Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75650.t1 | XP_024633575.1 | 77.5 | 258 | 44 | 3 | 21 | 266 | 1 | 256 | 4.00E-85 | 324.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75650.t1 | A0A396IXT0 | 73.8 | 256 | 20 | 4 | 21 | 263 | 1 | 222 | 1.1e-68 | 269.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene75650.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75650.t1 | MTR_3g084720 | 75.000 | 256 | 24 | 4 | 21 | 263 | 1 | 229 | 3.33e-100 | 292 |
| MS.gene75650.t1 | MTR_7g010470 | 57.500 | 80 | 33 | 1 | 188 | 266 | 1 | 80 | 9.13e-22 | 87.0 |
| MS.gene75650.t1 | MTR_6g055180 | 77.551 | 49 | 9 | 1 | 21 | 67 | 1 | 49 | 3.48e-16 | 72.4 |
| MS.gene75650.t1 | MTR_6g465230 | 44.444 | 153 | 68 | 8 | 4 | 153 | 79 | 217 | 1.20e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75650.t1 | AT2G18490 | 55.714 | 70 | 31 | 0 | 142 | 211 | 142 | 211 | 3.81e-20 | 87.0 |
| MS.gene75650.t1 | AT2G18490 | 55.714 | 70 | 31 | 0 | 142 | 211 | 192 | 261 | 5.12e-20 | 87.8 |
| MS.gene75650.t1 | AT2G15740 | 51.899 | 79 | 34 | 1 | 142 | 220 | 217 | 291 | 3.82e-19 | 85.9 |
| MS.gene75650.t1 | AT5G22990 | 50.602 | 83 | 40 | 1 | 155 | 236 | 232 | 314 | 2.50e-18 | 83.6 |
| MS.gene75650.t1 | AT5G42640 | 42.424 | 99 | 53 | 1 | 113 | 211 | 191 | 285 | 4.58e-18 | 82.4 |
Find 36 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGTCTTGAAGCACAATT+TGG | 0.219378 | 3.3:+70861901 | MS.gene75650:CDS |
| TCAGTTGGTACCAATAATTT+TGG | 0.290051 | 3.3:+70861847 | MS.gene75650:CDS |
| AGCATGACTACCAAAATTAT+TGG | 0.316188 | 3.3:-70861857 | None:intergenic |
| AGCCTACCACACAAGAAATA+TGG | 0.358104 | 3.3:+70862204 | MS.gene75650:CDS |
| TCTATGATTGTTCATATTAG+TGG | 0.384068 | 3.3:-70861779 | None:intergenic |
| GATTCAAGATGAGGAGGAAA+AGG | 0.404808 | 3.3:-70861754 | None:intergenic |
| TATTAATAATAATTATTTGA+AGG | 0.406891 | 3.3:-70862513 | None:intergenic |
| TTATCAGCACAAGCAATAAC+AGG | 0.430056 | 3.3:-70862408 | None:intergenic |
| ACAATCATAGATTGGTTCAA+TGG | 0.452424 | 3.3:+70861791 | MS.gene75650:CDS |
| TTCTAGGATTCGAAAGAGAC+CGG | 0.465129 | 3.3:+70862341 | MS.gene75650:CDS |
| GATAAGCCTACATGGAGATC+AGG | 0.469377 | 3.3:-70862054 | None:intergenic |
| AGCAATGAAAATATTGATGA+TGG | 0.472887 | 3.3:+70862171 | MS.gene75650:CDS |
| AGAGTCTTGAAGCACAATTT+GGG | 0.488266 | 3.3:+70861902 | MS.gene75650:CDS |
| ATTCAAGATGAGGAGGAAAA+GGG | 0.490874 | 3.3:-70861753 | None:intergenic |
| ATTTCTTGTGTGGTAGGCTG+TGG | 0.491007 | 3.3:-70862200 | None:intergenic |
| TAATATGAACAATCATAGAT+TGG | 0.492110 | 3.3:+70861783 | MS.gene75650:CDS |
| TTCAAGATGAGGAGGAAAAG+GGG | 0.498688 | 3.3:-70861752 | None:intergenic |
| TCACATCCTGATCTCCATGT+AGG | 0.522755 | 3.3:+70862048 | MS.gene75650:CDS |
| ATGATTGTTCATATTAGTGG+TGG | 0.523562 | 3.3:-70861776 | None:intergenic |
| TGAAGTGTGTTTAGAATCCA+TGG | 0.537305 | 3.3:-70861725 | None:intergenic |
| ACCAACTGATGATGATGAAT+GGG | 0.541850 | 3.3:-70861833 | None:intergenic |
| GTCCATATTTCTTGTGTGGT+AGG | 0.551362 | 3.3:-70862206 | None:intergenic |
| CTCTCACCCCTCTTCCAAGT+GGG | 0.558684 | 3.3:+70862472 | MS.gene75650:CDS |
| TACCAACTGATGATGATGAA+TGG | 0.562352 | 3.3:-70861834 | None:intergenic |
| CAAATTCAAAAGCTCAATGA+CGG | 0.582932 | 3.3:+70862369 | MS.gene75650:CDS |
| AGATTGGTTCAATGGATGCA+TGG | 0.584866 | 3.3:+70861799 | MS.gene75650:CDS |
| GCTCTCACCCCTCTTCCAAG+TGG | 0.584935 | 3.3:+70862471 | MS.gene75650:CDS |
| TATGGTCCATATTTCTTGTG+TGG | 0.586966 | 3.3:-70862210 | None:intergenic |
| ACCCATTCATCATCATCAGT+TGG | 0.590463 | 3.3:+70861832 | MS.gene75650:CDS |
| GTGGTGGATTCAAGATGAGG+AGG | 0.604335 | 3.3:-70861760 | None:intergenic |
| TCTAGGATTCGAAAGAGACC+GGG | 0.623769 | 3.3:+70862342 | MS.gene75650:CDS |
| TCCGGGAATCATAAAAGCGA+TGG | 0.628214 | 3.3:+70861879 | MS.gene75650:CDS |
| ATTGTGATGATAAGCCTACA+TGG | 0.633345 | 3.3:-70862062 | None:intergenic |
| TTAGTGGTGGATTCAAGATG+AGG | 0.640675 | 3.3:-70861763 | None:intergenic |
| GACGACGACGATGTTGATGT+AGG | 0.650410 | 3.3:-70861931 | None:intergenic |
| ATGTTAACAAGAACGATACG+AGG | 0.702248 | 3.3:+70862136 | MS.gene75650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATGAACAATCATAGAT+TGG | + | chr3.3:70861783-70861802 | MS.gene75650:CDS | 20.0% |
| ! | AGCAATGAAAATATTGATGA+TGG | + | chr3.3:70862171-70862190 | MS.gene75650:CDS | 25.0% |
| ! | GAAAAAAGAGATACATTTCT+AGG | + | chr3.3:70862325-70862344 | MS.gene75650:CDS | 25.0% |
| ! | TCTATGATTGTTCATATTAG+TGG | - | chr3.3:70861782-70861801 | None:intergenic | 25.0% |
| !! | ATTTTGTTGCATTTTGAACA+TGG | - | chr3.3:70862237-70862256 | None:intergenic | 25.0% |
| !!! | CAAACGATTAATATTTTTGC+TGG | + | chr3.3:70862102-70862121 | MS.gene75650:CDS | 25.0% |
| AGCATGACTACCAAAATTAT+TGG | - | chr3.3:70861860-70861879 | None:intergenic | 30.0% | |
| ATGATTGTTCATATTAGTGG+TGG | - | chr3.3:70861779-70861798 | None:intergenic | 30.0% | |
| CAAATTCAAAAGCTCAATGA+CGG | + | chr3.3:70862369-70862388 | MS.gene75650:CDS | 30.0% | |
| ! | ACAATCATAGATTGGTTCAA+TGG | + | chr3.3:70861791-70861810 | MS.gene75650:CDS | 30.0% |
| ! | TTGCATTTTGAACATGGATA+TGG | - | chr3.3:70862231-70862250 | None:intergenic | 30.0% |
| !! | TCAGTTGGTACCAATAATTT+TGG | + | chr3.3:70861847-70861866 | MS.gene75650:CDS | 30.0% |
| AGAGTCTTGAAGCACAATTT+GGG | + | chr3.3:70861902-70861921 | MS.gene75650:CDS | 35.0% | |
| ATGTTAACAAGAACGATACG+AGG | + | chr3.3:70862136-70862155 | MS.gene75650:CDS | 35.0% | |
| ATTCAAGATGAGGAGGAAAA+GGG | - | chr3.3:70861756-70861775 | None:intergenic | 35.0% | |
| ATTGTGATGATAAGCCTACA+TGG | - | chr3.3:70862065-70862084 | None:intergenic | 35.0% | |
| TATGGTCCATATTTCTTGTG+TGG | - | chr3.3:70862213-70862232 | None:intergenic | 35.0% | |
| TTATCAGCACAAGCAATAAC+AGG | - | chr3.3:70862411-70862430 | None:intergenic | 35.0% | |
| ! | ACCAACTGATGATGATGAAT+GGG | - | chr3.3:70861836-70861855 | None:intergenic | 35.0% |
| ! | AGCTTTTGAATTTGAAGACC+CGG | - | chr3.3:70862363-70862382 | None:intergenic | 35.0% |
| ! | TACCAACTGATGATGATGAA+TGG | - | chr3.3:70861837-70861856 | None:intergenic | 35.0% |
| !! | TAATTTTGGTAGTCATGCTC+CGG | + | chr3.3:70861861-70861880 | MS.gene75650:CDS | 35.0% |
| !! | TATTTTTGCTGGTGATGTTG+AGG | + | chr3.3:70862113-70862132 | MS.gene75650:CDS | 35.0% |
| ACCCATTCATCATCATCAGT+TGG | + | chr3.3:70861832-70861851 | MS.gene75650:CDS | 40.0% | |
| AGCCTACCACACAAGAAATA+TGG | + | chr3.3:70862204-70862223 | MS.gene75650:CDS | 40.0% | |
| GAGAGTCTTGAAGCACAATT+TGG | + | chr3.3:70861901-70861920 | MS.gene75650:CDS | 40.0% | |
| GATTCAAGATGAGGAGGAAA+AGG | - | chr3.3:70861757-70861776 | None:intergenic | 40.0% | |
| GTCCATATTTCTTGTGTGGT+AGG | - | chr3.3:70862209-70862228 | None:intergenic | 40.0% | |
| TTCAAGATGAGGAGGAAAAG+GGG | - | chr3.3:70861755-70861774 | None:intergenic | 40.0% | |
| ! | ACTTTTATCCCACTTGGAAG+AGG | - | chr3.3:70862483-70862502 | None:intergenic | 40.0% |
| ! | AGATTGGTTCAATGGATGCA+TGG | + | chr3.3:70861799-70861818 | MS.gene75650:CDS | 40.0% |
| ! | CTTTTATCCCACTTGGAAGA+GGG | - | chr3.3:70862482-70862501 | None:intergenic | 40.0% |
| ! | GACTTGACTTTTATCCCACT+TGG | - | chr3.3:70862489-70862508 | None:intergenic | 40.0% |
| ! | TTTTATCCCACTTGGAAGAG+GGG | - | chr3.3:70862481-70862500 | None:intergenic | 40.0% |
| !! | AATTTTGGTAGTCATGCTCC+GGG | + | chr3.3:70861862-70861881 | MS.gene75650:CDS | 40.0% |
| !! | TCCATCGCTTTTATGATTCC+CGG | - | chr3.3:70861883-70861902 | None:intergenic | 40.0% |
| !! | TTAGTGGTGGATTCAAGATG+AGG | - | chr3.3:70861766-70861785 | None:intergenic | 40.0% |
| !! | TTCTAGGATTCGAAAGAGAC+CGG | + | chr3.3:70862341-70862360 | MS.gene75650:CDS | 40.0% |
| ATTTCTTGTGTGGTAGGCTG+TGG | - | chr3.3:70862203-70862222 | None:intergenic | 45.0% | |
| GATAAGCCTACATGGAGATC+AGG | - | chr3.3:70862057-70862076 | None:intergenic | 45.0% | |
| TCACATCCTGATCTCCATGT+AGG | + | chr3.3:70862048-70862067 | MS.gene75650:CDS | 45.0% | |
| TCCGGGAATCATAAAAGCGA+TGG | + | chr3.3:70861879-70861898 | MS.gene75650:CDS | 45.0% | |
| ! | TCTAGGATTCGAAAGAGACC+GGG | + | chr3.3:70862342-70862361 | MS.gene75650:CDS | 45.0% |
| GACGACGACGATGTTGATGT+AGG | - | chr3.3:70861934-70861953 | None:intergenic | 50.0% | |
| !! | GTGGTGGATTCAAGATGAGG+AGG | - | chr3.3:70861763-70861782 | None:intergenic | 50.0% |
| CTCTCACCCCTCTTCCAAGT+GGG | + | chr3.3:70862472-70862491 | MS.gene75650:CDS | 55.0% | |
| GCTCTCACCCCTCTTCCAAG+TGG | + | chr3.3:70862471-70862490 | MS.gene75650:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 70861727 | 70862533 | 70861727 | ID=MS.gene75650 |
| chr3.3 | mRNA | 70861727 | 70862533 | 70861727 | ID=MS.gene75650.t1;Parent=MS.gene75650 |
| chr3.3 | exon | 70861727 | 70862533 | 70861727 | ID=MS.gene75650.t1.exon1;Parent=MS.gene75650.t1 |
| chr3.3 | CDS | 70861727 | 70862533 | 70861727 | ID=cds.MS.gene75650.t1;Parent=MS.gene75650.t1 |
| Gene Sequence |
| Protein sequence |
