AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74612

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74612.t1 MTR_6g051425 30.075 266 177 3 97 353 20 285 1.38e-37 144
MS.gene74612.t1 MTR_1g070355 29.682 283 179 10 53 323 34 308 2.84e-21 99.0
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 55 158 3.08e-15 76.3
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 55 158 3.20e-15 76.3
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 75 178 4.57e-15 76.3
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 75 178 4.64e-15 76.3
MS.gene74612.t1 MTR_5g076070 26.408 284 174 10 52 318 17 282 4.71e-15 76.6
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 73 176 4.93e-15 76.3
MS.gene74612.t1 MTR_4g067290 36.538 104 63 1 93 193 115 218 5.91e-15 76.6
MS.gene74612.t1 MTR_2g098920 34.014 147 90 3 87 228 75 219 9.30e-15 75.5
MS.gene74612.t1 MTR_0558s0020 25.843 267 148 10 100 329 42 295 3.64e-14 74.3
MS.gene74612.t1 MTR_7g446520 25.714 245 163 5 92 332 86 315 7.86e-14 73.9
MS.gene74612.t1 MTR_8g446020 28.649 185 110 6 157 329 81 255 8.12e-13 69.7
MS.gene74612.t1 MTR_2g098880 29.032 155 94 3 43 191 20 164 1.50e-12 68.6
MS.gene74612.t1 MTR_2g098880 29.032 155 94 3 43 191 32 176 1.83e-12 68.6
MS.gene74612.t1 MTR_7g446510 29.213 178 114 3 169 338 156 329 1.49e-11 67.0
MS.gene74612.t1 MTR_7g446480 28.736 174 120 2 169 338 141 314 4.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74612.t1 AT1G80010 53.528 652 286 7 73 712 74 720 0.0 696
MS.gene74612.t1 AT1G52520 50.147 678 297 13 57 713 42 699 0.0 643
MS.gene74612.t1 AT4G15090 33.387 620 398 6 93 706 50 660 3.85e-120 380
MS.gene74612.t1 AT4G15090 33.387 620 398 6 93 706 50 660 3.85e-120 380
MS.gene74612.t1 AT4G15090 33.387 620 398 6 93 706 50 660 3.85e-120 380
MS.gene74612.t1 AT4G15090 33.387 620 398 6 93 706 50 660 3.85e-120 380
MS.gene74612.t1 AT4G15090 33.387 620 398 6 93 706 50 660 3.85e-120 380
MS.gene74612.t1 AT1G76320 34.138 621 383 9 98 712 1 601 5.31e-112 354
MS.gene74612.t1 AT1G76320 34.138 621 383 9 98 712 1 601 1.45e-111 355
MS.gene74612.t1 AT1G76320 34.138 621 383 9 98 712 1 601 1.45e-111 355
MS.gene74612.t1 AT1G76320 34.138 621 383 9 98 712 1 601 1.47e-111 355
MS.gene74612.t1 AT3G22170 29.250 653 412 7 93 727 69 689 2.35e-104 338
MS.gene74612.t1 AT3G22170 29.250 653 412 7 93 727 69 689 2.35e-104 338
MS.gene74612.t1 AT4G38180 32.982 570 366 8 93 653 72 634 1.82e-103 335
MS.gene74612.t1 AT2G27110 29.586 676 417 15 45 713 16 639 3.58e-92 306
MS.gene74612.t1 AT2G27110 29.586 676 417 15 45 713 16 639 3.58e-92 306
MS.gene74612.t1 AT5G18960 29.430 632 402 9 79 709 196 784 1.32e-89 298
MS.gene74612.t1 AT5G18960 30.159 126 79 1 93 218 42 158 1.00e-12 72.4
MS.gene74612.t1 AT5G18960 29.430 632 402 9 79 709 196 784 1.32e-89 298
MS.gene74612.t1 AT5G18960 30.159 126 79 1 93 218 42 158 1.00e-12 72.4
MS.gene74612.t1 AT3G06250 28.834 652 408 10 67 709 156 760 1.33e-87 292
MS.gene74612.t1 AT3G06250 30.405 148 91 3 93 240 27 162 7.80e-13 72.4
MS.gene74612.t1 AT3G06250 28.834 652 408 10 67 709 156 760 1.33e-87 292
MS.gene74612.t1 AT3G06250 30.405 148 91 3 93 240 27 162 7.80e-13 72.4
MS.gene74612.t1 AT3G06250 28.834 652 408 10 67 709 156 760 1.33e-87 292
MS.gene74612.t1 AT3G06250 30.405 148 91 3 93 240 27 162 7.80e-13 72.4
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 1.74e-87 291
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 2.06e-87 292
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 2.06e-87 292
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 2.16e-87 292
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 2.16e-87 292
MS.gene74612.t1 AT2G32250 31.655 556 332 6 97 650 41 550 2.16e-87 292
MS.gene74612.t1 AT2G32250 31.532 555 332 6 98 650 1 509 9.84e-87 290
MS.gene74612.t1 AT2G32250 31.532 555 332 6 98 650 1 509 1.24e-86 290
MS.gene74612.t1 AT2G27110 32.645 484 305 7 237 713 25 494 1.61e-84 282
MS.gene74612.t1 AT4G19990 30.236 592 350 10 96 667 23 571 1.81e-83 281
MS.gene74612.t1 AT4G19990 30.236 592 350 10 96 667 23 571 1.94e-83 280
MS.gene74612.t1 AT4G19990 30.236 592 350 10 96 667 23 571 1.94e-83 280
MS.gene74612.t1 AT4G19990 29.853 613 367 10 75 667 16 585 3.18e-83 280
MS.gene74612.t1 AT4G19990 30.236 592 350 10 96 667 23 571 5.37e-83 280
MS.gene74612.t1 AT4G19990 30.236 592 350 10 96 667 23 571 5.37e-83 280
MS.gene74612.t1 AT4G19990 31.073 531 313 10 145 667 29 514 4.93e-76 259
MS.gene74612.t1 AT4G38170 30.998 471 296 9 265 724 19 471 1.12e-71 244
MS.gene74612.t1 AT1G10240 25.389 579 392 13 92 650 46 604 6.67e-53 195
MS.gene74612.t1 AT1G10240 25.389 579 392 13 92 650 46 604 6.67e-53 195
MS.gene74612.t1 AT5G28530 26.500 600 385 18 94 664 55 627 3.47e-46 176
MS.gene74612.t1 AT5G28530 26.167 600 383 18 94 664 55 623 2.80e-45 173
MS.gene74612.t1 AT5G28530 26.167 600 383 18 94 664 55 623 2.80e-45 173
MS.gene74612.t1 AT5G28530 26.488 336 217 9 336 664 252 564 2.81e-24 108
MS.gene74612.t1 AT5G28530 25.893 336 215 9 336 664 252 560 1.89e-23 105
MS.gene74612.t1 AT3G59470 37.037 108 65 1 93 197 69 176 4.22e-15 76.3
MS.gene74612.t1 AT3G59470 37.037 108 65 1 93 197 69 176 4.22e-15 76.3
MS.gene74612.t1 AT3G59470 37.037 108 65 1 93 197 81 188 4.53e-15 76.6

Find 153 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAACCAAATAATGATTT+TGG 0.088603 2.3:+60699867 None:intergenic
GACAATTGAGGAACATATTT+TGG 0.117184 2.3:-60701068 MS.gene74612:CDS
GATATCCCAAAATCATTATT+TGG 0.175601 2.3:-60699873 MS.gene74612:CDS
TGGTTGTCTGGTGCAGTTTC+AGG 0.204396 2.3:-60700713 MS.gene74612:CDS
AATAGCTATGCCAGGGAAAT+TGG 0.208039 2.3:-60701609 MS.gene74612:CDS
AGGGCTGCATATAGTTATTT+TGG 0.210404 2.3:-60701030 MS.gene74612:CDS
AAAATCATTATTTGGTTTCT+TGG 0.223441 2.3:-60699865 MS.gene74612:CDS
TTTGGATTGATTCTAGTTCT+AGG 0.231462 2.3:-60701050 MS.gene74612:CDS
AACTGAAGCTCTTGATGATT+TGG 0.234723 2.3:-60700383 MS.gene74612:CDS
CGGTTTGTAACAATAGTTTC+TGG 0.236513 2.3:+60700823 None:intergenic
AATTAGGACTCACCAGTTTA+TGG 0.247549 2.3:+60701121 None:intergenic
ATTTATAGAGTCTTTGAATA+AGG 0.281326 2.3:-60699837 MS.gene74612:intron
TAGATGAATTTGAAAGAGAT+TGG 0.315956 2.3:-60700636 MS.gene74612:CDS
AGTCGGCTCAACCTTTCTTT+TGG 0.323275 2.3:+60701314 None:intergenic
TGTGACAATCCTTTATCATT+TGG 0.329411 2.3:+60703263 None:intergenic
ACTGTAGATGCTTCCGGTTA+TGG 0.346656 2.3:-60701249 MS.gene74612:CDS
CTCTACTATTGCACATTCTA+TGG 0.347062 2.3:+60700792 None:intergenic
AAATGATCAAAACACTGAAC+TGG 0.347410 2.3:+60699932 None:intergenic
TTACCGTGATGTCGAGATTA+GGG 0.349365 2.3:+60703237 None:intergenic
CTGTAGATGCTTCCGGTTAT+GGG 0.351570 2.3:-60701248 MS.gene74612:CDS
AGTCAATTGTTTGAGATTGA+TGG 0.375923 2.3:-60701768 MS.gene74612:CDS
CTCTGTCCTCGTGCAAGCTT+CGG 0.376248 2.3:+60700569 None:intergenic
GCACGAGGACAGAGAGCTTT+GGG 0.378976 2.3:-60700560 MS.gene74612:CDS
CTTCTCTTTACTGTTACGTT+TGG 0.380146 2.3:+60701557 None:intergenic
TTTACCGTGATGTCGAGATT+AGG 0.383499 2.3:+60703236 None:intergenic
TTACTTCTGCCACCATAAAC+TGG 0.384916 2.3:-60701133 MS.gene74612:CDS
TTGGATTGATTCTAGTTCTA+GGG 0.391970 2.3:-60701049 MS.gene74612:CDS
AAATGCCTATCATTTCTTAC+CGG 0.393224 2.3:+60700166 None:intergenic
TGAGAGTAGTGGAATCTAAT+AGG 0.393466 2.3:-60701431 MS.gene74612:CDS
ATATTTCGAGCAGGGTTTGA+TGG 0.395255 2.3:+60699559 None:intergenic
TTATTGCATAATGTTGGGTC+AGG 0.399471 2.3:+60700608 None:intergenic
TGCACGAGGACAGAGAGCTT+TGG 0.400964 2.3:-60700561 MS.gene74612:CDS
GATTTCTTATTGCATAATGT+TGG 0.401141 2.3:+60700602 None:intergenic
GATGGTGGCGAACATGAAAA+TGG 0.403685 2.3:-60701750 MS.gene74612:CDS
TGGAATTTCTAATTACGAAA+AGG 0.404269 2.3:-60700506 MS.gene74612:CDS
TCAAACTCCACTCCCTCAAC+AGG 0.406663 2.3:+60701660 None:intergenic
CTTGCAATAGACAAATTCTA+TGG 0.410394 2.3:+60700752 None:intergenic
AGAATTTGAGCTCCCATAAC+CGG 0.414377 2.3:+60701236 None:intergenic
GTGTGTTCTCCAAATGATAA+AGG 0.415956 2.3:-60703272 MS.gene74612:CDS
ATTTCTTATTGCATAATGTT+GGG 0.422728 2.3:+60700603 None:intergenic
ATGCCAACTGTAGATGCTTC+CGG 0.429407 2.3:-60701255 MS.gene74612:CDS
CTATTCGTGTTAAATCTTCG+TGG 0.432290 2.3:-60701581 MS.gene74612:CDS
GGAATTTCTAATTACGAAAA+GGG 0.436213 2.3:-60700505 MS.gene74612:CDS
AATTTGTGAGACGCTGAAAC+AGG 0.436325 2.3:+60700243 None:intergenic
AATGTGTCCTTGGAGTAAAC+TGG 0.441443 2.3:+60700535 None:intergenic
GGTTTGTAACAATAGTTTCT+GGG 0.441868 2.3:+60700824 None:intergenic
CTCTTTCAGGATCAAATGAA+TGG 0.442050 2.3:+60701376 None:intergenic
ACTTCAAAGGCTACCTATGC+AGG 0.442185 2.3:-60700087 MS.gene74612:CDS
ATTCCACTAGTTGCATTTGT+TGG 0.443557 2.3:-60700961 MS.gene74612:CDS
AGATGAATTTGAAAGAGATT+GGG 0.444699 2.3:-60700635 MS.gene74612:CDS
GCAGATCATCCGTGGTTGTC+TGG 0.446361 2.3:-60700725 MS.gene74612:CDS
CTGCATTGTCGGTCATGATT+CGG 0.448661 2.3:+60703294 None:intergenic
CTCCACTCCCTCAACAGGTT+GGG 0.449152 2.3:+60701665 None:intergenic
TGTAATTGCTTTAACTTCAA+AGG 0.458366 2.3:-60700100 MS.gene74612:CDS
GATGCAGGTTGCAGCTGTTA+AGG 0.463308 2.3:-60699582 MS.gene74612:intron
AGAACATCAAGGGGAAGAAC+CGG 0.463849 2.3:-60700185 MS.gene74612:CDS
TGGAGAACACACTGCATTGT+CGG 0.473508 2.3:+60703283 None:intergenic
TAGTGGAATCTAATAGGTGG+AGG 0.476847 2.3:-60701425 MS.gene74612:CDS
ATAGCTATTATAATAGTTGT+AGG 0.483777 2.3:+60701623 None:intergenic
CGCCCAACCTGTTGAGGGAG+TGG 0.485119 2.3:-60701667 MS.gene74612:CDS
TGCTCGAAATATGTGTTTGG+TGG 0.487448 2.3:-60699548 MS.gene74612:CDS
CCCTGCTCGAAATATGTGTT+TGG 0.491484 2.3:-60699551 MS.gene74612:CDS
ACATCATCACCACCTCATCT+TGG 0.493280 2.3:+60700269 None:intergenic
GTCTTGTGTTGTAGTTGTGA+AGG 0.496440 2.3:-60701525 MS.gene74612:CDS
TAGAGAGTTGCAACTCATAG+TGG 0.499235 2.3:+60700323 None:intergenic
CGTAGGAAAGAAACAAGAAC+AGG 0.499540 2.3:-60701474 MS.gene74612:CDS
AGACATTTGAAACGTATACT+TGG 0.502054 2.3:-60700885 MS.gene74612:CDS
ATCCTTAATTACAATGGTGT+GGG 0.502114 2.3:-60700052 MS.gene74612:CDS
ACTATTATAATAGCTATGCC+AGG 0.505200 2.3:-60701617 MS.gene74612:CDS
TTATCATAATTCATGTATGT+AGG 0.507183 2.3:+60699524 None:intergenic
AAGAACCGGTAAGAAATGAT+AGG 0.508332 2.3:-60700171 MS.gene74612:CDS
ACCAAACACATATTTCGAGC+AGG 0.509508 2.3:+60699550 None:intergenic
CACATAGGAGGATGGACAGT+GGG 0.512891 2.3:-60701338 MS.gene74612:CDS
CAAATTCTATGGTGAGCCCT+CGG 0.514324 2.3:+60700763 None:intergenic
ATTACAAATGCAACGTACTT+CGG 0.514793 2.3:+60700117 None:intergenic
TACTATTTCCAGATAGATGA+GGG 0.522528 2.3:-60701855 MS.gene74612:intron
TAAGCAACTACTTGAGGTTG+AGG 0.526880 2.3:-60701820 MS.gene74612:CDS
GGACAGTGGGGCCAAAAGAA+AGG 0.531952 2.3:-60701325 MS.gene74612:CDS
ATACTTGGTTATTTAGAGCA+TGG 0.532023 2.3:-60700870 MS.gene74612:CDS
GGGAGCTCAAATTCTAATGA+AGG 0.533763 2.3:-60701228 MS.gene74612:CDS
ATCAAAACACTGAACTGGGT+TGG 0.536498 2.3:+60699937 None:intergenic
AGACTACGCCCAACCTGTTG+AGG 0.540785 2.3:-60701673 MS.gene74612:CDS
AAATTCTATGGTGAGCCCTC+GGG 0.542802 2.3:+60700764 None:intergenic
CAGAACTCATGTGAACTCTC+TGG 0.546800 2.3:-60703317 MS.gene74612:CDS
TATCCTTAATTACAATGGTG+TGG 0.549164 2.3:-60700053 MS.gene74612:CDS
ACTCCACTCCCTCAACAGGT+TGG 0.553844 2.3:+60701664 None:intergenic
GACTACGCCCAACCTGTTGA+GGG 0.556672 2.3:-60701672 MS.gene74612:CDS
GTGATGGATCTGAATGATGA+TGG 0.557426 2.3:-60701090 MS.gene74612:CDS
AATAGCAAATCCAATTTCCC+TGG 0.562021 2.3:+60701599 None:intergenic
CCAAACACATATTTCGAGCA+GGG 0.562427 2.3:+60699551 None:intergenic
TATACAAGGCATGCCTGCAT+AGG 0.564010 2.3:+60700074 None:intergenic
GGAAGATGAGGCATTTCAGA+TGG 0.566330 2.3:-60700692 MS.gene74612:CDS
TATTGCATAATGTTGGGTCA+GGG 0.567535 2.3:+60700609 None:intergenic
ACATGGTAAAAGAACATCAA+GGG 0.568150 2.3:-60700195 MS.gene74612:CDS
CGAGTCTATAGTGACATACA+TGG 0.575473 2.3:-60700212 MS.gene74612:CDS
TTGTATATCCTTAATTACAA+TGG 0.575751 2.3:-60700058 MS.gene74612:CDS
CACATTCTATGGTCTTACAT+CGG 0.577150 2.3:+60700803 None:intergenic
CTCTAAATCACATAGGAGGA+TGG 0.578313 2.3:-60701346 MS.gene74612:CDS
GTTTGTAACAATAGTTTCTG+GGG 0.579296 2.3:+60700825 None:intergenic
TTTGTAACAATAGTTTCTGG+GGG 0.582883 2.3:+60700826 None:intergenic
TTACTATTTCCAGATAGATG+AGG 0.583204 2.3:-60701856 MS.gene74612:intron
GTGGCGAACATGAAAATGGA+AGG 0.585092 2.3:-60701746 MS.gene74612:CDS
ACTACTTGAGGTTGAGGACA+AGG 0.585298 2.3:-60701814 MS.gene74612:CDS
AATGATCAAAACACTGAACT+GGG 0.585970 2.3:+60699933 None:intergenic
ACAAAGTAAGCAACTACTTG+AGG 0.589391 2.3:-60701826 MS.gene74612:CDS
TCCTTAATTACAATGGTGTG+GGG 0.589480 2.3:-60700051 MS.gene74612:CDS
ATAAAGAAGCTAATAGTCGT+AGG 0.594056 2.3:-60701491 MS.gene74612:CDS
TACATGGTAAAAGAACATCA+AGG 0.596297 2.3:-60700196 MS.gene74612:CDS
ACACCAACAAATGCAACTAG+TGG 0.596327 2.3:+60700958 None:intergenic
TTGGGCTCCAGTTTACTCCA+AGG 0.596913 2.3:-60700542 MS.gene74612:CDS
AAAACTCTAAATCACATAGG+AGG 0.599156 2.3:-60701350 MS.gene74612:CDS
GCTCGAAATATGTGTTTGGT+GGG 0.599382 2.3:-60699547 MS.gene74612:CDS
CGACTCTAGATGTTGAAGTA+CGG 0.600367 2.3:-60701296 MS.gene74612:CDS
GGAATCTAATAGGTGGAGGG+TGG 0.600823 2.3:-60701421 MS.gene74612:CDS
CTTGACAAAATATAATGACA+TGG 0.602201 2.3:+60700022 None:intergenic
CAAGTCTTGCAGATCATCCG+TGG 0.605006 2.3:-60700733 MS.gene74612:CDS
ATTCAATAAGTTCCAAGATG+AGG 0.606495 2.3:-60700281 MS.gene74612:CDS
CATAGAATGTGCAATAGTAG+AGG 0.608377 2.3:-60700791 MS.gene74612:CDS
GGTATGCAAGTAGTTGAAGA+AGG 0.610848 2.3:-60699899 MS.gene74612:CDS
TAACCGGAAGCATCTACAGT+TGG 0.612183 2.3:+60701252 None:intergenic
ACTATGACTGTCAACTCCCG+TGG 0.616006 2.3:+60701716 None:intergenic
GAGTAGTGGAATCTAATAGG+TGG 0.616272 2.3:-60701428 MS.gene74612:CDS
CAATAAGTTCCAAGATGAGG+TGG 0.616780 2.3:-60700278 MS.gene74612:CDS
AGCTCAAATTCTAATGAAGG+AGG 0.617343 2.3:-60701225 MS.gene74612:CDS
CGCAAAACTCTAAATCACAT+AGG 0.623536 2.3:-60701353 MS.gene74612:CDS
AATGGAAGGGAGGAAACCAC+GGG 0.626247 2.3:-60701732 MS.gene74612:CDS
GGTGCAGTTTCAGGAAGATG+AGG 0.635119 2.3:-60700704 MS.gene74612:CDS
AAATGGAAGGGAGGAAACCA+CGG 0.638573 2.3:-60701733 MS.gene74612:CDS
AAGCCCTAATCTCGACATCA+CGG 0.640632 2.3:-60703240 MS.gene74612:intron
CCTTAATTACAATGGTGTGG+GGG 0.643743 2.3:-60700050 MS.gene74612:CDS
ATTGTAATTAAGGATATACA+AGG 0.644132 2.3:+60700060 None:intergenic
CATGGTAAAAGAACATCAAG+GGG 0.645016 2.3:-60700194 MS.gene74612:CDS
CGTACTTCAACATCTAGAGT+CGG 0.648024 2.3:+60701297 None:intergenic
CTATTATAATAGCTATGCCA+GGG 0.648310 2.3:-60701616 MS.gene74612:CDS
TTTGTTGGTGTTAATCACCA+TGG 0.653331 2.3:-60700946 MS.gene74612:CDS
ACATTGTCAGAGCTGAGATG+TGG 0.654932 2.3:+60699968 None:intergenic
TAGGACTCACCAGTTTATGG+TGG 0.657453 2.3:+60701124 None:intergenic
CAATTGTTTGAGATTGATGG+TGG 0.658357 2.3:-60701765 MS.gene74612:CDS
AGTGGAATCTAATAGGTGGA+GGG 0.661358 2.3:-60701424 MS.gene74612:CDS
TTTGATCATTTGTACAAACG+TGG 0.664936 2.3:-60699920 MS.gene74612:CDS
AATGATTAGACTGAGAGTAG+TGG 0.669263 2.3:-60701442 MS.gene74612:CDS
TGGCGAACATGAAAATGGAA+GGG 0.678993 2.3:-60701745 MS.gene74612:CDS
CGAACATGAAAATGGAAGGG+AGG 0.681120 2.3:-60701742 MS.gene74612:CDS
TTGTAACAATAGTTTCTGGG+GGG 0.684006 2.3:+60700827 None:intergenic
CTACTTGAGGTTGAGGACAA+GGG 0.687690 2.3:-60701813 MS.gene74612:CDS
AAAGCTCCGAAGCTTGCACG+AGG 0.688357 2.3:-60700575 MS.gene74612:CDS
CTAGCAAAACAGATTGACCA+TGG 0.689075 2.3:+60700929 None:intergenic
GAAACTGCACCAGACAACCA+CGG 0.692031 2.3:+60700716 None:intergenic
TCACATAGGAGGATGGACAG+TGG 0.701404 2.3:-60701339 MS.gene74612:CDS
ACTATTTCCAGATAGATGAG+GGG 0.701880 2.3:-60701854 MS.gene74612:intron
ACATAGGAGGATGGACAGTG+GGG 0.705660 2.3:-60701337 MS.gene74612:CDS
CGTAACAGTAAAGAGAAGCG+TGG 0.709182 2.3:-60701552 MS.gene74612:CDS
TGAATGATGATGGACAATTG+AGG 0.749302 2.3:-60701080 MS.gene74612:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTTTATTGATTAAC+AGG - chr2.3:60700174-60700193 MS.gene74612:CDS 10.0%
!!! ATATTGTTAACAATTTTAAA+GGG + chr2.3:60700435-60700454 None:intergenic 10.0%
!!! TATATTGTTAACAATTTTAA+AGG + chr2.3:60700436-60700455 None:intergenic 10.0%
!! AGTAATATAAAATTTCATGT+TGG - chr2.3:60700840-60700859 MS.gene74612:CDS 15.0%
!! ATAATCAGTGTTATAAATAA+AGG + chr2.3:60700629-60700648 None:intergenic 15.0%
!! ATTATTTCACATAATTACTT+TGG - chr2.3:60700151-60700170 MS.gene74612:CDS 15.0%
!! GTAATATAAAATTTCATGTT+GGG - chr2.3:60700841-60700860 MS.gene74612:CDS 15.0%
!! TGAAACTTATATTGTTAATA+TGG + chr2.3:60700496-60700515 None:intergenic 15.0%
!!! TATTGTTAACAATTTTAAAG+GGG + chr2.3:60700434-60700453 None:intergenic 15.0%
!!! TGAAATTTTATATTACTTAC+AGG + chr2.3:60700837-60700856 None:intergenic 15.0%
!! AAGATATAATGATAGTATAG+TGG - chr2.3:60700746-60700765 MS.gene74612:CDS 20.0%
!! AAGATTAAAAATTCAGAGTT+TGG + chr2.3:60699778-60699797 None:intergenic 20.0%
!! AGATATAATGATAGTATAGT+GGG - chr2.3:60700747-60700766 MS.gene74612:CDS 20.0%
!! ATAGCTATTATAATAGTTGT+AGG + chr2.3:60701208-60701227 None:intergenic 20.0%
!! ATTGTAATTAAGGATATACA+AGG + chr2.3:60702771-60702790 None:intergenic 20.0%
!! ATTTATAGAGTCTTTGAATA+AGG - chr2.3:60702991-60703010 MS.gene74612:intron 20.0%
!! ATTTCTTATTGCATAATGTT+GGG + chr2.3:60702228-60702247 None:intergenic 20.0%
!! CATGGTTACTTAAAATAAAA+GGG - chr2.3:60700032-60700051 MS.gene74612:CDS 20.0%
!! GTCATAACATAATATAATAG+CGG - chr2.3:60700557-60700576 MS.gene74612:CDS 20.0%
!! TCAAAGAGAAAAAAAATTGT+GGG - chr2.3:60700361-60700380 MS.gene74612:CDS 20.0%
!! TCATGGTTACTTAAAATAAA+AGG - chr2.3:60700031-60700050 MS.gene74612:CDS 20.0%
!! TGTTATGACATTGATATTAT+TGG + chr2.3:60700546-60700565 None:intergenic 20.0%
!! TGTTTGCAAATAAATAGTTT+GGG + chr2.3:60699711-60699730 None:intergenic 20.0%
!! TTATCATAATTCATGTATGT+AGG + chr2.3:60703307-60703326 None:intergenic 20.0%
!! TTCAAAGAGAAAAAAAATTG+TGG - chr2.3:60700360-60700379 MS.gene74612:CDS 20.0%
!! TTGTATATCCTTAATTACAA+TGG - chr2.3:60702770-60702789 MS.gene74612:intron 20.0%
!! TTGTTTGCAAATAAATAGTT+TGG + chr2.3:60699712-60699731 None:intergenic 20.0%
!!! AAAAATTTGAAGTGATAAAC+AGG + chr2.3:60700081-60700100 None:intergenic 20.0%
!!! AAAATCATTATTTGGTTTCT+TGG - chr2.3:60702963-60702982 MS.gene74612:intron 20.0%
!!! AAGAAACCAAATAATGATTT+TGG + chr2.3:60702964-60702983 None:intergenic 20.0%
!!! AGAAACCAAATAATGATTTT+GGG + chr2.3:60702963-60702982 None:intergenic 20.0%
!!! AGAATAATTGTTTTTGAAGA+AGG - chr2.3:60700200-60700219 MS.gene74612:CDS 20.0%
!!! CAAATTCATCTAATGTTTTA+GGG + chr2.3:60702184-60702203 None:intergenic 20.0%
!!! GAATAATTGTTTTTGAAGAA+GGG - chr2.3:60700201-60700220 MS.gene74612:CDS 20.0%
!!! TCAAATTCATCTAATGTTTT+AGG + chr2.3:60702185-60702204 None:intergenic 20.0%
!!! TTTTAATGATAACACTGATA+GGG - chr2.3:60700387-60700406 MS.gene74612:CDS 20.0%
!!! TTTTTAATGATAACACTGAT+AGG - chr2.3:60700386-60700405 MS.gene74612:CDS 20.0%
! AACAATATAACCATGATTCA+AGG - chr2.3:60700447-60700466 MS.gene74612:CDS 25.0%
! AGAACAATTTATGTATCAGA+AGG + chr2.3:60703108-60703127 None:intergenic 25.0%
! AGATGAATTTGAAAGAGATT+GGG - chr2.3:60702193-60702212 MS.gene74612:intron 25.0%
! CTTGACAAAATATAATGACA+TGG + chr2.3:60702809-60702828 None:intergenic 25.0%
! GATATCCCAAAATCATTATT+TGG - chr2.3:60702955-60702974 MS.gene74612:intron 25.0%
! GATTTCTTATTGCATAATGT+TGG + chr2.3:60702229-60702248 None:intergenic 25.0%
! GGAATTTCTAATTACGAAAA+GGG - chr2.3:60702323-60702342 MS.gene74612:intron 25.0%
! GTGAAATAATCAATGTCATT+AGG + chr2.3:60700141-60700160 None:intergenic 25.0%
! TAGATGAATTTGAAAGAGAT+TGG - chr2.3:60702192-60702211 MS.gene74612:intron 25.0%
! TCCATCACATAGAAAAAATT+AGG + chr2.3:60701726-60701745 None:intergenic 25.0%
! TGGAATTTCTAATTACGAAA+AGG - chr2.3:60702322-60702341 MS.gene74612:intron 25.0%
! TTACTTAAAATAAAAGGGAG+TGG - chr2.3:60700037-60700056 MS.gene74612:CDS 25.0%
! TTTAATGATAACACTGATAG+GGG - chr2.3:60700388-60700407 MS.gene74612:CDS 25.0%
!! AAATAAATAGTTTGGGTTAG+AGG + chr2.3:60699704-60699723 None:intergenic 25.0%
!! ATAGAATGTGAATGTCTTTT+TGG - chr2.3:60703175-60703194 MS.gene74612:intron 25.0%
!! ATTATATTTTGTCAAGATGG+AGG - chr2.3:60702813-60702832 MS.gene74612:intron 25.0%
!! GTCATTATATTTTGTCAAGA+TGG - chr2.3:60702810-60702829 MS.gene74612:intron 25.0%
!! TGTAATTGCTTTAACTTCAA+AGG - chr2.3:60702728-60702747 MS.gene74612:intron 25.0%
!! TTTTGTTGAGAAAAGAAAAG+AGG - chr2.3:60699739-60699758 MS.gene74612:intron 25.0%
!!! ATCTATGATTCCCTTTTTTA+AGG - chr2.3:60702349-60702368 MS.gene74612:intron 25.0%
!!! TCCTAATTTTTTCTATGTGA+TGG - chr2.3:60701722-60701741 MS.gene74612:CDS 25.0%
!!! TCTATGATTCCCTTTTTTAA+GGG - chr2.3:60702350-60702369 MS.gene74612:intron 25.0%
!!! TCTGTTTTTTAAGTTCAAAG+TGG - chr2.3:60699917-60699936 MS.gene74612:CDS 25.0%
AAAACTCTAAATCACATAGG+AGG - chr2.3:60701478-60701497 MS.gene74612:CDS 30.0%
AAACAAGTCATTAAGTTCCA+TGG + chr2.3:60699630-60699649 None:intergenic 30.0%
AAATGATCAAAACACTGAAC+TGG + chr2.3:60702899-60702918 None:intergenic 30.0%
AAATGCCTATCATTTCTTAC+CGG + chr2.3:60702665-60702684 None:intergenic 30.0%
AATATAACCATGATTCAAGG+CGG - chr2.3:60700450-60700469 MS.gene74612:CDS 30.0%
AATGATCAAAACACTGAACT+GGG + chr2.3:60702898-60702917 None:intergenic 30.0%
AATTTATGTATCAGAAGGCA+TGG + chr2.3:60703103-60703122 None:intergenic 30.0%
ACATGGTAAAAGAACATCAA+GGG - chr2.3:60702633-60702652 MS.gene74612:intron 30.0%
ACTATTATAATAGCTATGCC+AGG - chr2.3:60701211-60701230 MS.gene74612:CDS 30.0%
ATAAAGAAGCTAATAGTCGT+AGG - chr2.3:60701337-60701356 MS.gene74612:CDS 30.0%
ATCCTTAATTACAATGGTGT+GGG - chr2.3:60702776-60702795 MS.gene74612:intron 30.0%
ATGCTAATATGTTGTGCATA+AGG - chr2.3:60703057-60703076 MS.gene74612:intron 30.0%
ATTACAAATGCAACGTACTT+CGG + chr2.3:60702714-60702733 None:intergenic 30.0%
ATTCAATAAGTTCCAAGATG+AGG - chr2.3:60702547-60702566 MS.gene74612:intron 30.0%
CTATTATAATAGCTATGCCA+GGG - chr2.3:60701212-60701231 MS.gene74612:CDS 30.0%
CTTGCAATAGACAAATTCTA+TGG + chr2.3:60702079-60702098 None:intergenic 30.0%
GGTTTGTAACAATAGTTTCT+GGG + chr2.3:60702007-60702026 None:intergenic 30.0%
GTAAATCTTCATCTTAACCA+TGG - chr2.3:60699610-60699629 MS.gene74612:intron 30.0%
GTTTGTAACAATAGTTTCTG+GGG + chr2.3:60702006-60702025 None:intergenic 30.0%
TACATGGTAAAAGAACATCA+AGG - chr2.3:60702632-60702651 MS.gene74612:intron 30.0%
TACTATTTCCAGATAGATGA+GGG - chr2.3:60700973-60700992 MS.gene74612:CDS 30.0%
TATCCTTAATTACAATGGTG+TGG - chr2.3:60702775-60702794 MS.gene74612:intron 30.0%
TGTGACAATCCTTTATCATT+TGG + chr2.3:60699568-60699587 None:intergenic 30.0%
TTACTATTTCCAGATAGATG+AGG - chr2.3:60700972-60700991 MS.gene74612:CDS 30.0%
TTTGATCATTTGTACAAACG+TGG - chr2.3:60702908-60702927 MS.gene74612:intron 30.0%
! AAACAGTGTTGTTAAATAGC+AGG - chr2.3:60700245-60700264 MS.gene74612:CDS 30.0%
! AGACATTTGAAACGTATACT+TGG - chr2.3:60701943-60701962 MS.gene74612:intron 30.0%
! AGTCAATTGTTTGAGATTGA+TGG - chr2.3:60701060-60701079 MS.gene74612:CDS 30.0%
! ATACTTGGTTATTTAGAGCA+TGG - chr2.3:60701958-60701977 MS.gene74612:intron 30.0%
! ATTTATGTATCAGAAGGCAT+GGG + chr2.3:60703102-60703121 None:intergenic 30.0%
! CTTTGTTTATGTTAGCCATT+AGG - chr2.3:60700103-60700122 MS.gene74612:CDS 30.0%
! GACAATTGAGGAACATATTT+TGG - chr2.3:60701760-60701779 MS.gene74612:CDS 30.0%
! TAATGATAGTATAGTGGGAA+AGG - chr2.3:60700752-60700771 MS.gene74612:CDS 30.0%
! TTTGTAACAATAGTTTCTGG+GGG + chr2.3:60702005-60702024 None:intergenic 30.0%
!! TTGGATTGATTCTAGTTCTA+GGG - chr2.3:60701779-60701798 MS.gene74612:CDS 30.0%
!! TTTGGATTGATTCTAGTTCT+AGG - chr2.3:60701778-60701797 MS.gene74612:CDS 30.0%
!!! ATATAGTTATTTTGGTGACG+TGG - chr2.3:60701806-60701825 MS.gene74612:CDS 30.0%
AAGAACCGGTAAGAAATGAT+AGG - chr2.3:60702657-60702676 MS.gene74612:intron 35.0%
AATAGCAAATCCAATTTCCC+TGG + chr2.3:60701232-60701251 None:intergenic 35.0%
AATGATTAGACTGAGAGTAG+TGG - chr2.3:60701386-60701405 MS.gene74612:CDS 35.0%
AATGCATAAACCATAGTGGT+TGG - chr2.3:60703222-60703241 MS.gene74612:intron 35.0%
AATTAGGACTCACCAGTTTA+TGG + chr2.3:60701710-60701729 None:intergenic 35.0%
AATTTCATGTTGGGAACTGT+AGG - chr2.3:60700850-60700869 MS.gene74612:CDS 35.0%
ACAAAGTAAGCAACTACTTG+AGG - chr2.3:60701002-60701021 MS.gene74612:CDS 35.0%
ACTATTTCCAGATAGATGAG+GGG - chr2.3:60700974-60700993 MS.gene74612:CDS 35.0%
AGAGAATAAAGAGACGACTA+GGG - chr2.3:60700518-60700537 MS.gene74612:CDS 35.0%
AGCTCAAATTCTAATGAAGG+AGG - chr2.3:60701603-60701622 MS.gene74612:CDS 35.0%
AGGAGACTGAAGCTAAAAAA+AGG - chr2.3:60701651-60701670 MS.gene74612:CDS 35.0%
ATACAGGATCATGATTGTGT+AGG - chr2.3:60700788-60700807 MS.gene74612:CDS 35.0%
ATCATGAGGAACATCCATAA+CGG + chr2.3:60700680-60700699 None:intergenic 35.0%
ATGCACATGACCCTTAAAAA+AGG + chr2.3:60702363-60702382 None:intergenic 35.0%
ATTCCACTAGTTGCATTTGT+TGG - chr2.3:60701867-60701886 MS.gene74612:intron 35.0%
CATAGAATGTGCAATAGTAG+AGG - chr2.3:60702037-60702056 MS.gene74612:intron 35.0%
CATGGGTGAACAAAAATGAA+TGG + chr2.3:60703085-60703104 None:intergenic 35.0%
CATGGTAAAAGAACATCAAG+GGG - chr2.3:60702634-60702653 MS.gene74612:intron 35.0%
CATTTCCAACTATGACATTG+TGG - chr2.3:60699848-60699867 MS.gene74612:CDS 35.0%
CGCAAAACTCTAAATCACAT+AGG - chr2.3:60701475-60701494 MS.gene74612:CDS 35.0%
CGGTTTGTAACAATAGTTTC+TGG + chr2.3:60702008-60702027 None:intergenic 35.0%
CGTTAGTGTGAGATAAATGA+GGG - chr2.3:60699983-60700002 MS.gene74612:CDS 35.0%
CTATTCGTGTTAAATCTTCG+TGG - chr2.3:60701247-60701266 MS.gene74612:CDS 35.0%
CTCCAAGTTCTGTGAAAAAA+TGG - chr2.3:60699942-60699961 MS.gene74612:CDS 35.0%
CTCTACTATTGCACATTCTA+TGG + chr2.3:60702039-60702058 None:intergenic 35.0%
CTCTTTCAGGATCAAATGAA+TGG + chr2.3:60701455-60701474 None:intergenic 35.0%
CTTCTCTTTACTGTTACGTT+TGG + chr2.3:60701274-60701293 None:intergenic 35.0%
GATCAATGCATAAACCATAG+TGG - chr2.3:60703218-60703237 MS.gene74612:intron 35.0%
GTGTGTTCTCCAAATGATAA+AGG - chr2.3:60699556-60699575 MS.gene74612:CDS 35.0%
GTTGGAAATGCAAATCAATC+AGG + chr2.3:60699838-60699857 None:intergenic 35.0%
TACAGGATCATGATTGTGTA+GGG - chr2.3:60700789-60700808 MS.gene74612:CDS 35.0%
TATTGCATAATGTTGGGTCA+GGG + chr2.3:60702222-60702241 None:intergenic 35.0%
TCCTTAATTACAATGGTGTG+GGG - chr2.3:60702777-60702796 MS.gene74612:intron 35.0%
TCGTTAGTGTGAGATAAATG+AGG - chr2.3:60699982-60700001 MS.gene74612:CDS 35.0%
TGCACATGACCCTTAAAAAA+GGG + chr2.3:60702362-60702381 None:intergenic 35.0%
TTATTGCATAATGTTGGGTC+AGG + chr2.3:60702223-60702242 None:intergenic 35.0%
! AACTGAAGCTCTTGATGATT+TGG - chr2.3:60702445-60702464 MS.gene74612:intron 35.0%
! AGGGCTGCATATAGTTATTT+TGG - chr2.3:60701798-60701817 MS.gene74612:CDS 35.0%
! CAATTGTTTGAGATTGATGG+TGG - chr2.3:60701063-60701082 MS.gene74612:CDS 35.0%
! CACATTCTATGGTCTTACAT+CGG + chr2.3:60702028-60702047 None:intergenic 35.0%
! TATAGTGGGAAAGGTGAAAA+CGG - chr2.3:60700761-60700780 MS.gene74612:CDS 35.0%
! TGAGAGTAGTGGAATCTAAT+AGG - chr2.3:60701397-60701416 MS.gene74612:CDS 35.0%
! TTATGCGTTTTGCGAATTTC+TGG - chr2.3:60700582-60700601 MS.gene74612:CDS 35.0%
! TTGTAACAATAGTTTCTGGG+GGG + chr2.3:60702004-60702023 None:intergenic 35.0%
!! TGAATGATGATGGACAATTG+AGG - chr2.3:60701748-60701767 MS.gene74612:CDS 35.0%
!! TTTGTTGGTGTTAATCACCA+TGG - chr2.3:60701882-60701901 MS.gene74612:intron 35.0%
!!! CACCATTTTTTCACAGAACT+TGG + chr2.3:60699947-60699966 None:intergenic 35.0%
!!! TTTTTTTAGCTTCAGTCTCC+TGG + chr2.3:60701652-60701671 None:intergenic 35.0%
AAATAAAAGGGAGTGGTGCA+TGG - chr2.3:60700044-60700063 MS.gene74612:CDS 40.0%
AACCATGACTGCAAGACAAT+TGG + chr2.3:60700019-60700038 None:intergenic 40.0%
AACTATATCTTCCACACGAG+TGG + chr2.3:60700712-60700731 None:intergenic 40.0%
AATAGCTATGCCAGGGAAAT+TGG - chr2.3:60701219-60701238 MS.gene74612:CDS 40.0%
AATGTGTCCTTGGAGTAAAC+TGG + chr2.3:60702296-60702315 None:intergenic 40.0%
AATTTGTGAGACGCTGAAAC+AGG + chr2.3:60702588-60702607 None:intergenic 40.0%
ACACCAACAAATGCAACTAG+TGG + chr2.3:60701873-60701892 None:intergenic 40.0%
ACCAAACACATATTTCGAGC+AGG + chr2.3:60703281-60703300 None:intergenic 40.0%
ACTTGCCACAATGTCATAGT+TGG + chr2.3:60699856-60699875 None:intergenic 40.0%
AGAATTTGAGCTCCCATAAC+CGG + chr2.3:60701595-60701614 None:intergenic 40.0%
AGTGGAATCTAATAGGTGGA+GGG - chr2.3:60701404-60701423 MS.gene74612:CDS 40.0%
ATATTTCGAGCAGGGTTTGA+TGG + chr2.3:60703272-60703291 None:intergenic 40.0%
ATCAAAACACTGAACTGGGT+TGG + chr2.3:60702894-60702913 None:intergenic 40.0%
ATCACAACCAAGCACAAAGA+TGG + chr2.3:60700876-60700895 None:intergenic 40.0%
ATGGATGTTCCTCATGATCT+TGG - chr2.3:60700682-60700701 MS.gene74612:CDS 40.0%
ATTTGATCCGCCTTGAATCA+TGG + chr2.3:60700460-60700479 None:intergenic 40.0%
CAATAAGTTCCAAGATGAGG+TGG - chr2.3:60702550-60702569 MS.gene74612:intron 40.0%
CAGAGAATAAAGAGACGACT+AGG - chr2.3:60700517-60700536 MS.gene74612:CDS 40.0%
CCAAACACATATTTCGAGCA+GGG + chr2.3:60703280-60703299 None:intergenic 40.0%
CCTTAATTACAATGGTGTGG+GGG - chr2.3:60702778-60702797 MS.gene74612:intron 40.0%
CGACTCTAGATGTTGAAGTA+CGG - chr2.3:60701532-60701551 MS.gene74612:CDS 40.0%
CGAGTCTATAGTGACATACA+TGG - chr2.3:60702616-60702635 MS.gene74612:intron 40.0%
CGTAGGAAAGAAACAAGAAC+AGG - chr2.3:60701354-60701373 MS.gene74612:CDS 40.0%
CTAGCAAAACAGATTGACCA+TGG + chr2.3:60701902-60701921 None:intergenic 40.0%
CTCTAAATCACATAGGAGGA+TGG - chr2.3:60701482-60701501 MS.gene74612:CDS 40.0%
GCTCGAAATATGTGTTTGGT+GGG - chr2.3:60703281-60703300 MS.gene74612:CDS 40.0%
GGGAGCTCAAATTCTAATGA+AGG - chr2.3:60701600-60701619 MS.gene74612:CDS 40.0%
GGTATGCAAGTAGTTGAAGA+AGG - chr2.3:60702929-60702948 MS.gene74612:intron 40.0%
GTCTTGTGTTGTAGTTGTGA+AGG - chr2.3:60701303-60701322 MS.gene74612:CDS 40.0%
GTTAGCCATTAGGATGTCAT+AGG - chr2.3:60700113-60700132 MS.gene74612:CDS 40.0%
TAAGCAACTACTTGAGGTTG+AGG - chr2.3:60701008-60701027 MS.gene74612:CDS 40.0%
TAGAGAGTTGCAACTCATAG+TGG + chr2.3:60702508-60702527 None:intergenic 40.0%
TAGTGGAATCTAATAGGTGG+AGG - chr2.3:60701403-60701422 MS.gene74612:CDS 40.0%
TCCAGCAAAAAATGTGTCCT+TGG + chr2.3:60702306-60702325 None:intergenic 40.0%
TCGTGTGGAAGATATAGTTG+TGG - chr2.3:60700713-60700732 MS.gene74612:CDS 40.0%
TGCTATGCTGAAACTGTATG+CGG - chr2.3:60700292-60700311 MS.gene74612:CDS 40.0%
TGCTCGAAATATGTGTTTGG+TGG - chr2.3:60703280-60703299 MS.gene74612:CDS 40.0%
TGGCGAACATGAAAATGGAA+GGG - chr2.3:60701083-60701102 MS.gene74612:CDS 40.0%
TTACCGTGATGTCGAGATTA+GGG + chr2.3:60699594-60699613 None:intergenic 40.0%
TTACTTCTGCCACCATAAAC+TGG - chr2.3:60701695-60701714 MS.gene74612:CDS 40.0%
TTTACCGTGATGTCGAGATT+AGG + chr2.3:60699595-60699614 None:intergenic 40.0%
! AATAGTAGAGGTTTTCCCGA+GGG - chr2.3:60702049-60702068 MS.gene74612:intron 40.0%
! ATAGTGGGAAAGGTGAAAAC+GGG - chr2.3:60700762-60700781 MS.gene74612:CDS 40.0%
! CAATCTGTTTTGCTAGGATG+TGG - chr2.3:60701906-60701925 MS.gene74612:intron 40.0%
! CATGGTCAATCTGTTTTGCT+AGG - chr2.3:60701900-60701919 MS.gene74612:intron 40.0%
! CGTACTTCAACATCTAGAGT+CGG + chr2.3:60701534-60701553 None:intergenic 40.0%
! GAGTAGTGGAATCTAATAGG+TGG - chr2.3:60701400-60701419 MS.gene74612:CDS 40.0%
! GCAACAACGTTAAGTTTTCC+AGG - chr2.3:60701631-60701650 MS.gene74612:CDS 40.0%
! TGTGCTTTTCAGTCTGATGT+TGG - chr2.3:60699655-60699674 MS.gene74612:intron 40.0%
! TTAGAGTTTTGCGCTCTTTC+AGG + chr2.3:60701468-60701487 None:intergenic 40.0%
! TTTTCAGTCTGATGTTGGAC+TGG - chr2.3:60699660-60699679 MS.gene74612:intron 40.0%
!! GTGATGGATCTGAATGATGA+TGG - chr2.3:60701738-60701757 MS.gene74612:CDS 40.0%
!!! TCCAAGGACACATTTTTTGC+TGG - chr2.3:60702302-60702321 MS.gene74612:intron 40.0%
!!! TGCGAATTTCTGGTGTTTTG+CGG - chr2.3:60700592-60700611 MS.gene74612:CDS 40.0%
AAATGGAAGGGAGGAAACCA+CGG - chr2.3:60701095-60701114 MS.gene74612:CDS 45.0%
AAATTCTATGGTGAGCCCTC+GGG + chr2.3:60702067-60702086 None:intergenic 45.0%
AAGCCCTAATCTCGACATCA+CGG - chr2.3:60699588-60699607 MS.gene74612:intron 45.0%
ACATCATCACCACCTCATCT+TGG + chr2.3:60702562-60702581 None:intergenic 45.0%
ACATTGTCAGAGCTGAGATG+TGG + chr2.3:60702863-60702882 None:intergenic 45.0%
ACTACTTGAGGTTGAGGACA+AGG - chr2.3:60701014-60701033 MS.gene74612:CDS 45.0%
ACTGTAGATGCTTCCGGTTA+TGG - chr2.3:60701579-60701598 MS.gene74612:CDS 45.0%
ACTTCAAAGGCTACCTATGC+AGG - chr2.3:60702741-60702760 MS.gene74612:intron 45.0%
AGAACATCAAGGGGAAGAAC+CGG - chr2.3:60702643-60702662 MS.gene74612:intron 45.0%
AGGCACCTATGACATCCTAA+TGG + chr2.3:60700121-60700140 None:intergenic 45.0%
AGGTGAAAACGGGTAGATAC+AGG - chr2.3:60700772-60700791 MS.gene74612:CDS 45.0%
ATATCCCCTATTGCCGATCT+GGG + chr2.3:60700332-60700351 None:intergenic 45.0%
ATGCCAACTGTAGATGCTTC+CGG - chr2.3:60701573-60701592 MS.gene74612:CDS 45.0%
CAAATTCTATGGTGAGCCCT+CGG + chr2.3:60702068-60702087 None:intergenic 45.0%
CACCAATTGTCTTGCAGTCA+TGG - chr2.3:60700014-60700033 MS.gene74612:CDS 45.0%
CCCCCACACCATTGTAATTA+AGG + chr2.3:60702781-60702800 None:intergenic 45.0%
CCCTGCTCGAAATATGTGTT+TGG - chr2.3:60703277-60703296 MS.gene74612:CDS 45.0%
CGAACATGAAAATGGAAGGG+AGG - chr2.3:60701086-60701105 MS.gene74612:CDS 45.0%
CGTAACAGTAAAGAGAAGCG+TGG - chr2.3:60701276-60701295 MS.gene74612:CDS 45.0%
CTACTTGAGGTTGAGGACAA+GGG - chr2.3:60701015-60701034 MS.gene74612:CDS 45.0%
CTGCATTGTCGGTCATGATT+CGG + chr2.3:60699537-60699556 None:intergenic 45.0%
CTGTAGATGCTTCCGGTTAT+GGG - chr2.3:60701580-60701599 MS.gene74612:CDS 45.0%
GGTTCGTTGTGTAACAGACA+AGG - chr2.3:60703012-60703031 MS.gene74612:intron 45.0%
GTGGCGAACATGAAAATGGA+AGG - chr2.3:60701082-60701101 MS.gene74612:CDS 45.0%
TAGGACTCACCAGTTTATGG+TGG + chr2.3:60701707-60701726 None:intergenic 45.0%
TATACAAGGCATGCCTGCAT+AGG + chr2.3:60702757-60702776 None:intergenic 45.0%
TATATCCCCTATTGCCGATC+TGG + chr2.3:60700333-60700352 None:intergenic 45.0%
TGCTATTCCCAATCCCAGAT+CGG - chr2.3:60700316-60700335 MS.gene74612:CDS 45.0%
TGGAGAACACACTGCATTGT+CGG + chr2.3:60699548-60699567 None:intergenic 45.0%
! CAATAGTAGAGGTTTTCCCG+AGG - chr2.3:60702048-60702067 MS.gene74612:intron 45.0%
! GATGGTGGCGAACATGAAAA+TGG - chr2.3:60701078-60701097 MS.gene74612:CDS 45.0%
! GGAAGATGAGGCATTTCAGA+TGG - chr2.3:60702136-60702155 MS.gene74612:intron 45.0%
!! AGTCGGCTCAACCTTTCTTT+TGG + chr2.3:60701517-60701536 None:intergenic 45.0%
!! TAACCGGAAGCATCTACAGT+TGG + chr2.3:60701579-60701598 None:intergenic 45.0%
!!! TGGCATCTTGTTTTCAGCTG+TGG + chr2.3:60702488-60702507 None:intergenic 45.0%
AATGGAAGGGAGGAAACCAC+GGG - chr2.3:60701096-60701115 MS.gene74612:CDS 50.0%
ACATAGGAGGATGGACAGTG+GGG - chr2.3:60701491-60701510 MS.gene74612:CDS 50.0%
ACCTGCATCACCAACCACTA+TGG + chr2.3:60703235-60703254 None:intergenic 50.0%
ACTATGACTGTCAACTCCCG+TGG + chr2.3:60701115-60701134 None:intergenic 50.0%
AGATCGATGCCAGTCCGTTA+TGG - chr2.3:60700663-60700682 MS.gene74612:CDS 50.0%
AGTGGACAGCCAAGATCATG+AGG + chr2.3:60700694-60700713 None:intergenic 50.0%
CAAGTCTTGCAGATCATCCG+TGG - chr2.3:60702095-60702114 MS.gene74612:intron 50.0%
CAATCCCAGATCGGCAATAG+GGG - chr2.3:60700325-60700344 MS.gene74612:CDS 50.0%
CACATAGGAGGATGGACAGT+GGG - chr2.3:60701490-60701509 MS.gene74612:CDS 50.0%
CCAATCCCAGATCGGCAATA+GGG - chr2.3:60700324-60700343 MS.gene74612:CDS 50.0%
CTGTAGGCCATCTTTGTGCT+TGG - chr2.3:60700866-60700885 MS.gene74612:CDS 50.0%
GAAACTGCACCAGACAACCA+CGG + chr2.3:60702115-60702134 None:intergenic 50.0%
GATGCAGGTTGCAGCTGTTA+AGG - chr2.3:60703246-60703265 MS.gene74612:CDS 50.0%
GGAATCTAATAGGTGGAGGG+TGG - chr2.3:60701407-60701426 MS.gene74612:CDS 50.0%
GGTGCAGTTTCAGGAAGATG+AGG - chr2.3:60702124-60702143 MS.gene74612:intron 50.0%
GTAGAGACCCCTCATCTATC+TGG + chr2.3:60700984-60701003 None:intergenic 50.0%
TCAAACTCCACTCCCTCAAC+AGG + chr2.3:60701171-60701190 None:intergenic 50.0%
TCACATAGGAGGATGGACAG+TGG - chr2.3:60701489-60701508 MS.gene74612:CDS 50.0%
TGGTTGTCTGGTGCAGTTTC+AGG - chr2.3:60702115-60702134 MS.gene74612:intron 50.0%
TTGGGCTCCAGTTTACTCCA+AGG - chr2.3:60702286-60702305 MS.gene74612:intron 50.0%
! CCTATTGCCGATCTGGGATT+GGG + chr2.3:60700326-60700345 None:intergenic 50.0%
!! ACCATAGTGGTTGGTGATGC+AGG - chr2.3:60703231-60703250 MS.gene74612:intron 50.0%
!! GAGGAACATCCATAACGGAC+TGG + chr2.3:60700675-60700694 None:intergenic 50.0%
AAAGCTCCGAAGCTTGCACG+AGG - chr2.3:60702253-60702272 MS.gene74612:intron 55.0%
ACTCCACTCCCTCAACAGGT+TGG + chr2.3:60701167-60701186 None:intergenic 55.0%
AGACTACGCCCAACCTGTTG+AGG - chr2.3:60701155-60701174 MS.gene74612:CDS 55.0%
ATCTTGGCTGTCCACTCGTG+TGG - chr2.3:60700698-60700717 MS.gene74612:CDS 55.0%
CCCAATCCCAGATCGGCAAT+AGG - chr2.3:60700323-60700342 MS.gene74612:CDS 55.0%
CTCCACTCCCTCAACAGGTT+GGG + chr2.3:60701166-60701185 None:intergenic 55.0%
CTCTGTCCTCGTGCAAGCTT+CGG + chr2.3:60702262-60702281 None:intergenic 55.0%
GACTACGCCCAACCTGTTGA+GGG - chr2.3:60701156-60701175 MS.gene74612:CDS 55.0%
GCACGAGGACAGAGAGCTTT+GGG - chr2.3:60702268-60702287 MS.gene74612:intron 55.0%
GCAGATCATCCGTGGTTGTC+TGG - chr2.3:60702103-60702122 MS.gene74612:intron 55.0%
GGACAGTGGGGCCAAAAGAA+AGG - chr2.3:60701503-60701522 MS.gene74612:CDS 55.0%
TGCACGAGGACAGAGAGCTT+TGG - chr2.3:60702267-60702286 MS.gene74612:intron 55.0%
! CCCTATTGCCGATCTGGGAT+TGG + chr2.3:60700327-60700346 None:intergenic 55.0%
CGCCCAACCTGTTGAGGGAG+TGG - chr2.3:60701161-60701180 MS.gene74612:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 60699514 60703336 60699514 ID=MS.gene74612
chr2.3 mRNA 60699514 60703336 60699514 ID=MS.gene74612.t1;Parent=MS.gene74612
chr2.3 exon 60703241 60703336 60703241 ID=MS.gene74612.t1.exon1;Parent=MS.gene74612.t1
chr2.3 CDS 60703241 60703336 60703241 ID=cds.MS.gene74612.t1;Parent=MS.gene74612.t1
chr2.3 exon 60699838 60701865 60699838 ID=MS.gene74612.t1.exon2;Parent=MS.gene74612.t1
chr2.3 CDS 60699838 60701865 60699838 ID=cds.MS.gene74612.t1;Parent=MS.gene74612.t1
chr2.3 exon 60699514 60699597 60699514 ID=MS.gene74612.t1.exon3;Parent=MS.gene74612.t1
chr2.3 CDS 60699514 60699597 60699514 ID=cds.MS.gene74612.t1;Parent=MS.gene74612.t1
Gene Sequence

>MS.gene74612

AACTCATGTGAACTCTCTGGCCGAATCATGACCGACAATGCAGTGTGTTCTCCAAATGATAAAGGATTGTCACAAAGCCCTAATCTCGACATCACGGTAAATCTTCATCTTAACCATGGAACTTAATGACTTGTTTTATGCTGTGCTTTTCAGTCTGATGTTGGACTGGATCTTATTTGTCTATCTGCCTCTAACCCAAACTATTTATTTGCAAACAACTCACGCTTTTGTTGAGAAAAGAAAAGAGGAATCAAATTAACGCCAAACTCTGAATTTTTAATCTTTTAAGACTTTAGTGTACTGTATAGTTTTGCTAAATTTCCTGATTGATTTGCATTTCCAACTATGACATTGTGGCAAGTCTTGAAGAACACGATACTCGATTGAGATACTTTTGTAATTTTCTGTTTTTTAAGTTCAAAGTGGTTCTCCAAGTTCTGTGAAAAAATGGTGCAATGTACAATTTTTTCGTTAGTGTGAGATAAATGAGGGAAGAAGTGCACCAATTGTCTTGCAGTCATGGTTACTTAAAATAAAAGGGAGTGGTGCATGGATTTTAATTATCCTGTTTATCACTTCAAATTTTTGTCTTTGTTTATGTTAGCCATTAGGATGTCATAGGTGCCTAATGACATTGATTATTTCACATAATTACTTTGGAATATTTTTTATTGATTAACAGGCATAGAATAATTGTTTTTGAAGAAGGGCATAGAATAATTGTTGATTATAAACAGTGTTGTTAAATAGCAGGATGTGAACAAAAAACTATGAAAACTGCTATGCTGAAACTGTATGCGGCTGCTATTCCCAATCCCAGATCGGCAATAGGGGATATAGCATTAATTCAAAGAGAAAAAAAATTGTGGGTTTTTTTAATGATAACACTGATAGGGGAGCATGTGAGCTGCACATTACCCCTTTAAAATTGTTAACAATATAACCATGATTCAAGGCGGATCAAATCATGCTCTTGATACCATATTAACAATATAAGTTTCAACAGAGAATAAAGAGACGACTAGGGTTCCAATAATATCAATGTCATAACATAATATAATAGCGGATTTATGCGTTTTGCGAATTTCTGGTGTTTTGCGGATTTCTGTGATCCTTTATTTATAACACTGATTATAAATCATTTATCTGAGATCGATGCCAGTCCGTTATGGATGTTCCTCATGATCTTGGCTGTCCACTCGTGTGGAAGATATAGTTGTGGTATTGCTTCAAAGATATAATGATAGTATAGTGGGAAAGGTGAAAACGGGTAGATACAGGATCATGATTGTGTAGGGTGAAAATAAAAGAAACTTCACACCTGTAAGTAATATAAAATTTCATGTTGGGAACTGTAGGCCATCTTTGTGCTTGGTTGTGATATTTCAAGTTCTGTTGTCAAATTCAATGCATACTCAAATTTCTTAGACTGTGTTTAGAATTTGTTTTTTTATTTCATTACTATTTCCAGATAGATGAGGGGTCTCTACAAAGTAAGCAACTACTTGAGGTTGAGGACAAGGGCAACGAGCTTGAGAATGACTGTAGTCAATTGTTTGAGATTGATGGTGGCGAACATGAAAATGGAAGGGAGGAAACCACGGGAGTTGACAGTCATAGTGAAGAATCTCAAAGAACAAAAGACTACGCCCAACCTGTTGAGGGAGTGGAGTTTGAAACTTATGATGATGCCTACAACTATTATAATAGCTATGCCAGGGAAATTGGATTTGCTATTCGTGTTAAATCTTCGTGGACCAAACGTAACAGTAAAGAGAAGCGTGGTGCTGTCTTGTGTTGTAGTTGTGAAGGTTTCAAAACAAATAAAGAAGCTAATAGTCGTAGGAAAGAAACAAGAACAGGCTGTCTAGCAATGATTAGACTGAGAGTAGTGGAATCTAATAGGTGGAGGGTGGATGAAGTCAAGCTTGAGCATAACCATTCATTTGATCCTGAAAGAGCGCAAAACTCTAAATCACATAGGAGGATGGACAGTGGGGCCAAAAGAAAGGTTGAGCCGACTCTAGATGTTGAAGTACGGACAATCAAGTTATATCGAATGCCAACTGTAGATGCTTCCGGTTATGGGAGCTCAAATTCTAATGAAGGAGGAACTAGCAACAACGTTAAGTTTTCCAGGAGACTGAAGCTAAAAAAAGGTGATGCAGAACTTATTTCAAATTACTTCTGCCACCATAAACTGGTGAGTCCTAATTTTTTCTATGTGATGGATCTGAATGATGATGGACAATTGAGGAACATATTTTGGATTGATTCTAGTTCTAGGGCTGCATATAGTTATTTTGGTGACGTGGTTGCATTTGACTCAACATATTTGTCAAACAATTATGAGATTCCACTAGTTGCATTTGTTGGTGTTAATCACCATGGTCAATCTGTTTTGCTAGGATGTGGTCTGCTTGCAGATGAGACATTTGAAACGTATACTTGGTTATTTAGAGCATGGCTTTCTTGTATGTCTAGTCGCCCCCCAGAAACTATTGTTACAAACCGATGTAAGACCATAGAATGTGCAATAGTAGAGGTTTTCCCGAGGGCTCACCATAGAATTTGTCTATTGCAAGTCTTGCAGATCATCCGTGGTTGTCTGGTGCAGTTTCAGGAAGATGAGGCATTTCAGATGGCACTGACAAGAGTGATATATGACCCTAAAACATTAGATGAATTTGAAAGAGATTGGGATTCCCTGACCCAACATTATGCAATAAGAAATCATGCAAAGCTCCGAAGCTTGCACGAGGACAGAGAGCTTTGGGCTCCAGTTTACTCCAAGGACACATTTTTTGCTGGAATTTCTAATTACGAAAAGGGTGAATCTATGATTCCCTTTTTTAAGGGTCATGTGCATCAACAAACTTCTTTGAAAGAATTTTTTGAGATTTATGAATTAGTTCAACAAAAAAAGCAAAAAACTGAAGCTCTTGATGATTTGGAGTCACAAAATTCAAGTCCACAGCTGAAAACAAGATGCCACTATGAGTTGCAACTCTCTAAATTGTACACGAATGCAATATTCAATAAGTTCCAAGATGAGGTGGTGATGATGTCTTCCTGTTTCAGCGTCTCACAAATTCAAACAAACGAGTCTATAGTGACATACATGGTAAAAGAACATCAAGGGGAAGAACCGGTAAGAAATGATAGGCATTTTGAAGTCATGTATGATAAAGCATTAACCGAAGTACGTTGCATTTGTAATTGCTTTAACTTCAAAGGCTACCTATGCAGGCATGCCTTGTATATCCTTAATTACAATGGTGTGGGGGAAATTCCATGTCATTATATTTTGTCAAGATGGAGGAAAGATTTTAAGCGATTGTATGTACCACATCTCAGCTCTGACAATGTTGATATTACCAACCCAGTTCAGTGTTTTGATCATTTGTACAAACGTGGTATGCAAGTAGTTGAAGAAGGCACGATATCCCAAAATCATTATTTGGTTTCTTGGCAGACATTTATAGAGTCTTTGAATAAGGTTCGTTGTGTAACAGACAAGGTTGAATAAATAAATCAGAAATAATGCTAATATGTTGTGCATAAGGTACCATTCATTTTTGTTCACCCATGCCTTCTGATACATAAATTGTTCTCTTATAGTTATAGTTTATGTTAAAAGTTTGCAGAATTATGTGAAAGCATAGAATGTGAATGTCTTTTTGGTTCGTTTACTAAATCAATTTGATCAATGCATAAACCATAGTGGTTGGTGATGCAGGTTGCAGCTGTTAAGGCCATCAAACCCTGCTCGAAATATGTGTTTGGTGGGCCTACATACATGAATTATGATAAAGAGAAATAG
Protein sequence

>MS.gene74612.t1

NSCELSGRIMTDNAVCSPNDKGLSQSPNLDITIDEGSLQSKQLLEVEDKGNELENDCSQLFEIDGGEHENGREETTGVDSHSEESQRTKDYAQPVEGVEFETYDDAYNYYNSYAREIGFAIRVKSSWTKRNSKEKRGAVLCCSCEGFKTNKEANSRRKETRTGCLAMIRLRVVESNRWRVDEVKLEHNHSFDPERAQNSKSHRRMDSGAKRKVEPTLDVEVRTIKLYRMPTVDASGYGSSNSNEGGTSNNVKFSRRLKLKKGDAELISNYFCHHKLVSPNFFYVMDLNDDGQLRNIFWIDSSSRAAYSYFGDVVAFDSTYLSNNYEIPLVAFVGVNHHGQSVLLGCGLLADETFETYTWLFRAWLSCMSSRPPETIVTNRCKTIECAIVEVFPRAHHRICLLQVLQIIRGCLVQFQEDEAFQMALTRVIYDPKTLDEFERDWDSLTQHYAIRNHAKLRSLHEDRELWAPVYSKDTFFAGISNYEKGESMIPFFKGHVHQQTSLKEFFEIYELVQQKKQKTEALDDLESQNSSPQLKTRCHYELQLSKLYTNAIFNKFQDEVVMMSSCFSVSQIQTNESIVTYMVKEHQGEEPVRNDRHFEVMYDKALTEVRCICNCFNFKGYLCRHALYILNYNGVGEIPCHYILSRWRKDFKRLYVPHLSSDNVDITNPVQCFDHLYKRGMQVVEEGTISQNHYLVSWQTFIESLNKVAAVKAIKPCSKYVFGGPTYMNYDKEK