AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75482

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75482.t1 MTR_2g093960 35.393 178 99 5 104 272 37 207 2.50e-28 113
MS.gene75482.t1 MTR_8g017210 35.897 156 92 3 104 259 67 214 4.79e-27 111
MS.gene75482.t1 MTR_4g059870 35.897 156 93 3 104 259 43 191 5.03e-26 108
MS.gene75482.t1 MTR_2g099990 35.897 156 92 3 104 259 51 198 2.01e-25 106
MS.gene75482.t1 MTR_1g094115 35.762 151 85 4 111 259 59 199 1.35e-24 104
MS.gene75482.t1 MTR_1g112270 34.437 151 87 4 111 259 56 196 1.47e-24 104
MS.gene75482.t1 MTR_1g112270 34.437 151 87 4 111 259 67 207 1.81e-24 103
MS.gene75482.t1 MTR_1g016010 35.099 151 91 3 109 259 51 194 6.01e-24 102
MS.gene75482.t1 MTR_2g090745 35.570 149 88 3 111 259 19 159 3.40e-23 99.8
MS.gene75482.t1 MTR_2g099990 37.226 137 78 3 123 259 1 129 1.26e-22 97.8
MS.gene75482.t1 MTR_1g093095 36.364 165 90 7 130 287 117 273 2.86e-21 92.4
MS.gene75482.t1 MTR_1g094115 36.842 133 72 4 129 259 60 182 4.15e-21 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75482.t1 AT1G34370 58.788 165 62 1 113 271 19 183 2.76e-70 220
MS.gene75482.t1 AT1G34370 39.748 317 120 6 26 271 77 393 3.60e-69 224
MS.gene75482.t1 AT1G34370 39.748 317 120 6 26 271 77 393 3.60e-69 224
MS.gene75482.t1 AT5G22890 58.750 160 60 1 113 266 202 361 3.34e-66 213
MS.gene75482.t1 AT5G66730 37.179 156 90 3 104 259 37 184 1.70e-27 112
MS.gene75482.t1 AT5G44160 35.256 156 93 3 104 259 42 189 2.17e-27 111
MS.gene75482.t1 AT5G44160 35.762 151 89 3 109 259 8 150 2.62e-27 111
MS.gene75482.t1 AT1G03840 36.424 151 88 3 109 259 51 193 2.69e-27 112
MS.gene75482.t1 AT1G03840 36.242 149 87 3 111 259 51 191 1.09e-26 110
MS.gene75482.t1 AT5G03150 35.065 154 93 4 106 259 60 206 7.33e-26 107
MS.gene75482.t1 AT3G45260 35.762 151 90 3 109 259 49 192 8.99e-26 107
MS.gene75482.t1 AT3G45260 35.762 151 90 3 109 259 49 192 8.99e-26 107
MS.gene75482.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.13e-25 107
MS.gene75482.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.13e-25 107
MS.gene75482.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.13e-25 107
MS.gene75482.t1 AT5G60470 35.294 153 92 3 104 256 49 194 1.33e-25 106
MS.gene75482.t1 AT5G60470 35.294 153 92 3 104 256 45 190 1.63e-25 106
MS.gene75482.t1 AT3G50700 35.897 156 92 3 104 259 39 186 6.83e-25 104
MS.gene75482.t1 AT5G60470 35.616 146 87 3 111 256 47 185 8.10e-25 104
MS.gene75482.t1 AT3G13810 36.364 154 91 4 109 262 75 221 8.92e-25 104
MS.gene75482.t1 AT3G13810 36.364 154 91 4 109 262 61 207 9.85e-25 104
MS.gene75482.t1 AT3G13810 36.364 154 91 4 109 262 80 226 9.97e-25 104
MS.gene75482.t1 AT3G13810 36.364 154 91 4 109 262 80 226 9.97e-25 104
MS.gene75482.t1 AT2G02070 33.333 159 96 4 101 259 56 204 4.05e-24 103
MS.gene75482.t1 AT2G02070 33.333 159 96 4 101 259 56 204 4.05e-24 103
MS.gene75482.t1 AT4G02670 36.424 151 89 3 109 259 65 208 5.57e-24 101
MS.gene75482.t1 AT3G13810 36.184 152 90 4 111 262 83 227 6.08e-24 102
MS.gene75482.t1 AT4G02670 36.424 151 89 3 109 259 63 206 6.26e-24 101
MS.gene75482.t1 AT3G13810 36.184 152 90 4 111 262 69 213 6.71e-24 102
MS.gene75482.t1 AT1G14580 28.934 197 117 5 78 259 17 205 8.37e-24 101
MS.gene75482.t1 AT1G14580 28.934 197 117 5 78 259 17 205 8.37e-24 101
MS.gene75482.t1 AT1G14580 28.934 197 117 5 78 259 31 219 1.12e-23 101
MS.gene75482.t1 AT2G02080 32.886 149 92 3 111 259 66 206 2.34e-23 100
MS.gene75482.t1 AT2G02080 32.886 149 92 3 111 259 66 206 2.34e-23 100
MS.gene75482.t1 AT2G02080 32.886 149 92 3 111 259 66 206 2.34e-23 100
MS.gene75482.t1 AT2G02080 32.886 149 92 3 111 259 66 206 2.34e-23 100
MS.gene75482.t1 AT1G51220 39.860 143 78 7 130 268 180 318 3.55e-23 98.2
MS.gene75482.t1 AT3G20880 35.849 159 94 6 130 284 258 412 1.43e-22 97.8
MS.gene75482.t1 AT1G08290 35.625 160 92 6 130 282 182 337 5.43e-22 95.1
MS.gene75482.t1 AT1G34790 40.000 150 81 7 121 265 137 282 8.98e-22 94.0
MS.gene75482.t1 AT2G01940 35.570 149 88 4 109 256 54 195 4.42e-21 93.6
MS.gene75482.t1 AT1G25250 36.913 149 87 4 109 256 20 162 5.81e-21 92.4
MS.gene75482.t1 AT1G25250 36.913 149 87 4 109 256 43 185 6.20e-21 92.8
MS.gene75482.t1 AT1G13290 33.333 195 121 6 81 270 55 245 8.20e-21 91.3
MS.gene75482.t1 AT1G68130 35.570 149 88 4 109 256 51 192 3.26e-20 90.9
MS.gene75482.t1 AT2G02080 32.117 137 85 3 123 259 1 129 5.49e-20 90.5
MS.gene75482.t1 AT2G02080 32.117 137 85 3 123 259 1 129 5.49e-20 90.5
MS.gene75482.t1 AT3G57670 32.353 204 113 8 109 299 189 380 6.57e-20 89.7
MS.gene75482.t1 AT2G01940 35.766 137 80 4 109 244 54 183 1.91e-18 85.9

Find 100 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTTCTTCCATGATTT+TGG 0.088932 3.3:-72674216 MS.gene75482:CDS
TGGGTAGTGTGATGGGAATC+TGG 0.233101 3.3:+72675101 None:intergenic
CTATTGCTTCGATGAAGTTT+TGG 0.237371 3.3:-72674181 MS.gene75482:CDS
GGACGACGGAGCACGCTTAA+AGG 0.284022 3.3:+72674630 None:intergenic
TTTGCTGTGGATGAAGTATC+TGG 0.296757 3.3:+72675081 None:intergenic
AAAGGCTTTGCTAAAGCTTC+CGG 0.321455 3.3:+72674648 None:intergenic
TGGAGTAAAGATGATGAAAT+TGG 0.348705 3.3:-72674246 MS.gene75482:CDS
GAAGAATTATTCGATGCTTT+CGG 0.349953 3.3:-72674463 MS.gene75482:CDS
AACGCCGCCGCCCTCGTTCC+CGG 0.371943 3.3:-72674879 MS.gene75482:CDS
TCTAGGAAGGATAAGTTGTT+TGG 0.372634 3.3:-72674375 MS.gene75482:CDS
TGAGGAGAATGAAGATCTTA+TGG 0.375131 3.3:-72674277 MS.gene75482:CDS
GAGGGCGGCGGCGTTGACTA+TGG 0.385405 3.3:+72674887 None:intergenic
GGTGGAGGACGGCAAAGATT+TGG 0.403975 3.3:+72674913 None:intergenic
GGACATGTTGCTTTGTTTGA+GGG 0.416752 3.3:-72674354 MS.gene75482:CDS
CGAAGCGGAACTTGAGGGTC+GGG 0.417024 3.3:+72675044 None:intergenic
GAAATTCAAAGCTTTGAAAT+CGG 0.425461 3.3:-72674556 MS.gene75482:CDS
GGAATCTTCCACGGCGGTGA+GGG 0.432442 3.3:+72674809 None:intergenic
GAGCGTTGGTGGTGATGGAT+TGG 0.440346 3.3:+72674970 None:intergenic
TGCCGCATATCTCGCAGAAA+TGG 0.442862 3.3:+72674736 None:intergenic
TGGACATGTTGCTTTGTTTG+AGG 0.450086 3.3:-72674355 MS.gene75482:CDS
TCCGCTTCGGAATCTATCCT+TGG 0.455820 3.3:-72675030 MS.gene75482:CDS
TCAAGCGCGACGCGAATCTC+CGG 0.460817 3.3:-72674707 MS.gene75482:CDS
CGGATTTGAAGAGTCATATG+AGG 0.460927 3.3:-72674443 MS.gene75482:CDS
GGCACCGGGAACGAGGGCGG+CGG 0.463287 3.3:+72674875 None:intergenic
GATTCCGAAGCGGAACTTGA+GGG 0.464978 3.3:+72675039 None:intergenic
GGAGGAGAAGACGGAGGCAC+CGG 0.465619 3.3:+72674860 None:intergenic
TATCAATAGACCCAAAATCA+TGG 0.476438 3.3:+72674205 None:intergenic
AGCGTTGGTGGTGATGGATT+GGG 0.484580 3.3:+72674971 None:intergenic
CCTGCTGTTGTGTTGGATGA+TGG 0.487310 3.3:-72674324 MS.gene75482:CDS
GAATCTTCCACGGCGGTGAG+GGG 0.490348 3.3:+72674810 None:intergenic
GGGTAGTGTGATGGGAATCT+GGG 0.490735 3.3:+72675102 None:intergenic
AAGTATCTGGGTAGTGTGAT+GGG 0.491876 3.3:+72675094 None:intergenic
CGTGAGCTCTCATGTGCATC+CGG 0.492318 3.3:+72674688 None:intergenic
AGGGAAGCAAGTTGTGGCTG+AGG 0.495886 3.3:-72674295 MS.gene75482:CDS
GACGGAGGCACCGGGAACGA+GGG 0.499392 3.3:+72674869 None:intergenic
CTAAGTGGAAGTGTTCTTGT+GGG 0.500097 3.3:-72674407 MS.gene75482:CDS
GACAGGAGCGTTGGTGGTGA+TGG 0.501841 3.3:+72674965 None:intergenic
TGGATTGGGACAGGAAGATT+CGG 0.505176 3.3:+72674985 None:intergenic
GTGTGATGGGAATCTGGGTT+CGG 0.507152 3.3:+72675107 None:intergenic
AGATTACATGAATACATCTT+CGG 0.508237 3.3:+72674495 None:intergenic
TGGTGGTGATGGATTGGGAC+AGG 0.519067 3.3:+72674976 None:intergenic
AATCTTCCACGGCGGTGAGG+GGG 0.520341 3.3:+72674811 None:intergenic
TAAGCGTGCTCCGTCGTCCA+AGG 0.521046 3.3:-72674626 MS.gene75482:CDS
TAAAGATGATGAAATTGGTA+TGG 0.524053 3.3:-72674241 MS.gene75482:CDS
ACGAGAACCGCCTTGGACGA+CGG 0.524869 3.3:+72674616 None:intergenic
TCATATGCCTGCTGTTGTGT+TGG 0.531256 3.3:-72674331 MS.gene75482:CDS
GTTTCTGTTACATCCTTCGA+AGG 0.532042 3.3:+72674590 None:intergenic
GAGCTCCACAATTTCACAAT+CGG 0.535509 3.3:+72674788 None:intergenic
TTCTGCGAGATATGCGGCAA+AGG 0.541688 3.3:-72674732 MS.gene75482:CDS
AAGGGACACGAGAACCGCCT+TGG 0.542108 3.3:+72674609 None:intergenic
GTTAATTGATCATTGCTGAC+AGG 0.542229 3.3:+72674948 None:intergenic
GGAGGCACCGGGAACGAGGG+CGG 0.542744 3.3:+72674872 None:intergenic
GAGGAGAAGACGGAGGCACC+GGG 0.543680 3.3:+72674861 None:intergenic
CCGACCCTCAAGTTCCGCTT+CGG 0.543888 3.3:-72675043 MS.gene75482:CDS
CCATCATCCAACACAACAGC+AGG 0.544109 3.3:+72674324 None:intergenic
GAGGACGGCAAAGATTTGGT+TGG 0.549075 3.3:+72674917 None:intergenic
GTGTTGGATGATGGAGAGAA+AGG 0.549221 3.3:-72674315 MS.gene75482:CDS
AGACGGAGGCACCGGGAACG+AGG 0.549677 3.3:+72674868 None:intergenic
GTGTGAAGACTCATTTCAAG+AGG 0.550074 3.3:-72674527 MS.gene75482:CDS
GAAGCGGAACTTGAGGGTCG+GGG 0.550186 3.3:+72675045 None:intergenic
ATTGCTGACAGGAGCGTTGG+TGG 0.551289 3.3:+72674959 None:intergenic
GAATGAAGATCTTATGGAGA+TGG 0.552087 3.3:-72674271 MS.gene75482:CDS
CGGAATCTTCCACGGCGGTG+AGG 0.555882 3.3:+72674808 None:intergenic
AGATTCCGAAGCGGAACTTG+AGG 0.557075 3.3:+72675038 None:intergenic
GGCAAAGATTTGGTTGGAGA+CGG 0.557743 3.3:+72674923 None:intergenic
CGGTTCTCGTGTCCCTTCGA+AGG 0.558125 3.3:-72674603 MS.gene75482:CDS
CGGATGCACATGAGAGCTCA+CGG 0.562771 3.3:-72674687 MS.gene75482:CDS
GGCAATGTGGCGAGTCTAAG+TGG 0.563877 3.3:-72674422 MS.gene75482:CDS
AAGCGGAACTTGAGGGTCGG+GGG 0.565688 3.3:+72675046 None:intergenic
GAAGTATCTGGGTAGTGTGA+TGG 0.565886 3.3:+72675093 None:intergenic
AAAGGTGATGGAGGAGAAGA+CGG 0.568206 3.3:+72674851 None:intergenic
TCTAAGTGGAAGTGTTCTTG+TGG 0.572655 3.3:-72674408 MS.gene75482:CDS
ATCATTGCTGACAGGAGCGT+TGG 0.573157 3.3:+72674956 None:intergenic
AATTGTGGAGCTCGACGCCA+TGG 0.578523 3.3:-72674778 MS.gene75482:CDS
TTCACAATCGGAATCTTCCA+CGG 0.580558 3.3:+72674800 None:intergenic
TGTTGGATGATGGAGAGAAA+GGG 0.582040 3.3:-72674314 MS.gene75482:CDS
AAGATCTTATGGAGATGGCA+TGG 0.583976 3.3:-72674266 MS.gene75482:CDS
CGGCGGCGTTGACTATGGTA+TGG 0.588110 3.3:+72674892 None:intergenic
GTTGTTGTTGACTGAATCAA+AGG 0.592805 3.3:+72674833 None:intergenic
AGATTCCGATTGTGAAATTG+TGG 0.593692 3.3:-72674793 MS.gene75482:CDS
ATGTTCAGCTAGCAATTCCA+TGG 0.594022 3.3:+72674761 None:intergenic
TTGCTGTGGATGAAGTATCT+GGG 0.597399 3.3:+72675082 None:intergenic
CCGAAGCGGAACTTGAGGGT+CGG 0.601485 3.3:+72675043 None:intergenic
GGATGCACATGAGAGCTCAC+GGG 0.604619 3.3:-72674686 MS.gene75482:CDS
GACTATGGTATGGTGGAGGA+CGG 0.605416 3.3:+72674902 None:intergenic
TTTCTGTTACATCCTTCGAA+GGG 0.617127 3.3:+72674591 None:intergenic
CTCCATTTCTGCGAGATATG+CGG 0.627013 3.3:-72674738 MS.gene75482:CDS
AAAGATTTGGTTGGAGACGG+CGG 0.627067 3.3:+72674926 None:intergenic
GTTGACTGAATCAAAGGTGA+TGG 0.628225 3.3:+72674839 None:intergenic
CGGCGTTGACTATGGTATGG+TGG 0.628323 3.3:+72674895 None:intergenic
CGTTGACTATGGTATGGTGG+AGG 0.634509 3.3:+72674898 None:intergenic
AGAGAAAGGGAAGCAAGTTG+TGG 0.634835 3.3:-72674301 MS.gene75482:CDS
GACTGAATCAAAGGTGATGG+AGG 0.635823 3.3:+72674842 None:intergenic
ATCGATGCTTGTCTGAACCA+AGG 0.654173 3.3:+72675013 None:intergenic
CGGGAATCAATTCAAGACGC+CGG 0.656722 3.3:-72674667 MS.gene75482:CDS
AAGAGTCATATGAGGCAATG+TGG 0.696717 3.3:-72674435 MS.gene75482:CDS
GCGTGCTCCGTCGTCCAAGG+CGG 0.710450 3.3:-72674623 MS.gene75482:CDS
ACCAAGGATAGATTCCGAAG+CGG 0.716599 3.3:+72675029 None:intergenic
ACAATCGGAATCTTCCACGG+CGG 0.728128 3.3:+72674803 None:intergenic
GGTGATGGAGGAGAAGACGG+AGG 0.738851 3.3:+72674854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGATTACATGAATACATCTT+CGG + chr3.3:72674788-72674807 None:intergenic 25.0%
! TAAAGATGATGAAATTGGTA+TGG - chr3.3:72675039-72675058 MS.gene75482:CDS 25.0%
!!! GAAATTCAAAGCTTTGAAAT+CGG - chr3.3:72674724-72674743 MS.gene75482:CDS 25.0%
GAAGAATTATTCGATGCTTT+CGG - chr3.3:72674817-72674836 MS.gene75482:CDS 30.0%
TATCAATAGACCCAAAATCA+TGG + chr3.3:72675078-72675097 None:intergenic 30.0%
TGGAGTAAAGATGATGAAAT+TGG - chr3.3:72675034-72675053 MS.gene75482:CDS 30.0%
! AAGAGTTCTTCCATGATTTT+GGG - chr3.3:72675065-72675084 MS.gene75482:CDS 30.0%
! TATTGCTTCGATGAAGTTTT+GGG - chr3.3:72675100-72675119 MS.gene75482:CDS 30.0%
AGATTCCGATTGTGAAATTG+TGG - chr3.3:72674487-72674506 MS.gene75482:CDS 35.0%
GAATGAAGATCTTATGGAGA+TGG - chr3.3:72675009-72675028 MS.gene75482:CDS 35.0%
GTTAATTGATCATTGCTGAC+AGG + chr3.3:72674335-72674354 None:intergenic 35.0%
TCTAGGAAGGATAAGTTGTT+TGG - chr3.3:72674905-72674924 MS.gene75482:CDS 35.0%
TGAGGAGAATGAAGATCTTA+TGG - chr3.3:72675003-72675022 MS.gene75482:CDS 35.0%
TTTCTGTTACATCCTTCGAA+GGG + chr3.3:72674692-72674711 None:intergenic 35.0%
! CTATTGCTTCGATGAAGTTT+TGG - chr3.3:72675099-72675118 MS.gene75482:CDS 35.0%
! GAAGAGTTCTTCCATGATTT+TGG - chr3.3:72675064-72675083 MS.gene75482:CDS 35.0%
! GTATTAGTTGGATTTTGCTG+TGG + chr3.3:72674215-72674234 None:intergenic 35.0%
! GTTGTTGTTGACTGAATCAA+AGG + chr3.3:72674450-72674469 None:intergenic 35.0%
AAAGGCTTTGCTAAAGCTTC+CGG + chr3.3:72674635-72674654 None:intergenic 40.0%
AAGAGTCATATGAGGCAATG+TGG - chr3.3:72674845-72674864 MS.gene75482:CDS 40.0%
AAGATCTTATGGAGATGGCA+TGG - chr3.3:72675014-72675033 MS.gene75482:CDS 40.0%
AAGTATCTGGGTAGTGTGAT+GGG + chr3.3:72674189-72674208 None:intergenic 40.0%
ATGTTCAGCTAGCAATTCCA+TGG + chr3.3:72674522-72674541 None:intergenic 40.0%
CGGATTTGAAGAGTCATATG+AGG - chr3.3:72674837-72674856 MS.gene75482:CDS 40.0%
GAGCTCCACAATTTCACAAT+CGG + chr3.3:72674495-72674514 None:intergenic 40.0%
GTGTGAAGACTCATTTCAAG+AGG - chr3.3:72674753-72674772 MS.gene75482:CDS 40.0%
GTTGACTGAATCAAAGGTGA+TGG + chr3.3:72674444-72674463 None:intergenic 40.0%
GTTTCTGTTACATCCTTCGA+AGG + chr3.3:72674693-72674712 None:intergenic 40.0%
TTCACAATCGGAATCTTCCA+CGG + chr3.3:72674483-72674502 None:intergenic 40.0%
! TTGCTGTGGATGAAGTATCT+GGG + chr3.3:72674201-72674220 None:intergenic 40.0%
! TTTGCTGTGGATGAAGTATC+TGG + chr3.3:72674202-72674221 None:intergenic 40.0%
!! CTAAGTGGAAGTGTTCTTGT+GGG - chr3.3:72674873-72674892 MS.gene75482:CDS 40.0%
!! GGACATGTTGCTTTGTTTGA+GGG - chr3.3:72674926-72674945 MS.gene75482:CDS 40.0%
!! TCTAAGTGGAAGTGTTCTTG+TGG - chr3.3:72674872-72674891 MS.gene75482:CDS 40.0%
!! TGGACATGTTGCTTTGTTTG+AGG - chr3.3:72674925-72674944 MS.gene75482:CDS 40.0%
!! TGTTGGATGATGGAGAGAAA+GGG - chr3.3:72674966-72674985 MS.gene75482:CDS 40.0%
!!! CTTGTGGGAGTACTTTTTCT+AGG - chr3.3:72674888-72674907 MS.gene75482:CDS 40.0%
!!! GATGAAGTTTTGGGATTTCG+TGG - chr3.3:72675109-72675128 MS.gene75482:CDS 40.0%
!!! TGGGAGTACTTTTTCTAGGA+AGG - chr3.3:72674892-72674911 MS.gene75482:CDS 40.0%
ACCAAGGATAGATTCCGAAG+CGG + chr3.3:72674254-72674273 None:intergenic 45.0%
AGAGAAAGGGAAGCAAGTTG+TGG - chr3.3:72674979-72674998 MS.gene75482:CDS 45.0%
ATCGATGCTTGTCTGAACCA+AGG + chr3.3:72674270-72674289 None:intergenic 45.0%
CTCCATTTCTGCGAGATATG+CGG - chr3.3:72674542-72674561 MS.gene75482:CDS 45.0%
TCATATGCCTGCTGTTGTGT+TGG - chr3.3:72674949-72674968 MS.gene75482:CDS 45.0%
! AAAGATTTGGTTGGAGACGG+CGG + chr3.3:72674357-72674376 None:intergenic 45.0%
! GAAGTATCTGGGTAGTGTGA+TGG + chr3.3:72674190-72674209 None:intergenic 45.0%
! GACTGAATCAAAGGTGATGG+AGG + chr3.3:72674441-72674460 None:intergenic 45.0%
! GGCAAAGATTTGGTTGGAGA+CGG + chr3.3:72674360-72674379 None:intergenic 45.0%
! TGGATTGGGACAGGAAGATT+CGG + chr3.3:72674298-72674317 None:intergenic 45.0%
!! AAAGGTGATGGAGGAGAAGA+CGG + chr3.3:72674432-72674451 None:intergenic 45.0%
!! GTGTTGGATGATGGAGAGAA+AGG - chr3.3:72674965-72674984 MS.gene75482:CDS 45.0%
ACAATCGGAATCTTCCACGG+CGG + chr3.3:72674480-72674499 None:intergenic 50.0%
AGATTCCGAAGCGGAACTTG+AGG + chr3.3:72674245-72674264 None:intergenic 50.0%
CCATCATCCAACACAACAGC+AGG + chr3.3:72674959-72674978 None:intergenic 50.0%
CCTGCTGTTGTGTTGGATGA+TGG - chr3.3:72674956-72674975 MS.gene75482:CDS 50.0%
CGGGAATCAATTCAAGACGC+CGG - chr3.3:72674613-72674632 MS.gene75482:CDS 50.0%
GATTCCGAAGCGGAACTTGA+GGG + chr3.3:72674244-72674263 None:intergenic 50.0%
TCCGCTTCGGAATCTATCCT+TGG - chr3.3:72674250-72674269 MS.gene75482:CDS 50.0%
TGCCGCATATCTCGCAGAAA+TGG + chr3.3:72674547-72674566 None:intergenic 50.0%
TTCTGCGAGATATGCGGCAA+AGG - chr3.3:72674548-72674567 MS.gene75482:CDS 50.0%
! ATCATTGCTGACAGGAGCGT+TGG + chr3.3:72674327-72674346 None:intergenic 50.0%
! CGTTGACTATGGTATGGTGG+AGG + chr3.3:72674385-72674404 None:intergenic 50.0%
! GACTATGGTATGGTGGAGGA+CGG + chr3.3:72674381-72674400 None:intergenic 50.0%
! GAGGACGGCAAAGATTTGGT+TGG + chr3.3:72674366-72674385 None:intergenic 50.0%
! GGGTAGTGTGATGGGAATCT+GGG + chr3.3:72674181-72674200 None:intergenic 50.0%
! GTGTGATGGGAATCTGGGTT+CGG + chr3.3:72674176-72674195 None:intergenic 50.0%
! TGGGTAGTGTGATGGGAATC+TGG + chr3.3:72674182-72674201 None:intergenic 50.0%
!! AGCGTTGGTGGTGATGGATT+GGG + chr3.3:72674312-72674331 None:intergenic 50.0%
AATTGTGGAGCTCGACGCCA+TGG - chr3.3:72674502-72674521 MS.gene75482:CDS 55.0%
AGGGAAGCAAGTTGTGGCTG+AGG - chr3.3:72674985-72675004 MS.gene75482:CDS 55.0%
CGGATGCACATGAGAGCTCA+CGG - chr3.3:72674593-72674612 MS.gene75482:CDS 55.0%
CGTGAGCTCTCATGTGCATC+CGG + chr3.3:72674595-72674614 None:intergenic 55.0%
GGATGCACATGAGAGCTCAC+GGG - chr3.3:72674594-72674613 MS.gene75482:CDS 55.0%
GGCAATGTGGCGAGTCTAAG+TGG - chr3.3:72674858-72674877 MS.gene75482:CDS 55.0%
GGTGGAGGACGGCAAAGATT+TGG + chr3.3:72674370-72674389 None:intergenic 55.0%
!! ATTGCTGACAGGAGCGTTGG+TGG + chr3.3:72674324-72674343 None:intergenic 55.0%
!! CGGCGTTGACTATGGTATGG+TGG + chr3.3:72674388-72674407 None:intergenic 55.0%
!! GAGCGTTGGTGGTGATGGAT+TGG + chr3.3:72674313-72674332 None:intergenic 55.0%
!! TGAGGGTCGGGGGTATTAGT+TGG + chr3.3:72674227-72674246 None:intergenic 55.0%
!! TGGTGGTGATGGATTGGGAC+AGG + chr3.3:72674307-72674326 None:intergenic 55.0%
AAGCGGAACTTGAGGGTCGG+GGG + chr3.3:72674237-72674256 None:intergenic 60.0%
AAGGGACACGAGAACCGCCT+TGG + chr3.3:72674674-72674693 None:intergenic 60.0%
AATCTTCCACGGCGGTGAGG+GGG + chr3.3:72674472-72674491 None:intergenic 60.0%
ACGAGAACCGCCTTGGACGA+CGG + chr3.3:72674667-72674686 None:intergenic 60.0%
CCGAAGCGGAACTTGAGGGT+CGG + chr3.3:72674240-72674259 None:intergenic 60.0%
CCGACCCTCAAGTTCCGCTT+CGG - chr3.3:72674237-72674256 MS.gene75482:CDS 60.0%
CGAAGCGGAACTTGAGGGTC+GGG + chr3.3:72674239-72674258 None:intergenic 60.0%
CGGTTCTCGTGTCCCTTCGA+AGG - chr3.3:72674677-72674696 MS.gene75482:CDS 60.0%
GAAGCGGAACTTGAGGGTCG+GGG + chr3.3:72674238-72674257 None:intergenic 60.0%
GAATCTTCCACGGCGGTGAG+GGG + chr3.3:72674473-72674492 None:intergenic 60.0%
GGAATCTTCCACGGCGGTGA+GGG + chr3.3:72674474-72674493 None:intergenic 60.0%
GGACGACGGAGCACGCTTAA+AGG + chr3.3:72674653-72674672 None:intergenic 60.0%
TAAGCGTGCTCCGTCGTCCA+AGG - chr3.3:72674654-72674673 MS.gene75482:CDS 60.0%
TCAAGCGCGACGCGAATCTC+CGG - chr3.3:72674573-72674592 MS.gene75482:CDS 60.0%
!! CGGCGGCGTTGACTATGGTA+TGG + chr3.3:72674391-72674410 None:intergenic 60.0%
!! GACAGGAGCGTTGGTGGTGA+TGG + chr3.3:72674318-72674337 None:intergenic 60.0%
!! GGTGATGGAGGAGAAGACGG+AGG + chr3.3:72674429-72674448 None:intergenic 60.0%
CGGAATCTTCCACGGCGGTG+AGG + chr3.3:72674475-72674494 None:intergenic 65.0%
! GGAGGAGAAGACGGAGGCAC+CGG + chr3.3:72674423-72674442 None:intergenic 65.0%
!! GAGGAGAAGACGGAGGCACC+GGG + chr3.3:72674422-72674441 None:intergenic 65.0%
CAACAACCCCCTCACCGCCG+TGG - chr3.3:72674463-72674482 MS.gene75482:CDS 70.0%
GCGTGCTCCGTCGTCCAAGG+CGG - chr3.3:72674657-72674676 MS.gene75482:CDS 70.0%
!! AGACGGAGGCACCGGGAACG+AGG + chr3.3:72674415-72674434 None:intergenic 70.0%
!! GACGGAGGCACCGGGAACGA+GGG + chr3.3:72674414-72674433 None:intergenic 70.0%
!! GAGGGCGGCGGCGTTGACTA+TGG + chr3.3:72674396-72674415 None:intergenic 70.0%
AACGCCGCCGCCCTCGTTCC+CGG - chr3.3:72674401-72674420 MS.gene75482:CDS 75.0%
!! GGAGGCACCGGGAACGAGGG+CGG + chr3.3:72674411-72674430 None:intergenic 75.0%
!! GGCACCGGGAACGAGGGCGG+CGG + chr3.3:72674408-72674427 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 72674164 72675138 72674164 ID=MS.gene75482
chr3.3 mRNA 72674164 72675138 72674164 ID=MS.gene75482.t1;Parent=MS.gene75482
chr3.3 exon 72674164 72675138 72674164 ID=MS.gene75482.t1.exon1;Parent=MS.gene75482.t1
chr3.3 CDS 72674164 72675138 72674164 ID=cds.MS.gene75482.t1;Parent=MS.gene75482.t1
Gene Sequence

>MS.gene75482

ATGTCTAATCCGAACCCAGATTCCCATCACACTACCCAGATACTTCATCCACAGCAAAATCCAACTAATACCCCCGACCCTCAAGTTCCGCTTCGGAATCTATCCTTGGTTCAGACAAGCATCGATTCTCTCCGAATCTTCCTGTCCCAATCCATCACCACCAACGCTCCTGTCAGCAATGATCAATTAACCGCCGTCTCCAACCAAATCTTTGCCGTCCTCCACCATACCATAGTCAACGCCGCCGCCCTCGTTCCCGGTGCCTCCGTCTTCTCCTCCATCACCTTTGATTCAGTCAACAACAACCCCCTCACCGCCGTGGAAGATTCCGATTGTGAAATTGTGGAGCTCGACGCCATGGAATTGCTAGCTGAACATCTCCATTTCTGCGAGATATGCGGCAAAGGCTTCAAGCGCGACGCGAATCTCCGGATGCACATGAGAGCTCACGGGAATCAATTCAAGACGCCGGAAGCTTTAGCAAAGCCTTTAAGCGTGCTCCGTCGTCCAAGGCGGTTCTCGTGTCCCTTCGAAGGATGTAACAGAAACAAGAAGCATAAGAAATTCAAAGCTTTGAAATCGGTGATTTGTGTGAAGACTCATTTCAAGAGGAGTCATTGTCCGAAGATGTATTCATGTAATCTTTGTCGTAAGAAGAATTATTCGATGCTTTCGGATTTGAAGAGTCATATGAGGCAATGTGGCGAGTCTAAGTGGAAGTGTTCTTGTGGGAGTACTTTTTCTAGGAAGGATAAGTTGTTTGGACATGTTGCTTTGTTTGAGGGTCATATGCCTGCTGTTGTGTTGGATGATGGAGAGAAAGGGAAGCAAGTTGTGGCTGAGGAGAATGAAGATCTTATGGAGATGGCATGGAGTAAAGATGATGAAATTGGTATGGATGAAGAGTTCTTCCATGATTTTGGGTCTATTGATAACTATTGCTTCGATGAAGTTTTGGGATTTCGTGGTAGCTAG
Protein sequence

>MS.gene75482.t1

MSNPNPDSHHTTQILHPQQNPTNTPDPQVPLRNLSLVQTSIDSLRIFLSQSITTNAPVSNDQLTAVSNQIFAVLHHTIVNAAALVPGASVFSSITFDSVNNNPLTAVEDSDCEIVELDAMELLAEHLHFCEICGKGFKRDANLRMHMRAHGNQFKTPEALAKPLSVLRRPRRFSCPFEGCNRNKKHKKFKALKSVICVKTHFKRSHCPKMYSCNLCRKKNYSMLSDLKSHMRQCGESKWKCSCGSTFSRKDKLFGHVALFEGHMPAVVLDDGEKGKQVVAEENEDLMEMAWSKDDEIGMDEEFFHDFGSIDNYCFDEVLGFRGS