AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74841.t1 MTR_1g078260 73.289 453 48 6 8 445 90 484 0.0 605
MS.gene74841.t1 MTR_6g086555 50.000 356 163 6 16 369 59 401 1.72e-105 319
MS.gene74841.t1 MTR_7g071120 32.547 212 124 7 80 277 225 431 4.50e-22 99.0
MS.gene74841.t1 MTR_7g011820 51.852 81 38 1 142 222 387 466 5.81e-22 98.6
MS.gene74841.t1 MTR_7g011820 43.023 86 43 3 165 250 228 307 1.01e-11 67.4
MS.gene74841.t1 MTR_7g080020 49.474 95 44 3 129 222 458 549 1.44e-21 98.2
MS.gene74841.t1 MTR_3g009470 40.876 137 73 4 104 240 439 567 3.78e-21 96.7
MS.gene74841.t1 MTR_3g104750 52.055 73 34 1 150 222 89 160 9.69e-21 89.4
MS.gene74841.t1 MTR_7g080000 54.930 71 31 1 152 222 419 488 1.68e-20 94.7
MS.gene74841.t1 MTR_5g042920 47.423 97 50 1 147 243 302 397 2.35e-20 93.2
MS.gene74841.t1 MTR_4g013260 45.714 105 55 2 118 222 290 392 2.35e-20 93.6
MS.gene74841.t1 MTR_4g082580 46.875 96 49 2 128 222 240 334 2.74e-20 92.8
MS.gene74841.t1 MTR_5g094430 37.956 137 81 2 86 222 288 420 3.65e-20 93.6
MS.gene74841.t1 MTR_3g095080 52.000 75 35 1 150 224 153 226 6.37e-20 89.0
MS.gene74841.t1 MTR_1g015140 50.667 75 36 1 148 222 85 158 6.97e-20 86.7
MS.gene74841.t1 MTR_8g092010 51.389 72 34 1 151 222 94 164 7.98e-20 87.0
MS.gene74841.t1 MTR_5g094430 37.956 137 81 2 86 222 176 308 9.35e-20 91.7
MS.gene74841.t1 MTR_5g094430 37.956 137 81 2 86 222 154 286 1.03e-19 91.3
MS.gene74841.t1 MTR_3g095080 52.000 75 35 1 150 224 129 202 1.18e-19 87.8
MS.gene74841.t1 MTR_8g092140 53.521 71 32 1 157 227 152 221 1.89e-19 87.4
MS.gene74841.t1 MTR_5g074400 52.500 80 37 1 143 222 398 476 1.93e-19 91.3
MS.gene74841.t1 MTR_3g056100 53.425 73 33 1 150 222 387 458 2.95e-19 90.9
MS.gene74841.t1 MTR_3g056100 53.425 73 33 1 150 222 400 471 3.43e-19 90.5
MS.gene74841.t1 MTR_3g031220 54.795 73 32 1 150 222 350 421 5.74e-19 89.7
MS.gene74841.t1 MTR_3g031220 43.333 90 42 4 165 253 198 279 5.03e-11 65.1
MS.gene74841.t1 MTR_3g056100 53.425 73 33 1 150 222 243 314 6.21e-19 89.0
MS.gene74841.t1 MTR_7g109800 41.905 105 48 3 150 253 130 222 7.59e-19 87.0
MS.gene74841.t1 MTR_3g056100 53.425 73 33 1 150 222 256 327 7.94e-19 89.0
MS.gene74841.t1 MTR_8g007270 48.718 78 39 1 150 227 139 215 8.92e-18 82.4
MS.gene74841.t1 MTR_3g095040 48.000 75 38 1 148 222 101 174 1.19e-17 81.6
MS.gene74841.t1 MTR_7g079010 50.704 71 34 1 152 222 128 197 1.39e-17 81.6
MS.gene74841.t1 MTR_7g100510 51.351 74 35 1 149 222 345 417 2.07e-17 84.3
MS.gene74841.t1 MTR_4g094588 43.689 103 50 4 152 254 165 259 2.50e-17 83.2
MS.gene74841.t1 MTR_4g122530 48.684 76 37 2 152 226 195 269 4.19e-17 83.6
MS.gene74841.t1 MTR_1g086790 49.315 73 36 1 150 222 143 214 7.01e-17 81.6
MS.gene74841.t1 MTR_5g097490 45.333 75 40 1 150 224 237 310 7.67e-17 82.4
MS.gene74841.t1 MTR_7g028415 52.857 70 28 2 155 222 83 149 1.88e-16 77.4
MS.gene74841.t1 MTR_7g028710 52.857 70 28 2 155 222 83 149 1.88e-16 77.4
MS.gene74841.t1 MTR_2g033820 49.315 73 36 1 150 222 188 259 2.59e-16 80.9
MS.gene74841.t1 MTR_7g062220 46.154 78 40 2 146 222 104 180 3.89e-15 76.6
MS.gene74841.t1 MTR_5g043880 45.946 74 39 1 152 225 111 183 5.28e-15 75.5
MS.gene74841.t1 MTR_5g091390 46.479 71 37 1 152 222 144 213 8.66e-15 75.5
MS.gene74841.t1 MTR_8g096730 44.595 74 40 1 149 222 54 126 9.26e-15 71.6
MS.gene74841.t1 MTR_1g077780 50.685 73 35 1 150 222 47 118 2.01e-14 70.9
MS.gene74841.t1 MTR_7g110720 35.652 115 68 3 113 222 133 246 5.30e-14 73.9
MS.gene74841.t1 MTR_7g110720 38.235 102 57 3 126 222 173 273 6.44e-14 73.6
MS.gene74841.t1 MTR_4g130900 38.462 104 62 2 128 230 321 423 7.12e-14 73.9
MS.gene74841.t1 MTR_2g088000 40.909 88 42 3 145 223 321 407 1.01e-13 73.6
MS.gene74841.t1 MTR_4g132430 43.056 72 39 2 152 222 114 184 1.97e-13 71.6
MS.gene74841.t1 MTR_2g045360 27.604 192 127 6 41 222 79 268 4.17e-13 71.2
MS.gene74841.t1 MTR_8g028565 39.216 102 45 4 122 222 127 212 4.84e-13 70.5
MS.gene74841.t1 MTR_3g108960 40.594 101 47 4 123 222 283 371 5.06e-13 70.9
MS.gene74841.t1 MTR_4g132430 43.056 72 39 2 152 222 248 318 1.12e-12 70.1
MS.gene74841.t1 MTR_2g023930 43.056 72 39 2 152 222 292 362 1.16e-12 70.1
MS.gene74841.t1 MTR_3g053330 42.466 73 40 2 152 223 238 309 1.19e-12 70.1
MS.gene74841.t1 MTR_1g009613 38.532 109 47 4 119 222 226 319 1.76e-12 68.6
MS.gene74841.t1 MTR_4g049790 31.061 132 70 4 111 222 239 369 1.84e-12 69.7
MS.gene74841.t1 MTR_8g098945 41.837 98 48 4 164 260 128 217 5.06e-12 66.6
MS.gene74841.t1 MTR_2g016770 54.237 59 25 2 166 223 36 93 5.36e-12 65.5
MS.gene74841.t1 MTR_2g016770 55.172 58 24 2 166 222 28 84 6.95e-12 65.1
MS.gene74841.t1 MTR_3g090860 50.685 73 29 3 155 221 120 191 1.12e-11 66.2
MS.gene74841.t1 MTR_2g105060 40.541 74 40 3 152 222 154 226 1.42e-11 65.9
MS.gene74841.t1 MTR_2g105060 40.541 74 40 3 152 222 79 151 1.45e-11 64.7
MS.gene74841.t1 MTR_4g107970 41.096 73 41 2 152 223 165 236 1.75e-11 66.6
MS.gene74841.t1 MTR_7g109600 51.724 58 26 2 166 222 54 110 2.14e-11 64.7
MS.gene74841.t1 MTR_1g013760 40.000 75 41 3 152 223 125 198 2.56e-11 64.7
MS.gene74841.t1 MTR_3g031220 43.333 90 42 4 165 253 198 279 2.74e-11 65.5
MS.gene74841.t1 MTR_4g127040 46.970 66 33 2 162 226 242 306 3.63e-11 64.3
MS.gene74841.t1 MTR_0117s0040 49.254 67 32 2 162 227 71 136 3.68e-11 63.5
MS.gene74841.t1 MTR_4g107970 41.096 73 41 2 152 223 242 313 3.76e-11 65.5
MS.gene74841.t1 MTR_5g016610 37.037 108 48 4 121 223 165 257 4.29e-11 63.9
MS.gene74841.t1 MTR_1g070455 49.231 65 31 2 162 225 290 353 4.84e-11 64.3
MS.gene74841.t1 MTR_7g009730 43.056 72 39 2 152 222 206 276 5.41e-11 65.1
MS.gene74841.t1 MTR_4g049760 41.558 77 43 2 147 222 272 347 5.45e-11 65.1
MS.gene74841.t1 MTR_2g032030 45.455 66 34 2 158 222 260 324 6.12e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74841.t1 AT5G64810 38.835 103 62 1 120 222 65 166 1.15e-21 92.8
MS.gene74841.t1 AT1G64000 55.556 72 31 1 151 222 100 170 2.09e-21 92.0
MS.gene74841.t1 AT2G38470 41.538 130 70 3 95 222 293 418 4.47e-21 96.3
MS.gene74841.t1 AT5G41570 54.930 71 31 1 152 222 85 154 1.25e-20 89.4
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 326 397 2.61e-20 94.0
MS.gene74841.t1 AT2G03340 37.500 144 80 4 82 222 335 471 2.77e-20 93.6
MS.gene74841.t1 AT5G07100 52.381 84 32 2 150 226 219 301 3.50e-20 91.3
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 366 437 3.57e-20 93.6
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 294 365 3.69e-20 93.2
MS.gene74841.t1 AT3G01080 53.947 76 34 1 147 222 326 400 4.43e-20 92.8
MS.gene74841.t1 AT1G13960 53.947 76 34 1 147 222 391 465 4.59e-20 93.2
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 190 261 5.13e-20 91.7
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 190 261 5.13e-20 91.7
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 190 261 5.13e-20 91.7
MS.gene74841.t1 AT4G26640 54.795 73 32 1 150 222 190 261 5.13e-20 91.7
MS.gene74841.t1 AT5G07100 52.381 84 32 2 150 226 126 208 5.85e-20 88.6
MS.gene74841.t1 AT5G07100 52.381 84 32 2 150 226 126 208 5.85e-20 88.6
MS.gene74841.t1 AT5G07100 52.381 84 32 2 150 226 126 208 5.85e-20 88.6
MS.gene74841.t1 AT5G07100 52.381 84 32 2 150 226 126 208 5.85e-20 88.6
MS.gene74841.t1 AT3G01080 53.947 76 34 1 147 222 288 362 7.12e-20 91.7
MS.gene74841.t1 AT4G26440 39.394 132 75 3 91 222 302 428 1.34e-19 91.7
MS.gene74841.t1 AT1G13960 53.947 76 34 1 147 222 238 312 1.58e-19 90.1
MS.gene74841.t1 AT5G56270 54.795 73 32 1 150 222 472 543 3.37e-19 90.9
MS.gene74841.t1 AT1G55600 52.632 76 35 1 147 222 289 363 9.36e-19 89.0
MS.gene74841.t1 AT2G04880 38.017 121 74 1 102 222 220 339 1.75e-18 87.8
MS.gene74841.t1 AT2G04880 38.017 121 74 1 102 222 244 363 2.30e-18 87.8
MS.gene74841.t1 AT2G46130 52.113 71 33 1 152 222 17 86 4.26e-18 80.1
MS.gene74841.t1 AT2G44745 39.623 106 52 2 139 233 108 212 4.61e-18 83.2
MS.gene74841.t1 AT5G49520 52.055 73 34 1 150 222 206 277 6.83e-18 85.5
MS.gene74841.t1 AT2G30250 52.055 73 34 1 150 222 313 384 8.55e-18 85.5
MS.gene74841.t1 AT2G47260 49.315 73 36 1 150 222 159 230 9.45e-18 84.7
MS.gene74841.t1 AT1G29860 49.315 73 36 1 150 222 121 192 2.52e-17 82.4
MS.gene74841.t1 AT5G26170 43.243 74 41 1 152 225 100 172 2.78e-17 79.7
MS.gene74841.t1 AT4G39410 49.333 75 37 1 150 224 208 281 3.14e-17 82.4
MS.gene74841.t1 AT4G18170 47.945 73 37 1 150 222 157 228 2.21e-16 80.1
MS.gene74841.t1 AT5G13080 48.611 72 36 1 151 222 53 123 2.86e-16 76.3
MS.gene74841.t1 AT1G69310 45.946 74 39 1 152 225 134 206 4.56e-16 79.0
MS.gene74841.t1 AT1G69310 45.946 74 39 1 152 225 134 206 4.56e-16 79.0
MS.gene74841.t1 AT1G69310 45.946 74 39 1 152 225 134 206 4.56e-16 79.0
MS.gene74841.t1 AT3G62340 52.113 71 33 1 152 222 105 174 6.03e-16 78.2
MS.gene74841.t1 AT5G46350 45.070 71 38 1 152 222 170 239 2.06e-15 77.4
MS.gene74841.t1 AT2G46130 47.887 71 32 1 152 222 17 82 2.12e-15 72.4
MS.gene74841.t1 AT3G01970 48.052 77 37 2 152 228 52 125 2.76e-15 73.2
MS.gene74841.t1 AT2G21900 51.562 64 29 2 160 222 103 165 3.65e-15 74.3
MS.gene74841.t1 AT4G31550 37.903 124 58 5 120 242 211 316 7.68e-15 75.9
MS.gene74841.t1 AT1G69310 41.892 74 38 1 152 225 134 202 7.09e-14 72.4
MS.gene74841.t1 AT4G30935 39.252 107 55 3 128 230 295 395 1.97e-13 72.4
MS.gene74841.t1 AT2G37260 49.206 63 31 1 160 222 344 405 1.99e-13 72.4
MS.gene74841.t1 AT2G37260 49.206 63 31 1 160 222 264 325 2.37e-13 71.6
MS.gene74841.t1 AT2G37260 49.206 63 31 1 160 222 262 323 2.63e-13 71.2
MS.gene74841.t1 AT4G31550 37.600 125 58 6 120 242 211 317 2.64e-13 71.2
MS.gene74841.t1 AT4G01250 41.000 100 43 4 124 222 101 185 5.34e-13 69.7
MS.gene74841.t1 AT4G23550 43.243 74 40 2 150 222 125 197 9.47e-13 69.3
MS.gene74841.t1 AT4G04450 44.444 72 38 2 152 222 185 255 1.24e-12 69.7
MS.gene74841.t1 AT5G52830 39.623 106 56 3 150 254 150 248 1.62e-12 68.9
MS.gene74841.t1 AT2G46400 41.667 84 45 2 152 232 91 173 2.78e-12 67.8
MS.gene74841.t1 AT4G22070 43.056 72 39 2 152 222 284 354 3.30e-12 68.9
MS.gene74841.t1 AT4G22070 43.056 72 39 2 152 222 284 354 3.30e-12 68.9
MS.gene74841.t1 AT4G22070 43.056 72 39 2 152 222 284 354 3.30e-12 68.9
MS.gene74841.t1 AT4G22070 43.056 72 39 2 152 222 284 354 3.30e-12 68.9
MS.gene74841.t1 AT1G29280 43.011 93 48 3 131 222 43 131 3.51e-12 67.0
MS.gene74841.t1 AT2G25000 34.862 109 55 5 131 224 56 163 3.55e-12 66.2
MS.gene74841.t1 AT2G25000 34.862 109 55 5 131 224 56 163 3.55e-12 66.2
MS.gene74841.t1 AT4G04450 44.444 72 38 2 152 222 279 349 3.61e-12 68.6
MS.gene74841.t1 AT2G25000 34.862 109 55 5 131 224 78 185 5.09e-12 66.2
MS.gene74841.t1 AT2G23320 36.111 108 49 4 121 223 206 298 5.43e-12 67.0
MS.gene74841.t1 AT5G43290 43.056 72 39 2 152 222 100 170 5.61e-12 66.6
MS.gene74841.t1 AT4G24240 44.444 81 39 3 152 227 264 343 5.95e-12 67.4
MS.gene74841.t1 AT4G01720 44.444 72 38 2 152 222 226 296 7.19e-12 67.8
MS.gene74841.t1 AT5G15130 43.056 72 39 2 152 222 172 242 7.24e-12 67.8
MS.gene74841.t1 AT2G25000 34.862 109 55 5 131 224 99 206 8.48e-12 65.9
MS.gene74841.t1 AT5G15130 43.056 72 39 2 152 222 214 284 9.35e-12 67.4
MS.gene74841.t1 AT1G62300 43.056 72 39 2 152 222 299 369 9.59e-12 67.4
MS.gene74841.t1 AT2G24570 40.000 105 49 5 120 222 208 300 2.09e-11 65.5
MS.gene74841.t1 AT2G34830 47.692 65 32 2 159 222 209 272 6.10e-11 64.7
MS.gene74841.t1 AT2G34830 47.692 65 32 2 159 222 218 281 7.02e-11 64.3

Find 125 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCAAGTCTTTCCTTTCA+TGG 0.213204 1.1:+58700766 MS.gene74841:CDS
TGAAAGTGATGAAGACATTT+TGG 0.222211 1.1:+58699261 MS.gene74841:CDS
ATCAGTTGTTCTATCTTCTT+TGG 0.239892 1.1:-58698908 None:intergenic
CAAGTTGGACCTAATATTAA+TGG 0.252073 1.1:+58700638 MS.gene74841:CDS
AATAATTACCTACGATTGTT+AGG 0.259815 1.1:-58699334 None:intergenic
GGAATTGGCCCGTTGATATT+TGG 0.273233 1.1:-58700473 None:intergenic
AATGAATCTCCTCCTTCTTC+TGG 0.277307 1.1:+58697543 None:intergenic
GAAATTGACCCATTAATATT+AGG 0.309554 1.1:-58700647 None:intergenic
TATGTAACAAGTACATATTT+TGG 0.310515 1.1:-58700125 None:intergenic
CCAACTTGAGTTGTGGTAAT+AGG 0.312353 1.1:-58700623 None:intergenic
TTTCTTCAGGTTGGTGGAAA+TGG 0.328210 1.1:+58700734 MS.gene74841:CDS
AAAGGCCGCGTCATGGTATT+AGG 0.337576 1.1:-58700344 None:intergenic
TTACCCACCATGTTTAATAA+AGG 0.347175 1.1:-58700362 None:intergenic
GGGTGCTTCCCCAGCTGATC+AGG 0.355314 1.1:+58700787 MS.gene74841:CDS
GTCCTTGATCAGGTGGTGTT+GGG 0.367401 1.1:-58698765 None:intergenic
AACTCCTATTGATACAAATC+AGG 0.367991 1.1:+58700445 MS.gene74841:CDS
ATACCTTATGGTATGAATTA+CGG 0.368502 1.1:+58700317 MS.gene74841:CDS
CAATATGGCTATATACCTTA+TGG 0.368992 1.1:+58700305 MS.gene74841:CDS
CATAAGGTATATAGCCATAT+TGG 0.374743 1.1:-58700304 None:intergenic
GCTCAAGTCTTTCCTTTCAT+GGG 0.380582 1.1:+58700767 MS.gene74841:CDS
TCGAGAAATATTAATGCATC+TGG 0.384031 1.1:+58700200 MS.gene74841:CDS
GGAAATACAGGAAGCGGAAT+TGG 0.386630 1.1:-58700488 None:intergenic
CCTAAATATCATTCGGTGTC+AGG 0.391994 1.1:-58700824 None:intergenic
TGCAATTCAGGAAGAGAAAG+AGG 0.396225 1.1:+58699170 MS.gene74841:intron
CCATTTCACATTGTACCATA+TGG 0.401565 1.1:+58700704 MS.gene74841:CDS
TATGTACTTGTTACATATGA+TGG 0.402941 1.1:+58700131 MS.gene74841:CDS
CCAACTTGAGTTGCAGTCAT+AGG 0.404757 1.1:-58700536 None:intergenic
TGTTCACTTATGGGTCTGTA+AGG 0.409849 1.1:-58700398 None:intergenic
TCTGCACCATGTCCTTGATC+AGG 0.410286 1.1:-58698775 None:intergenic
GGTGGTAATAGTGGTGATAT+CGG 0.419782 1.1:-58700683 None:intergenic
CAAGTTGGACATAATATCAA+TGG 0.426656 1.1:+58700551 MS.gene74841:CDS
CCAACCTGATTTGTATCAAT+AGG 0.427285 1.1:-58700449 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1.1:-58700509 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1.1:-58700596 None:intergenic
TCCGTAGCTTCCTGATCAGC+TGG 0.428219 1.1:-58700797 None:intergenic
AAGTGATTATAAAACCTTTG+TGG 0.432121 1.1:-58699054 None:intergenic
TATAGCCATATTGGTGAAAA+GGG 0.437091 1.1:-58700295 None:intergenic
ATATAGCCATATTGGTGAAA+AGG 0.444538 1.1:-58700296 None:intergenic
AAGTTGGACCTAATATTAAT+GGG 0.454569 1.1:+58700639 MS.gene74841:CDS
GGTGGTGATATCGGAAATAC+AGG 0.454748 1.1:-58700500 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1.1:-58700587 None:intergenic
AGTGGTGATATCGGAAATAC+AGG 0.457187 1.1:-58700674 None:intergenic
AATGCAGGTGGTGGTGATGG+TGG 0.472619 1.1:-58700251 None:intergenic
TGAGTTGCAGTCATAGGAAA+TGG 0.474839 1.1:-58700530 None:intergenic
ACTGCTTCAGAAAAGTTCAT+TGG 0.480463 1.1:-58698880 None:intergenic
CAACCGTAATTCATACCATA+AGG 0.481218 1.1:-58700320 None:intergenic
CCTATGACTGCAACTCAAGT+TGG 0.482132 1.1:+58700536 MS.gene74841:CDS
TGTCCTTGATCAGGTGGTGT+TGG 0.482577 1.1:-58698766 None:intergenic
GTAATAGGAAATGGTGGTAA+TGG 0.484085 1.1:-58700608 None:intergenic
CCTATTACCACAACTCAAGT+TGG 0.484916 1.1:+58700623 MS.gene74841:CDS
GCAGGTGGTGGTGATGGTGG+TGG 0.485365 1.1:-58700248 None:intergenic
TGAAGAAAATCCCCACCATA+TGG 0.492962 1.1:-58700719 None:intergenic
ACCACCACCTGCATTGCCTA+TGG 0.498003 1.1:+58700259 MS.gene74841:CDS
TTAATATTTCTCGAGTATGT+TGG 0.504174 1.1:-58700191 None:intergenic
GATGATGAAGCAACTGCCAT+TGG 0.509238 1.1:+58698982 MS.gene74841:CDS
TATGTAGCCTAAATATCATT+CGG 0.509419 1.1:-58700831 None:intergenic
GGGTGAACCATAGGCAATGC+AGG 0.510944 1.1:-58700266 None:intergenic
GGCAATGCAGGTGGTGGTGA+TGG 0.511381 1.1:-58700254 None:intergenic
AGGTTGGACCAAATATCAAC+GGG 0.512849 1.1:+58700465 MS.gene74841:CDS
TTCACATTGTACCATATGGT+GGG 0.526608 1.1:+58700708 MS.gene74841:CDS
CCATATGGTACAATGTGAAA+TGG 0.527122 1.1:-58700704 None:intergenic
GCTGGGGAAGCACCCATGAA+AGG 0.527399 1.1:-58700779 None:intergenic
CCCAGCTGATCAGGAAGCTA+CGG 0.531217 1.1:+58700796 MS.gene74841:CDS
TGAGTTGTGGTAATAGGAAA+TGG 0.532382 1.1:-58700617 None:intergenic
ATGTGAAATGGTGGTAATAG+TGG 0.532968 1.1:-58700692 None:intergenic
AAGTTGGACATAATATCAAT+GGG 0.540013 1.1:+58700552 MS.gene74841:CDS
TCCCCAACACCACCTGATCA+AGG 0.540123 1.1:+58698763 MS.gene74841:CDS
TATGATGATGGTGGTGCTAG+TGG 0.544707 1.1:+58699024 MS.gene74841:CDS
TCGACATCTGTCATGGGGCA+AGG 0.545512 1.1:-58699217 None:intergenic
GTCATAGGAAATGGTGGTAA+TGG 0.551917 1.1:-58700521 None:intergenic
GATGAAGCAACTGCCATTGG+AGG 0.552119 1.1:+58698985 MS.gene74841:CDS
GGTTATTGTTGGCGCAAGTA+CGG 0.553147 1.1:+58699289 MS.gene74841:CDS
AAATTCTAAGGGCAAGGAAA+GGG 0.559579 1.1:+58698851 MS.gene74841:CDS
TCACATTGTACCATATGGTG+GGG 0.559672 1.1:+58700709 MS.gene74841:CDS
GAAATTCTAAGGGCAAGGAA+AGG 0.560612 1.1:+58698850 MS.gene74841:CDS
ATGCTGAAATTCTAAGGGCA+AGG 0.561694 1.1:+58698845 MS.gene74841:CDS
CCGTAGCTTCCTGATCAGCT+GGG 0.562306 1.1:-58700796 None:intergenic
TCCTTCTTCTGGTTATTCCG+AGG 0.562652 1.1:+58697554 MS.gene74841:CDS
ACCTCGGAATAACCAGAAGA+AGG 0.563675 1.1:-58697555 None:intergenic
GATATCGGAAATACAGGAAG+CGG 0.565608 1.1:-58700494 None:intergenic
ATAGGAAATGGTGGTAATGG+TGG 0.566411 1.1:-58700605 None:intergenic
CAGCAACATCATTCCTCCAA+TGG 0.568839 1.1:-58698998 None:intergenic
TTTGTCAATGACAGGTCCGT+GGG 0.568911 1.1:-58700162 None:intergenic
TTTCACATTGTACCATATGG+TGG 0.572755 1.1:+58700707 MS.gene74841:CDS
GATATCGGAAATACAGGAAG+CGG 0.574632 1.1:-58700581 None:intergenic
CCTATTGATACAAATCAGGT+TGG 0.576330 1.1:+58700449 MS.gene74841:CDS
GCAATTCAGGAAGAGAAAGA+GGG 0.576835 1.1:+58699171 MS.gene74841:intron
TCCTTGATCAGGTGGTGTTG+GGG 0.585344 1.1:-58698764 None:intergenic
GTTTAATAAAGGCCGCGTCA+TGG 0.585854 1.1:-58700351 None:intergenic
GCAGTTGCTTCATCATCCGC+AGG 0.586772 1.1:-58698976 None:intergenic
ATTAGGTCCAACTTGAGTTG+TGG 0.589594 1.1:-58700630 None:intergenic
TGTCTTCATCACTTTCAACT+CGG 0.590497 1.1:-58699255 None:intergenic
CTTATGGTATGAATTACGGT+TGG 0.590836 1.1:+58700321 MS.gene74841:CDS
TGATGGTGGTGGCAGCATGT+TGG 0.595809 1.1:-58700237 None:intergenic
CGGCCTTTATTAAACATGGT+GGG 0.600743 1.1:+58700359 MS.gene74841:CDS
CAGGTTGGACCAAATATCAA+CGG 0.601960 1.1:+58700464 MS.gene74841:CDS
ACCATAGGCAATGCAGGTGG+TGG 0.608469 1.1:-58700260 None:intergenic
TACGGATGCAAAGAGATCAA+AGG 0.609552 1.1:+58699307 MS.gene74841:CDS
GCACCATGTCCTTGATCAGG+TGG 0.613784 1.1:-58698772 None:intergenic
GACGCGGCCTTTATTAAACA+TGG 0.614916 1.1:+58700355 MS.gene74841:CDS
TCTGAAACTTGATCATCAGT+TGG 0.615285 1.1:-58698949 None:intergenic
AAGGAAATCCTAACAATCGT+AGG 0.617006 1.1:+58699326 MS.gene74841:CDS
TTTGCTCGACATCTGTCATG+GGG 0.618862 1.1:-58699222 None:intergenic
TAGCCATATTGGTGAAAAGG+GGG 0.626106 1.1:-58700293 None:intergenic
ATGTTGCTGCAATATGATGA+TGG 0.628176 1.1:+58699012 MS.gene74841:CDS
ATAGGAAATGGTGGTAATGG+TGG 0.629683 1.1:-58700518 None:intergenic
GCGGCCTTTATTAAACATGG+TGG 0.630164 1.1:+58700358 MS.gene74841:CDS
CGTAGCTTCCTGATCAGCTG+GGG 0.653265 1.1:-58700795 None:intergenic
TATGATGGAAAACATACCCA+CGG 0.656713 1.1:+58700146 MS.gene74841:CDS
GGGTCTGTAAGGTTGCATCA+TGG 0.657757 1.1:-58700387 None:intergenic
GTTGCAGTCATAGGAAATGG+TGG 0.663032 1.1:-58700527 None:intergenic
GCAAAATGATGCAAATGTAG+TGG 0.669148 1.1:+58698816 MS.gene74841:CDS
ATAGCCATATTGGTGAAAAG+GGG 0.680391 1.1:-58700294 None:intergenic
CTAGTGGATCTTGTCCACAA+AGG 0.681150 1.1:+58699040 MS.gene74841:CDS
ACATCTGTCATGGGGCAAGG+TGG 0.684322 1.1:-58699214 None:intergenic
GGGGATGAGAAATTCAACAC+TGG 0.684419 1.1:-58698745 None:intergenic
GTTGTGGTAATAGGAAATGG+TGG 0.688257 1.1:-58700614 None:intergenic
TCGGAATAACCAGAAGAAGG+AGG 0.688798 1.1:-58697552 None:intergenic
TATGGTACAATGTGAAATGG+TGG 0.694153 1.1:-58700701 None:intergenic
ACACCACCTGATCAAGGACA+TGG 0.702867 1.1:+58698769 MS.gene74841:CDS
TGAACCATAGGCAATGCAGG+TGG 0.713852 1.1:-58700263 None:intergenic
AGCCATATTGGTGAAAAGGG+GGG 0.719943 1.1:-58700292 None:intergenic
TTGCTGCAATATGATGATGG+TGG 0.726113 1.1:+58699015 MS.gene74841:CDS
TCAAGTTTCAGATACACCTG+CGG 0.763156 1.1:+58698960 MS.gene74841:CDS
GTTGGCCTAATACCATGACG+CGG 0.807585 1.1:+58700339 MS.gene74841:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGACATTATTTAAAAAAAAA+GGG - chr1.1:58698118-58698137 None:intergenic 10.0%
!!! TTTTCATCAAAAAATAAAAA+AGG - chr1.1:58697809-58697828 None:intergenic 10.0%
!! AGAATTTGATATTAAATTTG+TGG + chr1.1:58699794-58699813 MS.gene74841:intron 15.0%
!! CAAATTTAATATCAAATTCT+AGG - chr1.1:58699794-58699813 None:intergenic 15.0%
!! GTGACATTATTTAAAAAAAA+AGG - chr1.1:58698119-58698138 None:intergenic 15.0%
!! TACTAATACATTTATGTAAA+TGG - chr1.1:58699968-58699987 None:intergenic 15.0%
!! TCACACAATATTAAATAATT+AGG - chr1.1:58700031-58700050 None:intergenic 15.0%
!! TTGTAAAAATATCAAAAACA+AGG - chr1.1:58699637-58699656 None:intergenic 15.0%
!!! CTTTATCATTTTATTTTAAG+CGG + chr1.1:58699864-58699883 MS.gene74841:intron 15.0%
!!! TTTTGTTTTTATAGTTTCAT+AGG + chr1.1:58698473-58698492 MS.gene74841:intron 15.0%
!! ACATTGTTGCTAATAAATAA+AGG + chr1.1:58699122-58699141 MS.gene74841:intron 20.0%
!! ATAAATAAATCATTCCATGT+TGG - chr1.1:58698527-58698546 None:intergenic 20.0%
!! ATATTCCTAATGAGAAATAA+TGG - chr1.1:58698270-58698289 None:intergenic 20.0%
!! GTAATTGAATCAAATTCATT+AGG + chr1.1:58698651-58698670 MS.gene74841:intron 20.0%
!! TACATGTTAGATTGAATTAA+AGG - chr1.1:58698369-58698388 None:intergenic 20.0%
!! TCTTCATTCAAGATTTAAAA+TGG - chr1.1:58698080-58698099 None:intergenic 20.0%
!! TTAATATTGTGTGAATTGTA+GGG + chr1.1:58700037-58700056 MS.gene74841:intron 20.0%
!! TTTAATATTGTGTGAATTGT+AGG + chr1.1:58700036-58700055 MS.gene74841:intron 20.0%
!!! AAAAGAGTATATAAATACCT+CGG - chr1.1:58697574-58697593 None:intergenic 20.0%
!!! AATGGCTTTTCAATTAAATT+CGG - chr1.1:58698062-58698081 None:intergenic 20.0%
!!! AGAAAATTTTGTTCACTTAT+GGG - chr1.1:58700410-58700429 None:intergenic 20.0%
!!! TAGAAAATTTTGTTCACTTA+TGG - chr1.1:58700411-58700430 None:intergenic 20.0%
!!! TATGTAACAAGTACATATTT+TGG - chr1.1:58700128-58700147 None:intergenic 20.0%
!!! TTTTACGATAAATCACTTAT+CGG - chr1.1:58699767-58699786 None:intergenic 20.0%
! AAGTGATTATAAAACCTTTG+TGG - chr1.1:58699057-58699076 None:intergenic 25.0%
! AAGTTGGACATAATATCAAT+GGG + chr1.1:58700552-58700571 MS.gene74841:CDS 25.0%
! AAGTTGGACCTAATATTAAT+GGG + chr1.1:58700639-58700658 MS.gene74841:CDS 25.0%
! AAGTTGTATTAGTGCTAAAA+TGG - chr1.1:58698302-58698321 None:intergenic 25.0%
! AATAATTACCTACGATTGTT+AGG - chr1.1:58699337-58699356 None:intergenic 25.0%
! AATCTAACAATGATTGATGT+TGG - chr1.1:58699704-58699723 None:intergenic 25.0%
! AATGACATAATGATCATTGA+CGG + chr1.1:58699575-58699594 MS.gene74841:intron 25.0%
! ACATTAAAAAACAAGTGGAA+AGG + chr1.1:58700090-58700109 MS.gene74841:CDS 25.0%
! ATACCTTATGGTATGAATTA+CGG + chr1.1:58700317-58700336 MS.gene74841:CDS 25.0%
! ATTGAATTAAAGGTCTGATT+AGG - chr1.1:58698359-58698378 None:intergenic 25.0%
! CATTAAAAAACAAGTGGAAA+GGG + chr1.1:58700091-58700110 MS.gene74841:CDS 25.0%
! GAAAAATGCTGAAATTCTAA+GGG + chr1.1:58698840-58698859 MS.gene74841:CDS 25.0%
! GAAATTGACCCATTAATATT+AGG - chr1.1:58700650-58700669 None:intergenic 25.0%
! GAATTTGGATTCGTTTATAT+TGG - chr1.1:58698191-58698210 None:intergenic 25.0%
! TAATATTGTGTGAATTGTAG+GGG + chr1.1:58700038-58700057 MS.gene74841:intron 25.0%
! TGAAAAATGCTGAAATTCTA+AGG + chr1.1:58698839-58698858 MS.gene74841:CDS 25.0%
! TGCATTAACACATATAAAAG+AGG - chr1.1:58699362-58699381 None:intergenic 25.0%
! TTAATATTTCTCGAGTATGT+TGG - chr1.1:58700194-58700213 None:intergenic 25.0%
! TTGTGACATTAAAAAACAAG+TGG + chr1.1:58700085-58700104 MS.gene74841:CDS 25.0%
!! TATGTACTTGTTACATATGA+TGG + chr1.1:58700131-58700150 MS.gene74841:CDS 25.0%
!!! AGAGATGATTTTCAAGTATT+GGG + chr1.1:58699725-58699744 MS.gene74841:intron 25.0%
!!! TAGAGATGATTTTCAAGTAT+TGG + chr1.1:58699724-58699743 MS.gene74841:intron 25.0%
AACTCCTATTGATACAAATC+AGG + chr1.1:58700445-58700464 MS.gene74841:CDS 30.0%
AATATTGTGTGAATTGTAGG+GGG + chr1.1:58700039-58700058 MS.gene74841:intron 30.0%
ATATAGCCATATTGGTGAAA+AGG - chr1.1:58700299-58700318 None:intergenic 30.0%
ATCAGTTGTTCTATCTTCTT+TGG - chr1.1:58698911-58698930 None:intergenic 30.0%
CAATATGGCTATATACCTTA+TGG + chr1.1:58700305-58700324 MS.gene74841:CDS 30.0%
CATAAGGTATATAGCCATAT+TGG - chr1.1:58700307-58700326 None:intergenic 30.0%
GGTGAAAAATGATGTGAATT+TGG - chr1.1:58698206-58698225 None:intergenic 30.0%
GTCAATGATCATTATGTCAT+TGG - chr1.1:58699576-58699595 None:intergenic 30.0%
TATAGCCATATTGGTGAAAA+GGG - chr1.1:58700298-58700317 None:intergenic 30.0%
TCGAGAAATATTAATGCATC+TGG + chr1.1:58700200-58700219 MS.gene74841:CDS 30.0%
TTACCCACCATGTTTAATAA+AGG - chr1.1:58700365-58700384 None:intergenic 30.0%
! ACTTTTAATAACCACACAAG+CGG - chr1.1:58698232-58698251 None:intergenic 30.0%
! ATGCACCATTATTTCTCATT+AGG + chr1.1:58698262-58698281 MS.gene74841:intron 30.0%
! CAAGTTGGACATAATATCAA+TGG + chr1.1:58700551-58700570 MS.gene74841:CDS 30.0%
! CAAGTTGGACCTAATATTAA+TGG + chr1.1:58700638-58700657 MS.gene74841:CDS 30.0%
! TGAAAGTGATGAAGACATTT+TGG + chr1.1:58699261-58699280 MS.gene74841:CDS 30.0%
!! CAAATAGTTTTACACCAACA+TGG + chr1.1:58698510-58698529 MS.gene74841:intron 30.0%
!! GCTCAATTATTTTGCAATTC+AGG + chr1.1:58699158-58699177 MS.gene74841:intron 30.0%
!! TTTTCTTTTTCATTCACTCC+TGG + chr1.1:58699405-58699424 MS.gene74841:intron 30.0%
!! TTTTGGATGATGGTTATTGT+TGG + chr1.1:58699278-58699297 MS.gene74841:CDS 30.0%
AAGGAAATCCTAACAATCGT+AGG + chr1.1:58699326-58699345 MS.gene74841:CDS 35.0%
ACAACAGTGTTCATCATCAT+TGG - chr1.1:58699507-58699526 None:intergenic 35.0%
ACTGCTTCAGAAAAGTTCAT+TGG - chr1.1:58698883-58698902 None:intergenic 35.0%
ATAGCCATATTGGTGAAAAG+GGG - chr1.1:58700297-58700316 None:intergenic 35.0%
ATGTGAAATGGTGGTAATAG+TGG - chr1.1:58700695-58700714 None:intergenic 35.0%
ATGTTGCTGCAATATGATGA+TGG + chr1.1:58699012-58699031 MS.gene74841:CDS 35.0%
CAACCGTAATTCATACCATA+AGG - chr1.1:58700323-58700342 None:intergenic 35.0%
CCATATGGTACAATGTGAAA+TGG - chr1.1:58700707-58700726 None:intergenic 35.0%
CCATTTCACATTGTACCATA+TGG + chr1.1:58700704-58700723 MS.gene74841:CDS 35.0%
CCTATTGATACAAATCAGGT+TGG + chr1.1:58700449-58700468 MS.gene74841:CDS 35.0%
CTTATGGTATGAATTACGGT+TGG + chr1.1:58700321-58700340 MS.gene74841:CDS 35.0%
GCAAAATGATGCAAATGTAG+TGG + chr1.1:58698816-58698835 MS.gene74841:CDS 35.0%
GTAATAGGAAATGGTGGTAA+TGG - chr1.1:58700611-58700630 None:intergenic 35.0%
TATGGTACAATGTGAAATGG+TGG - chr1.1:58700704-58700723 None:intergenic 35.0%
TCCTCTCTATATATACACCA+AGG - chr1.1:58699431-58699450 None:intergenic 35.0%
TGAGTTGTGGTAATAGGAAA+TGG - chr1.1:58700620-58700639 None:intergenic 35.0%
TGTCTTCATCACTTTCAACT+CGG - chr1.1:58699258-58699277 None:intergenic 35.0%
TTCACATTGTACCATATGGT+GGG + chr1.1:58700708-58700727 MS.gene74841:CDS 35.0%
TTGGTGTATATATAGAGAGG+AGG + chr1.1:58699430-58699449 MS.gene74841:intron 35.0%
TTTCACATTGTACCATATGG+TGG + chr1.1:58700707-58700726 MS.gene74841:CDS 35.0%
! AAATTCTAAGGGCAAGGAAA+GGG + chr1.1:58698851-58698870 MS.gene74841:CDS 35.0%
! CATTTGCATCATTTTGCACA+TGG - chr1.1:58698813-58698832 None:intergenic 35.0%
! CCAACCTGATTTGTATCAAT+AGG - chr1.1:58700452-58700471 None:intergenic 35.0%
! GATGAAGACATTTTGGATGA+TGG + chr1.1:58699268-58699287 MS.gene74841:CDS 35.0%
! TATGATGGAAAACATACCCA+CGG + chr1.1:58700146-58700165 MS.gene74841:CDS 35.0%
! TCTGAAACTTGATCATCAGT+TGG - chr1.1:58698952-58698971 None:intergenic 35.0%
!! TGATTGATGTTGGTTCAACA+CGG - chr1.1:58699694-58699713 None:intergenic 35.0%
!! TTATGTCATTGGCTTAGTTG+TGG - chr1.1:58699565-58699584 None:intergenic 35.0%
!!! GGTCTTTTTTTGTCAATGAC+AGG - chr1.1:58700173-58700192 None:intergenic 35.0%
AAATGAACCAGTAGCAACCA+CGG - chr1.1:58697986-58698005 None:intergenic 40.0%
AGGTTGGACCAAATATCAAC+GGG + chr1.1:58700465-58700484 MS.gene74841:CDS 40.0%
AGTGGTGATATCGGAAATAC+AGG - chr1.1:58700677-58700696 None:intergenic 40.0%
CCTATTACCACAACTCAAGT+TGG + chr1.1:58700623-58700642 MS.gene74841:CDS 40.0%
CGGCCTTTATTAAACATGGT+GGG + chr1.1:58700359-58700378 MS.gene74841:CDS 40.0%
GATATCGGAAATACAGGAAG+CGG - chr1.1:58700497-58700516 None:intergenic 40.0%
GCAATTCAGGAAGAGAAAGA+GGG + chr1.1:58699171-58699190 MS.gene74841:intron 40.0%
GCTCAAGTCTTTCCTTTCAT+GGG + chr1.1:58700767-58700786 MS.gene74841:CDS 40.0%
GGTGGTAATAGTGGTGATAT+CGG - chr1.1:58700686-58700705 None:intergenic 40.0%
GTCATAGGAAATGGTGGTAA+TGG - chr1.1:58700524-58700543 None:intergenic 40.0%
GTTGTGGTAATAGGAAATGG+TGG - chr1.1:58700617-58700636 None:intergenic 40.0%
TACGGATGCAAAGAGATCAA+AGG + chr1.1:58699307-58699326 MS.gene74841:CDS 40.0%
TAGCCATATTGGTGAAAAGG+GGG - chr1.1:58700296-58700315 None:intergenic 40.0%
TCAAGTTTCAGATACACCTG+CGG + chr1.1:58698960-58698979 MS.gene74841:CDS 40.0%
TCACATTGTACCATATGGTG+GGG + chr1.1:58700709-58700728 MS.gene74841:CDS 40.0%
TCTATATATACACCAAGGCC+AGG - chr1.1:58699426-58699445 None:intergenic 40.0%
TGAAGAAAATCCCCACCATA+TGG - chr1.1:58700722-58700741 None:intergenic 40.0%
TGACGGTTTGACGAAAACAT+GGG + chr1.1:58699592-58699611 MS.gene74841:intron 40.0%
TGAGTTGCAGTCATAGGAAA+TGG - chr1.1:58700533-58700552 None:intergenic 40.0%
TGCAATTCAGGAAGAGAAAG+AGG + chr1.1:58699170-58699189 MS.gene74841:intron 40.0%
TGCTCAAGTCTTTCCTTTCA+TGG + chr1.1:58700766-58700785 MS.gene74841:CDS 40.0%
TGTTCACTTATGGGTCTGTA+AGG - chr1.1:58700401-58700420 None:intergenic 40.0%
TTGACGGTTTGACGAAAACA+TGG + chr1.1:58699591-58699610 MS.gene74841:intron 40.0%
! ATGCTGAAATTCTAAGGGCA+AGG + chr1.1:58698845-58698864 MS.gene74841:CDS 40.0%
! ATTAGGTCCAACTTGAGTTG+TGG - chr1.1:58700633-58700652 None:intergenic 40.0%
! CAGGTTGGACCAAATATCAA+CGG + chr1.1:58700464-58700483 MS.gene74841:CDS 40.0%
! CCAACTTGAGTTGTGGTAAT+AGG - chr1.1:58700626-58700645 None:intergenic 40.0%
! GAAATTCTAAGGGCAAGGAA+AGG + chr1.1:58698850-58698869 MS.gene74841:CDS 40.0%
! GCCTTGGTGTATATATAGAG+AGG + chr1.1:58699427-58699446 MS.gene74841:intron 40.0%
! TTTTGCTCGACATCTGTCAT+GGG - chr1.1:58699226-58699245 None:intergenic 40.0%
! TTTTTGCTCGACATCTGTCA+TGG - chr1.1:58699227-58699246 None:intergenic 40.0%
!! ATAGGAAATGGTGGTAATGG+TGG - chr1.1:58700521-58700540 None:intergenic 40.0%
!! ATATGGTGGGGATTTTCTTC+AGG + chr1.1:58700721-58700740 MS.gene74841:CDS 40.0%
!! TTGCTGCAATATGATGATGG+TGG + chr1.1:58699015-58699034 MS.gene74841:CDS 40.0%
!! TTTCTTCAGGTTGGTGGAAA+TGG + chr1.1:58700734-58700753 MS.gene74841:CDS 40.0%
ACCTCGGAATAACCAGAAGA+AGG - chr1.1:58697558-58697577 None:intergenic 45.0%
AGCCATATTGGTGAAAAGGG+GGG - chr1.1:58700295-58700314 None:intergenic 45.0%
CAGCAACATCATTCCTCCAA+TGG - chr1.1:58699001-58699020 None:intergenic 45.0%
CCTATGACTGCAACTCAAGT+TGG + chr1.1:58700536-58700555 MS.gene74841:CDS 45.0%
GACGCGGCCTTTATTAAACA+TGG + chr1.1:58700355-58700374 MS.gene74841:CDS 45.0%
GATGATGAAGCAACTGCCAT+TGG + chr1.1:58698982-58699001 MS.gene74841:CDS 45.0%
GCGGCCTTTATTAAACATGG+TGG + chr1.1:58700358-58700377 MS.gene74841:CDS 45.0%
GGAAATACAGGAAGCGGAAT+TGG - chr1.1:58700491-58700510 None:intergenic 45.0%
GGAATTGGCCCGTTGATATT+TGG - chr1.1:58700476-58700495 None:intergenic 45.0%
GGGGATGAGAAATTCAACAC+TGG - chr1.1:58698748-58698767 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1.1:58700503-58700522 None:intergenic 45.0%
GGTTATTGTTGGCGCAAGTA+CGG + chr1.1:58699289-58699308 MS.gene74841:CDS 45.0%
GTTGCAGTCATAGGAAATGG+TGG - chr1.1:58700530-58700549 None:intergenic 45.0%
GTTTAATAAAGGCCGCGTCA+TGG - chr1.1:58700354-58700373 None:intergenic 45.0%
TAATAACCACACAAGCGGAG+AGG - chr1.1:58698227-58698246 None:intergenic 45.0%
TCGGAATAACCAGAAGAAGG+AGG - chr1.1:58697555-58697574 None:intergenic 45.0%
TTCTGCTGATGATTGCCATG+TGG + chr1.1:58698675-58698694 MS.gene74841:intron 45.0%
TTTGCTCGACATCTGTCATG+GGG - chr1.1:58699225-58699244 None:intergenic 45.0%
TTTGTCAATGACAGGTCCGT+GGG - chr1.1:58700165-58700184 None:intergenic 45.0%
! CCAACTTGAGTTGCAGTCAT+AGG - chr1.1:58700539-58700558 None:intergenic 45.0%
! CTAGTGGATCTTGTCCACAA+AGG + chr1.1:58699040-58699059 MS.gene74841:CDS 45.0%
! TCCTTCTTCTGGTTATTCCG+AGG + chr1.1:58697554-58697573 MS.gene74841:CDS 45.0%
! TTTTCATTCACTCCTGGCCT+TGG + chr1.1:58699411-58699430 MS.gene74841:intron 45.0%
! TTTTGTCAATGACAGGTCCG+TGG - chr1.1:58700166-58700185 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1.1:58700512-58700531 None:intergenic 45.0%
!! TATGATGATGGTGGTGCTAG+TGG + chr1.1:58699024-58699043 MS.gene74841:CDS 45.0%
AAAGGCCGCGTCATGGTATT+AGG - chr1.1:58700347-58700366 None:intergenic 50.0%
ACACCACCTGATCAAGGACA+TGG + chr1.1:58698769-58698788 MS.gene74841:CDS 50.0%
ATTGGTGAAAAGGGGGGAGA+GGG - chr1.1:58700289-58700308 None:intergenic 50.0%
CATCTCCTCTTGTTGCCACA+TGG - chr1.1:58698693-58698712 None:intergenic 50.0%
GATGAAGCAACTGCCATTGG+AGG + chr1.1:58698985-58699004 MS.gene74841:CDS 50.0%
GATTGCCATGTGGCAACAAG+AGG + chr1.1:58698685-58698704 MS.gene74841:intron 50.0%
GGGTCTGTAAGGTTGCATCA+TGG - chr1.1:58700390-58700409 None:intergenic 50.0%
GTCCTTGATCAGGTGGTGTT+GGG - chr1.1:58698768-58698787 None:intergenic 50.0%
GTCGTGTGTGCATGCATACA+CGG + chr1.1:58697960-58697979 MS.gene74841:intron 50.0%
TATTGGTGAAAAGGGGGGAG+AGG - chr1.1:58700290-58700309 None:intergenic 50.0%
TCTGCACCATGTCCTTGATC+AGG - chr1.1:58698778-58698797 None:intergenic 50.0%
TGAACCATAGGCAATGCAGG+TGG - chr1.1:58700266-58700285 None:intergenic 50.0%
TGTCCTTGATCAGGTGGTGT+TGG - chr1.1:58698769-58698788 None:intergenic 50.0%
! CTCCCCCCTTTTCACCAATA+TGG + chr1.1:58700290-58700309 MS.gene74841:CDS 50.0%
! GGTGGGGATTTTCTTCAGGT+TGG + chr1.1:58700725-58700744 MS.gene74841:CDS 50.0%
! GTTGGCCTAATACCATGACG+CGG + chr1.1:58700339-58700358 MS.gene74841:CDS 50.0%
! TCCTTGATCAGGTGGTGTTG+GGG - chr1.1:58698767-58698786 None:intergenic 50.0%
! TTTTCACCTCTCCGCTTGTG+TGG + chr1.1:58698218-58698237 MS.gene74841:intron 50.0%
!!! GGGGATTTTCTTCAGGTTGG+TGG + chr1.1:58700728-58700747 MS.gene74841:CDS 50.0%
ACATCTGTCATGGGGCAAGG+TGG - chr1.1:58699217-58699236 None:intergenic 55.0%
ACCACCACCTGCATTGCCTA+TGG + chr1.1:58700259-58700278 MS.gene74841:CDS 55.0%
ACCATAGGCAATGCAGGTGG+TGG - chr1.1:58700263-58700282 None:intergenic 55.0%
CCCAGCTGATCAGGAAGCTA+CGG + chr1.1:58700796-58700815 MS.gene74841:CDS 55.0%
CCGTAGCTTCCTGATCAGCT+GGG - chr1.1:58700799-58700818 None:intergenic 55.0%
CGTAGCTTCCTGATCAGCTG+GGG - chr1.1:58700798-58700817 None:intergenic 55.0%
GCACCATGTCCTTGATCAGG+TGG - chr1.1:58698775-58698794 None:intergenic 55.0%
GCAGTTGCTTCATCATCCGC+AGG - chr1.1:58698979-58698998 None:intergenic 55.0%
GGGTGAACCATAGGCAATGC+AGG - chr1.1:58700269-58700288 None:intergenic 55.0%
TCCCCAACACCACCTGATCA+AGG + chr1.1:58698763-58698782 MS.gene74841:CDS 55.0%
TCCGTAGCTTCCTGATCAGC+TGG - chr1.1:58700800-58700819 None:intergenic 55.0%
TCGACATCTGTCATGGGGCA+AGG - chr1.1:58699220-58699239 None:intergenic 55.0%
!! AATGCAGGTGGTGGTGATGG+TGG - chr1.1:58700254-58700273 None:intergenic 55.0%
!! TGATGGTGGTGGCAGCATGT+TGG - chr1.1:58700240-58700259 None:intergenic 55.0%
TACACGGCCGTGGTTGCTAC+TGG + chr1.1:58697976-58697995 MS.gene74841:intron 60.0%
TGTGCATGCATACACGGCCG+TGG + chr1.1:58697966-58697985 MS.gene74841:intron 60.0%
! GGCAATGCAGGTGGTGGTGA+TGG - chr1.1:58700257-58700276 None:intergenic 60.0%
!! GCTGGGGAAGCACCCATGAA+AGG - chr1.1:58700782-58700801 None:intergenic 60.0%
GGGGGGAGAGGGTGAACCAT+AGG - chr1.1:58700278-58700297 None:intergenic 65.0%
GGGTGCTTCCCCAGCTGATC+AGG + chr1.1:58700787-58700806 MS.gene74841:CDS 65.0%
!! GCAGGTGGTGGTGATGGTGG+TGG - chr1.1:58700251-58700270 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 58697544 58700838 58697544 ID=MS.gene74841
chr1.1 mRNA 58697544 58700838 58697544 ID=MS.gene74841.t1;Parent=MS.gene74841
chr1.1 exon 58697544 58697575 58697544 ID=MS.gene74841.t1.exon1;Parent=MS.gene74841.t1
chr1.1 CDS 58697544 58697575 58697544 ID=cds.MS.gene74841.t1;Parent=MS.gene74841.t1
chr1.1 exon 58698705 58699061 58698705 ID=MS.gene74841.t1.exon2;Parent=MS.gene74841.t1
chr1.1 CDS 58698705 58699061 58698705 ID=cds.MS.gene74841.t1;Parent=MS.gene74841.t1
chr1.1 exon 58699180 58699347 58699180 ID=MS.gene74841.t1.exon3;Parent=MS.gene74841.t1
chr1.1 CDS 58699180 58699347 58699180 ID=cds.MS.gene74841.t1;Parent=MS.gene74841.t1
chr1.1 exon 58700058 58700838 58700058 ID=MS.gene74841.t1.exon4;Parent=MS.gene74841.t1
chr1.1 CDS 58700058 58700838 58700058 ID=cds.MS.gene74841.t1;Parent=MS.gene74841.t1
Gene Sequence

>MS.gene74841

ATGAATCTCCTCCTTCTTCTGGTTATTCCGAGGTATTTATATACTCTTTTTACGCTAATACATGTAAAGATGATGATCAAGATCATGTATTTACAAAATATATATTGAGACAATCATATAATGATTAACTTTTAAAAAATATAAAAAATTTCATACAATTTTTATTTAGTTTATCTATACTATATGTAATATTAATTTTTGATCTAACATTTTTCTCTTTTAATTTTACACTTCAATTAAACTGTTTTGATCATAATTTTATCCTTTTTTATTTTTTGATGAAAATAAAACTAATGATTGATCATTTTAAATTAAATTGAAGCTCGATTTTTTTTTTTAAAGTTAAGCTCAGTTACTTGATGTCATTCATTGTTCAATTAAATAACTAATTTTTAAAATACGTATGTATACACATTGTCGTGTGTGCATGCATACACGGCCGTGGTTGCTACTGGTTCATTTTCATATAAATAAAAATGTAATACAAAGTAGATCTTCTTATTGAACTTTTTAAGCCGAATTTAATTGAAAAGCCATTTTAAATCTTGAATGAAGATGATAAAAAATTGTACCCTTTTTTTTTAAATAATGTCACTTTTTATTATTTACATTATATCAAAATATATTTTCACATTAATTATTATCCAATATAAACGAATCCAAATTCACATCATTTTTCACCTCTCCGCTTGTGTGGTTATTAAAAGTTCTCATTGCAATGCACCATTATTTCTCATTAGGAATATTTTTATTTTCCATTTTAGCACTAATACAACTTAGAACTTGAAACATTTAAACTCGACAAACATATTCCTAATCAGACCTTTAATTCAATCTAACATGTATTTATTATAATAATGTAATAAAATTATTATTCTTTATTTTCTTATCTAATTTTAATTATAATTTATTTTTGTATGTAAAAATATTTTTGTTTTTATAGTTTCATAGGCATATAATGCAATGCAAATAGTTTTACACCAACATGGAATGATTTATTTATTAGATTCAATTAATTTAAATTATGATACATATCATTTAAATTATAATTATTACATGAATGCTTATGAAATAATTAATTATGATTCACTAAAAATATACGTGTGTGTAATTGAATCAAATTCATTAGGCTTCTGCTGATGATTGCCATGTGGCAACAAGAGGAGATGCAAAACTTCAAGCTGAAACTGAAGTTCAACAACCAGTGTTGAATTTCTCATCCCCAACACCACCTGATCAAGGACATGGTGCAGAAGAAGAACAAGACCATGTGCAAAATGATGCAAATGTAGTGGTGAAAAATGCTGAAATTCTAAGGGCAAGGAAAGGGAAGATGCCAATGAACTTTTCTGAAGCAGTGCATGCCAAAGAAGATAGAACAACTGATGATAAAGAAACAAATGTACCAACTGATGATCAAGTTTCAGATACACCTGCGGATGATGAAGCAACTGCCATTGGAGGAATGATGTTGCTGCAATATGATGATGGTGGTGCTAGTGGATCTTGTCCACAAAGGTTTTATAATCACTTTGATAGTGTGAATATATATAGACACGCGTGCACACACACTTACATACATTGTTGCTAATAAATAAAGGTTAACTTTTTTTTGCTCAATTATTTTGCAATTCAGGAAGAGAAAGAGGGACTTCTCTGAATACTATAAACCACCTTGCCCCATGACAGATGTCGAGCAAAAAATAGTGATCCGAGTTGAAAGTGATGAAGACATTTTGGATGATGGTTATTGTTGGCGCAAGTACGGATGCAAAGAGATCAAAGGAAATCCTAACAATCGTAGGTAATTATTTACCTCTTTTATATGTGTTAATGCAAGTATGTGTATATTGATAAATGTTTTTCTTTTTCATTCACTCCTGGCCTTGGTGTATATATAGAGAGGAGGAAAATATGATGCAAGACATCATGCAATAGTACTTGAAAAATCTCTATAAAACCAATGATGATGAACACTGTTGTTGTTTAGAAGTAGAAGCTTACAATTCTCTTTATTTCCACAACTAAGCCAATGACATAATGATCATTGACGGTTTGACGAAAACATGGGAAATCATATATATGCTTTTCCTTGTTTTTGATATTTTTACAATGATAATTTTATTTTATCAAACTTTACAACATCACCGTGTTGAACCAACATCAATCATTGTTAGATTTAGAGATGATTTTCAAGTATTGGGCAAGAATTTATTTCTTCCGATAAGTGATTTATCGTAAAACTTGCCTAGAATTTGATATTAAATTTGTGGTTATACACACATGTTTATGTATATAATTTATCATTTTATTCATCAAACTTTATCATTTTATTTTAAGCGGCTTTCACTATAATTTTAATTATGCATGTGTGTGTGTTTTAAATAGTTACAAAGTTTCTTATCATTATCTAAACATTGTCCATTTACATAAATGTATTAGTATCAATGTATCATGCATCATATATATGAATATGTTTTGATTCCTAATTATTTAATATTGTGTGAATTGTAGGGGGTACTACAGATGCGCAACACACAATTGTGACATTAAAAAACAAGTGGAAAGGGATGCTAAAGATCCAAAATATGTACTTGTTACATATGATGGAAAACATACCCACGGACCTGTCATTGACAAAAAAAGACCAACATACTCGAGAAATATTAATGCATCTGGAGTTCGTAGAAATGCCAACATGCTGCCACCACCATCACCACCACCTGCATTGCCTATGGTTCACCCTCTCCCCCCTTTTCACCAATATGGCTATATACCTTATGGTATGAATTACGGTTGGCCTAATACCATGACGCGGCCTTTATTAAACATGGTGGGTAACACCATGATGCAACCTTACAGACCCATAAGTGAACAAAATTTTCTAGAACATCGTGCTTCAACTCCTATTGATACAAATCAGGTTGGACCAAATATCAACGGGCCAATTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATGACTGCAACTCAAGTTGGACATAATATCAATGGGTCAGTTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATTACCACAACTCAAGTTGGACCTAATATTAATGGGTCAATTTCACTTCCTGTATTTCCGATATCACCACTATTACCACCATTTCACATTGTACCATATGGTGGGGATTTTCTTCAGGTTGGTGGAAATGGTATTGTTGCTGCTCAAGTCTTTCCTTTCATGGGTGCTTCCCCAGCTGATCAGGAAGCTACGGATGTTCCTGACACCGAATGA
Protein sequence

>MS.gene74841.t1

MNLLLLLVIPRGDAKLQAETEVQQPVLNFSSPTPPDQGHGAEEEQDHVQNDANVVVKNAEILRARKGKMPMNFSEAVHAKEDRTTDDKETNVPTDDQVSDTPADDEATAIGGMMLLQYDDGGASGSCPQRKRKRDFSEYYKPPCPMTDVEQKIVIRVESDEDILDDGYCWRKYGCKEIKGNPNNRRGYYRCATHNCDIKKQVERDAKDPKYVLVTYDGKHTHGPVIDKKRPTYSRNINASGVRRNANMLPPPSPPPALPMVHPLPPFHQYGYIPYGMNYGWPNTMTRPLLNMVGNTMMQPYRPISEQNFLEHRASTPIDTNQVGPNINGPIPLPVFPISPPLPPFPMTATQVGHNINGSVPLPVFPISPPLPPFPITTTQVGPNINGSISLPVFPISPLLPPFHIVPYGGDFLQVGGNGIVAAQVFPFMGASPADQEATDVPDTE