AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76044

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76044.t1 MTR_1g098870 96.655 568 16 1 1 568 1 565 0.0 1141
MS.gene76044.t1 MTR_1g098900 45.923 601 204 15 3 500 2 584 7.41e-153 453
MS.gene76044.t1 MTR_6g035370 54.167 288 111 3 52 318 52 339 1.23e-73 247
MS.gene76044.t1 MTR_6g035370 58.642 162 61 2 363 521 449 607 3.03e-46 172
MS.gene76044.t1 MTR_6g035370 43.617 94 52 1 51 143 460 553 1.08e-18 90.1
MS.gene76044.t1 MTR_6g035370 41.379 87 50 1 375 461 55 140 7.19e-17 84.3
MS.gene76044.t1 MTR_1g492790 30.118 425 222 13 36 432 95 472 1.36e-46 171
MS.gene76044.t1 MTR_1g492790 38.129 139 81 3 329 466 71 205 5.35e-20 93.6
MS.gene76044.t1 MTR_2g026725 37.860 243 123 4 241 463 116 350 1.65e-43 159
MS.gene76044.t1 MTR_2g026725 39.091 110 59 2 54 162 261 363 5.12e-16 80.5
MS.gene76044.t1 MTR_1g060990 34.354 294 161 5 55 343 131 397 2.01e-41 157
MS.gene76044.t1 MTR_1g060990 42.202 109 61 2 364 471 120 227 3.92e-20 94.4
MS.gene76044.t1 MTR_1g081180 33.582 268 127 6 239 464 251 509 1.80e-33 134
MS.gene76044.t1 MTR_1g081180 40.426 94 53 2 53 143 416 509 4.35e-14 75.5
MS.gene76044.t1 MTR_7g114860 31.174 247 142 7 240 465 241 480 4.09e-29 121
MS.gene76044.t1 MTR_7g114860 43.434 99 46 2 55 143 381 479 4.58e-16 81.3
MS.gene76044.t1 MTR_5g026540 29.323 133 82 3 335 465 10 132 4.02e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76044.t1 AT1G76890 49.797 492 181 10 50 496 36 506 3.53e-142 423
MS.gene76044.t1 AT1G76880 52.183 481 194 8 50 497 56 533 2.29e-137 412
MS.gene76044.t1 AT5G03680 34.038 426 235 12 55 462 119 516 5.54e-60 209
MS.gene76044.t1 AT5G28300 38.224 259 126 6 240 464 300 558 2.53e-40 155
MS.gene76044.t1 AT3G10000 32.239 335 161 10 12 323 56 347 1.01e-38 149
MS.gene76044.t1 AT3G10000 35.915 142 81 3 343 477 53 191 5.49e-21 96.7
MS.gene76044.t1 AT1G33240 68.132 91 29 0 375 465 435 525 1.04e-37 147
MS.gene76044.t1 AT1G33240 68.132 91 29 0 375 465 396 486 1.26e-37 147
MS.gene76044.t1 AT1G33240 43.678 87 48 1 55 140 396 482 1.75e-16 83.2
MS.gene76044.t1 AT1G33240 68.132 91 29 0 375 465 435 525 2.30e-37 147
MS.gene76044.t1 AT3G10000 31.138 334 156 10 12 323 56 337 5.53e-35 138
MS.gene76044.t1 AT3G10000 34.043 141 75 3 343 477 53 181 6.37e-18 87.0
MS.gene76044.t1 AT5G47660 31.718 227 140 4 238 463 177 389 1.77e-27 115
MS.gene76044.t1 AT5G47660 41.304 92 51 2 55 145 303 392 4.14e-13 71.6
MS.gene76044.t1 AT3G25990 32.000 100 67 1 376 474 55 154 5.74e-11 65.1

Find 118 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAGAAAGAAGAGAAAA+TGG 0.189121 1.1:+71072675 MS.gene76044:CDS
GAACCAAAGTATCAAGAAAA+TGG 0.192524 1.1:+71073154 MS.gene76044:CDS
TTCATGTTTGGCAAAGAATT+TGG 0.196846 1.1:-71072584 None:intergenic
TTATTATTTGGTTGTGGTTT+TGG 0.205052 1.1:-71072431 None:intergenic
GAAAATGGAAGGACTATTTC+AGG 0.211748 1.1:+71072690 MS.gene76044:CDS
GGAGGTCTCCATTGTTGTTC+TGG 0.263104 1.1:-71073457 None:intergenic
GAATTTCAAAATTTGAATAA+TGG 0.276547 1.1:+71073040 MS.gene76044:CDS
TCTTCCCACAATGGCGCTTT+TGG 0.281382 1.1:-71073178 None:intergenic
GAGCTGCTGATGATCTTTCT+TGG 0.298176 1.1:-71072865 None:intergenic
GTGAAGTTCATGCTCTAATA+AGG 0.301161 1.1:+71073116 MS.gene76044:CDS
GCTTCAAGTTCATTAAAATA+AGG 0.305695 1.1:-71073337 None:intergenic
GATGAGAGTGTGTTATTATT+TGG 0.316885 1.1:-71072443 None:intergenic
TCAAGAGAGCCAATGTTTCT+TGG 0.332675 1.1:-71071326 None:intergenic
GAGAATAGGTTGTTCATGTT+TGG 0.353362 1.1:-71072596 None:intergenic
TTAACGGTGGTGGAGGAAAT+AGG 0.356278 1.1:+71071295 MS.gene76044:CDS
AACTTCTTCTTCCACAGCTT+CGG 0.361209 1.1:+71072634 MS.gene76044:CDS
GAAACGAATTAGAACCAAAA+AGG 0.368019 1.1:-71073390 None:intergenic
TATTGTTTGTGTGGTTTCAT+TGG 0.368826 1.1:-71072480 None:intergenic
CTTGAAGATAAGATCAGATA+TGG 0.382626 1.1:+71071344 MS.gene76044:CDS
TTGAATAATGGTTACAAAAG+TGG 0.392274 1.1:+71073052 MS.gene76044:CDS
GCTGGCGGTGGTTGTGGTGG+TGG 0.395680 1.1:-71072935 None:intergenic
GTTTCATTGGGATTTGTTGT+TGG 0.399933 1.1:-71072467 None:intergenic
AAGTCTTAAGGGTCCACTTT+GGG 0.401378 1.1:+71071386 MS.gene76044:CDS
TCTGGTGCTGGCGGTGGTTG+TGG 0.405288 1.1:-71072941 None:intergenic
AACGAATGTGGTAGGGTTTG+TGG 0.409596 1.1:-71072528 None:intergenic
TGAGAATATTGTGCCGGTTA+CGG 0.409713 1.1:+71071197 MS.gene76044:CDS
GAAGAGAAAGGAAGAGAGAT+TGG 0.412336 1.1:+71071240 MS.gene76044:CDS
CACAGCTTCGGACGAAGACT+TGG 0.417011 1.1:+71072646 MS.gene76044:CDS
TGTTCTGGCTGCACCATCAA+TGG 0.420164 1.1:-71073442 None:intergenic
ATTGTTTGTGTGGTTTCATT+GGG 0.421975 1.1:-71072479 None:intergenic
TGCTTTGAGAATTCAAGAAA+TGG 0.425606 1.1:+71072811 MS.gene76044:CDS
AATTCAACAATGTTGTTGTT+TGG 0.432386 1.1:-71073022 None:intergenic
TTTGGAACATTGTTGTTGTT+AGG 0.434536 1.1:-71072566 None:intergenic
CACTTTGGGAAGAGGTTTCA+AGG 0.436137 1.1:+71071400 MS.gene76044:CDS
AACAAATAAACTTTCATCAA+TGG 0.444317 1.1:+71073651 MS.gene76044:CDS
GACAGCTCTGGTGCTGGCGG+TGG 0.444859 1.1:-71072947 None:intergenic
AAGTTGTTGTGGTTGAGAAT+AGG 0.446298 1.1:-71072610 None:intergenic
AACCAAAGTATCAAGAAAAT+GGG 0.447699 1.1:+71073155 MS.gene76044:CDS
TGAAGAGACAAGGATACAAT+AGG 0.451641 1.1:+71073215 MS.gene76044:CDS
ATCAATATTAACGGTGGTGG+AGG 0.457605 1.1:+71071288 MS.gene76044:CDS
GTGCTTCTCCTTCTCCATCA+AGG 0.462199 1.1:+71073083 MS.gene76044:CDS
GAGAGCCAATGTTTCTTGGC+GGG 0.464598 1.1:-71071322 None:intergenic
AATGGTTACAAAAGTGGTAA+TGG 0.466799 1.1:+71073058 MS.gene76044:CDS
CACGGAGACAGCTCTGGTGC+TGG 0.472655 1.1:-71072953 None:intergenic
TAAGGGTCCACTTTGGGAAG+AGG 0.472903 1.1:+71071392 MS.gene76044:CDS
TTTCGTGATTCAAGTCTTAA+GGG 0.478392 1.1:+71071375 MS.gene76044:CDS
AAGATGAATATCAATATTAA+CGG 0.478883 1.1:+71071279 MS.gene76044:CDS
ACATTGTTGTTGTTAGGTTG+TGG 0.486612 1.1:-71072560 None:intergenic
AGGAAGAGAGATTGGTGGTG+AGG 0.490326 1.1:+71071248 MS.gene76044:CDS
TGAGACGATGGAGCCATTGA+TGG 0.495814 1.1:+71073429 MS.gene76044:CDS
CAAGTCTTAAGGGTCCACTT+TGG 0.495967 1.1:+71071385 MS.gene76044:CDS
ATCTTCTTCTATATGCAGCT+TGG 0.501249 1.1:+71071141 None:intergenic
GGTGCTGGCGGTGGTTGTGG+TGG 0.502284 1.1:-71072938 None:intergenic
GCACCCTCTTCAAATGTAGT+TGG 0.502758 1.1:-71073478 None:intergenic
GTCTGACACGGAGACAGCTC+TGG 0.505511 1.1:-71072959 None:intergenic
AATATCAATATTAACGGTGG+TGG 0.506001 1.1:+71071285 MS.gene76044:CDS
CCCTCTTCAAATGTAGTTGG+AGG 0.507603 1.1:-71073475 None:intergenic
TGTAGGAAACTAGCTGAACT+TGG 0.509833 1.1:+71072245 MS.gene76044:intron
GTCTCCGTGTCAGACAGCTC+TGG 0.513072 1.1:+71072967 MS.gene76044:CDS
TAGAAAGAAGAGAAAATGGA+AGG 0.520671 1.1:+71072679 MS.gene76044:CDS
GAAGGAGAAACGAATGTGGT+AGG 0.521206 1.1:-71072536 None:intergenic
TGGGCCAAAAGCGCCATTGT+GGG 0.524060 1.1:+71073174 MS.gene76044:CDS
AGGGTTTGTGGATGGAACAG+TGG 0.526949 1.1:-71072516 None:intergenic
CAAGTCTTCGTCCGAAGCTG+TGG 0.529430 1.1:-71072645 None:intergenic
GATAGCATGGAAGAAGATGA+AGG 0.531181 1.1:+71073598 MS.gene76044:CDS
AGTGTGTTATTATTTGGTTG+TGG 0.532548 1.1:-71072437 None:intergenic
GACATATGATATTGTTTGTG+TGG 0.533682 1.1:-71072489 None:intergenic
GTGCCGGTTACGGCGCAAAA+CGG 0.534516 1.1:+71071207 MS.gene76044:CDS
ACGGCGCAAAACGGAGGCGG+CGG 0.535961 1.1:+71071216 MS.gene76044:CDS
TTTGGCCACCTTGATGGAGA+AGG 0.538200 1.1:-71073091 None:intergenic
TATCACAAAAGAACCAAAGA+AGG 0.539271 1.1:+71072320 MS.gene76044:CDS
CTCCGGTGAGAATATTGTGC+CGG 0.540412 1.1:+71071191 MS.gene76044:CDS
ATATGAAGATGGAGATAGCA+TGG 0.544034 1.1:+71073585 MS.gene76044:CDS
AGAGCCAATGTTTCTTGGCG+GGG 0.547860 1.1:-71071321 None:intergenic
TTGTTGTCACAACTTCATAA+CGG 0.553559 1.1:-71073633 None:intergenic
CTTGAAACCTCTTCCCAAAG+TGG 0.553632 1.1:-71071399 None:intergenic
GGTAGGAGTGGAAAATCAGA+AGG 0.553996 1.1:+71072341 MS.gene76044:CDS
TGGTGATGCAACAGTAATGA+TGG 0.554542 1.1:+71071161 MS.gene76044:CDS
ATGGGCCAAAAGCGCCATTG+TGG 0.554967 1.1:+71073173 MS.gene76044:CDS
GAGAAAGGAAGAGAGATTGG+TGG 0.555860 1.1:+71071243 MS.gene76044:CDS
TTGTTGTTAGGTTGTGGTGA+AGG 0.559792 1.1:-71072554 None:intergenic
GGAGGCGGCGGTGAAGAGAA+AGG 0.561124 1.1:+71071228 MS.gene76044:CDS
GTTACGGCGCAAAACGGAGG+CGG 0.565758 1.1:+71071213 MS.gene76044:CDS
AACATGGATGAATATGAAGA+TGG 0.566553 1.1:+71073574 MS.gene76044:CDS
AGAAGAAGTTGAAGTTGTTG+TGG 0.571331 1.1:-71072621 None:intergenic
AAGGAGAAACGAATGTGGTA+GGG 0.571339 1.1:-71072535 None:intergenic
AGAGAGCCAATGTTTCTTGG+CGG 0.579042 1.1:-71071323 None:intergenic
CAAATACTACAAGAAAATGA+AGG 0.579174 1.1:+71073276 MS.gene76044:CDS
TGAAGGAAAGTAACAAGCAA+AGG 0.584180 1.1:+71073293 MS.gene76044:CDS
GTGGCCCCGCCAAGAAACAT+TGG 0.588347 1.1:+71071317 MS.gene76044:CDS
GGAGACAGCTCTGGTGCTGG+CGG 0.588687 1.1:-71072950 None:intergenic
GATACAATAGGAATGCGAAA+AGG 0.589387 1.1:+71073227 MS.gene76044:CDS
TGATGAAGAAGATGAAAACA+TGG 0.596066 1.1:+71073558 MS.gene76044:CDS
AGAACCAAAGAAGGTAGGAG+TGG 0.596126 1.1:+71072329 MS.gene76044:CDS
ACCTCCAACTACATTTGAAG+AGG 0.596492 1.1:+71073474 MS.gene76044:CDS
AAGATAAGATCAGATATGGA+TGG 0.598844 1.1:+71071348 MS.gene76044:CDS
AATGCAAAAGAAGTTTCTTG+AGG 0.603327 1.1:+71072748 MS.gene76044:CDS
ACAAGTGCTTGCAAAACAAG+AGG 0.605374 1.1:+71072724 MS.gene76044:CDS
GTAATGATGGAGACTTGCTC+CGG 0.605776 1.1:+71071174 MS.gene76044:CDS
TAGAAGCTCAAAGAAGTGCA+AGG 0.606310 1.1:+71072274 MS.gene76044:CDS
ACAAAAGAACCAAAGAAGGT+AGG 0.608634 1.1:+71072324 MS.gene76044:CDS
AACCGGCACAATATTCTCAC+CGG 0.619642 1.1:-71071193 None:intergenic
TGGTGCAGCCAGAACAACAA+TGG 0.627830 1.1:+71073449 MS.gene76044:CDS
CCGGTTACGGCGCAAAACGG+AGG 0.631559 1.1:+71071210 MS.gene76044:CDS
GCAAAAGAAGAAGATGACAA+TGG 0.642786 1.1:+71073526 MS.gene76044:CDS
AGCATGGAAGAAGATGAAGG+AGG 0.648293 1.1:+71073601 MS.gene76044:CDS
ATGAATATCAATATTAACGG+TGG 0.649447 1.1:+71071282 MS.gene76044:CDS
CATGAAGTCAAATGAGACGA+TGG 0.663039 1.1:+71073417 MS.gene76044:CDS
TACATTTGAAGAGGGTGCTG+TGG 0.663974 1.1:+71073483 MS.gene76044:CDS
GCTGAAATATCTTCCCACAA+TGG 0.664092 1.1:-71073187 None:intergenic
CCTCCAACTACATTTGAAGA+GGG 0.665021 1.1:+71073475 MS.gene76044:CDS
GGTTACAAAAGTGGTAATGG+TGG 0.667767 1.1:+71073061 MS.gene76044:CDS
AATGTGGTAGGGTTTGTGGA+TGG 0.673197 1.1:-71072524 None:intergenic
CTTCTCCTTCTCCATCAAGG+TGG 0.673995 1.1:+71073086 MS.gene76044:CDS
TCAGCTGCAATGAAGAGACA+AGG 0.690286 1.1:+71073205 MS.gene76044:CDS
GATGCCAGAGCTGTCTGACA+CGG 0.707672 1.1:-71072971 None:intergenic
TGGTGAAGGAGAAACGAATG+TGG 0.713404 1.1:-71072540 None:intergenic
ACGGTGGTGGAGGAAATAGG+TGG 0.747281 1.1:+71071298 MS.gene76044:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACCAAAAAAAAATTATATA+TGG - chr1.1:71071818-71071837 None:intergenic 10.0%
!! TTAAACTTAAGTAATTTAAT+TGG - chr1.1:71071869-71071888 None:intergenic 10.0%
!!! TGTGTTTTTTTTTTTTTTTT+TGG + chr1.1:71072008-71072027 MS.gene76044:intron 10.0%
!! AAGATGAATATCAATATTAA+CGG + chr1.1:71071279-71071298 MS.gene76044:CDS 15.0%
!! ACAAAGTAATAAAAATTAGA+TGG - chr1.1:71071938-71071957 None:intergenic 15.0%
!! GAATTTCAAAATTTGAATAA+TGG + chr1.1:71073040-71073059 MS.gene76044:CDS 15.0%
!! GATATATAAATATTGAATTG+AGG - chr1.1:71071429-71071448 None:intergenic 15.0%
!! TAATTGATCAAAAAATCTAT+AGG - chr1.1:71072372-71072391 None:intergenic 15.0%
!!! CTCCATATATAATTTTTTTT+TGG + chr1.1:71071813-71071832 MS.gene76044:intron 15.0%
!! AAAAGTAAAAATTTGTCATG+AGG - chr1.1:71072077-71072096 None:intergenic 20.0%
!! AACAAATAAACTTTCATCAA+TGG + chr1.1:71073651-71073670 MS.gene76044:CDS 20.0%
!! AGTAATAAAAATTAGATGGA+AGG - chr1.1:71071934-71071953 None:intergenic 20.0%
!! CTTATAAACAAGAAAATGAA+AGG - chr1.1:71071501-71071520 None:intergenic 20.0%
!!! TTTGTGTGTTTCTTATTTAT+TGG + chr1.1:71071953-71071972 MS.gene76044:intron 20.0%
!!! TTTTACTTTTTTTACGTGAA+AGG + chr1.1:71072087-71072106 MS.gene76044:intron 20.0%
!!! TTTTTCTTTTCCTTTTTCAA+TGG + chr1.1:71072118-71072137 MS.gene76044:intron 20.0%
!!! TTTTTTTTTTCCTTCTGAAA+AGG + chr1.1:71071638-71071657 MS.gene76044:intron 20.0%
! AAAAAAAAACACATGAAGCT+AGG - chr1.1:71072001-71072020 None:intergenic 25.0%
! AGTATAGAAAGAAGAGAAAA+TGG + chr1.1:71072675-71072694 MS.gene76044:CDS 25.0%
! ATGAATATCAATATTAACGG+TGG + chr1.1:71071282-71071301 MS.gene76044:CDS 25.0%
! CAAATACTACAAGAAAATGA+AGG + chr1.1:71073276-71073295 MS.gene76044:CDS 25.0%
! CCTACATGTATATATATAGA+GGG - chr1.1:71072231-71072250 None:intergenic 25.0%
! GTATATATATAGAGGGATTA+AGG - chr1.1:71072224-71072243 None:intergenic 25.0%
! GTTAGTATATCCATTGAAAA+AGG - chr1.1:71072131-71072150 None:intergenic 25.0%
! TCCTACATGTATATATATAG+AGG - chr1.1:71072232-71072251 None:intergenic 25.0%
! TTGAATAATGGTTACAAAAG+TGG + chr1.1:71073052-71073071 MS.gene76044:CDS 25.0%
!! AACCAAAGTATCAAGAAAAT+GGG + chr1.1:71073155-71073174 MS.gene76044:CDS 25.0%
!! AATTCAACAATGTTGTTGTT+TGG - chr1.1:71073025-71073044 None:intergenic 25.0%
!! CAAAAACTGATTGGTTAATT+TGG - chr1.1:71071564-71071583 None:intergenic 25.0%
!! CAAATTAACCAATCAGTTTT+TGG + chr1.1:71071562-71071581 MS.gene76044:intron 25.0%
!! GCTTCAAGTTCATTAAAATA+AGG - chr1.1:71073340-71073359 None:intergenic 25.0%
!! TATTTATTGGTCACTCTTTT+AGG + chr1.1:71071966-71071985 MS.gene76044:intron 25.0%
!! TTATTATTTGGTTGTGGTTT+TGG - chr1.1:71072434-71072453 None:intergenic 25.0%
!! TTCAGTTATCTTAGATTTTG+AGG + chr1.1:71071695-71071714 MS.gene76044:intron 25.0%
!!! CATCTTCTTCAACTTTTTTT+AGG + chr1.1:71071898-71071917 MS.gene76044:intron 25.0%
AACATGGATGAATATGAAGA+TGG + chr1.1:71073574-71073593 MS.gene76044:CDS 30.0%
AAGATAAGATCAGATATGGA+TGG + chr1.1:71071348-71071367 MS.gene76044:CDS 30.0%
AATAGGTTCCAAAAACTGAT+TGG - chr1.1:71071573-71071592 None:intergenic 30.0%
AATATCAATATTAACGGTGG+TGG + chr1.1:71071285-71071304 MS.gene76044:CDS 30.0%
AATGCAAAAGAAGTTTCTTG+AGG + chr1.1:71072748-71072767 MS.gene76044:CDS 30.0%
AATGGTTACAAAAGTGGTAA+TGG + chr1.1:71073058-71073077 MS.gene76044:CDS 30.0%
ATCTTATTCTCAAGTGTATG+AGG + chr1.1:71071666-71071685 MS.gene76044:intron 30.0%
ATTGTTTGTGTGGTTTCATT+GGG - chr1.1:71072482-71072501 None:intergenic 30.0%
CCCTCTATATATATACATGT+AGG + chr1.1:71072228-71072247 MS.gene76044:intron 30.0%
CTTGAAGATAAGATCAGATA+TGG + chr1.1:71071344-71071363 MS.gene76044:CDS 30.0%
GAAACGAATTAGAACCAAAA+AGG - chr1.1:71073393-71073412 None:intergenic 30.0%
GACATATGATATTGTTTGTG+TGG - chr1.1:71072492-71072511 None:intergenic 30.0%
GTCAAAAAAAGCTCTTTGAT+TGG + chr1.1:71071610-71071629 MS.gene76044:intron 30.0%
TAGAAAGAAGAGAAAATGGA+AGG + chr1.1:71072679-71072698 MS.gene76044:CDS 30.0%
TATCACAAAAGAACCAAAGA+AGG + chr1.1:71072320-71072339 MS.gene76044:CDS 30.0%
TATTGTTTGTGTGGTTTCAT+TGG - chr1.1:71072483-71072502 None:intergenic 30.0%
TGATGAAGAAGATGAAAACA+TGG + chr1.1:71073558-71073577 MS.gene76044:CDS 30.0%
TTCATGTTTGGCAAAGAATT+TGG - chr1.1:71072587-71072606 None:intergenic 30.0%
TTGTTGTCACAACTTCATAA+CGG - chr1.1:71073636-71073655 None:intergenic 30.0%
TTTCGTGATTCAAGTCTTAA+GGG + chr1.1:71071375-71071394 MS.gene76044:CDS 30.0%
! AACAAAACACAGAACCTTTT+TGG + chr1.1:71073376-71073395 MS.gene76044:CDS 30.0%
! AAGATATATGCCTTTTCAGA+AGG - chr1.1:71071651-71071670 None:intergenic 30.0%
! AGTGTGTTATTATTTGGTTG+TGG - chr1.1:71072440-71072459 None:intergenic 30.0%
! GAAAAGGTGCAAAGAAAAAT+GGG + chr1.1:71073243-71073262 MS.gene76044:CDS 30.0%
! GAACCAAAGTATCAAGAAAA+TGG + chr1.1:71073154-71073173 MS.gene76044:CDS 30.0%
! GATGAGAGTGTGTTATTATT+TGG - chr1.1:71072446-71072465 None:intergenic 30.0%
! TTTGGAACATTGTTGTTGTT+AGG - chr1.1:71072569-71072588 None:intergenic 30.0%
! TTTTCGTGATTCAAGTCTTA+AGG + chr1.1:71071374-71071393 MS.gene76044:CDS 30.0%
!! TGCTTTGAGAATTCAAGAAA+TGG + chr1.1:71072811-71072830 MS.gene76044:CDS 30.0%
AAGTTGTTGTGGTTGAGAAT+AGG - chr1.1:71072613-71072632 None:intergenic 35.0%
ACAAAAGAACCAAAGAAGGT+AGG + chr1.1:71072324-71072343 MS.gene76044:CDS 35.0%
AGAAGAAGTTGAAGTTGTTG+TGG - chr1.1:71072624-71072643 None:intergenic 35.0%
ATATAGAGGGATTAAGGAAG+TGG - chr1.1:71072218-71072237 None:intergenic 35.0%
ATATGAAGATGGAGATAGCA+TGG + chr1.1:71073585-71073604 MS.gene76044:CDS 35.0%
GAAAATGGAAGGACTATTTC+AGG + chr1.1:71072690-71072709 MS.gene76044:CDS 35.0%
GAGAATAGGTTGTTCATGTT+TGG - chr1.1:71072599-71072618 None:intergenic 35.0%
GATACAATAGGAATGCGAAA+AGG + chr1.1:71073227-71073246 MS.gene76044:CDS 35.0%
GCAAAAGAAGAAGATGACAA+TGG + chr1.1:71073526-71073545 MS.gene76044:CDS 35.0%
GCATTTCTCCAAAAATTGTC+TGG + chr1.1:71072908-71072927 MS.gene76044:CDS 35.0%
GTGAAGTTCATGCTCTAATA+AGG + chr1.1:71073116-71073135 MS.gene76044:CDS 35.0%
TGAAGAGACAAGGATACAAT+AGG + chr1.1:71073215-71073234 MS.gene76044:CDS 35.0%
TGAAGGAAAGTAACAAGCAA+AGG + chr1.1:71073293-71073312 MS.gene76044:CDS 35.0%
TTCTTCTATCTGAATCTTGC+TGG + chr1.1:71071757-71071776 MS.gene76044:intron 35.0%
! ACATTGTTGTTGTTAGGTTG+TGG - chr1.1:71072563-71072582 None:intergenic 35.0%
! CGAAAAGGTGCAAAGAAAAA+TGG + chr1.1:71073242-71073261 MS.gene76044:CDS 35.0%
!! AGAGCATGAACTTCACTTTT+TGG - chr1.1:71073112-71073131 None:intergenic 35.0%
!! GTTTCATTGGGATTTGTTGT+TGG - chr1.1:71072470-71072489 None:intergenic 35.0%
!!! TATTTGGTTGTGGTTTTGGA+TGG - chr1.1:71072430-71072449 None:intergenic 35.0%
AACTTCTTCTTCCACAGCTT+CGG + chr1.1:71072634-71072653 MS.gene76044:CDS 40.0%
AAGGAGAAACGAATGTGGTA+GGG - chr1.1:71072538-71072557 None:intergenic 40.0%
AAGTCTTAAGGGTCCACTTT+GGG + chr1.1:71071386-71071405 MS.gene76044:CDS 40.0%
ACAAGTGCTTGCAAAACAAG+AGG + chr1.1:71072724-71072743 MS.gene76044:CDS 40.0%
ACCTCCAACTACATTTGAAG+AGG + chr1.1:71073474-71073493 MS.gene76044:CDS 40.0%
CATGAAGTCAAATGAGACGA+TGG + chr1.1:71073417-71073436 MS.gene76044:CDS 40.0%
CCTCCAACTACATTTGAAGA+GGG + chr1.1:71073475-71073494 MS.gene76044:CDS 40.0%
GAAGAGAAAGGAAGAGAGAT+TGG + chr1.1:71071240-71071259 MS.gene76044:CDS 40.0%
GATAGCATGGAAGAAGATGA+AGG + chr1.1:71073598-71073617 MS.gene76044:CDS 40.0%
GCTGAAATATCTTCCCACAA+TGG - chr1.1:71073190-71073209 None:intergenic 40.0%
TAGTTCCACTGACAGGAAAT+AGG - chr1.1:71071590-71071609 None:intergenic 40.0%
TCAAGAGAGCCAATGTTTCT+TGG - chr1.1:71071329-71071348 None:intergenic 40.0%
TGTAGGAAACTAGCTGAACT+TGG + chr1.1:71072245-71072264 MS.gene76044:intron 40.0%
! ATCAATATTAACGGTGGTGG+AGG + chr1.1:71071288-71071307 MS.gene76044:CDS 40.0%
! GGCCCATTTTCTTGATACTT+TGG - chr1.1:71073160-71073179 None:intergenic 40.0%
! TAGAAGCTCAAAGAAGTGCA+AGG + chr1.1:71072274-71072293 MS.gene76044:CDS 40.0%
! TGGTGATGCAACAGTAATGA+TGG + chr1.1:71071161-71071180 MS.gene76044:CDS 40.0%
! TTGTTGTTAGGTTGTGGTGA+AGG - chr1.1:71072557-71072576 None:intergenic 40.0%
! TTTTCCACTCCTACCTTCTT+TGG - chr1.1:71072336-71072355 None:intergenic 40.0%
!! GGTTACAAAAGTGGTAATGG+TGG + chr1.1:71073061-71073080 MS.gene76044:CDS 40.0%
!! TGAGAATATTGTGCCGGTTA+CGG + chr1.1:71071197-71071216 MS.gene76044:CDS 40.0%
!! TTTTGACTAGTTCCACTGAC+AGG - chr1.1:71071597-71071616 None:intergenic 40.0%
!!! TTGTGGTTTTGGATGGTAAG+AGG - chr1.1:71072423-71072442 None:intergenic 40.0%
AACCGGCACAATATTCTCAC+CGG - chr1.1:71071196-71071215 None:intergenic 45.0%
AACGAATGTGGTAGGGTTTG+TGG - chr1.1:71072531-71072550 None:intergenic 45.0%
AATGTGGTAGGGTTTGTGGA+TGG - chr1.1:71072527-71072546 None:intergenic 45.0%
AGAACCAAAGAAGGTAGGAG+TGG + chr1.1:71072329-71072348 MS.gene76044:CDS 45.0%
AGAGAGCCAATGTTTCTTGG+CGG - chr1.1:71071326-71071345 None:intergenic 45.0%
AGCATGGAAGAAGATGAAGG+AGG + chr1.1:71073601-71073620 MS.gene76044:CDS 45.0%
CAAGTCTTAAGGGTCCACTT+TGG + chr1.1:71071385-71071404 MS.gene76044:CDS 45.0%
CCCTCTTCAAATGTAGTTGG+AGG - chr1.1:71073478-71073497 None:intergenic 45.0%
CTTGAAACCTCTTCCCAAAG+TGG - chr1.1:71071402-71071421 None:intergenic 45.0%
GAAGGAGAAACGAATGTGGT+AGG - chr1.1:71072539-71072558 None:intergenic 45.0%
GAGAAAGGAAGAGAGATTGG+TGG + chr1.1:71071243-71071262 MS.gene76044:CDS 45.0%
GAGCTGCTGATGATCTTTCT+TGG - chr1.1:71072868-71072887 None:intergenic 45.0%
GCACCCTCTTCAAATGTAGT+TGG - chr1.1:71073481-71073500 None:intergenic 45.0%
GGTAGGAGTGGAAAATCAGA+AGG + chr1.1:71072341-71072360 MS.gene76044:CDS 45.0%
TAGAGGGATTAAGGAAGTGG+AGG - chr1.1:71072215-71072234 None:intergenic 45.0%
TCAGCTGCAATGAAGAGACA+AGG + chr1.1:71073205-71073224 MS.gene76044:CDS 45.0%
TGGAACCTATTTCCTGTCAG+TGG + chr1.1:71071582-71071601 MS.gene76044:intron 45.0%
TGGTGAAGGAGAAACGAATG+TGG - chr1.1:71072543-71072562 None:intergenic 45.0%
! CACTTTGGGAAGAGGTTTCA+AGG + chr1.1:71071400-71071419 MS.gene76044:CDS 45.0%
! GTAATGATGGAGACTTGCTC+CGG + chr1.1:71071174-71071193 MS.gene76044:CDS 45.0%
! GTGGCTGTCCAGACAATTTT+TGG - chr1.1:71072919-71072938 None:intergenic 45.0%
!! TACATTTGAAGAGGGTGCTG+TGG + chr1.1:71073483-71073502 MS.gene76044:CDS 45.0%
!! TTAACGGTGGTGGAGGAAAT+AGG + chr1.1:71071295-71071314 MS.gene76044:CDS 45.0%
!!! TCACTTTTTGGCCACCTTGA+TGG - chr1.1:71073100-71073119 None:intergenic 45.0%
AAGTGGAGGAGACAGAAAGG+AGG - chr1.1:71072201-71072220 None:intergenic 50.0%
AGAGCCAATGTTTCTTGGCG+GGG - chr1.1:71071324-71071343 None:intergenic 50.0%
AGGAAGTGGAGGAGACAGAA+AGG - chr1.1:71072204-71072223 None:intergenic 50.0%
AGGGTTTGTGGATGGAACAG+TGG - chr1.1:71072519-71072538 None:intergenic 50.0%
CTCCGGTGAGAATATTGTGC+CGG + chr1.1:71071191-71071210 MS.gene76044:CDS 50.0%
CTTCTCCTTCTCCATCAAGG+TGG + chr1.1:71073086-71073105 MS.gene76044:CDS 50.0%
GAGAGCCAATGTTTCTTGGC+GGG - chr1.1:71071325-71071344 None:intergenic 50.0%
GTGCTTCTCCTTCTCCATCA+AGG + chr1.1:71073083-71073102 MS.gene76044:CDS 50.0%
TGAGACGATGGAGCCATTGA+TGG + chr1.1:71073429-71073448 MS.gene76044:CDS 50.0%
TGGTGCAGCCAGAACAACAA+TGG + chr1.1:71073449-71073468 MS.gene76044:CDS 50.0%
TTTGGCCACCTTGATGGAGA+AGG - chr1.1:71073094-71073113 None:intergenic 50.0%
! GGAGGTCTCCATTGTTGTTC+TGG - chr1.1:71073460-71073479 None:intergenic 50.0%
! TAAGGGTCCACTTTGGGAAG+AGG + chr1.1:71071392-71071411 MS.gene76044:CDS 50.0%
! TCTTCCCACAATGGCGCTTT+TGG - chr1.1:71073181-71073200 None:intergenic 50.0%
! TGTTCTGGCTGCACCATCAA+TGG - chr1.1:71073445-71073464 None:intergenic 50.0%
!! AGGAAGAGAGATTGGTGGTG+AGG + chr1.1:71071248-71071267 MS.gene76044:CDS 50.0%
CAAGTCTTCGTCCGAAGCTG+TGG - chr1.1:71072648-71072667 None:intergenic 55.0%
CACAGCTTCGGACGAAGACT+TGG + chr1.1:71072646-71072665 MS.gene76044:CDS 55.0%
GATGCCAGAGCTGTCTGACA+CGG - chr1.1:71072974-71072993 None:intergenic 55.0%
!! ACGGTGGTGGAGGAAATAGG+TGG + chr1.1:71071298-71071317 MS.gene76044:CDS 55.0%
!! ATGGGCCAAAAGCGCCATTG+TGG + chr1.1:71073173-71073192 MS.gene76044:CDS 55.0%
!! TGGGCCAAAAGCGCCATTGT+GGG + chr1.1:71073174-71073193 MS.gene76044:CDS 55.0%
GTCTCCGTGTCAGACAGCTC+TGG + chr1.1:71072967-71072986 MS.gene76044:CDS 60.0%
GTCTGACACGGAGACAGCTC+TGG - chr1.1:71072962-71072981 None:intergenic 60.0%
GTGGCCCCGCCAAGAAACAT+TGG + chr1.1:71071317-71071336 MS.gene76044:CDS 60.0%
GTTACGGCGCAAAACGGAGG+CGG + chr1.1:71071213-71071232 MS.gene76044:CDS 60.0%
! CCTCCGTTTTGCGCCGTAAC+CGG - chr1.1:71071213-71071232 None:intergenic 60.0%
!! GTGCCGGTTACGGCGCAAAA+CGG + chr1.1:71071207-71071226 MS.gene76044:CDS 60.0%
CCGGTTACGGCGCAAAACGG+AGG + chr1.1:71071210-71071229 MS.gene76044:CDS 65.0%
GGAGGCGGCGGTGAAGAGAA+AGG + chr1.1:71071228-71071247 MS.gene76044:CDS 65.0%
! CACGGAGACAGCTCTGGTGC+TGG - chr1.1:71072956-71072975 None:intergenic 65.0%
! GGAGACAGCTCTGGTGCTGG+CGG - chr1.1:71072953-71072972 None:intergenic 65.0%
!! TCTGGTGCTGGCGGTGGTTG+TGG - chr1.1:71072944-71072963 None:intergenic 65.0%
ACGGCGCAAAACGGAGGCGG+CGG + chr1.1:71071216-71071235 MS.gene76044:CDS 70.0%
GCTGGCGGTGGTTGTGGTGG+TGG - chr1.1:71072938-71072957 None:intergenic 70.0%
!! GACAGCTCTGGTGCTGGCGG+TGG - chr1.1:71072950-71072969 None:intergenic 70.0%
!! GGTGCTGGCGGTGGTTGTGG+TGG - chr1.1:71072941-71072960 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 71071153 71073687 71071153 ID=MS.gene76044
chr1.1 mRNA 71071153 71073687 71071153 ID=MS.gene76044.t1;Parent=MS.gene76044
chr1.1 exon 71071153 71071421 71071153 ID=MS.gene76044.t1.exon1;Parent=MS.gene76044.t1
chr1.1 CDS 71071153 71071421 71071153 ID=cds.MS.gene76044.t1;Parent=MS.gene76044.t1
chr1.1 exon 71072250 71073687 71072250 ID=MS.gene76044.t1.exon2;Parent=MS.gene76044.t1
chr1.1 CDS 71072250 71073687 71072250 ID=cds.MS.gene76044.t1;Parent=MS.gene76044.t1
Gene Sequence

>MS.gene76044

ATGCAGCTTGGTGATGCAACAGTAATGATGGAGACTTGCTCCGGTGAGAATATTGTGCCGGTTACGGCGCAAAACGGAGGCGGCGGTGAAGAGAAAGGAAGAGAGATTGGTGGTGAGGAAGATGATAAGATGAATATCAATATTAACGGTGGTGGAGGAAATAGGTGGCCCCGCCAAGAAACATTGGCTCTCTTGAAGATAAGATCAGATATGGATGGTGTTTTTCGTGATTCAAGTCTTAAGGGTCCACTTTGGGAAGAGGTTTCAAGGTATCCTCAATTCAATATTTATATATCTATGTCTTTTCTTTTTGTTTTCTTCTTCTTCTTCATCTTTTTCTATATACCTTTCATTTTCTTGTTTATAAGCTATATCATGCATGAAGAAGATCTGAATTTAGATATGTATCCAAATTAACCAATCAGTTTTTGGAACCTATTTCCTGTCAGTGGAACTAGTCAAAAAAAGCTCTTTGATTGGAATCATTTTTTTTTTCCTTCTGAAAAGGCATATATCTTATTCTCAAGTGTATGAGGCAATAATTCAGTTATCTTAGATTTTGAGGCATAAGATAATTTATGTCATTTGAATCATCTAAGTGAAATTCTTCTATCTGAATCTTGCTGGATGTTGAACTCAATCATAGTTTATAATTTACTTCTCCATATATAATTTTTTTTTGGTACATACTTCTTCATATATAATTTTATTAACCAATTAAATTACTTAAGTTTAATGTTGAGTTCATCTTCTTCAACTTTTTTTAGGTTAATTTCATCCTTCCATCTAATTTTTATTACTTTGTGTGTTTCTTATTTATTGGTCACTCTTTTAGGTATGAACTACCTAGCTTCATGTGTTTTTTTTTTTTTTTTTGGTGTAACATGTGTTTTAATTTAGTACTATATACTTTGTATTTTTCCTCATGACAAATTTTTACTTTTTTTACGTGAAAGGAGTCTAGTTTTTTCTTTTCCTTTTTCAATGGATATACTAACTATATATGATTACAAAATTCAATCATCATTATCAATATTAATTATCACCTCCTTTCTGTCTCCTCCACTTCCTTAATCCCTCTATATATATACATGTAGGAAACTAGCTGAACTTGGCTATCATAGAAGCTCAAAGAAGTGCAAGGAGAAATTTGAAAATGTATACAAGTATCACAAAAGAACCAAAGAAGGTAGGAGTGGAAAATCAGAAGGAAAAACCTATAGATTTTTTGATCAATTACAAGCACTTGAAAAACAACTCACATTCTCCTCTTACCATCCAAAACCACAACCAAATAATAACACACTCTCATCACCAACAACAAATCCCAATGAAACCACACAAACAATATCATATGTCACAACCACTGTTCCATCCACAAACCCTACCACATTCGTTTCTCCTTCACCACAACCTAACAACAACAATGTTCCAAATTCTTTGCCAAACATGAACAACCTATTCTCAACCACAACAACTTCAACTTCTTCTTCCACAGCTTCGGACGAAGACTTGGAAGAGAAGTATAGAAAGAAGAGAAAATGGAAGGACTATTTCAGGAGACTCACAAGACAAGTGCTTGCAAAACAAGAGGAAATGCAAAAGAAGTTTCTTGAGGCTATAGACAAACGTGAAAAAGAACACATTGCTCAACAAGATGCTTTGAGAATTCAAGAAATGGATAGAATCAATAAAGAACACGAACTTCTTATCCAAGAAAGATCATCAGCAGCTCAAAAAAATGCAGCTGTTATTGCATTTCTCCAAAAATTGTCTGGACAGCCACCACCACAACCACCGCCAGCACCAGAGCTGTCTCCGTGTCAGACAGCTCTGGCATCACAAGTTCAAACACAGCAACTAGTGATACCAAACAACAACATTGTTGAATTTCAAAATTTGAATAATGGTTACAAAAGTGGTAATGGTGGTGCTTCTCCTTCTCCATCAAGGTGGCCAAAAAGTGAAGTTCATGCTCTAATAAGGATAAGAACAAGTCTTGAACCAAAGTATCAAGAAAATGGGCCAAAAGCGCCATTGTGGGAAGATATTTCAGCTGCAATGAAGAGACAAGGATACAATAGGAATGCGAAAAGGTGCAAAGAAAAATGGGAGAATATCAACAAATACTACAAGAAAATGAAGGAAAGTAACAAGCAAAGGCGTGATGATAGTAAGACATGTCCTTATTTTAATGAACTTGAAGCTATTTACAAAGAAAAGAACAAAACACAGAACCTTTTTGGTTCTAATTCGTTTCATAGCATGAAGTCAAATGAGACGATGGAGCCATTGATGGTGCAGCCAGAACAACAATGGAGACCTCCAACTACATTTGAAGAGGGTGCTGTGGTGAAGAAGAATGTTGATGAAGCAAAAGAAGAAGATGACAATGGTGATGTTGATGATGAAGAAGATGAAAACATGGATGAATATGAAGATGGAGATAGCATGGAAGAAGATGAAGGAGGTGTTAGTAGCCGTTATGAAGTTGTGACAACAAATAAACTTTCATCAATGGACACAGTTGTATGA
Protein sequence

>MS.gene76044.t1

MQLGDATVMMETCSGENIVPVTAQNGGGGEEKGREIGGEEDDKMNININGGGGNRWPRQETLALLKIRSDMDGVFRDSSLKGPLWEEVSRKLAELGYHRSSKKCKEKFENVYKYHKRTKEGRSGKSEGKTYRFFDQLQALEKQLTFSSYHPKPQPNNNTLSSPTTNPNETTQTISYVTTTVPSTNPTTFVSPSPQPNNNNVPNSLPNMNNLFSTTTTSTSSSTASDEDLEEKYRKKRKWKDYFRRLTRQVLAKQEEMQKKFLEAIDKREKEHIAQQDALRIQEMDRINKEHELLIQERSSAAQKNAAVIAFLQKLSGQPPPQPPPAPELSPCQTALASQVQTQQLVIPNNNIVEFQNLNNGYKSGNGGASPSPSRWPKSEVHALIRIRTSLEPKYQENGPKAPLWEDISAAMKRQGYNRNAKRCKEKWENINKYYKKMKESNKQRRDDSKTCPYFNELEAIYKEKNKTQNLFGSNSFHSMKSNETMEPLMVQPEQQWRPPTTFEEGAVVKKNVDEAKEEDDNGDVDDEEDENMDEYEDGDSMEEDEGGVSSRYEVVTTNKLSSMDTVV