Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73514.t1 | AES87959.1 | 78.6 | 117 | 11 | 1 | 1 | 103 | 1 | 117 | 5.40E-14 | 87 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73514.t1 | G7JTJ2 | 78.6 | 117 | 11 | 1 | 1 | 103 | 1 | 117 | 3.9e-14 | 87.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene73514.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73514.t1 | MTR_4g039380 | 73.504 | 117 | 17 | 2 | 1 | 103 | 1 | 117 | 8.89e-49 | 151 |
| MS.gene73514.t1 | MTR_4g039640 | 76.923 | 117 | 13 | 2 | 1 | 103 | 1 | 117 | 1.04e-48 | 150 |
| MS.gene73514.t1 | MTR_4g039340 | 65.649 | 131 | 17 | 2 | 1 | 103 | 1 | 131 | 5.03e-46 | 144 |
| MS.gene73514.t1 | MTR_4g039400 | 49.515 | 103 | 31 | 1 | 1 | 103 | 1 | 82 | 9.16e-16 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCATTCATCTGGTGTATA+AGG | 0.184471 | 4.4:+64497543 | None:intergenic |
| TGTGGTTGAGGCTGATTATT+TGG | 0.247122 | 4.4:+64497705 | None:intergenic |
| CTCGATTCTAATATTAAATC+TGG | 0.264896 | 4.4:+64497744 | None:intergenic |
| CTCATTCATCTGGTGTATAA+GGG | 0.356164 | 4.4:+64497544 | None:intergenic |
| AGGTCGAAAGCGCGAACATT+AGG | 0.356569 | 4.4:+64497573 | None:intergenic |
| GGAGGTTGGTTTAAATCAAA+TGG | 0.393156 | 4.4:+64497594 | None:intergenic |
| CTGGTGTATAAGGGATATTA+AGG | 0.496508 | 4.4:+64497553 | None:intergenic |
| TGAGGCTGATTATTTGGTGG+TGG | 0.505165 | 4.4:+64497711 | None:intergenic |
| AAGCGCGAACATTAGGAGGT+TGG | 0.511516 | 4.4:+64497580 | None:intergenic |
| TCGAAAGCGCGAACATTAGG+AGG | 0.542553 | 4.4:+64497576 | None:intergenic |
| ACTGCTAAAGCTTGTGGAGA+TGG | 0.545041 | 4.4:+64497789 | None:intergenic |
| AAATCAAATGGTTGCACGCG+TGG | 0.546490 | 4.4:+64497606 | None:intergenic |
| AATGGTTGCACGCGTGGAGG+TGG | 0.556418 | 4.4:+64497612 | None:intergenic |
| ACATGCACTGCTAAAGCTTG+TGG | 0.565788 | 4.4:+64497783 | None:intergenic |
| GGTTGAGGCTGATTATTTGG+TGG | 0.570406 | 4.4:+64497708 | None:intergenic |
| TCAAATGGTTGCACGCGTGG+AGG | 0.627906 | 4.4:+64497609 | None:intergenic |
| GCATCAACCACAAAATGCAC+CGG | 0.637007 | 4.4:-64497673 | MS.gene73514:CDS |
| ATATTAGAATCGAGTAAACA+AGG | 0.646902 | 4.4:-64497735 | MS.gene73514:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATTAGAATCGAGTAAACA+AGG | - | chr4.4:64497644-64497663 | MS.gene73514:CDS | 25.0% |
| ! | CTCGATTCTAATATTAAATC+TGG | + | chr4.4:64497638-64497657 | None:intergenic | 25.0% |
| CTGGTGTATAAGGGATATTA+AGG | + | chr4.4:64497829-64497848 | None:intergenic | 35.0% | |
| !! | GGAGGTTGGTTTAAATCAAA+TGG | + | chr4.4:64497788-64497807 | None:intergenic | 35.0% |
| TGTGGTTGAGGCTGATTATT+TGG | + | chr4.4:64497677-64497696 | None:intergenic | 40.0% | |
| ! | TGCTCATCATTTTGTGGTTG+AGG | + | chr4.4:64497689-64497708 | None:intergenic | 40.0% |
| !! | GGTTGATGCTCATCATTTTG+TGG | + | chr4.4:64497695-64497714 | None:intergenic | 40.0% |
| !! | GGTTGATGTTCAGCATTTTG+TGG | + | chr4.4:64497737-64497756 | None:intergenic | 40.0% |
| AAATCAAATGGTTGCACGCG+TGG | + | chr4.4:64497776-64497795 | None:intergenic | 45.0% | |
| ACATGCACTGCTAAAGCTTG+TGG | + | chr4.4:64497599-64497618 | None:intergenic | 45.0% | |
| ACTGCTAAAGCTTGTGGAGA+TGG | + | chr4.4:64497593-64497612 | None:intergenic | 45.0% | |
| GCATCAACCACAAAATGCAC+CGG | - | chr4.4:64497706-64497725 | MS.gene73514:CDS | 45.0% | |
| GGTTGAGGCTGATTATTTGG+TGG | + | chr4.4:64497674-64497693 | None:intergenic | 45.0% | |
| ! | TCAGCATTTTGTGGTAGAGC+CGG | + | chr4.4:64497728-64497747 | None:intergenic | 45.0% |
| ! | TGAGGCTGATTATTTGGTGG+TGG | + | chr4.4:64497671-64497690 | None:intergenic | 45.0% |
| AAGCGCGAACATTAGGAGGT+TGG | + | chr4.4:64497802-64497821 | None:intergenic | 50.0% | |
| ! | AGGTCGAAAGCGCGAACATT+AGG | + | chr4.4:64497809-64497828 | None:intergenic | 50.0% |
| ! | TCGAAAGCGCGAACATTAGG+AGG | + | chr4.4:64497806-64497825 | None:intergenic | 50.0% |
| TCAAATGGTTGCACGCGTGG+AGG | + | chr4.4:64497773-64497792 | None:intergenic | 55.0% | |
| ! | CGTGGAGGTGGTGCATTTTG+TGG | + | chr4.4:64497758-64497777 | None:intergenic | 55.0% |
| ! | GGTAGAGCCGGTGCATTTTG+TGG | + | chr4.4:64497716-64497735 | None:intergenic | 55.0% |
| AATGGTTGCACGCGTGGAGG+TGG | + | chr4.4:64497770-64497789 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64497545 | 64497856 | 64497545 | ID=MS.gene73514 |
| chr4.4 | mRNA | 64497545 | 64497856 | 64497545 | ID=MS.gene73514.t1;Parent=MS.gene73514 |
| chr4.4 | exon | 64497545 | 64497856 | 64497545 | ID=MS.gene73514.t1.exon1;Parent=MS.gene73514.t1 |
| chr4.4 | CDS | 64497545 | 64497856 | 64497545 | ID=cds.MS.gene73514.t1;Parent=MS.gene73514.t1 |
| Gene Sequence |
| Protein sequence |
