Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74206.t1 | XP_003592777.1 | 94.5 | 311 | 17 | 0 | 46 | 356 | 141 | 451 | 2.00E-164 | 588.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74206.t1 | B7ZUF3 | 25.8 | 178 | 120 | 3 | 46 | 212 | 187 | 363 | 2.0e-08 | 61.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74206.t1 | G7IF12 | 94.5 | 311 | 17 | 0 | 46 | 356 | 141 | 451 | 1.4e-164 | 588.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene74206.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74206.t1 | MTR_1g115980 | 94.534 | 311 | 17 | 0 | 46 | 356 | 141 | 451 | 0.0 | 589 |
| MS.gene74206.t1 | MTR_1g115980 | 92.283 | 311 | 15 | 1 | 46 | 356 | 141 | 442 | 0.0 | 568 |
| MS.gene74206.t1 | MTR_1g115980 | 94.677 | 263 | 14 | 0 | 46 | 308 | 141 | 403 | 0.0 | 519 |
| MS.gene74206.t1 | MTR_1g115980 | 92.015 | 263 | 12 | 1 | 46 | 308 | 141 | 394 | 7.62e-177 | 497 |
| MS.gene74206.t1 | MTR_1g115980 | 93.636 | 220 | 14 | 0 | 46 | 265 | 141 | 360 | 1.12e-152 | 434 |
| MS.gene74206.t1 | MTR_1g115980 | 90.455 | 220 | 12 | 1 | 46 | 265 | 141 | 351 | 3.05e-144 | 412 |
| MS.gene74206.t1 | MTR_3g082690 | 25.387 | 323 | 205 | 12 | 33 | 341 | 164 | 464 | 5.84e-13 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74206.t1 | AT3G55080 | 57.235 | 311 | 130 | 2 | 46 | 355 | 35 | 343 | 4.67e-123 | 358 |
| MS.gene74206.t1 | AT3G55080 | 57.235 | 311 | 130 | 2 | 46 | 355 | 66 | 374 | 1.47e-122 | 358 |
| MS.gene74206.t1 | AT3G55080 | 57.235 | 311 | 130 | 2 | 46 | 355 | 66 | 374 | 1.47e-122 | 358 |
| MS.gene74206.t1 | AT3G55080 | 57.235 | 311 | 130 | 2 | 46 | 355 | 150 | 458 | 1.78e-121 | 358 |
| MS.gene74206.t1 | AT3G55080 | 56.329 | 316 | 130 | 3 | 46 | 355 | 35 | 348 | 1.67e-120 | 352 |
| MS.gene74206.t1 | AT3G07670 | 28.342 | 187 | 120 | 8 | 33 | 209 | 171 | 353 | 2.82e-12 | 68.2 |
| MS.gene74206.t1 | AT3G07670 | 28.342 | 187 | 120 | 8 | 33 | 209 | 33 | 215 | 3.13e-12 | 67.4 |
Find 77 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATGCTCCGCAATTAAT+TGG | 0.158521 | 1.2:-86089007 | None:intergenic |
| CTCCGAGCCCTTTGCATCTT+TGG | 0.188607 | 1.2:-86087997 | None:intergenic |
| TCTTTCTTGTACCACTCTTC+AGG | 0.243444 | 1.2:+86088722 | MS.gene74206:CDS |
| CCGTCAAAGTTCAGTTTATC+AGG | 0.269055 | 1.2:+86078332 | MS.gene74206:CDS |
| TCCTCTGCGTCACACAAAAT+TGG | 0.272658 | 1.2:+86087944 | MS.gene74206:CDS |
| TTTCAGGTGCTTATAAGATA+TGG | 0.280416 | 1.2:+86087676 | MS.gene74206:intron |
| GCTGGCTAGGCGCCCTTTAC+AGG | 0.304269 | 1.2:+86088935 | MS.gene74206:CDS |
| TATTATGCATCTCGGCTTGC+GGG | 0.336854 | 1.2:-86078134 | None:intergenic |
| GGAGCATAATGCAGCTATTA+TGG | 0.347092 | 1.2:+86089019 | MS.gene74206:CDS |
| GGGAAGCGTACCACAGAATA+AGG | 0.348533 | 1.2:-86077803 | None:intergenic |
| AATCACTTCGTGCACTTGCT+CGG | 0.349523 | 1.2:+86088697 | MS.gene74206:CDS |
| CCTGATAAACTGAACTTTGA+CGG | 0.359051 | 1.2:-86078332 | None:intergenic |
| TCTGATCGTGATTATGTTCC+TGG | 0.368828 | 1.2:+86081624 | MS.gene74206:CDS |
| CGAGCCATTCGTCTTCTAAC+AGG | 0.372330 | 1.2:-86089322 | None:intergenic |
| CTTTGTTCATATCCTGTAAA+GGG | 0.388424 | 1.2:-86088947 | None:intergenic |
| CCCTTTGCATCTTTGGTTGG+AGG | 0.393470 | 1.2:-86087990 | None:intergenic |
| GTGAAGTCTGCTGGAGGAAA+AGG | 0.398194 | 1.2:+86088660 | MS.gene74206:CDS |
| CTTTCTTGTACCACTCTTCA+GGG | 0.401046 | 1.2:+86088723 | MS.gene74206:CDS |
| GAGCCCTTTGCATCTTTGGT+TGG | 0.427651 | 1.2:-86087993 | None:intergenic |
| ATCACTTCGTGCACTTGCTC+GGG | 0.429403 | 1.2:+86088698 | MS.gene74206:CDS |
| ACCTGATCATAAATGTTGTA+TGG | 0.431705 | 1.2:-86087745 | None:intergenic |
| TCCATACAACATTTATGATC+AGG | 0.435626 | 1.2:+86087744 | MS.gene74206:CDS |
| AATGCCAGAGCTTAACCATT+TGG | 0.439954 | 1.2:+86088884 | MS.gene74206:intron |
| TGATTATGTTCCTGGTGAAC+AGG | 0.442198 | 1.2:+86081632 | MS.gene74206:CDS |
| CATAGGGAGGTGAAGTCTGC+TGG | 0.442386 | 1.2:+86088651 | MS.gene74206:intron |
| TCTTTGTTCATATCCTGTAA+AGG | 0.450034 | 1.2:-86088948 | None:intergenic |
| TTCAAATGCTACATTGATGC+TGG | 0.452747 | 1.2:+86087705 | MS.gene74206:CDS |
| CCAAAAGTCTCAAATTGAAA+AGG | 0.459340 | 1.2:+86078365 | MS.gene74206:CDS |
| GCTACATTGATGCTGGACTT+TGG | 0.464448 | 1.2:+86087712 | MS.gene74206:CDS |
| CAAATCTGCTTCTACCTGGC+TGG | 0.469867 | 1.2:+86089375 | MS.gene74206:CDS |
| AAACTGATGGTAGGCTGGCT+AGG | 0.476607 | 1.2:+86088922 | MS.gene74206:CDS |
| CAGATTTCTTGAATCATGAC+GGG | 0.480634 | 1.2:+86081026 | MS.gene74206:CDS |
| GCTTCTAAATCAATACAAAC+CGG | 0.487852 | 1.2:+86077761 | MS.gene74206:CDS |
| AACCAAAGATGCAAAGGGCT+CGG | 0.490231 | 1.2:+86087995 | MS.gene74206:CDS |
| GTGCAGCCTCCGTGACCAAA+TGG | 0.495854 | 1.2:-86088899 | None:intergenic |
| CATACTTAGGTATATCGTAC+TGG | 0.499200 | 1.2:-86087920 | None:intergenic |
| GACCAGTTGGCTCTAGAGCA+TGG | 0.502799 | 1.2:+86080894 | MS.gene74206:intron |
| AAGCACAAAGGGTTTATCTC+TGG | 0.505327 | 1.2:+86080919 | MS.gene74206:CDS |
| TGCACAAACTGATGGTAGGC+TGG | 0.508432 | 1.2:+86088917 | MS.gene74206:CDS |
| ACCAGTTGGCTCTAGAGCAT+GGG | 0.517262 | 1.2:+86080895 | MS.gene74206:intron |
| GCAAGCCGAGATGCATAATA+CGG | 0.530146 | 1.2:+86078137 | MS.gene74206:CDS |
| GTATTATGCATCTCGGCTTG+CGG | 0.530651 | 1.2:-86078135 | None:intergenic |
| AGAGCATGGGGAAGCACAAA+GGG | 0.532315 | 1.2:+86080908 | MS.gene74206:CDS |
| TTCTCAAATCTGCTTCTACC+TGG | 0.536097 | 1.2:+86089371 | MS.gene74206:CDS |
| TGAAGTCTGCTGGAGGAAAA+GGG | 0.538297 | 1.2:+86088661 | MS.gene74206:CDS |
| GCCTCCAACCAAAGATGCAA+AGG | 0.545403 | 1.2:+86087989 | MS.gene74206:CDS |
| ACTTGCCGTATTATGCATCT+CGG | 0.546722 | 1.2:-86078142 | None:intergenic |
| TTCATGCATGCATGCGCACT+AGG | 0.547297 | 1.2:+86079320 | MS.gene74206:CDS |
| AACTTGTAAAATGCAATCAC+CGG | 0.549061 | 1.2:-86077780 | None:intergenic |
| TAGTATTTGACCTGTTCACC+AGG | 0.550072 | 1.2:-86081642 | None:intergenic |
| GCAGATTTCTTGAATCATGA+CGG | 0.555755 | 1.2:+86081025 | MS.gene74206:CDS |
| TTGTATGACAGGACTCTGAA+TGG | 0.556237 | 1.2:+86078088 | MS.gene74206:intron |
| AATTAATTGGATGAATAATG+AGG | 0.556334 | 1.2:-86088994 | None:intergenic |
| GCAAGTGCACGAAGTGATTG+TGG | 0.565446 | 1.2:-86088693 | None:intergenic |
| ATTATTCATCCAATTAATTG+CGG | 0.565451 | 1.2:+86088998 | MS.gene74206:CDS |
| TTTACAAGTTCCTTATTCTG+TGG | 0.566855 | 1.2:+86077793 | MS.gene74206:CDS |
| CCTCCAACCAAAGATGCAAA+GGG | 0.571025 | 1.2:+86087990 | MS.gene74206:CDS |
| AACAGGAAGTCTTTCACTCG+AGG | 0.574466 | 1.2:-86089305 | None:intergenic |
| ATTATGCATCTCGGCTTGCG+GGG | 0.583027 | 1.2:-86078133 | None:intergenic |
| TGACGCAGAGGATCATACTT+AGG | 0.583198 | 1.2:-86087933 | None:intergenic |
| GTCTGCTGGAGGAAAAGGGA+AGG | 0.583307 | 1.2:+86088665 | MS.gene74206:CDS |
| TATTGATTTAGAAGCAAACA+AGG | 0.584991 | 1.2:-86077753 | None:intergenic |
| AAAACAGTAGTTCTCCAGCC+AGG | 0.585984 | 1.2:-86089389 | None:intergenic |
| ACGGAGGCTGCACAAACTGA+TGG | 0.603336 | 1.2:+86088909 | MS.gene74206:CDS |
| AAGTGCAAACTTGATTGTGA+AGG | 0.605155 | 1.2:-86088033 | None:intergenic |
| TAGAGCATGGGGAAGCACAA+AGG | 0.605458 | 1.2:+86080907 | MS.gene74206:CDS |
| AGAGCTTAACCATTTGGTCA+CGG | 0.610389 | 1.2:+86088890 | MS.gene74206:intron |
| AGGCTGCACAAACTGATGGT+AGG | 0.613383 | 1.2:+86088913 | MS.gene74206:CDS |
| TTATGCATCTCGGCTTGCGG+GGG | 0.622308 | 1.2:-86078132 | None:intergenic |
| CCAGTTGGCTCTAGAGCATG+GGG | 0.627133 | 1.2:+86080896 | MS.gene74206:intron |
| TGTTTACACACCCTGAAGAG+TGG | 0.628835 | 1.2:-86088733 | None:intergenic |
| ACTTCCTGTTAGAAGACGAA+TGG | 0.634423 | 1.2:+86089318 | MS.gene74206:CDS |
| ATTTACTGACCATCAAGTCA+AGG | 0.654900 | 1.2:-86089413 | None:intergenic |
| TCTGCTGGAGGAAAAGGGAA+GGG | 0.677166 | 1.2:+86088666 | MS.gene74206:CDS |
| AGGGAGGTGAAGTCTGCTGG+AGG | 0.687293 | 1.2:+86088654 | MS.gene74206:intron |
| ATGGCTCGAGATCTCTTGCA+CGG | 0.704607 | 1.2:+86089337 | MS.gene74206:CDS |
| GCTTAACCATTTGGTCACGG+AGG | 0.739786 | 1.2:+86088893 | MS.gene74206:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 86077744 | 86089423 | 86077744 | ID=MS.gene74206 |
| chr1.2 | mRNA | 86077744 | 86089423 | 86077744 | ID=MS.gene74206.t1;Parent=MS.gene74206 |
| chr1.2 | exon | 86077744 | 86077814 | 86077744 | ID=MS.gene74206.t1.exon1;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86077744 | 86077814 | 86077744 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86078099 | 86078162 | 86078099 | ID=MS.gene74206.t1.exon2;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86078099 | 86078162 | 86078099 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86078300 | 86078407 | 86078300 | ID=MS.gene74206.t1.exon3;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86078300 | 86078407 | 86078300 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86079272 | 86079341 | 86079272 | ID=MS.gene74206.t1.exon4;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86079272 | 86079341 | 86079272 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86080900 | 86080940 | 86080900 | ID=MS.gene74206.t1.exon5;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86080900 | 86080940 | 86080900 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86081016 | 86081096 | 86081016 | ID=MS.gene74206.t1.exon6;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86081016 | 86081096 | 86081016 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86081618 | 86081653 | 86081618 | ID=MS.gene74206.t1.exon7;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86081618 | 86081653 | 86081618 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86087682 | 86087765 | 86087682 | ID=MS.gene74206.t1.exon8;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86087682 | 86087765 | 86087682 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86087912 | 86088045 | 86087912 | ID=MS.gene74206.t1.exon9;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86087912 | 86088045 | 86087912 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86088656 | 86088744 | 86088656 | ID=MS.gene74206.t1.exon10;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86088656 | 86088744 | 86088656 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86088892 | 86089040 | 86088892 | ID=MS.gene74206.t1.exon11;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86088892 | 86089040 | 86088892 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| chr1.2 | exon | 86089280 | 86089423 | 86089280 | ID=MS.gene74206.t1.exon12;Parent=MS.gene74206.t1 |
| chr1.2 | CDS | 86089280 | 86089423 | 86089280 | ID=cds.MS.gene74206.t1;Parent=MS.gene74206.t1 |
| Gene Sequence |
| Protein sequence |
