Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74314.t1 | PNY07851.1 | 34.3 | 216 | 140 | 2 | 11 | 224 | 9 | 224 | 9.00E-30 | 140.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74314.t1 | A0A2K3NXT8 | 34.3 | 216 | 140 | 2 | 11 | 224 | 9 | 224 | 6.5e-30 | 140.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene74314.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74314.t1 | MTR_4g123470 | 27.749 | 191 | 138 | 0 | 19 | 209 | 20 | 210 | 7.82e-26 | 100 |
| MS.gene74314.t1 | MTR_4g085790 | 24.623 | 199 | 149 | 1 | 26 | 224 | 24 | 221 | 3.55e-15 | 72.0 |
| MS.gene74314.t1 | MTR_7g025150 | 24.615 | 195 | 146 | 1 | 30 | 224 | 28 | 221 | 4.96e-15 | 71.6 |
| MS.gene74314.t1 | MTR_1g054495 | 23.316 | 193 | 147 | 1 | 18 | 210 | 16 | 207 | 1.27e-14 | 70.9 |
| MS.gene74314.t1 | MTR_0014s0220 | 25.616 | 203 | 133 | 5 | 13 | 214 | 628 | 813 | 2.07e-14 | 72.0 |
| MS.gene74314.t1 | MTR_1g090753 | 32.632 | 95 | 64 | 0 | 23 | 117 | 38 | 132 | 7.99e-14 | 67.4 |
| MS.gene74314.t1 | MTR_4g053550 | 44.444 | 72 | 40 | 0 | 26 | 97 | 47 | 118 | 1.73e-13 | 65.5 |
| MS.gene74314.t1 | MTR_5g074790 | 31.731 | 104 | 71 | 0 | 15 | 118 | 160 | 263 | 7.81e-13 | 66.2 |
| MS.gene74314.t1 | MTR_1g054485 | 23.353 | 167 | 128 | 0 | 23 | 189 | 21 | 187 | 1.57e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATGTAATGAAGGTTTCT+AGG | 0.257949 | 3.1:+19868715 | MS.gene74314:CDS |
| AATGATCTTTATGAAGTTCT+TGG | 0.265359 | 3.1:+19868246 | MS.gene74314:CDS |
| GAACTTAACTTTGTGTCTAT+TGG | 0.280740 | 3.1:-19868146 | None:intergenic |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 3.1:+19868247 | MS.gene74314:CDS |
| TGATAACCTGCAGCCATATA+AGG | 0.324757 | 3.1:-19868388 | None:intergenic |
| AATGACCTTAACTCCTTATA+TGG | 0.332349 | 3.1:+19868375 | MS.gene74314:CDS |
| CTGAGGGATGGATCAACTTA+AGG | 0.380199 | 3.1:+19868197 | MS.gene74314:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 3.1:-19868282 | None:intergenic |
| AGGACTAAACATAGGGATAA+TGG | 0.397283 | 3.1:+19867931 | MS.gene74314:CDS |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 3.1:+19868680 | MS.gene74314:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 3.1:+19868495 | MS.gene74314:intron |
| TTCCAATTCACTGCATTTCG+TGG | 0.438395 | 3.1:+19868280 | MS.gene74314:CDS |
| TGCAGCCATATAAGGAGTTA+AGG | 0.444382 | 3.1:-19868380 | None:intergenic |
| TATGGCTGCAGGTTATCAAT+TGG | 0.445570 | 3.1:+19868393 | MS.gene74314:CDS |
| GAGTTAAGGTCATTTCATAA+TGG | 0.446526 | 3.1:-19868366 | None:intergenic |
| GGTGATTACATTACAAGCTC+AGG | 0.451144 | 3.1:+19868516 | MS.gene74314:CDS |
| GTTGTTATCAATACAATGTC+TGG | 0.455307 | 3.1:+19867892 | None:intergenic |
| ATTAATATGTATCGACCTAC+AGG | 0.482580 | 3.1:+19868549 | MS.gene74314:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.485967 | 3.1:+19868198 | MS.gene74314:CDS |
| CTGGTGGTGGATCAACTTCA+AGG | 0.486585 | 3.1:+19867911 | MS.gene74314:CDS |
| AAGTACATTATATGTAATGA+AGG | 0.486831 | 3.1:+19868707 | MS.gene74314:CDS |
| TGCAAAGACAATGGATTCCA+TGG | 0.493056 | 3.1:+19868651 | MS.gene74314:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.501456 | 3.1:+19868382 | MS.gene74314:CDS |
| ATGTTAACTCATTGCCATCA+TGG | 0.509173 | 3.1:+19868311 | MS.gene74314:CDS |
| ATAAAATGCTCAACAAATGA+AGG | 0.510176 | 3.1:-19868082 | None:intergenic |
| CAACTTCAAGGACTAAACAT+AGG | 0.510435 | 3.1:+19867923 | MS.gene74314:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 3.1:+19868336 | MS.gene74314:CDS |
| ATATCTCATTTACAGTCGTA+TGG | 0.533053 | 3.1:+19868049 | MS.gene74314:intron |
| AAGACAATGGATTCCATGGT+GGG | 0.550114 | 3.1:+19868655 | MS.gene74314:CDS |
| ATCCATCCCTCAGTCAGAAG+TGG | 0.551673 | 3.1:-19868187 | None:intergenic |
| ATCAATACAATGTCTGGTGG+TGG | 0.560856 | 3.1:+19867898 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 3.1:+19868248 | MS.gene74314:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 3.1:-19868325 | None:intergenic |
| ATCCACTTCTGACTGAGGGA+TGG | 0.571896 | 3.1:+19868185 | MS.gene74314:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.574230 | 3.1:+19868615 | MS.gene74314:CDS |
| AACTTCAAGGACTAAACATA+GGG | 0.584439 | 3.1:+19867924 | MS.gene74314:CDS |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 3.1:-19868564 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 3.1:-19868574 | None:intergenic |
| TGAACATCCACTTCTGACTG+AGG | 0.599795 | 3.1:+19868180 | MS.gene74314:CDS |
| CAACAAAGATTGGCAGTGGG+TGG | 0.601376 | 3.1:+19868619 | MS.gene74314:CDS |
| TACTGCTTATATCATCAATG+AGG | 0.608119 | 3.1:+19867963 | MS.gene74314:CDS |
| TGTGATGTACTAATAAGCGA+TGG | 0.609250 | 3.1:+19868585 | MS.gene74314:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 3.1:-19868668 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.634933 | 3.1:+19868654 | MS.gene74314:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 3.1:+19868328 | MS.gene74314:CDS |
| GAACATCCACTTCTGACTGA+GGG | 0.646274 | 3.1:+19868181 | MS.gene74314:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.648905 | 3.1:+19868656 | MS.gene74314:CDS |
| AGGCAACAAAGATTGGCAGT+GGG | 0.670518 | 3.1:+19868616 | MS.gene74314:CDS |
| GTTATCAATACAATGTCTGG+TGG | 0.684260 | 3.1:+19867895 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTACATTATATGTAATGA+AGG | + | chr3.1:19868707-19868726 | MS.gene74314:CDS | 20.0% |
| !! | ATTGCTCAACATAAAATAAT+AGG | - | chr3.1:19867998-19868017 | None:intergenic | 20.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | + | chr3.1:19868246-19868265 | MS.gene74314:CDS | 25.0% |
| ! | ATAAAATGCTCAACAAATGA+AGG | - | chr3.1:19868085-19868104 | None:intergenic | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | + | chr3.1:19868247-19868266 | MS.gene74314:CDS | 25.0% |
| ! | TAGAGATGTATCATTTGATA+AGG | - | chr3.1:19868434-19868453 | None:intergenic | 25.0% |
| ! | TATATGTAATGAAGGTTTCT+AGG | + | chr3.1:19868715-19868734 | MS.gene74314:CDS | 25.0% |
| !! | AACAAATTTTGCAAAGACAA+TGG | + | chr3.1:19868642-19868661 | MS.gene74314:CDS | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | chr3.1:19868680-19868699 | MS.gene74314:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | chr3.1:19868609-19868628 | MS.gene74314:CDS | 30.0% | |
| AACTTCAAGGACTAAACATA+GGG | + | chr3.1:19867924-19867943 | MS.gene74314:CDS | 30.0% | |
| AATAAGCGATGGAAAAAAGA+AGG | + | chr3.1:19868596-19868615 | MS.gene74314:CDS | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | + | chr3.1:19868375-19868394 | MS.gene74314:CDS | 30.0% | |
| ATATCTCATTTACAGTCGTA+TGG | + | chr3.1:19868049-19868068 | MS.gene74314:intron | 30.0% | |
| ATCATTTGATAAGGAGTTGT+TGG | - | chr3.1:19868425-19868444 | None:intergenic | 30.0% | |
| GAACTTAACTTTGTGTCTAT+TGG | - | chr3.1:19868149-19868168 | None:intergenic | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | - | chr3.1:19868369-19868388 | None:intergenic | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | + | chr3.1:19867963-19867982 | MS.gene74314:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | - | chr3.1:19868577-19868596 | None:intergenic | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | + | chr3.1:19868248-19868267 | MS.gene74314:CDS | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | + | chr3.1:19868208-19868227 | MS.gene74314:CDS | 30.0% |
| ! | ATTAATATGTATCGACCTAC+AGG | + | chr3.1:19868549-19868568 | MS.gene74314:CDS | 30.0% |
| ! | GAGCATTTTATTCACAAACT+TGG | + | chr3.1:19868094-19868113 | MS.gene74314:CDS | 30.0% |
| AGGACTAAACATAGGGATAA+TGG | + | chr3.1:19867931-19867950 | MS.gene74314:CDS | 35.0% | |
| ATGTTAACTCATTGCCATCA+TGG | + | chr3.1:19868311-19868330 | MS.gene74314:CDS | 35.0% | |
| CAACTTCAAGGACTAAACAT+AGG | + | chr3.1:19867923-19867942 | MS.gene74314:CDS | 35.0% | |
| TGTGATGTACTAATAAGCGA+TGG | + | chr3.1:19868585-19868604 | MS.gene74314:CDS | 35.0% | |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | + | chr3.1:19868212-19868231 | MS.gene74314:CDS | 35.0% |
| CACTTACAAGCAAGGAATTG+AGG | + | chr3.1:19868336-19868355 | MS.gene74314:CDS | 40.0% | |
| GGTGATTACATTACAAGCTC+AGG | + | chr3.1:19868516-19868535 | MS.gene74314:CDS | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | + | chr3.1:19868393-19868412 | MS.gene74314:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | + | chr3.1:19868198-19868217 | MS.gene74314:CDS | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | - | chr3.1:19868391-19868410 | None:intergenic | 40.0% | |
| TGCAGCCATATAAGGAGTTA+AGG | - | chr3.1:19868383-19868402 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | + | chr3.1:19868382-19868401 | MS.gene74314:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | + | chr3.1:19868280-19868299 | MS.gene74314:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | + | chr3.1:19868654-19868673 | MS.gene74314:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | + | chr3.1:19868655-19868674 | MS.gene74314:CDS | 40.0% |
| ! | TGCAAAGACAATGGATTCCA+TGG | + | chr3.1:19868651-19868670 | MS.gene74314:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | + | chr3.1:19868495-19868514 | MS.gene74314:intron | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | chr3.1:19868615-19868634 | MS.gene74314:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | - | chr3.1:19868671-19868690 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | + | chr3.1:19868616-19868635 | MS.gene74314:CDS | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | + | chr3.1:19868197-19868216 | MS.gene74314:CDS | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | + | chr3.1:19868181-19868200 | MS.gene74314:CDS | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | - | chr3.1:19868285-19868304 | None:intergenic | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | chr3.1:19868328-19868347 | MS.gene74314:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | + | chr3.1:19868180-19868199 | MS.gene74314:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | chr3.1:19868328-19868347 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | + | chr3.1:19868656-19868675 | MS.gene74314:CDS | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | + | chr3.1:19868185-19868204 | MS.gene74314:CDS | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | - | chr3.1:19868190-19868209 | None:intergenic | 50.0% | |
| CAACAAAGATTGGCAGTGGG+TGG | + | chr3.1:19868619-19868638 | MS.gene74314:CDS | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | chr3.1:19868567-19868586 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCAACTTCA+AGG | + | chr3.1:19867911-19867930 | MS.gene74314:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 19867907 | 19868743 | 19867907 | ID=MS.gene74314 |
| chr3.1 | mRNA | 19867907 | 19868743 | 19867907 | ID=MS.gene74314.t1;Parent=MS.gene74314 |
| chr3.1 | exon | 19867907 | 19867984 | 19867907 | ID=MS.gene74314.t1.exon1;Parent=MS.gene74314.t1 |
| chr3.1 | CDS | 19867907 | 19867984 | 19867907 | ID=cds.MS.gene74314.t1;Parent=MS.gene74314.t1 |
| chr3.1 | exon | 19868064 | 19868414 | 19868064 | ID=MS.gene74314.t1.exon2;Parent=MS.gene74314.t1 |
| chr3.1 | CDS | 19868064 | 19868414 | 19868064 | ID=cds.MS.gene74314.t1;Parent=MS.gene74314.t1 |
| chr3.1 | exon | 19868498 | 19868743 | 19868498 | ID=MS.gene74314.t1.exon3;Parent=MS.gene74314.t1 |
| chr3.1 | CDS | 19868498 | 19868743 | 19868498 | ID=cds.MS.gene74314.t1;Parent=MS.gene74314.t1 |
| Gene Sequence |
| Protein sequence |
