AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73969

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73969.t1 MTR_5g061220 92.424 528 39 1 1 527 1 528 0.0 1004
MS.gene73969.t1 MTR_1g058210 53.448 522 166 8 8 525 1 449 6.66e-172 493
MS.gene73969.t1 MTR_5g040880 50.000 546 206 11 14 527 6 516 5.36e-159 463
MS.gene73969.t1 MTR_2g024430 49.174 545 215 14 6 525 7 514 2.70e-157 458
MS.gene73969.t1 MTR_5g082140 48.560 521 187 11 14 521 7 459 3.67e-150 438
MS.gene73969.t1 MTR_3g073420 44.704 557 254 15 13 526 13 558 2.53e-142 423
MS.gene73969.t1 MTR_5g060630 46.875 544 191 13 8 479 1 518 1.52e-140 416
MS.gene73969.t1 MTR_5g460740 46.875 544 191 13 8 479 1 518 1.52e-140 416
MS.gene73969.t1 MTR_2g006270 46.512 516 192 13 16 523 10 449 7.23e-132 391
MS.gene73969.t1 MTR_5g060780 52.792 394 141 10 8 391 1 359 9.75e-128 379
MS.gene73969.t1 MTR_5g061890 55.313 367 126 8 8 367 1 336 8.25e-127 376
MS.gene73969.t1 MTR_5g460920 52.792 394 141 10 8 391 1 359 2.24e-126 375
MS.gene73969.t1 MTR_2g006380 42.802 521 199 16 15 523 16 449 4.17e-115 348
MS.gene73969.t1 MTR_5g040740 42.095 525 165 12 15 526 8 406 7.95e-113 341
MS.gene73969.t1 MTR_2g094570 39.448 507 240 15 19 477 11 498 1.62e-99 315
MS.gene73969.t1 MTR_2g094570 39.448 507 240 15 19 477 11 498 1.75e-99 315
MS.gene73969.t1 MTR_1g064430 37.478 563 282 14 14 523 22 567 6.15e-99 314
MS.gene73969.t1 MTR_1g064430 37.478 563 282 14 14 523 35 580 9.03e-99 313
MS.gene73969.t1 MTR_2g094570 39.448 507 240 15 19 477 67 554 1.06e-98 315
MS.gene73969.t1 MTR_1g094960 40.476 462 225 14 7 431 7 455 1.61e-94 300
MS.gene73969.t1 MTR_7g101280 37.684 475 223 12 14 437 26 478 1.67e-94 301
MS.gene73969.t1 MTR_0041s0110 37.684 475 223 12 14 437 26 478 1.67e-94 301
MS.gene73969.t1 MTR_4g058930 40.355 451 217 11 19 431 12 448 1.38e-92 296
MS.gene73969.t1 MTR_4g058930 40.355 451 217 11 19 431 12 448 1.83e-92 297
MS.gene73969.t1 MTR_5g062970 60.101 198 71 3 16 208 35 229 5.90e-78 245
MS.gene73969.t1 MTR_5g062960 62.312 199 47 4 263 455 1 177 5.46e-72 228
MS.gene73969.t1 MTR_7g101275 39.939 328 157 8 29 345 2 300 5.42e-69 227
MS.gene73969.t1 MTR_5g060770 41.253 383 133 11 70 437 9 314 6.29e-68 223
MS.gene73969.t1 MTR_0041s0120 38.739 333 176 8 29 350 2 317 9.05e-68 224
MS.gene73969.t1 MTR_2g014770 33.763 388 197 10 1 366 38 387 6.92e-66 224
MS.gene73969.t1 MTR_2g014770 33.763 388 197 10 1 366 38 387 1.61e-65 225
MS.gene73969.t1 MTR_2g018690 35.840 399 208 11 6 384 6 376 1.75e-61 217
MS.gene73969.t1 MTR_2g005240 34.606 393 214 8 19 394 23 389 3.05e-60 211
MS.gene73969.t1 MTR_4g124900 35.150 367 199 7 14 368 18 357 5.08e-60 214
MS.gene73969.t1 MTR_4g124900 35.150 367 199 7 14 368 18 357 6.37e-60 214
MS.gene73969.t1 MTR_4g021580 34.375 384 203 9 19 391 19 364 1.29e-59 208
MS.gene73969.t1 MTR_4g021580 34.375 384 203 9 19 391 19 364 1.98e-59 208
MS.gene73969.t1 MTR_4g088210 35.040 371 186 10 19 366 38 376 2.38e-59 209
MS.gene73969.t1 MTR_2g043250 34.060 367 203 6 14 368 18 357 3.17e-59 212
MS.gene73969.t1 MTR_4g088210 35.040 371 186 10 19 366 38 376 4.13e-59 208
MS.gene73969.t1 MTR_8g100050 33.943 383 207 9 19 385 59 411 4.71e-59 208
MS.gene73969.t1 MTR_8g100050 33.943 383 207 9 19 385 59 411 1.30e-58 208
MS.gene73969.t1 MTR_8g079492 35.013 397 213 11 6 384 6 375 5.84e-58 207
MS.gene73969.t1 MTR_8g079492 35.013 397 213 11 6 384 6 375 6.54e-58 207
MS.gene73969.t1 MTR_7g062540 31.403 449 261 13 19 448 14 434 2.29e-57 203
MS.gene73969.t1 MTR_5g076270 34.286 385 209 10 16 384 21 377 2.80e-57 205
MS.gene73969.t1 MTR_2g093740 33.248 391 209 11 19 388 56 415 2.96e-57 203
MS.gene73969.t1 MTR_3g064050 33.333 384 214 8 16 384 23 379 6.87e-57 204
MS.gene73969.t1 MTR_2g093740 33.248 391 209 11 19 388 56 415 8.60e-57 202
MS.gene73969.t1 MTR_2g093740 33.248 391 209 11 19 388 56 415 1.21e-56 202
MS.gene73969.t1 MTR_1g024025 34.426 366 200 8 14 366 38 376 2.62e-56 204
MS.gene73969.t1 MTR_8g027440 32.019 431 227 11 19 438 29 404 3.96e-55 197
MS.gene73969.t1 MTR_8g101360 33.967 368 202 9 16 369 28 368 1.13e-54 199
MS.gene73969.t1 MTR_2g093740 32.178 404 209 12 19 388 56 428 1.11e-53 193
MS.gene73969.t1 MTR_2g093740 32.178 404 209 12 19 388 56 428 2.92e-53 192
MS.gene73969.t1 MTR_2g093740 32.178 404 209 12 19 388 56 428 4.39e-53 193
MS.gene73969.t1 MTR_4g060460 34.015 391 206 11 19 385 54 416 1.47e-51 188
MS.gene73969.t1 MTR_4g060460 34.015 391 206 11 19 385 54 416 1.59e-51 188
MS.gene73969.t1 MTR_5g460910 36.520 408 138 15 51 437 2 309 2.49e-49 173
MS.gene73969.t1 MTR_8g079492 35.535 318 166 9 79 384 3 293 4.27e-45 170
MS.gene73969.t1 MTR_2g093740 33.223 301 156 8 102 388 16 285 3.44e-41 157
MS.gene73969.t1 MTR_2g093740 33.223 301 156 8 102 388 16 285 4.80e-41 157
MS.gene73969.t1 MTR_2g093740 31.847 314 156 9 102 388 16 298 6.14e-38 147
MS.gene73969.t1 MTR_2g093740 31.847 314 156 9 102 388 16 298 1.07e-37 147
MS.gene73969.t1 MTR_8g446900 28.000 300 188 5 70 366 14 288 2.73e-32 128
MS.gene73969.t1 MTR_5g046120 39.130 115 57 4 57 163 12 121 2.13e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73969.t1 AT1G77850 38.368 576 286 14 1 526 1 557 9.48e-118 359
MS.gene73969.t1 AT1G77850 38.368 576 286 14 1 526 1 557 1.42e-117 359
MS.gene73969.t1 AT4G30080 38.267 554 279 19 19 526 19 555 3.35e-94 301
MS.gene73969.t1 AT2G28350 42.327 404 190 11 19 388 10 404 1.11e-86 281
MS.gene73969.t1 AT2G28350 42.327 404 190 11 19 388 10 404 1.13e-86 281
MS.gene73969.t1 AT2G46530 36.170 376 206 8 18 385 20 369 1.70e-65 223
MS.gene73969.t1 AT2G46530 36.170 376 206 8 18 385 41 390 3.41e-65 223
MS.gene73969.t1 AT3G61830 34.933 375 200 9 15 381 21 359 1.47e-62 215
MS.gene73969.t1 AT2G33860 35.150 367 187 8 19 366 54 388 1.95e-62 215
MS.gene73969.t1 AT1G30330 35.659 387 205 9 14 384 18 376 1.04e-61 218
MS.gene73969.t1 AT1G30330 35.659 387 205 9 14 384 20 378 1.05e-61 218
MS.gene73969.t1 AT1G30330 35.659 387 205 9 14 384 20 378 1.05e-61 218
MS.gene73969.t1 AT4G23980 30.309 485 283 14 19 483 12 461 2.65e-59 207
MS.gene73969.t1 AT5G37020 33.835 399 221 10 16 399 19 389 5.30e-59 209
MS.gene73969.t1 AT5G37020 33.835 399 221 10 16 399 19 389 1.21e-58 208
MS.gene73969.t1 AT1G59750 34.247 365 204 6 19 373 22 360 3.23e-58 205
MS.gene73969.t1 AT1G59750 34.247 365 204 6 19 373 22 360 3.23e-58 205
MS.gene73969.t1 AT1G59750 34.247 365 204 6 19 373 22 360 3.48e-58 205
MS.gene73969.t1 AT1G59750 34.247 365 204 6 19 373 22 360 3.59e-58 205
MS.gene73969.t1 AT5G60450 34.783 391 201 13 13 382 35 392 5.22e-58 206
MS.gene73969.t1 AT5G60450 34.794 388 199 13 16 382 62 416 1.24e-57 205
MS.gene73969.t1 AT4G23980 30.801 487 280 16 19 483 12 463 1.24e-57 203
MS.gene73969.t1 AT5G20730 34.146 369 200 7 14 368 19 358 2.02e-57 207
MS.gene73969.t1 AT1G19220 33.333 387 214 9 14 385 18 375 6.90e-57 205
MS.gene73969.t1 AT5G62000 32.915 398 223 10 3 385 45 413 3.35e-56 202
MS.gene73969.t1 AT5G62000 32.915 398 223 10 3 385 45 413 3.76e-56 202
MS.gene73969.t1 AT5G62000 32.915 398 223 10 3 385 45 413 3.76e-56 202
MS.gene73969.t1 AT5G62000 32.915 398 223 10 3 385 45 413 3.76e-56 202
MS.gene73969.t1 AT5G62000 32.915 398 223 10 3 385 45 413 3.76e-56 202
MS.gene73969.t1 AT5G20730 34.054 370 200 8 14 368 19 359 8.15e-56 202
MS.gene73969.t1 AT5G20730 34.054 370 200 8 14 368 19 359 1.05e-55 202
MS.gene73969.t1 AT1G19850 33.007 409 229 12 16 408 51 430 1.59e-54 197
MS.gene73969.t1 AT1G19850 33.007 409 229 12 16 408 40 419 1.84e-54 197
MS.gene73969.t1 AT1G34390 32.199 382 212 10 19 392 27 369 9.28e-54 191
MS.gene73969.t1 AT1G34410 31.139 395 232 9 19 405 27 389 2.85e-53 190
MS.gene73969.t1 AT1G35520 31.646 395 230 9 19 405 27 389 7.20e-53 189
MS.gene73969.t1 AT1G34310 31.407 398 233 8 16 405 24 389 7.50e-53 189
MS.gene73969.t1 AT1G35540 28.238 471 295 11 19 480 27 463 4.65e-51 184
MS.gene73969.t1 AT1G35240 31.552 393 219 11 19 398 27 382 1.56e-50 182
MS.gene73969.t1 AT3G61830 34.639 332 177 8 54 381 13 308 3.19e-49 178
MS.gene73969.t1 AT1G34170 29.243 489 279 16 19 486 25 467 6.36e-47 171
MS.gene73969.t1 AT2G33860 34.630 257 134 4 117 366 2 231 8.57e-46 167
MS.gene73969.t1 AT2G46530 37.354 257 136 4 114 369 34 266 2.88e-45 167
MS.gene73969.t1 AT1G34170 27.485 513 276 17 19 507 25 465 1.47e-41 157
MS.gene73969.t1 AT5G37020 34.932 292 153 8 117 399 31 294 1.63e-41 159
MS.gene73969.t1 AT1G34170 28.016 489 259 16 19 486 25 441 1.15e-40 153
MS.gene73969.t1 AT1G43950 37.745 204 113 5 19 216 27 222 2.12e-36 135

Find 150 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5.2:-51335560 MS.gene73969:CDS
TTCAAACTACTGAGGTTATT+TGG 0.203805 5.2:+51335638 None:intergenic
GGCACATGTCTGCCAGAATT+TGG 0.214791 5.2:+51338770 None:intergenic
TTTCTTTATCATAGGTTAAC+TGG 0.218017 5.2:-51336015 MS.gene73969:intron
GTTTGGTTCAAAGATCTCTT+AGG 0.241864 5.2:+51335815 None:intergenic
ACGGCGATTGTTTGAGCATT+AGG 0.246879 5.2:+51338666 None:intergenic
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5.2:-51337809 MS.gene73969:intron
CATGATCATTTATCTCTATC+AGG 0.276134 5.2:-51335746 MS.gene73969:CDS
CGGCGATTGTTTGAGCATTA+GGG 0.276924 5.2:+51338667 None:intergenic
GGCAGAATCCTGAATTAGTT+TGG 0.277148 5.2:+51335886 None:intergenic
CTCAGTAGTTTGAACACCTT+TGG 0.299252 5.2:-51335629 MS.gene73969:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5.2:-51337990 MS.gene73969:CDS
TACCGGACTGAGTAGCAATT+TGG 0.306452 5.2:+51338560 None:intergenic
GTCAATGCATCAAATTTCAT+TGG 0.331745 5.2:+51335466 None:intergenic
AACCACAATTCAACAATGTT+TGG 0.332975 5.2:+51335798 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5.2:-51338344 MS.gene73969:CDS
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5.2:-51337928 MS.gene73969:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5.2:+51337808 None:intergenic
ATTCAGGATTCTGCCTTGTT+TGG 0.357536 5.2:-51335878 MS.gene73969:CDS
AATTTCATTGGATCCCTTAG+AGG 0.360856 5.2:+51335478 None:intergenic
GGAGAATTTAAGTCAAGCTT+AGG 0.361917 5.2:+51338393 None:intergenic
CAAACAATCGCCGTTATTGA+TGG 0.364171 5.2:-51338657 MS.gene73969:CDS
AGTTCTTGTCCTTCATTTAC+CGG 0.366655 5.2:+51338543 None:intergenic
GAAAGTGATGTCCATGATTC+TGG 0.375162 5.2:-51335518 MS.gene73969:CDS
GAGTCCAACCCGTAGTAAAA+AGG 0.376026 5.2:+51338283 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5.2:+51337809 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5.2:+51338729 None:intergenic
TGCCTCAATAACCGCCTTCT+CGG 0.388165 5.2:+51338050 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5.2:-51335713 MS.gene73969:CDS
GCCGCGTGAAATCCAAATTC+TGG 0.394351 5.2:-51338782 MS.gene73969:CDS
GCAGAATCCTGAATTAGTTT+GGG 0.395084 5.2:+51335887 None:intergenic
CAGCAGGCATGCAGGGAGCC+AGG 0.396063 5.2:-51335769 MS.gene73969:CDS
GGGACAGACATGGTGGCCGG+TGG 0.403787 5.2:+51338813 None:intergenic
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5.2:-51337822 MS.gene73969:CDS
TAGATGCACTGTAGGATTGC+GGG 0.423120 5.2:+51335931 None:intergenic
TGGTTTGGAGGTCAAGGGAG+AGG 0.427341 5.2:-51338106 MS.gene73969:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5.2:+51335718 None:intergenic
TTTGGTTCAAAGATCTCTTA+GGG 0.434535 5.2:+51335816 None:intergenic
TCAGTAGTTTGAACACCTTT+GGG 0.434981 5.2:-51335628 MS.gene73969:CDS
ATCAAAACCGCGTCAATCCT+TGG 0.437926 5.2:-51335970 MS.gene73969:CDS
CAAGGGAGAAGATTCCTCTA+AGG 0.441291 5.2:-51335492 MS.gene73969:CDS
AGAGAATGGTTTGGAGGTCA+AGG 0.444816 5.2:-51338112 MS.gene73969:CDS
TTGGATTTCACGCGGCGTGC+CGG 0.446354 5.2:+51338789 None:intergenic
TTGAAGTTATATATTACCCT+AGG 0.447607 5.2:-51337999 MS.gene73969:CDS
TCTTCTTCATCAACAACCTC+AGG 0.455552 5.2:+51338519 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5.2:-51338562 MS.gene73969:CDS
AAGGAGGAGGGTAAAGAGAA+TGG 0.463485 5.2:-51338126 MS.gene73969:CDS
GAACTCTATCATAGGAATGA+AGG 0.464627 5.2:+51335838 None:intergenic
AACACAAAAGAAGTTGGTTC+TGG 0.471305 5.2:-51335578 MS.gene73969:CDS
CTCTTAGGGAACTCTATCAT+AGG 0.472447 5.2:+51335830 None:intergenic
GGAGGGTAAAGAGAATGGTT+TGG 0.480150 5.2:-51338121 MS.gene73969:CDS
TGAAGAAGAAGACGGTGATA+AGG 0.483386 5.2:-51338505 MS.gene73969:CDS
GTGGATGATGCTATGAAGAT+TGG 0.483610 5.2:-51337940 MS.gene73969:CDS
ACTGCTGGAGATATAGTTGT+TGG 0.484150 5.2:-51338204 MS.gene73969:CDS
ACAAGATCTGATTCTAATAA+CGG 0.488893 5.2:-51338459 MS.gene73969:CDS
TTCTTTATCATAGGTTAACT+GGG 0.489462 5.2:-51336014 MS.gene73969:intron
GGTTTGGAGGTCAAGGGAGA+GGG 0.491439 5.2:-51338105 MS.gene73969:CDS
GGTTATTGAGGCAGTGGAGT+TGG 0.491818 5.2:-51338040 MS.gene73969:CDS
TGGAACATTCACCGATGGAC+CGG 0.493269 5.2:+51338749 None:intergenic
GAGAATTTAAGTCAAGCTTA+GGG 0.494979 5.2:+51338394 None:intergenic
TTACTTCCCACATGGTCACT+TGG 0.499922 5.2:-51338706 MS.gene73969:CDS
GGCCACCATGTCTGTCCCTC+CGG 0.501925 5.2:-51338808 MS.gene73969:CDS
AGTGAATAATAAGAAGGAGG+AGG 0.508094 5.2:-51338139 MS.gene73969:CDS
GATTTCACGCGGCGTGCCGG+AGG 0.509842 5.2:+51338792 None:intergenic
CTCCCTTGCTACCAGAATCA+TGG 0.511654 5.2:+51335507 None:intergenic
TTCCGCCATGTCTTCCCCAC+CGG 0.514610 5.2:-51338829 None:intergenic
AAATTCTGGCAGACATGTGC+CGG 0.515291 5.2:-51338768 MS.gene73969:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5.2:+51338814 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5.2:-51338760 MS.gene73969:CDS
GGTGGCCGGTGGGGAAGACA+TGG 0.519543 5.2:+51338824 None:intergenic
AAAGGCAGTGAATAATAAGA+AGG 0.519919 5.2:-51338145 MS.gene73969:CDS
GAAGACTCATCAATCTTCAA+AGG 0.523956 5.2:+51337889 None:intergenic
GAGAGTTCCTGAGACGGAAA+AGG 0.524822 5.2:+51338372 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5.2:+51338815 None:intergenic
GGAGTTGGCGGAAAAGAATA+CGG 0.527090 5.2:-51338025 MS.gene73969:CDS
AACCGAGAAGGCGGTTATTG+AGG 0.529145 5.2:-51338052 MS.gene73969:CDS
CTACTCAGTCCGGTAAATGA+AGG 0.531469 5.2:-51338552 MS.gene73969:CDS
GACCTCATCGGTGTGAGGAT+CGG 0.532501 5.2:+51338587 None:intergenic
GAGAATGGTTTGGAGGTCAA+GGG 0.534684 5.2:-51338111 MS.gene73969:CDS
AACAGCAACACAAAAGAAGT+TGG 0.534960 5.2:-51335584 MS.gene73969:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5.2:+51338810 None:intergenic
AACTCTATCATAGGAATGAA+GGG 0.541293 5.2:+51335839 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.546005 5.2:-51338118 MS.gene73969:CDS
AAGGGAGAAGATTCCTCTAA+GGG 0.546926 5.2:-51335491 MS.gene73969:CDS
ATTGTTTGAGCATTAGGGGA+AGG 0.547154 5.2:+51338672 None:intergenic
TCTGATTCTAATAACGGCGG+TGG 0.547379 5.2:-51338453 MS.gene73969:CDS
TTGAGCATTAGGGGAAGGGC+AGG 0.547426 5.2:+51338677 None:intergenic
GTGAATAATAAGAAGGAGGA+GGG 0.548645 5.2:-51338138 MS.gene73969:CDS
TATTGAGGCAGTGGAGTTGG+CGG 0.551866 5.2:-51338037 MS.gene73969:CDS
AACCAAACATTGTTGAATTG+TGG 0.552042 5.2:-51335800 MS.gene73969:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.552664 5.2:+51335953 None:intergenic
TAGAGATAAATGATCATGCC+TGG 0.553837 5.2:+51335751 None:intergenic
TTGTTTGAGCATTAGGGGAA+GGG 0.555788 5.2:+51338673 None:intergenic
GTCCATGATTCTGGTAGCAA+GGG 0.557565 5.2:-51335509 MS.gene73969:CDS
GAAGGCGGTTATTGAGGCAG+TGG 0.558765 5.2:-51338046 MS.gene73969:CDS
ACCTTTGGAACATTCACCGA+TGG 0.560091 5.2:+51338744 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 5.2:-51337936 MS.gene73969:CDS
TGAAGTTATATATTACCCTA+GGG 0.565837 5.2:-51337998 MS.gene73969:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5.2:+51335658 None:intergenic
ATTTCACGCGGCGTGCCGGA+GGG 0.569870 5.2:+51338793 None:intergenic
TGCCTTGTTTGGTGATAACA+AGG 0.570662 5.2:-51335867 MS.gene73969:CDS
AAATTGCACCAATTAGCCCT+AGG 0.571867 5.2:+51337982 None:intergenic
TGTAGTTGATGCAAATGTTG+TGG 0.574224 5.2:-51337959 MS.gene73969:CDS
GTTTGCTGCAGCTGAAGCAA+AGG 0.577997 5.2:-51338163 MS.gene73969:CDS
CAGCAAACTTCATATTTCTG+CGG 0.579993 5.2:+51338178 None:intergenic
AGGAATGGTAGATGCACTGT+AGG 0.580449 5.2:+51335923 None:intergenic
TGATGCTATGAAGATTGGTT+GGG 0.580467 5.2:-51337935 MS.gene73969:CDS
TGTCCATGATTCTGGTAGCA+AGG 0.582395 5.2:-51335510 MS.gene73969:CDS
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5.2:+51335659 None:intergenic
CATGCGCCAATTAGGGACAC+TGG 0.600814 5.2:+51337816 None:intergenic
GTGATGATACAGCGAAAGGA+AGG 0.607040 5.2:+51338627 None:intergenic
GGCGATTGTTTGAGCATTAG+GGG 0.607178 5.2:+51338668 None:intergenic
CTCAACAAAATCTATCCCAA+AGG 0.608187 5.2:+51335613 None:intergenic
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5.2:-51338321 MS.gene73969:CDS
GTAGATGCACTGTAGGATTG+CGG 0.614662 5.2:+51335930 None:intergenic
ACCCTTGTTATCACCAAACA+AGG 0.615356 5.2:+51335865 None:intergenic
GGCCGTGATGATACAGCGAA+AGG 0.619072 5.2:+51338623 None:intergenic
GCATGTTCCAAGTGACCATG+TGG 0.620759 5.2:+51338699 None:intergenic
TCCATCGGTGAATGTTCCAA+AGG 0.620764 5.2:-51338745 MS.gene73969:CDS
TTCCTTTCGCTGTATCATCA+CGG 0.622538 5.2:-51338625 MS.gene73969:CDS
AGGACGGTAACCATCAATAA+CGG 0.623715 5.2:+51338647 None:intergenic
GGCAGTGAATAATAAGAAGG+AGG 0.624122 5.2:-51338142 MS.gene73969:CDS
CAACTTGCCAAGGATTGACG+CGG 0.626664 5.2:+51335963 None:intergenic
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5.2:+51338575 None:intergenic
AAGGGGAATACTAACCGAGA+AGG 0.636629 5.2:-51338064 MS.gene73969:CDS
AATTGCACCAATTAGCCCTA+GGG 0.639117 5.2:+51337983 None:intergenic
GCGTGCCGGAGGGACAGACA+TGG 0.644657 5.2:+51338803 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 5.2:+51338420 None:intergenic
TCCGTCATGAACATCAGTAA+CGG 0.649838 5.2:+51338350 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5.2:+51338582 None:intergenic
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5.2:-51338320 MS.gene73969:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5.2:-51338351 MS.gene73969:CDS
AAAGTTTATTACTTCCCACA+TGG 0.655323 5.2:-51338714 MS.gene73969:CDS
CATCGGTGTGAGGATCGGCT+AGG 0.655915 5.2:+51338592 None:intergenic
AGCCGATCCTCACACCGATG+AGG 0.659256 5.2:-51338589 MS.gene73969:CDS
GCCAGAATTTGGATTTCACG+CGG 0.659715 5.2:+51338781 None:intergenic
GGGAATACTAACCGAGAAGG+CGG 0.664523 5.2:-51338061 MS.gene73969:CDS
TGATACAGCGAAAGGAAGGA+CGG 0.665109 5.2:+51338631 None:intergenic
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5.2:+51337841 None:intergenic
CAAAGGTGTTCAAACTACTG+AGG 0.677729 5.2:+51335630 None:intergenic
TGCCGGAGGGACAGACATGG+TGG 0.679386 5.2:+51338806 None:intergenic
GTTGTTGATGAAGAAGAAGA+CGG 0.680880 5.2:-51338513 MS.gene73969:CDS
TGAAGGACAAGAACTTCCTG+AGG 0.682154 5.2:-51338535 MS.gene73969:CDS
GTAACGGAGAGTTCCTGAGA+CGG 0.687969 5.2:+51338366 None:intergenic
AGGATCGGCTAGGAGATCAA+CGG 0.692255 5.2:+51338602 None:intergenic
TCTTGTAAGAGTCTTTACGA+AGG 0.695672 5.2:+51338476 None:intergenic
CATGTTCCAAGTGACCATGT+GGG 0.696959 5.2:+51338700 None:intergenic
GGAAGATCAAGTCAGCACAA+AGG 0.704708 5.2:+51338415 None:intergenic
GCCTTGTTTGGTGATAACAA+GGG 0.716105 5.2:-51335866 MS.gene73969:CDS
AGATCTGATTCTAATAACGG+CGG 0.725286 5.2:-51338456 MS.gene73969:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAGTATACAAATATTA+GGG - chr5.2:51337667-51337686 MS.gene73969:intron 10.0%
!!! ATGCATTTTATTATAAAATT+AGG + chr5.2:51338130-51338149 None:intergenic 10.0%
!!! TATTTTATTTTTTGAATCAA+GGG - chr5.2:51336541-51336560 MS.gene73969:intron 10.0%
!!! TGCATTTTATTATAAAAATA+TGG + chr5.2:51338095-51338114 None:intergenic 10.0%
!!! TTATTTTATTTTTTGAATCA+AGG - chr5.2:51336540-51336559 MS.gene73969:intron 10.0%
!! ATAGTTTAGTATACTTAATT+AGG + chr5.2:51336752-51336771 None:intergenic 15.0%
!! CAATTTAGTATACAAATATT+AGG - chr5.2:51337666-51337685 MS.gene73969:intron 15.0%
!! TAATATTACAAACAATTTGA+AGG - chr5.2:51336774-51336793 MS.gene73969:intron 15.0%
!!! AATTACTTGTATTAGAATTT+TGG + chr5.2:51337300-51337319 None:intergenic 15.0%
!!! GATTTTTATTTCTTTATCAT+AGG - chr5.2:51338274-51338293 MS.gene73969:CDS 15.0%
!!! TATATTTTGAATAACTGTAT+TGG - chr5.2:51338243-51338262 MS.gene73969:CDS 15.0%
!! ACATTGATACAAAAGTAAAA+AGG + chr5.2:51336872-51336891 None:intergenic 20.0%
!! ACCAAAAAACAGTAAATTAA+CGG + chr5.2:51337742-51337761 None:intergenic 20.0%
!! CCAAAATTAATCCATAATTA+TGG - chr5.2:51337873-51337892 MS.gene73969:CDS 20.0%
!! TCTTTGAATGATAATAGTTA+TGG - chr5.2:51338584-51338603 MS.gene73969:CDS 20.0%
!! TTGTATACTAAATTGATCAA+GGG + chr5.2:51337661-51337680 None:intergenic 20.0%
!! TTTGTATACTAAATTGATCA+AGG + chr5.2:51337662-51337681 None:intergenic 20.0%
!!! AGTGTAAAAAAAAGTTTTTC+GGG + chr5.2:51336836-51336855 None:intergenic 20.0%
!!! CCATAATTATGGATTAATTT+TGG + chr5.2:51337876-51337895 None:intergenic 20.0%
!!! GAGTGTAAAAAAAAGTTTTT+CGG + chr5.2:51336837-51336856 None:intergenic 20.0%
!!! TGGAAAAGTATTAGTTATTT+AGG - chr5.2:51338066-51338085 MS.gene73969:CDS 20.0%
! AAATATTAGGGTTAGGTTAT+AGG - chr5.2:51337679-51337698 MS.gene73969:intron 25.0%
! ACAAGATCTGATTCTAATAA+CGG - chr5.2:51335838-51335857 MS.gene73969:CDS 25.0%
! ACTATTATCATTCAAAGAGT+TGG + chr5.2:51338582-51338601 None:intergenic 25.0%
! AGTATACAAATATTAGGGTT+AGG - chr5.2:51337672-51337691 MS.gene73969:intron 25.0%
! ATGATTACCAAAATACAGTT+AGG - chr5.2:51337411-51337430 MS.gene73969:intron 25.0%
! CCAAAAAACAGTAAATTAAC+GGG + chr5.2:51337741-51337760 None:intergenic 25.0%
! CCTTCAACACTAATATAAAA+AGG - chr5.2:51337776-51337795 MS.gene73969:intron 25.0%
! TATTATTGTCGAAGAAGTTT+AGG - chr5.2:51336595-51336614 MS.gene73969:intron 25.0%
! TGAAGTTATATATTACCCTA+GGG - chr5.2:51336299-51336318 MS.gene73969:intron 25.0%
! TTCTTTATCATAGGTTAACT+GGG - chr5.2:51338283-51338302 MS.gene73969:CDS 25.0%
! TTGAAGTTATATATTACCCT+AGG - chr5.2:51336298-51336317 MS.gene73969:intron 25.0%
! TTTCTTTATCATAGGTTAAC+TGG - chr5.2:51338282-51338301 MS.gene73969:CDS 25.0%
!! ATTTGCTCATTTTCTTAAGT+AGG - chr5.2:51338148-51338167 MS.gene73969:CDS 25.0%
!! GATTTTTCCTAACTGTATTT+TGG + chr5.2:51337421-51337440 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG - chr5.2:51338737-51338756 MS.gene73969:CDS 25.0%
!!! AGTTTTTTAGTGTTCACAAA+CGG + chr5.2:51336039-51336058 None:intergenic 25.0%
!!! ATAAATAATGGATAAGCTTC+AGG - chr5.2:51338036-51338055 MS.gene73969:CDS 25.0%
!!! CCTTTTTATATTAGTGTTGA+AGG + chr5.2:51337779-51337798 None:intergenic 25.0%
!!! GTTTTCTGGCATATAAATAA+TGG - chr5.2:51338024-51338043 MS.gene73969:CDS 25.0%
AAAAAAGCCCAAACTAATTC+AGG - chr5.2:51338403-51338422 MS.gene73969:CDS 30.0%
AAAGGCAGTGAATAATAAGA+AGG - chr5.2:51336152-51336171 MS.gene73969:intron 30.0%
AAAGTTTATTACTTCCCACA+TGG - chr5.2:51335583-51335602 MS.gene73969:CDS 30.0%
AACCAAACATTGTTGAATTG+TGG - chr5.2:51338497-51338516 MS.gene73969:CDS 30.0%
AACCACAATTCAACAATGTT+TGG + chr5.2:51338502-51338521 None:intergenic 30.0%
AACTCTATCATAGGAATGAA+GGG + chr5.2:51338461-51338480 None:intergenic 30.0%
AGTATGTATATGCACATCAA+AGG + chr5.2:51338212-51338231 None:intergenic 30.0%
ATGAAATGCGAGAATAAAAG+AGG - chr5.2:51337994-51338013 MS.gene73969:CDS 30.0%
CATGATCATTTATCTCTATC+AGG - chr5.2:51338551-51338570 MS.gene73969:CDS 30.0%
GAGAATTTAAGTCAAGCTTA+GGG + chr5.2:51335906-51335925 None:intergenic 30.0%
TACACAACATGAGAAGAATT+CGG - chr5.2:51336679-51336698 MS.gene73969:intron 30.0%
TGAAATGCGAGAATAAAAGA+GGG - chr5.2:51337995-51338014 MS.gene73969:CDS 30.0%
TTCAAACTACTGAGGTTATT+TGG + chr5.2:51338662-51338681 None:intergenic 30.0%
TTGATACTGTAATCAGATGA+TGG - chr5.2:51337258-51337277 MS.gene73969:intron 30.0%
TTGCAGTTGATCCATAATTA+TGG + chr5.2:51337887-51337906 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+GGG - chr5.2:51336006-51336025 MS.gene73969:intron 30.0%
! CAAAAGACACCTTTTTACTA+CGG - chr5.2:51336005-51336024 MS.gene73969:intron 30.0%
! GTGGAGCAAATAAATTTTGT+TGG + chr5.2:51336941-51336960 None:intergenic 30.0%
! GTTGAATAATGTTTTCAGCA+AGG + chr5.2:51337380-51337399 None:intergenic 30.0%
! TTTGGTTCAAAGATCTCTTA+GGG + chr5.2:51338484-51338503 None:intergenic 30.0%
!! ACCTATTTGAAGCTCTAAAA+AGG + chr5.2:51336915-51336934 None:intergenic 30.0%
!! TAAACTTTAGAACGTACCTT+TGG + chr5.2:51335571-51335590 None:intergenic 30.0%
!!! AAAAAAAAGTTTTTCGGGAG+TGG + chr5.2:51336831-51336850 None:intergenic 30.0%
!!! AAAAAAAGTTTTTCGGGAGT+GGG + chr5.2:51336830-51336849 None:intergenic 30.0%
!!! AATTGTGGTTATTTTTCAGC+AGG - chr5.2:51338512-51338531 MS.gene73969:CDS 30.0%
!!! ACCTTTTTAGAGCTTCAAAT+AGG - chr5.2:51336911-51336930 MS.gene73969:intron 30.0%
!!! CCCGTTAATTTACTGTTTTT+TGG - chr5.2:51337738-51337757 MS.gene73969:intron 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG + chr5.2:51336006-51336025 None:intergenic 30.0%
AAAACACGACTCTTTAGACT+TGG + chr5.2:51337636-51337655 None:intergenic 35.0%
AACAAAGAGGACGAATTAAG+AGG + chr5.2:51337094-51337113 None:intergenic 35.0%
AACACTAAAAAACTCGTTGC+TGG - chr5.2:51336045-51336064 MS.gene73969:intron 35.0%
AACAGCAACACAAAAGAAGT+TGG - chr5.2:51338713-51338732 MS.gene73969:CDS 35.0%
AATCATATGAGGAGCAAGAA+GGG + chr5.2:51337965-51337984 None:intergenic 35.0%
AATTTCATTGGATCCCTTAG+AGG + chr5.2:51338822-51338841 None:intergenic 35.0%
ACATTAGAGATTGTTCCTTG+AGG + chr5.2:51336459-51336478 None:intergenic 35.0%
AGATCTGATTCTAATAACGG+CGG - chr5.2:51335841-51335860 MS.gene73969:CDS 35.0%
AGTGAATAATAAGAAGGAGG+AGG - chr5.2:51336158-51336177 MS.gene73969:intron 35.0%
AGTTCTTGTCCTTCATTTAC+CGG + chr5.2:51335757-51335776 None:intergenic 35.0%
ATCAACCACTCAATCATATG+AGG + chr5.2:51337976-51337995 None:intergenic 35.0%
ATGATGCTATGAAGATTGGT+TGG - chr5.2:51336361-51336380 MS.gene73969:intron 35.0%
CATGATTCATTTAGACGTTG+AGG - chr5.2:51336618-51336637 MS.gene73969:intron 35.0%
CTCAACAAAATCTATCCCAA+AGG + chr5.2:51338687-51338706 None:intergenic 35.0%
GAAATAAGTTCAACTTGCCA+AGG + chr5.2:51338347-51338366 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG + chr5.2:51338462-51338481 None:intergenic 35.0%
GAAGACTCATCAATCTTCAA+AGG + chr5.2:51336411-51336430 None:intergenic 35.0%
GAGTGAAGTGATAGATAACT+TGG - chr5.2:51337562-51337581 MS.gene73969:intron 35.0%
GATAAGCTTCAGGTATCTTA+TGG - chr5.2:51338046-51338065 MS.gene73969:CDS 35.0%
GCAAGAGAATTATGTGACTA+TGG - chr5.2:51337235-51337254 MS.gene73969:intron 35.0%
GGAGAATTTAAGTCAAGCTT+AGG + chr5.2:51335907-51335926 None:intergenic 35.0%
GGTATATATGTTTCAGCAAG+AGG - chr5.2:51337537-51337556 MS.gene73969:intron 35.0%
GTGAATAATAAGAAGGAGGA+GGG - chr5.2:51336159-51336178 MS.gene73969:intron 35.0%
GTGAATTGAAACTTGTGTAC+CGG + chr5.2:51337202-51337221 None:intergenic 35.0%
TAGAGATAAATGATCATGCC+TGG + chr5.2:51338549-51338568 None:intergenic 35.0%
TATATTACCCTAGGGCTAAT+TGG - chr5.2:51336307-51336326 MS.gene73969:intron 35.0%
TCAGTAGTTTGAACACCTTT+GGG - chr5.2:51338669-51338688 MS.gene73969:CDS 35.0%
TCTTGTAAGAGTCTTTACGA+AGG + chr5.2:51335824-51335843 None:intergenic 35.0%
TGATCTTCACACACTTATCT+CGG - chr5.2:51337114-51337133 MS.gene73969:intron 35.0%
TGTGTGAAGATCAAACAAAG+AGG + chr5.2:51337107-51337126 None:intergenic 35.0%
! AACACAAAAGAAGTTGGTTC+TGG - chr5.2:51338719-51338738 MS.gene73969:CDS 35.0%
! CAGCAAACTTCATATTTCTG+CGG + chr5.2:51336122-51336141 None:intergenic 35.0%
! GCAGAATCCTGAATTAGTTT+GGG + chr5.2:51338413-51338432 None:intergenic 35.0%
! GTTGTTGATGAAGAAGAAGA+CGG - chr5.2:51335784-51335803 MS.gene73969:CDS 35.0%
! GTTTGGTTCAAAGATCTCTT+AGG + chr5.2:51338485-51338504 None:intergenic 35.0%
! GTTTTCATGAAAAACACTGC+TGG - chr5.2:51336078-51336097 MS.gene73969:intron 35.0%
! TGATGCTATGAAGATTGGTT+GGG - chr5.2:51336362-51336381 MS.gene73969:intron 35.0%
! TGTAGTTGATGCAAATGTTG+TGG - chr5.2:51336338-51336357 MS.gene73969:intron 35.0%
!! TTTGCGGTACTTTTAGGAAT+TGG + chr5.2:51337179-51337198 None:intergenic 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG - chr5.2:51336441-51336460 MS.gene73969:intron 35.0%
!!! CGTAGTAAAAAGGTGTCTTT+TGG + chr5.2:51336007-51336026 None:intergenic 35.0%
AAATTGCACCAATTAGCCCT+AGG + chr5.2:51336318-51336337 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+GGG + chr5.2:51337610-51337629 None:intergenic 40.0%
AAGGGAGAAGATTCCTCTAA+GGG - chr5.2:51338806-51338825 MS.gene73969:CDS 40.0%
AATTCGCATTTCGCGTTGTT+AGG - chr5.2:51336647-51336666 MS.gene73969:intron 40.0%
AATTGCACCAATTAGCCCTA+GGG + chr5.2:51336317-51336336 None:intergenic 40.0%
ACTGCTGGAGATATAGTTGT+TGG - chr5.2:51336093-51336112 MS.gene73969:intron 40.0%
ACTTGGAGCAACAATGATCA+AGG + chr5.2:51337619-51337638 None:intergenic 40.0%
AGCCAATCTCCATGAATTAC+AGG - chr5.2:51337139-51337158 MS.gene73969:intron 40.0%
AGGACGGTAACCATCAATAA+CGG + chr5.2:51335653-51335672 None:intergenic 40.0%
ATTCGCATTTCGCGTTGTTA+GGG - chr5.2:51336648-51336667 MS.gene73969:intron 40.0%
ATTGTTTGAGCATTAGGGGA+AGG + chr5.2:51335628-51335647 None:intergenic 40.0%
CAAACAATCGCCGTTATTGA+TGG - chr5.2:51335640-51335659 MS.gene73969:CDS 40.0%
CAATACTATGAATCGATGCG+AGG + chr5.2:51336723-51336742 None:intergenic 40.0%
CAATCATATGAGGAGCAAGA+AGG + chr5.2:51337966-51337985 None:intergenic 40.0%
CCAATCTCCATGAATTACAG+GGG - chr5.2:51337141-51337160 MS.gene73969:intron 40.0%
CTCAGTAGTTTGAACACCTT+TGG - chr5.2:51338668-51338687 MS.gene73969:CDS 40.0%
CTCTTAGGGAACTCTATCAT+AGG + chr5.2:51338470-51338489 None:intergenic 40.0%
GAAAGTGATGTCCATGATTC+TGG - chr5.2:51338779-51338798 MS.gene73969:CDS 40.0%
GCCAATCTCCATGAATTACA+GGG - chr5.2:51337140-51337159 MS.gene73969:intron 40.0%
GGCAGAATCCTGAATTAGTT+TGG + chr5.2:51338414-51338433 None:intergenic 40.0%
GGCAGTGAATAATAAGAAGG+AGG - chr5.2:51336155-51336174 MS.gene73969:intron 40.0%
GTGGATGATGCTATGAAGAT+TGG - chr5.2:51336357-51336376 MS.gene73969:intron 40.0%
TCCGTCATGAACATCAGTAA+CGG + chr5.2:51335950-51335969 None:intergenic 40.0%
TCCGTTACTGATGTTCATGA+CGG - chr5.2:51335946-51335965 MS.gene73969:CDS 40.0%
TCTTCTTCATCAACAACCTC+AGG + chr5.2:51335781-51335800 None:intergenic 40.0%
TGAAGAAGAAGACGGTGATA+AGG - chr5.2:51335792-51335811 MS.gene73969:CDS 40.0%
TTCCTTTCGCTGTATCATCA+CGG - chr5.2:51335672-51335691 MS.gene73969:CDS 40.0%
TTGCTCCTCATATGATTGAG+TGG - chr5.2:51337968-51337987 MS.gene73969:CDS 40.0%
TTGTTTGAGCATTAGGGGAA+GGG + chr5.2:51335627-51335646 None:intergenic 40.0%
! AAAGAGGGACAAGTGTTTTC+TGG - chr5.2:51338010-51338029 MS.gene73969:CDS 40.0%
! AAATTCTCCTTTTCCGTCTC+AGG - chr5.2:51335918-51335937 MS.gene73969:CDS 40.0%
! AAGTTTGCTCATGTCTATCG+AGG - chr5.2:51335976-51335995 MS.gene73969:CDS 40.0%
! ACCCTTGTTATCACCAAACA+AGG + chr5.2:51338435-51338454 None:intergenic 40.0%
! ACTGATTCCCCTGTAATTCA+TGG + chr5.2:51337151-51337170 None:intergenic 40.0%
! AGTTTGCTCATGTCTATCGA+GGG - chr5.2:51335977-51335996 MS.gene73969:CDS 40.0%
! ATTCAGGATTCTGCCTTGTT+TGG - chr5.2:51338419-51338438 MS.gene73969:CDS 40.0%
! GTTTTCAGCAAGGATTGTGT+TGG + chr5.2:51337370-51337389 None:intergenic 40.0%
!! CAAAGGTGTTCAAACTACTG+AGG + chr5.2:51338670-51338689 None:intergenic 40.0%
!! CTCGCATCGATTCATAGTAT+TGG - chr5.2:51336721-51336740 MS.gene73969:intron 40.0%
!! GCCTTGTTTGGTGATAACAA+GGG - chr5.2:51338431-51338450 MS.gene73969:CDS 40.0%
!! GTTGTGATTGTGAGCATTGA+AGG + chr5.2:51337452-51337471 None:intergenic 40.0%
!! TATTTTTCAGCAGGCATGCA+GGG - chr5.2:51338521-51338540 MS.gene73969:CDS 40.0%
!! TGAGAAGAATTCGGTGCATA+TGG - chr5.2:51336688-51336707 MS.gene73969:intron 40.0%
!! TGCCTTGTTTGGTGATAACA+AGG - chr5.2:51338430-51338449 MS.gene73969:CDS 40.0%
!! TTATTTTTCAGCAGGCATGC+AGG - chr5.2:51338520-51338539 MS.gene73969:CDS 40.0%
!! TTCCTAAAAGTACCGCAAAC+CGG - chr5.2:51337180-51337199 MS.gene73969:intron 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG - chr5.2:51336504-51336523 MS.gene73969:intron 40.0%
!!! GGCTTTTTTGTTGAGAGGAA+TGG + chr5.2:51338392-51338411 None:intergenic 40.0%
!!! GTTTGGGCTTTTTTGTTGAG+AGG + chr5.2:51338397-51338416 None:intergenic 40.0%
AAATTCTGGCAGACATGTGC+CGG - chr5.2:51335529-51335548 MS.gene73969:CDS 45.0%
AAGGAGGAGGGTAAAGAGAA+TGG - chr5.2:51336171-51336190 MS.gene73969:intron 45.0%
AAGGGGAATACTAACCGAGA+AGG - chr5.2:51336233-51336252 MS.gene73969:intron 45.0%
ACAATGATCAAGGCTCTCAG+GGG + chr5.2:51337609-51337628 None:intergenic 45.0%
ACGGCGATTGTTTGAGCATT+AGG + chr5.2:51335634-51335653 None:intergenic 45.0%
AGGAATGGTAGATGCACTGT+AGG + chr5.2:51338377-51338396 None:intergenic 45.0%
ATCAAAACCGCGTCAATCCT+TGG - chr5.2:51338327-51338346 MS.gene73969:CDS 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG - chr5.2:51336475-51336494 MS.gene73969:intron 45.0%
CAACAATGATCAAGGCTCTC+AGG + chr5.2:51337611-51337630 None:intergenic 45.0%
CAAGGGAGAAGATTCCTCTA+AGG - chr5.2:51338805-51338824 MS.gene73969:CDS 45.0%
CAATTTGGCGAAGACCTCAT+CGG + chr5.2:51335725-51335744 None:intergenic 45.0%
CATGTTCCAAGTGACCATGT+GGG + chr5.2:51335600-51335619 None:intergenic 45.0%
CCCCTGTAATTCATGGAGAT+TGG + chr5.2:51337144-51337163 None:intergenic 45.0%
CGGCGATTGTTTGAGCATTA+GGG + chr5.2:51335633-51335652 None:intergenic 45.0%
CTACTCAGTCCGGTAAATGA+AGG - chr5.2:51335745-51335764 MS.gene73969:CDS 45.0%
GAGTCCAACCCGTAGTAAAA+AGG + chr5.2:51336017-51336036 None:intergenic 45.0%
GCCAGAATTTGGATTTCACG+CGG + chr5.2:51335519-51335538 None:intergenic 45.0%
GGAAGATCAAGTCAGCACAA+AGG + chr5.2:51335885-51335904 None:intergenic 45.0%
GGAGGGTAAAGAGAATGGTT+TGG - chr5.2:51336176-51336195 MS.gene73969:intron 45.0%
GGAGTTGGCGGAAAAGAATA+CGG - chr5.2:51336272-51336291 MS.gene73969:intron 45.0%
GGCGATTGTTTGAGCATTAG+GGG + chr5.2:51335632-51335651 None:intergenic 45.0%
GTAGATGCACTGTAGGATTG+CGG + chr5.2:51338370-51338389 None:intergenic 45.0%
GTCCATGATTCTGGTAGCAA+GGG - chr5.2:51338788-51338807 MS.gene73969:CDS 45.0%
GTGATGATACAGCGAAAGGA+AGG + chr5.2:51335673-51335692 None:intergenic 45.0%
TACCGGACTGAGTAGCAATT+TGG + chr5.2:51335740-51335759 None:intergenic 45.0%
TAGATGCACTGTAGGATTGC+GGG + chr5.2:51338369-51338388 None:intergenic 45.0%
TCCATCGGTGAATGTTCCAA+AGG - chr5.2:51335552-51335571 MS.gene73969:CDS 45.0%
TCTGATTCTAATAACGGCGG+TGG - chr5.2:51335844-51335863 MS.gene73969:CDS 45.0%
TGAAGGACAAGAACTTCCTG+AGG - chr5.2:51335762-51335781 MS.gene73969:CDS 45.0%
TGATACAGCGAAAGGAAGGA+CGG + chr5.2:51335669-51335688 None:intergenic 45.0%
TGTCCATGATTCTGGTAGCA+AGG - chr5.2:51338787-51338806 MS.gene73969:CDS 45.0%
TTACTTCCCACATGGTCACT+TGG - chr5.2:51335591-51335610 MS.gene73969:CDS 45.0%
TTCGCATTTCGCGTTGTTAG+GGG - chr5.2:51336649-51336668 MS.gene73969:intron 45.0%
! AGAGAATGGTTTGGAGGTCA+AGG - chr5.2:51336185-51336204 MS.gene73969:intron 45.0%
! ATGAAGATTGGTTGGGCATC+TGG - chr5.2:51336369-51336388 MS.gene73969:intron 45.0%
! CTGATGTTCATGACGGAGTT+TGG - chr5.2:51335953-51335972 MS.gene73969:CDS 45.0%
! GACACCTTTTTACTACGGGT+TGG - chr5.2:51336010-51336029 MS.gene73969:intron 45.0%
! GAGAATGGTTTGGAGGTCAA+GGG - chr5.2:51336186-51336205 MS.gene73969:intron 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG - chr5.2:51336179-51336198 MS.gene73969:intron 45.0%
! GGGTTAGGTTATAGGAGTGT+AGG - chr5.2:51337687-51337706 MS.gene73969:intron 45.0%
!! ACCTTTGGAACATTCACCGA+TGG + chr5.2:51335556-51335575 None:intergenic 45.0%
!! GAAACTTGTGTACCGGTTTG+CGG + chr5.2:51337195-51337214 None:intergenic 45.0%
!! GAAGAATTCGGTGCATATGG+AGG - chr5.2:51336691-51336710 MS.gene73969:intron 45.0%
!! TTGTGTTGGTTGAGCTGAGA+CGG + chr5.2:51337356-51337375 None:intergenic 45.0%
!!! TACCGGTTTGCGGTACTTTT+AGG + chr5.2:51337185-51337204 None:intergenic 45.0%
AACCGAGAAGGCGGTTATTG+AGG - chr5.2:51336245-51336264 MS.gene73969:intron 50.0%
ACCTGAAGCATGCGCCAATT+AGG + chr5.2:51336492-51336511 None:intergenic 50.0%
AGGATCGGCTAGGAGATCAA+CGG + chr5.2:51335698-51335717 None:intergenic 50.0%
ATCAAGTCAGCACAAAGGCG+AGG + chr5.2:51335880-51335899 None:intergenic 50.0%
CAACTTGCCAAGGATTGACG+CGG + chr5.2:51338337-51338356 None:intergenic 50.0%
CACAATAGCAGCAGCAACAG+TGG - chr5.2:51337516-51337535 MS.gene73969:intron 50.0%
CACTGTTGCTGCTGCTATTG+TGG + chr5.2:51337518-51337537 None:intergenic 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr5.2:51336491-51336510 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG - chr5.2:51335735-51335754 MS.gene73969:CDS 50.0%
CTCCCTTGCTACCAGAATCA+TGG + chr5.2:51338793-51338812 None:intergenic 50.0%
GAGAGAGAGAGAGAGATCTG+CGG + chr5.2:51336963-51336982 None:intergenic 50.0%
GAGAGTTCCTGAGACGGAAA+AGG + chr5.2:51335928-51335947 None:intergenic 50.0%
GCATGTTCCAAGTGACCATG+TGG + chr5.2:51335601-51335620 None:intergenic 50.0%
GCCGCGTGAAATCCAAATTC+TGG - chr5.2:51335515-51335534 MS.gene73969:CDS 50.0%
GGCACATGTCTGCCAGAATT+TGG + chr5.2:51335530-51335549 None:intergenic 50.0%
GGCGACAAGTCGTTAGATGT+GGG + chr5.2:51338641-51338660 None:intergenic 50.0%
GGGAATACTAACCGAGAAGG+CGG - chr5.2:51336236-51336255 MS.gene73969:intron 50.0%
GGTTATTGAGGCAGTGGAGT+TGG - chr5.2:51336257-51336276 MS.gene73969:intron 50.0%
TATTGAGGCAGTGGAGTTGG+CGG - chr5.2:51336260-51336279 MS.gene73969:intron 50.0%
TGCCTCAATAACCGCCTTCT+CGG + chr5.2:51336250-51336269 None:intergenic 50.0%
TGGAACATTCACCGATGGAC+CGG + chr5.2:51335551-51335570 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG + chr5.2:51338642-51338661 None:intergenic 50.0%
TTCGCGTTGTTAGGGGTTTG+CGG - chr5.2:51336656-51336675 MS.gene73969:intron 50.0%
! AAGGGAGAGGGTTTTTCTCG+AGG - chr5.2:51336204-51336223 MS.gene73969:intron 50.0%
! GTAACGGAGAGTTCCTGAGA+CGG + chr5.2:51335934-51335953 None:intergenic 50.0%
! GTTTGCTGCAGCTGAAGCAA+AGG - chr5.2:51336134-51336153 MS.gene73969:intron 50.0%
! TTTTTCTCGAGGCGGGAGAA+GGG - chr5.2:51336215-51336234 MS.gene73969:intron 50.0%
AGAGAGAGAGAGATCTGCGG+TGG + chr5.2:51336960-51336979 None:intergenic 55.0%
CATGCGCCAATTAGGGACAC+TGG + chr5.2:51336484-51336503 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr5.2:51336488-51336507 MS.gene73969:intron 55.0%
GAAGGCGGTTATTGAGGCAG+TGG - chr5.2:51336251-51336270 MS.gene73969:intron 55.0%
GACCTCATCGGTGTGAGGAT+CGG + chr5.2:51335713-51335732 None:intergenic 55.0%
GGCCGTGATGATACAGCGAA+AGG + chr5.2:51335677-51335696 None:intergenic 55.0%
GGTTTGGAGGTCAAGGGAGA+GGG - chr5.2:51336192-51336211 MS.gene73969:intron 55.0%
TTGAGCATTAGGGGAAGGGC+AGG + chr5.2:51335623-51335642 None:intergenic 55.0%
! GAGAGGGTTTTTCTCGAGGC+GGG - chr5.2:51336208-51336227 MS.gene73969:intron 55.0%
! GGAGAGGGTTTTTCTCGAGG+CGG - chr5.2:51336207-51336226 MS.gene73969:intron 55.0%
! GTTTTTCTCGAGGCGGGAGA+AGG - chr5.2:51336214-51336233 MS.gene73969:intron 55.0%
! TGAGGAGCAAGAAGGGTGCA+TGG + chr5.2:51337958-51337977 None:intergenic 55.0%
! TGGTTTGGAGGTCAAGGGAG+AGG - chr5.2:51336191-51336210 MS.gene73969:intron 55.0%
! TTTTCTCGAGGCGGGAGAAG+GGG - chr5.2:51336216-51336235 MS.gene73969:intron 55.0%
AGCCGATCCTCACACCGATG+AGG - chr5.2:51335708-51335727 MS.gene73969:CDS 60.0%
CATCGGTGTGAGGATCGGCT+AGG + chr5.2:51335708-51335727 None:intergenic 60.0%
GCGAAGACCTCATCGGTGTG+AGG + chr5.2:51335718-51335737 None:intergenic 60.0%
TTGGATTTCACGCGGCGTGC+CGG + chr5.2:51335511-51335530 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG - chr5.2:51335537-51335556 MS.gene73969:CDS 60.0%
ATTTCACGCGGCGTGCCGGA+GGG + chr5.2:51335507-51335526 None:intergenic 65.0%
GACAGACATGGTGGCCGGTG+GGG + chr5.2:51335485-51335504 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG + chr5.2:51335486-51335505 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG + chr5.2:51335490-51335509 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG - chr5.2:51335489-51335508 MS.gene73969:CDS 65.0%
TGCCGGAGGGACAGACATGG+TGG + chr5.2:51335494-51335513 None:intergenic 65.0%
CAGCAGGCATGCAGGGAGCC+AGG - chr5.2:51338528-51338547 MS.gene73969:CDS 70.0%
GATTTCACGCGGCGTGCCGG+AGG + chr5.2:51335508-51335527 None:intergenic 70.0%
GCGTGCCGGAGGGACAGACA+TGG + chr5.2:51335497-51335516 None:intergenic 70.0%
GGGACAGACATGGTGGCCGG+TGG + chr5.2:51335487-51335506 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 51335475 51338844 51335475 ID=MS.gene73969
chr5.2 mRNA 51335475 51338844 51335475 ID=MS.gene73969.t1;Parent=MS.gene73969
chr5.2 exon 51337810 51338844 51337810 ID=MS.gene73969.t1.exon1;Parent=MS.gene73969.t1
chr5.2 CDS 51337810 51338844 51337810 ID=cds.MS.gene73969.t1;Parent=MS.gene73969.t1
chr5.2 exon 51335475 51336023 51335475 ID=MS.gene73969.t1.exon2;Parent=MS.gene73969.t1
chr5.2 CDS 51335475 51336023 51335475 ID=cds.MS.gene73969.t1;Parent=MS.gene73969.t1
Gene Sequence

>MS.gene73969

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCACGCCGCGTGAAATCCAAATTCTGGCAGACATGTGCCGGTCCATCGGTGAATGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACATGGTCACTTGGAACATGCCTGCCCTTCCCCTAATGCTCAAACAATCGCCGTTATTGATGGTTACCGTCCTTCCTTTCGCTGTATCATCACGGCCGTTGATCTCCTAGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAAATGAAGGACAAGAACTTCCTGAGGTTGTTGATGAAGAAGAAGACGGTGATAAGGTTGTTTCCTTCGTAAAGACTCTTACAAGATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCTAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACGGGTTGGACTCCGTTTGTGAACACTAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCTGGAGATATAGTTGTTGGAATCCGCAGAAATATGAAGTTTGCTGCAGCTGAAGCAAAGGCAGTGAATAATAAGAAGGAGGAGGGTAAAGAGAATGGTTTGGAGGTCAAGGGAGAGGGTTTTTCTCGAGGCGGGAGAAGGGGAATACTAACCGAGAAGGCGGTTATTGAGGCAGTGGAGTTGGCGGAAAAGAATACGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTTGATGCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAGTCTTCAAATTCAAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTGCTCTTCTTTTTTGGTATTATCGTAGTTTTATTTTATTTTTTGAATCAAGGGCTGATTATATGTGACTGCATAATGTTATAAATATTATTGTCGAAGAAGTTTAGGCATGATTCATTTAGACGTTGAGGTCACATAATTCGCATTTCGCGTTGTTAGGGGTTTGCGGTACACAACATGAGAAGAATTCGGTGCATATGGAGGTATAAACCTCGCATCGATTCATAGTATTGGTACTACCTAATTAAGTATACTAAACTATCTTAATATTACAAACAATTTGAAGGATTATATCGACAAGCTACAACAAATTAGTGCCCACTCCCGAAAAACTTTTTTTTACACTCACTTTTCTTTCACCTTTTTACTTTTGTATCAATGTTATTTTTCTAGAGACGTTCACCTTTTTAGAGCTTCAAATAGGTTTTCCAACAAAATTTATTTGCTCCACCGCAGATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTCTTATTCAACCTCTTAATTCGTCCTCTTTGTTTGATCTTCACACACTTATCTCGGTTAGCCAATCTCCATGAATTACAGGGGAATCAGTGCGAACCAATTCCTAAAAGTACCGCAAACCGGTACACAAGTTTCAATTCACAATTCGCAGCATTGCAAGAGAATTATGTGACTATGGTTGATACTGTAATCAGATGATGGTTCATGAAATAGATGTCCAAAATTCTAATACAAGTAATTATTAATGTTTAATATTAGTATTCAATCATCTAACCGTCTCAGCTCAACCAACACAATCCTTGCTGAAAACATTATTCAACAATTATGTGACATGATTACCAAAATACAGTTAGGAAAAATCACAACAAACCTTCAATGCTCACAATCACAACAATAGTTAGCTGAATAATTATAGTAGTGTCATCATTTTTCTCTCCACAATAGCAGCAGCAACAGTGGTATATATGTTTCAGCAAGAGGAAGAGTGAAGTGATAGATAACTTGGAATGCAAACATAGAGTTACATCCCCTGAGAGCCTTGATCATTGTTGCTCCAAGTCTAAAGAGTCGTGTTTTATCCCTTGATCAATTTAGTATACAAATATTAGGGTTAGGTTATAGGAGTGTAGGCTAGTATACTATGTATAGCTTTGTTGTGCCCGTTAATTTACTGTTTTTTGGTGTTGTCATTATTTACCTTCAACACTAATATAAAAAGGAAATATTAGAACATCAATTTATTACAATAGAGCTAATTTTAAGAGAATACATTTGAAGTATTTTATAGTTGATACCAAAATTAATCCATAATTATGGATCAACTGCAACACAATTCATTCTTGCACTTGTATCATTTGAGATCATTAGATGCGAAACCATGCACCCTTCTTGCTCCTCATATGATTGAGTGGTTGATGAAATGCGAGAATAAAAGAGGGACAAGTGTTTTCTGGCATATAAATAATGGATAAGCTTCAGGTATCTTATGGAAAAGTATTAGTTATTTAGGTTACCATATTTTTATAATAAAATGCATTTTCTCATATTCCTAATTTTATAATAAAATGCATTTGCTCATTTTCTTAAGTAGGATCTATATTTTACTCACATGTTAGTATTTGTTAAGAGACCTTTGATGTGCATATACATACTATAACTTATCGTATATTTTGAATAACTGTATTGGATTCTTACGATTTTTATTTCTTTATCATAGGTTAACTGGGATGAACTCGAAATCTCGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCGCAATCCTACAGTGCATCTACCATTCCTCTCAACAAAAAAGCCCAAACTAATTCAGGATTCTGCCTTGTTTGGTGATAACAAGGGTGATCCCTTCATTCCTATGATAGAGTTCCCTAAGAGATCTTTGAACCAAACATTGTTGAATTGTGGTTATTTTTCAGCAGGCATGCAGGGAGCCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACCTCAGTAGTTTGAACACCTTTGGGATAGATTTTGTTGAGACTCACAACAGCAACACAAAAGAAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACTGTTGAAAGTGATGTCCATGATTCTGGTAGCAAGGGAGAAGATTCCTCTAAGGGATCCAATGAAATTTGA
Protein sequence

>MS.gene73969.t1

MSSPPATMSVPPARRVKSKFWQTCAGPSVNVPKVRSKVYYFPHGHLEHACPSPNAQTIAVIDGYRPSFRCIITAVDLLADPHTDEVFAKLLLSPVNEGQELPEVVDEEEDGDKVVSFVKTLTRSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFAAAEAKAVNNKKEEGKENGLEVKGEGFSRGGRRGILTEKAVIEAVELAEKNTAFEVIYYPRANWCNFVVDANVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEISQNQNRVNPWQVELISRNPTVHLPFLSTKKPKLIQDSALFGDNKGDPFIPMIEFPKRSLNQTLLNCGYFSAGMQGARHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNLSSLNTFGIDFVETHNSNTKEVGSGSIKLFGKIIQTVESDVHDSGSKGEDSSKGSNEI