AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73457

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73457.t1 MTR_1g108780 95.556 450 20 0 33 482 12 461 0.0 907
MS.gene73457.t1 MTR_8g103940 69.792 480 136 6 1 480 26 496 0.0 683
MS.gene73457.t1 MTR_1g035460 46.305 406 194 11 34 427 2 395 3.61e-105 319
MS.gene73457.t1 MTR_3g098790 35.370 311 170 7 13 311 8 299 2.18e-53 182
MS.gene73457.t1 MTR_7g050710 35.556 315 166 10 16 311 11 307 2.89e-53 182
MS.gene73457.t1 MTR_7g050720 35.625 320 166 12 18 311 10 315 1.94e-51 177
MS.gene73457.t1 MTR_7g050580 34.494 316 147 9 18 311 13 290 7.22e-49 170
MS.gene73457.t1 MTR_7g050590 36.149 296 154 11 34 311 2 280 7.25e-49 170
MS.gene73457.t1 MTR_7g050560 32.915 319 164 8 10 311 5 290 1.27e-48 169
MS.gene73457.t1 MTR_3g061370 33.439 314 157 8 13 310 4 281 3.65e-48 167
MS.gene73457.t1 MTR_3g061350 33.654 312 153 10 16 310 10 284 6.09e-46 162
MS.gene73457.t1 MTR_3g061340 32.573 307 161 10 12 310 6 274 1.19e-45 160
MS.gene73457.t1 MTR_3g061340 34.884 129 76 4 351 478 153 274 1.87e-12 68.2
MS.gene73457.t1 MTR_3g061310 32.258 310 161 9 16 310 10 285 4.28e-44 157
MS.gene73457.t1 MTR_3g061310 29.927 274 153 11 234 478 22 285 1.37e-16 80.5
MS.gene73457.t1 MTR_5g044670 28.994 338 189 9 3 318 2 310 1.41e-43 157
MS.gene73457.t1 MTR_3g061320 32.258 310 155 11 16 310 10 279 3.55e-42 151
MS.gene73457.t1 MTR_3g061320 28.636 220 131 7 277 478 68 279 1.09e-11 65.9
MS.gene73457.t1 MTR_3g061330 31.769 277 139 8 16 277 10 251 2.50e-38 140
MS.gene73457.t1 MTR_6g061070 25.595 504 315 14 1 478 1 470 2.61e-35 137
MS.gene73457.t1 MTR_3g061290 40.667 150 80 2 16 158 10 157 2.24e-32 122
MS.gene73457.t1 MTR_4g119990 27.726 321 200 12 13 315 10 316 1.57e-31 125
MS.gene73457.t1 MTR_1g035570 31.373 306 165 10 27 315 14 291 2.82e-31 125
MS.gene73457.t1 MTR_6g043890 26.552 290 194 8 14 295 11 289 2.88e-29 118
MS.gene73457.t1 MTR_1g035620 25.105 474 271 19 18 479 9 410 2.80e-28 116
MS.gene73457.t1 MTR_3g005420 27.273 319 166 11 6 312 273 537 1.02e-27 116
MS.gene73457.t1 MTR_3g005420 31.000 100 68 1 18 116 40 139 5.53e-11 65.1
MS.gene73457.t1 MTR_5g070440 26.144 306 158 9 18 312 21 269 3.43e-26 107
MS.gene73457.t1 MTR_1g035640 27.976 336 201 11 27 355 14 315 3.45e-26 111
MS.gene73457.t1 MTR_7g050640 45.833 96 52 0 16 111 11 106 4.87e-26 102
MS.gene73457.t1 MTR_3g005480 25.329 304 155 8 18 312 21 261 7.70e-25 103
MS.gene73457.t1 MTR_3g061220 39.024 123 54 3 16 132 10 117 2.32e-21 89.7
MS.gene73457.t1 MTR_3g005460 24.204 314 168 8 15 312 259 518 4.06e-20 93.6
MS.gene73457.t1 MTR_3g005460 22.027 513 327 17 18 478 25 516 2.78e-19 90.9
MS.gene73457.t1 MTR_1g021400 25.378 331 197 12 23 315 36 354 4.23e-20 92.0
MS.gene73457.t1 MTR_1g021330 25.905 359 213 13 16 329 24 374 1.19e-19 90.9
MS.gene73457.t1 MTR_1g050845 25.974 308 161 9 18 309 9 265 1.89e-19 92.0
MS.gene73457.t1 MTR_4g068320 37.963 108 67 0 18 125 6 113 2.45e-19 90.5
MS.gene73457.t1 MTR_4g068320 38.931 131 72 4 199 322 270 399 6.45e-18 86.3
MS.gene73457.t1 MTR_1g035520 24.917 301 195 8 34 309 88 382 2.67e-19 90.1
MS.gene73457.t1 MTR_1g035490 25.952 289 170 9 202 482 79 331 7.23e-19 87.8
MS.gene73457.t1 MTR_1g021435 25.074 339 200 13 23 315 28 358 1.52e-18 87.4
MS.gene73457.t1 MTR_1g021360 25.887 282 176 8 32 299 196 458 1.44e-17 85.5
MS.gene73457.t1 MTR_1g034240 35.036 137 74 3 18 139 7 143 4.60e-17 83.6
MS.gene73457.t1 MTR_1g034240 36.434 129 72 5 199 319 274 400 5.23e-15 77.4
MS.gene73457.t1 MTR_3g005490 26.966 178 114 3 18 179 21 198 6.61e-17 80.9
MS.gene73457.t1 MTR_1g021380 22.687 335 204 11 16 315 20 334 7.85e-17 82.0
MS.gene73457.t1 MTR_1g098450 26.647 334 192 16 8 325 2 298 4.21e-16 79.7
MS.gene73457.t1 MTR_1g035650 35.000 100 63 1 17 116 8 105 9.40e-16 76.3
MS.gene73457.t1 MTR_1g033740 34.746 118 74 2 13 130 17 131 1.16e-15 75.9
MS.gene73457.t1 MTR_3g005495 25.685 292 141 9 18 302 243 465 1.92e-15 79.0
MS.gene73457.t1 MTR_3g005495 33.333 84 56 0 33 116 28 111 2.76e-12 68.9
MS.gene73457.t1 MTR_3g061300 37.288 118 69 3 366 482 14 127 1.56e-14 70.5
MS.gene73457.t1 MTR_3g061300 31.818 110 61 5 213 313 22 126 6.09e-11 60.5
MS.gene73457.t1 MTR_1g035500 33.898 118 75 2 13 130 20 134 3.56e-14 71.2
MS.gene73457.t1 MTR_1g035480 33.613 119 76 2 12 130 11 126 3.60e-14 71.2
MS.gene73457.t1 MTR_1g034210 34.746 118 68 4 209 319 251 366 6.61e-14 73.6
MS.gene73457.t1 MTR_1g034210 35.354 99 62 1 18 116 7 103 4.21e-12 68.2
MS.gene73457.t1 MTR_1g035440 33.010 103 68 1 15 116 12 114 2.62e-13 70.1
MS.gene73457.t1 MTR_1g035470 42.169 83 45 2 34 116 2 81 1.28e-12 65.5
MS.gene73457.t1 MTR_1g021290 33.333 129 72 3 19 134 157 284 2.31e-12 68.6
MS.gene73457.t1 MTR_1g034210 35.354 99 62 1 18 116 7 103 2.97e-12 68.2
MS.gene73457.t1 MTR_3g005440 31.765 85 58 0 32 116 34 118 3.28e-11 65.5
MS.gene73457.t1 MTR_3g005440 29.221 154 100 3 10 159 230 378 3.34e-11 65.5
MS.gene73457.t1 MTR_3g005420 31.000 100 68 1 18 116 21 120 5.40e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73457.t1 AT3G18990 32.344 337 185 11 18 314 6 339 1.61e-42 154
MS.gene73457.t1 AT3G18990 32.047 337 186 11 18 314 6 339 1.14e-41 152
MS.gene73457.t1 AT1G49480 31.977 172 88 7 169 314 56 224 7.16e-17 80.1
MS.gene73457.t1 AT1G49480 31.977 172 88 7 169 314 56 224 7.16e-17 80.1
MS.gene73457.t1 AT1G49480 31.977 172 88 7 169 314 56 224 7.16e-17 80.1
MS.gene73457.t1 AT4G01580 36.538 104 66 0 17 120 29 132 9.43e-17 79.0
MS.gene73457.t1 AT3G18960 34.615 104 68 0 17 120 29 132 5.96e-16 76.6
MS.gene73457.t1 AT3G18960 34.615 104 68 0 17 120 29 132 9.67e-16 76.6
MS.gene73457.t1 AT4G33280 26.333 300 198 10 32 318 37 326 9.70e-16 79.0
MS.gene73457.t1 AT1G49475 31.667 120 77 2 17 136 33 147 2.16e-14 72.0
MS.gene73457.t1 AT3G18960 35.227 88 57 0 33 120 8 95 1.72e-11 63.2

Find 76 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAATCCAATTGAAATTT+TGG 0.104888 1.4:+83424392 MS.gene73457:CDS
CCTAAACTTGTCCAAGTTAA+AGG 0.118978 1.4:+83424537 MS.gene73457:CDS
ATCTGATAATAATTTGAAAT+TGG 0.143719 1.4:+83425086 MS.gene73457:CDS
CTCAAATTGATAGTGAAATT+TGG 0.167485 1.4:-83425760 None:intergenic
GAACTTGAAATTTGTGATTT+TGG 0.194976 1.4:-83424444 None:intergenic
GTGAGACTGTACATATTCTT+AGG 0.199034 1.4:-83423697 None:intergenic
GGAAATACATTTGACTAAAA+AGG 0.231817 1.4:+83424194 MS.gene73457:CDS
CTTGACCATGCTTCACATTC+AGG 0.239372 1.4:-83424690 None:intergenic
TATCATGACAGCAGTGAAAA+AGG 0.253877 1.4:-83424782 None:intergenic
TTCTATTGATCATCTCAAAA+AGG 0.267399 1.4:-83425119 None:intergenic
CCTTCACCCAACTTTGTTGC+TGG 0.270614 1.4:-83426081 None:intergenic
CCTTTAACTTGGACAAGTTT+AGG 0.279816 1.4:-83424537 None:intergenic
CGAAATCGCCCTTCAGGTCT+TGG 0.340066 1.4:+83424996 MS.gene73457:CDS
TCAATTTGAGATCAAGTCAT+TGG 0.345291 1.4:+83425773 MS.gene73457:CDS
TGCTCATCCAAAATTTCAAT+TGG 0.347442 1.4:-83424399 None:intergenic
TATTTCTCAGTGAAAGCATT+TGG 0.349857 1.4:-83424117 None:intergenic
TCTGATAATAATTTGAAATT+GGG 0.355411 1.4:+83425087 MS.gene73457:CDS
GTACATATTCTTAGGGTTGC+TGG 0.358212 1.4:-83423689 None:intergenic
GACTGCAATGTTACAGTTTG+AGG 0.362858 1.4:+83426017 MS.gene73457:CDS
TTCATTCTCGAGCAAGTACT+TGG 0.373540 1.4:+83425981 MS.gene73457:CDS
AAAACTATTATGTCGTATAA+AGG 0.388718 1.4:+83425351 MS.gene73457:CDS
GCAAAGCGAAATCGCCCTTC+AGG 0.399129 1.4:+83424990 MS.gene73457:CDS
GAAATTGAATATCGTGTTGA+CGG 0.403003 1.4:+83424351 MS.gene73457:CDS
TGTAGAAGAAAATAAATTGC+AGG 0.404615 1.4:+83426116 MS.gene73457:CDS
AAGCACAACATCTTCCTTGT+TGG 0.409928 1.4:-83426166 None:intergenic
CAAAGTGGATGTGAATTGAT+TGG 0.417163 1.4:-83425731 None:intergenic
CTTCACATTCAGGCATTTAG+TGG 0.427188 1.4:-83424680 None:intergenic
ATATTTGGATTCAAAGGGGT+TGG 0.428451 1.4:+83424223 MS.gene73457:CDS
ATTTCCCATTCTTTCTCATC+GGG 0.433534 1.4:-83424177 None:intergenic
ATGGTGATATTTGGATTCAA+AGG 0.444319 1.4:+83424217 MS.gene73457:CDS
AATTTATTATACCTTTAACT+TGG 0.446187 1.4:-83424548 None:intergenic
AACTTTGTTGCTGGTTCGAA+TGG 0.449147 1.4:-83426072 None:intergenic
TTCGAACCAGCAACAAAGTT+GGG 0.458098 1.4:+83426075 MS.gene73457:CDS
TGGTGATATTTGGATTCAAA+GGG 0.458234 1.4:+83424218 MS.gene73457:CDS
GGTCTACTCTTCTTGTGAGA+AGG 0.460496 1.4:-83424423 None:intergenic
ATTCTTAGGGTTGCTGGTCA+TGG 0.460769 1.4:-83423683 None:intergenic
TTGGATTCAAAGGGGTTGGA+AGG 0.464926 1.4:+83424227 MS.gene73457:CDS
AGACTCCCGATGAGAAAGAA+TGG 0.468520 1.4:+83424172 MS.gene73457:CDS
CAATCAATTCACATCCACTT+TGG 0.477065 1.4:+83425732 MS.gene73457:CDS
TCTTGGATGATGAGAGAACT+TGG 0.477954 1.4:+83425013 MS.gene73457:CDS
TTTGAATCAGTTCATGGAGA+TGG 0.482390 1.4:+83425285 MS.gene73457:intron
ACACATTATTCATTAGATCA+TGG 0.484576 1.4:+83424258 MS.gene73457:CDS
TTCATGGAGATGGAATTAAC+TGG 0.486836 1.4:+83425295 MS.gene73457:CDS
TATTTCCCATTCTTTCTCAT+CGG 0.492052 1.4:-83424178 None:intergenic
GGATAATACATCACTTGTAC+AGG 0.496972 1.4:-83426051 None:intergenic
CAATTTGAGATCAAGTCATT+GGG 0.498692 1.4:+83425774 MS.gene73457:CDS
TGAGACTGTACATATTCTTA+GGG 0.500244 1.4:-83423696 None:intergenic
GACTCCCGATGAGAAAGAAT+GGG 0.507920 1.4:+83424173 MS.gene73457:CDS
TTGGATTACCTTGTGGTGGA+AGG 0.508508 1.4:-83425194 None:intergenic
ATTCGAACCAGCAACAAAGT+TGG 0.517520 1.4:+83426074 MS.gene73457:CDS
ACCTCAACAAATATTCAAGA+TGG 0.526205 1.4:+83423751 MS.gene73457:CDS
AATTAGTACTTTAAAAGCAT+AGG 0.530987 1.4:-83425145 None:intergenic
GCTTTCACTGAGAAATATAG+TGG 0.532968 1.4:+83424123 MS.gene73457:CDS
TCTCATCATCCAAGACCTGA+AGG 0.534348 1.4:-83425005 None:intergenic
CAACAAAGTTGGGTGAAGGT+TGG 0.542982 1.4:+83426085 MS.gene73457:CDS
TGGATTACCTTGTGGTGGAA+GGG 0.545050 1.4:-83425193 None:intergenic
GAAGAAAATAAATTGCAGGC+TGG 0.548366 1.4:+83426120 MS.gene73457:CDS
TCGGGAGTCTTGAGAAACAT+TGG 0.550403 1.4:-83424159 None:intergenic
AACTCAAAGAAATTCACTGC+AGG 0.555404 1.4:+83425666 MS.gene73457:CDS
ATTAACTGGTTAGAAACTGC+AGG 0.556059 1.4:+83425309 MS.gene73457:CDS
AAAGAAATTCACTGCAGGCT+AGG 0.559813 1.4:+83425671 MS.gene73457:CDS
TCCATCTTGAATATTTGTTG+AGG 0.562475 1.4:-83423752 None:intergenic
TTCATTAGATCATGGTCACA+TGG 0.571925 1.4:+83424266 MS.gene73457:CDS
CATCTTGGATTACCTTGTGG+TGG 0.585751 1.4:-83425198 None:intergenic
GGTGATATTTGGATTCAAAG+GGG 0.604343 1.4:+83424219 MS.gene73457:CDS
TTCATATGTTAATCAATACA+TGG 0.607228 1.4:+83424807 MS.gene73457:CDS
CTCATCATCCAAGACCTGAA+GGG 0.610171 1.4:-83425004 None:intergenic
ATTGACACAAGTGAAGATGT+CGG 0.614793 1.4:+83424489 MS.gene73457:CDS
TATGTAAACCTCAAAATGAG+AGG 0.618304 1.4:-83424310 None:intergenic
CCAGCAACAAAGTTGGGTGA+AGG 0.622086 1.4:+83426081 MS.gene73457:CDS
ACGGTAATAATCAACATGAA+CGG 0.624679 1.4:+83424370 MS.gene73457:CDS
TCATTCTCGAGCAAGTACTT+GGG 0.630141 1.4:+83425982 MS.gene73457:CDS
CAACATTCCCTTCCACCACA+AGG 0.632695 1.4:+83425186 MS.gene73457:CDS
TGAAGCATGGTCAAGAGCAG+AGG 0.641697 1.4:+83424698 MS.gene73457:CDS
TTGGCATACCTCAGGAACGA+AGG 0.641759 1.4:+83425642 MS.gene73457:intron
AAATGCCTGAATGTGAAGCA+TGG 0.702749 1.4:+83424685 MS.gene73457:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATTACTAATAACTTTA+AGG - chr1.4:83425231-83425250 None:intergenic 15.0%
!! AATTTATTATACCTTTAACT+TGG - chr1.4:83424551-83424570 None:intergenic 15.0%
!!! ACTTTTGTTCAAAATTTAAA+GGG - chr1.4:83424957-83424976 None:intergenic 15.0%
!!! ATCTGATAATAATTTGAAAT+TGG + chr1.4:83425086-83425105 MS.gene73457:CDS 15.0%
!!! TAAAGTTATTAGTAATCTTT+TGG + chr1.4:83425231-83425250 MS.gene73457:intron 15.0%
!!! TACTTTTGTTCAAAATTTAA+AGG - chr1.4:83424958-83424977 None:intergenic 15.0%
!!! TCTGATAATAATTTGAAATT+GGG + chr1.4:83425087-83425106 MS.gene73457:CDS 15.0%
!! AAAACTATTATGTCGTATAA+AGG + chr1.4:83425351-83425370 MS.gene73457:CDS 20.0%
!! AATCACTAGATATATGTATT+TGG - chr1.4:83425843-83425862 None:intergenic 20.0%
!! AGGTATAATAAATTCATGAT+TGG + chr1.4:83424557-83424576 MS.gene73457:intron 20.0%
!! ATCATACAGTACTTAAATTT+CGG - chr1.4:83423831-83423850 None:intergenic 20.0%
!! TTAAAAAATGGAAAGAAAGA+AGG - chr1.4:83423797-83423816 None:intergenic 20.0%
!! TTCATATGTTAATCAATACA+TGG + chr1.4:83424807-83424826 MS.gene73457:CDS 20.0%
!!! AATTAGTACTTTAAAAGCAT+AGG - chr1.4:83425148-83425167 None:intergenic 20.0%
!!! AATTGATTATATGCTTTTGT+TGG + chr1.4:83425623-83425642 MS.gene73457:intron 20.0%
!!! TAATACAAAATGCTGTTTAA+TGG + chr1.4:83425483-83425502 MS.gene73457:intron 20.0%
!!! TCAATTCTCAATTTTTTAAG+TGG - chr1.4:83424474-83424493 None:intergenic 20.0%
!!! TTTTCTTAGAAAGATACATA+TGG - chr1.4:83423945-83423964 None:intergenic 20.0%
!!! TTTTGAACAAAAGTATTTGA+AGG + chr1.4:83424963-83424982 MS.gene73457:CDS 20.0%
! ACACATTATTCATTAGATCA+TGG + chr1.4:83424258-83424277 MS.gene73457:CDS 25.0%
! ATACATTTGACTAAAAAGGA+TGG + chr1.4:83424198-83424217 MS.gene73457:CDS 25.0%
! ATTAAAAGTTCAAGTGAGTT+AGG - chr1.4:83423903-83423922 None:intergenic 25.0%
! CATAAATACATATTCCTTAC+TGG - chr1.4:83425811-83425830 None:intergenic 25.0%
! GGAAATACATTTGACTAAAA+AGG + chr1.4:83424194-83424213 MS.gene73457:CDS 25.0%
! GTCGTTCAAAAGTTAAAAAA+TGG - chr1.4:83423809-83423828 None:intergenic 25.0%
! TGTAGAAGAAAATAAATTGC+AGG + chr1.4:83426116-83426135 MS.gene73457:CDS 25.0%
! TGTATGAGAAAAATTAGTAG+CGG + chr1.4:83425048-83425067 MS.gene73457:CDS 25.0%
! TTCTATTGATCATCTCAAAA+AGG - chr1.4:83425122-83425141 None:intergenic 25.0%
!! GAACAATCCAATTGAAATTT+TGG + chr1.4:83424392-83424411 MS.gene73457:CDS 25.0%
!! GAACTTGAAATTTGTGATTT+TGG - chr1.4:83424447-83424466 None:intergenic 25.0%
!! GTTTCTTTTACAGTTAGTTA+GGG - chr1.4:83425936-83425955 None:intergenic 25.0%
!! TGTTTCTTTTACAGTTAGTT+AGG - chr1.4:83425937-83425956 None:intergenic 25.0%
!!! CTCAAATTGATAGTGAAATT+TGG - chr1.4:83425763-83425782 None:intergenic 25.0%
ACCTCAACAAATATTCAAGA+TGG + chr1.4:83423751-83423770 MS.gene73457:CDS 30.0%
ACGGTAATAATCAACATGAA+CGG + chr1.4:83424370-83424389 MS.gene73457:CDS 30.0%
ATGGTGATATTTGGATTCAA+AGG + chr1.4:83424217-83424236 MS.gene73457:CDS 30.0%
ATTCAACTTCAAATTCCCAA+AGG - chr1.4:83425404-83425423 None:intergenic 30.0%
CAAAATTTAAAGGGACATAC+TGG - chr1.4:83424948-83424967 None:intergenic 30.0%
CTAAAAAGGATGGTGATATT+TGG + chr1.4:83424208-83424227 MS.gene73457:CDS 30.0%
CTAGTGATTATGCTTAAAAC+TGG + chr1.4:83425854-83425873 MS.gene73457:intron 30.0%
GAAATTGAATATCGTGTTGA+CGG + chr1.4:83424351-83424370 MS.gene73457:CDS 30.0%
TACAGTTTGAGGAAAAATTG+TGG + chr1.4:83426028-83426047 MS.gene73457:CDS 30.0%
TATGTAAACCTCAAAATGAG+AGG - chr1.4:83424313-83424332 None:intergenic 30.0%
TATTTCCCATTCTTTCTCAT+CGG - chr1.4:83424181-83424200 None:intergenic 30.0%
TGAGACTGTACATATTCTTA+GGG - chr1.4:83423699-83423718 None:intergenic 30.0%
TGCTCATCCAAAATTTCAAT+TGG - chr1.4:83424402-83424421 None:intergenic 30.0%
TGGTGATATTTGGATTCAAA+GGG + chr1.4:83424218-83424237 MS.gene73457:CDS 30.0%
TTCAACTTCAAATTCCCAAA+GGG - chr1.4:83425403-83425422 None:intergenic 30.0%
! AACATTGCAGTCTTCTTTTT+GGG - chr1.4:83426009-83426028 None:intergenic 30.0%
! CAATTTGAGATCAAGTCATT+GGG + chr1.4:83425774-83425793 MS.gene73457:CDS 30.0%
! CTCTTCTGAGAAATTGAATA+TGG - chr1.4:83424650-83424669 None:intergenic 30.0%
! CTTAGCTTCAGAATTTTTGA+AGG - chr1.4:83425704-83425723 None:intergenic 30.0%
! CTTGATGTTCATATTTTCAG+AGG + chr1.4:83426186-83426205 MS.gene73457:CDS 30.0%
! CTTTAAGGAAAAAACCATCT+TGG - chr1.4:83425216-83425235 None:intergenic 30.0%
! TAACATTGCAGTCTTCTTTT+TGG - chr1.4:83426010-83426029 None:intergenic 30.0%
! TCAATTTGAGATCAAGTCAT+TGG + chr1.4:83425773-83425792 MS.gene73457:CDS 30.0%
! TCCATCTTGAATATTTGTTG+AGG - chr1.4:83423755-83423774 None:intergenic 30.0%
! TTTAGGAATGTTTTGCGATT+TGG - chr1.4:83424523-83424542 None:intergenic 30.0%
!! TATTTCTCAGTGAAAGCATT+TGG - chr1.4:83424120-83424139 None:intergenic 30.0%
!!! GAAATTTGGTTTTTCCAAAG+TGG - chr1.4:83425749-83425768 None:intergenic 30.0%
!!! GTTGTTTGTTATGTTTTACG+CGG + chr1.4:83423852-83423871 MS.gene73457:intron 30.0%
AACTCAAAGAAATTCACTGC+AGG + chr1.4:83425666-83425685 MS.gene73457:CDS 35.0%
AGAAGGAAAGAATGCTTACA+AGG - chr1.4:83423780-83423799 None:intergenic 35.0%
ATATTTGGATTCAAAGGGGT+TGG + chr1.4:83424223-83424242 MS.gene73457:CDS 35.0%
ATCAAGCAGTATTGTCAAGA+TGG + chr1.4:83425422-83425441 MS.gene73457:intron 35.0%
ATCATGACAGCAGTGAAAAA+GGG - chr1.4:83424784-83424803 None:intergenic 35.0%
ATTAACTGGTTAGAAACTGC+AGG + chr1.4:83425309-83425328 MS.gene73457:CDS 35.0%
ATTGACACAAGTGAAGATGT+CGG + chr1.4:83424489-83424508 MS.gene73457:CDS 35.0%
ATTTCCCATTCTTTCTCATC+GGG - chr1.4:83424180-83424199 None:intergenic 35.0%
CAAAGTGGATGTGAATTGAT+TGG - chr1.4:83425734-83425753 None:intergenic 35.0%
CAATCAATTCACATCCACTT+TGG + chr1.4:83425732-83425751 MS.gene73457:CDS 35.0%
CCTAAACTTGTCCAAGTTAA+AGG + chr1.4:83424537-83424556 MS.gene73457:CDS 35.0%
CGTGAGTCGTAAAAAAATAG+AGG - chr1.4:83425532-83425551 None:intergenic 35.0%
GAAGAAAATAAATTGCAGGC+TGG + chr1.4:83426120-83426139 MS.gene73457:CDS 35.0%
GAAGGAAAGAATGCTTACAA+GGG - chr1.4:83423779-83423798 None:intergenic 35.0%
GATTATGCTTAAAACTGGAG+TGG + chr1.4:83425859-83425878 MS.gene73457:intron 35.0%
GCTTTCACTGAGAAATATAG+TGG + chr1.4:83424123-83424142 MS.gene73457:CDS 35.0%
GGATAATACATCACTTGTAC+AGG - chr1.4:83426054-83426073 None:intergenic 35.0%
GGTGATATTTGGATTCAAAG+GGG + chr1.4:83424219-83424238 MS.gene73457:CDS 35.0%
GTGAGACTGTACATATTCTT+AGG - chr1.4:83423700-83423719 None:intergenic 35.0%
TATCATGACAGCAGTGAAAA+AGG - chr1.4:83424785-83424804 None:intergenic 35.0%
TCATCCTTTGAATCAGTTCA+TGG + chr1.4:83425279-83425298 MS.gene73457:intron 35.0%
TTCATGGAGATGGAATTAAC+TGG + chr1.4:83425295-83425314 MS.gene73457:CDS 35.0%
TTCATTAGATCATGGTCACA+TGG + chr1.4:83424266-83424285 MS.gene73457:CDS 35.0%
TTTGAATCAGTTCATGGAGA+TGG + chr1.4:83425285-83425304 MS.gene73457:intron 35.0%
! ATCTCCATGAACTGATTCAA+AGG - chr1.4:83425286-83425305 None:intergenic 35.0%
! TCAGAAAACCTCTCATTTTG+AGG + chr1.4:83424302-83424321 MS.gene73457:CDS 35.0%
! TGAGAAAAATTAGTAGCGGA+TGG + chr1.4:83425052-83425071 MS.gene73457:CDS 35.0%
!! CCTTTAACTTGGACAAGTTT+AGG - chr1.4:83424540-83424559 None:intergenic 35.0%
!! TCAGAATTTTTGAAGGAGCA+TGG - chr1.4:83425697-83425716 None:intergenic 35.0%
AAAGAAATTCACTGCAGGCT+AGG + chr1.4:83425671-83425690 MS.gene73457:CDS 40.0%
AAATGCCTGAATGTGAAGCA+TGG + chr1.4:83424685-83424704 MS.gene73457:CDS 40.0%
AACCATCTTGGATTACCTTG+TGG - chr1.4:83425204-83425223 None:intergenic 40.0%
AAGCACAACATCTTCCTTGT+TGG - chr1.4:83426169-83426188 None:intergenic 40.0%
ATTCGAACCAGCAACAAAGT+TGG + chr1.4:83426074-83426093 MS.gene73457:CDS 40.0%
CTTCACATTCAGGCATTTAG+TGG - chr1.4:83424683-83424702 None:intergenic 40.0%
GACTGCAATGTTACAGTTTG+AGG + chr1.4:83426017-83426036 MS.gene73457:CDS 40.0%
GTACATATTCTTAGGGTTGC+TGG - chr1.4:83423692-83423711 None:intergenic 40.0%
TCATTCTCGAGCAAGTACTT+GGG + chr1.4:83425982-83426001 MS.gene73457:CDS 40.0%
TCTTGGATGATGAGAGAACT+TGG + chr1.4:83425013-83425032 MS.gene73457:CDS 40.0%
TTCATTCTCGAGCAAGTACT+TGG + chr1.4:83425981-83426000 MS.gene73457:CDS 40.0%
TTCGAACCAGCAACAAAGTT+GGG + chr1.4:83426075-83426094 MS.gene73457:CDS 40.0%
!! AACTTTGTTGCTGGTTCGAA+TGG - chr1.4:83426075-83426094 None:intergenic 40.0%
!! TGTTTTTGTGCTTGCCAACA+AGG + chr1.4:83426152-83426171 MS.gene73457:CDS 40.0%
AGACTCCCGATGAGAAAGAA+TGG + chr1.4:83424172-83424191 MS.gene73457:CDS 45.0%
AGTTAGGTCCTGAGGATACA+AGG - chr1.4:83423887-83423906 None:intergenic 45.0%
ATCGTTGTTGTGACTCCCTT+TGG + chr1.4:83425385-83425404 MS.gene73457:intron 45.0%
CAACAAAGTTGGGTGAAGGT+TGG + chr1.4:83426085-83426104 MS.gene73457:CDS 45.0%
CACCACAAGGTAATCCAAGA+TGG + chr1.4:83425199-83425218 MS.gene73457:intron 45.0%
CATCTTGGATTACCTTGTGG+TGG - chr1.4:83425201-83425220 None:intergenic 45.0%
CTCATCATCCAAGACCTGAA+GGG - chr1.4:83425007-83425026 None:intergenic 45.0%
CTTGACCATGCTTCACATTC+AGG - chr1.4:83424693-83424712 None:intergenic 45.0%
GACTCCCGATGAGAAAGAAT+GGG + chr1.4:83424173-83424192 MS.gene73457:CDS 45.0%
GATTCCTTCCTTGTATCCTC+AGG + chr1.4:83423876-83423895 MS.gene73457:intron 45.0%
GGGATGTTTACAGACCAGTA+AGG + chr1.4:83425794-83425813 MS.gene73457:intron 45.0%
GGTCTACTCTTCTTGTGAGA+AGG - chr1.4:83424426-83424445 None:intergenic 45.0%
TCGGGAGTCTTGAGAAACAT+TGG - chr1.4:83424162-83424181 None:intergenic 45.0%
TCGTTGTTGTGACTCCCTTT+GGG + chr1.4:83425386-83425405 MS.gene73457:intron 45.0%
TCTCATCATCCAAGACCTGA+AGG - chr1.4:83425008-83425027 None:intergenic 45.0%
TTCAAGTGAGTTAGGTCCTG+AGG - chr1.4:83423895-83423914 None:intergenic 45.0%
TTGAGTTTCCTTCGTTCCTG+AGG - chr1.4:83425653-83425672 None:intergenic 45.0%
TTGGATTCAAAGGGGTTGGA+AGG + chr1.4:83424227-83424246 MS.gene73457:CDS 45.0%
! TGCTTTTGTTGGCATACCTC+AGG + chr1.4:83425634-83425653 MS.gene73457:intron 45.0%
! TGGATTACCTTGTGGTGGAA+GGG - chr1.4:83425196-83425215 None:intergenic 45.0%
! TTGGATTACCTTGTGGTGGA+AGG - chr1.4:83425197-83425216 None:intergenic 45.0%
AGGTCCTGAGGATACAAGGA+AGG - chr1.4:83423883-83423902 None:intergenic 50.0%
CAACATTCCCTTCCACCACA+AGG + chr1.4:83425186-83425205 MS.gene73457:CDS 50.0%
CCAGCAACAAAGTTGGGTGA+AGG + chr1.4:83426081-83426100 MS.gene73457:CDS 50.0%
CCTTCACCCAACTTTGTTGC+TGG - chr1.4:83426084-83426103 None:intergenic 50.0%
TGAAGCATGGTCAAGAGCAG+AGG + chr1.4:83424698-83424717 MS.gene73457:CDS 50.0%
TTGGCATACCTCAGGAACGA+AGG + chr1.4:83425642-83425661 MS.gene73457:intron 50.0%
CGAAATCGCCCTTCAGGTCT+TGG + chr1.4:83424996-83425015 MS.gene73457:CDS 55.0%
GCAAAGCGAAATCGCCCTTC+AGG + chr1.4:83424990-83425009 MS.gene73457:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 83423685 83426218 83423685 ID=MS.gene73457
chr1.4 mRNA 83423685 83426218 83423685 ID=MS.gene73457.t1;Parent=MS.gene73457
chr1.4 exon 83423685 83423780 83423685 ID=MS.gene73457.t1.exon1;Parent=MS.gene73457.t1
chr1.4 CDS 83423685 83423780 83423685 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83424111 83424558 83424111 ID=MS.gene73457.t1.exon2;Parent=MS.gene73457.t1
chr1.4 CDS 83424111 83424558 83424111 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83424665 83424828 83424665 ID=MS.gene73457.t1.exon3;Parent=MS.gene73457.t1
chr1.4 CDS 83424665 83424828 83424665 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83424949 83425207 83424949 ID=MS.gene73457.t1.exon4;Parent=MS.gene73457.t1
chr1.4 CDS 83424949 83425207 83424949 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83425295 83425372 83425295 ID=MS.gene73457.t1.exon5;Parent=MS.gene73457.t1
chr1.4 CDS 83425295 83425372 83425295 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83425656 83425810 83425656 ID=MS.gene73457.t1.exon6;Parent=MS.gene73457.t1
chr1.4 CDS 83425656 83425810 83425656 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
chr1.4 exon 83425970 83426218 83425970 ID=MS.gene73457.t1.exon7;Parent=MS.gene73457.t1
chr1.4 CDS 83425970 83426218 83425970 ID=cds.MS.gene73457.t1;Parent=MS.gene73457.t1
Gene Sequence

>MS.gene73457

ATGACCAGCAACCCTAAGAATATGTACAGTCTCACTGCTTCTGCTATTCGTTTCTTTAAGATTGTTACCTCAACAAATATTCAAGATGGAACCCTTGTAAGCATTCTTTCCTTCTTTCTTTCCATTTTTTAACTTTTGAACGACCGAAATTTAAGTACTGTATGATAGTTGTTTGTTATGTTTTACGCGGAGATTCCTTCCTTGTATCCTCAGGACCTAACTCACTTGAACTTTTAATTATAAACAAAATATATAAGCCATATGTATCTTTCTAAGAAAAAACAATTTGATTGTTTCTTATAAAAAAAGACTAGAGCTAGAACTTTATACATTTAGATATGACTTCTACTAAGTGCAAGTAAAATGAAAAAAAAAACATAATATCGAGTTTCTGTGCATGCTAACATAAATTTCAATGTACTGCAGAGAATTCCAAATGCTTTCACTGAGAAATATAGTGGTGATTTATCAAAGCCAATGTTTCTCAAGACTCCCGATGAGAAAGAATGGGAAATACATTTGACTAAAAAGGATGGTGATATTTGGATTCAAAGGGGTTGGAAGGAATTTGCTACACATTATTCATTAGATCATGGTCACATGGTTGTGTTTCAATATCAGAAAACCTCTCATTTTGAGGTTTACATATTTGACAAGAGTACTTTTGAAATTGAATATCGTGTTGACGGTAATAATCAACATGAACGGAACAATCCAATTGAAATTTTGGATGAGCAACCTTCTCACAAGAAGAGTAGACCAAAATCACAAATTTCAAGTTCTCAACCACTTAAAAAATTGAGAATTGACACAAGTGAAGATGTCGGAACAAGCTCCAAATCGCAAAACATTCCTAAACTTGTCCAAGTTAAAGGTATAATAAATTCATGATTGGATTAATCTCTATCATTTCATTTATCTAATCAAGATTTTGATTTTTGATGTTGATTTTTCATTACATACCATATTCAATTTCTCAGAAGAGATTGATTCTACCACTAAATGCCTGAATGTGAAGCATGGTCAAGAGCAGAGGAATTCAACTGCTAAAATTCTTGAAGCTTTAAACAAAGCAAAAAACTATGAATCTAACAATCCCTTTTTCACTGCTGTCATGATACATTCATATGTTAATCAATACATGGTGAGTCTCTGAACAGCTTCAATTTTTTATGTTAAGATTGTTTATTATCTTTTTTTTTTAAGCTAATGTATATCTTAAGCAGTTACTACATTTTCTAACTGTCACAAAATGAAAATCCAGTATGTCCCTTTAAATTTTGAACAAAAGTATTTGAAGGAACAGCAAAGCGAAATCGCCCTTCAGGTCTTGGATGATGAGAGAACTTGGATTGTTAAATATTGTATGAGAAAAATTAGTAGCGGATGGAAAACATTTGTATCTGATAATAATTTGAAATTGGGAGATGTTTGCCTTTTTGAGATGATCAATAGAAAATCCTATGCTTTTAAAGTACTAATTTTTCGAGTTGACGAAGAACAACATTCCCTTCCACCACAAGGTAATCCAAGATGGTTTTTTCCTTAAAGTTATTAGTAATCTTTTGGATCTGCACGATCATGATTATATTTTTCATCCTTTGAATCAGTTCATGGAGATGGAATTAACTGGTTAGAAACTGCAGGAATCACAGAAGTTGAAAGTAAAACTATTATGTCGTATAAAGGTAAATCTCAACATCGTTGTTGTGACTCCCTTTGGGAATTTGAAGTTGAATCAAGCAGTATTGTCAAGATGGAATTTGAACAAATCACGATTGTTCTACGATACACTATTTAATACAAAATGCTGTTTAATGGCACTACAATGTAGCTTGAATAATCCTCTATTTTTTTACGACTCACGATTGACAACACTAGAATCAAGAAAAAAAATGCAACTATTGAAGTTTTACAAATAGACATAGCTTATTTGTAAATTGATTATATGCTTTTGTTGGCATACCTCAGGAACGAAGGAAACTCAAAGAAATTCACTGCAGGCTAGGCCATGCTCCTTCAAAAATTCTGAAGCTAAGAAAGAAGCCAATCAATTCACATCCACTTTGGAAAAACCAAATTTCACTATCAATTTGAGATCAAGTCATTGGGATGTTTACAGACCAGTAAGGAATATGTATTTATGATATTTGAACCAAATACATATATCTAGTGATTATGCTTAAAACTGGAGTGGTATTTGTTTCATGTCATGCATTCTAGTAGCAAGTATGCAGTTATTGACATACCCTAACTAACTGTAAAAGAAACACTAATTTGCGCAGCGTGTACGAAATTCATTCTCGAGCAAGTACTTGGGTCCCAAAAAGAAGACTGCAATGTTACAGTTTGAGGAAAAATTGTGGCCTGTACAAGTGATGTATTATCCATTCGAACCAGCAACAAAGTTGGGTGAAGGTTGGAGTCTGTTTGTAGAAGAAAATAAATTGCAGGCTGGAGATGTATGTGTTTTTGTGCTTGCCAACAAGGAAGATGTTGTGCTTGATGTTCATATTTTCAGAGGACGCAGTTAG
Protein sequence

>MS.gene73457.t1

MTSNPKNMYSLTASAIRFFKIVTSTNIQDGTLRIPNAFTEKYSGDLSKPMFLKTPDEKEWEIHLTKKDGDIWIQRGWKEFATHYSLDHGHMVVFQYQKTSHFEVYIFDKSTFEIEYRVDGNNQHERNNPIEILDEQPSHKKSRPKSQISSSQPLKKLRIDTSEDVGTSSKSQNIPKLVQVKEEIDSTTKCLNVKHGQEQRNSTAKILEALNKAKNYESNNPFFTAVMIHSYVNQYMYVPLNFEQKYLKEQQSEIALQVLDDERTWIVKYCMRKISSGWKTFVSDNNLKLGDVCLFEMINRKSYAFKVLIFRVDEEQHSLPPQVHGDGINWLETAGITEVESKTIMSYKGTKETQRNSLQARPCSFKNSEAKKEANQFTSTLEKPNFTINLRSSHWDVYRPRVRNSFSSKYLGPKKKTAMLQFEEKLWPVQVMYYPFEPATKLGEGWSLFVEENKLQAGDVCVFVLANKEDVVLDVHIFRGRS