AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75620.t1 MTR_3g085180 96.774 434 14 0 1 434 1 434 0.0 870
MS.gene75620.t1 MTR_8g080670 52.518 417 167 8 1 399 1 404 4.85e-113 338
MS.gene75620.t1 MTR_8g080680 48.174 438 173 8 1 425 1 397 8.22e-113 337
MS.gene75620.t1 MTR_8g080690 52.152 395 160 9 1 385 1 376 1.18e-107 323
MS.gene75620.t1 MTR_1g053715 39.695 262 145 5 115 368 18 274 5.93e-41 149
MS.gene75620.t1 MTR_7g092930 39.623 265 137 7 120 374 17 268 1.70e-40 147
MS.gene75620.t1 MTR_5g046670 41.827 208 112 4 39 240 115 319 5.99e-40 149
MS.gene75620.t1 MTR_7g444860 48.447 161 79 3 93 249 73 233 4.23e-38 140
MS.gene75620.t1 MTR_8g463140 70.000 80 24 0 163 242 63 142 5.94e-36 130
MS.gene75620.t1 MTR_4g109770 51.562 128 55 2 117 240 74 198 1.11e-35 137
MS.gene75620.t1 MTR_3g099080 49.606 127 61 3 151 276 83 207 1.38e-35 135
MS.gene75620.t1 MTR_4g088555 55.963 109 32 1 130 238 44 136 1.59e-34 125
MS.gene75620.t1 MTR_2g078770 61.798 89 34 0 150 238 43 131 1.81e-34 126
MS.gene75620.t1 MTR_8g005960 48.684 152 63 3 118 261 147 291 5.41e-34 130
MS.gene75620.t1 MTR_8g096780 50.862 116 54 2 151 266 85 197 1.66e-32 127
MS.gene75620.t1 MTR_7g028740 63.750 80 29 0 165 244 77 156 3.66e-32 125
MS.gene75620.t1 MTR_2g014200 57.895 95 39 1 148 242 48 141 4.69e-32 119
MS.gene75620.t1 MTR_2g461920 60.000 90 36 0 151 240 157 246 8.25e-31 124
MS.gene75620.t1 MTR_1g086250 56.842 95 41 0 150 244 132 226 3.41e-29 121
MS.gene75620.t1 MTR_2g046550 61.446 83 32 0 165 247 139 221 1.75e-28 119
MS.gene75620.t1 MTR_1g035010 58.621 87 36 0 163 249 102 188 2.46e-28 118
MS.gene75620.t1 MTR_7g110320 61.250 80 31 0 165 244 145 224 1.68e-26 113
MS.gene75620.t1 MTR_7g110320 61.250 80 31 0 165 244 145 224 2.17e-26 112
MS.gene75620.t1 MTR_2g020620 52.239 67 32 0 172 238 97 163 1.76e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75620.t1 AT5G43270 40.052 382 164 16 1 353 1 346 1.46e-55 190
MS.gene75620.t1 AT5G43270 40.052 382 164 16 1 353 1 346 1.46e-55 190
MS.gene75620.t1 AT5G43270 40.052 382 164 16 1 353 1 346 1.46e-55 190
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 5.61e-53 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27370 47.287 258 111 7 1 240 1 251 2.12e-52 181
MS.gene75620.t1 AT1G27360 39.005 382 193 15 1 353 1 371 7.17e-52 179
MS.gene75620.t1 AT1G27360 39.005 382 193 15 1 353 1 371 7.17e-52 179
MS.gene75620.t1 AT1G27360 39.005 382 193 15 1 353 1 371 7.17e-52 179
MS.gene75620.t1 AT1G27360 39.005 382 193 15 1 353 1 371 7.17e-52 179
MS.gene75620.t1 AT2G42200 39.837 246 125 9 120 358 32 261 7.97e-37 139
MS.gene75620.t1 AT1G69170 66.304 92 31 0 148 239 107 198 1.46e-35 135
MS.gene75620.t1 AT3G57920 53.279 122 44 3 120 241 27 135 3.02e-34 131
MS.gene75620.t1 AT1G02065 53.279 122 49 2 118 238 147 261 7.03e-33 127
MS.gene75620.t1 AT5G50670 60.976 82 32 0 163 244 99 180 1.36e-31 124
MS.gene75620.t1 AT5G50670 60.976 82 32 0 163 244 99 180 1.36e-31 124
MS.gene75620.t1 AT5G50570 60.976 82 32 0 163 244 99 180 1.36e-31 124
MS.gene75620.t1 AT5G50570 60.976 82 32 0 163 244 99 180 1.36e-31 124
MS.gene75620.t1 AT2G33810 59.091 88 33 1 151 238 43 127 7.88e-31 115
MS.gene75620.t1 AT1G76580 60.674 89 35 0 163 251 112 200 1.75e-30 124
MS.gene75620.t1 AT1G76580 60.674 89 35 0 163 251 112 200 2.22e-30 124
MS.gene75620.t1 AT1G76580 60.674 89 35 0 163 251 112 200 3.73e-30 124
MS.gene75620.t1 AT3G60030 61.250 80 31 0 165 244 127 206 2.69e-29 121
MS.gene75620.t1 AT1G53160 44.444 117 57 1 165 273 54 170 8.77e-29 111
MS.gene75620.t1 AT2G47070 60.215 93 36 1 165 256 106 198 1.03e-28 119
MS.gene75620.t1 AT1G53160 45.385 130 56 4 165 288 54 174 1.28e-28 111
MS.gene75620.t1 AT1G53160 45.385 130 56 4 165 288 54 174 1.28e-28 111
MS.gene75620.t1 AT1G20980 60.000 95 37 1 151 244 106 200 3.05e-28 118
MS.gene75620.t1 AT3G15270 60.811 74 29 0 165 238 63 136 4.24e-28 110
MS.gene75620.t1 AT1G02065 46.875 96 43 2 118 212 147 235 6.53e-19 86.3
MS.gene75620.t1 AT5G18830 51.316 76 36 1 165 239 138 213 5.08e-18 87.0
MS.gene75620.t1 AT5G18830 51.316 76 36 1 165 239 138 213 5.19e-18 87.0
MS.gene75620.t1 AT5G18830 51.316 76 36 1 165 239 138 213 5.32e-18 87.0
MS.gene75620.t1 AT2G47070 60.294 68 26 1 190 256 2 69 2.28e-16 82.0

Find 116 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGATCAGACAAGGTATT+TGG 0.193506 3.3:-71183595 None:intergenic
GCTAAATTTGAAGTATTCTT+TGG 0.209568 3.3:-71183222 None:intergenic
TGCAGAGGAAGAGGCTTTCT+TGG 0.222637 3.3:-71182259 None:intergenic
AGCTTGCTGTTGGGCATGTC+TGG 0.241731 3.3:-71183249 None:intergenic
GGATTCAATTTCACTGCTTC+AGG 0.274502 3.3:-71182681 None:intergenic
GAGCATAAAATCTTTGGTTT+GGG 0.292731 3.3:-71183274 None:intergenic
TTTAGACGATTCTTTCTTAC+CGG 0.323854 3.3:-71182139 None:intergenic
TGAGCATAAAATCTTTGGTT+TGG 0.329729 3.3:-71183275 None:intergenic
TTAACATCTTGTGTAGCATT+TGG 0.347549 3.3:-71183613 None:intergenic
GCAAGGGAAACTTGACTCAT+AGG 0.354069 3.3:-71183875 None:intergenic
AATCTTGGTTCCTTTGCCTT+TGG 0.356006 3.3:-71183364 None:intergenic
TTGTGGCCTCGACCTCTCTT+TGG 0.373974 3.3:+71182356 MS.gene75620:CDS
CTCTTCTGTCAACCAATTCA+TGG 0.377885 3.3:+71183647 MS.gene75620:CDS
TACTACCACCACAGGCGATT+TGG 0.381836 3.3:-71182421 None:intergenic
TTCTTACCGGTGGAATTGTC+TGG 0.385894 3.3:-71182126 None:intergenic
ACATGCACCTGCTACATTGC+TGG 0.389565 3.3:-71182467 None:intergenic
AATCTCCCGGGCAATGGGAC+TGG 0.397861 3.3:+71181866 MS.gene75620:CDS
GCTGCTGGAGAAGTTTCAAT+TGG 0.402606 3.3:-71182168 None:intergenic
AGCTGCCAGGTGGAAGGTTG+TGG 0.411491 3.3:+71182339 MS.gene75620:CDS
ATCTCCCGGGCAATGGGACT+GGG 0.414643 3.3:+71181867 MS.gene75620:CDS
CAGCGCCTGTATCTCGTTCT+CGG 0.419065 3.3:+71183804 MS.gene75620:CDS
AATTTCACTGCTTCAGGCTG+AGG 0.421915 3.3:-71182675 None:intergenic
ACAATCTACTGATTGGAGTA+TGG 0.424631 3.3:+71181936 MS.gene75620:CDS
CGTGAAATCTCCCGGGCAAT+GGG 0.426658 3.3:+71181861 MS.gene75620:CDS
CAATATTCCAGTGACGAAAA+AGG 0.427818 3.3:-71183754 None:intergenic
GTGAGCAATGGTTCTGCTGC+TGG 0.432022 3.3:-71182183 None:intergenic
GTATTCTTTGGAAAAGCAAA+TGG 0.434089 3.3:-71183210 None:intergenic
ACAACCTGGGACAGCAATCT+TGG 0.439368 3.3:-71183379 None:intergenic
AAGTTTCCCTTGCAACCAGC+TGG 0.440887 3.3:+71183885 MS.gene75620:CDS
ATACACTCCAATCGGACAAC+CGG 0.451604 3.3:+71183700 MS.gene75620:CDS
TACTTTGAGGATGTTAGCAC+TGG 0.451656 3.3:+71182228 MS.gene75620:CDS
TCTTCTGTCAACCAATTCAT+GGG 0.452114 3.3:+71183648 MS.gene75620:CDS
CGAGATACAGGCGCTGGAGC+TGG 0.455430 3.3:-71183797 None:intergenic
CATCAAACTCTGAGAGATCA+TGG 0.461380 3.3:-71182596 None:intergenic
CAACCTTCCACCTGGCAGCT+TGG 0.467365 3.3:-71182336 None:intergenic
TCGCCTGTGGTGGTAGTAGC+TGG 0.470263 3.3:+71182426 MS.gene75620:CDS
GTTGGGATGTTGATTCCATC+TGG 0.471449 3.3:+71181979 MS.gene75620:CDS
CCTTTCTTTCCGCACAAAAG+AGG 0.471950 3.3:-71182285 None:intergenic
ACAGGTTACAATCTACTGAT+TGG 0.476801 3.3:+71181929 MS.gene75620:CDS
TGGTAAAAGATTCTACTTTG+AGG 0.477123 3.3:+71182215 MS.gene75620:CDS
AGGGGTGCCGGTTGTCCGAT+TGG 0.477616 3.3:-71183707 None:intergenic
TGTGGTGGTAGTAGCTGGTT+TGG 0.481417 3.3:+71182431 MS.gene75620:CDS
ACGTGAAATCTCCCGGGCAA+TGG 0.485685 3.3:+71181860 MS.gene75620:CDS
GAAGATCGAGAAATCAATGT+TGG 0.486543 3.3:+71181961 MS.gene75620:CDS
AAGAGGAACTGCAGAGGAAG+AGG 0.487764 3.3:-71182268 None:intergenic
ATCATATGGACCCCATGAAT+TGG 0.492799 3.3:-71183659 None:intergenic
TTGTCCGATTGGAGTGTATG+AGG 0.494492 3.3:-71183696 None:intergenic
AGCATACTCATTAATCCAGC+TGG 0.497222 3.3:-71183900 None:intergenic
GATTGGAGTGTATGAGGGAG+GGG 0.501793 3.3:-71183690 None:intergenic
CTTTGCCTTTGGATATAAAG+TGG 0.501899 3.3:-71183353 None:intergenic
GAGTGGAACGTGAAATCTCC+CGG 0.503280 3.3:+71181853 MS.gene75620:CDS
CATTAATCCAGCTGGTTGCA+AGG 0.507260 3.3:-71183892 None:intergenic
TTGATTCCATCTGGTGGTAG+TGG 0.508737 3.3:+71181988 MS.gene75620:CDS
AAGATCGAGAAATCAATGTT+GGG 0.508795 3.3:+71181962 MS.gene75620:CDS
CACAAATTCTATGTTTCCGA+TGG 0.510237 3.3:-71182389 None:intergenic
CAAGGGAAACTTGACTCATA+GGG 0.517421 3.3:-71183874 None:intergenic
CGTGCTAAATCCAAGCTGCC+AGG 0.518784 3.3:+71182326 MS.gene75620:CDS
GTAACCCGAGAACGAGATAC+AGG 0.528459 3.3:-71183809 None:intergenic
CGATTGGAGTGTATGAGGGA+GGG 0.531040 3.3:-71183691 None:intergenic
TGCAGATGGGAGGCAGACAA+TGG 0.534432 3.3:+71183185 MS.gene75620:intron
CCTCCCTCATACACTCCAAT+CGG 0.535964 3.3:+71183692 MS.gene75620:CDS
TATTCTTTGGAAAAGCAAAT+GGG 0.538218 3.3:-71183209 None:intergenic
AGCGCCTGTATCTCGTTCTC+GGG 0.540985 3.3:+71183805 MS.gene75620:CDS
ACATCAATGTTAACTCAAGA+AGG 0.548574 3.3:+71182099 MS.gene75620:CDS
GGAACCAAGATTGCTGTCCC+AGG 0.548599 3.3:+71183375 MS.gene75620:CDS
GCAGATGGGAGGCAGACAAT+GGG 0.549297 3.3:+71183186 MS.gene75620:intron
CAAAAGCAGCAGAAACTCAC+AGG 0.550624 3.3:+71181911 MS.gene75620:CDS
GGTAAGAAAGAATCGTCTAA+AGG 0.551763 3.3:+71182141 MS.gene75620:CDS
TTGGCACACTGATCAACATC+AGG 0.552387 3.3:+71183774 MS.gene75620:CDS
TTTGGATATAAAGTGGCCAC+TGG 0.552682 3.3:-71183346 None:intergenic
AGTTGCATACTGAGGGGTGC+CGG 0.554723 3.3:-71183719 None:intergenic
CCAAGCTTTAGTGTGAGCAA+TGG 0.557830 3.3:-71182195 None:intergenic
GGGGACGAAGAGAGAGCTGA+TGG 0.559258 3.3:-71182702 None:intergenic
ATTGGAGTGTATGAGGGAGG+GGG 0.561383 3.3:-71183689 None:intergenic
AGAATACTTCAAATTTAGCA+TGG 0.561391 3.3:+71183226 MS.gene75620:CDS
GAATAATTCATCATCAAATG+AGG 0.562543 3.3:+71182068 MS.gene75620:CDS
ATTAATCCAGCTGGTTGCAA+GGG 0.568989 3.3:-71183891 None:intergenic
CGCTGAGCAGTTGCATACTG+AGG 0.570454 3.3:-71183727 None:intergenic
AATCCAAGCTGCCAGGTGGA+AGG 0.571632 3.3:+71182333 MS.gene75620:CDS
CTTTAGCCAAAGAGAGGTCG+AGG 0.573405 3.3:-71182362 None:intergenic
CGAGGCCACAACCTTCCACC+TGG 0.580184 3.3:-71182344 None:intergenic
CTTGAGATTTATACCATAGG+GGG 0.580458 3.3:-71182721 None:intergenic
AGACGATTCTTTCTTACCGG+TGG 0.582162 3.3:-71182136 None:intergenic
AGTGGCCACTTTATATCCAA+AGG 0.587042 3.3:+71183348 MS.gene75620:CDS
CCATTGCTCACACTAAAGCT+TGG 0.588106 3.3:+71182195 MS.gene75620:CDS
CTTTGGCTAAAGATTACCAT+CGG 0.588534 3.3:+71182373 MS.gene75620:CDS
TGTCCGATTGGAGTGTATGA+GGG 0.588850 3.3:-71183695 None:intergenic
GTCTTTCAGATCACAATGCA+AGG 0.591028 3.3:+71182643 MS.gene75620:CDS
GCATAAAATCTTTGGTTTGG+GGG 0.594228 3.3:-71183272 None:intergenic
GCTGAGCAGTTGCATACTGA+GGG 0.602329 3.3:-71183726 None:intergenic
TTGACAGAAGAGAGAGAGCA+CGG 0.603893 3.3:-71183636 None:intergenic
AGCATAAAATCTTTGGTTTG+GGG 0.604215 3.3:-71183273 None:intergenic
CCGATTGGAGTGTATGAGGG+AGG 0.604444 3.3:-71183692 None:intergenic
CACTTTATATCCAAAGGCAA+AGG 0.613225 3.3:+71183354 MS.gene75620:CDS
CGAGAACGAGATACAGGCGC+TGG 0.614056 3.3:-71183803 None:intergenic
AAACCAGCTACTACCACCAC+AGG 0.617364 3.3:-71182429 None:intergenic
TTGCATTGTGATCTGAAAGA+CGG 0.621589 3.3:-71182641 None:intergenic
TGTGAGGACTTGTCATGGAG+TGG 0.623994 3.3:+71181836 None:intergenic
GAATAGCCACTACCACCAGA+TGG 0.631021 3.3:-71181994 None:intergenic
TCATTCCAAATCGCCTGTGG+TGG 0.632811 3.3:+71182416 MS.gene75620:CDS
CTTCTGTCAACCAATTCATG+GGG 0.634971 3.3:+71183649 MS.gene75620:CDS
AGTGGAACGTGAAATCTCCC+GGG 0.641060 3.3:+71181854 MS.gene75620:CDS
ATCTACTGATTGGAGTATGG+AGG 0.643141 3.3:+71181939 MS.gene75620:CDS
TTGGAGTGTATGAGGGAGGG+GGG 0.645515 3.3:-71183688 None:intergenic
GGGATGTTGATTCCATCTGG+TGG 0.647072 3.3:+71181982 MS.gene75620:CDS
CAGTCATTCCAAATCGCCTG+TGG 0.648152 3.3:+71182413 MS.gene75620:CDS
TGTAGCATTTGGATCAGACA+AGG 0.648361 3.3:-71183602 None:intergenic
ATATTCATTCTGCAGATGGG+AGG 0.649420 3.3:+71183175 MS.gene75620:intron
GGTTCTCCAGACAATTCCAC+CGG 0.652614 3.3:+71182120 MS.gene75620:CDS
TCTTGAGATTTATACCATAG+GGG 0.653113 3.3:-71182722 None:intergenic
GGTAATCTTTAGCCAAAGAG+AGG 0.662641 3.3:-71182368 None:intergenic
CTGAGCAGTTGCATACTGAG+GGG 0.662729 3.3:-71183725 None:intergenic
GCTAAATCCAAGCTGCCAGG+TGG 0.663879 3.3:+71182329 MS.gene75620:CDS
ATGCTATCTGATGATTCCAG+TGG 0.666446 3.3:+71183330 MS.gene75620:CDS
TAATGCATGCTTCATCCTCG+AGG 0.674783 3.3:+71182028 MS.gene75620:CDS
GCACAAAAGAGGAACTGCAG+AGG 0.694164 3.3:-71182274 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACACTTAATATTTTCTTATA+AGG - chr3.3:71183063-71183082 None:intergenic 15.0%
!!! ATTAAATTTAGTTTGTTCAA+TGG + chr3.3:71183443-71183462 MS.gene75620:intron 15.0%
!!! TTGGTTTGTTTAAAATAAAT+TGG + chr3.3:71182537-71182556 MS.gene75620:intron 15.0%
!! ATTGATAGAATACATATTAC+AGG + chr3.3:71183562-71183581 MS.gene75620:intron 20.0%
!! TTATGAGAGAATTATAAGAA+TGG + chr3.3:71182843-71182862 MS.gene75620:intron 20.0%
!! TTTAATAAGAAATCATGACT+CGG - chr3.3:71183429-71183448 None:intergenic 20.0%
!!! AACTTAAACTATGATTTTGA+AGG + chr3.3:71182571-71182590 MS.gene75620:intron 20.0%
!!! ATACTCTATGACTATATTTT+TGG - chr3.3:71182990-71183009 None:intergenic 20.0%
!!! TACTCTATGACTATATTTTT+GGG - chr3.3:71182989-71183008 None:intergenic 20.0%
!!! TTAGAATTTCTTTTGAGAAA+TGG - chr3.3:71182883-71182902 None:intergenic 20.0%
! AGAATACTTCAAATTTAGCA+TGG + chr3.3:71183226-71183245 MS.gene75620:CDS 25.0%
! ATTCTTGAGATTTATACCAT+AGG - chr3.3:71182727-71182746 None:intergenic 25.0%
! GAATAATTCATCATCAAATG+AGG + chr3.3:71182068-71182087 MS.gene75620:CDS 25.0%
! TTAATATTCATTCTGCAGAT+GGG + chr3.3:71183172-71183191 MS.gene75620:intron 25.0%
! TTCAGTTTCTTTGTAAGAAA+TGG + chr3.3:71183009-71183028 MS.gene75620:intron 25.0%
! TTCTTGAGATTTATACCATA+GGG - chr3.3:71182726-71182745 None:intergenic 25.0%
! TTTAAACAAACCAAAAAGAC+AGG - chr3.3:71182531-71182550 None:intergenic 25.0%
! TTTAATATTCATTCTGCAGA+TGG + chr3.3:71183171-71183190 MS.gene75620:intron 25.0%
!! GCTAAATTTGAAGTATTCTT+TGG - chr3.3:71183225-71183244 None:intergenic 25.0%
!! TATTCTTTGGAAAAGCAAAT+GGG - chr3.3:71183212-71183231 None:intergenic 25.0%
!!! AGTTTTGAGCATAAAATCTT+TGG - chr3.3:71183283-71183302 None:intergenic 25.0%
!!! TAACAGTTGTTTCTACAAAT+AGG - chr3.3:71183537-71183556 None:intergenic 25.0%
AAGATCGAGAAATCAATGTT+GGG + chr3.3:71181962-71181981 MS.gene75620:CDS 30.0%
ACATCAATGTTAACTCAAGA+AGG + chr3.3:71182099-71182118 MS.gene75620:CDS 30.0%
TAAACTAATGAAGCAAGACA+AGG - chr3.3:71182773-71182792 None:intergenic 30.0%
TACAACCAATAGCATATCAA+TGG - chr3.3:71182909-71182928 None:intergenic 30.0%
TCTTGAGATTTATACCATAG+GGG - chr3.3:71182725-71182744 None:intergenic 30.0%
TGAAACATGCAATAAACTAG+AGG - chr3.3:71183495-71183514 None:intergenic 30.0%
TGAATGGCGATATCTTAAAA+AGG + chr3.3:71182820-71182839 MS.gene75620:intron 30.0%
TGAGCATAAAATCTTTGGTT+TGG - chr3.3:71183278-71183297 None:intergenic 30.0%
TGGTAAAAGATTCTACTTTG+AGG + chr3.3:71182215-71182234 MS.gene75620:CDS 30.0%
TTAACATCTTGTGTAGCATT+TGG - chr3.3:71183616-71183635 None:intergenic 30.0%
TTAAGATTAGAGAACAACCT+GGG - chr3.3:71183395-71183414 None:intergenic 30.0%
TTTAGACGATTCTTTCTTAC+CGG - chr3.3:71182142-71182161 None:intergenic 30.0%
! AAGGCTAATTGATCTTTTAG+GGG - chr3.3:71182754-71182773 None:intergenic 30.0%
! ACAAGGCTAATTGATCTTTT+AGG - chr3.3:71182756-71182775 None:intergenic 30.0%
! ATATGCTATTGGTTGTATGA+AGG + chr3.3:71182912-71182931 MS.gene75620:intron 30.0%
! ATTGTCAATGTCATTTGCAT+TGG - chr3.3:71182502-71182521 None:intergenic 30.0%
! CAAGGCTAATTGATCTTTTA+GGG - chr3.3:71182755-71182774 None:intergenic 30.0%
! GAGCATAAAATCTTTGGTTT+GGG - chr3.3:71183277-71183296 None:intergenic 30.0%
! GTATTCTTTGGAAAAGCAAA+TGG - chr3.3:71183213-71183232 None:intergenic 30.0%
! TTTAAGATTAGAGAACAACC+TGG - chr3.3:71183396-71183415 None:intergenic 30.0%
!! AGCATAAAATCTTTGGTTTG+GGG - chr3.3:71183276-71183295 None:intergenic 30.0%
!!! ACAAACTTGCTGAAGTTTTT+TGG - chr3.3:71183306-71183325 None:intergenic 30.0%
AACCAATAGCATATCAATGG+AGG - chr3.3:71182906-71182925 None:intergenic 35.0%
AACCTCCATTGATATGCTAT+TGG + chr3.3:71182901-71182920 MS.gene75620:intron 35.0%
ACAGGTTACAATCTACTGAT+TGG + chr3.3:71181929-71181948 MS.gene75620:CDS 35.0%
ATCCCAAACGAGAAACAAAA+TGG - chr3.3:71182801-71182820 None:intergenic 35.0%
CAATATTCCAGTGACGAAAA+AGG - chr3.3:71183757-71183776 None:intergenic 35.0%
CACAAATTCTATGTTTCCGA+TGG - chr3.3:71182392-71182411 None:intergenic 35.0%
CACTTTATATCCAAAGGCAA+AGG + chr3.3:71183354-71183373 MS.gene75620:CDS 35.0%
CTTGAGATTTATACCATAGG+GGG - chr3.3:71182724-71182743 None:intergenic 35.0%
CTTTGCCTTTGGATATAAAG+TGG - chr3.3:71183356-71183375 None:intergenic 35.0%
CTTTGGCTAAAGATTACCAT+CGG + chr3.3:71182373-71182392 MS.gene75620:CDS 35.0%
GAAGATCGAGAAATCAATGT+TGG + chr3.3:71181961-71181980 MS.gene75620:CDS 35.0%
GGTAAGAAAGAATCGTCTAA+AGG + chr3.3:71182141-71182160 MS.gene75620:CDS 35.0%
TCTTCTGTCAACCAATTCAT+GGG + chr3.3:71183648-71183667 MS.gene75620:CDS 35.0%
TTGCATTGTGATCTGAAAGA+CGG - chr3.3:71182644-71182663 None:intergenic 35.0%
! AATAAGAAATCATGACTCGG+AGG - chr3.3:71183426-71183445 None:intergenic 35.0%
! ACAATCTACTGATTGGAGTA+TGG + chr3.3:71181936-71181955 MS.gene75620:CDS 35.0%
! TTTGGATCAGACAAGGTATT+TGG - chr3.3:71183598-71183617 None:intergenic 35.0%
!! CAATTAGCTTCCTGTCTTTT+TGG + chr3.3:71182518-71182537 MS.gene75620:intron 35.0%
!! CAGTTGTTTCTACAAATAGG+AGG - chr3.3:71183534-71183553 None:intergenic 35.0%
!! CTAATTGATCTTTTAGGGGA+GGG - chr3.3:71182750-71182769 None:intergenic 35.0%
!! CTTTTTCGTCACTGGAATAT+TGG + chr3.3:71183755-71183774 MS.gene75620:CDS 35.0%
!! GCATAAAATCTTTGGTTTGG+GGG - chr3.3:71183275-71183294 None:intergenic 35.0%
!!! TGCCATTTTGTTTCTCGTTT+GGG + chr3.3:71182796-71182815 MS.gene75620:intron 35.0%
!!! TTGCCATTTTGTTTCTCGTT+TGG + chr3.3:71182795-71182814 MS.gene75620:intron 35.0%
AATCTTGGTTCCTTTGCCTT+TGG - chr3.3:71183367-71183386 None:intergenic 40.0%
ATATTCATTCTGCAGATGGG+AGG + chr3.3:71183175-71183194 MS.gene75620:intron 40.0%
ATCATATGGACCCCATGAAT+TGG - chr3.3:71183662-71183681 None:intergenic 40.0%
ATGCTATCTGATGATTCCAG+TGG + chr3.3:71183330-71183349 MS.gene75620:CDS 40.0%
CAAGGGAAACTTGACTCATA+GGG - chr3.3:71183877-71183896 None:intergenic 40.0%
CTCTTCTGTCAACCAATTCA+TGG + chr3.3:71183647-71183666 MS.gene75620:CDS 40.0%
CTTCTGTCAACCAATTCATG+GGG + chr3.3:71183649-71183668 MS.gene75620:CDS 40.0%
GGATTCAATTTCACTGCTTC+AGG - chr3.3:71182684-71182703 None:intergenic 40.0%
GGTAATCTTTAGCCAAAGAG+AGG - chr3.3:71182371-71182390 None:intergenic 40.0%
GTCTTTCAGATCACAATGCA+AGG + chr3.3:71182643-71182662 MS.gene75620:CDS 40.0%
TGTAGCATTTGGATCAGACA+AGG - chr3.3:71183605-71183624 None:intergenic 40.0%
TTTGGATATAAAGTGGCCAC+TGG - chr3.3:71183349-71183368 None:intergenic 40.0%
! AGTGGCCACTTTATATCCAA+AGG + chr3.3:71183348-71183367 MS.gene75620:CDS 40.0%
! ATCTACTGATTGGAGTATGG+AGG + chr3.3:71181939-71181958 MS.gene75620:CDS 40.0%
! CATCAAACTCTGAGAGATCA+TGG - chr3.3:71182599-71182618 None:intergenic 40.0%
! TACTTTGAGGATGTTAGCAC+TGG + chr3.3:71182228-71182247 MS.gene75620:CDS 40.0%
! TGTTTCTCGTTTGGGATGAA+TGG + chr3.3:71182804-71182823 MS.gene75620:intron 40.0%
!! GCTAATTGATCTTTTAGGGG+AGG - chr3.3:71182751-71182770 None:intergenic 40.0%
AATTTCACTGCTTCAGGCTG+AGG - chr3.3:71182678-71182697 None:intergenic 45.0%
AGACGATTCTTTCTTACCGG+TGG - chr3.3:71182139-71182158 None:intergenic 45.0%
ATACACTCCAATCGGACAAC+CGG + chr3.3:71183700-71183719 MS.gene75620:CDS 45.0%
CAAAAGCAGCAGAAACTCAC+AGG + chr3.3:71181911-71181930 MS.gene75620:CDS 45.0%
CCATTGCTCACACTAAAGCT+TGG + chr3.3:71182195-71182214 MS.gene75620:CDS 45.0%
CCTTTCTTTCCGCACAAAAG+AGG - chr3.3:71182288-71182307 None:intergenic 45.0%
GCAAGGGAAACTTGACTCAT+AGG - chr3.3:71183878-71183897 None:intergenic 45.0%
GCTGCTGGAGAAGTTTCAAT+TGG - chr3.3:71182171-71182190 None:intergenic 45.0%
TAATGCATGCTTCATCCTCG+AGG + chr3.3:71182028-71182047 MS.gene75620:CDS 45.0%
TGTCCGATTGGAGTGTATGA+GGG - chr3.3:71183698-71183717 None:intergenic 45.0%
TTCTTACCGGTGGAATTGTC+TGG - chr3.3:71182129-71182148 None:intergenic 45.0%
TTGACAGAAGAGAGAGAGCA+CGG - chr3.3:71183639-71183658 None:intergenic 45.0%
TTGATTCCATCTGGTGGTAG+TGG + chr3.3:71181988-71182007 MS.gene75620:CDS 45.0%
TTGGCACACTGATCAACATC+AGG + chr3.3:71183774-71183793 MS.gene75620:CDS 45.0%
TTGTCCGATTGGAGTGTATG+AGG - chr3.3:71183699-71183718 None:intergenic 45.0%
! CCAAGCTTTAGTGTGAGCAA+TGG - chr3.3:71182198-71182217 None:intergenic 45.0%
! CCTCTTTTGTGCGGAAAGAA+AGG + chr3.3:71182285-71182304 MS.gene75620:CDS 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr3.3:71182460-71182479 MS.gene75620:CDS 45.0%
! TCTGCAGTTCCTCTTTTGTG+CGG + chr3.3:71182276-71182295 MS.gene75620:CDS 45.0%
!! GTTGGGATGTTGATTCCATC+TGG + chr3.3:71181979-71181998 MS.gene75620:CDS 45.0%
AAACCAGCTACTACCACCAC+AGG - chr3.3:71182432-71182451 None:intergenic 50.0%
AAGAGGAACTGCAGAGGAAG+AGG - chr3.3:71182271-71182290 None:intergenic 50.0%
AAGTTTCCCTTGCAACCAGC+TGG + chr3.3:71183885-71183904 MS.gene75620:CDS 50.0%
ACAACCTGGGACAGCAATCT+TGG - chr3.3:71183382-71183401 None:intergenic 50.0%
ACATGCACCTGCTACATTGC+TGG - chr3.3:71182470-71182489 None:intergenic 50.0%
AGTGGAACGTGAAATCTCCC+GGG + chr3.3:71181854-71181873 MS.gene75620:CDS 50.0%
ATTGGAGTGTATGAGGGAGG+GGG - chr3.3:71183692-71183711 None:intergenic 50.0%
CAGTCATTCCAAATCGCCTG+TGG + chr3.3:71182413-71182432 MS.gene75620:CDS 50.0%
CCTCCCTCATACACTCCAAT+CGG + chr3.3:71183692-71183711 MS.gene75620:CDS 50.0%
CGATTGGAGTGTATGAGGGA+GGG - chr3.3:71183694-71183713 None:intergenic 50.0%
CTGAGCAGTTGCATACTGAG+GGG - chr3.3:71183728-71183747 None:intergenic 50.0%
CTTTAGCCAAAGAGAGGTCG+AGG - chr3.3:71182365-71182384 None:intergenic 50.0%
GAATAGCCACTACCACCAGA+TGG - chr3.3:71181997-71182016 None:intergenic 50.0%
GAGTGGAACGTGAAATCTCC+CGG + chr3.3:71181853-71181872 MS.gene75620:CDS 50.0%
GATTGGAGTGTATGAGGGAG+GGG - chr3.3:71183693-71183712 None:intergenic 50.0%
GCACAAAAGAGGAACTGCAG+AGG - chr3.3:71182277-71182296 None:intergenic 50.0%
GCTGAGCAGTTGCATACTGA+GGG - chr3.3:71183729-71183748 None:intergenic 50.0%
GGTTCTCCAGACAATTCCAC+CGG + chr3.3:71182120-71182139 MS.gene75620:CDS 50.0%
GTAACCCGAGAACGAGATAC+AGG - chr3.3:71183812-71183831 None:intergenic 50.0%
TACTACCACCACAGGCGATT+TGG - chr3.3:71182424-71182443 None:intergenic 50.0%
TCATTCCAAATCGCCTGTGG+TGG + chr3.3:71182416-71182435 MS.gene75620:CDS 50.0%
TGCAGAGGAAGAGGCTTTCT+TGG - chr3.3:71182262-71182281 None:intergenic 50.0%
TGTGGTGGTAGTAGCTGGTT+TGG + chr3.3:71182431-71182450 MS.gene75620:CDS 50.0%
! AGCAGATTTTGAGCTCCTCG+AGG - chr3.3:71182046-71182065 None:intergenic 50.0%
!! GGGATGTTGATTCCATCTGG+TGG + chr3.3:71181982-71182001 MS.gene75620:CDS 50.0%
AATCCAAGCTGCCAGGTGGA+AGG + chr3.3:71182333-71182352 MS.gene75620:CDS 55.0%
ACGTGAAATCTCCCGGGCAA+TGG + chr3.3:71181860-71181879 MS.gene75620:CDS 55.0%
AGCGCCTGTATCTCGTTCTC+GGG + chr3.3:71183805-71183824 MS.gene75620:CDS 55.0%
CAGCGCCTGTATCTCGTTCT+CGG + chr3.3:71183804-71183823 MS.gene75620:CDS 55.0%
CCGATTGGAGTGTATGAGGG+AGG - chr3.3:71183695-71183714 None:intergenic 55.0%
CGCTGAGCAGTTGCATACTG+AGG - chr3.3:71183730-71183749 None:intergenic 55.0%
CGTGAAATCTCCCGGGCAAT+GGG + chr3.3:71181861-71181880 MS.gene75620:CDS 55.0%
CGTGCTAAATCCAAGCTGCC+AGG + chr3.3:71182326-71182345 MS.gene75620:CDS 55.0%
GCAGATGGGAGGCAGACAAT+GGG + chr3.3:71183186-71183205 MS.gene75620:intron 55.0%
GCTAAATCCAAGCTGCCAGG+TGG + chr3.3:71182329-71182348 MS.gene75620:CDS 55.0%
GGAACCAAGATTGCTGTCCC+AGG + chr3.3:71183375-71183394 MS.gene75620:CDS 55.0%
TGCAGATGGGAGGCAGACAA+TGG + chr3.3:71183185-71183204 MS.gene75620:intron 55.0%
TTGGAGTGTATGAGGGAGGG+GGG - chr3.3:71183691-71183710 None:intergenic 55.0%
TTGTGGCCTCGACCTCTCTT+TGG + chr3.3:71182356-71182375 MS.gene75620:CDS 55.0%
! AGTTGCATACTGAGGGGTGC+CGG - chr3.3:71183722-71183741 None:intergenic 55.0%
! GAGGGGGGCTTGGTATCATA+TGG - chr3.3:71183676-71183695 None:intergenic 55.0%
! GTGAGCAATGGTTCTGCTGC+TGG - chr3.3:71182186-71182205 None:intergenic 55.0%
! GTTTTCCCAGTCCCATTGCC+CGG - chr3.3:71181875-71181894 None:intergenic 55.0%
! TTTTCCCAGTCCCATTGCCC+GGG - chr3.3:71181874-71181893 None:intergenic 55.0%
!! AGCTTGCTGTTGGGCATGTC+TGG - chr3.3:71183252-71183271 None:intergenic 55.0%
!! GCGCTCACCTTTTTCGTCAC+TGG + chr3.3:71183747-71183766 MS.gene75620:CDS 55.0%
!! GTTTGGGGGAGCTTGCTGTT+GGG - chr3.3:71183261-71183280 None:intergenic 55.0%
AATCTCCCGGGCAATGGGAC+TGG + chr3.3:71181866-71181885 MS.gene75620:CDS 60.0%
AGCTGCCAGGTGGAAGGTTG+TGG + chr3.3:71182339-71182358 MS.gene75620:CDS 60.0%
ATCTCCCGGGCAATGGGACT+GGG + chr3.3:71181867-71181886 MS.gene75620:CDS 60.0%
CAACCTTCCACCTGGCAGCT+TGG - chr3.3:71182339-71182358 None:intergenic 60.0%
CGAGAACGAGATACAGGCGC+TGG - chr3.3:71183806-71183825 None:intergenic 60.0%
GCTCTCTCTTCGTCCCCCTA+TGG + chr3.3:71182708-71182727 MS.gene75620:CDS 60.0%
GGGGACGAAGAGAGAGCTGA+TGG - chr3.3:71182705-71182724 None:intergenic 60.0%
TCGCCTGTGGTGGTAGTAGC+TGG + chr3.3:71182426-71182445 MS.gene75620:CDS 60.0%
!! GGTTTGGGGGAGCTTGCTGT+TGG - chr3.3:71183262-71183281 None:intergenic 60.0%
CGAGGCCACAACCTTCCACC+TGG - chr3.3:71182347-71182366 None:intergenic 65.0%
GTGTATGAGGGAGGGGGGCT+TGG - chr3.3:71183686-71183705 None:intergenic 65.0%
!! AGGGGTGCCGGTTGTCCGAT+TGG - chr3.3:71183710-71183729 None:intergenic 65.0%
!! CGAGATACAGGCGCTGGAGC+TGG - chr3.3:71183800-71183819 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 71181850 71183912 71181850 ID=MS.gene75620
chr3.3 mRNA 71181850 71183912 71181850 ID=MS.gene75620.t1;Parent=MS.gene75620
chr3.3 exon 71181850 71182481 71181850 ID=MS.gene75620.t1.exon1;Parent=MS.gene75620.t1
chr3.3 CDS 71181850 71182481 71181850 ID=cds.MS.gene75620.t1;Parent=MS.gene75620.t1
chr3.3 exon 71182593 71182729 71182593 ID=MS.gene75620.t1.exon2;Parent=MS.gene75620.t1
chr3.3 CDS 71182593 71182729 71182593 ID=cds.MS.gene75620.t1;Parent=MS.gene75620.t1
chr3.3 exon 71183190 71183396 71183190 ID=MS.gene75620.t1.exon3;Parent=MS.gene75620.t1
chr3.3 CDS 71183190 71183396 71183190 ID=cds.MS.gene75620.t1;Parent=MS.gene75620.t1
chr3.3 exon 71183584 71183912 71183584 ID=MS.gene75620.t1.exon4;Parent=MS.gene75620.t1
chr3.3 CDS 71183584 71183912 71183584 ID=cds.MS.gene75620.t1;Parent=MS.gene75620.t1
Gene Sequence

>MS.gene75620

ATGGAGTGGAACGTGAAATCTCCCGGGCAATGGGACTGGGAAAACTTATTCTTCTCGAATTCAAAAGCAGCAGAAACTCACAGGTTACAATCTACTGATTGGAGTATGGAGGAAGATCGAGAAATCAATGTTGGGATGTTGATTCCATCTGGTGGTAGTGGCTATTCAGTGTCTAAACTAATGCATGCTTCATCCTCGAGGAGCTCAAAATCTGCTTCGAATAATTCATCATCAAATGAGGACAGCAAGACATCAATGTTAACTCAAGAAGGTTCTCCAGACAATTCCACCGGTAAGAAAGAATCGTCTAAAGGAGATCCAATTGAAACTTCTCCAGCAGCAGAACCATTGCTCACACTAAAGCTTGGTAAAAGATTCTACTTTGAGGATGTTAGCACTGGAAGTCATTCCAAGAAAGCCTCTTCCTCTGCAGTTCCTCTTTTGTGCGGAAAGAAAGGTAAATCGAGCAGTCAGAACGTGCTAAATCCAAGCTGCCAGGTGGAAGGTTGTGGCCTCGACCTCTCTTTGGCTAAAGATTACCATCGGAAACATAGAATTTGTGACAGTCATTCCAAATCGCCTGTGGTGGTAGTAGCTGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTGCATGTGATTTTTTTCCAATGCAAATGACATTGACAATTAGCTTCCTGTCTTTTTGGTTTGTTTAAAATAAATTGGTTAACGATTTTAACTTAAACTATGATTTTGAAGGTTCCATGATCTCTCAGAGTTTGATGATAAAAAAAGAAGCTGCAGACGCCGTCTTTCAGATCACAATGCAAGGCGTCGCAAACCTCAGCCTGAAGCAGTGAAATTGAATCCATCAGCTCTCTCTTCGTCCCCCTATGGTATAAATCTCAAGAATCCCTCCCCTAAAAGATCAATTAGCCTTGTCTTGCTTCATTAGTTTAATTTGCCATTTTGTTTCTCGTTTGGGATGAATGGCGATATCTTAAAAAGGTTATGAGAGAATTATAAGAATGGAGCAATGATGTTCTCCATTTCTCAAAAGAAATTCTAACCTCCATTGATATGCTATTGGTTGTATGAAGGCATAATTAATGTCATCAGTTATAGAAACATGTGCTTTATATAAATTATACACCCAAAAATATAGTCATAGAGTATTCAGTTTCTTTGTAAGAAATGGAGAGATTCTCTAGTCATAAGAATAGACTCCTTATAAGAAAATATTAAGTGTTCTGATAATGTAAATCTTTGATATATGATTATGATGACACTAAAAATCTACATCATGATGATAGAATATTACATAGCAATCTATCTAATTTAATATTCATTCTGCAGATGGGAGGCAGACAATGGGCCCATTTGCTTTTCCAAAGAATACTTCAAATTTAGCATGGCCAGACATGCCCAACAGCAAGCTCCCCCAAACCAAAGATTTTATGCTCAAAACTCCAAAAAACTTCAGCAAGTTTGTCACTATGCTATCTGATGATTCCAGTGGCCACTTTATATCCAAAGGCAAAGGAACCAAGATTGCTGTCCCAGGTTGTTCTCTAATCTTAAACATTGAGCCTCCGAGTCATGATTTCTTATTAAATTTAGTTTGTTCAATGGTTCATTTAATGATCATAATTGTTTTACCTCTAGTTTATTGCATGTTTCATGAATAATAATTTTTACCTCCTATTTGTAGAAACAACTGTTATTCACATTGATAGAATACATATTACAGGTCTAGAAGATCCAAATACCTTGTCTGATCCAAATGCTACACAAGATGTTAACCGTGCTCTCTCTCTTCTGTCAACCAATTCATGGGGTCCATATGATACCAAGCCCCCCTCCCTCATACACTCCAATCGGACAACCGGCACCCCTCAGTATGCAACTGCTCAGCGCTCACCTTTTTCGTCACTGGAATATTGGCACACTGATCAACATCAGGCCAGCTCCAGCGCCTGTATCTCGTTCTCGGGTTACGACAATAGCAATCGCTTTCAAGACTTTCAGCTGTTCAGCGAACCCTATGAGTCAAGTTTCCCTTGCAACCAGCTGGATTAA
Protein sequence

>MS.gene75620.t1

MEWNVKSPGQWDWENLFFSNSKAAETHRLQSTDWSMEEDREINVGMLIPSGGSGYSVSKLMHASSSRSSKSASNNSSSNEDSKTSMLTQEGSPDNSTGKKESSKGDPIETSPAAEPLLTLKLGKRFYFEDVSTGSHSKKASSSAVPLLCGKKGKSSSQNVLNPSCQVEGCGLDLSLAKDYHRKHRICDSHSKSPVVVVAGLERRFCQQCSRFHDLSEFDDKKRSCRRRLSDHNARRRKPQPEAVKLNPSALSSSPYDGRQTMGPFAFPKNTSNLAWPDMPNSKLPQTKDFMLKTPKNFSKFVTMLSDDSSGHFISKGKGTKIAVPGLEDPNTLSDPNATQDVNRALSLLSTNSWGPYDTKPPSLIHSNRTTGTPQYATAQRSPFSSLEYWHTDQHQASSSACISFSGYDNSNRFQDFQLFSEPYESSFPCNQLD