AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73917

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73917.t1 MTR_5g058860 96.654 508 11 3 5 508 1 506 0.0 1007
MS.gene73917.t1 MTR_3g072710 61.014 513 161 12 16 502 4 503 0.0 572
MS.gene73917.t1 MTR_8g093070 37.313 402 214 14 107 499 124 496 7.19e-63 213
MS.gene73917.t1 MTR_7g027190 35.749 414 218 14 108 500 288 674 7.93e-63 217
MS.gene73917.t1 MTR_7g074650 35.027 374 218 10 126 498 442 791 1.13e-52 191
MS.gene73917.t1 MTR_3g065980 34.314 408 209 15 116 498 166 539 2.10e-51 184
MS.gene73917.t1 MTR_1g069725 33.168 404 235 11 100 500 93 464 3.71e-48 173
MS.gene73917.t1 MTR_4g076020 35.262 363 192 12 151 506 110 436 3.92e-47 169
MS.gene73917.t1 MTR_8g442410 28.161 522 317 16 6 498 32 524 9.06e-44 162
MS.gene73917.t1 MTR_5g097480 30.591 389 242 11 119 498 174 543 3.41e-43 161
MS.gene73917.t1 MTR_1g096030 30.918 414 240 12 112 501 70 461 1.67e-41 155
MS.gene73917.t1 MTR_3g056110 32.055 365 222 11 143 498 194 541 2.25e-41 156
MS.gene73917.t1 MTR_1g029420 31.525 387 237 14 121 498 224 591 1.01e-40 155
MS.gene73917.t1 MTR_2g082090 30.928 388 242 11 119 498 209 578 2.45e-40 154
MS.gene73917.t1 MTR_4g104020 29.440 411 258 10 103 500 130 521 6.47e-40 151
MS.gene73917.t1 MTR_4g077760 31.897 348 221 7 153 498 221 554 1.96e-39 150
MS.gene73917.t1 MTR_3g089055 30.052 386 244 11 121 498 197 564 3.46e-39 150
MS.gene73917.t1 MTR_3g089055 30.052 386 244 11 121 498 202 569 3.66e-39 150
MS.gene73917.t1 MTR_4g102790 30.941 404 234 17 115 499 285 662 6.13e-38 147
MS.gene73917.t1 MTR_4g133660 29.692 357 226 10 153 500 204 544 2.33e-37 144
MS.gene73917.t1 MTR_5g094450 27.826 460 293 13 54 498 96 531 1.03e-34 137
MS.gene73917.t1 MTR_2g097410 27.189 434 268 15 89 499 333 741 2.32e-33 134
MS.gene73917.t1 MTR_2g026250 26.611 357 237 10 153 500 257 597 3.86e-33 133
MS.gene73917.t1 MTR_4g064200 28.133 391 244 15 122 499 284 650 3.08e-30 125
MS.gene73917.t1 MTR_2g097467 27.095 358 232 9 152 499 316 654 1.04e-29 123
MS.gene73917.t1 MTR_4g064150 28.967 397 238 15 122 499 362 733 2.54e-29 122
MS.gene73917.t1 MTR_0092s0100 30.052 386 241 14 119 500 372 732 2.88e-28 119
MS.gene73917.t1 MTR_0092s0100 30.052 386 241 14 119 500 369 729 3.03e-28 119
MS.gene73917.t1 MTR_2g034250 25.926 405 264 11 111 498 199 584 6.76e-28 117
MS.gene73917.t1 MTR_4g064120 25.056 443 282 14 80 501 212 625 1.14e-27 117
MS.gene73917.t1 MTR_7g062120 25.510 392 261 9 117 498 369 739 1.29e-27 117
MS.gene73917.t1 MTR_4g064160 26.047 430 280 13 89 499 274 684 3.36e-27 116
MS.gene73917.t1 MTR_8g077940 29.067 375 234 13 134 500 192 542 4.10e-27 115
MS.gene73917.t1 MTR_3g022830 25.373 402 229 11 151 498 51 435 6.76e-27 113
MS.gene73917.t1 MTR_2g034260 26.442 416 263 15 101 498 193 583 7.33e-27 114
MS.gene73917.t1 MTR_2g097463 26.816 358 233 10 152 499 317 655 8.78e-27 114
MS.gene73917.t1 MTR_2g097473 26.536 358 234 9 152 499 316 654 8.84e-27 114
MS.gene73917.t1 MTR_4g064180 25.995 427 260 17 99 501 231 625 3.95e-26 112
MS.gene73917.t1 MTR_2g099110 29.121 364 216 13 70 411 8 351 5.74e-26 110
MS.gene73917.t1 MTR_2g034280 23.899 477 319 15 38 498 126 574 2.87e-25 109
MS.gene73917.t1 MTR_2g097390 26.615 387 253 13 122 498 275 640 7.94e-25 108
MS.gene73917.t1 MTR_4g097080 26.042 384 265 10 121 498 132 502 1.04e-24 107
MS.gene73917.t1 MTR_4g076140 32.479 234 134 6 108 337 34 247 2.06e-24 106
MS.gene73917.t1 MTR_5g015490 27.681 401 243 15 121 498 113 489 8.22e-24 104
MS.gene73917.t1 MTR_2g097350 26.343 391 249 15 122 498 274 639 1.53e-23 104
MS.gene73917.t1 MTR_2g097310 24.163 418 277 12 102 499 241 638 2.55e-23 103
MS.gene73917.t1 MTR_5g009080 31.780 236 137 7 109 340 43 258 4.11e-23 102
MS.gene73917.t1 MTR_7g109580 24.691 405 265 15 111 501 178 556 2.72e-22 100
MS.gene73917.t1 MTR_2g097380 24.650 357 214 10 152 499 251 561 8.94e-22 99.0
MS.gene73917.t1 MTR_4g026485 25.455 385 251 14 123 500 270 625 2.66e-19 91.7
MS.gene73917.t1 MTR_7g069740 26.730 419 256 18 99 498 197 583 1.54e-18 89.4
MS.gene73917.t1 MTR_7g057230 27.742 310 195 10 200 498 365 656 3.14e-15 79.0
MS.gene73917.t1 MTR_1g086970 22.983 409 274 13 111 501 95 480 5.81e-15 77.8
MS.gene73917.t1 MTR_2g089100 24.634 410 271 12 116 499 46 443 1.51e-14 76.3
MS.gene73917.t1 MTR_8g020840 23.929 397 264 14 121 498 170 547 1.79e-12 70.1
MS.gene73917.t1 MTR_6g047750 26.087 391 249 15 123 498 296 661 1.74e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73917.t1 AT4G08250 51.324 491 203 14 23 498 7 476 7.58e-149 435
MS.gene73917.t1 AT3G54220 36.139 404 224 13 106 502 277 653 7.32e-55 195
MS.gene73917.t1 AT3G03450 31.034 493 282 16 41 498 75 544 2.33e-52 186
MS.gene73917.t1 AT5G41920 35.821 402 220 14 106 498 27 399 2.48e-52 183
MS.gene73917.t1 AT5G17490 35.606 396 220 15 116 504 154 521 3.87e-50 180
MS.gene73917.t1 AT1G66350 32.737 391 221 13 116 498 149 505 4.67e-46 168
MS.gene73917.t1 AT2G01570 32.997 397 228 16 111 498 213 580 2.06e-44 165
MS.gene73917.t1 AT1G14920 31.959 388 234 13 116 498 166 528 3.30e-43 161
MS.gene73917.t1 AT5G48150 29.241 448 287 12 59 498 64 489 2.13e-42 158
MS.gene73917.t1 AT5G48150 29.241 448 287 12 59 498 64 489 2.13e-42 158
MS.gene73917.t1 AT5G48150 29.241 448 287 12 59 498 64 489 2.13e-42 158
MS.gene73917.t1 AT5G48150 29.241 448 287 12 59 498 64 489 2.13e-42 158
MS.gene73917.t1 AT2G04890 30.612 392 247 11 110 498 43 412 1.50e-40 151
MS.gene73917.t1 AT1G55580 32.208 385 206 12 155 498 74 444 5.36e-40 150
MS.gene73917.t1 AT4G17230 31.990 397 230 12 117 498 153 524 1.17e-39 151
MS.gene73917.t1 AT4G17230 31.990 397 230 12 117 498 153 524 1.17e-39 151
MS.gene73917.t1 AT1G50600 30.663 362 231 8 143 498 178 525 3.07e-39 150
MS.gene73917.t1 AT1G50600 30.939 362 230 8 143 498 253 600 6.18e-39 150
MS.gene73917.t1 AT5G66770 30.693 404 261 10 99 498 195 583 6.33e-39 150
MS.gene73917.t1 AT1G50600 30.939 362 230 8 143 498 249 596 6.68e-39 150
MS.gene73917.t1 AT3G50650 32.787 366 216 10 144 498 195 541 1.58e-38 148
MS.gene73917.t1 AT1G63100 29.512 410 245 13 112 499 242 629 2.60e-34 137
MS.gene73917.t1 AT1G63100 29.512 410 245 13 112 499 267 654 3.97e-34 136
MS.gene73917.t1 AT1G21450 28.369 423 266 15 87 498 196 592 5.62e-32 130
MS.gene73917.t1 AT4G37650 28.030 396 253 11 122 498 146 528 6.18e-30 123
MS.gene73917.t1 AT4G36710 29.380 371 227 13 138 498 139 484 2.52e-29 121
MS.gene73917.t1 AT3G60630 28.606 409 260 11 107 500 232 623 3.10e-29 122
MS.gene73917.t1 AT2G29065 27.569 399 246 11 122 499 252 628 3.77e-29 121
MS.gene73917.t1 AT3G46600 25.000 444 265 14 84 498 174 578 6.63e-28 117
MS.gene73917.t1 AT1G07530 26.512 430 288 12 78 499 356 765 7.18e-28 118
MS.gene73917.t1 AT3G46600 27.027 407 229 14 121 498 3 370 1.00e-27 114
MS.gene73917.t1 AT3G46600 25.000 444 265 14 84 498 142 546 1.33e-27 116
MS.gene73917.t1 AT2G29060 26.566 399 255 14 119 501 281 657 3.87e-27 115
MS.gene73917.t1 AT2G29060 26.566 399 255 14 119 501 318 694 5.16e-27 115
MS.gene73917.t1 AT5G59450 25.740 439 279 13 83 498 183 597 1.55e-26 114
MS.gene73917.t1 AT1G07520 25.231 432 287 9 81 499 285 693 2.43e-25 110
MS.gene73917.t1 AT1G07520 25.231 432 287 9 81 499 286 694 2.44e-25 110
MS.gene73917.t1 AT1G07520 25.000 448 288 10 71 499 111 529 3.14e-25 109
MS.gene73917.t1 AT1G07520 25.000 448 288 10 71 499 111 529 3.14e-25 109
MS.gene73917.t1 AT2G45160 27.295 403 242 16 119 500 268 640 2.96e-24 107
MS.gene73917.t1 AT2G37650 25.964 389 259 11 119 498 332 700 2.91e-23 104
MS.gene73917.t1 AT2G37650 25.840 387 262 10 119 498 344 712 3.32e-23 103
MS.gene73917.t1 AT1G50420 31.579 228 136 6 113 337 48 258 3.92e-22 99.8
MS.gene73917.t1 AT1G50420 30.921 152 102 3 350 500 331 480 2.64e-12 69.3
MS.gene73917.t1 AT4G00150 27.865 384 238 13 123 502 209 557 6.96e-22 99.4
MS.gene73917.t1 AT5G52510 28.135 327 194 12 196 498 330 639 1.46e-16 83.2
MS.gene73917.t1 AT3G49950 25.000 400 263 14 121 501 29 410 5.63e-14 74.3
MS.gene73917.t1 AT3G13840 24.167 480 302 14 58 498 53 509 3.68e-13 72.0

Find 168 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGTTTCCTATGAATAAA+TGG 0.181822 5.4:+46167007 MS.gene73917:CDS
GACTCTTGTTTGTGCCTGTT+AGG 0.230972 5.4:-46166201 None:intergenic
TTGTCAAGCAAAGTTGTTAT+TGG 0.254379 5.4:+46167191 MS.gene73917:CDS
CGGTGCCGTTTAGCAGAGTT+TGG 0.274447 5.4:-46166324 None:intergenic
CTTCACCAGTCTTGTACAAC+TGG 0.307827 5.4:-46167086 None:intergenic
TTGGAGAATTCTAGAAAATC+TGG 0.308659 5.4:-46165878 None:intergenic
ACGACCTTGTTATCTAGATT+TGG 0.309984 5.4:-46166046 None:intergenic
TTTGCCACTGTTCAAGAAAC+TGG 0.314348 5.4:+46166658 MS.gene73917:CDS
GGAAACAATAAGTTGGTGTT+AGG 0.333033 5.4:+46167249 MS.gene73917:CDS
GGGGAGTGGTTGGGTGTGGA+AGG 0.333319 5.4:+46167135 MS.gene73917:CDS
CCGTCTAGTGCACCTCCTTA+TGG 0.339880 5.4:+46166165 MS.gene73917:CDS
TGTCAAGCAAAGTTGTTATT+GGG 0.347692 5.4:+46167192 MS.gene73917:CDS
GGCTGCTTTCTGCTTCTGTT+TGG 0.348023 5.4:+46167286 MS.gene73917:CDS
TTGAACGGTGCCAAGGAGTT+AGG 0.348949 5.4:+46166868 MS.gene73917:CDS
ATTTGCCACATCTTAGTTAC+CGG 0.358640 5.4:+46166821 MS.gene73917:CDS
GCTTGTTCGCGGAGAGGCTT+TGG 0.359996 5.4:+46166780 MS.gene73917:CDS
AGGCTTTGTGGGTTTGTTCA+TGG 0.365262 5.4:+46166942 MS.gene73917:CDS
CTCCTAGCCCATTTATTCAT+AGG 0.371100 5.4:-46167015 None:intergenic
TTGGTCCCCTCTCCTTGACT+TGG 0.383703 5.4:+46165946 MS.gene73917:CDS
GCGGCAGCGGAGGCCCTAAC+AGG 0.386266 5.4:+46166187 MS.gene73917:CDS
CATAGTGGACTTTGATATCA+TGG 0.386901 5.4:+46166507 MS.gene73917:CDS
ACAATAAGTTGGTGTTAGGT+TGG 0.396110 5.4:+46167253 MS.gene73917:CDS
CATCAACGATGCAGGCTTTG+TGG 0.396922 5.4:+46166930 MS.gene73917:CDS
GGAGCAAGAGAGGTTCTCTT+GGG 0.397324 5.4:+46167113 MS.gene73917:CDS
TTTCATGAGTAACAGCTTCA+AGG 0.400010 5.4:-46166474 None:intergenic
ATGTATGACTCGTTCGAGGC+TGG 0.402016 5.4:+46166988 MS.gene73917:CDS
AGGAGCAAGAGAGGTTCTCT+TGG 0.407648 5.4:+46167112 MS.gene73917:CDS
CGTCAAGTTTGCTCATTTCA+CGG 0.410498 5.4:+46166438 MS.gene73917:CDS
ATCAACGATGCAGGCTTTGT+GGG 0.413507 5.4:+46166931 MS.gene73917:CDS
ACTCTTGTTTGTGCCTGTTA+GGG 0.415722 5.4:-46166200 None:intergenic
GGGAGTGGTTGGGTGTGGAA+GGG 0.418452 5.4:+46167136 MS.gene73917:CDS
CACAAAGCCTGCATCGTTGA+TGG 0.420533 5.4:-46166929 None:intergenic
TGTATGACTCGTTCGAGGCT+GGG 0.428573 5.4:+46166989 MS.gene73917:CDS
GGGCCTCCGCTGCCGCCATA+AGG 0.429881 5.4:-46166180 None:intergenic
TGCGAAGATGTGGGCCCGGC+AGG 0.434472 5.4:-46166582 None:intergenic
CTCCGTTGTCGAATTTGAGT+TGG 0.441756 5.4:-46166110 None:intergenic
CTTCTGATGACGACACCATT+TGG 0.442452 5.4:+46165927 MS.gene73917:CDS
TTATCTAGATTTGGAGCAAC+GGG 0.447526 5.4:-46166037 None:intergenic
CCTCCTTATGGCGGCAGCGG+AGG 0.448204 5.4:+46166177 MS.gene73917:CDS
GCCACTGAGCCCATTATTCG+TGG 0.457871 5.4:-46167063 None:intergenic
TGGGTATAGAGTGGAAGAGT+TGG 0.459148 5.4:+46167227 MS.gene73917:CDS
TCGCGAAAAGAAGGCCTGCC+GGG 0.460380 5.4:+46166568 MS.gene73917:CDS
GGAGGAGGAGGATGACAGAA+AGG 0.460604 5.4:+46166138 MS.gene73917:CDS
GGGTATAGAGTGGAAGAGTT+GGG 0.462213 5.4:+46167228 MS.gene73917:CDS
AAGCTGTTACTCATGAAAGA+AGG 0.464529 5.4:+46166479 MS.gene73917:CDS
CAGTGAGAAGGCAGAGTTTG+TGG 0.469456 5.4:-46166357 None:intergenic
TAACAGCTTCAAGGATGGCT+TGG 0.472218 5.4:-46166465 None:intergenic
TTCGACAACGGAGGAGGAGG+AGG 0.476647 5.4:+46166120 MS.gene73917:CDS
AGTGGTAGTGTTACTGTGAT+TGG 0.477368 5.4:-46165897 None:intergenic
ACATTAGTCGAGGAGGAAGT+AGG 0.480496 5.4:+46166904 MS.gene73917:CDS
TATCATGGAAGGGGCCCAAT+GGG 0.484349 5.4:+46166522 MS.gene73917:CDS
GACAACGGAGGAGGAGGAGG+AGG 0.484502 5.4:+46166123 MS.gene73917:CDS
GAGCTTTGTTCCTAACTCCT+TGG 0.485022 5.4:-46166878 None:intergenic
CACAAACTCTGCCTTCTCAC+TGG 0.485867 5.4:+46166358 MS.gene73917:CDS
TGGCTCAAGTGATATTGATT+CGG 0.487459 5.4:+46166230 MS.gene73917:CDS
GCACCTCCTTATGGCGGCAG+CGG 0.487716 5.4:+46166174 MS.gene73917:CDS
ATCGCGAAAAGAAGGCCTGC+CGG 0.491290 5.4:+46166567 MS.gene73917:CDS
TGAAGTTGTGGAAGTCATCT+TGG 0.492730 5.4:-46165985 None:intergenic
TGAAGAAGAAGATGGATCCA+TGG 0.495037 5.4:+46166078 MS.gene73917:CDS
TCACTTTCATCGCGAAAAGA+AGG 0.496428 5.4:+46166559 MS.gene73917:CDS
TATTGGGTTTATTTAACGAT+GGG 0.498402 5.4:+46167208 MS.gene73917:CDS
CCTCCGCTGCCGCCATAAGG+AGG 0.504163 5.4:-46166177 None:intergenic
CTCAAATTCGACAACGGAGG+AGG 0.505265 5.4:+46166114 MS.gene73917:CDS
AACGGGTGTGATTTCATCGT+TGG 0.506714 5.4:-46166020 None:intergenic
AGGCTGAGAGTATGTCTGTT+TGG 0.507765 5.4:-46166387 None:intergenic
CTGCTAAACGGCACCGATTG+TGG 0.507905 5.4:+46166331 MS.gene73917:CDS
GCCGTTTAGCAGAGTTTGGA+GGG 0.509680 5.4:-46166320 None:intergenic
TGGGTTTCCTATGAATAAAT+GGG 0.510723 5.4:+46167008 MS.gene73917:CDS
TTGAATGAGTGAAGCCCATT+GGG 0.514305 5.4:-46166536 None:intergenic
AGTGATATTGATTCGGCTCA+AGG 0.514796 5.4:+46166237 MS.gene73917:CDS
CACAAACAAGAGTCACCATC+TGG 0.516410 5.4:+46166210 MS.gene73917:CDS
TGCCGTTTAGCAGAGTTTGG+AGG 0.517244 5.4:-46166321 None:intergenic
TCTTCGTTGAAGCTTGTTCG+CGG 0.517579 5.4:+46166769 MS.gene73917:CDS
GAGTTTGTGGTGACCACAAT+CGG 0.519040 5.4:-46166344 None:intergenic
GTCAAGGAGAGGGGACCAAA+TGG 0.519529 5.4:-46165942 None:intergenic
TTGGGTGTGGAAGGGTTCAA+AGG 0.525480 5.4:+46167144 MS.gene73917:CDS
TGTGATTTCATCGTTGGTGA+GGG 0.527401 5.4:-46166014 None:intergenic
ATAACAACTTTGCTTGACAA+TGG 0.528206 5.4:-46167188 None:intergenic
ACAAAGCCTGCATCGTTGAT+GGG 0.528462 5.4:-46166928 None:intergenic
TGAAAGAAGGGTTCACATAG+TGG 0.529945 5.4:+46166492 MS.gene73917:CDS
GTAGGGCCCATCAACGATGC+AGG 0.535058 5.4:+46166922 MS.gene73917:CDS
TGAGTTGGACATGTCTCCCA+TGG 0.535777 5.4:-46166095 None:intergenic
TAAATGGGCTAGGAGTTTAG+TGG 0.535874 5.4:+46167023 MS.gene73917:CDS
AAAGGAGTTAACATAAGCTA+TGG 0.536826 5.4:+46167162 MS.gene73917:CDS
TCTTCCAGTTTCTTGAACAG+TGG 0.536983 5.4:-46166662 None:intergenic
TTATTGGGTTTATTTAACGA+TGG 0.539610 5.4:+46167207 MS.gene73917:CDS
AGCGATGTATGACTCGTTCG+AGG 0.539838 5.4:+46166984 MS.gene73917:CDS
GCCCTCCAAACTCTGCTAAA+CGG 0.540807 5.4:+46166319 MS.gene73917:CDS
GAGGAGGAGGATGACAGAAA+GGG 0.544247 5.4:+46166139 MS.gene73917:CDS
GTGGAAGTCATCTTGGTTGG+TGG 0.544732 5.4:-46165978 None:intergenic
AGTTTCTTGAACAGTGGCAA+AGG 0.546696 5.4:-46166656 None:intergenic
AAATCTAGATAACAAGGTCG+TGG 0.550244 5.4:+46166048 MS.gene73917:CDS
TTCCTATGAATAAATGGGCT+AGG 0.550588 5.4:+46167013 MS.gene73917:CDS
GAAGAAGAAGATGGATCCAT+GGG 0.550649 5.4:+46166079 MS.gene73917:CDS
GTGAAGAGGAGGAGCAAGAG+AGG 0.551124 5.4:+46167103 MS.gene73917:CDS
TCTATTGCTTCATTCTTGAA+CGG 0.552156 5.4:+46166853 MS.gene73917:CDS
CTCCATGTTGGTGCCGTGAG+TGG 0.552588 5.4:-46166272 None:intergenic
GAGGAAGATGAAGAAGAAGA+TGG 0.554434 5.4:+46166070 MS.gene73917:CDS
TTCATTCTTGAACGGTGCCA+AGG 0.558389 5.4:+46166861 MS.gene73917:CDS
TTTGGTGCGGTCCAGTGAGA+AGG 0.559073 5.4:-46166369 None:intergenic
CATTAGTCGAGGAGGAAGTA+GGG 0.559643 5.4:+46166905 MS.gene73917:CDS
GTGTGATTTCATCGTTGGTG+AGG 0.561242 5.4:-46166015 None:intergenic
AGAGTTGGGAAACAATAAGT+TGG 0.563779 5.4:+46167242 MS.gene73917:CDS
GACTTAGTGTCCTCCACTCA+CGG 0.564053 5.4:+46166259 MS.gene73917:CDS
ATTTAACGATGGGTATAGAG+TGG 0.564807 5.4:+46167218 MS.gene73917:CDS
TATTGACATAGACATGATCA+TGG 0.565364 5.4:+46165847 MS.gene73917:CDS
AAGAAATCCAAGTCAAGGAG+AGG 0.565976 5.4:-46165953 None:intergenic
AGCTGTTACTCATGAAAGAA+GGG 0.566179 5.4:+46166480 MS.gene73917:CDS
TGGAGAATTCTAGAAAATCT+GGG 0.566834 5.4:-46165877 None:intergenic
GTGGCCCAGTTGTACAAGAC+TGG 0.567811 5.4:+46167081 MS.gene73917:CDS
AAATTCGACAACGGAGGAGG+AGG 0.568207 5.4:+46166117 MS.gene73917:CDS
AATGGTGTCGTCATCAGAAG+TGG 0.570203 5.4:-46165924 None:intergenic
CTACATTGATGAAAAGTGAA+TGG 0.571536 5.4:-46166718 None:intergenic
TTTGCCACATCTTAGTTACC+GGG 0.572604 5.4:+46166822 MS.gene73917:CDS
GCAAGAGAGGTTCTCTTGGG+GGG 0.573233 5.4:+46167116 MS.gene73917:CDS
GTTGTGGAAGTCATCTTGGT+TGG 0.573299 5.4:-46165981 None:intergenic
GTGGACTTTGATATCATGGA+AGG 0.574432 5.4:+46166511 MS.gene73917:CDS
AAGCAATAGAATCTGAAGCC+CGG 0.574832 5.4:-46166840 None:intergenic
ATTGAATGAGTGAAGCCCAT+TGG 0.575421 5.4:-46166537 None:intergenic
CATGTCTTGAAGAAGCTGAA+AGG 0.576740 5.4:-46166407 None:intergenic
ATGAGTAACAGCTTCAAGGA+TGG 0.578715 5.4:-46166470 None:intergenic
AAATGAGCAAACTTGACGTA+AGG 0.579384 5.4:-46166433 None:intergenic
CCACTGAGCCCATTATTCGT+GGG 0.580098 5.4:-46167062 None:intergenic
GGACTTTGATATCATGGAAG+GGG 0.580763 5.4:+46166513 MS.gene73917:CDS
ATATCATGGAAGGGGCCCAA+TGG 0.582076 5.4:+46166521 MS.gene73917:CDS
AGAAACAAAGAACGTGGCAA+TGG 0.585187 5.4:+46166619 MS.gene73917:CDS
CTCCACTCACGGCACCAACA+TGG 0.585629 5.4:+46166270 MS.gene73917:CDS
GTGATGCGAAGATGTGGGCC+CGG 0.587438 5.4:-46166586 None:intergenic
GTTATCTAGATTTGGAGCAA+CGG 0.590909 5.4:-46166038 None:intergenic
CTGTTCAAGAAACTGGAAGA+AGG 0.593746 5.4:+46166665 MS.gene73917:CDS
GTTGTACAAGACTGGTGAAG+AGG 0.596068 5.4:+46167089 MS.gene73917:CDS
GTTGAAGCTTGTTCGCGGAG+AGG 0.596988 5.4:+46166774 MS.gene73917:CDS
GTACAAGACTGGTGAAGAGG+AGG 0.599329 5.4:+46167092 MS.gene73917:CDS
TCAATATCACTTGAGCCAGA+TGG 0.599918 5.4:-46166225 None:intergenic
ATGGGCTAGGAGTTTAGTGG+AGG 0.599983 5.4:+46167026 MS.gene73917:CDS
CCATAAGGAGGTGCACTAGA+CGG 0.601550 5.4:-46166165 None:intergenic
TAGCAGAGTTTGGAGGGCGT+CGG 0.602737 5.4:-46166314 None:intergenic
GAGCAAGAGAGGTTCTCTTG+GGG 0.606683 5.4:+46167114 MS.gene73917:CDS
AATGCAGTGATGCGAAGATG+TGG 0.609707 5.4:-46166592 None:intergenic
CATGTTGGTGCCGTGAGTGG+AGG 0.611646 5.4:-46166269 None:intergenic
TGGACTTTGATATCATGGAA+GGG 0.613948 5.4:+46166512 MS.gene73917:CDS
GTCATCAGAAGTGGGTGGAG+TGG 0.617810 5.4:-46165915 None:intergenic
CGCAGCGAGTCTCTCCATGT+TGG 0.618231 5.4:-46166284 None:intergenic
TGCTCCAAATCTAGATAACA+AGG 0.618396 5.4:+46166042 MS.gene73917:CDS
GAACATGGAGATTCATGACA+TGG 0.620847 5.4:+46165823 MS.gene73917:CDS
ATTGTATATGATCATGAACA+TGG 0.621187 5.4:+46165808 None:intergenic
TGAGGGAATCAATGAAGTTG+TGG 0.623812 5.4:-46165997 None:intergenic
TGGTGAAGAAATCCAAGTCA+AGG 0.627017 5.4:-46165958 None:intergenic
TTCACCAGTCTTGTACAACT+GGG 0.634954 5.4:-46167085 None:intergenic
AGAAATCCAAGTCAAGGAGA+GGG 0.637230 5.4:-46165952 None:intergenic
AAAAGTGAATGGTTGTCCAA+CGG 0.637307 5.4:-46166707 None:intergenic
TGTTAGGTTGGAAATCAAGA+CGG 0.641566 5.4:+46167265 MS.gene73917:CDS
TCTAGATAACAAGGTCGTGG+AGG 0.643866 5.4:+46166051 MS.gene73917:CDS
AGCAAGAGAGGTTCTCTTGG+GGG 0.646650 5.4:+46167115 MS.gene73917:CDS
ATGGTGTCGTCATCAGAAGT+GGG 0.650040 5.4:-46165923 None:intergenic
GAAATCCAAGTCAAGGAGAG+GGG 0.653641 5.4:-46165951 None:intergenic
GTGTCGTCATCAGAAGTGGG+TGG 0.657852 5.4:-46165920 None:intergenic
GAAGCCCGGTAACTAAGATG+TGG 0.661574 5.4:-46166826 None:intergenic
GCTCGTGACATTAGTCGAGG+AGG 0.670792 5.4:+46166897 MS.gene73917:CDS
CCCACGAATAATGGGCTCAG+TGG 0.676277 5.4:+46167062 MS.gene73917:CDS
AAAGCTCGTGACATTAGTCG+AGG 0.678929 5.4:+46166894 MS.gene73917:CDS
GAGAGTATGTCTGTTTGGTG+CGG 0.684249 5.4:-46166382 None:intergenic
ATGCAGTGATGCGAAGATGT+GGG 0.688648 5.4:-46166591 None:intergenic
GTCCAACTCAAATTCGACAA+CGG 0.690922 5.4:+46166108 MS.gene73917:CDS
TAAGGAGGTGCACTAGACGG+AGG 0.697542 5.4:-46166162 None:intergenic
TCTAGTGCACCTCCTTATGG+CGG 0.711985 5.4:+46166168 MS.gene73917:CDS
CAACTCAAATTCGACAACGG+AGG 0.712986 5.4:+46166111 MS.gene73917:CDS
TTGTCTAGAAACAAAGAACG+TGG 0.726054 5.4:+46166613 MS.gene73917:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTGGGTTTATTTAACGAT+GGG + chr5.4:46167208-46167227 MS.gene73917:CDS 25.0%
! TTATTGGGTTTATTTAACGA+TGG + chr5.4:46167207-46167226 MS.gene73917:CDS 25.0%
AAAGGAGTTAACATAAGCTA+TGG + chr5.4:46167162-46167181 MS.gene73917:CDS 30.0%
ATAACAACTTTGCTTGACAA+TGG - chr5.4:46167191-46167210 None:intergenic 30.0%
CTACATTGATGAAAAGTGAA+TGG - chr5.4:46166721-46166740 None:intergenic 30.0%
TATTGACATAGACATGATCA+TGG + chr5.4:46165847-46165866 MS.gene73917:CDS 30.0%
TCTATTGCTTCATTCTTGAA+CGG + chr5.4:46166853-46166872 MS.gene73917:CDS 30.0%
TGGGTTTCCTATGAATAAAT+GGG + chr5.4:46167008-46167027 MS.gene73917:CDS 30.0%
TTGTCAAGCAAAGTTGTTAT+TGG + chr5.4:46167191-46167210 MS.gene73917:CDS 30.0%
! CACTTTTCATCAATGTAGAT+TGG + chr5.4:46166723-46166742 MS.gene73917:CDS 30.0%
! TGGAGAATTCTAGAAAATCT+GGG - chr5.4:46165880-46165899 None:intergenic 30.0%
! TGTCAAGCAAAGTTGTTATT+GGG + chr5.4:46167192-46167211 MS.gene73917:CDS 30.0%
! TTGGAGAATTCTAGAAAATC+TGG - chr5.4:46165881-46165900 None:intergenic 30.0%
!!! TTAGTGGAGGAAGTATTTTT+AGG + chr5.4:46167039-46167058 MS.gene73917:CDS 30.0%
AAAAGTGAATGGTTGTCCAA+CGG - chr5.4:46166710-46166729 None:intergenic 35.0%
AAATCTAGATAACAAGGTCG+TGG + chr5.4:46166048-46166067 MS.gene73917:CDS 35.0%
AAATGAGCAAACTTGACGTA+AGG - chr5.4:46166436-46166455 None:intergenic 35.0%
AAGCTGTTACTCATGAAAGA+AGG + chr5.4:46166479-46166498 MS.gene73917:CDS 35.0%
AGAGTTGGGAAACAATAAGT+TGG + chr5.4:46167242-46167261 MS.gene73917:CDS 35.0%
AGCTGTTACTCATGAAAGAA+GGG + chr5.4:46166480-46166499 MS.gene73917:CDS 35.0%
ATTTAACGATGGGTATAGAG+TGG + chr5.4:46167218-46167237 MS.gene73917:CDS 35.0%
ATTTGCCACATCTTAGTTAC+CGG + chr5.4:46166821-46166840 MS.gene73917:CDS 35.0%
CTGGGTTTCCTATGAATAAA+TGG + chr5.4:46167007-46167026 MS.gene73917:CDS 35.0%
TGCTCCAAATCTAGATAACA+AGG + chr5.4:46166042-46166061 MS.gene73917:CDS 35.0%
TGGACTTTGATATCATGGAA+GGG + chr5.4:46166512-46166531 MS.gene73917:CDS 35.0%
TTGTCTAGAAACAAAGAACG+TGG + chr5.4:46166613-46166632 MS.gene73917:CDS 35.0%
TTTCATGAGTAACAGCTTCA+AGG - chr5.4:46166477-46166496 None:intergenic 35.0%
! ACGACCTTGTTATCTAGATT+TGG - chr5.4:46166049-46166068 None:intergenic 35.0%
! GTTATCTAGATTTGGAGCAA+CGG - chr5.4:46166041-46166060 None:intergenic 35.0%
! TGGCTCAAGTGATATTGATT+CGG + chr5.4:46166230-46166249 MS.gene73917:CDS 35.0%
! TTATCTAGATTTGGAGCAAC+GGG - chr5.4:46166040-46166059 None:intergenic 35.0%
! TTCCTATGAATAAATGGGCT+AGG + chr5.4:46167013-46167032 MS.gene73917:CDS 35.0%
!! ACAATAAGTTGGTGTTAGGT+TGG + chr5.4:46167253-46167272 MS.gene73917:CDS 35.0%
!! ATTTTTAGGCCCACGAATAA+TGG + chr5.4:46167053-46167072 MS.gene73917:CDS 35.0%
!! CATAGTGGACTTTGATATCA+TGG + chr5.4:46166507-46166526 MS.gene73917:CDS 35.0%
!! GGAAACAATAAGTTGGTGTT+AGG + chr5.4:46167249-46167268 MS.gene73917:CDS 35.0%
!! TGTTAGGTTGGAAATCAAGA+CGG + chr5.4:46167265-46167284 MS.gene73917:CDS 35.0%
!! TTTTTAGGCCCACGAATAAT+GGG + chr5.4:46167054-46167073 MS.gene73917:CDS 35.0%
AAGAAATCCAAGTCAAGGAG+AGG - chr5.4:46165956-46165975 None:intergenic 40.0%
AGAAACAAAGAACGTGGCAA+TGG + chr5.4:46166619-46166638 MS.gene73917:CDS 40.0%
AGAAATCCAAGTCAAGGAGA+GGG - chr5.4:46165955-46165974 None:intergenic 40.0%
AGTTTCTTGAACAGTGGCAA+AGG - chr5.4:46166659-46166678 None:intergenic 40.0%
ATGAGTAACAGCTTCAAGGA+TGG - chr5.4:46166473-46166492 None:intergenic 40.0%
ATTGAATGAGTGAAGCCCAT+TGG - chr5.4:46166540-46166559 None:intergenic 40.0%
CATGTCTTGAAGAAGCTGAA+AGG - chr5.4:46166410-46166429 None:intergenic 40.0%
CTCCTAGCCCATTTATTCAT+AGG - chr5.4:46167018-46167037 None:intergenic 40.0%
CTGTTCAAGAAACTGGAAGA+AGG + chr5.4:46166665-46166684 MS.gene73917:CDS 40.0%
GAACATGGAGATTCATGACA+TGG + chr5.4:46165823-46165842 MS.gene73917:CDS 40.0%
GAAGAAGAAGATGGATCCAT+GGG + chr5.4:46166079-46166098 MS.gene73917:CDS 40.0%
GAGGAAGATGAAGAAGAAGA+TGG + chr5.4:46166070-46166089 MS.gene73917:CDS 40.0%
GGACTTTGATATCATGGAAG+GGG + chr5.4:46166513-46166532 MS.gene73917:CDS 40.0%
GTCCAACTCAAATTCGACAA+CGG + chr5.4:46166108-46166127 MS.gene73917:CDS 40.0%
GTGGACTTTGATATCATGGA+AGG + chr5.4:46166511-46166530 MS.gene73917:CDS 40.0%
TCAATATCACTTGAGCCAGA+TGG - chr5.4:46166228-46166247 None:intergenic 40.0%
TCACTTTCATCGCGAAAAGA+AGG + chr5.4:46166559-46166578 MS.gene73917:CDS 40.0%
TCTTCCAGTTTCTTGAACAG+TGG - chr5.4:46166665-46166684 None:intergenic 40.0%
TGAAAGAAGGGTTCACATAG+TGG + chr5.4:46166492-46166511 MS.gene73917:CDS 40.0%
TGAAGAAGAAGATGGATCCA+TGG + chr5.4:46166078-46166097 MS.gene73917:CDS 40.0%
TGAAGTTGTGGAAGTCATCT+TGG - chr5.4:46165988-46166007 None:intergenic 40.0%
TGAGGGAATCAATGAAGTTG+TGG - chr5.4:46166000-46166019 None:intergenic 40.0%
TGGTGAAGAAATCCAAGTCA+AGG - chr5.4:46165961-46165980 None:intergenic 40.0%
TTGAATGAGTGAAGCCCATT+GGG - chr5.4:46166539-46166558 None:intergenic 40.0%
TTTGCCACATCTTAGTTACC+GGG + chr5.4:46166822-46166841 MS.gene73917:CDS 40.0%
TTTGCCACTGTTCAAGAAAC+TGG + chr5.4:46166658-46166677 MS.gene73917:CDS 40.0%
! AAGCAATAGAATCTGAAGCC+CGG - chr5.4:46166843-46166862 None:intergenic 40.0%
! ACTCTTGTTTGTGCCTGTTA+GGG - chr5.4:46166203-46166222 None:intergenic 40.0%
! AGTGGTAGTGTTACTGTGAT+TGG - chr5.4:46165900-46165919 None:intergenic 40.0%
! CGTCAAGTTTGCTCATTTCA+CGG + chr5.4:46166438-46166457 MS.gene73917:CDS 40.0%
! TGTGATTTCATCGTTGGTGA+GGG - chr5.4:46166017-46166036 None:intergenic 40.0%
! TTCACCAGTCTTGTACAACT+GGG - chr5.4:46167088-46167107 None:intergenic 40.0%
!! AGTGATATTGATTCGGCTCA+AGG + chr5.4:46166237-46166256 MS.gene73917:CDS 40.0%
!! TAAATGGGCTAGGAGTTTAG+TGG + chr5.4:46167023-46167042 MS.gene73917:CDS 40.0%
AAAGCTCGTGACATTAGTCG+AGG + chr5.4:46166894-46166913 MS.gene73917:CDS 45.0%
AACGGGTGTGATTTCATCGT+TGG - chr5.4:46166023-46166042 None:intergenic 45.0%
AATGCAGTGATGCGAAGATG+TGG - chr5.4:46166595-46166614 None:intergenic 45.0%
ACAAAGCCTGCATCGTTGAT+GGG - chr5.4:46166931-46166950 None:intergenic 45.0%
ACATTAGTCGAGGAGGAAGT+AGG + chr5.4:46166904-46166923 MS.gene73917:CDS 45.0%
ATGCAGTGATGCGAAGATGT+GGG - chr5.4:46166594-46166613 None:intergenic 45.0%
CAACTCAAATTCGACAACGG+AGG + chr5.4:46166111-46166130 MS.gene73917:CDS 45.0%
CACAAACAAGAGTCACCATC+TGG + chr5.4:46166210-46166229 MS.gene73917:CDS 45.0%
CATTAGTCGAGGAGGAAGTA+GGG + chr5.4:46166905-46166924 MS.gene73917:CDS 45.0%
CTCCGTTGTCGAATTTGAGT+TGG - chr5.4:46166113-46166132 None:intergenic 45.0%
GAAATCCAAGTCAAGGAGAG+GGG - chr5.4:46165954-46165973 None:intergenic 45.0%
GAGTTTGTGGTGACCACAAT+CGG - chr5.4:46166347-46166366 None:intergenic 45.0%
GGGTATAGAGTGGAAGAGTT+GGG + chr5.4:46167228-46167247 MS.gene73917:CDS 45.0%
GTGTGATTTCATCGTTGGTG+AGG - chr5.4:46166018-46166037 None:intergenic 45.0%
GTTGTACAAGACTGGTGAAG+AGG + chr5.4:46167089-46167108 MS.gene73917:CDS 45.0%
GTTGTGGAAGTCATCTTGGT+TGG - chr5.4:46165984-46166003 None:intergenic 45.0%
TAACAGCTTCAAGGATGGCT+TGG - chr5.4:46166468-46166487 None:intergenic 45.0%
TCTAGATAACAAGGTCGTGG+AGG + chr5.4:46166051-46166070 MS.gene73917:CDS 45.0%
TGGGTATAGAGTGGAAGAGT+TGG + chr5.4:46167227-46167246 MS.gene73917:CDS 45.0%
! AGGCTGAGAGTATGTCTGTT+TGG - chr5.4:46166390-46166409 None:intergenic 45.0%
! AGGCTTTGTGGGTTTGTTCA+TGG + chr5.4:46166942-46166961 MS.gene73917:CDS 45.0%
! ATCAACGATGCAGGCTTTGT+GGG + chr5.4:46166931-46166950 MS.gene73917:CDS 45.0%
! CTTCACCAGTCTTGTACAAC+TGG - chr5.4:46167089-46167108 None:intergenic 45.0%
! CTTCTGATGACGACACCATT+TGG + chr5.4:46165927-46165946 MS.gene73917:CDS 45.0%
! GACTCTTGTTTGTGCCTGTT+AGG - chr5.4:46166204-46166223 None:intergenic 45.0%
! GAGAGTATGTCTGTTTGGTG+CGG - chr5.4:46166385-46166404 None:intergenic 45.0%
! TCTTCGTTGAAGCTTGTTCG+CGG + chr5.4:46166769-46166788 MS.gene73917:CDS 45.0%
! TTCATTCTTGAACGGTGCCA+AGG + chr5.4:46166861-46166880 MS.gene73917:CDS 45.0%
!! AATGGTGTCGTCATCAGAAG+TGG - chr5.4:46165927-46165946 None:intergenic 45.0%
!! ATGGTGTCGTCATCAGAAGT+GGG - chr5.4:46165926-46165945 None:intergenic 45.0%
!! GAGCTTTGTTCCTAACTCCT+TGG - chr5.4:46166881-46166900 None:intergenic 45.0%
AAATTCGACAACGGAGGAGG+AGG + chr5.4:46166117-46166136 MS.gene73917:CDS 50.0%
AGCAAGAGAGGTTCTCTTGG+GGG + chr5.4:46167115-46167134 MS.gene73917:CDS 50.0%
AGCGATGTATGACTCGTTCG+AGG + chr5.4:46166984-46167003 MS.gene73917:CDS 50.0%
AGGAGCAAGAGAGGTTCTCT+TGG + chr5.4:46167112-46167131 MS.gene73917:CDS 50.0%
ATATCATGGAAGGGGCCCAA+TGG + chr5.4:46166521-46166540 MS.gene73917:CDS 50.0%
ATGTATGACTCGTTCGAGGC+TGG + chr5.4:46166988-46167007 MS.gene73917:CDS 50.0%
CACAAACTCTGCCTTCTCAC+TGG + chr5.4:46166358-46166377 MS.gene73917:CDS 50.0%
CACAAAGCCTGCATCGTTGA+TGG - chr5.4:46166932-46166951 None:intergenic 50.0%
CAGTGAGAAGGCAGAGTTTG+TGG - chr5.4:46166360-46166379 None:intergenic 50.0%
CATCAACGATGCAGGCTTTG+TGG + chr5.4:46166930-46166949 MS.gene73917:CDS 50.0%
CCACTGAGCCCATTATTCGT+GGG - chr5.4:46167065-46167084 None:intergenic 50.0%
CTCAAATTCGACAACGGAGG+AGG + chr5.4:46166114-46166133 MS.gene73917:CDS 50.0%
GAAGCCCGGTAACTAAGATG+TGG - chr5.4:46166829-46166848 None:intergenic 50.0%
GACTTAGTGTCCTCCACTCA+CGG + chr5.4:46166259-46166278 MS.gene73917:CDS 50.0%
GAGCAAGAGAGGTTCTCTTG+GGG + chr5.4:46167114-46167133 MS.gene73917:CDS 50.0%
GAGGAGGAGGATGACAGAAA+GGG + chr5.4:46166139-46166158 MS.gene73917:CDS 50.0%
GCCCTCCAAACTCTGCTAAA+CGG + chr5.4:46166319-46166338 MS.gene73917:CDS 50.0%
GCCGTTTAGCAGAGTTTGGA+GGG - chr5.4:46166323-46166342 None:intergenic 50.0%
GGAGCAAGAGAGGTTCTCTT+GGG + chr5.4:46167113-46167132 MS.gene73917:CDS 50.0%
GTACAAGACTGGTGAAGAGG+AGG + chr5.4:46167092-46167111 MS.gene73917:CDS 50.0%
GTGGAAGTCATCTTGGTTGG+TGG - chr5.4:46165981-46166000 None:intergenic 50.0%
TATCATGGAAGGGGCCCAAT+GGG + chr5.4:46166522-46166541 MS.gene73917:CDS 50.0%
TCTAGTGCACCTCCTTATGG+CGG + chr5.4:46166168-46166187 MS.gene73917:CDS 50.0%
TGTATGACTCGTTCGAGGCT+GGG + chr5.4:46166989-46167008 MS.gene73917:CDS 50.0%
TTGGGTGTGGAAGGGTTCAA+AGG + chr5.4:46167144-46167163 MS.gene73917:CDS 50.0%
! CCATAAGGAGGTGCACTAGA+CGG - chr5.4:46166168-46166187 None:intergenic 50.0%
! GGCTGCTTTCTGCTTCTGTT+TGG + chr5.4:46167286-46167305 MS.gene73917:CDS 50.0%
! TGAGTTGGACATGTCTCCCA+TGG - chr5.4:46166098-46166117 None:intergenic 50.0%
! TGCCGTTTAGCAGAGTTTGG+AGG - chr5.4:46166324-46166343 None:intergenic 50.0%
! TTGAACGGTGCCAAGGAGTT+AGG + chr5.4:46166868-46166887 MS.gene73917:CDS 50.0%
!! ACAGCTTTTGCTGCTTCCGT+TGG + chr5.4:46166691-46166710 MS.gene73917:CDS 50.0%
!! ATGGGCTAGGAGTTTAGTGG+AGG + chr5.4:46167026-46167045 MS.gene73917:CDS 50.0%
ATCGCGAAAAGAAGGCCTGC+CGG + chr5.4:46166567-46166586 MS.gene73917:CDS 55.0%
CCGTCTAGTGCACCTCCTTA+TGG + chr5.4:46166165-46166184 MS.gene73917:CDS 55.0%
GCAAGAGAGGTTCTCTTGGG+GGG + chr5.4:46167116-46167135 MS.gene73917:CDS 55.0%
GCCACTGAGCCCATTATTCG+TGG - chr5.4:46167066-46167085 None:intergenic 55.0%
GCTCGTGACATTAGTCGAGG+AGG + chr5.4:46166897-46166916 MS.gene73917:CDS 55.0%
GGAGGAGGAGGATGACAGAA+AGG + chr5.4:46166138-46166157 MS.gene73917:CDS 55.0%
GTCAAGGAGAGGGGACCAAA+TGG - chr5.4:46165945-46165964 None:intergenic 55.0%
GTCATCAGAAGTGGGTGGAG+TGG - chr5.4:46165918-46165937 None:intergenic 55.0%
GTGAAGAGGAGGAGCAAGAG+AGG + chr5.4:46167103-46167122 MS.gene73917:CDS 55.0%
GTGGCCCAGTTGTACAAGAC+TGG + chr5.4:46167081-46167100 MS.gene73917:CDS 55.0%
GTGTCGTCATCAGAAGTGGG+TGG - chr5.4:46165923-46165942 None:intergenic 55.0%
TTCTCTTGGGGGGAGTGGTT+GGG + chr5.4:46167126-46167145 MS.gene73917:CDS 55.0%
TTGGTCCCCTCTCCTTGACT+TGG + chr5.4:46165946-46165965 MS.gene73917:CDS 55.0%
! GTTGAAGCTTGTTCGCGGAG+AGG + chr5.4:46166774-46166793 MS.gene73917:CDS 55.0%
! TAAGGAGGTGCACTAGACGG+AGG - chr5.4:46166165-46166184 None:intergenic 55.0%
! TAGCAGAGTTTGGAGGGCGT+CGG - chr5.4:46166317-46166336 None:intergenic 55.0%
! TTTGGTGCGGTCCAGTGAGA+AGG - chr5.4:46166372-46166391 None:intergenic 55.0%
!! CCCACGAATAATGGGCTCAG+TGG + chr5.4:46167062-46167081 MS.gene73917:CDS 55.0%
!! CGGTGCCGTTTAGCAGAGTT+TGG - chr5.4:46166327-46166346 None:intergenic 55.0%
!! CTGCTAAACGGCACCGATTG+TGG + chr5.4:46166331-46166350 MS.gene73917:CDS 55.0%
AGAGGTTCTCTTGGGGGGAG+TGG + chr5.4:46167121-46167140 MS.gene73917:CDS 60.0%
CGCAGCGAGTCTCTCCATGT+TGG - chr5.4:46166287-46166306 None:intergenic 60.0%
CTCCACTCACGGCACCAACA+TGG + chr5.4:46166270-46166289 MS.gene73917:CDS 60.0%
GCTTGTTCGCGGAGAGGCTT+TGG + chr5.4:46166780-46166799 MS.gene73917:CDS 60.0%
GGGAGTGGTTGGGTGTGGAA+GGG + chr5.4:46167136-46167155 MS.gene73917:CDS 60.0%
GTAGGGCCCATCAACGATGC+AGG + chr5.4:46166922-46166941 MS.gene73917:CDS 60.0%
GTGATGCGAAGATGTGGGCC+CGG - chr5.4:46166589-46166608 None:intergenic 60.0%
GTTCTCTTGGGGGGAGTGGT+TGG + chr5.4:46167125-46167144 MS.gene73917:CDS 60.0%
TCGCGAAAAGAAGGCCTGCC+GGG + chr5.4:46166568-46166587 MS.gene73917:CDS 60.0%
TTCGACAACGGAGGAGGAGG+AGG + chr5.4:46166120-46166139 MS.gene73917:CDS 60.0%
!! CATGTTGGTGCCGTGAGTGG+AGG - chr5.4:46166272-46166291 None:intergenic 60.0%
!! CTCCATGTTGGTGCCGTGAG+TGG - chr5.4:46166275-46166294 None:intergenic 60.0%
AACGGAGGAGGAGGAGGAGG+AGG + chr5.4:46166126-46166145 MS.gene73917:CDS 65.0%
GACAACGGAGGAGGAGGAGG+AGG + chr5.4:46166123-46166142 MS.gene73917:CDS 65.0%
GCACCTCCTTATGGCGGCAG+CGG + chr5.4:46166174-46166193 MS.gene73917:CDS 65.0%
GGGGAGTGGTTGGGTGTGGA+AGG + chr5.4:46167135-46167154 MS.gene73917:CDS 65.0%
TTGGGGGGAGTGGTTGGGTG+TGG + chr5.4:46167131-46167150 MS.gene73917:CDS 65.0%
CCTCCGCTGCCGCCATAAGG+AGG - chr5.4:46166180-46166199 None:intergenic 70.0%
CCTCCTTATGGCGGCAGCGG+AGG + chr5.4:46166177-46166196 MS.gene73917:CDS 70.0%
TGCGAAGATGTGGGCCCGGC+AGG - chr5.4:46166585-46166604 None:intergenic 70.0%
GCGGCAGCGGAGGCCCTAAC+AGG + chr5.4:46166187-46166206 MS.gene73917:CDS 75.0%
GGGCCTCCGCTGCCGCCATA+AGG - chr5.4:46166183-46166202 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 46165815 46167341 46165815 ID=MS.gene73917
chr5.4 mRNA 46165815 46167341 46165815 ID=MS.gene73917.t1;Parent=MS.gene73917
chr5.4 exon 46165815 46167341 46165815 ID=MS.gene73917.t1.exon1;Parent=MS.gene73917.t1
chr5.4 CDS 46165815 46167341 46165815 ID=cds.MS.gene73917.t1;Parent=MS.gene73917.t1
Gene Sequence

>MS.gene73917

ATGATCATGAACATGGAGATTCATGACATGGATATTGACATAGACATGATCATGGATGTTGTCCCAGATTTTCTAGAATTCTCCAATCACAGTAACACTACCACTCCACCCACTTCTGATGACGACACCATTTGGTCCCCTCTCCTTGACTTGGATTTCTTCACCACCAACCAAGATGACTTCCACAACTTCATTGATTCCCTCACCAACGATGAAATCACACCCGTTGCTCCAAATCTAGATAACAAGGTCGTGGAGGAAGATGAAGAAGAAGATGGATCCATGGGAGACATGTCCAACTCAAATTCGACAACGGAGGAGGAGGAGGAGGAGGATGACAGAAAGGGCCTCCGTCTAGTGCACCTCCTTATGGCGGCAGCGGAGGCCCTAACAGGCACAAACAAGAGTCACCATCTGGCTCAAGTGATATTGATTCGGCTCAAGGACTTAGTGTCCTCCACTCACGGCACCAACATGGAGAGACTCGCTGCGTATTTTACCGACGCCCTCCAAACTCTGCTAAACGGCACCGATTGTGGTCACCACAAACTCTGCCTTCTCACTGGACCGCACCAAACAGACATACTCTCAGCCTTTCAGCTTCTTCAAGACATGTCTCCTTACGTCAAGTTTGCTCATTTCACGGCAAACCAAGCCATCCTTGAAGCTGTTACTCATGAAAGAAGGGTTCACATAGTGGACTTTGATATCATGGAAGGGGCCCAATGGGCTTCACTCATTCAATCACTTTCATCGCGAAAAGAAGGCCTGCCGGGCCCACATCTTCGCATCACTGCATTGTCTAGAAACAAAGAACGTGGCAATGGACGATCAAGATCGTCCTTTGCCACTGTTCAAGAAACTGGAAGAAGGTTAACAGCTTTTGCTGCTTCCGTTGGACAACCATTCACTTTTCATCAATGTAGATTGGAATCAGACGAAACTTTTCGAACATCTTCGTTGAAGCTTGTTCGCGGAGAGGCTTTGGTGTTCAACTGTGTCATGCATTTGCCACATCTTAGTTACCGGGCTTCAGATTCTATTGCTTCATTCTTGAACGGTGCCAAGGAGTTAGGAACAAAGCTCGTGACATTAGTCGAGGAGGAAGTAGGGCCCATCAACGATGCAGGCTTTGTGGGTTTGTTCATGGATTCATTGCATCGTTATTCAGCGATGTATGACTCGTTCGAGGCTGGGTTTCCTATGAATAAATGGGCTAGGAGTTTAGTGGAGGAAGTATTTTTAGGCCCACGAATAATGGGCTCAGTGGCCCAGTTGTACAAGACTGGTGAAGAGGAGGAGCAAGAGAGGTTCTCTTGGGGGGAGTGGTTGGGTGTGGAAGGGTTCAAAGGAGTTAACATAAGCTATGGTAACCATTGTCAAGCAAAGTTGTTATTGGGTTTATTTAACGATGGGTATAGAGTGGAAGAGTTGGGAAACAATAAGTTGGTGTTAGGTTGGAAATCAAGACGGCTGCTTTCTGCTTCTGTTTGGACTTGTTTGTCAGAGATTGAATATGATTTTTAA
Protein sequence

>MS.gene73917.t1

MIMNMEIHDMDIDIDMIMDVVPDFLEFSNHSNTTTPPTSDDDTIWSPLLDLDFFTTNQDDFHNFIDSLTNDEITPVAPNLDNKVVEEDEEEDGSMGDMSNSNSTTEEEEEEDDRKGLRLVHLLMAAAEALTGTNKSHHLAQVILIRLKDLVSSTHGTNMERLAAYFTDALQTLLNGTDCGHHKLCLLTGPHQTDILSAFQLLQDMSPYVKFAHFTANQAILEAVTHERRVHIVDFDIMEGAQWASLIQSLSSRKEGLPGPHLRITALSRNKERGNGRSRSSFATVQETGRRLTAFAASVGQPFTFHQCRLESDETFRTSSLKLVRGEALVFNCVMHLPHLSYRASDSIASFLNGAKELGTKLVTLVEEEVGPINDAGFVGLFMDSLHRYSAMYDSFEAGFPMNKWARSLVEEVFLGPRIMGSVAQLYKTGEEEEQERFSWGEWLGVEGFKGVNISYGNHCQAKLLLGLFNDGYRVEELGNNKLVLGWKSRRLLSASVWTCLSEIEYDF