AlfalfaGEDB | MS.gene73739

M. sativa cultivar XinJiangDaYe / MS.gene73739

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF12874.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73739.t1	XP_024636003.1	93.5	324	21	0	1	324	10	333	1.20E-171	612.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73739.t1	Q943I6	60.5	162	60	2	114	271	268	429	2.8e-57	223.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73739.t1	A0A396IUG6	93.5	324	21	0	1	324	10	333	8.4e-172	612.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene73739.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73739.t1	MTR_2g093960	35.632	174	96	5	104	268	37	203	4.02e-27	110
MS.gene73739.t1	MTR_8g017210	35.897	156	92	3	104	259	67	214	9.54e-27	110
MS.gene73739.t1	MTR_4g059870	35.897	156	93	4	104	259	43	191	3.83e-26	108
MS.gene73739.t1	MTR_2g099990	35.256	156	93	3	104	259	51	198	4.73e-25	105
MS.gene73739.t1	MTR_1g112270	34.228	149	90	3	111	259	56	196	1.20e-24	104
MS.gene73739.t1	MTR_1g112270	34.228	149	90	3	111	259	67	207	1.37e-24	104
MS.gene73739.t1	MTR_1g094115	34.899	149	89	3	111	259	59	199	2.92e-24	103
MS.gene73739.t1	MTR_1g016010	35.099	151	91	3	109	259	51	194	3.04e-23	99.8
MS.gene73739.t1	MTR_2g090745	35.570	149	88	4	111	259	19	159	2.61e-22	97.1
MS.gene73739.t1	MTR_2g099990	36.496	137	79	3	123	259	1	129	2.77e-22	96.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73739.t1	AT1G34370	59.394	165	61	2	113	271	19	183	3.81e-70	220
MS.gene73739.t1	AT1G34370	39.432	317	121	8	26	271	77	393	2.37e-69	224
MS.gene73739.t1	AT1G34370	39.432	317	121	8	26	271	77	393	2.37e-69	224
MS.gene73739.t1	AT5G22890	58.125	160	61	1	113	266	202	361	2.84e-66	213
MS.gene73739.t1	AT5G66730	37.821	156	89	3	104	259	37	184	1.47e-27	112
MS.gene73739.t1	AT5G44160	35.897	156	92	4	104	259	42	189	4.41e-27	110
MS.gene73739.t1	AT5G44160	36.424	151	88	4	109	259	8	150	7.45e-27	109
MS.gene73739.t1	AT1G03840	37.086	151	87	4	109	259	51	193	1.29e-26	110
MS.gene73739.t1	AT3G45260	35.762	151	90	3	109	259	49	192	4.39e-26	107
MS.gene73739.t1	AT3G45260	35.762	151	90	3	109	259	49	192	4.39e-26	107

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Find 97 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATTTGGTTGGAGACGG+CGG	0.627067	3.1:+70110108	None:intergenic
AAAGGCTTCGCTAAAGCTTC+CGG	0.425013	3.1:+70109830	None:intergenic
AAAGGTGATGGAGGAGAAGA+CGG	0.568206	3.1:+70110033	None:intergenic
AACGCCGCCGCCCTCGTTCC+CGG	0.371943	3.1:-70110061	MS.gene73739:CDS
AACGGACACGAGAACCGTGT+TGG	0.584128	3.1:+70109791	None:intergenic
AAGAGTCATATGAGGCAATG+TGG	0.740474	3.1:-70109617	MS.gene73739:CDS
AAGCGGAACTTGAGGGTCGG+GGG	0.565688	3.1:+70110228	None:intergenic
AAGTATCTGGGTAGTGTGAT+GGG	0.491876	3.1:+70110276	None:intergenic
AATCTTCCACGGCGGTGAGG+GGG	0.520341	3.1:+70109993	None:intergenic
AATTGTGGAGCTCGACGCCA+TGG	0.568707	3.1:-70109960	MS.gene73739:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATTACACGAATACATCTT+CGG	+	chr3.1:70109970-70109989	None:intergenic	30.0%
GAAGAATTATTCGATGCTTT+CGG	-	chr3.1:70109999-70110018	MS.gene73739:CDS	30.0%
TCGAGTAAAGATGATGAAAT+TGG	-	chr3.1:70110216-70110235	MS.gene73739:CDS	30.0%
TTTCTGTTACATCCTTCAAA+CGG	+	chr3.1:70109874-70109893	None:intergenic	30.0%
GAATGAAGATCCTATGGAAA+TGG	-	chr3.1:70110191-70110210	MS.gene73739:CDS	35.0%
GTTAATTGATCATTGCTGAC+AGG	+	chr3.1:70109517-70109536	None:intergenic	35.0%
TCTAGGAAGGATAAGTTGTT+TGG	-	chr3.1:70110087-70110106	MS.gene73739:CDS	35.0%
AAGAGTCATATGAGGCAATG+TGG	-	chr3.1:70110027-70110046	MS.gene73739:CDS	40.0%
AAGTATCTGGGTAGTGTGAT+GGG	+	chr3.1:70109371-70109390	None:intergenic	40.0%
AGATTCCGATTGCGAAATTG+TGG	-	chr3.1:70109669-70109688	MS.gene73739:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	70109346	70110320	70109346	ID=MS.gene73739
chr3.1	mRNA	70109346	70110320	70109346	ID=MS.gene73739.t1;Parent=MS.gene73739
chr3.1	exon	70109346	70110320	70109346	ID=MS.gene73739.t1.exon1;Parent=MS.gene73739.t1
chr3.1	CDS	70109346	70110320	70109346	ID=cds.MS.gene73739.t1;Parent=MS.gene73739.t1

Gene Sequence

>MS.gene73739

ATGTCTAATCCGAACCCAGATTCCCATCACACTACCCAGATACTTCATCCACTGCAAAATCCAACTAATACCCCCGACCCTCAAGTTCCGCTTCGGAATCTATCCTTGGTTCAGACAAGCATCGATTCTCTCCGAACCTTCCTGTCCCAATCCATCACCACCAACGCTCCTGTCAGCAATGATCAATTAACCGCCGTCTCCAACCAAATCTTTGCCGTCCTCCACCATACCATAGTCAACGCCGCCGCCCTCGTTCCCGGTGCCTCCGTCTTCTCCTCCATCACCTTTGATTCAGTCAACAACAACCCCCTCACCGCCGTGGAAGATTCCGATTGCGAAATTGTGGAGCTCGACGCCATGGAATTGCTAGCTGAACATCTCCATTTCTGCGAGATATGCGGCAAAGGCTTCAAGCGCGACGCGAATCTCCGGATGCACATGAGAGCACACGGGAATCAGTTCAAGACGCCGGAAGCTTTAGCGAAGCCTTTAACCGTGCGTCGTCGTCCAACACGGTTCTCGTGTCCGTTTGAAGGATGTAACAGAAACAAGAAGCATAAGAAATTCAAAGCATTGAAATCGGTGATTTGTGTGAAGACTCATTTCAAGAGGAGTCATTGTCCGAAGATGTATTCGTGTAATCTTTGTCGTAAGAAGAATTATTCGATGCTTTCGGATTTGAAGAGTCATATGAGGCAATGTGGTGAGTCTAAGTGGAAGTGTTCTTGTGGGAGTACTTTTTCTAGGAAGGATAAGTTGTTTGGACATGTTGCTTTGTTTGAGGGTCATATGCCTGCTGTTATGTTGGAAGATGAGGAGAAAGGGAAGCAAGTTGTTGCTGAGGAGAATGAAGATCCTATGGAAATGGCATCGAGTAAAGATGATGAAATTGGTATGGATGAAGAGTTCTTTCATGATTTTGGGTCTATTGATAACTATTGCTTTGATGAAATTTTGGGATTTCGTCGTAGCTAG

Protein sequence

>MS.gene73739.t1

MSNPNPDSHHTTQILHPLQNPTNTPDPQVPLRNLSLVQTSIDSLRTFLSQSITTNAPVSNDQLTAVSNQIFAVLHHTIVNAAALVPGASVFSSITFDSVNNNPLTAVEDSDCEIVELDAMELLAEHLHFCEICGKGFKRDANLRMHMRAHGNQFKTPEALAKPLTVRRRPTRFSCPFEGCNRNKKHKKFKALKSVICVKTHFKRSHCPKMYSCNLCRKKNYSMLSDLKSHMRQCGESKWKCSCGSTFSRKDKLFGHVALFEGHMPAVMLEDEEKGKQVVAEENEDPMEMASSKDDEIGMDEEFFHDFGSIDNYCFDEILGFRRS