Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75161.t1 | KHN11326.1 | 69.5 | 177 | 48 | 3 | 16 | 187 | 1 | 176 | 1.40E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75161.t1 | Q9SY66 | 41.4 | 99 | 49 | 2 | 98 | 187 | 30 | 128 | 6.4e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75161.t1 | A0A0B2RR75 | 69.3 | 176 | 48 | 3 | 17 | 187 | 4 | 178 | 4.0e-57 | 230.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene75161.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75161.t1 | MTR_4g035820 | 58.333 | 168 | 53 | 5 | 21 | 187 | 1 | 152 | 2.28e-55 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75161.t1 | AT1G10240 | 44.048 | 84 | 44 | 1 | 107 | 187 | 45 | 128 | 4.39e-20 | 87.4 |
| MS.gene75161.t1 | AT1G10240 | 44.048 | 84 | 44 | 1 | 107 | 187 | 45 | 128 | 4.39e-20 | 87.4 |
| MS.gene75161.t1 | AT5G28530 | 43.750 | 80 | 44 | 1 | 109 | 187 | 54 | 133 | 2.29e-14 | 70.9 |
| MS.gene75161.t1 | AT5G28530 | 43.750 | 80 | 44 | 1 | 109 | 187 | 54 | 133 | 2.29e-14 | 70.9 |
| MS.gene75161.t1 | AT5G28530 | 43.750 | 80 | 44 | 1 | 109 | 187 | 54 | 133 | 2.32e-14 | 70.9 |
| MS.gene75161.t1 | AT5G28530 | 43.750 | 80 | 44 | 1 | 109 | 187 | 54 | 133 | 2.33e-14 | 70.9 |
| MS.gene75161.t1 | AT5G28530 | 43.750 | 80 | 44 | 1 | 109 | 187 | 54 | 133 | 2.45e-14 | 70.9 |
Find 20 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAGTTACTTTAGAAAA+TGG | 0.196031 | 1.1:-81167918 | MS.gene75161:CDS |
| GGTAGATTCATCAAACAAAA+TGG | 0.329127 | 1.1:-81167633 | MS.gene75161:CDS |
| ATCTCAACATCTTGACTAGA+AGG | 0.417447 | 1.1:+81167795 | None:intergenic |
| TCTAAAGTAACTTCTTCTAC+TGG | 0.417760 | 1.1:+81167924 | None:intergenic |
| AGTCAAGATGTTGAGATGAA+TGG | 0.418700 | 1.1:-81167789 | MS.gene75161:CDS |
| GTGTTAGAAATTGATGAAAT+CGG | 0.428988 | 1.1:-81167843 | MS.gene75161:CDS |
| GTCAAGATGTTGAGATGAAT+GGG | 0.435675 | 1.1:-81167788 | MS.gene75161:CDS |
| CTCAAACTCAAACCACAATC+AGG | 0.443030 | 1.1:-81168171 | MS.gene75161:intron |
| TAGAACCTCTTTGAAAATCA+TGG | 0.444579 | 1.1:-81167571 | MS.gene75161:CDS |
| AGGAAAATTTGACCAACAAA+AGG | 0.448625 | 1.1:+81167723 | None:intergenic |
| TCAAAGAGGTTCTACCTTCA+CGG | 0.449952 | 1.1:+81167580 | None:intergenic |
| CGAATGCTTCTTCCTCACTT+AGG | 0.474844 | 1.1:+81167703 | None:intergenic |
| TGCACGTCAACATGGATTCT+CGG | 0.489863 | 1.1:-81167664 | MS.gene75161:CDS |
| CATGGATTCTCGGTTCGAAA+AGG | 0.524434 | 1.1:-81167654 | MS.gene75161:CDS |
| TATATAAAAGTACCTGATTG+TGG | 0.548243 | 1.1:+81168159 | None:intergenic |
| TTGTGGTTTGAGTTTGAGCT+AGG | 0.554992 | 1.1:+81168176 | None:intergenic |
| AGAAGAAAGTGACGAAGACA+TGG | 0.570974 | 1.1:-81167760 | MS.gene75161:CDS |
| AACAAAATGGAATTGTGAGT+AGG | 0.593662 | 1.1:-81167620 | MS.gene75161:CDS |
| AAGAGGTATGCACGTCAACA+TGG | 0.594612 | 1.1:-81167672 | MS.gene75161:CDS |
| AAAGAAATAGAGAATCAACA+AGG | 0.743349 | 1.1:-81167533 | MS.gene75161:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTAAATATACAAAGTT+TGG | + | chr1.1:81167570-81167589 | None:intergenic | 15.0% |
| !! | TTAATTTCTTCAATGTATTA+TGG | - | chr1.1:81167582-81167601 | MS.gene75161:CDS | 15.0% |
| !!! | AAAAATATTGTTCCTTTTGT+TGG | - | chr1.1:81167968-81167987 | MS.gene75161:CDS | 20.0% |
| ! | AAAGAAATAGAGAATCAACA+AGG | - | chr1.1:81168170-81168189 | MS.gene75161:intron | 25.0% |
| ! | GTGTTAGAAATTGATGAAAT+CGG | - | chr1.1:81167860-81167879 | MS.gene75161:CDS | 25.0% |
| !! | GAAGAAGTTACTTTAGAAAA+TGG | - | chr1.1:81167785-81167804 | MS.gene75161:CDS | 25.0% |
| !!! | AAGCATTCGTTTTTTATAAG+AGG | - | chr1.1:81168014-81168033 | MS.gene75161:intron | 25.0% |
| !!! | ACATTTTGATCTTAACATTG+TGG | - | chr1.1:81167829-81167848 | MS.gene75161:CDS | 25.0% |
| !!! | CTATTTCTTTGTTCTTTTGA+TGG | + | chr1.1:81168160-81168179 | None:intergenic | 25.0% |
| !!! | TATATAAAAGTACCTGATTG+TGG | + | chr1.1:81167547-81167566 | None:intergenic | 25.0% |
| AACAAAATGGAATTGTGAGT+AGG | - | chr1.1:81168083-81168102 | MS.gene75161:intron | 30.0% | |
| AGGAAAATTTGACCAACAAA+AGG | + | chr1.1:81167983-81168002 | None:intergenic | 30.0% | |
| GGTAGATTCATCAAACAAAA+TGG | - | chr1.1:81168070-81168089 | MS.gene75161:intron | 30.0% | |
| ! | TAGAACCTCTTTGAAAATCA+TGG | - | chr1.1:81168132-81168151 | MS.gene75161:intron | 30.0% |
| ! | TCTAAAGTAACTTCTTCTAC+TGG | + | chr1.1:81167782-81167801 | None:intergenic | 30.0% |
| AGTCAAGATGTTGAGATGAA+TGG | - | chr1.1:81167914-81167933 | MS.gene75161:CDS | 35.0% | |
| ATCTCAACATCTTGACTAGA+AGG | + | chr1.1:81167911-81167930 | None:intergenic | 35.0% | |
| GTCAAGATGTTGAGATGAAT+GGG | - | chr1.1:81167915-81167934 | MS.gene75161:CDS | 35.0% | |
| ! | TGGTCAAATTTTCCTAAGTG+AGG | - | chr1.1:81167988-81168007 | MS.gene75161:CDS | 35.0% |
| ! | TGGTTCCATGATTTTCAAAG+AGG | + | chr1.1:81168140-81168159 | None:intergenic | 35.0% |
| AGAAGAAAGTGACGAAGACA+TGG | - | chr1.1:81167943-81167962 | MS.gene75161:CDS | 40.0% | |
| CTCAAACTCAAACCACAATC+AGG | - | chr1.1:81167532-81167551 | MS.gene75161:CDS | 40.0% | |
| TCAAAGAGGTTCTACCTTCA+CGG | + | chr1.1:81168126-81168145 | None:intergenic | 40.0% | |
| ! | GATTTCTTTTGTCACCGTGA+AGG | - | chr1.1:81168109-81168128 | MS.gene75161:intron | 40.0% |
| ! | TTGTGGTTTGAGTTTGAGCT+AGG | + | chr1.1:81167530-81167549 | None:intergenic | 40.0% |
| CGAATGCTTCTTCCTCACTT+AGG | + | chr1.1:81168003-81168022 | None:intergenic | 45.0% | |
| TGCACGTCAACATGGATTCT+CGG | - | chr1.1:81168039-81168058 | MS.gene75161:intron | 45.0% | |
| ! | AAGAGGTATGCACGTCAACA+TGG | - | chr1.1:81168031-81168050 | MS.gene75161:intron | 45.0% |
| ! | CATGGATTCTCGGTTCGAAA+AGG | - | chr1.1:81168049-81168068 | MS.gene75161:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 81167509 | 81168216 | 81167509 | ID=MS.gene75161 |
| chr1.1 | mRNA | 81167509 | 81168216 | 81167509 | ID=MS.gene75161.t1;Parent=MS.gene75161 |
| chr1.1 | exon | 81168172 | 81168216 | 81168172 | ID=MS.gene75161.t1.exon1;Parent=MS.gene75161.t1 |
| chr1.1 | CDS | 81168172 | 81168216 | 81168172 | ID=cds.MS.gene75161.t1;Parent=MS.gene75161.t1 |
| chr1.1 | exon | 81167509 | 81168027 | 81167509 | ID=MS.gene75161.t1.exon2;Parent=MS.gene75161.t1 |
| chr1.1 | CDS | 81167509 | 81168027 | 81167509 | ID=cds.MS.gene75161.t1;Parent=MS.gene75161.t1 |
| Gene Sequence |
| Protein sequence |
