AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74585.t1 MTR_1g031930 50.418 359 136 6 1 341 1 335 2.28e-105 313
MS.gene74585.t1 MTR_1g031930 49.446 271 119 6 85 341 8 274 7.84e-70 220
MS.gene74585.t1 MTR_2g029260 83.621 116 19 0 180 295 3 118 2.52e-51 166
MS.gene74585.t1 MTR_6g069870 31.818 374 176 12 37 344 2 362 1.25e-39 144
MS.gene74585.t1 MTR_6g069870 31.461 356 165 12 37 326 2 344 4.74e-34 128
MS.gene74585.t1 MTR_6g069870 32.990 291 166 10 37 315 2 275 1.35e-28 113
MS.gene74585.t1 MTR_2g042900 35.465 172 78 5 174 340 97 240 2.52e-16 78.2
MS.gene74585.t1 MTR_2g042900 34.524 168 87 6 174 326 97 256 2.83e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74585.t1 AT3G17860 33.858 381 174 16 1 345 1 339 1.80e-35 132
MS.gene74585.t1 AT3G17860 34.104 346 154 14 35 345 20 326 1.21e-31 122
MS.gene74585.t1 AT3G17860 34.111 343 152 14 38 345 3 306 3.56e-31 120
MS.gene74585.t1 AT3G17860 34.111 343 152 14 38 345 3 306 3.56e-31 120
MS.gene74585.t1 AT1G48500 31.395 344 183 11 1 342 1 293 1.58e-26 107
MS.gene74585.t1 AT1G70700 37.004 227 97 6 113 333 58 244 1.30e-22 95.9
MS.gene74585.t1 AT1G70700 34.389 221 87 7 113 333 58 220 1.66e-21 92.4
MS.gene74585.t1 AT1G48500 30.581 327 175 10 1 326 1 276 4.10e-21 92.0
MS.gene74585.t1 AT1G70700 36.364 220 94 6 113 326 58 237 4.68e-19 86.3
MS.gene74585.t1 AT1G48500 30.345 290 154 9 38 326 4 246 1.40e-16 78.6

Find 94 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCTTGTTAATGTTATA+TGG 0.220633 2.3:-60363433 None:intergenic
CGAAACTGAATTCGTTTGAT+TGG 0.273105 2.3:-60362011 None:intergenic
ATGATCCATCATCACCATTA+TGG 0.297946 2.3:-60360538 None:intergenic
GAGGAGGCTGGTGCATTTGC+AGG 0.320510 2.3:-60362817 None:intergenic
CCCACCATTGGTGCCGTTGC+TGG 0.348704 2.3:+60362151 MS.gene74585:CDS
AGCAGATACTTTGTTGGAAA+AGG 0.357605 2.3:-60361292 None:intergenic
CATAAAACCAGCAGATGTTA+TGG 0.358564 2.3:-60361376 None:intergenic
AGTATGCGATGAAATAGATA+TGG 0.369654 2.3:-60362854 None:intergenic
TTACTAGAATCAAATGCATT+TGG 0.371663 2.3:-60361402 None:intergenic
ATATGAAGCTGTCACTGCTT+GGG 0.380060 2.3:+60362095 MS.gene74585:CDS
GGAGGCTCGGCATTGCTAAC+AGG 0.384286 2.3:-60362952 None:intergenic
TTTAACTGCGAGAAATTCAT+TGG 0.399647 2.3:-60362908 None:intergenic
GGTGATTGAGGTGCTGTAAC+AGG 0.402330 2.3:-60362124 None:intergenic
TGTAGGCTTCACAAAGGGTT+CGG 0.418966 2.3:+60361257 MS.gene74585:intron
TTTCTCGCAGTTAAAACAAC+TGG 0.425409 2.3:+60362916 MS.gene74585:CDS
CATAGCACAGACAGAACTTC+AGG 0.434059 2.3:+60362765 MS.gene74585:CDS
CGCATAGAAGATGGTAAGTT+GGG 0.437429 2.3:-60362316 None:intergenic
GGCACAAGCTATCCTGTTGT+TGG 0.441454 2.3:+60362714 MS.gene74585:intron
GCCATTACCATGCTGGCGTC+AGG 0.443156 2.3:+60362988 MS.gene74585:CDS
GTAGGCTTCACAAAGGGTTC+GGG 0.445204 2.3:+60361258 MS.gene74585:intron
ACGGCACCAATGGTGGGAAC+AGG 0.446208 2.3:-60362145 None:intergenic
ACTCTCTGCTGCTGAACCTC+AGG 0.447860 2.3:+60361428 MS.gene74585:CDS
CACCATTCCTAGCCAACAAC+AGG 0.452637 2.3:-60362726 None:intergenic
ATATCTATTTCATCGCATAC+TGG 0.462263 2.3:+60362856 MS.gene74585:CDS
TAGAAATCTTTCAATCATGA+TGG 0.465598 2.3:+60361920 MS.gene74585:intron
GCAAATGCACCAGCCTCCTC+TGG 0.467713 2.3:+60362820 MS.gene74585:CDS
AATATGAAGCTGTCACTGCT+TGG 0.470682 2.3:+60362094 MS.gene74585:CDS
GAGGAGGAAATCAACCATAA+TGG 0.472291 2.3:+60360524 MS.gene74585:CDS
GCAGATACTTTGTTGGAAAA+GGG 0.473482 2.3:-60361291 None:intergenic
TCGCATACTGGTGCACAGTC+AGG 0.473936 2.3:+60362868 MS.gene74585:CDS
ATAAAACCAGCAGATGTTAT+GGG 0.478111 2.3:-60361375 None:intergenic
TCTGATCCCATAACATCTGC+TGG 0.481214 2.3:+60361369 MS.gene74585:CDS
TGCATTTGCAGGTTGACTCA+CGG 0.482777 2.3:-60362806 None:intergenic
ACCTGACGCCAGCATGGTAA+TGG 0.485087 2.3:-60362989 None:intergenic
GTAATGGCATCGATTACTCT+TGG 0.489598 2.3:-60362973 None:intergenic
TCAGGCTCGGAAAGCATCCT+TGG 0.496291 2.3:+60363167 MS.gene74585:CDS
CAATCACCTGTTCCCACCAT+TGG 0.496425 2.3:+60362139 MS.gene74585:CDS
AAGCTATCCTGTTGTTGGCT+AGG 0.504599 2.3:+60362719 MS.gene74585:CDS
ATCGATTACTCTTGGAGGCT+CGG 0.505842 2.3:-60362965 None:intergenic
CAACTTACCATCTTCTATGC+GGG 0.506683 2.3:+60362318 MS.gene74585:CDS
ATCTTCACATCATGTTGTAC+AGG 0.507813 2.3:-60361977 None:intergenic
TATGAAGCTGTCACTGCTTG+GGG 0.509930 2.3:+60362096 MS.gene74585:CDS
AAGAGAGATACCTTGTCGGC+AGG 0.512991 2.3:-60362366 None:intergenic
TCCAGCAACGGCACCAATGG+TGG 0.513212 2.3:-60362152 None:intergenic
GGCATTGCTAACAGGAGTGA+TGG 0.514325 2.3:-60362944 None:intergenic
CCGCATAGAAGATGGTAAGT+TGG 0.523235 2.3:-60362317 None:intergenic
GCATTTGCAGGTTGACTCAC+GGG 0.524091 2.3:-60362805 None:intergenic
GCAGTTACAAAATGATTCCT+CGG 0.526505 2.3:-60362049 None:intergenic
AGTTGGGAAGAAAGCAGAGA+TGG 0.527888 2.3:-60362300 None:intergenic
ATCCTGTTGTTGGCTAGGAA+TGG 0.530502 2.3:+60362724 MS.gene74585:CDS
AACAAAGAGAGATACCTTGT+CGG 0.533016 2.3:-60362370 None:intergenic
CTCACGGGCACACCGTCACC+AGG 0.540218 2.3:-60362790 None:intergenic
CAGTTACAAAATGATTCCTC+GGG 0.542977 2.3:-60362048 None:intergenic
TGGTTGAGCAGATACTTTGT+TGG 0.544131 2.3:-60361298 None:intergenic
GACGGTACCCGCATAGAAGA+TGG 0.552065 2.3:-60362325 None:intergenic
CTTGGATGACCAGAGGAGGC+TGG 0.553563 2.3:-60362829 None:intergenic
CAATGACGTCCCTGCCGACA+AGG 0.559227 2.3:+60362356 MS.gene74585:CDS
ATGCAGCGATACCTCAGGCT+CGG 0.560553 2.3:+60363154 MS.gene74585:intron
GACGTCATTGAAAACATTGA+CGG 0.563003 2.3:-60362343 None:intergenic
AGAAATCTTTCAATCATGAT+GGG 0.564188 2.3:+60361921 MS.gene74585:intron
AGAGAGATACCTTGTCGGCA+GGG 0.565241 2.3:-60362365 None:intergenic
ATGGCATCGATTACTCTTGG+AGG 0.566283 2.3:-60362970 None:intergenic
AATCGATGCCATTACCATGC+TGG 0.572186 2.3:+60362981 MS.gene74585:CDS
CAGACAGAACTTCAGGCACC+TGG 0.577240 2.3:+60362772 MS.gene74585:CDS
ACTGGTGCACAGTCAGGCAG+TGG 0.578273 2.3:+60362874 MS.gene74585:CDS
CATTCCAGCAACGGCACCAA+TGG 0.580478 2.3:-60362155 None:intergenic
GTATGCGATGAAATAGATAT+GGG 0.584760 2.3:-60362853 None:intergenic
CCATGGTACTGCTAAGGAGG+AGG 0.587651 2.3:+60360508 MS.gene74585:CDS
GAACTTCAGGCACCTGGTGA+CGG 0.589320 2.3:+60362778 MS.gene74585:CDS
ACTGCTGGTCAGAATATGAA+TGG 0.602374 2.3:+60362067 MS.gene74585:CDS
GCATTGCTAACAGGAGTGAT+GGG 0.603727 2.3:-60362943 None:intergenic
ACTCCATGGTACTGCTAAGG+AGG 0.604086 2.3:+60360505 MS.gene74585:CDS
CGTTGATGAACTCATTCCCG+AGG 0.604339 2.3:+60362032 MS.gene74585:CDS
TTCTAGAAAAGCGCAAGGAG+AGG 0.607349 2.3:+60363196 MS.gene74585:CDS
TCTTTCAATCATGATGGGCA+AGG 0.608612 2.3:+60361926 MS.gene74585:CDS
GGCTAGGAATGGTGTATCAG+TGG 0.612360 2.3:+60362735 MS.gene74585:CDS
ACTACTCCATGGTACTGCTA+AGG 0.614665 2.3:+60360502 MS.gene74585:CDS
GATGAAATAGATATGGGACT+TGG 0.616222 2.3:-60362847 None:intergenic
CCTCCTCCTTAGCAGTACCA+TGG 0.619904 2.3:-60360508 None:intergenic
CGATTCAGTCATTCCAGCAA+CGG 0.622512 2.3:-60362164 None:intergenic
CAGCATACCTGACGCCAGCA+TGG 0.631084 2.3:-60362995 None:intergenic
ACCTCAAAAGACATCAAATG+TGG 0.632234 2.3:-60361318 None:intergenic
CCAGCAACGGCACCAATGGT+GGG 0.634359 2.3:-60362151 None:intergenic
AAGGATGCTTTCCGAGCCTG+AGG 0.641286 2.3:-60363165 None:intergenic
GTATGTGTAGGCTTCACAAA+GGG 0.645767 2.3:+60361252 MS.gene74585:intron
CAAAGGGTTCGGGTGCACAA+TGG 0.652681 2.3:+60361268 MS.gene74585:CDS
TTCAATCATGATGGGCAAGG+CGG 0.653055 2.3:+60361929 MS.gene74585:CDS
GGTATGTGTAGGCTTCACAA+AGG 0.665915 2.3:+60361251 MS.gene74585:intron
ATGAAGCTGTCACTGCTTGG+GGG 0.670593 2.3:+60362097 MS.gene74585:CDS
CCAACTTACCATCTTCTATG+CGG 0.674088 2.3:+60362317 MS.gene74585:CDS
ATGGTGGGAACAGGTGATTG+AGG 0.679435 2.3:-60362136 None:intergenic
GGGACTTGGATGACCAGAGG+AGG 0.679930 2.3:-60362833 None:intergenic
AAAATGGTGAGAGATTTCAT+GGG 0.689320 2.3:+60360458 None:intergenic
TATGGGACTTGGATGACCAG+AGG 0.694039 2.3:-60362836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTATTTTATTTTAATTTG+AGG - chr2.3:60361787-60361806 None:intergenic 10.0%
!!! ATTTTACTTTTATAAAGAAA+AGG - chr2.3:60363234-60363253 None:intergenic 10.0%
!! AAATTATTAAATGTGTAGAA+CGG - chr2.3:60363119-60363138 None:intergenic 15.0%
!! TAAATAAATACAAGGTAAAA+AGG + chr2.3:60362410-60362429 MS.gene74585:intron 15.0%
!! TTTACCTTGTATTTATTTAT+AGG - chr2.3:60362409-60362428 None:intergenic 15.0%
!!! AGAATTAATTTTGAAATGTT+TGG - chr2.3:60361120-60361139 None:intergenic 15.0%
!!! TAATTTTATGATTGACTTTT+AGG + chr2.3:60362205-60362224 MS.gene74585:intron 15.0%
!! AAGGAAAAAAAAATGAATTG+TGG - chr2.3:60362436-60362455 None:intergenic 20.0%
!! AATAACAAACAATCAATAGA+GGG - chr2.3:60361896-60361915 None:intergenic 20.0%
!! ACATGTTTCTCATATAAAAA+TGG + chr2.3:60360985-60361004 MS.gene74585:intron 20.0%
!! AGATTGAAAAATTCACAATA+TGG - chr2.3:60361206-60361225 None:intergenic 20.0%
!! GATTTCTTGTTAATGTTATA+TGG - chr2.3:60363436-60363455 None:intergenic 20.0%
!! TAATAACAAACAATCAATAG+AGG - chr2.3:60361897-60361916 None:intergenic 20.0%
!! TCGTTAACTTAAGTAAAATA+AGG + chr2.3:60361662-60361681 MS.gene74585:intron 20.0%
!! TTGTTTATTGAAATCTTGTT+TGG + chr2.3:60363053-60363072 MS.gene74585:intron 20.0%
!!! AAGTACTTTTCAATTTTCTT+TGG + chr2.3:60360575-60360594 MS.gene74585:intron 20.0%
!!! AATCTATTTTTTATCCCTTA+AGG + chr2.3:60361221-60361240 MS.gene74585:intron 20.0%
!!! TGTTTATTGAAATCTTGTTT+GGG + chr2.3:60363054-60363073 MS.gene74585:intron 20.0%
!!! TTCAAATTCAACTTTTGTAA+CGG - chr2.3:60360918-60360937 None:intergenic 20.0%
! AAAAGTAATGTGTGTAGAAA+TGG - chr2.3:60363284-60363303 None:intergenic 25.0%
! AGAAATCTTTCAATCATGAT+GGG + chr2.3:60361921-60361940 MS.gene74585:intron 25.0%
! ATGGTACTCTAAACAAAAAA+CGG - chr2.3:60360605-60360624 None:intergenic 25.0%
! CTTGTATAAGATATTCCTTA+AGG + chr2.3:60360772-60360791 MS.gene74585:intron 25.0%
! TAGAAATCTTTCAATCATGA+TGG + chr2.3:60361920-60361939 MS.gene74585:intron 25.0%
! TCTGCCTATAAATAAATACA+AGG + chr2.3:60362402-60362421 MS.gene74585:intron 25.0%
! TGTATTTATTTATAGGCAGA+AGG - chr2.3:60362402-60362421 None:intergenic 25.0%
! TTAATGCTAAGATCAATTGT+AGG + chr2.3:60361022-60361041 MS.gene74585:intron 25.0%
! TTACTAGAATCAAATGCATT+TGG - chr2.3:60361405-60361424 None:intergenic 25.0%
!! GAATTGATTCAACTTCAAAT+TGG - chr2.3:60361066-60361085 None:intergenic 25.0%
!! GTTTATTGAAATCTTGTTTG+GGG + chr2.3:60363055-60363074 MS.gene74585:intron 25.0%
!! TTGAAATGTTTGGATCTTTT+CGG - chr2.3:60361110-60361129 None:intergenic 25.0%
!! TTTTTCGTTAACCAAGTTAT+TGG - chr2.3:60362647-60362666 None:intergenic 25.0%
!!! TATATGTTTGGTTTCACTTT+TGG - chr2.3:60361170-60361189 None:intergenic 25.0%
!!! TCATGAGTGTTTAATTTGAT+TGG + chr2.3:60360637-60360656 MS.gene74585:intron 25.0%
!!! TGTGACTTTTTTTACATTGA+CGG + chr2.3:60361624-60361643 MS.gene74585:intron 25.0%
AAAGAAAAAGAACTACTCCA+TGG + chr2.3:60360491-60360510 MS.gene74585:CDS 30.0%
AACCAAACATATACACTTAG+TGG + chr2.3:60361177-60361196 MS.gene74585:intron 30.0%
ACCATCCTAGATTAGATTTA+AGG + chr2.3:60363254-60363273 MS.gene74585:intron 30.0%
ACTTGGTTAACGAAAAAACT+TGG + chr2.3:60362650-60362669 MS.gene74585:intron 30.0%
AGTATGCGATGAAATAGATA+TGG - chr2.3:60362857-60362876 None:intergenic 30.0%
ATAAAACCAGCAGATGTTAT+GGG - chr2.3:60361378-60361397 None:intergenic 30.0%
ATATCTATTTCATCGCATAC+TGG + chr2.3:60362856-60362875 MS.gene74585:CDS 30.0%
ATCAGTTTGACAAAATTTGG+TGG - chr2.3:60360855-60360874 None:intergenic 30.0%
ATCCACTAAGTGTATATGTT+TGG - chr2.3:60361182-60361201 None:intergenic 30.0%
CATTTAGTATTGTGTTTGCA+GGG + chr2.3:60363396-60363415 MS.gene74585:intron 30.0%
CCATCCTAGATTAGATTTAA+GGG + chr2.3:60363255-60363274 MS.gene74585:intron 30.0%
CTAACTGCAATAATGAGAAA+TGG - chr2.3:60363027-60363046 None:intergenic 30.0%
GCTTATGAACAAGTAACATT+TGG + chr2.3:60361692-60361711 MS.gene74585:intron 30.0%
GTATGCGATGAAATAGATAT+GGG - chr2.3:60362856-60362875 None:intergenic 30.0%
TTAATGATCGACGATTATGA+CGG + chr2.3:60361593-60361612 MS.gene74585:intron 30.0%
TTTAACTGCGAGAAATTCAT+TGG - chr2.3:60362911-60362930 None:intergenic 30.0%
TTTATCCCTTAAGGTTTAGT+GGG + chr2.3:60361230-60361249 MS.gene74585:intron 30.0%
! CCAAATTTTGTCAAACTGAT+CGG + chr2.3:60360855-60360874 MS.gene74585:intron 30.0%
! TTTTATCCCTTAAGGTTTAG+TGG + chr2.3:60361229-60361248 MS.gene74585:intron 30.0%
!! TAGATTGATGGTGAATTTGA+TGG + chr2.3:60360807-60360826 MS.gene74585:intron 30.0%
!! TGAGTGTTTAATTTGATTGG+TGG + chr2.3:60360640-60360659 MS.gene74585:intron 30.0%
AACAAAGAGAGATACCTTGT+CGG - chr2.3:60362373-60362392 None:intergenic 35.0%
ACAAACAATCAATAGAGGGA+AGG - chr2.3:60361892-60361911 None:intergenic 35.0%
ACATACCCACTAAACCTTAA+GGG - chr2.3:60361238-60361257 None:intergenic 35.0%
ACCATAGTCAAGCTTAATTG+TGG + chr2.3:60362678-60362697 MS.gene74585:intron 35.0%
ACCTCAAAAGACATCAAATG+TGG - chr2.3:60361321-60361340 None:intergenic 35.0%
AGATCAATTGTAGGAGTCAA+AGG + chr2.3:60361031-60361050 MS.gene74585:intron 35.0%
AGCAGATACTTTGTTGGAAA+AGG - chr2.3:60361295-60361314 None:intergenic 35.0%
ATCTTCACATCATGTTGTAC+AGG - chr2.3:60361980-60361999 None:intergenic 35.0%
ATGATCCATCATCACCATTA+TGG - chr2.3:60360541-60360560 None:intergenic 35.0%
ATTAAGCTTGACTATGGTCA+AGG - chr2.3:60362676-60362695 None:intergenic 35.0%
CAAACAATCAATAGAGGGAA+GGG - chr2.3:60361891-60361910 None:intergenic 35.0%
CAGTTACAAAATGATTCCTC+GGG - chr2.3:60362051-60362070 None:intergenic 35.0%
CATAAAACCAGCAGATGTTA+TGG - chr2.3:60361379-60361398 None:intergenic 35.0%
CCCTTAAATCTAATCTAGGA+TGG - chr2.3:60363258-60363277 None:intergenic 35.0%
CCGATCAGTTTGACAAAATT+TGG - chr2.3:60360858-60360877 None:intergenic 35.0%
CGAAACTGAATTCGTTTGAT+TGG - chr2.3:60362014-60362033 None:intergenic 35.0%
CTTGATCACCAACATTACAT+TGG + chr2.3:60362467-60362486 MS.gene74585:intron 35.0%
GACGTCATTGAAAACATTGA+CGG - chr2.3:60362346-60362365 None:intergenic 35.0%
GATGAAATAGATATGGGACT+TGG - chr2.3:60362850-60362869 None:intergenic 35.0%
GCAGATACTTTGTTGGAAAA+GGG - chr2.3:60361294-60361313 None:intergenic 35.0%
GCAGTTACAAAATGATTCCT+CGG - chr2.3:60362052-60362071 None:intergenic 35.0%
GCATTTAGTATTGTGTTTGC+AGG + chr2.3:60363395-60363414 MS.gene74585:intron 35.0%
GTTGCCCTTAAATCTAATCT+AGG - chr2.3:60363262-60363281 None:intergenic 35.0%
TCAGTGCATGTTTAGATTGA+TGG + chr2.3:60360795-60360814 MS.gene74585:intron 35.0%
TTTCTCGCAGTTAAAACAAC+TGG + chr2.3:60362916-60362935 MS.gene74585:CDS 35.0%
! AATTGTGGCACTATTTCTTC+AGG + chr2.3:60362693-60362712 MS.gene74585:intron 35.0%
! ACCACATTTGATGTCTTTTG+AGG + chr2.3:60361317-60361336 MS.gene74585:CDS 35.0%
! AGGAATCATTTTGTAACTGC+TGG + chr2.3:60362052-60362071 MS.gene74585:CDS 35.0%
! AGTTTAAAGCCTAAACAGCA+CGG - chr2.3:60361470-60361489 None:intergenic 35.0%
! ATCAACCATAATGGTGATGA+TGG + chr2.3:60360533-60360552 MS.gene74585:intron 35.0%
! TTTTACATTGACGGTGTCTA+TGG + chr2.3:60361633-60361652 MS.gene74585:intron 35.0%
!! CAATGTAATGTTGGTGATCA+AGG - chr2.3:60362469-60362488 None:intergenic 35.0%
AATATGAAGCTGTCACTGCT+TGG + chr2.3:60362094-60362113 MS.gene74585:CDS 40.0%
AATCAATAGAGGGAAGGGAA+GGG - chr2.3:60361886-60361905 None:intergenic 40.0%
ACTGCTGGTCAGAATATGAA+TGG + chr2.3:60362067-60362086 MS.gene74585:CDS 40.0%
ATATGAAGCTGTCACTGCTT+GGG + chr2.3:60362095-60362114 MS.gene74585:CDS 40.0%
ATGGGGAAAAAATGGTTGAG+AGG + chr2.3:60362549-60362568 MS.gene74585:intron 40.0%
CAACTTACCATCTTCTATGC+GGG + chr2.3:60362318-60362337 MS.gene74585:CDS 40.0%
CACATACCCACTAAACCTTA+AGG - chr2.3:60361239-60361258 None:intergenic 40.0%
CCAACTTACCATCTTCTATG+CGG + chr2.3:60362317-60362336 MS.gene74585:CDS 40.0%
CCAATCAGATTAGAACACAC+TGG - chr2.3:60361723-60361742 None:intergenic 40.0%
CCAGTGTGTTCTAATCTGAT+TGG + chr2.3:60361720-60361739 MS.gene74585:intron 40.0%
CGCATAGAAGATGGTAAGTT+GGG - chr2.3:60362319-60362338 None:intergenic 40.0%
CTCATGAATGTTTGCATGCA+TGG - chr2.3:60360624-60360643 None:intergenic 40.0%
GAATCAGAGACCTAGCTTAA+GGG - chr2.3:60362518-60362537 None:intergenic 40.0%
GAGGAGGAAATCAACCATAA+TGG + chr2.3:60360524-60360543 MS.gene74585:CDS 40.0%
GCCACAATTAAGCTTGACTA+TGG - chr2.3:60362682-60362701 None:intergenic 40.0%
GGTATCGCTGCATAAAAGAT+TGG - chr2.3:60363147-60363166 None:intergenic 40.0%
GTATGTGTAGGCTTCACAAA+GGG + chr2.3:60361252-60361271 MS.gene74585:intron 40.0%
TCGTTACCCCTCAATTTGAA+GGG - chr2.3:60363315-60363334 None:intergenic 40.0%
TGGTGAGTTTAATAACGTGC+CGG + chr2.3:60360827-60360846 MS.gene74585:intron 40.0%
TGGTTGAGCAGATACTTTGT+TGG - chr2.3:60361301-60361320 None:intergenic 40.0%
! AAGGTTTAGTGGGTATGTGT+AGG + chr2.3:60361240-60361259 MS.gene74585:intron 40.0%
! CTAAACATGCACTGACCTTA+AGG - chr2.3:60360790-60360809 None:intergenic 40.0%
! TTGACAAAATTTGGTGGCAC+CGG - chr2.3:60360849-60360868 None:intergenic 40.0%
!! CTTTTCTAGAAAACGAGCCA+AGG - chr2.3:60363187-60363206 None:intergenic 40.0%
!! GTAATGGCATCGATTACTCT+TGG - chr2.3:60362976-60362995 None:intergenic 40.0%
!! TCGTTTTCTAGAAAAGCGCA+AGG + chr2.3:60363191-60363210 MS.gene74585:CDS 40.0%
!! TCTTTCAATCATGATGGGCA+AGG + chr2.3:60361926-60361945 MS.gene74585:CDS 40.0%
AAAGCCTAAACAGCACGGAA+AGG - chr2.3:60361465-60361484 None:intergenic 45.0%
AAAGGGTAGCATCAAACCTG+AGG - chr2.3:60361447-60361466 None:intergenic 45.0%
AAGCCTAAACAGCACGGAAA+GGG - chr2.3:60361464-60361483 None:intergenic 45.0%
AATCGATGCCATTACCATGC+TGG + chr2.3:60362981-60363000 MS.gene74585:CDS 45.0%
ACTACTCCATGGTACTGCTA+AGG + chr2.3:60360502-60360521 MS.gene74585:CDS 45.0%
AGTTGGGAAGAAAGCAGAGA+TGG - chr2.3:60362303-60362322 None:intergenic 45.0%
ATCGATTACTCTTGGAGGCT+CGG - chr2.3:60362968-60362987 None:intergenic 45.0%
CAATCAATAGAGGGAAGGGA+AGG - chr2.3:60361887-60361906 None:intergenic 45.0%
CACTCGGACCAATGTAATGT+TGG - chr2.3:60362478-60362497 None:intergenic 45.0%
CATAGCACAGACAGAACTTC+AGG + chr2.3:60362765-60362784 MS.gene74585:CDS 45.0%
CATTGGTCCGAGTGAAAAAC+TGG + chr2.3:60362484-60362503 MS.gene74585:intron 45.0%
CCGCATAGAAGATGGTAAGT+TGG - chr2.3:60362320-60362339 None:intergenic 45.0%
CCTTGTGGATGGGGAAAAAA+TGG + chr2.3:60362541-60362560 MS.gene74585:intron 45.0%
CGAATCAGAGACCTAGCTTA+AGG - chr2.3:60362519-60362538 None:intergenic 45.0%
CGATTCAGTCATTCCAGCAA+CGG - chr2.3:60362167-60362186 None:intergenic 45.0%
CGTTACCCCTCAATTTGAAG+GGG - chr2.3:60363314-60363333 None:intergenic 45.0%
CTCGTTACCCCTCAATTTGA+AGG - chr2.3:60363316-60363335 None:intergenic 45.0%
CTTCAAATTGAGGGGTAACG+AGG + chr2.3:60363314-60363333 MS.gene74585:intron 45.0%
GATGGATCATGCAAACACTC+TGG + chr2.3:60360551-60360570 MS.gene74585:intron 45.0%
GCATTGCTAACAGGAGTGAT+GGG - chr2.3:60362946-60362965 None:intergenic 45.0%
GGTATGTGTAGGCTTCACAA+AGG + chr2.3:60361251-60361270 MS.gene74585:intron 45.0%
GGTGGATCACACCAATAACT+TGG + chr2.3:60362633-60362652 MS.gene74585:intron 45.0%
TATGAAGCTGTCACTGCTTG+GGG + chr2.3:60362096-60362115 MS.gene74585:CDS 45.0%
TCCGAGTGAAAAACTGGACT+CGG + chr2.3:60362490-60362509 MS.gene74585:intron 45.0%
TCTGATCCCATAACATCTGC+TGG + chr2.3:60361369-60361388 MS.gene74585:CDS 45.0%
TGTAGGCTTCACAAAGGGTT+CGG + chr2.3:60361257-60361276 MS.gene74585:intron 45.0%
TTTGCGCCCCTTCAAATTGA+GGG + chr2.3:60363305-60363324 MS.gene74585:intron 45.0%
! AAGCTATCCTGTTGTTGGCT+AGG + chr2.3:60362719-60362738 MS.gene74585:CDS 45.0%
! AGACTAGTCGTCAGCAAAGT+CGG + chr2.3:60362612-60362631 MS.gene74585:intron 45.0%
! ATCCTGTTGTTGGCTAGGAA+TGG + chr2.3:60362724-60362743 MS.gene74585:CDS 45.0%
! CTTTTATGCAGCGATACCTC+AGG + chr2.3:60363149-60363168 MS.gene74585:intron 45.0%
! TCCGAGTCCAGTTTTTCACT+CGG - chr2.3:60362494-60362513 None:intergenic 45.0%
! TGCATTTGCAGGTTGACTCA+CGG - chr2.3:60362809-60362828 None:intergenic 45.0%
! TTCTAGAAAAGCGCAAGGAG+AGG + chr2.3:60363196-60363215 MS.gene74585:CDS 45.0%
! TTTTGCGCCCCTTCAAATTG+AGG + chr2.3:60363304-60363323 MS.gene74585:intron 45.0%
!! ATGGCATCGATTACTCTTGG+AGG - chr2.3:60362973-60362992 None:intergenic 45.0%
!! CTGACGACTAGTCTCTGTTT+GGG - chr2.3:60362606-60362625 None:intergenic 45.0%
!! GTTGGTGATCAAGGTCAAGT+TGG - chr2.3:60362460-60362479 None:intergenic 45.0%
!! TGATCAAGGTCAAGTTGGCA+AGG - chr2.3:60362455-60362474 None:intergenic 45.0%
!! TTCAATCATGATGGGCAAGG+CGG + chr2.3:60361929-60361948 MS.gene74585:CDS 45.0%
!!! CCATTTTTTCCCCATCCACA+AGG - chr2.3:60362544-60362563 None:intergenic 45.0%
AAGAGAGATACCTTGTCGGC+AGG - chr2.3:60362369-60362388 None:intergenic 50.0%
ACTCCATGGTACTGCTAAGG+AGG + chr2.3:60360505-60360524 MS.gene74585:CDS 50.0%
AGAGAGATACCTTGTCGGCA+GGG - chr2.3:60362368-60362387 None:intergenic 50.0%
ATGAAGCTGTCACTGCTTGG+GGG + chr2.3:60362097-60362116 MS.gene74585:CDS 50.0%
CAATCACCTGTTCCCACCAT+TGG + chr2.3:60362139-60362158 MS.gene74585:CDS 50.0%
CACCATTCCTAGCCAACAAC+AGG - chr2.3:60362729-60362748 None:intergenic 50.0%
CTAGTCTCTGTTTGGGAGCA+TGG - chr2.3:60362599-60362618 None:intergenic 50.0%
GGCACAAGCTATCCTGTTGT+TGG + chr2.3:60362714-60362733 MS.gene74585:intron 50.0%
GGCATTGCTAACAGGAGTGA+TGG - chr2.3:60362947-60362966 None:intergenic 50.0%
GGCTAGGAATGGTGTATCAG+TGG + chr2.3:60362735-60362754 MS.gene74585:CDS 50.0%
GTAGGCTTCACAAAGGGTTC+GGG + chr2.3:60361258-60361277 MS.gene74585:intron 50.0%
TATGGGACTTGGATGACCAG+AGG - chr2.3:60362839-60362858 None:intergenic 50.0%
TTGCGCCCCTTCAAATTGAG+GGG + chr2.3:60363306-60363325 MS.gene74585:intron 50.0%
! ATGGTGGGAACAGGTGATTG+AGG - chr2.3:60362139-60362158 None:intergenic 50.0%
! CGTTGATGAACTCATTCCCG+AGG + chr2.3:60362032-60362051 MS.gene74585:CDS 50.0%
! CTGATTCGAGCCTTGTGGAT+GGG + chr2.3:60362531-60362550 MS.gene74585:intron 50.0%
! GCATTTGCAGGTTGACTCAC+GGG - chr2.3:60362808-60362827 None:intergenic 50.0%
! TCTGATTCGAGCCTTGTGGA+TGG + chr2.3:60362530-60362549 MS.gene74585:intron 50.0%
! TGATTCGAGCCTTGTGGATG+GGG + chr2.3:60362532-60362551 MS.gene74585:intron 50.0%
!! CTACCCTTTCCGTGCTGTTT+AGG + chr2.3:60361458-60361477 MS.gene74585:intron 50.0%
!! GCTGACGACTAGTCTCTGTT+TGG - chr2.3:60362607-60362626 None:intergenic 50.0%
!! GGTGATTGAGGTGCTGTAAC+AGG - chr2.3:60362127-60362146 None:intergenic 50.0%
AAGGATGCTTTCCGAGCCTG+AGG - chr2.3:60363168-60363187 None:intergenic 55.0%
ACACGCGCTAAGCGCATGTT+TGG - chr2.3:60360886-60360905 None:intergenic 55.0%
ACATGCGCTTAGCGCGTGTT+TGG + chr2.3:60360887-60360906 MS.gene74585:intron 55.0%
ACCTGACGCCAGCATGGTAA+TGG - chr2.3:60362992-60363011 None:intergenic 55.0%
ACTCTCTGCTGCTGAACCTC+AGG + chr2.3:60361428-60361447 MS.gene74585:CDS 55.0%
ATGCAGCGATACCTCAGGCT+CGG + chr2.3:60363154-60363173 MS.gene74585:intron 55.0%
CAAAGGGTTCGGGTGCACAA+TGG + chr2.3:60361268-60361287 MS.gene74585:CDS 55.0%
CAGACAGAACTTCAGGCACC+TGG + chr2.3:60362772-60362791 MS.gene74585:CDS 55.0%
CATTCCAGCAACGGCACCAA+TGG - chr2.3:60362158-60362177 None:intergenic 55.0%
CCATGGTACTGCTAAGGAGG+AGG + chr2.3:60360508-60360527 MS.gene74585:CDS 55.0%
CCTCCTCCTTAGCAGTACCA+TGG - chr2.3:60360511-60360530 None:intergenic 55.0%
CTAGTCGTCAGCAAAGTCGG+TGG + chr2.3:60362615-60362634 MS.gene74585:intron 55.0%
GAACTTCAGGCACCTGGTGA+CGG + chr2.3:60362778-60362797 MS.gene74585:CDS 55.0%
GACGGTACCCGCATAGAAGA+TGG - chr2.3:60362328-60362347 None:intergenic 55.0%
GGACTCGGATCCCTTAAGCT+AGG + chr2.3:60362505-60362524 MS.gene74585:intron 55.0%
TCGCATACTGGTGCACAGTC+AGG + chr2.3:60362868-60362887 MS.gene74585:CDS 55.0%
! GGTCTCTGATTCGAGCCTTG+TGG + chr2.3:60362526-60362545 MS.gene74585:intron 55.0%
!! TCAGGCTCGGAAAGCATCCT+TGG + chr2.3:60363167-60363186 MS.gene74585:CDS 55.0%
ACTGGTGCACAGTCAGGCAG+TGG + chr2.3:60362874-60362893 MS.gene74585:CDS 60.0%
AGGATAGACCCCGCTGAAGG+TGG + chr2.3:60362569-60362588 MS.gene74585:intron 60.0%
ATGGCTGACCACCTTCAGCG+GGG - chr2.3:60362580-60362599 None:intergenic 60.0%
CAATGACGTCCCTGCCGACA+AGG + chr2.3:60362356-60362375 MS.gene74585:CDS 60.0%
CAGCATACCTGACGCCAGCA+TGG - chr2.3:60362998-60363017 None:intergenic 60.0%
CATGCTGGCGTCAGGTATGC+TGG + chr2.3:60362996-60363015 MS.gene74585:intron 60.0%
CATGGCTGACCACCTTCAGC+GGG - chr2.3:60362581-60362600 None:intergenic 60.0%
CCAGCAACGGCACCAATGGT+GGG - chr2.3:60362154-60362173 None:intergenic 60.0%
CTTGGATGACCAGAGGAGGC+TGG - chr2.3:60362832-60362851 None:intergenic 60.0%
GAGAGGATAGACCCCGCTGA+AGG + chr2.3:60362566-60362585 MS.gene74585:intron 60.0%
GAGGAGGCTGGTGCATTTGC+AGG - chr2.3:60362820-60362839 None:intergenic 60.0%
GCAAATGCACCAGCCTCCTC+TGG + chr2.3:60362820-60362839 MS.gene74585:CDS 60.0%
GCATGGCTGACCACCTTCAG+CGG - chr2.3:60362582-60362601 None:intergenic 60.0%
GCCATTACCATGCTGGCGTC+AGG + chr2.3:60362988-60363007 MS.gene74585:CDS 60.0%
GGAGGCTCGGCATTGCTAAC+AGG - chr2.3:60362955-60362974 None:intergenic 60.0%
GGGACTTGGATGACCAGAGG+AGG - chr2.3:60362836-60362855 None:intergenic 60.0%
TCCAGCAACGGCACCAATGG+TGG - chr2.3:60362155-60362174 None:intergenic 60.0%
! ACGGCACCAATGGTGGGAAC+AGG - chr2.3:60362148-60362167 None:intergenic 60.0%
!! CCCACCATTGGTGCCGTTGC+TGG + chr2.3:60362151-60362170 MS.gene74585:CDS 65.0%
CTCACGGGCACACCGTCACC+AGG - chr2.3:60362793-60362812 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 60360461 60363477 60360461 ID=MS.gene74585
chr2.3 mRNA 60360461 60363477 60360461 ID=MS.gene74585.t1;Parent=MS.gene74585
chr2.3 exon 60360461 60360545 60360461 ID=MS.gene74585.t1.exon1;Parent=MS.gene74585.t1
chr2.3 CDS 60360461 60360545 60360461 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60361262 60361449 60361262 ID=MS.gene74585.t1.exon2;Parent=MS.gene74585.t1
chr2.3 CDS 60361262 60361449 60361262 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60361923 60362185 60361923 ID=MS.gene74585.t1.exon3;Parent=MS.gene74585.t1
chr2.3 CDS 60361923 60362185 60361923 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60362290 60362377 60362290 ID=MS.gene74585.t1.exon4;Parent=MS.gene74585.t1
chr2.3 CDS 60362290 60362377 60362290 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60362715 60363009 60362715 ID=MS.gene74585.t1.exon5;Parent=MS.gene74585.t1
chr2.3 CDS 60362715 60363009 60362715 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60363160 60363217 60363160 ID=MS.gene74585.t1.exon6;Parent=MS.gene74585.t1
chr2.3 CDS 60363160 60363217 60363160 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
chr2.3 exon 60363417 60363477 60363417 ID=MS.gene74585.t1.exon7;Parent=MS.gene74585.t1
chr2.3 CDS 60363417 60363477 60363417 ID=cds.MS.gene74585.t1;Parent=MS.gene74585.t1
Gene Sequence

>MS.gene74585

ATGGTGAGAGATTTCATGGGTTTGAACTCAAAAGAAAAAGAACTACTCCATGGTACTGCTAAGGAGGAGGAAATCAACCATAATGGTGATGATGGATCATGCAAACACTCTGGCAAGTACTTTTCAATTTTCTTTGGATAACCGTTTTTTGTTTAGAGTACCATGCATGCAAACATTCATGAGTGTTTAATTTGATTGGTGGAACTAAGTAAGTTTTTGTTTCTTGTACTATAGATATTTAAAGTAGCATATGTATGTGTGCTAAATTTGTTATCAACTTCTTATCAATATTGTTTATTTTTTAAGAGATTCTTGTATAAGATATTCCTTAAGGTCAGTGCATGTTTAGATTGATGGTGAATTTGATGGTGAGTTTAATAACGTGCCGGTGCCACCAAATTTTGTCAAACTGATCGGTGATTCCAAACATGCGCTTAGCGCGTGTTTGGTTCTACCGTTACAAAAGTTGAATTTGAATGAATTGACTTTGTAAAATTGATTTTGATTAGAAGTGACTTGAATTGACATGTTTCTCATATAAAAATGGATTGAATGATAAATTTAATGCTAAGATCAATTGTAGGAGTCAAAGGTTACTAATTCCAATTTGAAGTTGAATCAATTCTAGAAACAAAATCAATCATATCCGAAAAGATCCAAACATTTCAAAATTAATTCTATACATCTAGAATCAAATTTGACTTCTCCAAAAGTGAAACCAAACATATACACTTAGTGGATGCCATATTGTGAATTTTTCAATCTATTTTTTATCCCTTAAGGTTTAGTGGGTATGTGTAGGCTTCACAAAGGGTTCGGGTGCACAATGGCCCTTTTCCAACAAAGTATCTGCTCAACCACATTTGATGTCTTTTGAGGTTTCTCAAGATGATAATACTAAGATCTTATCTGATCCCATAACATCTGCTGGTTTTATGACTCCAAATGCATTTGATTCTAGTAAAAAACTCTCTGCTGCTGAACCTCAGGTTTGATGCTACCCTTTCCGTGCTGTTTAGGCTTTAAACTTTGATAAACATCAGTGTAATTTTTTTTACATAGTTACACTGTCAACAAATCACAATCAATCATACGTAATTTTTGAAGAAATTATGATTAAAGTCAAAAGTTTTTAATGATCGACGATTATGACGGATTGACAGTGTGACTTTTTTTACATTGACGGTGTCTATGGATTAAATCGTTAACTTAAGTAAAATAAGGAATATTAGCTTATGAACAAGTAACATTTGGATGATCCAGTGTGTTCTAATCTGATTGGTATTCTAATTTTAAGTCAACTTTGTAGTATATCAATCAAATCCTCAAATTAAAATAAAATAAGTACTAGTTGTTTTGCAATATCTCATATCTTAACATACAAGATCTTACATTCAATACGTATCAGCTGATGTGTTTTTGCCCTTCCCTTCCCTCTATTGATTGTTTGTTATTACTATAGAAATCTTTCAATCATGATGGGCAAGGCGGCTTTAATTTTTCACGTACTCAGTATCCTGTACAACATGATGTGAAGATGTTTTCACTTTCCAATCAAACGAATTCAGTTTCGTTGATGAACTCATTCCCGAGGAATCATTTTGTAACTGCTGGTCAGAATATGAATGGTAATAATATGAAGCTGTCACTGCTTGGGGGAATACCTGTTACAGCACCTCAATCACCTGTTCCCACCATTGGTGCCGTTGCTGGAATGACTGAATCGTATTAATTTTACTTATTCTAATTTTATGATTGACTTTTAGGTGTACAGTGCACAAATTTGTTTGTATTTCATTTCTATAATAATTGATTTTATTTTGAAACAGCAATATGAAACCATCTCTGCTTTCTTCCCAACTTACCATCTTCTATGCGGGTACCGTCAATGTTTTCAATGACGTCCCTGCCGACAAGGTATCTCTCTTTGTTTATATTCCTTCTGCCTATAAATAAATACAAGGTAAAAAGGCCACAATTCATTTTTTTTTCCTTGCCAACTTGACCTTGATCACCAACATTACATTGGTCCGAGTGAAAAACTGGACTCGGATCCCTTAAGCTAGGTCTCTGATTCGAGCCTTGTGGATGGGGAAAAAATGGTTGAGAGGATAGACCCCGCTGAAGGTGGTCAGCCATGCTCCCAAACAGAGACTAGTCGTCAGCAAAGTCGGTGGATCACACCAATAACTTGGTTAACGAAAAAACTTGGCCTTGACCATAGTCAAGCTTAATTGTGGCACTATTTCTTCAGGCACAAGCTATCCTGTTGTTGGCTAGGAATGGTGTATCAGTGGCTTCTAACATAGCACAGACAGAACTTCAGGCACCTGGTGACGGTGTGCCCGTGAGTCAACCTGCAAATGCACCAGCCTCCTCTGGTCATCCAAGTCCCATATCTATTTCATCGCATACTGGTGCACAGTCAGGCAGTGGCTCAACTAGCACCAATGAATTTCTCGCAGTTAAAACAACTGGTGTTCCCATCACTCCTGTTAGCAATGCCGAGCCTCCAAGAGTAATCGATGCCATTACCATGCTGGCGTCAGGTATGCTGGAAGTACCATTTCTCATTATTGCAGTTAGATTATTTTGTTTATTGAAATCTTGTTTGGGGATATTTGTCTACATTTTACACATTGAAGAGAAACAAATCCGTTCTACACATTTAATAATTTCACTACCAATCTTTTATGCAGCGATACCTCAGGCTCGGAAAGCATCCTTGGCTCGTTTTCTAGAAAAGCGCAAGGAGAGGTTAGTATTGATGCCTTTTCTTTATAAAAGTAAAATACCATCCTAGATTAGATTTAAGGGCAACCATTTCTACACACATTACTTTTTTTTGCGCCCCTTCAAATTGAGGGGTAACGAGGATAATAACTTTGCTAATTTTACTAAAACTTAGCATTTGTAGTACTTTACATTACTTAAGCATTTAGTATTGTGTTTGCAGGGTGATGAATGCAGCACCATATAACATTAACAAGAAATCTGAAGAGTGTACTTCATCATAA
Protein sequence

>MS.gene74585.t1

MVRDFMGLNSKEKELLHGTAKEEEINHNGFTKGSGAQWPFSNKVSAQPHLMSFEVSQDDNTKILSDPITSAGFMTPNAFDSSKKLSAAEPQKSFNHDGQGGFNFSRTQYPVQHDVKMFSLSNQTNSVSLMNSFPRNHFVTAGQNMNGNNMKLSLLGGIPVTAPQSPVPTIGAVAGMTESNMKPSLLSSQLTIFYAGTVNVFNDVPADKAQAILLLARNGVSVASNIAQTELQAPGDGVPVSQPANAPASSGHPSPISISSHTGAQSGSGSTSTNEFLAVKTTGVPITPVSNAEPPRVIDAITMLASAIPQARKASLARFLEKRKERVMNAAPYNINKKSEECTSS