+TGG 0.438714 1.4:-83715756 MS.gene73436:CDS
TGTCAATTCGCTCATGGCAT+TGG 0.444853 1.4:-83713224 MS.gene73436:CDS
CAAACGGAGTAGATTGAGTC+TGG 0.467277 1.4:+83715597 None:intergenic
CTTGAAGCTTGCTCCGCCTC+CGG 0.468503 1.4:-83715366 MS.gene73436:CDS
AATTGACAGTGGTCTCCATA+AGG 0.472418 1.4:+83713239 None:intergenic
CGTGGCTTGAATCTTGGAGA+TGG 0.475661 1.4:-83715557 MS.gene73436:CDS
GAAAGGAAAACTGTGCTGAT+TGG 0.476246 1.4:-83715535 MS.gene73436:CDS
GGCGTTCGACTGCATGTGAC+CGG 0.476954 1.4:+83715946 None:intergenic
AGCATTTCTGTTAGATCCAA+TGG 0.486635 1.4:-83715392 MS.gene73436:CDS
TCCAAAAGCGGTTACGGCAT+CGG 0.490846 1.4:-83715980 MS.gene73436:CDS
CGTCGGACTCTCATCAGATT+CGG 0.492348 1.4:+83715466 None:intergenic
GAAGCTCTGCAGCAGGAAAA+CGG 0.500754 1.4:-83715716 MS.gene73436:CDS
AAACCTTGCAACACATCAAA+CGG 0.507246 1.4:+83715581 None:intergenic
TGAGAATAACGGTGTTGAAA+CGG 0.507485 1.4:-83715435 MS.gene73436:CDS
AGCGTGGGTGGCGGATCACT+GGG 0.510898 1.4:+83713192 None:intergenic
AAATGGCATCTATGGCCATA+CGG 0.511220 1.4:+83713125 None:intergenic
ATGCGTGATACAGAAGCATC+AGG 0.518868 1.4:-83715798 MS.gene73436:CDS
TCGCTGGAGAACCGATTCAA+TGG 0.519751 1.4:-83715635 MS.gene73436:CDS
GCTGGGGTTGTATGCCCGTA+TGG 0.521541 1.4:-83713140 MS.gene73436:CDS
CTCGCCGGAAACAGCAGCAA+CGG 0.524709 1.4:-83715872 MS.gene73436:CDS
GGAAACAGCAGCAACGGCGG+CGG 0.524773 1.4:-83715866 MS.gene73436:CDS
GCCGGAAACAGCAGCAACGG+CGG 0.525054 1.4:-83715869 MS.gene73436:CDS
GCTACAAAACTGAGGTCTGC+AGG 0.527724 1.4:-83713172 MS.gene73436:CDS
AATGGCATCTATGGCCATAC+GGG 0.528487 1.4:+83713126 None:intergenic
TTAGTTACGACGGCCGGAGG+CGG 0.529646 1.4:+83715353 None:intergenic
AGCGAGTAGCGCCTTTGGTG+CGG 0.529924 1.4:+83715318 None:intergenic
AGAAGTTGAAGCTCTGCAGC+AGG 0.529958 1.4:-83715723 MS.gene73436:CDS
ATTGACAGTGGTCTCCATAA+GGG 0.530052 1.4:+83713240 None:intergenic
TAAGCCCAGATCAATGAAGC+TGG 0.530814 1.4:-83713060 MS.gene73436:CDS
AGCAAGCTTCAAGTAGCCAT+TGG 0.531440 1.4:+83715376 None:intergenic
AGACTGAGCTCTGTAACAAA+TGG 0.531908 1.4:-83713280 MS.gene73436:CDS
AGGATCCGCCACCATTGAAT+CGG 0.534519 1.4:+83715624 None:intergenic
GCTCAGTCTTGAACATCCCC+TGG 0.541107 1.4:+83713294 None:intergenic
AACAATGCTTGCCGCACCAA+AGG 0.543012 1.4:-83715329 MS.gene73436:CDS
ACTCCGTTTGATGTGTTGCA+AGG 0.545981 1.4:-83715584 MS.gene73436:CDS
TCTGTTCCAAGGTCAGAGTA+AGG 0.546191 1.4:+83715830 None:intergenic
GATCTTCCATCATCGCTCGC+CGG 0.559218 1.4:-83715887 MS.gene73436:CDS
TGAGCTCTGTAACAAATGGC+AGG 0.566245 1.4:-83713276 MS.gene73436:CDS
AGCATTGTTGTTAGTTACGA+CGG 0.567553 1.4:+83715343 None:intergenic
CATCTCCAAGATTCAAGCCA+CGG 0.567612 1.4:+83715558 None:intergenic
TGTGCGAGGAGGGCAGAAAG+AGG 0.570699 1.4:-83713345 MS.gene73436:CDS
GACCACTGTCAATTCGCTCA+TGG 0.571725 1.4:-83713230 MS.gene73436:CDS
CTGCAGGATGGTCCTTGCTG+GGG 0.572424 1.4:-83713156 MS.gene73436:CDS
AGAGGAAGAGCCTCTTGAGA+TGG 0.573431 1.4:-83713327 MS.gene73436:CDS
AAAAGGTATTTGTGCGAGGA+GGG 0.574881 1.4:-83713355 MS.gene73436:CDS
GAGAACCGATTCAATGGTGG+CGG 0.578241 1.4:-83715629 MS.gene73436:CDS
CCAGCGAAGCTTGAACGAGA+AGG 0.578885 1.4:+83715651 None:intergenic
TACAACTGTATCAGATTGAG+TGG 0.578967 1.4:+83715283 None:intergenic
TGAGATGGTTGTGTACAACC+AGG 0.582022 1.4:-83713312 MS.gene73436:CDS
CTTGAATCTTGGAGATGGAA+AGG 0.582921 1.4:-83715552 MS.gene73436:CDS
CAGATTGAGTGGAGGAAGCG+CGG 0.588323 1.4:+83715294 None:intergenic
CGGAGTAGATTGAGTCTGGA+CGG 0.593339 1.4:+83715601 None:intergenic
GCAACTCTTTGTTCACGGCA+CGG 0.595404 1.4:+83715687 None:intergenic
AGATTGAGTGGAGGAAGCGC+GGG 0.600576 1.4:+83715295 None:intergenic
CAAAAGGTATTTGTGCGAGG+AGG 0.603500 1.4:-83713356 MS.gene73436:CDS
TTGTTGTTAGTTACGACGGC+CGG 0.605294 1.4:+83715347 None:intergenic
CTTGTTCGGTAAGTGCATGA+CGG 0.607121 1.4:+83713102 None:intergenic
GATGTGTTGCAAGGTTTCCG+TGG 0.607851 1.4:-83715575 MS.gene73436:CDS
GGTCACATGCAGTCGAACGC+CGG 0.607925 1.4:-83715944 MS.gene73436:CDS
CAAAACTGAGGTCTGCAGGA+TGG 0.616724 1.4:-83713168 MS.gene73436:CDS
ACGAGTGTGATTGAGAATAA+CGG 0.622937 1.4:-83715446 MS.gene73436:CDS
ATGAGTCGTACTCTGTTCCA+AGG 0.623681 1.4:+83715819 None:intergenic
CAGCAAAAGGTATTTGTGCG+AGG 0.628338 1.4:-83713359 MS.gene73436:CDS
CTGGAGAACCGATTCAATGG+TGG 0.634958 1.4:-83715632 MS.gene73436:CDS
TGTTCTCCGTCATCCAAAAG+CGG 0.637659 1.4:-83715992 MS.gene73436:CDS
GAGATGGTTGTGTACAACCA+GGG 0.638365 1.4:-83713311 MS.gene73436:CDS
TTGTTAGTTACGACGGCCGG+AGG 0.641381 1.4:+83715350 None:intergenic
GGCATCGGCGTCAACAACGC+CGG 0.645707 1.4:-83715965 MS.gene73436:CDS
TCAATTCGCTCATGGCATTG+GGG 0.646224 1.4:-83713222 MS.gene73436:CDS
TTGTACACAACCATCTCAAG+AGG 0.647753 1.4:+83713317 None:intergenic
ACCGATCAAAATGCATAACA+AGG 0.654571 1.4:-83716020 None:intergenic
AACTGTATCAGATTGAGTGG+AGG 0.659126 1.4:+83715286 None:intergenic
TAACATCAATAATAATAACA+AGG 0.675567 1.4:-83715504 MS.gene73436:CDS
TGCCATGAGCGAATTGACAG+TGG 0.682669 1.4:+83713228 None:intergenic
TTATTCTCAATCACACTCGT+CGG 0.688787 1.4:+83715449 None:intergenic
ACGGGCATACAACCCCAGCA+AGG 0.691546 1.4:+83713144 None:intergenic
AGTAGATTGAGTCTGGACGG+AGG 0.698278 1.4:+83715604 None:intergenic
ATAACGGTGTTGAAACGGAG+AGG 0.717587 1.4:-83715430 MS.gene73436:CDS
CCACCCACGCTACAAAACTG+AGG 0.721664 1.4:-83713180 MS.gene73436:CDS
AGATGGTTGTGTACAACCAG+GGG 0.741581 1.4:-83713310 MS.gene73436:CDS
GTACAACGAAGCAAACACGC+CGG 0.782658 1.4:+83715925 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTATGTTGAAAATA+AGG - chr1.4:83715362-83715381 MS.gene73436:CDS 10.0%
!! AAAGCATATATTAATAATAG+AGG + chr1.4:83715341-83715360 None:intergenic 15.0%
!! ATTATAATGTAAAAACTGTA+TGG + chr1.4:83714819-83714838 None:intergenic 15.0%
!! TAACAAAACAAATAACTAAA+TGG + chr1.4:83715246-83715265 None:intergenic 15.0%
!! TAACATCAATAATAATAACA+AGG - chr1.4:83713558-83713577 MS.gene73436:intron 15.0%
!!! CAGTTTTTACATTATAATTA+AGG - chr1.4:83714821-83714840 MS.gene73436:intron 15.0%
!!! TTTTTACATTATAATTAAGG+TGG - chr1.4:83714824-83714843 MS.gene73436:intron 15.0%
!! TAGATTTGATTGTTGATATT+CGG - chr1.4:83715641-83715660 MS.gene73436:CDS 20.0%
!!! AATTGTGTTCAATTTTGAAA+TGG - chr1.4:83715420-83715439 MS.gene73436:CDS 20.0%
!!! CGATATTTTAAACTAATAGA+TGG - chr1.4:83714558-83714577 MS.gene73436:intron 20.0%
!!! GCATCAAAATTTAACTTAAT+TGG + chr1.4:83714794-83714813 None:intergenic 20.0%
!!! TGGTTTTTCTAAGTTTTAAA+GGG - chr1.4:83715078-83715097 MS.gene73436:intron 20.0%
!!! TTGGTTTTTCTAAGTTTTAA+AGG - chr1.4:83715077-83715096 MS.gene73436:intron 20.0%
!!! TTTAAAACTTAGAAAAACCA+AGG + chr1.4:83715078-83715097 None:intergenic 20.0%
! TCATACAATGTCTACATTAT+AGG - chr1.4:83714925-83714944 MS.gene73436:intron 25.0%
! TTACATTATTATGAACGTCA+AGG + chr1.4:83715571-83715590 None:intergenic 25.0%
!!! AAAAACCAAGGTATTGATTT+TGG + chr1.4:83715066-83715085 None:intergenic 25.0%
!!! ACATTATAGGAGTATTTAAG+AGG - chr1.4:83714938-83714957 MS.gene73436:intron 25.0%
!!! ATGAGATAAAGATTTTAGAG+AGG - chr1.4:83714586-83714605 MS.gene73436:intron 25.0%
!!! GGTTTTTCTAAGTTTTAAAG+GGG - chr1.4:83715079-83715098 MS.gene73436:intron 25.0%
!!! TAAAGATTTTAGAGAGGAAA+CGG - chr1.4:83714592-83714611 MS.gene73436:intron 25.0%
!!! TAAGTTTTAAAGGGGATAAA+TGG - chr1.4:83715087-83715106 MS.gene73436:intron 25.0%
AAACATTAGTATGACTGTCA+AGG + chr1.4:83715475-83715494 None:intergenic 30.0%
AACCAACAACTAGTATAAAC+TGG + chr1.4:83714205-83714224 None:intergenic 30.0%
ACATTTATGATAGCCATGTA+TGG - chr1.4:83715000-83715019 MS.gene73436:intron 30.0%
ACTAACAAGCAAGTCAAATT+TGG + chr1.4:83714051-83714070 None:intergenic 30.0%
CTGTATATGGTTTCAAATTG+CGG - chr1.4:83714335-83714354 MS.gene73436:intron 30.0%
GAAAAAACAACCAAACTACA+AGG + chr1.4:83714145-83714164 None:intergenic 30.0%
GTAATGTCTTGAATCTTCAT+TGG - chr1.4:83715282-83715301 MS.gene73436:intron 30.0%
TAATAATAACAAGGAGTTGC+AGG - chr1.4:83713567-83713586 MS.gene73436:intron 30.0%
TACTTCGAGATAAAGATGAA+TGG - chr1.4:83715389-83715408 MS.gene73436:CDS 30.0%
TATATAGAACTAGCAAGACT+AGG - chr1.4:83714890-83714909 MS.gene73436:intron 30.0%
! AAGCAAGGTTTTACTTCATT+TGG + chr1.4:83714091-83714110 None:intergenic 30.0%
! AAGGTTTTACTTCATTTGGA+AGG + chr1.4:83714087-83714106 None:intergenic 30.0%
! GTCCACTCATTGTTATTAAT+CGG + chr1.4:83715615-83715634 None:intergenic 30.0%
! TGTCACTCAATTTCTTTTCA+TGG - chr1.4:83715156-83715175 MS.gene73436:intron 30.0%
!! TTTTAAAGGGGATAAATGGT+TGG - chr1.4:83715091-83715110 MS.gene73436:intron 30.0%
AAACCATATACAGAGCACAT+TGG + chr1.4:83714328-83714347 None:intergenic 35.0%
AAACCTTGCAACACATCAAA+CGG + chr1.4:83713484-83713503 None:intergenic 35.0%
ACGAGTGTGATTGAGAATAA+CGG - chr1.4:83713616-83713635 MS.gene73436:intron 35.0%
AGCATTTCTGTTAGATCCAA+TGG - chr1.4:83713670-83713689 MS.gene73436:intron 35.0%
ATGGCAAACTAGAGCTAATT+TGG + chr1.4:83714243-83714262 None:intergenic 35.0%
CAATAGCTGCAATATTGCAA+AGG - chr1.4:83714708-83714727 MS.gene73436:intron 35.0%
CAGGTTAAAATGTCACAGAA+AGG + chr1.4:83714296-83714315 None:intergenic 35.0%
CTCCAGTTTATACTAGTTGT+TGG - chr1.4:83714200-83714219 MS.gene73436:intron 35.0%
CTGTAACAAACCTTGTAGTT+TGG - chr1.4:83714132-83714151 MS.gene73436:intron 35.0%
GATCTAACAGAAATGCTCTT+TGG + chr1.4:83713667-83713686 None:intergenic 35.0%
TACAACTGTATCAGATTGAG+TGG + chr1.4:83713782-83713801 None:intergenic 35.0%
TACAAGTAACAAGTGATGCA+GGG - chr1.4:83715122-83715141 MS.gene73436:intron 35.0%
TGACATAGCTTTCTCTTGTT+CGG + chr1.4:83715977-83715996 None:intergenic 35.0%
TTACAAGTAACAAGTGATGC+AGG - chr1.4:83715121-83715140 MS.gene73436:intron 35.0%
TTAGTATGACTGTCAAGGAA+TGG + chr1.4:83715470-83715489 None:intergenic 35.0%
TTATGATCACACCACAATTG+CGG + chr1.4:83714407-83714426 None:intergenic 35.0%
TTATTATGAACGTCAAGGCA+TGG + chr1.4:83715566-83715585 None:intergenic 35.0%
TTATTCTCAATCACACTCGT+CGG + chr1.4:83713616-83713635 None:intergenic 35.0%
! AGCATTGTTGTTAGTTACGA+CGG + chr1.4:83713722-83713741 None:intergenic 35.0%
! CACCGATTAATAACAATGAG+TGG - chr1.4:83715610-83715629 MS.gene73436:CDS 35.0%
! TGAGAATAACGGTGTTGAAA+CGG - chr1.4:83713627-83713646 MS.gene73436:intron 35.0%
!! AAAATTAAGTTGACGCCACA+AGG - chr1.4:83714669-83714688 MS.gene73436:intron 35.0%
!!! ACAACCACGGTTTTAAAATG+CGG + chr1.4:83714752-83714771 None:intergenic 35.0%
AAATGGCATCTATGGCCATA+CGG + chr1.4:83715940-83715959 None:intergenic 40.0%
AACTGTATCAGATTGAGTGG+AGG + chr1.4:83713779-83713798 None:intergenic 40.0%
AATTGACAGTGGTCTCCATA+AGG + chr1.4:83715826-83715845 None:intergenic 40.0%
ACAAGTAACAAGTGATGCAG+GGG - chr1.4:83715123-83715142 MS.gene73436:intron 40.0%
AGACTGAGCTCTGTAACAAA+TGG - chr1.4:83715782-83715801 MS.gene73436:CDS 40.0%
AGTCGCCAAAATCAATACCT+TGG - chr1.4:83715058-83715077 MS.gene73436:intron 40.0%
ATGGAAATTTGCAGTGCTGT+TGG - chr1.4:83715019-83715038 MS.gene73436:intron 40.0%
ATTGACAGTGGTCTCCATAA+GGG + chr1.4:83715825-83715844 None:intergenic 40.0%
CTTCTGCAACTCTTTGTTCA+CGG + chr1.4:83713383-83713402 None:intergenic 40.0%
CTTGAATCTTGGAGATGGAA+AGG - chr1.4:83713510-83713529 MS.gene73436:intron 40.0%
CTTTATCCTTACTCTGACCT+TGG - chr1.4:83713226-83713245 MS.gene73436:CDS 40.0%
CTTTCCAGCTTCATTGATCT+GGG + chr1.4:83716009-83716028 None:intergenic 40.0%
GCACTGCAAATTTCCATACA+TGG + chr1.4:83715016-83715035 None:intergenic 40.0%
TCTTTCCAGCTTCATTGATC+TGG + chr1.4:83716010-83716029 None:intergenic 40.0%
TGTAACAAATGGCAGGAAAC+TGG - chr1.4:83715793-83715812 MS.gene73436:CDS 40.0%
TGTTACTATGCTGCAGCAAA+AGG - chr1.4:83715690-83715709 MS.gene73436:CDS 40.0%
TTGTACACAACCATCTCAAG+AGG + chr1.4:83715748-83715767 None:intergenic 40.0%
! GAAAGGAAAACTGTGCTGAT+TGG - chr1.4:83713527-83713546 MS.gene73436:intron 40.0%
! TTGTTACTCACTGCTGACTA+TGG - chr1.4:83715260-83715279 MS.gene73436:intron 40.0%
! TTGTTGGTTAGTAGTGTTGC+AGG - chr1.4:83714216-83714235 MS.gene73436:intron 40.0%
!! AAAAGGTATTTGTGCGAGGA+GGG - chr1.4:83715707-83715726 MS.gene73436:CDS 40.0%
!! ATTAGCTCTAGTTTGCCATG+AGG - chr1.4:83714244-83714263 MS.gene73436:intron 40.0%
!! CAACTTCTTTGGAACTTTCC+AGG + chr1.4:83713327-83713346 None:intergenic 40.0%
!! CAAGGTTTGTTACAGCTAAC+AGG + chr1.4:83714127-83714146 None:intergenic 40.0%
!! GAATCTTCATTGGTTAGCCT+TGG - chr1.4:83715292-83715311 MS.gene73436:CDS 40.0%
!! GATACAGAAGCATCAGGATA+TGG - chr1.4:83713270-83713289 MS.gene73436:CDS 40.0%
!!! ATGGTTTTATCCACTGCTGT+TGG - chr1.4:83715175-83715194 MS.gene73436:intron 40.0%
AAACCGTGGTTGTTTGTGCT+TGG - chr1.4:83714759-83714778 MS.gene73436:intron 45.0%
AAGGGGATAAATGGTTGGAC+TGG - chr1.4:83715096-83715115 MS.gene73436:intron 45.0%
AATGGCATCTATGGCCATAC+GGG + chr1.4:83715939-83715958 None:intergenic 45.0%
ACACCAAGCACAAACAACCA+CGG + chr1.4:83714765-83714784 None:intergenic 45.0%
ACTCCGTTTGATGTGTTGCA+AGG - chr1.4:83713478-83713497 MS.gene73436:intron 45.0%
AGATGGTTGTGTACAACCAG+GGG - chr1.4:83715752-83715771 MS.gene73436:CDS 45.0%
AGCAAGCTTCAAGTAGCCAT+TGG + chr1.4:83713689-83713708 None:intergenic 45.0%
AGTGCCGTAATGAGGGAATT+GGG - chr1.4:83714449-83714468 MS.gene73436:intron 45.0%
ATGACTGTCAAGGAATGGCA+AGG + chr1.4:83715465-83715484 None:intergenic 45.0%
ATGAGTCGTACTCTGTTCCA+AGG + chr1.4:83713246-83713265 None:intergenic 45.0%
CAAACGGAGTAGATTGAGTC+TGG + chr1.4:83713468-83713487 None:intergenic 45.0%
CATCTCCAAGATTCAAGCCA+CGG + chr1.4:83713507-83713526 None:intergenic 45.0%
CTGCAGAGCTTCAACTTCTT+TGG + chr1.4:83713338-83713357 None:intergenic 45.0%
CTTGGACACATCTTGTGCTT+GGG - chr1.4:83715310-83715329 MS.gene73436:CDS 45.0%
CTTGTTCGGTAAGTGCATGA+CGG + chr1.4:83715963-83715982 None:intergenic 45.0%
GAGATGGTTGTGTACAACCA+GGG - chr1.4:83715751-83715770 MS.gene73436:CDS 45.0%
GATGTGTATTGTCGTCCTTG+TGG + chr1.4:83714687-83714706 None:intergenic 45.0%
GGTAATGACACAGACAAGCA+AGG + chr1.4:83714106-83714125 None:intergenic 45.0%
GTCAATTCGCTCATGGCATT+GGG - chr1.4:83715839-83715858 MS.gene73436:CDS 45.0%
GTGCCAATGTGCTCTGTATA+TGG - chr1.4:83714322-83714341 MS.gene73436:intron 45.0%
TAAGCCCAGATCAATGAAGC+TGG - chr1.4:83716002-83716021 MS.gene73436:CDS 45.0%
TATGTTTCTCAGTCGTCTCC+TGG - chr1.4:83713306-83713325 MS.gene73436:CDS 45.0%
TCAATTCGCTCATGGCATTG+GGG - chr1.4:83715840-83715859 MS.gene73436:CDS 45.0%
TCTGTTCCAAGGTCAGAGTA+AGG + chr1.4:83713235-83713254 None:intergenic 45.0%
TGAGATGGTTGTGTACAACC+AGG - chr1.4:83715750-83715769 MS.gene73436:CDS 45.0%
TGAGCTCTGTAACAAATGGC+AGG - chr1.4:83715786-83715805 MS.gene73436:CDS 45.0%
TGCACCCAATTCCCTCATTA+CGG + chr1.4:83714456-83714475 None:intergenic 45.0%
TGTCAATTCGCTCATGGCAT+TGG - chr1.4:83715838-83715857 MS.gene73436:CDS 45.0%
TGTTCTCCGTCATCCAAAAG+CGG - chr1.4:83713070-83713089 MS.gene73436:CDS 45.0%
! AGCTCATCTGTTTTGCCTCA+TGG + chr1.4:83714262-83714281 None:intergenic 45.0%
! ATAACGGTGTTGAAACGGAG+AGG - chr1.4:83713632-83713651 MS.gene73436:intron 45.0%
! ATTTGCAGTGCTGTTGGTAG+TGG - chr1.4:83715025-83715044 MS.gene73436:intron 45.0%
! CATGACGGAAATGGCATCTA+TGG + chr1.4:83715948-83715967 None:intergenic 45.0%
! TTGTTGTTAGTTACGACGGC+CGG + chr1.4:83713718-83713737 None:intergenic 45.0%
!! AGACCTCAGTTTTGTAGCGT+GGG + chr1.4:83715888-83715907 None:intergenic 45.0%
!! ATGCGTGATACAGAAGCATC+AGG - chr1.4:83713264-83713283 MS.gene73436:CDS 45.0%
!! CAAAAGGTATTTGTGCGAGG+AGG - chr1.4:83715706-83715725 MS.gene73436:CDS 45.0%
!! CAGCAAAAGGTATTTGTGCG+AGG - chr1.4:83715703-83715722 MS.gene73436:CDS 45.0%
!!! ATTTACTATTATTTATTTTA+TGG + chr1.4:83714630-83714649 None:intergenic 5.0%
AACAATGCTTGCCGCACCAA+AGG - chr1.4:83713733-83713752 MS.gene73436:intron 50.0%
AGAAGTTGAAGCTCTGCAGC+AGG - chr1.4:83713339-83713358 MS.gene73436:CDS 50.0%
AGAGGAAGAGCCTCTTGAGA+TGG - chr1.4:83715735-83715754 MS.gene73436:CDS 50.0%
AGCACATTGGCACAGAACAC+AGG + chr1.4:83714315-83714334 None:intergenic 50.0%
AGGATCCGCCACCATTGAAT+CGG + chr1.4:83713441-83713460 None:intergenic 50.0%
AGTAGATTGAGTCTGGACGG+AGG + chr1.4:83713461-83713480 None:intergenic 50.0%
ATCGCATCAAGCCGCAATTG+TGG - chr1.4:83714393-83714412 MS.gene73436:intron 50.0%
CAAAACTGAGGTCTGCAGGA+TGG - chr1.4:83715894-83715913 MS.gene73436:CDS 50.0%
CAGTGCCGTAATGAGGGAAT+TGG - chr1.4:83714448-83714467 MS.gene73436:intron 50.0%
CCAAGCACAAGATGTGTCCA+AGG + chr1.4:83715312-83715331 None:intergenic 50.0%
CCGTCATCCAAAAGCGGTTA+CGG - chr1.4:83713076-83713095 MS.gene73436:CDS 50.0%
CCTTGGACACATCTTGTGCT+TGG - chr1.4:83715309-83715328 MS.gene73436:CDS 50.0%
CGGAGTAGATTGAGTCTGGA+CGG + chr1.4:83713464-83713483 None:intergenic 50.0%
CGTCGGACTCTCATCAGATT+CGG + chr1.4:83713599-83713618 None:intergenic 50.0%
CGTGGCTTGAATCTTGGAGA+TGG - chr1.4:83713505-83713524 MS.gene73436:intron 50.0%
GAAACTGGCACATGCCCTTA+TGG - chr1.4:83715808-83715827 MS.gene73436:CDS 50.0%
GAAAGCTACACCAACAGCAG+TGG + chr1.4:83715188-83715207 None:intergenic 50.0%
GAAGCTCTGCAGCAGGAAAA+CGG - chr1.4:83713346-83713365 MS.gene73436:CDS 50.0%
GACCACTGTCAATTCGCTCA+TGG - chr1.4:83715832-83715851 MS.gene73436:CDS 50.0%
GATGTGTTGCAAGGTTTCCG+TGG - chr1.4:83713487-83713506 MS.gene73436:intron 50.0%
GCAACTCTTTGTTCACGGCA+CGG + chr1.4:83713378-83713397 None:intergenic 50.0%
GCGATTGCAGACAATACCGT+GGG + chr1.4:83714378-83714397 None:intergenic 50.0%
GCTACAAAACTGAGGTCTGC+AGG - chr1.4:83715890-83715909 MS.gene73436:CDS 50.0%
GGTTTCCGTGGCTTGAATCT+TGG - chr1.4:83713499-83713518 MS.gene73436:intron 50.0%
GTACAACGAAGCAAACACGC+CGG + chr1.4:83713140-83713159 None:intergenic 50.0%
TCCAAAAGCGGTTACGGCAT+CGG - chr1.4:83713082-83713101 MS.gene73436:CDS 50.0%
TGCCATGAGCGAATTGACAG+TGG + chr1.4:83715837-83715856 None:intergenic 50.0%
TGCGATTGCAGACAATACCG+TGG + chr1.4:83714379-83714398 None:intergenic 50.0%
! CGGTAAGTGCATGACGGAAA+TGG + chr1.4:83715957-83715976 None:intergenic 50.0%
! CTGGAGAACCGATTCAATGG+TGG - chr1.4:83713430-83713449 MS.gene73436:intron 50.0%
! GAGAACCGATTCAATGGTGG+CGG - chr1.4:83713433-83713452 MS.gene73436:intron 50.0%
! TCGCTGGAGAACCGATTCAA+TGG - chr1.4:83713427-83713446 MS.gene73436:intron 50.0%
!! CAGACCTCAGTTTTGTAGCG+TGG + chr1.4:83715889-83715908 None:intergenic 50.0%
!! CCGTAACCGCTTTTGGATGA+CGG + chr1.4:83713079-83713098 None:intergenic 50.0%
AAAGGCAGCGACTGCAACAG+CGG - chr1.4:83714726-83714745 MS.gene73436:intron 55.0%
ACGTCGCAGTGCCGTAATGA+GGG - chr1.4:83714442-83714461 MS.gene73436:intron 55.0%
CAGATTGAGTGGAGGAAGCG+CGG + chr1.4:83713771-83713790 None:intergenic 55.0%
CCACCCACGCTACAAAACTG+AGG - chr1.4:83715882-83715901 MS.gene73436:CDS 55.0%
CCAGCGAAGCTTGAACGAGA+AGG + chr1.4:83713414-83713433 None:intergenic 55.0%
CCTTCTCGTTCAAGCTTCGC+TGG - chr1.4:83713411-83713430 MS.gene73436:intron 55.0%
CTGTCAAGGAATGGCAAGGC+AGG + chr1.4:83715461-83715480 None:intergenic 55.0%
GATCTTCCATCATCGCTCGC+CGG - chr1.4:83713175-83713194 MS.gene73436:CDS 55.0%
GCTCAGTCTTGAACATCCCC+TGG + chr1.4:83715771-83715790 None:intergenic 55.0%
TCTGCAGGATGGTCCTTGCT+GGG - chr1.4:83715905-83715924 MS.gene73436:CDS 55.0%
TTGTTAGTTACGACGGCCGG+AGG + chr1.4:83713715-83713734 None:intergenic 55.0%
! AGATTGAGTGGAGGAAGCGC+GGG + chr1.4:83713770-83713789 None:intergenic 55.0%
!! CAGTTTTGTAGCGTGGGTGG+CGG + chr1.4:83715882-83715901 None:intergenic 55.0%
!! CCTCAGTTTTGTAGCGTGGG+TGG + chr1.4:83715885-83715904 None:intergenic 55.0%
!!! GCGGCCGCATTTTAAAACCG+TGG - chr1.4:83714745-83714764 MS.gene73436:intron 55.0%
ACGGGCATACAACCCCAGCA+AGG + chr1.4:83715921-83715940 None:intergenic 60.0%
AGCGAGTAGCGCCTTTGGTG+CGG + chr1.4:83713747-83713766 None:intergenic 60.0%
CACGTCGCAGTGCCGTAATG+AGG - chr1.4:83714441-83714460 MS.gene73436:intron 60.0%
CTCATTACGGCACTGCGACG+TGG + chr1.4:83714443-83714462 None:intergenic 60.0%
CTCGCCGGAAACAGCAGCAA+CGG - chr1.4:83713190-83713209 MS.gene73436:CDS 60.0%
CTGCAGGATGGTCCTTGCTG+GGG - chr1.4:83715906-83715925 MS.gene73436:CDS 60.0%
CTGTTTCCGGCGAGCGATGA+TGG + chr1.4:83713184-83713203 None:intergenic 60.0%
GACAATACCGTGGGCGCATC+AGG + chr1.4:83714369-83714388 None:intergenic 60.0%
GCTGGGGTTGTATGCCCGTA+TGG - chr1.4:83715922-83715941 MS.gene73436:CDS 60.0%
GGCGTTCGACTGCATGTGAC+CGG + chr1.4:83713119-83713138 None:intergenic 60.0%
GGTCACATGCAGTCGAACGC+CGG - chr1.4:83713118-83713137 MS.gene73436:CDS 60.0%
GTCTGCAGGATGGTCCTTGC+TGG - chr1.4:83715904-83715923 MS.gene73436:CDS 60.0%
TGCATCACCTGATGCGCCCA+CGG - chr1.4:83714359-83714378 MS.gene73436:intron 60.0%
TGTGCGAGGAGGGCAGAAAG+AGG - chr1.4:83715717-83715736 MS.gene73436:CDS 60.0%
TTAGTTACGACGGCCGGAGG+CGG + chr1.4:83713712-83713731 None:intergenic 60.0%
! CTTGAAGCTTGCTCCGCCTC+CGG - chr1.4:83713696-83713715 MS.gene73436:intron 60.0%
! GCCGATGCCGTAACCGCTTT+TGG + chr1.4:83713086-83713105 None:intergenic 60.0%
GCCGGAAACAGCAGCAACGG+CGG - chr1.4:83713193-83713212 MS.gene73436:CDS 65.0%
GGAAACAGCAGCAACGGCGG+CGG - chr1.4:83713196-83713215 MS.gene73436:CDS 65.0%
!! AGCGTGGGTGGCGGATCACT+GGG + chr1.4:83715873-83715892 None:intergenic 65.0%
!! GCCGCCGTTGCTGCTGTTTC+CGG + chr1.4:83713197-83713216 None:intergenic 65.0%
!! GGCATCGGCGTCAACAACGC+CGG - chr1.4:83713097-83713116 MS.gene73436:CDS 65.0%
!! TAGCGTGGGTGGCGGATCAC+TGG + chr1.4:83715874-83715893 None:intergenic 65.0%
GCGGGAGCGAGTAGCGCCTT+TGG + chr1.4:83713752-83713771 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 83713052 83716032 83713052 ID=MS.gene73436
chr1.4 mRNA 83713052 83716032 83713052 ID=MS.gene73436.t1;Parent=MS.gene73436
chr1.4 exon 83715286 83716032 83715286 ID=MS.gene73436.t1.exon1;Parent=MS.gene73436.t1
chr1.4 CDS 83715286 83716032 83715286 ID=cds.MS.gene73436.t1;Parent=MS.gene73436.t1
chr1.4 exon 83713052 83713378 83713052 ID=MS.gene73436.t1.exon2;Parent=MS.gene73436.t1
chr1.4 CDS 83713052 83713378 83713052 ID=cds.MS.gene73436.t1;Parent=MS.gene73436.t1
Gene Sequence

>MS.gene73436

ATGCATAACAAGGACGTTTGTTCTCCGTCATCCAAAAGCGGTTACGGCATCGGCGTCAACAACGCCGGTCACATGCAGTCGAACGCCGGCGTGTTTGCTTCGTTGTACTCTGCTCTGAGTAATGATCTTCCATCATCGCTCGCCGGAAACAGCAGCAACGGCGGCGGATTTTCGCTTTATCCTTACTCTGACCTTGGAACAGAGTACGACTCATGCGTGATACAGAAGCATCAGGATATGGTGAATCGTCACAGTATGTTTCTCAGTCGTCTCCTGGAAAGTTCCAAAGAAGTTGAAGCTCTGCAGCAGGAAAACGGACAGCTCCGTGCCGTGAACAAAGAGTTGCAGAAGAATCTGAACCTTCTCGTTCAAGCTTCGCTGGAGAACCGATTCAATGGTGGCGGATCCTCCGTCCAGACTCAATCTACTCCGTTTGATGTGTTGCAAGGTTTCCGTGGCTTGAATCTTGGAGATGGAAAGGAAAACTGTGCTGATTGGAATAGCAATAACATCAATAATAATAACAAGGAGTTGCAGGAAGCTTCCGAATCTGATGAGAGTCCGACGAGTGTGATTGAGAATAACGGTGTTGAAACGGAGAGGTTCTCGCTTCCAAAGAGCATTTCTGTTAGATCCAATGGCTACTTGAAGCTTGCTCCGCCTCCGGCCGTCGTAACTAACAACAATGCTTGCCGCACCAAAGGCGCTACTCGCTCCCGCGCTTCCTCCACTCAATCTGATACAGTTGTAAGCACACGCACGTTATGATTGCTTTTATTTTATTTAATTAATTAATTAATTAATTTTAATTATAATTATAATTATAATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTATTTTATTTTATTTTATTATTATTGCTGCTATTGTTATTGTGATCTTAGCTTAATTTAATGTGTAATTATTGCTTTTATAGTTATAAACCAAATTTGACTTGCTTGTTAGTAGCTAATTATTTTCCTTCCAAATGAAGTAAAACCTTGCTTGTCTGTGTCATTACCTGTTAGCTGTAACAAACCTTGTAGTTTGGTTGTTTTTTCATTTGCGTGTGCTTTTTGCAAAGATGCAAGTTCGACTCCAGTTTATACTAGTTGTTGGTTAGTAGTGTTGCAGGACCAAATTAGCTCTAGTTTGCCATGAGGCAAAACAGATGAGCTAAACTTTGAAGCCTTTCTGTGACATTTTAACCTGTGTTCTGTGCCAATGTGCTCTGTATATGGTTTCAAATTGCGGATGCATCACCTGATGCGCCCACGGTATTGTCTGCAATCGCATCAAGCCGCAATTGTGGTGTGATCATAAATCACATTTGAAACCACGTCGCAGTGCCGTAATGAGGGAATTGGGTGCAGAAAACTAGTGATCACACAATTTATTTTTATACTTTACTTTATCTTTATATCATAGATTTAGAGAGTATTTTAAAGAACTAGCGATATTTTAAACTAATAGATGGCTGATATGAGATAAAGATTTTAGAGAGGAAACGGAAGATACATGAGCCATAAAATAAATAATAGTAAATTATAGTATTTATGCTAATAAAAATTAAGTTGACGCCACAAGGACGACAATACACATCGCAATAGCTGCAATATTGCAAAGGCAGCGACTGCAACAGCGGCCGCATTTTAAAACCGTGGTTGTTTGTGCTTGGTGTCATGTTCCAATTAAGTTAAATTTTGATGCAGCCATACAGTTTTTACATTATAATTAAGGTGGTTAAATAATTTTTCTCACACTTCTCAATAAACTCTTGAAATAATATATAGAACTAGCAAGACTAGGAAGATTGATGAGTCATACAATGTCTACATTATAGGAGTATTTAAGAGGTCAAATATATACTATTTTTCTACATTTCTGAATTTTAAGACATTTATGATAGCCATGTATGGAAATTTGCAGTGCTGTTGGTAGTGGTTTGTATTTTAGTCGCCAAAATCAATACCTTGGTTTTTCTAAGTTTTAAAGGGGATAAATGGTTGGACTGGTTTTACAAGTAACAAGTGATGCAGGGGATGCAGAATATGTCACTCAATTTCTTTTCATGGTTTTATCCACTGCTGTTGGTGTAGCTTTCAAATAGACGTATAGTGTGCTGCTTATAGTTTGTCTCCATTTAGTTATTTGTTTTGTTACTCACTGCTGACTATGGTAATGTCTTGAATCTTCATTGGTTAGCCTTGGACACATCTTGTGCTTGGGATTAGCCTCTATTATTAATATATGCTTTGATTTATTTATGTTGAAAATAAGGACAATACTTCGAGATAAAGATGAATGGATGTAAATAATTGTGTTCAATTTTGAAATGGTGTTTTCTTGAATGTCCTGCCTTGCCATTCCTTGACAGTCATACTAATGTTTCTTTCATCTTGACTGATTGTATTTTTCATCTTATTGTTTTTTTTATTTGATTCATATTCAATGCTCTGCCATGCCTTGACGTTCATAATAATGTAAAAGTAGATTGTAGATGCATCACCGATTAATAACAATGAGTGGACATTATATAGATTTGATTGTTGATATTCGGAAGAATTGATCATTGTTGAAATTTTGTGTTACTATGCTGCAGCAAAAGGTATTTGTGCGAGGAGGGCAGAAAGAGGAAGAGCCTCTTGAGATGGTTGTGTACAACCAGGGGATGTTCAAGACTGAGCTCTGTAACAAATGGCAGGAAACTGGCACATGCCCTTATGGAGACCACTGTCAATTCGCTCATGGCATTGGGGAGCTTCGCCCAGTGATCCGCCACCCACGCTACAAAACTGAGGTCTGCAGGATGGTCCTTGCTGGGGTTGTATGCCCGTATGGCCATAGATGCCATTTCCGTCATGCACTTACCGAACAAGAGAAAGCTATGTCACAATCTAAGCCCAGATCAATGAAGCTGGAAAGATGA
Protein sequence

>MS.gene73436.t1

MHNKDVCSPSSKSGYGIGVNNAGHMQSNAGVFASLYSALSNDLPSSLAGNSSNGGGFSLYPYSDLGTEYDSCVIQKHQDMVNRHSMFLSRLLESSKEVEALQQENGQLRAVNKELQKNLNLLVQASLENRFNGGGSSVQTQSTPFDVLQGFRGLNLGDGKENCADWNSNNINNNNKELQEASESDESPTSVIENNGVETERFSLPKSISVRSNGYLKLAPPPAVVTNNNACRTKGATRSRASSTQSDTVQKVFVRGGQKEEEPLEMVVYNQGMFKTELCNKWQETGTCPYGDHCQFAHGIGELRPVIRHPRYKTEVCRMVLAGVVCPYGHRCHFRHALTEQEKAMSQSKPRSMKLER