Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75921.t1 | XP_018500604.1 | 60.4 | 111 | 42 | 1 | 1 | 109 | 88 | 198 | 2.90E-30 | 141.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75921.t1 | O65499 | 55.2 | 116 | 45 | 2 | 1 | 109 | 79 | 194 | 4.2e-30 | 132.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75921.t1 | A0A4P1RQC6 | 54.8 | 124 | 39 | 2 | 1 | 107 | 99 | 222 | 3.0e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene75921.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75921.t1 | MTR_5g099040 | 79.167 | 144 | 18 | 4 | 1 | 135 | 50 | 190 | 9.82e-71 | 214 |
| MS.gene75921.t1 | MTR_5g062300 | 82.353 | 119 | 12 | 3 | 1 | 111 | 36 | 153 | 7.21e-60 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75921.t1 | AT4G35280 | 55.970 | 134 | 38 | 4 | 1 | 119 | 79 | 206 | 3.90e-43 | 144 |
| MS.gene75921.t1 | AT2G17180 | 57.500 | 120 | 40 | 2 | 1 | 112 | 67 | 183 | 6.29e-39 | 133 |
| MS.gene75921.t1 | AT1G02040 | 46.250 | 80 | 30 | 1 | 41 | 107 | 101 | 180 | 4.12e-12 | 63.2 |
Find 46 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTCTTGTTCTTGTTCTT+TGG | 0.151007 | 5.1:-80667394 | None:intergenic |
| TCGTGTTGCAACAAAGTATT+TGG | 0.254538 | 5.1:+80667445 | MS.gene75921:CDS |
| GCAGCACCTTCCTGTTCTTC+TGG | 0.259557 | 5.1:-80667343 | None:intergenic |
| AGTTGTGGTCTTCTGAAATT+AGG | 0.270404 | 5.1:-80667304 | None:intergenic |
| CTGAATGCGGTAAGATATTC+TGG | 0.313666 | 5.1:+80667212 | MS.gene75921:CDS |
| CAAGGAATTGGAATTGGAAT+TGG | 0.326323 | 5.1:+80667607 | MS.gene75921:CDS |
| ACCCCTCAAGGAATTGGAAT+TGG | 0.366912 | 5.1:+80667601 | MS.gene75921:CDS |
| AGTAGTACCCCTCAAGGAAT+TGG | 0.394510 | 5.1:+80667595 | MS.gene75921:CDS |
| AACTGATCATCCACCACAAC+TGG | 0.401504 | 5.1:-80667415 | None:intergenic |
| ACAATAACCGCTTCTTCTAC+TGG | 0.405720 | 5.1:+80667544 | MS.gene75921:CDS |
| GTCTGAGGCACCATGTCATT+GGG | 0.418315 | 5.1:-80667666 | None:intergenic |
| AGTCTGAGGCACCATGTCAT+TGG | 0.446038 | 5.1:-80667667 | None:intergenic |
| CAAAGTATTTGGATCTCATC+AGG | 0.456499 | 5.1:+80667456 | MS.gene75921:CDS |
| GGAATTGGAATTGGAATTGG+AGG | 0.460082 | 5.1:+80667610 | MS.gene75921:CDS |
| ATTCCTCTCCATTGACGCTC+AGG | 0.461015 | 5.1:-80667271 | None:intergenic |
| TACCGCGACTCCAGAAGAAC+AGG | 0.473036 | 5.1:+80667333 | MS.gene75921:CDS |
| TCCTTGAGGGGTACTACTGC+TGG | 0.474800 | 5.1:-80667590 | None:intergenic |
| TTCCAATTCCAATTCCTTGA+GGG | 0.481350 | 5.1:-80667603 | None:intergenic |
| GGATCTCATCAGGCACTTGG+AGG | 0.512393 | 5.1:+80667466 | MS.gene75921:CDS |
| GTTGTCATCCTGAGCGTCAA+TGG | 0.514198 | 5.1:+80667263 | MS.gene75921:CDS |
| TTTGGATCTCATCAGGCACT+TGG | 0.527004 | 5.1:+80667463 | MS.gene75921:CDS |
| ATTGGAATTGGAATTGGAGG+AGG | 0.530886 | 5.1:+80667613 | MS.gene75921:CDS |
| TGTGGTCTTCTGAAATTAGG+AGG | 0.542449 | 5.1:-80667301 | None:intergenic |
| GCGGTAAGATATTCTGGTCA+TGG | 0.544652 | 5.1:+80667218 | MS.gene75921:CDS |
| ATCAGGCACTTGGAGGACAC+AGG | 0.546807 | 5.1:+80667473 | MS.gene75921:CDS |
| TCCAATTCCAATTCCTTGAG+GGG | 0.558425 | 5.1:-80667602 | None:intergenic |
| GTCTTCTGAAATTAGGAGGA+GGG | 0.563842 | 5.1:-80667297 | None:intergenic |
| GGTCTTCTGAAATTAGGAGG+AGG | 0.578908 | 5.1:-80667298 | None:intergenic |
| GGCTAGTCACAAGAATGTCA+AGG | 0.586833 | 5.1:+80667495 | MS.gene75921:CDS |
| ATTCCAATTCCAATTCCTTG+AGG | 0.590016 | 5.1:-80667604 | None:intergenic |
| CGTCCTTCCAGTAGAAGAAG+CGG | 0.594668 | 5.1:-80667551 | None:intergenic |
| TAAGATATTCTGGTCATGGA+AGG | 0.594766 | 5.1:+80667222 | MS.gene75921:CDS |
| TGCTCGTAGTGCACTGAATG+CGG | 0.596978 | 5.1:+80667199 | None:intergenic |
| TTCCTGTTCTTCTGGAGTCG+CGG | 0.607526 | 5.1:-80667335 | None:intergenic |
| GAACAAGAAACCAGTTGTGG+TGG | 0.608651 | 5.1:+80667405 | MS.gene75921:CDS |
| ACCAGCAGTAGTACCCCTCA+AGG | 0.626381 | 5.1:+80667589 | MS.gene75921:CDS |
| TAACCGCTTCTTCTACTGGA+AGG | 0.631386 | 5.1:+80667548 | MS.gene75921:CDS |
| GCTAGTCACAAGAATGTCAA+GGG | 0.632503 | 5.1:+80667496 | MS.gene75921:CDS |
| CATCCTGAGCGTCAATGGAG+AGG | 0.633209 | 5.1:+80667268 | MS.gene75921:CDS |
| GTCGCGGTATTGATGAGTTG+TGG | 0.636172 | 5.1:-80667319 | None:intergenic |
| TAACCGTCACCACGAGTCTG+AGG | 0.636568 | 5.1:-80667681 | None:intergenic |
| TCAGGCACTTGGAGGACACA+GGG | 0.641355 | 5.1:+80667474 | MS.gene75921:CDS |
| ACGATGACAACCCAATGACA+TGG | 0.663207 | 5.1:+80667656 | MS.gene75921:CDS |
| CAAGAACAAGAAACCAGTTG+TGG | 0.666899 | 5.1:+80667402 | MS.gene75921:CDS |
| GCGACTCCAGAAGAACAGGA+AGG | 0.732274 | 5.1:+80667337 | MS.gene75921:CDS |
| GAAGGACGTTCATGACGACG+TGG | 0.752983 | 5.1:+80667566 | MS.gene75921:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTGTTGTTGTTGTTGTAT+TGG | - | chr5.1:80667530-80667549 | None:intergenic | 25.0% |
| ATTCCAATTCCAATTCCTTG+AGG | - | chr5.1:80667607-80667626 | None:intergenic | 35.0% | |
| CAAGGAATTGGAATTGGAAT+TGG | + | chr5.1:80667607-80667626 | MS.gene75921:CDS | 35.0% | |
| GGTTTCTTGTTCTTGTTCTT+TGG | - | chr5.1:80667397-80667416 | None:intergenic | 35.0% | |
| TAAGATATTCTGGTCATGGA+AGG | + | chr5.1:80667222-80667241 | MS.gene75921:CDS | 35.0% | |
| TTCCAATTCCAATTCCTTGA+GGG | - | chr5.1:80667606-80667625 | None:intergenic | 35.0% | |
| ! | CAAAGTATTTGGATCTCATC+AGG | + | chr5.1:80667456-80667475 | MS.gene75921:CDS | 35.0% |
| ! | TCGTGTTGCAACAAAGTATT+TGG | + | chr5.1:80667445-80667464 | MS.gene75921:CDS | 35.0% |
| !! | AGTTGTGGTCTTCTGAAATT+AGG | - | chr5.1:80667307-80667326 | None:intergenic | 35.0% |
| ACAATAACCGCTTCTTCTAC+TGG | + | chr5.1:80667544-80667563 | MS.gene75921:CDS | 40.0% | |
| ATTGGAATTGGAATTGGAGG+AGG | + | chr5.1:80667613-80667632 | MS.gene75921:CDS | 40.0% | |
| CAAGAACAAGAAACCAGTTG+TGG | + | chr5.1:80667402-80667421 | MS.gene75921:CDS | 40.0% | |
| CTGAATGCGGTAAGATATTC+TGG | + | chr5.1:80667212-80667231 | MS.gene75921:CDS | 40.0% | |
| GCTAGTCACAAGAATGTCAA+GGG | + | chr5.1:80667496-80667515 | MS.gene75921:CDS | 40.0% | |
| GGAATTGGAATTGGAATTGG+AGG | + | chr5.1:80667610-80667629 | MS.gene75921:CDS | 40.0% | |
| TCCAATTCCAATTCCTTGAG+GGG | - | chr5.1:80667605-80667624 | None:intergenic | 40.0% | |
| !! | GTCTTCTGAAATTAGGAGGA+GGG | - | chr5.1:80667300-80667319 | None:intergenic | 40.0% |
| !! | TGTGGTCTTCTGAAATTAGG+AGG | - | chr5.1:80667304-80667323 | None:intergenic | 40.0% |
| AACTGATCATCCACCACAAC+TGG | - | chr5.1:80667418-80667437 | None:intergenic | 45.0% | |
| ACCCCTCAAGGAATTGGAAT+TGG | + | chr5.1:80667601-80667620 | MS.gene75921:CDS | 45.0% | |
| ACGATGACAACCCAATGACA+TGG | + | chr5.1:80667656-80667675 | MS.gene75921:CDS | 45.0% | |
| AGTAGTACCCCTCAAGGAAT+TGG | + | chr5.1:80667595-80667614 | MS.gene75921:CDS | 45.0% | |
| GAACAAGAAACCAGTTGTGG+TGG | + | chr5.1:80667405-80667424 | MS.gene75921:CDS | 45.0% | |
| GCGGTAAGATATTCTGGTCA+TGG | + | chr5.1:80667218-80667237 | MS.gene75921:CDS | 45.0% | |
| TAACCGCTTCTTCTACTGGA+AGG | + | chr5.1:80667548-80667567 | MS.gene75921:CDS | 45.0% | |
| TTTGGATCTCATCAGGCACT+TGG | + | chr5.1:80667463-80667482 | MS.gene75921:CDS | 45.0% | |
| ! | GGCTAGTCACAAGAATGTCA+AGG | + | chr5.1:80667495-80667514 | MS.gene75921:CDS | 45.0% |
| ! | TGGTCATGGAAGGCTCTTTT+CGG | + | chr5.1:80667232-80667251 | MS.gene75921:CDS | 45.0% |
| !! | GGTCTTCTGAAATTAGGAGG+AGG | - | chr5.1:80667301-80667320 | None:intergenic | 45.0% |
| ATTCCTCTCCATTGACGCTC+AGG | - | chr5.1:80667274-80667293 | None:intergenic | 50.0% | |
| CGTCCTTCCAGTAGAAGAAG+CGG | - | chr5.1:80667554-80667573 | None:intergenic | 50.0% | |
| GTTGTCATCCTGAGCGTCAA+TGG | + | chr5.1:80667263-80667282 | MS.gene75921:CDS | 50.0% | |
| ! | AGTCTGAGGCACCATGTCAT+TGG | - | chr5.1:80667670-80667689 | None:intergenic | 50.0% |
| ! | GTCTGAGGCACCATGTCATT+GGG | - | chr5.1:80667669-80667688 | None:intergenic | 50.0% |
| !! | GTCGCGGTATTGATGAGTTG+TGG | - | chr5.1:80667322-80667341 | None:intergenic | 50.0% |
| !! | TTCCTGTTCTTCTGGAGTCG+CGG | - | chr5.1:80667338-80667357 | None:intergenic | 50.0% |
| ACCAGCAGTAGTACCCCTCA+AGG | + | chr5.1:80667589-80667608 | MS.gene75921:CDS | 55.0% | |
| ATCAGGCACTTGGAGGACAC+AGG | + | chr5.1:80667473-80667492 | MS.gene75921:CDS | 55.0% | |
| CATCCTGAGCGTCAATGGAG+AGG | + | chr5.1:80667268-80667287 | MS.gene75921:CDS | 55.0% | |
| GAAGGACGTTCATGACGACG+TGG | + | chr5.1:80667566-80667585 | MS.gene75921:CDS | 55.0% | |
| GCAGCACCTTCCTGTTCTTC+TGG | - | chr5.1:80667346-80667365 | None:intergenic | 55.0% | |
| GCGACTCCAGAAGAACAGGA+AGG | + | chr5.1:80667337-80667356 | MS.gene75921:CDS | 55.0% | |
| GGATCTCATCAGGCACTTGG+AGG | + | chr5.1:80667466-80667485 | MS.gene75921:CDS | 55.0% | |
| TACCGCGACTCCAGAAGAAC+AGG | + | chr5.1:80667333-80667352 | MS.gene75921:CDS | 55.0% | |
| TCAGGCACTTGGAGGACACA+GGG | + | chr5.1:80667474-80667493 | MS.gene75921:CDS | 55.0% | |
| !! | TCCTTGAGGGGTACTACTGC+TGG | - | chr5.1:80667593-80667612 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 80667208 | 80667690 | 80667208 | ID=MS.gene75921 |
| chr5.1 | mRNA | 80667208 | 80667690 | 80667208 | ID=MS.gene75921.t1;Parent=MS.gene75921 |
| chr5.1 | exon | 80667208 | 80667690 | 80667208 | ID=MS.gene75921.t1.exon1;Parent=MS.gene75921.t1 |
| chr5.1 | CDS | 80667208 | 80667690 | 80667208 | ID=cds.MS.gene75921.t1;Parent=MS.gene75921.t1 |
| Gene Sequence |
| Protein sequence |
