AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73953.t1 MTR_5g061890 64.327 342 89 5 1 310 1 341 1.35e-141 407
MS.gene73953.t1 MTR_5g060780 64.327 342 89 5 1 310 1 341 1.26e-140 404
MS.gene73953.t1 MTR_5g460920 64.327 342 89 5 1 310 1 341 7.09e-140 402
MS.gene73953.t1 MTR_5g060770 74.910 279 26 4 33 310 7 242 1.35e-131 380
MS.gene73953.t1 MTR_5g460910 64.236 288 36 6 25 310 15 237 5.62e-107 317
MS.gene73953.t1 MTR_5g060630 49.735 378 122 8 1 311 1 377 1.14e-104 317
MS.gene73953.t1 MTR_5g460740 49.735 378 122 8 1 311 1 377 1.14e-104 317
MS.gene73953.t1 MTR_5g061220 48.642 405 115 14 1 321 8 403 4.63e-103 313
MS.gene73953.t1 MTR_1g058210 46.366 399 153 14 1 348 1 389 5.05e-92 283
MS.gene73953.t1 MTR_2g024430 44.476 353 133 15 4 310 12 347 3.46e-76 243
MS.gene73953.t1 MTR_5g040880 43.155 336 141 11 8 304 7 331 1.52e-71 231
MS.gene73953.t1 MTR_2g006270 42.151 344 142 13 5 304 6 336 5.83e-67 218
MS.gene73953.t1 MTR_5g040740 43.027 337 128 13 5 294 5 324 1.13e-66 216
MS.gene73953.t1 MTR_5g082140 38.133 375 173 11 5 333 5 366 4.97e-66 216
MS.gene73953.t1 MTR_2g006380 39.118 340 151 11 6 304 14 338 2.22e-62 206
MS.gene73953.t1 MTR_3g073420 34.626 361 169 11 30 337 63 409 8.01e-48 170
MS.gene73953.t1 MTR_5g062960 54.545 143 60 3 197 338 1 139 5.11e-41 143
MS.gene73953.t1 MTR_1g064430 31.008 387 174 15 11 310 26 406 1.86e-36 139
MS.gene73953.t1 MTR_1g064430 31.008 387 174 15 11 310 39 419 2.05e-36 139
MS.gene73953.t1 MTR_2g094570 30.108 372 176 14 11 304 10 375 1.49e-35 137
MS.gene73953.t1 MTR_2g094570 30.108 372 176 14 11 304 10 375 1.53e-35 137
MS.gene73953.t1 MTR_2g094570 30.108 372 176 14 11 304 66 431 2.20e-35 137
MS.gene73953.t1 MTR_1g094960 31.594 345 166 11 24 309 55 388 2.92e-35 135
MS.gene73953.t1 MTR_4g058930 29.517 393 188 14 1 309 1 388 1.80e-33 131
MS.gene73953.t1 MTR_4g058930 29.517 393 188 14 1 309 1 388 1.95e-33 131
MS.gene73953.t1 MTR_5g062970 43.005 193 71 6 12 166 38 229 7.57e-32 120
MS.gene73953.t1 MTR_7g101280 30.058 346 170 14 31 310 74 413 2.08e-30 122
MS.gene73953.t1 MTR_0041s0110 30.058 346 170 14 31 310 74 413 2.08e-30 122
MS.gene73953.t1 MTR_2g005240 26.425 386 210 13 9 338 20 387 4.39e-27 112
MS.gene73953.t1 MTR_7g062540 28.772 285 181 8 35 304 67 344 2.36e-25 107
MS.gene73953.t1 MTR_4g021580 28.920 287 178 9 35 304 72 349 1.14e-24 105
MS.gene73953.t1 MTR_4g021580 28.920 287 178 9 35 304 72 349 1.26e-24 105
MS.gene73953.t1 MTR_2g014770 25.648 347 212 11 2 304 43 387 2.51e-24 104
MS.gene73953.t1 MTR_8g100050 27.815 302 193 8 25 309 103 396 2.81e-24 104
MS.gene73953.t1 MTR_8g100050 27.815 302 193 8 25 309 103 396 3.37e-24 104
MS.gene73953.t1 MTR_2g014770 25.648 347 212 11 2 304 43 387 3.74e-24 103
MS.gene73953.t1 MTR_2g018690 27.557 352 179 16 12 304 23 357 4.17e-24 103
MS.gene73953.t1 MTR_7g101275 30.325 277 170 11 31 289 35 306 4.39e-24 102
MS.gene73953.t1 MTR_3g064050 27.508 309 175 11 26 304 71 360 2.69e-22 98.6
MS.gene73953.t1 MTR_5g076270 28.479 309 172 12 26 304 69 358 3.29e-22 98.6
MS.gene73953.t1 MTR_8g079492 27.221 349 183 16 12 304 23 356 3.91e-22 98.2
MS.gene73953.t1 MTR_8g079492 27.221 349 183 16 12 304 23 356 4.33e-22 98.2
MS.gene73953.t1 MTR_4g124900 25.439 342 213 8 9 309 20 360 1.93e-21 96.3
MS.gene73953.t1 MTR_4g124900 25.439 342 213 8 9 309 20 360 2.05e-21 96.3
MS.gene73953.t1 MTR_2g043250 26.936 297 198 6 31 309 65 360 5.50e-21 94.7
MS.gene73953.t1 MTR_8g027440 26.531 343 195 13 9 304 26 358 4.34e-20 92.0
MS.gene73953.t1 MTR_8g079492 28.720 289 165 12 42 304 1 274 4.81e-20 92.0
MS.gene73953.t1 MTR_1g024025 28.716 296 181 12 31 304 89 376 1.88e-19 90.5
MS.gene73953.t1 MTR_2g093740 27.333 300 189 11 30 304 103 398 9.71e-19 87.8
MS.gene73953.t1 MTR_2g093740 27.333 300 189 11 30 304 103 398 1.13e-18 87.8
MS.gene73953.t1 MTR_2g093740 27.333 300 189 11 30 304 103 398 1.35e-18 87.4
MS.gene73953.t1 MTR_8g446900 26.159 302 182 11 35 313 14 297 3.19e-18 85.1
MS.gene73953.t1 MTR_4g088210 24.269 342 207 12 12 304 38 376 2.29e-17 84.0
MS.gene73953.t1 MTR_4g088210 24.269 342 207 12 12 304 38 376 2.73e-17 83.6
MS.gene73953.t1 MTR_8g101360 24.561 342 212 8 7 304 26 365 3.37e-17 83.6
MS.gene73953.t1 MTR_4g060460 26.019 319 176 12 26 304 98 396 6.19e-17 82.4
MS.gene73953.t1 MTR_4g060460 26.019 319 176 12 26 304 98 396 7.35e-17 82.4
MS.gene73953.t1 MTR_2g093740 28.511 235 149 8 85 304 38 268 2.31e-16 80.5
MS.gene73953.t1 MTR_2g093740 28.511 235 149 8 85 304 38 268 3.04e-16 80.5
MS.gene73953.t1 MTR_2g093740 26.198 313 189 12 30 304 103 411 8.30e-16 79.0
MS.gene73953.t1 MTR_2g093740 26.198 313 189 12 30 304 103 411 1.39e-15 78.6
MS.gene73953.t1 MTR_2g093740 26.198 313 189 12 30 304 103 411 1.52e-15 78.2
MS.gene73953.t1 MTR_2g093740 27.016 248 149 9 85 304 38 281 2.33e-13 71.6
MS.gene73953.t1 MTR_2g093740 27.016 248 149 9 85 304 38 281 3.01e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73953.t1 AT1G77850 31.063 367 179 14 8 304 15 377 9.15e-45 162
MS.gene73953.t1 AT1G77850 31.063 367 179 14 8 304 15 377 9.52e-45 162
MS.gene73953.t1 AT4G30080 31.367 373 170 15 11 304 18 383 3.27e-37 142
MS.gene73953.t1 AT2G28350 29.427 384 181 17 11 309 9 387 6.07e-34 132
MS.gene73953.t1 AT2G28350 29.427 384 181 17 11 309 9 387 6.29e-34 132
MS.gene73953.t1 AT2G46530 30.952 294 169 11 31 304 71 350 9.28e-29 117
MS.gene73953.t1 AT2G46530 30.952 294 169 11 31 304 92 371 1.14e-28 117
MS.gene73953.t1 AT3G61830 26.724 348 190 12 8 304 21 354 8.43e-27 111
MS.gene73953.t1 AT3G61830 29.592 294 173 11 31 304 24 303 8.45e-27 111
MS.gene73953.t1 AT1G35520 29.677 310 182 13 35 326 80 371 5.22e-26 109
MS.gene73953.t1 AT1G34390 26.966 356 185 13 9 309 24 359 8.41e-26 108
MS.gene73953.t1 AT1G59750 27.353 340 192 11 12 304 22 353 1.51e-25 108
MS.gene73953.t1 AT1G59750 27.353 340 192 11 12 304 22 353 1.63e-25 108
MS.gene73953.t1 AT1G59750 27.353 340 192 11 12 304 22 353 1.68e-25 108
MS.gene73953.t1 AT1G59750 27.353 340 192 11 12 304 22 353 1.68e-25 108
MS.gene73953.t1 AT4G23980 28.000 300 193 8 31 313 62 355 3.82e-25 107
MS.gene73953.t1 AT4G23980 28.000 300 193 8 31 313 62 355 4.05e-25 107
MS.gene73953.t1 AT1G34410 30.033 303 171 13 35 313 80 365 9.40e-25 105
MS.gene73953.t1 AT1G34310 27.933 358 189 15 9 313 24 365 2.17e-24 104
MS.gene73953.t1 AT1G35240 29.568 301 175 12 35 313 80 365 2.98e-24 104
MS.gene73953.t1 AT1G30330 26.703 367 186 15 2 304 10 357 3.28e-24 104
MS.gene73953.t1 AT1G30330 26.836 354 179 14 12 304 25 359 4.63e-24 103
MS.gene73953.t1 AT1G30330 26.836 354 179 14 12 304 25 359 4.63e-24 103
MS.gene73953.t1 AT2G33860 28.487 337 187 15 31 331 96 414 8.03e-24 103
MS.gene73953.t1 AT5G37020 26.271 354 181 15 12 304 22 356 9.40e-24 103
MS.gene73953.t1 AT5G37020 26.271 354 181 15 12 304 22 356 1.20e-23 102
MS.gene73953.t1 AT5G62000 26.821 302 177 9 35 309 114 398 3.01e-23 101
MS.gene73953.t1 AT5G62000 26.821 302 177 9 35 309 114 398 3.19e-23 101
MS.gene73953.t1 AT5G62000 26.821 302 177 9 35 309 114 398 3.19e-23 101
MS.gene73953.t1 AT5G62000 26.821 302 177 9 35 309 114 398 3.19e-23 101
MS.gene73953.t1 AT5G62000 26.821 302 177 9 35 309 114 398 3.19e-23 101
MS.gene73953.t1 AT2G46530 30.709 254 146 10 67 304 24 263 6.18e-23 100
MS.gene73953.t1 AT1G35540 26.822 343 190 14 9 304 24 352 7.26e-23 100
MS.gene73953.t1 AT2G33860 28.519 270 156 12 85 331 2 257 1.56e-20 92.8
MS.gene73953.t1 AT5G60450 25.000 352 200 13 9 304 62 405 1.79e-19 90.1
MS.gene73953.t1 AT5G60450 25.000 352 200 13 9 304 38 381 2.08e-19 90.1
MS.gene73953.t1 AT1G34170 27.666 347 184 15 9 304 22 352 2.27e-19 89.4
MS.gene73953.t1 AT1G19850 27.660 282 191 6 35 304 108 388 1.65e-18 87.4
MS.gene73953.t1 AT1G19850 27.660 282 191 6 35 304 97 377 1.70e-18 87.4
MS.gene73953.t1 AT5G20730 25.507 345 210 11 9 309 21 362 2.04e-18 87.4
MS.gene73953.t1 AT5G20730 25.507 345 210 11 9 309 21 362 2.18e-18 87.0
MS.gene73953.t1 AT5G20730 25.797 345 208 12 9 309 21 361 3.31e-18 86.7
MS.gene73953.t1 AT1G19220 25.874 286 200 5 35 309 76 360 1.11e-17 85.1
MS.gene73953.t1 AT5G37020 28.571 245 136 10 85 304 31 261 1.88e-17 84.0
MS.gene73953.t1 AT1G34170 27.089 347 160 17 9 304 22 326 3.16e-13 70.9
MS.gene73953.t1 AT1G34170 27.089 347 160 17 9 304 22 326 4.09e-13 70.9

Find 80 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCCATCTTCGTCTTCT+TGG 0.210312 5.2:+51000784 None:intergenic
CCTGAAAACAACAGAATCTT+TGG 0.226595 5.2:+51000539 None:intergenic
ATTCTTCAAATGTCCCTAAT+TGG 0.230817 5.2:-51000188 MS.gene73953:CDS
ATGAAGATTGGTTGGGAATC+TGG 0.311617 5.2:-51000303 MS.gene73953:CDS
CGTAGGTGCAACCCCATTCT+TGG 0.311998 5.2:+51000622 None:intergenic
CTTTCACCCGCTTCAGCTGC+AGG 0.324014 5.2:+51000465 None:intergenic
ACCTCAAGAATGCGCCAATT+AGG 0.328543 5.2:+51000174 None:intergenic
GGCAACAAGTCAGCCGATTT+AGG 0.332336 5.2:+50999350 None:intergenic
TCCATCTTCGTCTTCTTGGC+CGG 0.332884 5.2:+51000788 None:intergenic
CTGCTCCTCACTCCGGTAAC+TGG 0.333359 5.2:-51000840 MS.gene73953:CDS
GACTTCTCCAAGTCAAGCTT+TGG 0.342525 5.2:+51000675 None:intergenic
ATCTTTGGAACCTTGGCATC+GGG 0.343346 5.2:+51001035 None:intergenic
CCTCAAGAATGCGCCAATTA+GGG 0.362876 5.2:+51000175 None:intergenic
CCATCTTCGTCTTCTTGGCC+GGG 0.390410 5.2:+51000789 None:intergenic
GGAGGTTCTTGGCCAGTTAC+CGG 0.390862 5.2:+51000828 None:intergenic
GCCATGCCTCTGCCTGAGCC+AGG 0.396403 5.2:-51001095 MS.gene73953:CDS
AGATTCCCAACCAATCTTCA+TGG 0.412679 5.2:+51000305 None:intergenic
GTACTGGCTGGTCTCACTTT+CGG 0.420748 5.2:-51000581 MS.gene73953:CDS
CAGTAGGAGGAGGAGGTTCT+TGG 0.423692 5.2:+51000817 None:intergenic
CGACCTTAAAAGCCAAGAAT+GGG 0.432454 5.2:-51000634 MS.gene73953:CDS
CCTCCTCCTACTGTTGTTCC+CGG 0.432625 5.2:-51000807 MS.gene73953:CDS
GCCGTGTCCACCCTAAAATA+TGG 0.440684 5.2:-51001073 MS.gene73953:CDS
GGGTGGACACGGCCTGGCTC+AGG 0.447654 5.2:+51001083 None:intergenic
ATACACTGGCAGTCCTAAAT+CGG 0.448630 5.2:-50999363 MS.gene73953:CDS
ATTATCCGACAGCTAGGAAT+TGG 0.450128 5.2:-51000365 MS.gene73953:CDS
TCTGCTGGAAAGATATTAGT+TGG 0.452740 5.2:-51000510 MS.gene73953:CDS
CCGCACTCTCAGTTAAATTC+CGG 0.457010 5.2:+51000439 None:intergenic
GATCTTTCCAAAGCTTGACT+TGG 0.477680 5.2:-51000682 MS.gene73953:CDS
TACCGGAGTGAGGAGCAGTT+TGG 0.479172 5.2:+51000845 None:intergenic
AACTTCCCTGCAGCTGAAGC+GGG 0.481484 5.2:-51000471 MS.gene73953:CDS
ATCAGGAAAAGTAGAATGGC+AGG 0.482347 5.2:-50999416 MS.gene73953:CDS
TTATCATAGGTTAACTGGGA+TGG 0.485250 5.2:-50999536 MS.gene73953:intron
ACAGTAGAGATTGTTCCCTT+AGG 0.494524 5.2:+51000219 None:intergenic
ACACGGCCTGGCTCAGGCAG+AGG 0.496843 5.2:+51001089 None:intergenic
ACAAGGGTGATGGTTTCATC+AGG 0.497114 5.2:-50999433 MS.gene73953:CDS
GTAGATGATGCCATGAAGAT+TGG 0.497274 5.2:-51000315 MS.gene73953:CDS
CCCTAATTGGCGCATTCTTG+AGG 0.497342 5.2:-51000175 MS.gene73953:intron
GCAGCTGAAGCGGGTGAAAG+AGG 0.499828 5.2:-51000462 MS.gene73953:CDS
AGCATCAACAAAGAGATTAA+CGG 0.500852 5.2:+51000887 None:intergenic
CCGACCTTAAAAGCCAAGAA+TGG 0.501034 5.2:-51000635 MS.gene73953:CDS
AAAGACATATTATAAGCCTA+AGG 0.505882 5.2:-51000235 MS.gene73953:CDS
ATACAAACATAGTCAATCCT+TGG 0.507379 5.2:-50999496 MS.gene73953:CDS
AGATATTAGTTGGAATTCGC+AGG 0.508600 5.2:-51000500 MS.gene73953:CDS
TTTGAATACAGTTGAATTAG+CGG 0.512559 5.2:-51000415 MS.gene73953:CDS
ACTTGTTGCTTGGTTCATTG+AGG 0.515871 5.2:+50999329 None:intergenic
CTGAAGCGGGTGAAAGAGGC+CGG 0.516670 5.2:-51000458 MS.gene73953:CDS
AATCTTTGGAACCTTGGCAT+CGG 0.533073 5.2:+51001034 None:intergenic
CGCCAAACTGCTCCTCACTC+CGG 0.538251 5.2:-51000847 MS.gene73953:CDS
AAAATATGGCAGATATGTGC+AGG 0.539162 5.2:-51001059 MS.gene73953:CDS
GCCTGGCTCAGGCAGAGGCA+TGG 0.543238 5.2:+51001094 None:intergenic
TAACTTCCCTGCAGCTGAAG+CGG 0.546068 5.2:-51000472 MS.gene73953:CDS
TTTCATCAGGAAAAGTAGAA+TGG 0.549729 5.2:-50999420 MS.gene73953:CDS
ATGATGCCATGAAGATTGGT+TGG 0.554453 5.2:-51000311 MS.gene73953:CDS
ATGTGCAGGCCCCGATGCCA+AGG 0.559547 5.2:-51001045 MS.gene73953:intron
TTGCAAGCTTCAAGCTGCCA+AGG 0.562587 5.2:+50999479 None:intergenic
TTGTGTATTATCCGACAGCT+AGG 0.565398 5.2:-51000371 MS.gene73953:CDS
CCCGGCCAAGAAGACGAAGA+TGG 0.565421 5.2:-51000789 MS.gene73953:CDS
GAAATGATGCATGGAAAGGA+TGG 0.568677 5.2:+51000912 None:intergenic
TGATGCCATGAAGATTGGTT+GGG 0.569149 5.2:-51000310 MS.gene73953:CDS
TTTCACCCGCTTCAGCTGCA+GGG 0.570879 5.2:+51000466 None:intergenic
GGAACAACAGTAGGAGGAGG+AGG 0.574344 5.2:+51000810 None:intergenic
TCACACCAATTCCTAGCTGT+CGG 0.585707 5.2:+51000360 None:intergenic
TCTTGGCCGGGAACAACAGT+AGG 0.588215 5.2:+51000801 None:intergenic
CTTGGCCAGTTACCGGAGTG+AGG 0.591769 5.2:+51000835 None:intergenic
TGAATTAGCGGACAAGAACA+TGG 0.593074 5.2:-51000403 MS.gene73953:CDS
AAGACATATTATAAGCCTAA+GGG 0.594017 5.2:-51000234 MS.gene73953:CDS
TGTTGATGCTCTCACTGACG+AGG 0.594122 5.2:-51000874 MS.gene73953:CDS
TTAACGGCTGAAATGATGCA+TGG 0.595581 5.2:+51000903 None:intergenic
CCGGGAACAACAGTAGGAGG+AGG 0.601910 5.2:+51000807 None:intergenic
ACAGTAGACTACGTACTGGC+TGG 0.603214 5.2:-51000593 MS.gene73953:CDS
CCGGAATTTAACTGAGAGTG+CGG 0.606695 5.2:-51000439 MS.gene73953:CDS
TGGCCGGGAACAACAGTAGG+AGG 0.609021 5.2:+51000804 None:intergenic
AGTAGAATGGCAGGGCTGAG+TGG 0.615590 5.2:-50999407 MS.gene73953:CDS
ATCAAGTCAGCGAATTCATG+AGG 0.631551 5.2:+51000702 None:intergenic
GGCTGAAATGATGCATGGAA+AGG 0.633559 5.2:+51000908 None:intergenic
TCTTTGGAACCTTGGCATCG+GGG 0.654059 5.2:+51001036 None:intergenic
TGATGCATGGAAAGGATGGA+CGG 0.656958 5.2:+51000916 None:intergenic
TCAGGAAAAGTAGAATGGCA+GGG 0.663509 5.2:-50999415 MS.gene73953:CDS
TCTGACACAGAGCTATACAC+TGG 0.679314 5.2:-50999377 MS.gene73953:CDS
GACCTTAAAAGCCAAGAATG+GGG 0.680749 5.2:-51000633 MS.gene73953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAACAAATCATAAAATTA+TGG + chr5.2:51000346-51000365 None:intergenic 15.0%
!! AGTTAACCTATGATAAAAAA+TGG + chr5.2:51000895-51000914 None:intergenic 20.0%
!!! ATTTTTTATCATAGGTTAAC+TGG - chr5.2:51000894-51000913 MS.gene73953:CDS 20.0%
!!! TTCTTACCATTTTTTATCAT+AGG - chr5.2:51000886-51000905 MS.gene73953:CDS 20.0%
!!! TTTGTATTTTGCAAGATTTT+AGG + chr5.2:51000926-51000945 None:intergenic 20.0%
!!! TTTTTTATCATAGGTTAACT+GGG - chr5.2:51000895-51000914 MS.gene73953:CDS 20.0%
! AAAGACATATTATAAGCCTA+AGG - chr5.2:51000200-51000219 MS.gene73953:CDS 25.0%
! AAGACATATTATAAGCCTAA+GGG - chr5.2:51000201-51000220 MS.gene73953:CDS 25.0%
! TTTGAATACAGTTGAATTAG+CGG - chr5.2:51000020-51000039 MS.gene73953:intron 25.0%
!!! CTCAATTTTAGAATTGCATT+TGG + chr5.2:51000506-51000525 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5.2:50999418-50999437 None:intergenic 25.0%
AACAAGGGAATTAAGAATGT+TGG + chr5.2:51000555-51000574 None:intergenic 30.0%
ACATAGTAGATTCTTCTGTT+TGG - chr5.2:51000445-51000464 MS.gene73953:CDS 30.0%
AGCATCAACAAAGAGATTAA+CGG + chr5.2:50999551-50999570 None:intergenic 30.0%
ATACAAACATAGTCAATCCT+TGG - chr5.2:51000939-51000958 MS.gene73953:intron 30.0%
ATTCTTCAAATGTCCCTAAT+TGG - chr5.2:51000247-51000266 MS.gene73953:CDS 30.0%
GTAACGATCATAAAGAAACT+GGG + chr5.2:50999500-50999519 None:intergenic 30.0%
TACAATGAGAAATGCTTAGA+AGG + chr5.2:51000745-51000764 None:intergenic 30.0%
TTTCATCAGGAAAAGTAGAA+TGG - chr5.2:51001015-51001034 MS.gene73953:intron 30.0%
! CACATATCTGCCATATTTTA+GGG + chr5.2:50999375-50999394 None:intergenic 30.0%
! CATTTGGAACTTATCCTATA+CGG + chr5.2:51000490-51000509 None:intergenic 30.0%
! GTTTGATAATTGTGTTGAAG+TGG - chr5.2:51000360-51000379 MS.gene73953:CDS 30.0%
! TTGTCATTCAAAACTTTTGC+AGG + chr5.2:51000986-51001005 None:intergenic 30.0%
! TTTTGACATAGGAAACAAGA+TGG + chr5.2:50999675-50999694 None:intergenic 30.0%
!!! GCAAAAGTTTTGAATGACAA+GGG - chr5.2:51000986-51001005 MS.gene73953:intron 30.0%
!!! TGCAAAAGTTTTGAATGACA+AGG - chr5.2:51000985-51001004 MS.gene73953:intron 30.0%
AAAAACAGTAGACTACGTAC+TGG - chr5.2:50999838-50999857 MS.gene73953:intron 35.0%
AAAATATGGCAGATATGTGC+AGG - chr5.2:50999376-50999395 MS.gene73953:CDS 35.0%
AAAGTTTAATACTTCCCGCA+AGG - chr5.2:50999430-50999449 MS.gene73953:CDS 35.0%
AGATATTAGTTGGAATTCGC+AGG - chr5.2:50999935-50999954 MS.gene73953:intron 35.0%
CCTGAAAACAACAGAATCTT+TGG + chr5.2:50999899-50999918 None:intergenic 35.0%
GGAATTAAGAATGTTGGAGA+TGG + chr5.2:51000549-51000568 None:intergenic 35.0%
GGTAACGATCATAAAGAAAC+TGG + chr5.2:50999501-50999520 None:intergenic 35.0%
TCTATCAAGTGCAAGAACAA+GGG + chr5.2:51000570-51000589 None:intergenic 35.0%
TCTGCTGGAAAGATATTAGT+TGG - chr5.2:50999925-50999944 MS.gene73953:intron 35.0%
TTATCATAGGTTAACTGGGA+TGG - chr5.2:51000899-51000918 MS.gene73953:CDS 35.0%
! GAATTGGTGTGATTTTGTGA+TGG - chr5.2:51000086-51000105 MS.gene73953:intron 35.0%
! GCACATATCTGCCATATTTT+AGG + chr5.2:50999376-50999395 None:intergenic 35.0%
!! ATATCTGCCATATTTTAGGG+TGG + chr5.2:50999372-50999391 None:intergenic 35.0%
!! TAGTCTACTGTTTTTCACGT+AGG + chr5.2:50999833-50999852 None:intergenic 35.0%
!! TGTTGTGAGAGTTTTGACAT+AGG + chr5.2:50999686-50999705 None:intergenic 35.0%
!!! CCAAAGATTCTGTTGTTTTC+AGG - chr5.2:50999896-50999915 MS.gene73953:intron 35.0%
!!! TGATTTTGTGATGGATGCTA+AGG - chr5.2:51000095-51000114 MS.gene73953:intron 35.0%
AAACACTCCTGTTGCTAAGT+AGG - chr5.2:51000764-51000783 MS.gene73953:CDS 40.0%
ACAGTAGAGATTGTTCCCTT+AGG + chr5.2:51000219-51000238 None:intergenic 40.0%
AGATTCCCAACCAATCTTCA+TGG + chr5.2:51000133-51000152 None:intergenic 40.0%
ATACACTGGCAGTCCTAAAT+CGG - chr5.2:51001072-51001091 MS.gene73953:CDS 40.0%
ATCAAGTCAGCGAATTCATG+AGG + chr5.2:50999736-50999755 None:intergenic 40.0%
ATCAGGAAAAGTAGAATGGC+AGG - chr5.2:51001019-51001038 MS.gene73953:intron 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5.2:51000124-51000143 MS.gene73953:intron 40.0%
ATTATCCGACAGCTAGGAAT+TGG - chr5.2:51000070-51000089 MS.gene73953:intron 40.0%
CGACCTTAAAAGCCAAGAAT+GGG - chr5.2:50999801-50999820 MS.gene73953:intron 40.0%
GAAATGATGCATGGAAAGGA+TGG + chr5.2:50999526-50999545 None:intergenic 40.0%
GACCTTAAAAGCCAAGAATG+GGG - chr5.2:50999802-50999821 MS.gene73953:intron 40.0%
GATCTTTCCAAAGCTTGACT+TGG - chr5.2:50999753-50999772 MS.gene73953:intron 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5.2:51000120-51000139 MS.gene73953:intron 40.0%
GTCTATCAAGTGCAAGAACA+AGG + chr5.2:51000571-51000590 None:intergenic 40.0%
TCAAGATCCTACTTAGCAAC+AGG + chr5.2:51000774-51000793 None:intergenic 40.0%
TCAGGAAAAGTAGAATGGCA+GGG - chr5.2:51001020-51001039 MS.gene73953:intron 40.0%
TTAACGGCTGAAATGATGCA+TGG + chr5.2:50999535-50999554 None:intergenic 40.0%
TTGTGTATTATCCGACAGCT+AGG - chr5.2:51000064-51000083 MS.gene73953:intron 40.0%
! ATGAAGATTGGTTGGGAATC+TGG - chr5.2:51000132-51000151 MS.gene73953:intron 40.0%
! TGAATTAGCGGACAAGAACA+TGG - chr5.2:51000032-51000051 MS.gene73953:intron 40.0%
! TGATGCCATGAAGATTGGTT+GGG - chr5.2:51000125-51000144 MS.gene73953:intron 40.0%
!! AACCCCATTCTTGGCTTTTA+AGG + chr5.2:50999807-50999826 None:intergenic 40.0%
!! AATCTTTGGAACCTTGGCAT+CGG + chr5.2:50999404-50999423 None:intergenic 40.0%
!! AGAGTGAATCTTTGGAACCT+TGG + chr5.2:50999410-50999429 None:intergenic 40.0%
!! GTTTTCAGGAAAAACTCTGC+TGG - chr5.2:50999910-50999929 MS.gene73953:intron 40.0%
!! GTTTTGAATGACAAGGGTGA+TGG - chr5.2:51000992-51001011 MS.gene73953:intron 40.0%
!!! CCATTCTTGGCTTTTAAGGT+CGG + chr5.2:50999803-50999822 None:intergenic 40.0%
ACCTCAAGAATGCGCCAATT+AGG + chr5.2:51000264-51000283 None:intergenic 45.0%
ATACTTCCCGCAAGGTCATT+TGG - chr5.2:50999438-50999457 MS.gene73953:CDS 45.0%
CATGTTCCAAATGACCTTGC+GGG + chr5.2:50999447-50999466 None:intergenic 45.0%
CCGACCTTAAAAGCCAAGAA+TGG - chr5.2:50999800-50999819 MS.gene73953:intron 45.0%
CCGCACTCTCAGTTAAATTC+CGG + chr5.2:50999999-51000018 None:intergenic 45.0%
CCGGAATTTAACTGAGAGTG+CGG - chr5.2:50999996-51000015 MS.gene73953:intron 45.0%
CCTCAAGAATGCGCCAATTA+GGG + chr5.2:51000263-51000282 None:intergenic 45.0%
GACTTCTCCAAGTCAAGCTT+TGG + chr5.2:50999763-50999782 None:intergenic 45.0%
GCATGTTCCAAATGACCTTG+CGG + chr5.2:50999448-50999467 None:intergenic 45.0%
GGAGATTGTGACACCATTAG+GGG + chr5.2:50999479-50999498 None:intergenic 45.0%
GGCTGAAATGATGCATGGAA+AGG + chr5.2:50999530-50999549 None:intergenic 45.0%
GGGAGATTGTGACACCATTA+GGG + chr5.2:50999480-50999499 None:intergenic 45.0%
GGTTTCCATCTTCGTCTTCT+TGG + chr5.2:50999654-50999673 None:intergenic 45.0%
TCACACCAATTCCTAGCTGT+CGG + chr5.2:51000078-51000097 None:intergenic 45.0%
TCTGACACAGAGCTATACAC+TGG - chr5.2:51001058-51001077 MS.gene73953:CDS 45.0%
TGATGCATGGAAAGGATGGA+CGG + chr5.2:50999522-50999541 None:intergenic 45.0%
TGGGAGATTGTGACACCATT+AGG + chr5.2:50999481-50999500 None:intergenic 45.0%
! ACAAGGGTGATGGTTTCATC+AGG - chr5.2:51001002-51001021 MS.gene73953:intron 45.0%
!! AAGCTAGCTTTGTGCCGTAT+AGG - chr5.2:51000473-51000492 MS.gene73953:CDS 45.0%
!! ATCTTTGGAACCTTGGCATC+GGG + chr5.2:50999403-50999422 None:intergenic 45.0%
!!! GCCATATTTTAGGGTGGACA+CGG + chr5.2:50999366-50999385 None:intergenic 45.0%
!! TGAAATAAATATATAAAATT+TGG - chr5.2:51000420-51000439 MS.gene73953:CDS 5.0%
CATGCATGTCGTTCCCCTAA+TGG - chr5.2:50999463-50999482 MS.gene73953:CDS 50.0%
CCCTAATTGGCGCATTCTTG+AGG - chr5.2:51000260-51000279 MS.gene73953:CDS 50.0%
GCCGTGTCCACCCTAAAATA+TGG - chr5.2:50999362-50999381 MS.gene73953:CDS 50.0%
TAACTTCCCTGCAGCTGAAG+CGG - chr5.2:50999963-50999982 MS.gene73953:intron 50.0%
TCCATCTTCGTCTTCTTGGC+CGG + chr5.2:50999650-50999669 None:intergenic 50.0%
TGTTGATGCTCTCACTGACG+AGG - chr5.2:50999561-50999580 MS.gene73953:intron 50.0%
TTGCAAGCTTCAAGCTGCCA+AGG + chr5.2:51000959-51000978 None:intergenic 50.0%
! ACAGTAGACTACGTACTGGC+TGG - chr5.2:50999842-50999861 MS.gene73953:intron 50.0%
! GGCAACAAGTCAGCCGATTT+AGG + chr5.2:51001088-51001107 None:intergenic 50.0%
!! GTACTGGCTGGTCTCACTTT+CGG - chr5.2:50999854-50999873 MS.gene73953:intron 50.0%
!! TCTTTGGAACCTTGGCATCG+GGG + chr5.2:50999402-50999421 None:intergenic 50.0%
AACTTCCCTGCAGCTGAAGC+GGG - chr5.2:50999964-50999983 MS.gene73953:intron 55.0%
AGTAGAATGGCAGGGCTGAG+TGG - chr5.2:51001028-51001047 MS.gene73953:intron 55.0%
CAGTAGGAGGAGGAGGTTCT+TGG + chr5.2:50999621-50999640 None:intergenic 55.0%
CCATCTTCGTCTTCTTGGCC+GGG + chr5.2:50999649-50999668 None:intergenic 55.0%
CCTCCTCCTACTGTTGTTCC+CGG - chr5.2:50999628-50999647 MS.gene73953:intron 55.0%
CGTAGGTGCAACCCCATTCT+TGG + chr5.2:50999816-50999835 None:intergenic 55.0%
GGAACAACAGTAGGAGGAGG+AGG + chr5.2:50999628-50999647 None:intergenic 55.0%
GGAGGTTCTTGGCCAGTTAC+CGG + chr5.2:50999610-50999629 None:intergenic 55.0%
TACCGGAGTGAGGAGCAGTT+TGG + chr5.2:50999593-50999612 None:intergenic 55.0%
TCTTGGCCGGGAACAACAGT+AGG + chr5.2:50999637-50999656 None:intergenic 55.0%
TTTCACCCGCTTCAGCTGCA+GGG + chr5.2:50999972-50999991 None:intergenic 55.0%
!!! ATTTTAGGGTGGACACGGCC+TGG + chr5.2:50999361-50999380 None:intergenic 55.0%
CCCGGCCAAGAAGACGAAGA+TGG - chr5.2:50999646-50999665 MS.gene73953:intron 60.0%
CCGGGAACAACAGTAGGAGG+AGG + chr5.2:50999631-50999650 None:intergenic 60.0%
CGCCAAACTGCTCCTCACTC+CGG - chr5.2:50999588-50999607 MS.gene73953:intron 60.0%
CTGAAGCGGGTGAAAGAGGC+CGG - chr5.2:50999977-50999996 MS.gene73953:intron 60.0%
CTGCTCCTCACTCCGGTAAC+TGG - chr5.2:50999595-50999614 MS.gene73953:intron 60.0%
CTTGGCCAGTTACCGGAGTG+AGG + chr5.2:50999603-50999622 None:intergenic 60.0%
CTTTCACCCGCTTCAGCTGC+AGG + chr5.2:50999973-50999992 None:intergenic 60.0%
GCAGCTGAAGCGGGTGAAAG+AGG - chr5.2:50999973-50999992 MS.gene73953:intron 60.0%
TGGCCGGGAACAACAGTAGG+AGG + chr5.2:50999634-50999653 None:intergenic 60.0%
ATGTGCAGGCCCCGATGCCA+AGG - chr5.2:50999390-50999409 MS.gene73953:CDS 65.0%
ACACGGCCTGGCTCAGGCAG+AGG + chr5.2:50999349-50999368 None:intergenic 70.0%
GGGTGGACACGGCCTGGCTC+AGG + chr5.2:50999355-50999374 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 50999343 51001114 50999343 ID=MS.gene73953
chr5.2 mRNA 50999343 51001114 50999343 ID=MS.gene73953.t1;Parent=MS.gene73953
chr5.2 exon 51001046 51001114 51001046 ID=MS.gene73953.t1.exon1;Parent=MS.gene73953.t1
chr5.2 CDS 51001046 51001114 51001046 ID=cds.MS.gene73953.t1;Parent=MS.gene73953.t1
chr5.2 exon 51000176 51000955 51000176 ID=MS.gene73953.t1.exon2;Parent=MS.gene73953.t1
chr5.2 CDS 51000176 51000955 51000176 ID=cds.MS.gene73953.t1;Parent=MS.gene73953.t1
chr5.2 exon 50999343 50999549 50999343 ID=MS.gene73953.t1.exon3;Parent=MS.gene73953.t1
chr5.2 CDS 50999343 50999549 50999343 ID=cds.MS.gene73953.t1;Parent=MS.gene73953.t1
Gene Sequence

>MS.gene73953

ATGCCTCTGCCTGAGCCAGGCCGTGTCCACCCTAAAATATGGCAGATATGTGCAGGCCCCGATGCCAAGGTTCCAAAGATTCACTCTAAAGTTTAATACTTCCCGCAAGGTCATTTGGAACATGCATGTCGTTCCCCTAATGGTGTCACAATCTCCCAGTTTCTTTATGATCGTTACCGTCCATCCTTTCCATGCATCATTTCAGCCGTTAATCTCTTTGTTGATGCTCTCACTGACGAGGTTTTCGCCAAACTGCTCCTCACTCCGGTAACTGGCCAAGAACCTCCTCCTCCTACTGTTGTTCCCGGCCAAGAAGACGAAGATGGAAACCATCTTGTTTCCTATGTCAAAACTCTCACAACAACTGATACTAAATCTGTATTCAAGATACCTCATGAATTCGCTGACTTGATCTTTCCAAAGCTTGACTTGGAGAAGTCTCAAAACATCACTGTCACCGACCTTAAAAGCCAAGAATGGGGTTGCACCTACGTGAAAAACAGTAGACTACGTACTGGCTGGTCTCACTTTCGGAAAGAGAAGAAACTCGTAGCCAAAGATTCTGTTGTTTTCAGGAAAAACTCTGCTGGAAAGATATTAGTTGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAGCGGGTGAAAGAGGCCGGAATTTAACTGAGAGTGCGGTTTTGAATACAGTTGAATTAGCGGACAAGAACATGGAGTTTGAAGTTGTGTATTATCCGACAGCTAGGAATTGGTGTGATTTTGTGATGGATGCTAAGGTTGTAGATGATGCCATGAAGATTGGTTGGGAATCTGGAATGAGAGTCAAACTTACGTTGAAGAAAGATGAATCTTCAAATTCAAAGACATATTATAAGCCTAAGGGAACAATCTCTACTGTCTTTAATTATTCTTCAAATGTCCCTAATTGGCGCATTCTTGAGGTGCATATGCTTTCTTATTTTAATTATTGTTATCAAGTTTATAGTTTTATTAAAGTTGAATCCATAATTTTATGATTTGTTTGATAATTGTGTTGAAGTGGATACATGATTCATTTAGATGTTGAAACAATAATTAGATGAAATAAATATATAAAATTTGGAGACATAGTAGATTCTTCTGTTTGGAATGTAAGCTAGCTTTGTGCCGTATAGGATAAGTTCCAAATGCAATTCTAAAATTGAGACAGAAAATCATTAGAGTCTCCATCTCCAACATTCTTAATTCCCTTGTTCTTGCACTTGATAGACATCTCATGACACTTAATGTATACTTTATGTAATCAGATTACATTTTAGATACGATTGCTTGTTATTAATATAGACTTAATCGATTGTTAGTATATAACTTAACTTGCTTCAAAAGTGATATTCTTTAAGAAATAGTAATGAAGTTTAAGAACCTTCTAAGCATTTCTCATTGTAAACACTCCTGTTGCTAAGTAGGATCTTGATTTTTACTCATGTTATCAATTGATAAGAAACTTTTGATGTGTAAATACATATATAAACTAAGCTATCAAATATTTCAAATGATTGATATTGATTCTTACCATTTTTTATCATAGGTTAACTGGGATGGACCTAAAATCTTGCAAAATACAAACATAGTCAATCCTTGGCAGCTTGAAGCTTGCAACATACCTGCAAAAGTTTTGAATGACAAGGGTGATGGTTTCATCAGGAAAAGTAGAATGGCAGGGCTGAGTGGTACTTTGTCTGACACAGAGCTATACACTGGCAGTCCTAAATCGGCTGACTTGTTGCCTCAATGA
Protein sequence

>MS.gene73953.t1

MPLPEPGRVHPKIWQICAGPDAKFLYDRYRPSFPCIISAVNLFVDALTDEVFAKLLLTPVTGQEPPPPTVVPGQEDEDGNHLVSYVKTLTTTDTKSVFKIPHEFADLIFPKLDLEKSQNITVTDLKSQEWGCTYVKNSRLRTGWSHFRKEKKLVAKDSVVFRKNSAGKILVGIRRNTNFPAAEAGERGRNLTESAVLNTVELADKNMEFEVVYYPTARNWCDFVMDAKVVDDAMKIGWESGMRVKLTLKKDESSNSKTYYKPKGTISTVFNYSSNVPNWRILEVNWDGPKILQNTNIVNPWQLEACNIPAKVLNDKGDGFIRKSRMAGLSGTLSDTELYTGSPKSADLLPQ