AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74009

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74009.t1 MTR_5g058860 97.831 415 6 2 1 414 94 506 0.0 835
MS.gene74009.t1 MTR_3g072710 68.949 409 100 9 15 408 107 503 0.0 538
MS.gene74009.t1 MTR_7g027190 36.691 417 210 17 14 406 288 674 4.43e-63 215
MS.gene74009.t1 MTR_8g093070 37.065 402 215 14 13 405 124 496 8.16e-63 210
MS.gene74009.t1 MTR_3g065980 34.069 408 210 15 22 404 166 539 1.17e-52 184
MS.gene74009.t1 MTR_7g074650 35.294 374 215 11 33 404 443 791 2.70e-52 187
MS.gene74009.t1 MTR_1g069725 32.754 403 238 10 6 406 93 464 3.47e-48 171
MS.gene74009.t1 MTR_4g076020 35.989 364 188 13 57 412 110 436 1.58e-47 168
MS.gene74009.t1 MTR_5g097480 30.848 389 241 11 25 404 174 543 2.67e-44 162
MS.gene74009.t1 MTR_1g096030 31.401 414 238 13 18 407 70 461 4.35e-43 157
MS.gene74009.t1 MTR_3g056110 31.781 365 223 10 49 404 194 541 1.23e-42 157
MS.gene74009.t1 MTR_8g442410 31.910 398 236 13 21 404 148 524 4.46e-42 155
MS.gene74009.t1 MTR_1g029420 31.606 386 238 14 27 404 224 591 1.54e-41 155
MS.gene74009.t1 MTR_2g082090 30.941 404 244 14 15 404 196 578 1.83e-41 154
MS.gene74009.t1 MTR_4g104020 29.440 411 258 10 9 406 130 521 2.49e-40 150
MS.gene74009.t1 MTR_4g077760 31.609 348 222 6 59 404 221 554 2.64e-40 151
MS.gene74009.t1 MTR_3g089055 30.130 385 245 11 27 404 202 569 1.44e-39 149
MS.gene74009.t1 MTR_3g089055 30.130 385 245 11 27 404 197 564 1.45e-39 149
MS.gene74009.t1 MTR_4g102790 30.542 406 237 17 21 407 285 664 6.57e-39 148
MS.gene74009.t1 MTR_4g133660 29.972 357 225 10 59 406 204 544 2.79e-38 145
MS.gene74009.t1 MTR_2g026250 26.891 357 236 10 59 406 257 597 1.39e-34 135
MS.gene74009.t1 MTR_5g094450 28.827 392 249 11 25 404 158 531 4.76e-34 133
MS.gene74009.t1 MTR_2g097410 27.363 402 253 13 20 405 363 741 1.44e-32 130
MS.gene74009.t1 MTR_4g064200 27.877 391 245 15 28 405 284 650 4.35e-30 122
MS.gene74009.t1 MTR_0092s0100 29.592 392 235 15 25 406 369 729 5.24e-30 122
MS.gene74009.t1 MTR_0092s0100 29.592 392 235 15 25 406 372 732 5.51e-30 122
MS.gene74009.t1 MTR_2g097467 26.891 357 232 9 58 404 316 653 1.49e-29 121
MS.gene74009.t1 MTR_4g064150 28.680 394 243 13 28 405 362 733 4.82e-29 120
MS.gene74009.t1 MTR_2g034250 24.818 411 261 10 17 404 199 584 3.01e-28 117
MS.gene74009.t1 MTR_3g022830 23.902 410 225 9 57 404 51 435 4.91e-28 115
MS.gene74009.t1 MTR_2g097473 26.536 358 234 9 58 405 316 654 1.62e-27 115
MS.gene74009.t1 MTR_8g077940 29.067 375 234 13 40 406 192 542 1.84e-27 114
MS.gene74009.t1 MTR_4g064120 25.178 421 271 12 5 407 231 625 3.14e-27 114
MS.gene74009.t1 MTR_2g034260 25.558 403 271 13 13 404 199 583 5.78e-27 113
MS.gene74009.t1 MTR_2g097463 26.536 358 234 10 58 405 317 655 7.16e-27 113
MS.gene74009.t1 MTR_7g062120 25.063 395 259 11 23 404 369 739 9.98e-27 113
MS.gene74009.t1 MTR_4g097080 26.562 384 263 10 27 404 132 502 3.55e-26 110
MS.gene74009.t1 MTR_2g099110 30.000 330 199 12 1 318 43 352 4.93e-26 109
MS.gene74009.t1 MTR_4g064180 25.708 424 265 15 5 407 231 625 6.60e-26 110
MS.gene74009.t1 MTR_4g064160 25.542 415 273 14 5 405 292 684 2.23e-25 108
MS.gene74009.t1 MTR_2g097390 26.615 387 253 13 28 404 275 640 8.16e-25 107
MS.gene74009.t1 MTR_4g076140 32.051 234 135 6 14 243 34 247 1.24e-24 105
MS.gene74009.t1 MTR_5g009080 31.760 233 135 7 15 243 43 255 5.62e-24 103
MS.gene74009.t1 MTR_2g034280 24.250 400 278 11 13 404 192 574 7.12e-24 104
MS.gene74009.t1 MTR_5g015490 27.930 401 242 16 27 404 113 489 7.63e-24 103
MS.gene74009.t1 MTR_2g097350 25.831 391 251 15 28 404 274 639 3.34e-23 102
MS.gene74009.t1 MTR_2g097310 24.163 418 277 12 8 405 241 638 3.75e-23 102
MS.gene74009.t1 MTR_2g097380 24.370 357 215 10 58 405 251 561 4.80e-22 98.6
MS.gene74009.t1 MTR_7g109580 24.129 402 265 15 17 404 178 553 2.70e-21 96.3
MS.gene74009.t1 MTR_4g026485 25.195 385 252 14 29 406 270 625 1.81e-20 94.0
MS.gene74009.t1 MTR_7g069740 26.265 415 255 18 9 404 201 583 4.28e-16 80.5
MS.gene74009.t1 MTR_2g089100 24.249 433 283 13 6 405 23 443 1.07e-14 75.9
MS.gene74009.t1 MTR_1g086970 22.005 409 278 12 17 407 95 480 2.90e-14 74.7
MS.gene74009.t1 MTR_7g057230 27.097 310 197 10 106 404 365 656 5.82e-14 74.3
MS.gene74009.t1 MTR_8g020840 23.929 397 264 14 27 404 170 547 1.12e-12 70.1
MS.gene74009.t1 MTR_4g095500 24.533 428 255 18 27 404 58 467 1.11e-11 66.6
MS.gene74009.t1 MTR_6g047750 25.381 394 248 15 29 404 296 661 3.79e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74009.t1 AT4G08250 56.599 394 158 7 14 404 93 476 7.49e-146 424
MS.gene74009.t1 AT3G54220 36.476 403 224 13 12 408 277 653 2.41e-55 194
MS.gene74009.t1 AT5G41920 36.070 402 219 14 12 404 27 399 3.44e-53 182
MS.gene74009.t1 AT3G03450 34.103 390 228 13 22 404 177 544 1.71e-51 181
MS.gene74009.t1 AT5G17490 35.088 399 224 15 19 410 151 521 3.88e-50 177
MS.gene74009.t1 AT1G66350 32.234 394 219 15 22 404 149 505 3.29e-46 166
MS.gene74009.t1 AT2G01570 32.997 397 228 16 17 404 213 580 2.80e-45 165
MS.gene74009.t1 AT1G14920 31.566 396 235 14 17 404 161 528 3.30e-44 161
MS.gene74009.t1 AT5G48150 31.070 383 241 10 29 404 123 489 1.29e-42 156
MS.gene74009.t1 AT5G48150 31.070 383 241 10 29 404 123 489 1.29e-42 156
MS.gene74009.t1 AT5G48150 31.070 383 241 10 29 404 123 489 1.29e-42 156
MS.gene74009.t1 AT5G48150 31.070 383 241 10 29 404 123 489 1.29e-42 156
MS.gene74009.t1 AT2G04890 31.139 395 241 13 16 404 43 412 9.24e-42 152
MS.gene74009.t1 AT1G55580 31.771 384 209 10 61 404 74 444 8.84e-41 150
MS.gene74009.t1 AT4G17230 32.494 397 228 12 23 404 153 524 8.93e-41 152
MS.gene74009.t1 AT4G17230 32.494 397 228 12 23 404 153 524 8.93e-41 152
MS.gene74009.t1 AT1G50600 30.939 362 230 8 49 404 178 525 1.26e-40 151
MS.gene74009.t1 AT1G50600 31.507 365 224 10 49 404 249 596 4.83e-40 150
MS.gene74009.t1 AT1G50600 31.215 362 229 8 49 404 253 600 5.44e-40 150
MS.gene74009.t1 AT5G66770 30.025 403 265 9 5 404 195 583 1.03e-38 147
MS.gene74009.t1 AT3G50650 31.694 366 220 10 50 404 195 541 4.95e-38 144
MS.gene74009.t1 AT1G63100 29.268 410 246 13 18 405 242 629 2.24e-34 135
MS.gene74009.t1 AT1G63100 29.268 410 246 13 18 405 267 654 2.62e-34 135
MS.gene74009.t1 AT1G21450 28.792 389 246 13 27 404 224 592 2.31e-32 129
MS.gene74009.t1 AT4G37650 28.283 396 252 12 28 404 146 528 2.96e-30 122
MS.gene74009.t1 AT4G36710 29.380 371 227 13 44 404 139 484 1.27e-28 117
MS.gene74009.t1 AT3G60630 28.362 409 261 11 13 406 232 623 2.89e-28 117
MS.gene74009.t1 AT3G46600 26.303 403 237 13 27 404 3 370 3.39e-28 114
MS.gene74009.t1 AT2G29065 27.318 399 247 11 28 405 252 628 3.73e-28 117
MS.gene74009.t1 AT3G46600 26.303 403 237 13 27 404 211 578 1.25e-27 115
MS.gene74009.t1 AT3G46600 25.829 422 251 14 8 404 162 546 1.71e-27 114
MS.gene74009.t1 AT1G07530 26.585 410 269 13 7 405 377 765 7.09e-27 113
MS.gene74009.t1 AT2G29060 26.316 399 256 14 25 407 281 657 1.22e-26 112
MS.gene74009.t1 AT2G29060 26.316 399 256 14 25 407 318 694 1.38e-26 112
MS.gene74009.t1 AT5G59450 26.131 398 252 12 25 404 224 597 7.45e-26 110
MS.gene74009.t1 AT2G45160 27.543 403 241 16 25 406 268 640 7.97e-25 107
MS.gene74009.t1 AT1G07520 24.823 423 278 9 2 405 128 529 9.35e-25 106
MS.gene74009.t1 AT1G07520 24.823 423 278 9 2 405 128 529 9.35e-25 106
MS.gene74009.t1 AT1G07520 24.700 417 286 8 2 405 292 693 1.66e-24 106
MS.gene74009.t1 AT1G07520 24.700 417 286 8 2 405 293 694 1.72e-24 106
MS.gene74009.t1 AT4G00150 28.198 383 238 12 29 408 209 557 1.07e-23 103
MS.gene74009.t1 AT1G50420 32.174 230 132 7 19 243 48 258 3.65e-23 101
MS.gene74009.t1 AT1G50420 30.263 152 103 3 256 406 331 480 2.69e-12 68.6
MS.gene74009.t1 AT2G37650 25.450 389 261 11 25 404 344 712 1.21e-22 100
MS.gene74009.t1 AT2G37650 25.450 389 261 11 25 404 332 700 1.28e-22 100
MS.gene74009.t1 AT5G52510 27.554 323 201 10 102 404 330 639 9.55e-17 82.8
MS.gene74009.t1 AT3G49950 25.000 400 263 14 27 407 29 410 2.78e-15 77.4
MS.gene74009.t1 AT3G13840 25.558 403 269 11 10 404 130 509 1.45e-14 75.9

Find 137 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGTTTCCTATGAATAAA+TGG 0.181822 5.3:-46659505 MS.gene74009:CDS
GACTCTTGTTTGTGCCTGTT+AGG 0.230972 5.3:+46660311 None:intergenic
TTGTCAAGCAAAGTTGTTAT+TGG 0.254379 5.3:-46659321 MS.gene74009:CDS
GTTTATTTAACGATGGGTAT+AGG 0.263361 5.3:-46659298 MS.gene74009:CDS
TTTATTTAACGATGGGTATA+GGG 0.268630 5.3:-46659297 MS.gene74009:CDS
CGGTTCCGTTTAGCAGAGTT+TGG 0.274447 5.3:+46660188 None:intergenic
TTTGCCACTGTTCAGGAAAC+TGG 0.295594 5.3:-46659854 MS.gene74009:CDS
AGGAGCAAGAGAGGAGTTCT+TGG 0.324248 5.3:-46659400 MS.gene74009:CDS
ATCGTCCTTTGCCACTGTTC+AGG 0.328568 5.3:-46659861 MS.gene74009:CDS
GGGGAGTGGTTGGGTGTGGA+AGG 0.333319 5.3:-46659377 MS.gene74009:CDS
ATCACTGATGCAGGCTTTGT+GGG 0.334159 5.3:-46659581 MS.gene74009:CDS
CCGTCTAGTGCACCTCCTTA+TGG 0.339880 5.3:-46660347 MS.gene74009:CDS
GGTAACAATAAGTTGGTGTT+AGG 0.342092 5.3:-46659263 MS.gene74009:CDS
TGTCAAGCAAAGTTGTTATT+GGG 0.347692 5.3:-46659320 MS.gene74009:CDS
TTGAACGGTGCCAAGGAGTT+AGG 0.348949 5.3:-46659644 MS.gene74009:CDS
CATCACTGATGCAGGCTTTG+TGG 0.352228 5.3:-46659582 MS.gene74009:CDS
ATTTGCCACATCTTAGTTAC+CGG 0.358640 5.3:-46659691 MS.gene74009:CDS
AGGCTTTGTGGGTTTGTTCA+TGG 0.365262 5.3:-46659570 MS.gene74009:CDS
GTTTGCTTTCTGCTTCTGTT+TGG 0.368542 5.3:-46659226 MS.gene74009:CDS
CTCCTAGCCCATTTATTCAT+AGG 0.371100 5.3:+46659497 None:intergenic
GCGGCAGCGGAGGCCCTAAC+AGG 0.386266 5.3:-46660325 MS.gene74009:CDS
CATAGTGGACTTTGATATCA+TGG 0.386901 5.3:-46660005 MS.gene74009:CDS
ACAATAAGTTGGTGTTAGGT+TGG 0.398047 5.3:-46659259 MS.gene74009:CDS
TTTCATGAGTAACAGCTTCA+AGG 0.400010 5.3:+46660038 None:intergenic
ATGTATGACTCGTTCGAGGC+TGG 0.402016 5.3:-46659524 MS.gene74009:CDS
TGGGTATAGGGTGGAAGAGT+TGG 0.402705 5.3:-46659285 MS.gene74009:CDS
CGTCAAGTTTGCTCATTTCA+CGG 0.410498 5.3:-46660074 MS.gene74009:CDS
ACTCTTGTTTGTGCCTGTTA+GGG 0.415722 5.3:+46660312 None:intergenic
GGGAGTGGTTGGGTGTGGAA+GGG 0.418452 5.3:-46659376 MS.gene74009:CDS
TGTATGACTCGTTCGAGGCT+GGG 0.428573 5.3:-46659523 MS.gene74009:CDS
GGGCCTCCGCTGCCGCCATA+AGG 0.429881 5.3:+46660332 None:intergenic
GGGTATAGGGTGGAAGAGTT+GGG 0.434315 5.3:-46659284 MS.gene74009:CDS
TGCGAAGATGTGGGCCCGGC+AGG 0.434472 5.3:+46659930 None:intergenic
GGAGCAAGAGAGGAGTTCTT+GGG 0.440358 5.3:-46659399 MS.gene74009:CDS
CCTCCTTATGGCGGCAGCGG+AGG 0.448204 5.3:-46660335 MS.gene74009:CDS
GCCACTGAGCCCATTATTCG+TGG 0.453840 5.3:+46659449 None:intergenic
CACGACGGAGGAGGAGGAGG+AGG 0.458831 5.3:-46660389 MS.gene74009:CDS
TCGCGAAAAGAAGGCCTGCC+GGG 0.460380 5.3:-46659944 MS.gene74009:CDS
GGAGGAGGAGGATGACAGAA+AGG 0.460604 5.3:-46660374 MS.gene74009:CDS
AAGCTGTTACTCATGAAAGA+AGG 0.464529 5.3:-46660033 MS.gene74009:CDS
TTCCACGACGGAGGAGGAGG+AGG 0.471274 5.3:-46660392 MS.gene74009:CDS
TAACAGCTTCAAGGATAGCT+TGG 0.472914 5.3:+46660047 None:intergenic
GAGTTTGTGGTGACCACAAT+CGG 0.474218 5.3:+46660168 None:intergenic
CTCCGTCGTGGAATTTGAGT+TGG 0.475797 5.3:+46660402 None:intergenic
ACATTAGTCGAGGAGGAAGT+AGG 0.480496 5.3:-46659608 MS.gene74009:CDS
TATCATGGAAGGGGCCCAAT+GGG 0.484349 5.3:-46659990 MS.gene74009:CDS
GAGCTTTGTTCCTAACTCCT+TGG 0.485022 5.3:+46659634 None:intergenic
GCCCTCCAAACTCTGCTAAA+CGG 0.486218 5.3:-46660193 MS.gene74009:CDS
TGGCTCAAGTGATATTGATT+CGG 0.487459 5.3:-46660282 MS.gene74009:CDS
GCACCTCCTTATGGCGGCAG+CGG 0.487716 5.3:-46660338 MS.gene74009:CDS
TGAGAGTATGTCTGTTTGGT+GGG 0.489093 5.3:+46660129 None:intergenic
ATCGCGAAAAGAAGGCCTGC+CGG 0.491290 5.3:-46659945 MS.gene74009:CDS
ACAACCTTTGCTGCTTCCGT+TGG 0.496161 5.3:-46659821 MS.gene74009:CDS
TCACTTTCATCGCGAAAAGA+AGG 0.496428 5.3:-46659953 MS.gene74009:CDS
TATTGGGTTTATTTAACGAT+GGG 0.498402 5.3:-46659304 MS.gene74009:CDS
CAGTGAGAAGACAGAGTTTG+TGG 0.500911 5.3:+46660155 None:intergenic
TCTTCATTAAAGCTTGTTCG+TGG 0.502342 5.3:-46659743 MS.gene74009:CDS
CCTCCGCTGCCGCCATAAGG+AGG 0.504163 5.3:+46660335 None:intergenic
AGAGTTGGGTAACAATAAGT+TGG 0.507514 5.3:-46659270 MS.gene74009:CDS
AGGCTGAGAGTATGTCTGTT+TGG 0.507765 5.3:+46660125 None:intergenic
CTGCTAAACGGAACCGATTG+TGG 0.507905 5.3:-46660181 MS.gene74009:CDS
TGGGTTTCCTATGAATAAAT+GGG 0.510723 5.3:-46659504 MS.gene74009:CDS
GAGCAAGAGAGGAGTTCTTG+GGG 0.512566 5.3:-46659398 MS.gene74009:CDS
CACAAAGCCTGCATCAGTGA+TGG 0.513600 5.3:+46659583 None:intergenic
TTGAATGAGTGAAGCCCATT+GGG 0.514305 5.3:+46659976 None:intergenic
AGTGATATTGATTCGGCTCA+AGG 0.514796 5.3:-46660275 MS.gene74009:CDS
CACAAACAAGAGTCACCATC+TGG 0.516410 5.3:-46660302 MS.gene74009:CDS
TCCGTTTAGCAGAGTTTGGA+GGG 0.520521 5.3:+46660192 None:intergenic
CTCAAATTCCACGACGGAGG+AGG 0.521407 5.3:-46660398 MS.gene74009:CDS
TTGGGTGTGGAAGGGTTCAA+AGG 0.525480 5.3:-46659368 MS.gene74009:CDS
TTCCGTTTAGCAGAGTTTGG+AGG 0.528085 5.3:+46660191 None:intergenic
ATAACAACTTTGCTTGACAA+TGG 0.528206 5.3:+46659324 None:intergenic
CACAAACTCTGTCTTCTCAC+TGG 0.528238 5.3:-46660154 MS.gene74009:CDS
TGAAAGAAGGGTTCACATAG+TGG 0.529945 5.3:-46660020 MS.gene74009:CDS
TGAGTTGGACATGTCTCCCA+TGG 0.535777 5.3:+46660417 None:intergenic
AAAGGAGTTAACATAAGCTA+TGG 0.536826 5.3:-46659350 MS.gene74009:CDS
TTATTGGGTTTATTTAACGA+TGG 0.539610 5.3:-46659305 MS.gene74009:CDS
CTGAGAGTATGTCTGTTTGG+TGG 0.539651 5.3:+46660128 None:intergenic
AGCGATGTATGACTCGTTCG+AGG 0.539838 5.3:-46659528 MS.gene74009:CDS
GTGAAGAGGAGGAGCAAGAG+AGG 0.542655 5.3:-46659409 MS.gene74009:CDS
GTAGGGCCCATCACTGATGC+AGG 0.543816 5.3:-46659590 MS.gene74009:CDS
GAGGAGGAGGATGACAGAAA+GGG 0.544247 5.3:-46660373 MS.gene74009:CDS
AAATTCCACGACGGAGGAGG+AGG 0.545632 5.3:-46660395 MS.gene74009:CDS
ACAAAGCCTGCATCAGTGAT+GGG 0.550334 5.3:+46659584 None:intergenic
ATTAAAGCTTGTTCGTGGCG+AGG 0.550376 5.3:-46659738 MS.gene74009:CDS
TTCCTATGAATAAATGGGCT+AGG 0.550588 5.3:-46659499 MS.gene74009:CDS
TCTATTGCTTCATTCTTGAA+CGG 0.552156 5.3:-46659659 MS.gene74009:CDS
CTCCATGTTGGTGCCGTGAG+TGG 0.552588 5.3:+46660240 None:intergenic
ACGAACAAGCTTTAATGAAG+AGG 0.554200 5.3:+46659745 None:intergenic
AGTTTCCTGAACAGTGGCAA+AGG 0.558363 5.3:+46659856 None:intergenic
TTCATTCTTGAACGGTGCCA+AGG 0.558389 5.3:-46659651 MS.gene74009:CDS
CATTAGTCGAGGAGGAAGTA+GGG 0.559643 5.3:-46659607 MS.gene74009:CDS
GACTTAGTGTCCTCCACTCA+CGG 0.564053 5.3:-46660253 MS.gene74009:CDS
TTGTCCAACGGAAGCAGCAA+AGG 0.565983 5.3:+46659817 None:intergenic
AGCTGTTACTCATGAAAGAA+GGG 0.566179 5.3:-46660032 MS.gene74009:CDS
TTTGCCACATCTTAGTTACC+GGG 0.572604 5.3:-46659690 MS.gene74009:CDS
GTGGACTTTGATATCATGGA+AGG 0.574432 5.3:-46660001 MS.gene74009:CDS
AAGCAATAGAATCTGAAGCC+CGG 0.574832 5.3:+46659672 None:intergenic
ATTGAATGAGTGAAGCCCAT+TGG 0.575421 5.3:+46659975 None:intergenic
CATGTCTTGAAGAAGCTGAA+AGG 0.576740 5.3:+46660105 None:intergenic
AAATGAGCAAACTTGACGTA+AGG 0.579384 5.3:+46660079 None:intergenic
TAAATGGGCTAGGAGTTTAG+TGG 0.580272 5.3:-46659489 MS.gene74009:CDS
GGACTTTGATATCATGGAAG+GGG 0.580763 5.3:-46659999 MS.gene74009:CDS
ATATCATGGAAGGGGCCCAA+TGG 0.582076 5.3:-46659991 MS.gene74009:CDS
AGAAACAAAGAACGTGGCAA+TGG 0.585187 5.3:-46659893 MS.gene74009:CDS
CTCCACTCACGGCACCAACA+TGG 0.585629 5.3:-46660242 MS.gene74009:CDS
GTGATGCGAAGATGTGGGCC+CGG 0.587438 5.3:+46659926 None:intergenic
CCACTGAGCCCATTATTCGT+GGG 0.587441 5.3:+46659450 None:intergenic
ATTTAACGATGGGTATAGGG+TGG 0.589148 5.3:-46659294 MS.gene74009:CDS
TCAATATCACTTGAGCCAGA+TGG 0.599918 5.3:+46660287 None:intergenic
CTGTTCAGGAAACTGGAAGA+AGG 0.600566 5.3:-46659847 MS.gene74009:CDS
CCATAAGGAGGTGCACTAGA+CGG 0.601550 5.3:+46660347 None:intergenic
TAGCAGAGTTTGGAGGGCGT+CGG 0.602737 5.3:+46660198 None:intergenic
GTTGTACATGACTGGTGAAG+AGG 0.605566 5.3:-46659423 MS.gene74009:CDS
AATGCAGTGATGCGAAGATG+TGG 0.609707 5.3:+46659920 None:intergenic
CATGTTGGTGCCGTGAGTGG+AGG 0.611646 5.3:+46660243 None:intergenic
TGGACTTTGATATCATGGAA+GGG 0.613948 5.3:-46660000 MS.gene74009:CDS
TCTTCCAGTTTCCTGAACAG+TGG 0.616454 5.3:+46659850 None:intergenic
CGCAGCGAGTCTCTCCATGT+TGG 0.618231 5.3:+46660228 None:intergenic
GCAAGAGAGGAGTTCTTGGG+GGG 0.619393 5.3:-46659396 MS.gene74009:CDS
AAAAGTGAACGGTTGTCCAA+CGG 0.621563 5.3:+46659805 None:intergenic
GTACATGACTGGTGAAGAGG+AGG 0.631690 5.3:-46659420 MS.gene74009:CDS
CTACATTGATGAAAAGTGAA+CGG 0.641838 5.3:+46659794 None:intergenic
TTCACCAGTCATGTACAACT+CGG 0.643539 5.3:+46659427 None:intergenic
GTGGCCGAGTTGTACATGAC+TGG 0.650298 5.3:-46659431 MS.gene74009:CDS
GAAGCCCGGTAACTAAGATG+TGG 0.661574 5.3:+46659686 None:intergenic
GAGAGTATGTCTGTTTGGTG+GGG 0.663577 5.3:+46660130 None:intergenic
GCTCGTGACATTAGTCGAGG+AGG 0.670792 5.3:-46659615 MS.gene74009:CDS
CCCACGAATAATGGGCTCAG+TGG 0.676277 5.3:-46659450 MS.gene74009:CDS
AAAGCTCGTGACATTAGTCG+AGG 0.678929 5.3:-46659618 MS.gene74009:CDS
AGCAAGAGAGGAGTTCTTGG+GGG 0.679139 5.3:-46659397 MS.gene74009:CDS
ATGCAGTGATGCGAAGATGT+GGG 0.688648 5.3:+46659921 None:intergenic
TAAGGAGGTGCACTAGACGG+AGG 0.697542 5.3:+46660350 None:intergenic
CAACTCAAATTCCACGACGG+AGG 0.699012 5.3:-46660401 MS.gene74009:CDS
TCTAGTGCACCTCCTTATGG+CGG 0.711985 5.3:-46660344 MS.gene74009:CDS
TTGTCTAGAAACAAAGAACG+TGG 0.726054 5.3:-46659899 MS.gene74009:CDS
GTCCAACTCAAATTCCACGA+CGG 0.726679 5.3:-46660404 MS.gene74009:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTGGGTTTATTTAACGAT+GGG - chr5.3:46660304-46660323 MS.gene74009:CDS 25.0%
! TTATTGGGTTTATTTAACGA+TGG - chr5.3:46660303-46660322 MS.gene74009:CDS 25.0%
! TTTATTTAACGATGGGTATA+GGG - chr5.3:46660311-46660330 MS.gene74009:CDS 25.0%
AAAGGAGTTAACATAAGCTA+TGG - chr5.3:46660258-46660277 MS.gene74009:CDS 30.0%
ATAACAACTTTGCTTGACAA+TGG + chr5.3:46660287-46660306 None:intergenic 30.0%
CTACATTGATGAAAAGTGAA+CGG + chr5.3:46659817-46659836 None:intergenic 30.0%
GTTTATTTAACGATGGGTAT+AGG - chr5.3:46660310-46660329 MS.gene74009:CDS 30.0%
TCTATTGCTTCATTCTTGAA+CGG - chr5.3:46659949-46659968 MS.gene74009:CDS 30.0%
TGGGTTTCCTATGAATAAAT+GGG - chr5.3:46660104-46660123 MS.gene74009:CDS 30.0%
TTGTCAAGCAAAGTTGTTAT+TGG - chr5.3:46660287-46660306 MS.gene74009:CDS 30.0%
! TGTCAAGCAAAGTTGTTATT+GGG - chr5.3:46660288-46660307 MS.gene74009:CDS 30.0%
! TTAGTGGAGCAAGTATTTTT+AGG - chr5.3:46660135-46660154 MS.gene74009:CDS 30.0%
AAATGAGCAAACTTGACGTA+AGG + chr5.3:46659532-46659551 None:intergenic 35.0%
AAGCTGTTACTCATGAAAGA+AGG - chr5.3:46659575-46659594 MS.gene74009:CDS 35.0%
AGAGTTGGGTAACAATAAGT+TGG - chr5.3:46660338-46660357 MS.gene74009:CDS 35.0%
AGCTGTTACTCATGAAAGAA+GGG - chr5.3:46659576-46659595 MS.gene74009:CDS 35.0%
ATTTGCCACATCTTAGTTAC+CGG - chr5.3:46659917-46659936 MS.gene74009:CDS 35.0%
CTGGGTTTCCTATGAATAAA+TGG - chr5.3:46660103-46660122 MS.gene74009:CDS 35.0%
TGGACTTTGATATCATGGAA+GGG - chr5.3:46659608-46659627 MS.gene74009:CDS 35.0%
TTGTCTAGAAACAAAGAACG+TGG - chr5.3:46659709-46659728 MS.gene74009:CDS 35.0%
TTTCATGAGTAACAGCTTCA+AGG + chr5.3:46659573-46659592 None:intergenic 35.0%
! ACGAACAAGCTTTAATGAAG+AGG + chr5.3:46659866-46659885 None:intergenic 35.0%
! CACTTTTCATCAATGTAGGT+TGG - chr5.3:46659819-46659838 MS.gene74009:CDS 35.0%
! TCTTCATTAAAGCTTGTTCG+TGG - chr5.3:46659865-46659884 MS.gene74009:CDS 35.0%
! TGGCTCAAGTGATATTGATT+CGG - chr5.3:46659326-46659345 MS.gene74009:CDS 35.0%
! TTCCTATGAATAAATGGGCT+AGG - chr5.3:46660109-46660128 MS.gene74009:CDS 35.0%
!! ACAATAAGTTGGTGTTAGGT+TGG - chr5.3:46660349-46660368 MS.gene74009:CDS 35.0%
!! ATTTTTAGGCCCACGAATAA+TGG - chr5.3:46660149-46660168 MS.gene74009:CDS 35.0%
!! CATAGTGGACTTTGATATCA+TGG - chr5.3:46659603-46659622 MS.gene74009:CDS 35.0%
!! CGTTCACTTTTCATCAATGT+AGG - chr5.3:46659815-46659834 MS.gene74009:CDS 35.0%
!! GGTAACAATAAGTTGGTGTT+AGG - chr5.3:46660345-46660364 MS.gene74009:CDS 35.0%
!! TTTTTAGGCCCACGAATAAT+GGG - chr5.3:46660150-46660169 MS.gene74009:CDS 35.0%
AAAAGTGAACGGTTGTCCAA+CGG + chr5.3:46659806-46659825 None:intergenic 40.0%
AGAAACAAAGAACGTGGCAA+TGG - chr5.3:46659715-46659734 MS.gene74009:CDS 40.0%
ATTGAATGAGTGAAGCCCAT+TGG + chr5.3:46659636-46659655 None:intergenic 40.0%
ATTTAACGATGGGTATAGGG+TGG - chr5.3:46660314-46660333 MS.gene74009:CDS 40.0%
CATGTCTTGAAGAAGCTGAA+AGG + chr5.3:46659506-46659525 None:intergenic 40.0%
CTCCTAGCCCATTTATTCAT+AGG + chr5.3:46660114-46660133 None:intergenic 40.0%
GGACTTTGATATCATGGAAG+GGG - chr5.3:46659609-46659628 MS.gene74009:CDS 40.0%
GTGGACTTTGATATCATGGA+AGG - chr5.3:46659607-46659626 MS.gene74009:CDS 40.0%
GTTTGCTTTCTGCTTCTGTT+TGG - chr5.3:46660382-46660401 MS.gene74009:CDS 40.0%
TAACAGCTTCAAGGATAGCT+TGG + chr5.3:46659564-46659583 None:intergenic 40.0%
TCAATATCACTTGAGCCAGA+TGG + chr5.3:46659324-46659343 None:intergenic 40.0%
TCACTTTCATCGCGAAAAGA+AGG - chr5.3:46659655-46659674 MS.gene74009:CDS 40.0%
TGAAAGAAGGGTTCACATAG+TGG - chr5.3:46659588-46659607 MS.gene74009:CDS 40.0%
TTCACCAGTCATGTACAACT+CGG + chr5.3:46660184-46660203 None:intergenic 40.0%
TTGAATGAGTGAAGCCCATT+GGG + chr5.3:46659635-46659654 None:intergenic 40.0%
TTTGCCACATCTTAGTTACC+GGG - chr5.3:46659918-46659937 MS.gene74009:CDS 40.0%
! AAGCAATAGAATCTGAAGCC+CGG + chr5.3:46659939-46659958 None:intergenic 40.0%
! ACTCTTGTTTGTGCCTGTTA+GGG + chr5.3:46659299-46659318 None:intergenic 40.0%
! CGTCAAGTTTGCTCATTTCA+CGG - chr5.3:46659534-46659553 MS.gene74009:CDS 40.0%
! TGAGAGTATGTCTGTTTGGT+GGG + chr5.3:46659482-46659501 None:intergenic 40.0%
!! AGTGATATTGATTCGGCTCA+AGG - chr5.3:46659333-46659352 MS.gene74009:CDS 40.0%
!! TAAATGGGCTAGGAGTTTAG+TGG - chr5.3:46660119-46660138 MS.gene74009:CDS 40.0%
AAAGCTCGTGACATTAGTCG+AGG - chr5.3:46659990-46660009 MS.gene74009:CDS 45.0%
AATGCAGTGATGCGAAGATG+TGG + chr5.3:46659691-46659710 None:intergenic 45.0%
ACAAAGCCTGCATCAGTGAT+GGG + chr5.3:46660027-46660046 None:intergenic 45.0%
ACATTAGTCGAGGAGGAAGT+AGG - chr5.3:46660000-46660019 MS.gene74009:CDS 45.0%
AGTTTCCTGAACAGTGGCAA+AGG + chr5.3:46659755-46659774 None:intergenic 45.0%
ATGCAGTGATGCGAAGATGT+GGG + chr5.3:46659690-46659709 None:intergenic 45.0%
CACAAACAAGAGTCACCATC+TGG - chr5.3:46659306-46659325 MS.gene74009:CDS 45.0%
CACAAACTCTGTCTTCTCAC+TGG - chr5.3:46659454-46659473 MS.gene74009:CDS 45.0%
CAGTGAGAAGACAGAGTTTG+TGG + chr5.3:46659456-46659475 None:intergenic 45.0%
CATTAGTCGAGGAGGAAGTA+GGG - chr5.3:46660001-46660020 MS.gene74009:CDS 45.0%
CTGTTCAGGAAACTGGAAGA+AGG - chr5.3:46659761-46659780 MS.gene74009:CDS 45.0%
GAGTTTGTGGTGACCACAAT+CGG + chr5.3:46659443-46659462 None:intergenic 45.0%
GTCCAACTCAAATTCCACGA+CGG - chr5.3:46659204-46659223 MS.gene74009:CDS 45.0%
GTTGTACATGACTGGTGAAG+AGG - chr5.3:46660185-46660204 MS.gene74009:CDS 45.0%
TCCGTTTAGCAGAGTTTGGA+GGG + chr5.3:46659419-46659438 None:intergenic 45.0%
TCTTCCAGTTTCCTGAACAG+TGG + chr5.3:46659761-46659780 None:intergenic 45.0%
TTCCGTTTAGCAGAGTTTGG+AGG + chr5.3:46659420-46659439 None:intergenic 45.0%
TTTGCCACTGTTCAGGAAAC+TGG - chr5.3:46659754-46659773 MS.gene74009:CDS 45.0%
! AGGCTGAGAGTATGTCTGTT+TGG + chr5.3:46659486-46659505 None:intergenic 45.0%
! AGGCTTTGTGGGTTTGTTCA+TGG - chr5.3:46660038-46660057 MS.gene74009:CDS 45.0%
! ATCACTGATGCAGGCTTTGT+GGG - chr5.3:46660027-46660046 MS.gene74009:CDS 45.0%
! ATTAAAGCTTGTTCGTGGCG+AGG - chr5.3:46659870-46659889 MS.gene74009:CDS 45.0%
! CTGAGAGTATGTCTGTTTGG+TGG + chr5.3:46659483-46659502 None:intergenic 45.0%
! GACTCTTGTTTGTGCCTGTT+AGG + chr5.3:46659300-46659319 None:intergenic 45.0%
! GAGAGTATGTCTGTTTGGTG+GGG + chr5.3:46659481-46659500 None:intergenic 45.0%
! TTCATTCTTGAACGGTGCCA+AGG - chr5.3:46659957-46659976 MS.gene74009:CDS 45.0%
!! GAGCTTTGTTCCTAACTCCT+TGG + chr5.3:46659977-46659996 None:intergenic 45.0%
AGCAAGAGAGGAGTTCTTGG+GGG - chr5.3:46660211-46660230 MS.gene74009:CDS 50.0%
AGCGATGTATGACTCGTTCG+AGG - chr5.3:46660080-46660099 MS.gene74009:CDS 50.0%
AGGAGCAAGAGAGGAGTTCT+TGG - chr5.3:46660208-46660227 MS.gene74009:CDS 50.0%
ATATCATGGAAGGGGCCCAA+TGG - chr5.3:46659617-46659636 MS.gene74009:CDS 50.0%
ATCGTCCTTTGCCACTGTTC+AGG - chr5.3:46659747-46659766 MS.gene74009:CDS 50.0%
ATGTATGACTCGTTCGAGGC+TGG - chr5.3:46660084-46660103 MS.gene74009:CDS 50.0%
CAACTCAAATTCCACGACGG+AGG - chr5.3:46659207-46659226 MS.gene74009:CDS 50.0%
CACAAAGCCTGCATCAGTGA+TGG + chr5.3:46660028-46660047 None:intergenic 50.0%
CATCACTGATGCAGGCTTTG+TGG - chr5.3:46660026-46660045 MS.gene74009:CDS 50.0%
CCACTGAGCCCATTATTCGT+GGG + chr5.3:46660161-46660180 None:intergenic 50.0%
CGGTTCCGTTTAGCAGAGTT+TGG + chr5.3:46659423-46659442 None:intergenic 50.0%
CTCCGTCGTGGAATTTGAGT+TGG + chr5.3:46659209-46659228 None:intergenic 50.0%
CTGCTAAACGGAACCGATTG+TGG - chr5.3:46659427-46659446 MS.gene74009:CDS 50.0%
GAAGCCCGGTAACTAAGATG+TGG + chr5.3:46659925-46659944 None:intergenic 50.0%
GACTTAGTGTCCTCCACTCA+CGG - chr5.3:46659355-46659374 MS.gene74009:CDS 50.0%
GAGCAAGAGAGGAGTTCTTG+GGG - chr5.3:46660210-46660229 MS.gene74009:CDS 50.0%
GAGGAGGAGGATGACAGAAA+GGG - chr5.3:46659235-46659254 MS.gene74009:CDS 50.0%
GCCCTCCAAACTCTGCTAAA+CGG - chr5.3:46659415-46659434 MS.gene74009:CDS 50.0%
GGAGCAAGAGAGGAGTTCTT+GGG - chr5.3:46660209-46660228 MS.gene74009:CDS 50.0%
GGGTATAGGGTGGAAGAGTT+GGG - chr5.3:46660324-46660343 MS.gene74009:CDS 50.0%
GTACATGACTGGTGAAGAGG+AGG - chr5.3:46660188-46660207 MS.gene74009:CDS 50.0%
TATCATGGAAGGGGCCCAAT+GGG - chr5.3:46659618-46659637 MS.gene74009:CDS 50.0%
TCTAGTGCACCTCCTTATGG+CGG - chr5.3:46659264-46659283 MS.gene74009:CDS 50.0%
TGGGTATAGGGTGGAAGAGT+TGG - chr5.3:46660323-46660342 MS.gene74009:CDS 50.0%
TGTATGACTCGTTCGAGGCT+GGG - chr5.3:46660085-46660104 MS.gene74009:CDS 50.0%
TTGGGTGTGGAAGGGTTCAA+AGG - chr5.3:46660240-46660259 MS.gene74009:CDS 50.0%
TTGTCCAACGGAAGCAGCAA+AGG + chr5.3:46659794-46659813 None:intergenic 50.0%
! ACAACCTTTGCTGCTTCCGT+TGG - chr5.3:46659787-46659806 MS.gene74009:CDS 50.0%
! CCATAAGGAGGTGCACTAGA+CGG + chr5.3:46659264-46659283 None:intergenic 50.0%
! TTGAACGGTGCCAAGGAGTT+AGG - chr5.3:46659964-46659983 MS.gene74009:CDS 50.0%
AAATTCCACGACGGAGGAGG+AGG - chr5.3:46659213-46659232 MS.gene74009:CDS 55.0%
AGTTCTTGGGGGGAGTGGTT+GGG - chr5.3:46660222-46660241 MS.gene74009:CDS 55.0%
ATCGCGAAAAGAAGGCCTGC+CGG - chr5.3:46659663-46659682 MS.gene74009:CDS 55.0%
CCGTCTAGTGCACCTCCTTA+TGG - chr5.3:46659261-46659280 MS.gene74009:CDS 55.0%
CTCAAATTCCACGACGGAGG+AGG - chr5.3:46659210-46659229 MS.gene74009:CDS 55.0%
GCAAGAGAGGAGTTCTTGGG+GGG - chr5.3:46660212-46660231 MS.gene74009:CDS 55.0%
GCCACTGAGCCCATTATTCG+TGG + chr5.3:46660162-46660181 None:intergenic 55.0%
GCTCGTGACATTAGTCGAGG+AGG - chr5.3:46659993-46660012 MS.gene74009:CDS 55.0%
GGAGGAGGAGGATGACAGAA+AGG - chr5.3:46659234-46659253 MS.gene74009:CDS 55.0%
GTGAAGAGGAGGAGCAAGAG+AGG - chr5.3:46660199-46660218 MS.gene74009:CDS 55.0%
GTGGCCGAGTTGTACATGAC+TGG - chr5.3:46660177-46660196 MS.gene74009:CDS 55.0%
! TAAGGAGGTGCACTAGACGG+AGG + chr5.3:46659261-46659280 None:intergenic 55.0%
! TAGCAGAGTTTGGAGGGCGT+CGG + chr5.3:46659413-46659432 None:intergenic 55.0%
!! CCCACGAATAATGGGCTCAG+TGG - chr5.3:46660158-46660177 MS.gene74009:CDS 55.0%
AGAGGAGTTCTTGGGGGGAG+TGG - chr5.3:46660217-46660236 MS.gene74009:CDS 60.0%
CGCAGCGAGTCTCTCCATGT+TGG + chr5.3:46659383-46659402 None:intergenic 60.0%
CTCCACTCACGGCACCAACA+TGG - chr5.3:46659366-46659385 MS.gene74009:CDS 60.0%
GAGTTCTTGGGGGGAGTGGT+TGG - chr5.3:46660221-46660240 MS.gene74009:CDS 60.0%
GGGAGTGGTTGGGTGTGGAA+GGG - chr5.3:46660232-46660251 MS.gene74009:CDS 60.0%
GTAGGGCCCATCACTGATGC+AGG - chr5.3:46660018-46660037 MS.gene74009:CDS 60.0%
GTGATGCGAAGATGTGGGCC+CGG + chr5.3:46659685-46659704 None:intergenic 60.0%
TCGCGAAAAGAAGGCCTGCC+GGG - chr5.3:46659664-46659683 MS.gene74009:CDS 60.0%
!! CATGTTGGTGCCGTGAGTGG+AGG + chr5.3:46659368-46659387 None:intergenic 60.0%
!! CTCCATGTTGGTGCCGTGAG+TGG + chr5.3:46659371-46659390 None:intergenic 60.0%
GCACCTCCTTATGGCGGCAG+CGG - chr5.3:46659270-46659289 MS.gene74009:CDS 65.0%
GGGGAGTGGTTGGGTGTGGA+AGG - chr5.3:46660231-46660250 MS.gene74009:CDS 65.0%
TTCCACGACGGAGGAGGAGG+AGG - chr5.3:46659216-46659235 MS.gene74009:CDS 65.0%
TTGGGGGGAGTGGTTGGGTG+TGG - chr5.3:46660227-46660246 MS.gene74009:CDS 65.0%
CACGACGGAGGAGGAGGAGG+AGG - chr5.3:46659219-46659238 MS.gene74009:CDS 70.0%
CCTCCGCTGCCGCCATAAGG+AGG + chr5.3:46659276-46659295 None:intergenic 70.0%
CCTCCTTATGGCGGCAGCGG+AGG - chr5.3:46659273-46659292 MS.gene74009:CDS 70.0%
CTCCTCCTCCTCCTCCGTCG+TGG + chr5.3:46659221-46659240 None:intergenic 70.0%
GACGGAGGAGGAGGAGGAGG+AGG - chr5.3:46659222-46659241 MS.gene74009:CDS 70.0%
TGCGAAGATGTGGGCCCGGC+AGG + chr5.3:46659681-46659700 None:intergenic 70.0%
GCGGCAGCGGAGGCCCTAAC+AGG - chr5.3:46659283-46659302 MS.gene74009:CDS 75.0%
GGGCCTCCGCTGCCGCCATA+AGG + chr5.3:46659279-46659298 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 46659193 46660437 46659193 ID=MS.gene74009
chr5.3 mRNA 46659193 46660437 46659193 ID=MS.gene74009.t1;Parent=MS.gene74009
chr5.3 exon 46659193 46660437 46659193 ID=MS.gene74009.t1.exon1;Parent=MS.gene74009.t1
chr5.3 CDS 46659193 46660437 46659193 ID=cds.MS.gene74009.t1;Parent=MS.gene74009.t1
Gene Sequence

>MS.gene74009

ATGGGAGACATGTCCAACTCAAATTCCACGACGGAGGAGGAGGAGGAGGAGGATGACAGAAAGGGCCTCCGTCTAGTGCACCTCCTTATGGCGGCAGCGGAGGCCCTAACAGGCACAAACAAGAGTCACCATCTGGCTCAAGTGATATTGATTCGGCTCAAGGACTTAGTGTCCTCCACTCACGGCACCAACATGGAGAGACTCGCTGCGTATTTTACCGACGCCCTCCAAACTCTGCTAAACGGAACCGATTGTGGTCACCACAAACTCTGTCTTCTCACTGGACCCCACCAAACAGACATACTCTCAGCCTTTCAGCTTCTTCAAGACATGTCTCCTTACGTCAAGTTTGCTCATTTCACGGCAAACCAAGCTATCCTTGAAGCTGTTACTCATGAAAGAAGGGTTCACATAGTGGACTTTGATATCATGGAAGGGGCCCAATGGGCTTCACTCATTCAATCACTTTCATCGCGAAAAGAAGGCCTGCCGGGCCCACATCTTCGCATCACTGCATTGTCTAGAAACAAAGAACGTGGCAATGGACGATCTAGATCGTCCTTTGCCACTGTTCAGGAAACTGGAAGAAGGTTAACAACCTTTGCTGCTTCCGTTGGACAACCGTTCACTTTTCATCAATGTAGGTTGGACTCAGACGAAACGTTTCGAACCTCTTCATTAAAGCTTGTTCGTGGCGAGGCTTTAGTTTTTAACTGTGTCATGCATTTGCCACATCTTAGTTACCGGGCTTCAGATTCTATTGCTTCATTCTTGAACGGTGCCAAGGAGTTAGGAACAAAGCTCGTGACATTAGTCGAGGAGGAAGTAGGGCCCATCACTGATGCAGGCTTTGTGGGTTTGTTCATGGATTCATTGCATCGTTATTCAGCGATGTATGACTCGTTCGAGGCTGGGTTTCCTATGAATAAATGGGCTAGGAGTTTAGTGGAGCAAGTATTTTTAGGCCCACGAATAATGGGCTCAGTGGCCGAGTTGTACATGACTGGTGAAGAGGAGGAGCAAGAGAGGAGTTCTTGGGGGGAGTGGTTGGGTGTGGAAGGGTTCAAAGGAGTTAACATAAGCTATGGTAACCATTGTCAAGCAAAGTTGTTATTGGGTTTATTTAACGATGGGTATAGGGTGGAAGAGTTGGGTAACAATAAGTTGGTGTTAGGTTGGAAATCAAGACGTTTGCTTTCTGCTTCTGTTTGGACTTGTTTGTCAGAGATTGAATATGATTTTTAA
Protein sequence

>MS.gene74009.t1

MGDMSNSNSTTEEEEEEDDRKGLRLVHLLMAAAEALTGTNKSHHLAQVILIRLKDLVSSTHGTNMERLAAYFTDALQTLLNGTDCGHHKLCLLTGPHQTDILSAFQLLQDMSPYVKFAHFTANQAILEAVTHERRVHIVDFDIMEGAQWASLIQSLSSRKEGLPGPHLRITALSRNKERGNGRSRSSFATVQETGRRLTTFAASVGQPFTFHQCRLDSDETFRTSSLKLVRGEALVFNCVMHLPHLSYRASDSIASFLNGAKELGTKLVTLVEEEVGPITDAGFVGLFMDSLHRYSAMYDSFEAGFPMNKWARSLVEQVFLGPRIMGSVAELYMTGEEEEQERSSWGEWLGVEGFKGVNISYGNHCQAKLLLGLFNDGYRVEELGNNKLVLGWKSRRLLSASVWTCLSEIEYDF