Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73903.t1 | XP_024639353.1 | 84.4 | 128 | 20 | 0 | 51 | 178 | 4 | 131 | 2.10E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73903.t1 | G7JX45 | 54.5 | 178 | 12 | 1 | 1 | 178 | 1 | 109 | 2.6e-42 | 181.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene73903.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73903.t1 | MTR_5g053510 | 89.412 | 85 | 9 | 0 | 94 | 178 | 25 | 109 | 1.67e-51 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGAATTCTTCAAGAAATTT+TGG | 0.074446 | 8.4:+9470332 | None:intergenic |
| TCGATGATTTGAAACCAATT+TGG | 0.219001 | 8.4:-9470313 | MS.gene73903:CDS |
| CAGACTTCAATGATTTCTTA+TGG | 0.226332 | 8.4:-9469886 | MS.gene73903:CDS |
| GATTTGCATTGCACAGAAAA+AGG | 0.262758 | 8.4:+9469846 | None:intergenic |
| TGGAATCTGATAGATGTTAA+TGG | 0.281895 | 8.4:-9470293 | MS.gene73903:CDS |
| TGTACTCAAACGACAAGTTT+TGG | 0.321936 | 8.4:-9470532 | MS.gene73903:CDS |
| ACATCTATCAGATTCCAAAT+TGG | 0.325811 | 8.4:+9470299 | None:intergenic |
| AACGAATAGTGTCTCCAATC+TGG | 0.387174 | 8.4:+9469720 | None:intergenic |
| AGACTTCAATGATTTCTTAT+GGG | 0.387413 | 8.4:-9469885 | MS.gene73903:CDS |
| AATATATACAAAACCTGATC+TGG | 0.390568 | 8.4:+9470486 | None:intergenic |
| AAATAAAATGATTTGCGGTT+CGG | 0.410181 | 8.4:+9470063 | None:intergenic |
| AACTGTACATCTTCCAGATC+AGG | 0.417936 | 8.4:-9470499 | MS.gene73903:intron |
| TATTCAGCGATTCATTGCTT+TGG | 0.421950 | 8.4:+9469789 | None:intergenic |
| GATGGCAGAAAGATCTGAAT+TGG | 0.422050 | 8.4:-9470016 | MS.gene73903:intron |
| GCTGTAACAAACTCTTCATT+CGG | 0.423810 | 8.4:+9469824 | None:intergenic |
| CTCTTTCCACTTCAAATGGC+TGG | 0.424705 | 8.4:-9470564 | None:intergenic |
| CATAAGAAATCATTGAAGTC+TGG | 0.456714 | 8.4:+9469887 | None:intergenic |
| TGCTTCTGCTGTGGAAGGGA+GGG | 0.473215 | 8.4:+9470094 | None:intergenic |
| TGTCAAAATAAAATGATTTG+CGG | 0.475450 | 8.4:+9470058 | None:intergenic |
| TTCCTATAGACATGAACAAA+AGG | 0.480398 | 8.4:+9469671 | None:intergenic |
| ATTCGTTTCAAGTTCTTCGA+TGG | 0.500169 | 8.4:-9469704 | MS.gene73903:CDS |
| TTCGTTTCAAGTTCTTCGAT+GGG | 0.505866 | 8.4:-9469703 | MS.gene73903:CDS |
| AACAACAATGCATTCCAGAT+TGG | 0.510902 | 8.4:-9469734 | MS.gene73903:CDS |
| CACTTTGACTCATTTGCAGA+TGG | 0.525652 | 8.4:-9470034 | MS.gene73903:CDS |
| GTGCTTCTGCTGTGGAAGGG+AGG | 0.535843 | 8.4:+9470093 | None:intergenic |
| TTTGTGCTTCTGCTGTGGAA+GGG | 0.552787 | 8.4:+9470090 | None:intergenic |
| AAGAAATCATTGAAGTCTGG+TGG | 0.620565 | 8.4:+9469890 | None:intergenic |
| GAAGGGAGGGAATTCATCAG+TGG | 0.689324 | 8.4:+9470107 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTATTAAGTTTACAAAC+AGG | + | chr8.4:9469793-9469812 | None:intergenic | 15.0% |
| !! | TATTATTAAGTTTACAAACA+GGG | + | chr8.4:9469792-9469811 | None:intergenic | 15.0% |
| !!! | AAAATTGGTTTTTGTTAAAT+TGG | + | chr8.4:9470283-9470302 | None:intergenic | 15.0% |
| !! | ATACTAAGTTAAAAAATGGT+TGG | - | chr8.4:9470441-9470460 | MS.gene73903:intron | 20.0% |
| !! | CTAATAACATATATTTGATC+CGG | - | chr8.4:9470229-9470248 | MS.gene73903:intron | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+TGG | - | chr8.4:9470437-9470456 | MS.gene73903:intron | 20.0% |
| !! | TACTAAGTTAAAAAATGGTT+GGG | - | chr8.4:9470442-9470461 | MS.gene73903:intron | 20.0% |
| !! | TGTCAAAATAAAATGATTTG+CGG | + | chr8.4:9470155-9470174 | None:intergenic | 20.0% |
| ! | AATATATACAAAACCTGATC+TGG | + | chr8.4:9469727-9469746 | None:intergenic | 25.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | - | chr8.4:9469950-9469969 | MS.gene73903:intron | 25.0% |
| ! | ACATAATTTGGTTAGAAAAC+CGG | + | chr8.4:9470251-9470270 | None:intergenic | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | - | chr8.4:9470325-9470344 | MS.gene73903:CDS | 25.0% |
| !! | AAACCAATTTTGCAAATTAC+AGG | - | chr8.4:9470292-9470311 | MS.gene73903:CDS | 25.0% |
| !! | AAATAAAATGATTTGCGGTT+CGG | + | chr8.4:9470150-9470169 | None:intergenic | 25.0% |
| !! | CGAATTCTTCAAGAAATTTT+GGG | + | chr8.4:9469880-9469899 | None:intergenic | 25.0% |
| !! | TAAATGTTGATTATGTGTAC+AGG | - | chr8.4:9469838-9469857 | MS.gene73903:CDS | 25.0% |
| !! | TCGAATTCTTCAAGAAATTT+TGG | + | chr8.4:9469881-9469900 | None:intergenic | 25.0% |
| !!! | AAGAAATTTTGGGTTAACTT+CGG | + | chr8.4:9469870-9469889 | None:intergenic | 25.0% |
| AAATCGTGCTAAACTATGTT+GGG | - | chr8.4:9470041-9470060 | MS.gene73903:CDS | 30.0% | |
| ACATCTATCAGATTCCAAAT+TGG | + | chr8.4:9469914-9469933 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | - | chr8.4:9470324-9470343 | MS.gene73903:CDS | 30.0% | |
| CATAAGAAATCATTGAAGTC+TGG | + | chr8.4:9470326-9470345 | None:intergenic | 30.0% | |
| CTTCAAGAGTTAGTATGAAT+CGG | + | chr8.4:9470072-9470091 | None:intergenic | 30.0% | |
| TGGAATCTGATAGATGTTAA+TGG | - | chr8.4:9469917-9469936 | MS.gene73903:intron | 30.0% | |
| TTCCTATAGACATGAACAAA+AGG | + | chr8.4:9470542-9470561 | None:intergenic | 30.0% | |
| ! | AGAGATTTTTACCATTTCGA+AGG | - | chr8.4:9470010-9470029 | MS.gene73903:intron | 30.0% |
| ! | GAACCTGTAATTTGCAAAAT+TGG | + | chr8.4:9470298-9470317 | None:intergenic | 30.0% |
| ! | TCGATGATTTGAAACCAATT+TGG | - | chr8.4:9469897-9469916 | MS.gene73903:CDS | 30.0% |
| ! | TGGTGAAAAAGCACATAATT+TGG | + | chr8.4:9470263-9470282 | None:intergenic | 30.0% |
| AACAACAATGCATTCCAGAT+TGG | - | chr8.4:9470476-9470495 | MS.gene73903:intron | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | + | chr8.4:9470323-9470342 | None:intergenic | 35.0% | |
| CAAATCGTGCTAAACTATGT+TGG | - | chr8.4:9470040-9470059 | MS.gene73903:CDS | 35.0% | |
| CGATTCATACTAACTCTTGA+AGG | - | chr8.4:9470070-9470089 | MS.gene73903:CDS | 35.0% | |
| GATTTGCATTGCACAGAAAA+AGG | + | chr8.4:9470367-9470386 | None:intergenic | 35.0% | |
| GCTGTAACAAACTCTTCATT+CGG | + | chr8.4:9470389-9470408 | None:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | + | chr8.4:9470424-9470443 | None:intergenic | 35.0% | |
| ! | GTACTCAAACGACAAGTTTT+GGG | - | chr8.4:9469679-9469698 | MS.gene73903:CDS | 35.0% |
| ! | GTCCTTTTGTTCATGTCTAT+AGG | - | chr8.4:9470537-9470556 | MS.gene73903:CDS | 35.0% |
| ! | TGTACTCAAACGACAAGTTT+TGG | - | chr8.4:9469678-9469697 | MS.gene73903:CDS | 35.0% |
| !! | ATTCGTTTCAAGTTCTTCGA+TGG | - | chr8.4:9470506-9470525 | MS.gene73903:CDS | 35.0% |
| !! | TTCGTTTCAAGTTCTTCGAT+GGG | - | chr8.4:9470507-9470526 | MS.gene73903:CDS | 35.0% |
| AACGAATAGTGTCTCCAATC+TGG | + | chr8.4:9470493-9470512 | None:intergenic | 40.0% | |
| AACTGTACATCTTCCAGATC+AGG | - | chr8.4:9469711-9469730 | MS.gene73903:CDS | 40.0% | |
| CACTTTGACTCATTTGCAGA+TGG | - | chr8.4:9470176-9470195 | MS.gene73903:intron | 40.0% | |
| GATGGCAGAAAGATCTGAAT+TGG | - | chr8.4:9470194-9470213 | MS.gene73903:intron | 40.0% | |
| TCCATCCTGTAGTGAGTAAA+GGG | + | chr8.4:9469982-9470001 | None:intergenic | 40.0% | |
| TTTGTCTGATGCCTTCGAAA+TGG | + | chr8.4:9470024-9470043 | None:intergenic | 40.0% | |
| ATCCTCCCTTTACTCACTAC+AGG | - | chr8.4:9469974-9469993 | MS.gene73903:intron | 45.0% | |
| ATCCTGTAGTGAGTAAAGGG+AGG | + | chr8.4:9469979-9469998 | None:intergenic | 45.0% | |
| CTCCATCCTGTAGTGAGTAA+AGG | + | chr8.4:9469983-9470002 | None:intergenic | 45.0% | |
| TCCCTTTACTCACTACAGGA+TGG | - | chr8.4:9469978-9469997 | MS.gene73903:intron | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | + | chr8.4:9470123-9470142 | None:intergenic | 45.0% | |
| ! | TTTTGTGCTTCTGCTGTGGA+AGG | + | chr8.4:9470124-9470143 | None:intergenic | 45.0% |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | - | chr8.4:9469682-9469701 | MS.gene73903:CDS | 45.0% |
| !!! | GTAGTTTTGTGCTTCTGCTG+TGG | + | chr8.4:9470128-9470147 | None:intergenic | 45.0% |
| GAAGGGAGGGAATTCATCAG+TGG | + | chr8.4:9470106-9470125 | None:intergenic | 50.0% | |
| TGCTTCTGCTGTGGAAGGGA+GGG | + | chr8.4:9470119-9470138 | None:intergenic | 55.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | + | chr8.4:9470120-9470139 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9469661 | 9470571 | 9469661 | ID=MS.gene73903 |
| chr8.4 | mRNA | 9469661 | 9470571 | 9469661 | ID=MS.gene73903.t1;Parent=MS.gene73903 |
| chr8.4 | exon | 9470500 | 9470571 | 9470500 | ID=MS.gene73903.t1.exon1;Parent=MS.gene73903.t1 |
| chr8.4 | CDS | 9470500 | 9470571 | 9470500 | ID=cds.MS.gene73903.t1;Parent=MS.gene73903.t1 |
| chr8.4 | exon | 9470280 | 9470372 | 9470280 | ID=MS.gene73903.t1.exon2;Parent=MS.gene73903.t1 |
| chr8.4 | CDS | 9470280 | 9470372 | 9470280 | ID=cds.MS.gene73903.t1;Parent=MS.gene73903.t1 |
| chr8.4 | exon | 9470017 | 9470130 | 9470017 | ID=MS.gene73903.t1.exon3;Parent=MS.gene73903.t1 |
| chr8.4 | CDS | 9470017 | 9470130 | 9470017 | ID=cds.MS.gene73903.t1;Parent=MS.gene73903.t1 |
| chr8.4 | exon | 9469661 | 9469918 | 9469661 | ID=MS.gene73903.t1.exon4;Parent=MS.gene73903.t1 |
| chr8.4 | CDS | 9469661 | 9469918 | 9469661 | ID=cds.MS.gene73903.t1;Parent=MS.gene73903.t1 |
| Gene Sequence |
| Protein sequence |
