AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75192

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75192.t1 MTR_1g109320 99.160 357 3 0 1 357 1 357 0.0 738
MS.gene75192.t1 MTR_5g090040 66.667 105 18 2 241 345 63 150 8.60e-43 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75192.t1 AT1G68200 49.020 306 109 8 62 352 29 302 5.73e-85 260
MS.gene75192.t1 AT1G68200 49.020 306 109 8 62 352 29 302 5.73e-85 260
MS.gene75192.t1 AT1G68200 48.693 306 109 8 62 352 29 301 2.03e-83 256
MS.gene75192.t1 AT1G68200 48.693 306 109 8 62 352 29 301 2.03e-83 256
MS.gene75192.t1 AT1G66810 61.644 146 47 1 200 345 168 304 2.34e-55 184
MS.gene75192.t1 AT1G66810 61.644 146 47 1 200 345 168 304 2.34e-55 184
MS.gene75192.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.63e-22 92.8
MS.gene75192.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.63e-22 92.8

Find 103 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCAGGATGGTCCTTGC+TGG 0.159266 1.3:-74341747 MS.gene75192:CDS
CTGCAGAGCTTCAACTTCTT+TGG 0.179049 1.3:+74344270 None:intergenic
TCTTTCCAGCTTCATTGATC+TGG 0.216499 1.3:+74341644 None:intergenic
GCCGCCGTTGCTGCTGTTTC+CGG 0.266911 1.3:+74344411 None:intergenic
GCCGATGCCGTAACCGCTTT+TGG 0.267264 1.3:+74344522 None:intergenic
CAACTTCTTTGGAACTTTCC+AGG 0.279329 1.3:+74344281 None:intergenic
TAGCGTGGGTGGCGGATCAC+TGG 0.295264 1.3:+74341780 None:intergenic
GATCTAACAGAAATGCTCTT+TGG 0.302932 1.3:+74343941 None:intergenic
GCGGGAGCGAGTAGCGCTTT+TGG 0.327959 1.3:+74343856 None:intergenic
GTCAATTCGCTCATGGCATT+GGG 0.349776 1.3:-74341812 MS.gene75192:CDS
GGTTTCCGTGGCTTGAATCT+TGG 0.355570 1.3:-74344106 MS.gene75192:CDS
CTTTCCAGCTTCATTGATCT+GGG 0.356760 1.3:+74341645 None:intergenic
CATGACGGAAATGGCATCTA+TGG 0.367595 1.3:+74341706 None:intergenic
CTTTATCCTTACTCTGACCT+TGG 0.377357 1.3:-74344379 MS.gene75192:CDS
GAAACTGGCACATGCCCTTA+TGG 0.383739 1.3:-74341843 MS.gene75192:CDS
TAATAATAACAAGGAGTTGC+AGG 0.387088 1.3:-74344038 MS.gene75192:CDS
CCGTCATCCAAAAGCGGTTA+CGG 0.390482 1.3:-74344529 MS.gene75192:CDS
GATACAGAAGCATCAGGATA+TGG 0.396830 1.3:-74344335 MS.gene75192:CDS
CTGTTTCCGGCGAGCGATGA+TGG 0.396990 1.3:+74344424 None:intergenic
TGTAACAAATGGCAGGAAAC+TGG 0.399409 1.3:-74341858 MS.gene75192:CDS
TCTGCAGGATGGTCCTTGCT+GGG 0.402584 1.3:-74341746 MS.gene75192:CDS
CTTCTGCAACTCTTTGTTCA+CGG 0.410248 1.3:+74344225 None:intergenic
CAGTAAGTGCATGACGGAAA+TGG 0.414882 1.3:+74341697 None:intergenic
CCTTCTCGTTCAAGCTTCGC+TGG 0.421155 1.3:-74344194 MS.gene75192:CDS
TATGTTTCTCAGTCGTCTCC+TGG 0.438714 1.3:-74344299 MS.gene75192:CDS
CAAACGGAGTTGATTGAGTC+TGG 0.444450 1.3:+74344140 None:intergenic
TGTCAATTCGCTCATGGCAT+TGG 0.444853 1.3:-74341813 MS.gene75192:CDS
CTTGAAGCTTGCTCCGCCTC+CGG 0.468503 1.3:-74343909 MS.gene75192:CDS
AATTGACAGTGGTCTCCATA+AGG 0.472418 1.3:+74341828 None:intergenic
CGTGGCTTGAATCTTGGAGA+TGG 0.475661 1.3:-74344100 MS.gene75192:CDS
GAAAGGAAAACTGTGCTGAT+TGG 0.476246 1.3:-74344078 MS.gene75192:CDS
GGCGTTCGACTGCATGTGAC+CGG 0.476954 1.3:+74344489 None:intergenic
AGCATTTCTGTTAGATCCAA+TGG 0.486635 1.3:-74343935 MS.gene75192:CDS
TCCAAAAGCGGTTACGGCAT+CGG 0.490846 1.3:-74344523 MS.gene75192:CDS
CGTCGGACTCTCATCAGATT+CGG 0.492348 1.3:+74344009 None:intergenic
GAAGCTCTGCAGCAGGAAAA+CGG 0.500754 1.3:-74344259 MS.gene75192:CDS
AAACCTTGCAACACATCAAA+CGG 0.507246 1.3:+74344124 None:intergenic
TGAGAATAACGGTGTTGAAA+CGG 0.507485 1.3:-74343978 MS.gene75192:CDS
AGCGTGGGTGGCGGATCACT+GGG 0.510898 1.3:+74341781 None:intergenic
AAATGGCATCTATGGCCATA+CGG 0.511220 1.3:+74341714 None:intergenic
GCTGGGGTTGTGTGCCCGTA+TGG 0.512898 1.3:-74341729 MS.gene75192:CDS
ATGCGTGATACAGAAGCATC+AGG 0.518868 1.3:-74344341 MS.gene75192:CDS
TCGCTGGAGAACCGATTCAA+TGG 0.519751 1.3:-74344178 MS.gene75192:CDS
CTCGCCGGAAACAGCAGCAA+CGG 0.524709 1.3:-74344415 MS.gene75192:CDS
GGAAACAGCAGCAACGGCGG+CGG 0.524773 1.3:-74344409 MS.gene75192:CDS
GCCGGAAACAGCAGCAACGG+CGG 0.525054 1.3:-74344412 MS.gene75192:CDS
GCTACAAAACTGAGGTCTGC+AGG 0.527724 1.3:-74341761 MS.gene75192:CDS
TTAGTTACGACGGCCGGAGG+CGG 0.529646 1.3:+74343896 None:intergenic
AGAAGTTGAAGCTCTGCAGC+AGG 0.529958 1.3:-74344266 MS.gene75192:CDS
ATTGACAGTGGTCTCCATAA+GGG 0.530052 1.3:+74341829 None:intergenic
TAAGCCCAGATCAATGAAGC+TGG 0.530814 1.3:-74341649 MS.gene75192:CDS
AGCAAGCTTCAAGTAGCCAT+TGG 0.531440 1.3:+74343919 None:intergenic
AGACTGAGCTCTGTAACAAA+TGG 0.531908 1.3:-74341869 MS.gene75192:CDS
AATGGCATCTATGGCCATAC+GGG 0.531942 1.3:+74341715 None:intergenic
AGGATCCGCCACCATTGAAT+CGG 0.534519 1.3:+74344167 None:intergenic
GCTCAGTCTTGAACATCCCC+TGG 0.541107 1.3:+74341883 None:intergenic
TCTGTTCCAAGGTCAGAGTA+AGG 0.546191 1.3:+74344373 None:intergenic
GATCTTCCATCATCGCTCGC+CGG 0.559218 1.3:-74344430 MS.gene75192:CDS
ACTCCGTTTGATGTGTTGCA+AGG 0.560653 1.3:-74344127 MS.gene75192:CDS
TGAGCTCTGTAACAAATGGC+AGG 0.566245 1.3:-74341865 MS.gene75192:CDS
AGCATTGTTGTTAGTTACGA+CGG 0.567553 1.3:+74343886 None:intergenic
CATCTCCAAGATTCAAGCCA+CGG 0.567612 1.3:+74344101 None:intergenic
TGTGCGAGGAGGGCAGAAAG+AGG 0.570699 1.3:-74341934 MS.gene75192:CDS
GACCACTGTCAATTCGCTCA+TGG 0.571725 1.3:-74341819 MS.gene75192:CDS
CTGCAGGATGGTCCTTGCTG+GGG 0.572424 1.3:-74341745 MS.gene75192:CDS
AGAGGAAGAGCCTCTTGAGA+TGG 0.573431 1.3:-74341916 MS.gene75192:CDS
AAAAGGTATTTGTGCGAGGA+GGG 0.574881 1.3:-74341944 MS.gene75192:CDS
GAGAACCGATTCAATGGTGG+CGG 0.578241 1.3:-74344172 MS.gene75192:CDS
CCAGCGAAGCTTGAACGAGA+AGG 0.578885 1.3:+74344194 None:intergenic
TACAACTGTATCAGATTGAG+TGG 0.578967 1.3:+74343826 None:intergenic
TGAGATGGTTGTGTACAACC+AGG 0.582022 1.3:-74341901 MS.gene75192:CDS
CTTGAATCTTGGAGATGGAA+AGG 0.582921 1.3:-74344095 MS.gene75192:CDS
CAGATTGAGTGGAGGAAGCG+CGG 0.588323 1.3:+74343837 None:intergenic
CGGAGTTGATTGAGTCTGGA+CGG 0.589982 1.3:+74344144 None:intergenic
GCAACTCTTTGTTCACGGCA+CGG 0.595404 1.3:+74344230 None:intergenic
AGATTGAGTGGAGGAAGCGC+GGG 0.600576 1.3:+74343838 None:intergenic
CAAAAGGTATTTGTGCGAGG+AGG 0.603500 1.3:-74341945 MS.gene75192:CDS
TTGTTGTTAGTTACGACGGC+CGG 0.605294 1.3:+74343890 None:intergenic
GATGTGTTGCAAGGTTTCCG+TGG 0.607851 1.3:-74344118 MS.gene75192:CDS
GGTCACATGCAGTCGAACGC+CGG 0.607925 1.3:-74344487 MS.gene75192:CDS
CTTGTTCAGTAAGTGCATGA+CGG 0.608476 1.3:+74341691 None:intergenic
CAAAACTGAGGTCTGCAGGA+TGG 0.616724 1.3:-74341757 MS.gene75192:CDS
ACGAGTGTGATTGAGAATAA+CGG 0.622937 1.3:-74343989 MS.gene75192:CDS
ATGAGTCGTACTCTGTTCCA+AGG 0.623681 1.3:+74344362 None:intergenic
ACGGGCACACAACCCCAGCA+AGG 0.626804 1.3:+74341733 None:intergenic
CTGGAGAACCGATTCAATGG+TGG 0.634958 1.3:-74344175 MS.gene75192:CDS
TGTTCTCCGTCATCCAAAAG+CGG 0.637659 1.3:-74344535 MS.gene75192:CDS
GAGATGGTTGTGTACAACCA+GGG 0.638365 1.3:-74341900 MS.gene75192:CDS
TTGTTAGTTACGACGGCCGG+AGG 0.641381 1.3:+74343893 None:intergenic
CAGCAAAAGGTATTTGTGCG+AGG 0.645044 1.3:-74341948 MS.gene75192:CDS
GGCATCGGCGTCAACAACGC+CGG 0.645707 1.3:-74344508 MS.gene75192:CDS
TCAATTCGCTCATGGCATTG+GGG 0.646224 1.3:-74341811 MS.gene75192:CDS
TTGTACACAACCATCTCAAG+AGG 0.647753 1.3:+74341906 None:intergenic
ACCGATCAAAATGCATAACA+AGG 0.654571 1.3:-74344563 None:intergenic
AACTGTATCAGATTGAGTGG+AGG 0.659126 1.3:+74343829 None:intergenic
TAACATCAATAATAATAACA+AGG 0.675567 1.3:-74344047 MS.gene75192:CDS
TGCCATGAGCGAATTGACAG+TGG 0.682669 1.3:+74341817 None:intergenic
TTATTCTCAATCACACTCGT+CGG 0.688787 1.3:+74343992 None:intergenic
AGTTGATTGAGTCTGGACGG+AGG 0.689033 1.3:+74344147 None:intergenic
ATAACGGTGTTGAAACGGAG+AGG 0.717587 1.3:-74343973 MS.gene75192:CDS
CCACCCACGCTACAAAACTG+AGG 0.721664 1.3:-74341769 MS.gene75192:CDS
AGATGGTTGTGTACAACCAG+GGG 0.741581 1.3:-74341899 MS.gene75192:CDS
GTACAACGAAGCAAACACGC+CGG 0.782658 1.3:+74344468 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTATGTTGAAAATA+AGG - chr1.3:74343914-74343933 MS.gene75192:CDS 10.0%
!! AGCATATATATTAATAATAG+AGG + chr1.3:74343891-74343910 None:intergenic 15.0%
!! ATTATAATGTAAAAACTGTA+TGG + chr1.3:74343386-74343405 None:intergenic 15.0%
!! TAACAAAACAAATAACTAAA+TGG + chr1.3:74343796-74343815 None:intergenic 15.0%
!! TAACATCAATAATAATAACA+AGG - chr1.3:74342147-74342166 MS.gene75192:intron 15.0%
!!! CAGTTTTTACATTATAATTA+AGG - chr1.3:74343388-74343407 MS.gene75192:intron 15.0%
!!! TTTTTACATTATAATTAAGG+TGG - chr1.3:74343391-74343410 MS.gene75192:intron 15.0%
!!! AATTGTGTTCAATTTTGAAA+TGG - chr1.3:74343972-74343991 MS.gene75192:CDS 20.0%
!!! CGATATTTTAAACTAATAGA+TGG - chr1.3:74343125-74343144 MS.gene75192:intron 20.0%
!!! GCATCAAAATTTAACTTAAT+TGG + chr1.3:74343361-74343380 None:intergenic 20.0%
!!! TAGTTTTGATTGTTGATATT+CGG - chr1.3:74344184-74344203 MS.gene75192:CDS 20.0%
!!! TGGTTTTTCTAAGTTTTAAA+GGG - chr1.3:74343631-74343650 MS.gene75192:intron 20.0%
!!! TTGGTTTTTCTAAGTTTTAA+AGG - chr1.3:74343630-74343649 MS.gene75192:intron 20.0%
!!! TTTAAAACTTAGAAAAACCA+AGG + chr1.3:74343631-74343650 None:intergenic 20.0%
! TCATACAATGTCTACATTAT+AGG - chr1.3:74343492-74343511 MS.gene75192:intron 25.0%
!!! ACATTATAGGAGTATTTAAG+AGG - chr1.3:74343505-74343524 MS.gene75192:intron 25.0%
!!! ATGAGATAAAGATTTTAGAG+AGG - chr1.3:74343153-74343172 MS.gene75192:intron 25.0%
!!! GGTTTTTCTAAGTTTTAAAG+GGG - chr1.3:74343632-74343651 MS.gene75192:intron 25.0%
!!! TAAAGATTTTAGAGAGGAAA+CGG - chr1.3:74343159-74343178 MS.gene75192:intron 25.0%
!!! TAAGTTTTAAAGGGGATAAA+TGG - chr1.3:74343640-74343659 MS.gene75192:intron 25.0%
AAACATTAGTATGACTGTCA+AGG + chr1.3:74344027-74344046 None:intergenic 30.0%
AACCAACAACTAGTATAAAC+TGG + chr1.3:74342772-74342791 None:intergenic 30.0%
ACATTTATGATAGCCATGTA+TGG - chr1.3:74343567-74343586 MS.gene75192:intron 30.0%
ACTAACAAGCAAGTCAAATT+TGG + chr1.3:74342618-74342637 None:intergenic 30.0%
CAGGTTAAAATGTCACAAAA+AGG + chr1.3:74342863-74342882 None:intergenic 30.0%
CTGTATATGGTTTCAAATTG+CGG - chr1.3:74342902-74342921 MS.gene75192:intron 30.0%
GAAAAAACAACCAAACTACA+AGG + chr1.3:74342712-74342731 None:intergenic 30.0%
GTAATGTCTTGAATCTTCAT+TGG - chr1.3:74343832-74343851 MS.gene75192:CDS 30.0%
TAATAATAACAAGGAGTTGC+AGG - chr1.3:74342156-74342175 MS.gene75192:intron 30.0%
TACTTCGAGATAAAGATGAA+TGG - chr1.3:74343941-74343960 MS.gene75192:CDS 30.0%
TATATAGAACTAGCAAGACT+AGG - chr1.3:74343457-74343476 MS.gene75192:intron 30.0%
TTACATTATTATGAGCGTCA+AGG + chr1.3:74344125-74344144 None:intergenic 30.0%
! AAGCAAGGTTTTACTTCATT+TGG + chr1.3:74342658-74342677 None:intergenic 30.0%
! AAGGTTTTACTTCATTTGGA+AGG + chr1.3:74342654-74342673 None:intergenic 30.0%
! TGTCACTCAATTTCTTTTCA+TGG - chr1.3:74343709-74343728 MS.gene75192:intron 30.0%
!! TTTTAAAGGGGATAAATGGT+TGG - chr1.3:74343644-74343663 MS.gene75192:intron 30.0%
!!! ACAACCATGGTTTTAAAATG+CGG + chr1.3:74343319-74343338 None:intergenic 30.0%
AAACCATATACAGAGCACAT+TGG + chr1.3:74342895-74342914 None:intergenic 35.0%
AAACCTTGCAACACATCAAA+CGG + chr1.3:74342073-74342092 None:intergenic 35.0%
ACGAGTGTGATTGAGAATAA+CGG - chr1.3:74342205-74342224 MS.gene75192:intron 35.0%
AGCATTTCTGTTAGATCCAA+TGG - chr1.3:74342259-74342278 MS.gene75192:intron 35.0%
ATGGCAAACTAGAGCTAATT+TGG + chr1.3:74342810-74342829 None:intergenic 35.0%
CAATAGCTGCAATATTGCAA+AGG - chr1.3:74343275-74343294 MS.gene75192:intron 35.0%
CTCCAGTTTATACTAGTTGT+TGG - chr1.3:74342767-74342786 MS.gene75192:intron 35.0%
CTGTAACAAACCTTGTAGTT+TGG - chr1.3:74342699-74342718 MS.gene75192:intron 35.0%
GATCTAACAGAAATGCTCTT+TGG + chr1.3:74342256-74342275 None:intergenic 35.0%
TACAACTGTATCAGATTGAG+TGG + chr1.3:74342371-74342390 None:intergenic 35.0%
TACAAGTAACAAGTGATGCA+GGG - chr1.3:74343675-74343694 MS.gene75192:intron 35.0%
TGTTACTATACTGCAGCAAA+AGG - chr1.3:74344233-74344252 MS.gene75192:CDS 35.0%
TTACAAGTAACAAGTGATGC+AGG - chr1.3:74343674-74343693 MS.gene75192:intron 35.0%
TTAGTATGACTGTCAAGGAA+TGG + chr1.3:74344022-74344041 None:intergenic 35.0%
TTATGATCACACCACAATTG+CGG + chr1.3:74342974-74342993 None:intergenic 35.0%
TTATTCTCAATCACACTCGT+CGG + chr1.3:74342205-74342224 None:intergenic 35.0%
! AGCATTGTTGTTAGTTACGA+CGG + chr1.3:74342311-74342330 None:intergenic 35.0%
! GAGGTTTGAAATCAATACCT+TGG - chr1.3:74343611-74343630 MS.gene75192:intron 35.0%
! GTCCACTCAGTGTTATTAAT+CGG + chr1.3:74344158-74344177 None:intergenic 35.0%
! TGAGAATAACGGTGTTGAAA+CGG - chr1.3:74342216-74342235 MS.gene75192:intron 35.0%
!! AAAATTAAGTTGACGCCACA+AGG - chr1.3:74343236-74343255 MS.gene75192:intron 35.0%
AAACCATGGTTGTTTGTGCT+TGG - chr1.3:74343326-74343345 MS.gene75192:intron 40.0%
AAATGGCATCTATGGCCATA+CGG + chr1.3:74344483-74344502 None:intergenic 40.0%
AACTGTATCAGATTGAGTGG+AGG + chr1.3:74342368-74342387 None:intergenic 40.0%
AATTGACAGTGGTCTCCATA+AGG + chr1.3:74344369-74344388 None:intergenic 40.0%
ACAAGTAACAAGTGATGCAG+GGG - chr1.3:74343676-74343695 MS.gene75192:intron 40.0%
AGACTGAGCTCTGTAACAAA+TGG - chr1.3:74344325-74344344 MS.gene75192:CDS 40.0%
ATGGAAATTTGCAGTGCTGT+TGG - chr1.3:74343586-74343605 MS.gene75192:intron 40.0%
ATTGACAGTGGTCTCCATAA+GGG + chr1.3:74344368-74344387 None:intergenic 40.0%
CTTCTGCAACTCTTTGTTCA+CGG + chr1.3:74341972-74341991 None:intergenic 40.0%
CTTGAATCTTGGAGATGGAA+AGG - chr1.3:74342099-74342118 MS.gene75192:intron 40.0%
CTTGTTCAGTAAGTGCATGA+CGG + chr1.3:74344506-74344525 None:intergenic 40.0%
CTTTATCCTTACTCTGACCT+TGG - chr1.3:74341815-74341834 MS.gene75192:CDS 40.0%
CTTTCCAGCTTCATTGATCT+GGG + chr1.3:74344552-74344571 None:intergenic 40.0%
GCACTGCAAATTTCCATACA+TGG + chr1.3:74343583-74343602 None:intergenic 40.0%
TCTTTCCAGCTTCATTGATC+TGG + chr1.3:74344553-74344572 None:intergenic 40.0%
TGTAACAAATGGCAGGAAAC+TGG - chr1.3:74344336-74344355 MS.gene75192:CDS 40.0%
TTATTATGAGCGTCAAGGCA+TGG + chr1.3:74344120-74344139 None:intergenic 40.0%
TTGTACACAACCATCTCAAG+AGG + chr1.3:74344291-74344310 None:intergenic 40.0%
! CACCGATTAATAACACTGAG+TGG - chr1.3:74344153-74344172 MS.gene75192:CDS 40.0%
! GAAAGGAAAACTGTGCTGAT+TGG - chr1.3:74342116-74342135 MS.gene75192:intron 40.0%
! TTGTTACTCACTGCTGACTA+TGG - chr1.3:74343810-74343829 MS.gene75192:intron 40.0%
! TTGTTGGTTAGTAGTGTTGC+AGG - chr1.3:74342783-74342802 MS.gene75192:intron 40.0%
!! AAAAGGTATTTGTGCGAGGA+GGG - chr1.3:74344250-74344269 MS.gene75192:CDS 40.0%
!! ATTAGCTCTAGTTTGCCATG+AGG - chr1.3:74342811-74342830 MS.gene75192:intron 40.0%
!! CAACTTCTTTGGAACTTTCC+AGG + chr1.3:74341916-74341935 None:intergenic 40.0%
!! CAAGGTTTGTTACAGCTAAC+AGG + chr1.3:74342694-74342713 None:intergenic 40.0%
!! GAATCTTCATTGGTTAGCCT+TGG - chr1.3:74343842-74343861 MS.gene75192:CDS 40.0%
!! GATACAGAAGCATCAGGATA+TGG - chr1.3:74341859-74341878 MS.gene75192:CDS 40.0%
!!! ATGGTTTTATCCACTGCTGT+TGG - chr1.3:74343728-74343747 MS.gene75192:intron 40.0%
AAGGGGATAAATGGTTGGAC+TGG - chr1.3:74343649-74343668 MS.gene75192:intron 45.0%
AATGGCATCTATGGCCATAC+GGG + chr1.3:74344482-74344501 None:intergenic 45.0%
ACACCAAGCACAAACAACCA+TGG + chr1.3:74343332-74343351 None:intergenic 45.0%
ACTCCGTTTGATGTGTTGCA+AGG - chr1.3:74342067-74342086 MS.gene75192:intron 45.0%
AGATGGTTGTGTACAACCAG+GGG - chr1.3:74344295-74344314 MS.gene75192:CDS 45.0%
AGCAAGCTTCAAGTAGCCAT+TGG + chr1.3:74342278-74342297 None:intergenic 45.0%
AGTGCCGTAATGAGGGAATT+GGG - chr1.3:74343016-74343035 MS.gene75192:intron 45.0%
ATGACTGTCAAGGAATGGCA+AGG + chr1.3:74344017-74344036 None:intergenic 45.0%
ATGAGTCGTACTCTGTTCCA+AGG + chr1.3:74341835-74341854 None:intergenic 45.0%
CATCTCCAAGATTCAAGCCA+CGG + chr1.3:74342096-74342115 None:intergenic 45.0%
CTGCAGAGCTTCAACTTCTT+TGG + chr1.3:74341927-74341946 None:intergenic 45.0%
CTTGGACACATCTTGTGCTT+GGG - chr1.3:74343860-74343879 MS.gene75192:CDS 45.0%
GAGATGGTTGTGTACAACCA+GGG - chr1.3:74344294-74344313 MS.gene75192:CDS 45.0%
GATGTGTATTGTCGTCCTTG+TGG + chr1.3:74343254-74343273 None:intergenic 45.0%
GGTAATGACACAGACAAGCA+AGG + chr1.3:74342673-74342692 None:intergenic 45.0%
GTCAATTCGCTCATGGCATT+GGG - chr1.3:74344382-74344401 MS.gene75192:CDS 45.0%
GTGCCAATGTGCTCTGTATA+TGG - chr1.3:74342889-74342908 MS.gene75192:intron 45.0%
TAAGCCCAGATCAATGAAGC+TGG - chr1.3:74344545-74344564 MS.gene75192:CDS 45.0%
TATGTTTCTCAGTCGTCTCC+TGG - chr1.3:74341895-74341914 MS.gene75192:CDS 45.0%
TCAATTCGCTCATGGCATTG+GGG - chr1.3:74344383-74344402 MS.gene75192:CDS 45.0%
TCTGTTCCAAGGTCAGAGTA+AGG + chr1.3:74341824-74341843 None:intergenic 45.0%
TGAGATGGTTGTGTACAACC+AGG - chr1.3:74344293-74344312 MS.gene75192:CDS 45.0%
TGAGCTCTGTAACAAATGGC+AGG - chr1.3:74344329-74344348 MS.gene75192:CDS 45.0%
TGCACCCAATTCCCTCATTA+CGG + chr1.3:74343023-74343042 None:intergenic 45.0%
TGTCAATTCGCTCATGGCAT+TGG - chr1.3:74344381-74344400 MS.gene75192:CDS 45.0%
TGTTCTCCGTCATCCAAAAG+CGG - chr1.3:74341659-74341678 MS.gene75192:CDS 45.0%
! AGCTCATCTGTTTTGCCTCA+TGG + chr1.3:74342829-74342848 None:intergenic 45.0%
! ATAACGGTGTTGAAACGGAG+AGG - chr1.3:74342221-74342240 MS.gene75192:intron 45.0%
! ATTTGCAGTGCTGTTGGTAG+AGG - chr1.3:74343592-74343611 MS.gene75192:intron 45.0%
! CAAACGGAGTTGATTGAGTC+TGG + chr1.3:74342057-74342076 None:intergenic 45.0%
! CAGTAAGTGCATGACGGAAA+TGG + chr1.3:74344500-74344519 None:intergenic 45.0%
! CATGACGGAAATGGCATCTA+TGG + chr1.3:74344491-74344510 None:intergenic 45.0%
! TTGTTGTTAGTTACGACGGC+CGG + chr1.3:74342307-74342326 None:intergenic 45.0%
!! AGACCTCAGTTTTGTAGCGT+GGG + chr1.3:74344431-74344450 None:intergenic 45.0%
!! ATGCGTGATACAGAAGCATC+AGG - chr1.3:74341853-74341872 MS.gene75192:CDS 45.0%
!! CAAAAGGTATTTGTGCGAGG+AGG - chr1.3:74344249-74344268 MS.gene75192:CDS 45.0%
!! CAGCAAAAGGTATTTGTGCG+AGG - chr1.3:74344246-74344265 MS.gene75192:CDS 45.0%
!!! ATTTACTATTATTTATTTTA+TGG + chr1.3:74343197-74343216 None:intergenic 5.0%
AGAAGTTGAAGCTCTGCAGC+AGG - chr1.3:74341928-74341947 MS.gene75192:CDS 50.0%
AGAGGAAGAGCCTCTTGAGA+TGG - chr1.3:74344278-74344297 MS.gene75192:CDS 50.0%
AGCACATTGGCACAGAACAC+AGG + chr1.3:74342882-74342901 None:intergenic 50.0%
AGGATCCGCCACCATTGAAT+CGG + chr1.3:74342030-74342049 None:intergenic 50.0%
AGTTGATTGAGTCTGGACGG+AGG + chr1.3:74342050-74342069 None:intergenic 50.0%
ATCGCATCAAGCCGCAATTG+TGG - chr1.3:74342960-74342979 MS.gene75192:intron 50.0%
CAAAACTGAGGTCTGCAGGA+TGG - chr1.3:74344437-74344456 MS.gene75192:CDS 50.0%
CAGTGCCGTAATGAGGGAAT+TGG - chr1.3:74343015-74343034 MS.gene75192:intron 50.0%
CCAAGCACAAGATGTGTCCA+AGG + chr1.3:74343862-74343881 None:intergenic 50.0%
CCGTCATCCAAAAGCGGTTA+CGG - chr1.3:74341665-74341684 MS.gene75192:CDS 50.0%
CCTTGGACACATCTTGTGCT+TGG - chr1.3:74343859-74343878 MS.gene75192:CDS 50.0%
CGTCGGACTCTCATCAGATT+CGG + chr1.3:74342188-74342207 None:intergenic 50.0%
CGTGGCTTGAATCTTGGAGA+TGG - chr1.3:74342094-74342113 MS.gene75192:intron 50.0%
GAAACTGGCACATGCCCTTA+TGG - chr1.3:74344351-74344370 MS.gene75192:CDS 50.0%
GAAAGCTACACCAACAGCAG+TGG + chr1.3:74343741-74343760 None:intergenic 50.0%
GAAGCTCTGCAGCAGGAAAA+CGG - chr1.3:74341935-74341954 MS.gene75192:CDS 50.0%
GACCACTGTCAATTCGCTCA+TGG - chr1.3:74344375-74344394 MS.gene75192:CDS 50.0%
GATGTGTTGCAAGGTTTCCG+TGG - chr1.3:74342076-74342095 MS.gene75192:intron 50.0%
GCAACTCTTTGTTCACGGCA+CGG + chr1.3:74341967-74341986 None:intergenic 50.0%
GCGATTGCAGACAATACCGT+GGG + chr1.3:74342945-74342964 None:intergenic 50.0%
GCTACAAAACTGAGGTCTGC+AGG - chr1.3:74344433-74344452 MS.gene75192:CDS 50.0%
GGTTTCCGTGGCTTGAATCT+TGG - chr1.3:74342088-74342107 MS.gene75192:intron 50.0%
GTACAACGAAGCAAACACGC+CGG + chr1.3:74341729-74341748 None:intergenic 50.0%
TCCAAAAGCGGTTACGGCAT+CGG - chr1.3:74341671-74341690 MS.gene75192:CDS 50.0%
TGCCATGAGCGAATTGACAG+TGG + chr1.3:74344380-74344399 None:intergenic 50.0%
TGCGATTGCAGACAATACCG+TGG + chr1.3:74342946-74342965 None:intergenic 50.0%
TGTCAAGGAATGGCAAGGCA+GGG + chr1.3:74344012-74344031 None:intergenic 50.0%
! CGGAGTTGATTGAGTCTGGA+CGG + chr1.3:74342053-74342072 None:intergenic 50.0%
! CTGGAGAACCGATTCAATGG+TGG - chr1.3:74342019-74342038 MS.gene75192:intron 50.0%
! GAGAACCGATTCAATGGTGG+CGG - chr1.3:74342022-74342041 MS.gene75192:intron 50.0%
! TCGCTGGAGAACCGATTCAA+TGG - chr1.3:74342016-74342035 MS.gene75192:intron 50.0%
!! CAGACCTCAGTTTTGTAGCG+TGG + chr1.3:74344432-74344451 None:intergenic 50.0%
!! CCGTAACCGCTTTTGGATGA+CGG + chr1.3:74341668-74341687 None:intergenic 50.0%
!!! GCGGCCGCATTTTAAAACCA+TGG - chr1.3:74343312-74343331 MS.gene75192:intron 50.0%
AAAGGCAGCGACTGCAACAG+CGG - chr1.3:74343293-74343312 MS.gene75192:intron 55.0%
ACGTCGCAGTGCCGTAATGA+GGG - chr1.3:74343009-74343028 MS.gene75192:intron 55.0%
CAGATTGAGTGGAGGAAGCG+CGG + chr1.3:74342360-74342379 None:intergenic 55.0%
CCACCCACGCTACAAAACTG+AGG - chr1.3:74344425-74344444 MS.gene75192:CDS 55.0%
CCAGCGAAGCTTGAACGAGA+AGG + chr1.3:74342003-74342022 None:intergenic 55.0%
CCTTCTCGTTCAAGCTTCGC+TGG - chr1.3:74342000-74342019 MS.gene75192:intron 55.0%
CTGTCAAGGAATGGCAAGGC+AGG + chr1.3:74344013-74344032 None:intergenic 55.0%
GATCTTCCATCATCGCTCGC+CGG - chr1.3:74341764-74341783 MS.gene75192:CDS 55.0%
GCTCAGTCTTGAACATCCCC+TGG + chr1.3:74344314-74344333 None:intergenic 55.0%
TCTGCAGGATGGTCCTTGCT+GGG - chr1.3:74344448-74344467 MS.gene75192:CDS 55.0%
TTGTTAGTTACGACGGCCGG+AGG + chr1.3:74342304-74342323 None:intergenic 55.0%
! AGATTGAGTGGAGGAAGCGC+GGG + chr1.3:74342359-74342378 None:intergenic 55.0%
! AGCGAGTAGCGCTTTTGGTG+CGG + chr1.3:74342336-74342355 None:intergenic 55.0%
!! CAGTTTTGTAGCGTGGGTGG+CGG + chr1.3:74344425-74344444 None:intergenic 55.0%
!! CCTCAGTTTTGTAGCGTGGG+TGG + chr1.3:74344428-74344447 None:intergenic 55.0%
CACGTCGCAGTGCCGTAATG+AGG - chr1.3:74343008-74343027 MS.gene75192:intron 60.0%
CTCATTACGGCACTGCGACG+TGG + chr1.3:74343010-74343029 None:intergenic 60.0%
CTCGCCGGAAACAGCAGCAA+CGG - chr1.3:74341779-74341798 MS.gene75192:CDS 60.0%
CTGCAGGATGGTCCTTGCTG+GGG - chr1.3:74344449-74344468 MS.gene75192:CDS 60.0%
CTGTTTCCGGCGAGCGATGA+TGG + chr1.3:74341773-74341792 None:intergenic 60.0%
GACAATACCGTGGGCGCATC+AGG + chr1.3:74342936-74342955 None:intergenic 60.0%
GGCGTTCGACTGCATGTGAC+CGG + chr1.3:74341708-74341727 None:intergenic 60.0%
GGTCACATGCAGTCGAACGC+CGG - chr1.3:74341707-74341726 MS.gene75192:CDS 60.0%
GTCTGCAGGATGGTCCTTGC+TGG - chr1.3:74344447-74344466 MS.gene75192:CDS 60.0%
TGCATCACCTGATGCGCCCA+CGG - chr1.3:74342926-74342945 MS.gene75192:intron 60.0%
TGTGCGAGGAGGGCAGAAAG+AGG - chr1.3:74344260-74344279 MS.gene75192:CDS 60.0%
TTAGTTACGACGGCCGGAGG+CGG + chr1.3:74342301-74342320 None:intergenic 60.0%
! CTTGAAGCTTGCTCCGCCTC+CGG - chr1.3:74342285-74342304 MS.gene75192:intron 60.0%
! GCCGATGCCGTAACCGCTTT+TGG + chr1.3:74341675-74341694 None:intergenic 60.0%
ACGGGCACACAACCCCAGCA+AGG + chr1.3:74344464-74344483 None:intergenic 65.0%
GCCGGAAACAGCAGCAACGG+CGG - chr1.3:74341782-74341801 MS.gene75192:CDS 65.0%
GCTGGGGTTGTGTGCCCGTA+TGG - chr1.3:74344465-74344484 MS.gene75192:CDS 65.0%
GGAAACAGCAGCAACGGCGG+CGG - chr1.3:74341785-74341804 MS.gene75192:CDS 65.0%
! GCGGGAGCGAGTAGCGCTTT+TGG + chr1.3:74342341-74342360 None:intergenic 65.0%
!! AGCGTGGGTGGCGGATCACT+GGG + chr1.3:74344416-74344435 None:intergenic 65.0%
!! GCCGCCGTTGCTGCTGTTTC+CGG + chr1.3:74341786-74341805 None:intergenic 65.0%
!! GGCATCGGCGTCAACAACGC+CGG - chr1.3:74341686-74341705 MS.gene75192:CDS 65.0%
!! TAGCGTGGGTGGCGGATCAC+TGG + chr1.3:74344417-74344436 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 74341641 74344575 74341641 ID=MS.gene75192
chr1.3 mRNA 74341641 74344575 74341641 ID=MS.gene75192.t1;Parent=MS.gene75192
chr1.3 exon 74343829 74344575 74343829 ID=MS.gene75192.t1.exon1;Parent=MS.gene75192.t1
chr1.3 CDS 74343829 74344575 74343829 ID=cds.MS.gene75192.t1;Parent=MS.gene75192.t1
chr1.3 exon 74341641 74341967 74341641 ID=MS.gene75192.t1.exon2;Parent=MS.gene75192.t1
chr1.3 CDS 74341641 74341967 74341641 ID=cds.MS.gene75192.t1;Parent=MS.gene75192.t1
Gene Sequence

>MS.gene75192

ATGCATAACAAGGACGTTTGTTCTCCGTCATCCAAAAGCGGTTACGGCATCGGCGTCAACAACGCCGGTCACATGCAGTCGAACGCCGGCGTGTTTGCTTCGTTGTACTCTGCTCTGAGTAATGATCTTCCATCATCGCTCGCCGGAAACAGCAGCAACGGCGGCGGATTTTCGCTTTATCCTTACTCTGACCTTGGAACAGAGTACGACTCATGCGTGATACAGAAGCATCAGGATATGGTGAATCGTCACAGTATGTTTCTCAGTCGTCTCCTGGAAAGTTCCAAAGAAGTTGAAGCTCTGCAGCAGGAAAACGGACAGCTCCGTGCCGTGAACAAAGAGTTGCAGAAGAATCTGAACCTTCTCGTTCAAGCTTCGCTGGAGAACCGATTCAATGGTGGCGGATCCTCCGTCCAGACTCAATCAACTCCGTTTGATGTGTTGCAAGGTTTCCGTGGCTTGAATCTTGGAGATGGAAAGGAAAACTGTGCTGATTGGAATAGCAATAACATCAATAATAATAACAAGGAGTTGCAGGAAGCTTCCGAATCTGATGAGAGTCCGACGAGTGTGATTGAGAATAACGGTGTTGAAACGGAGAGGTTCTCGCTTCCAAAGAGCATTTCTGTTAGATCCAATGGCTACTTGAAGCTTGCTCCGCCTCCGGCCGTCGTAACTAACAACAATGCTTGCCGCACCAAAAGCGCTACTCGCTCCCGCGCTTCCTCCACTCAATCTGATACAGTTGTAAGCACACGCACGTTATGATTGCTTTTATTTTATTTAATTAATTTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAATTTTTTTTTTTTATTATTATTGCTGCTATTGTTATTGTGATCTTAGCTTAATTTAATGTGTTATTATTGCTTTTATAGTTATAAACCAAATTTGACTTGCTTGTTAGTAGCTAATTATTTTCCTTCCAAATGAAGTAAAACCTTGCTTGTCTGTGTCATTACCTGTTAGCTGTAACAAACCTTGTAGTTTGGTTGTTTTTTCATTTGCGTGTGCTTTTTGCAAAGATGCAAGTTCGACTCCAGTTTATACTAGTTGTTGGTTAGTAGTGTTGCAGGACCAAATTAGCTCTAGTTTGCCATGAGGCAAAACAGATGAGCTAAACTTTGAAGCCTTTTTGTGACATTTTAACCTGTGTTCTGTGCCAATGTGCTCTGTATATGGTTTCAAATTGCGGATGCATCACCTGATGCGCCCACGGTATTGTCTGCAATCGCATCAAGCCGCAATTGTGGTGTGATCATAAATCACATTTGAAACCACGTCGCAGTGCCGTAATGAGGGAATTGGGTGCAGAAAACTAGTGATCACACAATTTATTTTTATACTTTACTTTATCTTTATATCATAGATTTAGAGAGTATTTTAAAGAACTAGCGATATTTTAAACTAATAGATGGCTGATATGAGATAAAGATTTTAGAGAGGAAACGGAAGATACATGAGCCATAAAATAAATAATAGTAAATTATAGTATTTATGCTAATAAAAATTAAGTTGACGCCACAAGGACGACAATACACATCGCAATAGCTGCAATATTGCAAAGGCAGCGACTGCAACAGCGGCCGCATTTTAAAACCATGGTTGTTTGTGCTTGGTGTCATGTTCCAATTAAGTTAAATTTTGATGCAGCCATACAGTTTTTACATTATAATTAAGGTGGTTAAATAATCTTTCTCACACTTCTCAATAAACTCTTGAAATAATATATAGAACTAGCAAGACTAGGAAGATTGATGAGTCATACAATGTCTACATTATAGGAGTATTTAAGAGGTCAAATATATACTATTTTTCTACATTTCTGAATTTTAAGACATTTATGATAGCCATGTATGGAAATTTGCAGTGCTGTTGGTAGAGGTTTGAAATCAATACCTTGGTTTTTCTAAGTTTTAAAGGGGATAAATGGTTGGACTGGTTTTACAAGTAACAAGTGATGCAGGGGATGCAGAATATGTCACTCAATTTCTTTTCATGGTTTTATCCACTGCTGTTGGTGTAGCTTTCAATAGACGTATAGTGCTGCTTATAGTTTGTCTCCATTTAGTTATTTGTTTTGTTACTCACTGCTGACTATGGTAATGTCTTGAATCTTCATTGGTTAGCCTTGGACACATCTTGTGCTTGGGATTAGCCTCTATTATTAATATATATGCTTTGATTTATTTATGTTGAAAATAAGGACAATACTTCGAGATAAAGATGAATGGATGTAAATAATTGTGTTCAATTTTGAAATGGTGTTTTCTTGAATGCCCTGCCTTGCCATTCCTTGACAGTCATACTAATGTTTCTTTCATCTTGACTGATTGTATTTTTCATCTTATTGTTTTTTTTATTTGATTCATATTCAAAATGCTCTGCCATGCCTTGACGCTCATAATAATGTAAAAGTAGATCACCGATTAATAACACTGAGTGGACATTATATAGTTTTGATTGTTGATATTCGGAAGAATTGATCATTGTTGAAATTTTGTGTTACTATACTGCAGCAAAAGGTATTTGTGCGAGGAGGGCAGAAAGAGGAAGAGCCTCTTGAGATGGTTGTGTACAACCAGGGGATGTTCAAGACTGAGCTCTGTAACAAATGGCAGGAAACTGGCACATGCCCTTATGGAGACCACTGTCAATTCGCTCATGGCATTGGGGAGCTTCGCCCAGTGATCCGCCACCCACGCTACAAAACTGAGGTCTGCAGGATGGTCCTTGCTGGGGTTGTGTGCCCGTATGGCCATAGATGCCATTTCCGTCATGCACTTACTGAACAAGAGAAAGCTGTGTCACAATCTAAGCCCAGATCAATGAAGCTGGAAAGATGA
Protein sequence

>MS.gene75192.t1

MHNKDVCSPSSKSGYGIGVNNAGHMQSNAGVFASLYSALSNDLPSSLAGNSSNGGGFSLYPYSDLGTEYDSCVIQKHQDMVNRHSMFLSRLLESSKEVEALQQENGQLRAVNKELQKNLNLLVQASLENRFNGGGSSVQTQSTPFDVLQGFRGLNLGDGKENCADWNSNNINNNNKELQEASESDESPTSVIENNGVETERFSLPKSISVRSNGYLKLAPPPAVVTNNNACRTKSATRSRASSTQSDTVQKVFVRGGQKEEEPLEMVVYNQGMFKTELCNKWQETGTCPYGDHCQFAHGIGELRPVIRHPRYKTEVCRMVLAGVVCPYGHRCHFRHALTEQEKAVSQSKPRSMKLER