Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74043.t1 | AES78040.1 | 96.2 | 183 | 7 | 0 | 1 | 183 | 1 | 183 | 4.10E-89 | 337.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74043.t1 | Q5QMM3 | 46.9 | 179 | 68 | 4 | 1 | 152 | 1 | 179 | 3.3e-31 | 136.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74043.t1 | G7KVS3 | 96.2 | 183 | 7 | 0 | 1 | 183 | 1 | 183 | 2.9e-89 | 337.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene74043.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74043.t1 | MTR_7g023810 | 96.175 | 183 | 7 | 0 | 1 | 183 | 1 | 183 | 5.15e-131 | 365 |
| MS.gene74043.t1 | MTR_1g115315 | 60.215 | 186 | 52 | 5 | 1 | 165 | 5 | 189 | 1.59e-66 | 204 |
| MS.gene74043.t1 | MTR_1g115315 | 60.215 | 186 | 52 | 5 | 1 | 165 | 5 | 189 | 1.62e-66 | 204 |
| MS.gene74043.t1 | MTR_3g115620 | 44.503 | 191 | 80 | 4 | 9 | 176 | 7 | 194 | 4.18e-45 | 148 |
| MS.gene74043.t1 | MTR_4g084550 | 33.071 | 127 | 73 | 3 | 36 | 150 | 25 | 151 | 1.43e-13 | 65.9 |
| MS.gene74043.t1 | MTR_8g107210 | 48.387 | 62 | 32 | 0 | 68 | 129 | 96 | 157 | 5.47e-13 | 66.2 |
| MS.gene74043.t1 | MTR_2g015000 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 1.67e-11 | 62.4 |
| MS.gene74043.t1 | MTR_4g088080 | 43.333 | 60 | 34 | 0 | 66 | 125 | 38 | 97 | 5.17e-11 | 60.5 |
| MS.gene74043.t1 | MTR_4g063735 | 45.161 | 62 | 34 | 0 | 68 | 129 | 25 | 86 | 9.56e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74043.t1 | AT4G35550 | 56.579 | 152 | 46 | 2 | 17 | 148 | 33 | 184 | 1.41e-46 | 154 |
| MS.gene74043.t1 | AT1G20700 | 46.667 | 150 | 57 | 4 | 15 | 141 | 24 | 173 | 1.38e-35 | 124 |
| MS.gene74043.t1 | AT1G20700 | 46.667 | 150 | 57 | 4 | 15 | 141 | 24 | 173 | 1.72e-35 | 124 |
| MS.gene74043.t1 | AT1G20710 | 47.244 | 127 | 47 | 2 | 21 | 128 | 14 | 139 | 6.80e-26 | 98.6 |
| MS.gene74043.t1 | AT1G20700 | 54.321 | 81 | 32 | 1 | 66 | 141 | 31 | 111 | 7.09e-23 | 89.7 |
| MS.gene74043.t1 | AT3G18010 | 37.500 | 104 | 58 | 3 | 68 | 164 | 76 | 179 | 4.80e-12 | 63.5 |
| MS.gene74043.t1 | AT2G33880 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 2.32e-11 | 61.6 |
| MS.gene74043.t1 | AT2G33880 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 2.45e-11 | 61.6 |
| MS.gene74043.t1 | AT5G45980 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 6.21e-11 | 60.5 |
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGACCAGTGTGTATAAT+TGG | 0.216618 | 7.4:+77340135 | MS.gene74043:CDS |
| TACTTGCATCATTCCAATAT+TGG | 0.240745 | 7.4:-77339658 | None:intergenic |
| TTTAATCAGAACGCCAATAT+TGG | 0.268262 | 7.4:+77339645 | MS.gene74043:CDS |
| ACCTGTGCAACTGCAAAGTT+TGG | 0.336692 | 7.4:+77340025 | MS.gene74043:CDS |
| TGTATAATTGGTTTCAGAAT+AGG | 0.361284 | 7.4:+77340147 | MS.gene74043:CDS |
| GAGATCTTGTTGAGAAGATA+AGG | 0.380614 | 7.4:-77339772 | None:intergenic |
| TCCTAGTAAGGAAAAGATAA+AGG | 0.397905 | 7.4:+77340076 | MS.gene74043:CDS |
| TACACACTGGTCTCTGAAAT+CGG | 0.399480 | 7.4:-77340128 | None:intergenic |
| TTTGTGCAGCCAGTACTTAG+AGG | 0.411680 | 7.4:+77340292 | MS.gene74043:CDS |
| CTCTGAAATCGGCCCGTGCT+TGG | 0.426359 | 7.4:-77340117 | None:intergenic |
| TCAACCTCAGTCTCTACTTC+CGG | 0.438371 | 7.4:-77340215 | None:intergenic |
| AAATAATGTTAATGATGAAC+CGG | 0.459463 | 7.4:+77340196 | MS.gene74043:CDS |
| GACTGAGATGCAACGATCTC+CGG | 0.461176 | 7.4:-77340260 | None:intergenic |
| CGAATATTTGAAGCTGAGAC+GGG | 0.462716 | 7.4:+77340050 | MS.gene74043:CDS |
| ATGAACAAGTCGAAACTCTT+CGG | 0.463263 | 7.4:+77339694 | MS.gene74043:CDS |
| TCGTAAACTGTCATCTGATT+CGG | 0.469903 | 7.4:-77340324 | None:intergenic |
| TCCAAACTTTGCAGTTGCAC+AGG | 0.484501 | 7.4:-77340026 | None:intergenic |
| CTACTAGGCTGAGGCAGCGT+TGG | 0.489931 | 7.4:+77339994 | MS.gene74043:CDS |
| CAATGATAAGAAAACTGAAC+CGG | 0.495461 | 7.4:+77340241 | MS.gene74043:CDS |
| AAGAGTTTCGACTTGTTCAT+CGG | 0.503160 | 7.4:-77339691 | None:intergenic |
| GGTTTCAGAATAGGCGTGCT+AGG | 0.518890 | 7.4:+77340156 | MS.gene74043:CDS |
| AATTCAAGTCCTCTAAGTAC+TGG | 0.519200 | 7.4:-77340301 | None:intergenic |
| GCGAATATTTGAAGCTGAGA+CGG | 0.525730 | 7.4:+77340049 | MS.gene74043:CDS |
| TGAGACGGGGACTCCTAGTA+AGG | 0.526200 | 7.4:+77340064 | MS.gene74043:CDS |
| CTTTGCAGTTGCACAGGTGT+TGG | 0.529341 | 7.4:-77340020 | None:intergenic |
| GAATAGGCGTGCTAGGTCAA+AGG | 0.534265 | 7.4:+77340163 | MS.gene74043:CDS |
| AATAGGCGTGCTAGGTCAAA+GGG | 0.534387 | 7.4:+77340164 | MS.gene74043:CDS |
| CAACAAGATCTCACTCTCAC+TGG | 0.535564 | 7.4:+77339783 | MS.gene74043:CDS |
| ACCGCTGATTTAACCAAGCA+CGG | 0.547494 | 7.4:+77340104 | MS.gene74043:CDS |
| CCCGTGCTTGGTTAAATCAG+CGG | 0.597671 | 7.4:-77340105 | None:intergenic |
| GAGATGGAGTGGCAGAAGAA+CGG | 0.607848 | 7.4:+77339621 | None:intergenic |
| CCGCTGATTTAACCAAGCAC+GGG | 0.638814 | 7.4:+77340105 | MS.gene74043:CDS |
| CTGAAACCAATTATACACAC+TGG | 0.642488 | 7.4:-77340141 | None:intergenic |
| GAATATTTGAAGCTGAGACG+GGG | 0.658394 | 7.4:+77340051 | MS.gene74043:CDS |
| TGAACCGGAAGTAGAGACTG+AGG | 0.698771 | 7.4:+77340211 | MS.gene74043:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTAATGATGATTTTT+AGG | + | chr7.4:77339969-77339988 | MS.gene74043:intron | 15.0% |
| !!! | ACATTTAATGATGATTTTTA+GGG | + | chr7.4:77339970-77339989 | MS.gene74043:intron | 15.0% |
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr7.4:77340196-77340215 | MS.gene74043:CDS | 20.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr7.4:77340147-77340166 | MS.gene74043:CDS | 25.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr7.4:77340241-77340260 | MS.gene74043:CDS | 30.0% | |
| TACTTGCATCATTCCAATAT+TGG | - | chr7.4:77339661-77339680 | None:intergenic | 30.0% | |
| TCCTAGTAAGGAAAAGATAA+AGG | + | chr7.4:77340076-77340095 | MS.gene74043:CDS | 30.0% | |
| TTTAATCAGAACGCCAATAT+TGG | + | chr7.4:77339645-77339664 | MS.gene74043:CDS | 30.0% | |
| ! | TCCTTTATCTTTTCCTTACT+AGG | - | chr7.4:77340080-77340099 | None:intergenic | 30.0% |
| AATTCAAGTCCTCTAAGTAC+TGG | - | chr7.4:77340304-77340323 | None:intergenic | 35.0% | |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr7.4:77339694-77339713 | MS.gene74043:CDS | 35.0% | |
| CTGAAACCAATTATACACAC+TGG | - | chr7.4:77340144-77340163 | None:intergenic | 35.0% | |
| CTTCAGTTACTCATTCTCAA+CGG | + | chr7.4:77339827-77339846 | MS.gene74043:intron | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr7.4:77339775-77339794 | None:intergenic | 35.0% | |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr7.4:77339884-77339903 | MS.gene74043:intron | 35.0% | |
| TCGTAAACTGTCATCTGATT+CGG | - | chr7.4:77340327-77340346 | None:intergenic | 35.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr7.4:77339694-77339713 | None:intergenic | 35.0% |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr7.4:77339876-77339895 | None:intergenic | 35.0% |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr7.4:77340243-77340262 | None:intergenic | 35.0% |
| !!! | GATGATTTTTAGGGTCTACT+AGG | + | chr7.4:77339979-77339998 | MS.gene74043:intron | 35.0% |
| AACCAAACCGTACTAAAGAC+CGG | - | chr7.4:77339894-77339913 | None:intergenic | 40.0% | |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr7.4:77340135-77340154 | MS.gene74043:CDS | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr7.4:77339872-77339891 | MS.gene74043:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr7.4:77339875-77339894 | None:intergenic | 40.0% | |
| TCAACGGTATCACAGTTAAC+CGG | + | chr7.4:77339843-77339862 | MS.gene74043:intron | 40.0% | |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr7.4:77340050-77340069 | MS.gene74043:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr7.4:77340051-77340070 | MS.gene74043:CDS | 40.0% |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr7.4:77340049-77340068 | MS.gene74043:CDS | 40.0% |
| !! | TACACACTGGTCTCTGAAAT+CGG | - | chr7.4:77340131-77340150 | None:intergenic | 40.0% |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr7.4:77339889-77339908 | MS.gene74043:intron | 45.0% | |
| AATAGGCGTGCTAGGTCAAA+GGG | + | chr7.4:77340164-77340183 | MS.gene74043:CDS | 45.0% | |
| ACCTGTGCAACTGCAAAGTT+TGG | + | chr7.4:77340025-77340044 | MS.gene74043:CDS | 45.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr7.4:77339783-77339802 | MS.gene74043:CDS | 45.0% | |
| CAACGGTATCACAGTTAACC+GGG | + | chr7.4:77339844-77339863 | MS.gene74043:intron | 45.0% | |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr7.4:77340218-77340237 | None:intergenic | 45.0% | |
| TCCAAACTTTGCAGTTGCAC+AGG | - | chr7.4:77340029-77340048 | None:intergenic | 45.0% | |
| TTTGTGCAGCCAGTACTTAG+AGG | + | chr7.4:77340292-77340311 | MS.gene74043:CDS | 45.0% | |
| !! | ACCGCTGATTTAACCAAGCA+CGG | + | chr7.4:77340104-77340123 | MS.gene74043:CDS | 45.0% |
| !!! | TTTTAGGGTCTACTAGGCTG+AGG | + | chr7.4:77339985-77340004 | MS.gene74043:intron | 45.0% |
| CCCGTGCTTGGTTAAATCAG+CGG | - | chr7.4:77340108-77340127 | None:intergenic | 50.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr7.4:77340023-77340042 | None:intergenic | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr7.4:77340163-77340182 | MS.gene74043:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr7.4:77340263-77340282 | None:intergenic | 50.0% | |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr7.4:77340156-77340175 | MS.gene74043:CDS | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr7.4:77340211-77340230 | MS.gene74043:CDS | 50.0% | |
| !! | CCGCTGATTTAACCAAGCAC+GGG | + | chr7.4:77340105-77340124 | MS.gene74043:CDS | 50.0% |
| ATATTGAACCGGGCTCCACC+CGG | - | chr7.4:77339865-77339884 | None:intergenic | 55.0% | |
| CGGTATCACAGTTAACCGGG+TGG | + | chr7.4:77339847-77339866 | MS.gene74043:intron | 55.0% | |
| TGAGACGGGGACTCCTAGTA+AGG | + | chr7.4:77340064-77340083 | MS.gene74043:CDS | 55.0% | |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr7.4:77339854-77339873 | MS.gene74043:intron | 60.0% | |
| CTACTAGGCTGAGGCAGCGT+TGG | + | chr7.4:77339994-77340013 | MS.gene74043:CDS | 60.0% | |
| !! | CTCTGAAATCGGCCCGTGCT+TGG | - | chr7.4:77340120-77340139 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 77339624 | 77340361 | 77339624 | ID=MS.gene74043 |
| chr7.4 | mRNA | 77339624 | 77340361 | 77339624 | ID=MS.gene74043.t1;Parent=MS.gene74043 |
| chr7.4 | exon | 77339624 | 77339804 | 77339624 | ID=MS.gene74043.t1.exon1;Parent=MS.gene74043.t1 |
| chr7.4 | CDS | 77339624 | 77339804 | 77339624 | ID=cds.MS.gene74043.t1;Parent=MS.gene74043.t1 |
| chr7.4 | exon | 77339991 | 77340361 | 77339991 | ID=MS.gene74043.t1.exon2;Parent=MS.gene74043.t1 |
| chr7.4 | CDS | 77339991 | 77340361 | 77339991 | ID=cds.MS.gene74043.t1;Parent=MS.gene74043.t1 |
| Gene Sequence |
| Protein sequence |
